1,693 results match your criteria Venezuelan Encephalitis


Development of a Bead-based Immunoassay Using Virus-like Particles for Detection of Alphaviral Humoral Response.

J Virol Methods 2019 Apr 15. Epub 2019 Apr 15.

Diagnostics Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA. Electronic address:

There is a pressing need for sustainable and sensitive immunodiagnostics for use in public health efforts to understand and combat the threat of endemic and emerging infectious diseases. In this proof-of-concept work, we describe an immunodiagnostic approach based on the utilization of virus-like particles (VLPs) in a magnetic bead-based platform for multiplexed detection of antiviral humoral response. A retroviral-based VLP, that presents Venezuelan equine encephalitis virus E1/E2 glycoprotein antigen on its surface, was synthesized and coupled to magnetic beads to create VLP-conjugated microspheres (VCMs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jviromet.2019.04.013DOI Listing

Efficacy of a ML336 derivative against Venezuelan and eastern equine encephalitis viruses.

Antiviral Res 2019 Apr 7;167:25-34. Epub 2019 Apr 7.

Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin, Madison, WI, 53705-2222, USA. Electronic address:

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01663542193001
Publisher Site
http://dx.doi.org/10.1016/j.antiviral.2019.04.004DOI Listing
April 2019
4 Reads

Lack of Evidence of Sylvatic Transmission of Dengue Viruses in the Amazon Rainforest Near Iquitos, Peru.

Vector Borne Zoonotic Dis 2019 Apr 9. Epub 2019 Apr 9.

3 Department of Viral and Rickettsial Diseases, Naval Medical Research Center, Silver Spring, Maryland.

Dengue viruses (DENV) are currently responsible for more human morbidity and mortality than any other known arbovirus, and all four DENV are known to exist in sylvatic cycles that might allow these viruses to persist if the urban (Aedes aegypti) cycle could be controlled. To determine whether DENV were being maintained in a sylvatic cycle in a forested area about 14 km southwest of Iquitos, Peru, a city in which all 4 serotypes of DENV circulate, we placed 20 DENV seronegative Aotus monkeys in cages either in the canopy or near ground level for a total of 125.6 months. Read More

View Article

Download full-text PDF

Source
https://www.liebertpub.com/doi/10.1089/vbz.2018.2408
Publisher Site
http://dx.doi.org/10.1089/vbz.2018.2408DOI Listing
April 2019
7 Reads

Viral RNA-dependent RNA polymerase mutants display an altered mutation spectrum resulting in attenuation in both mosquito and vertebrate hosts.

PLoS Pathog 2019 04 4;15(4):e1007610. Epub 2019 Apr 4.

Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America.

The presence of bottlenecks in the transmission cycle of many RNA viruses leads to a severe reduction of number of virus particles and this occurs multiple times throughout the viral transmission cycle. Viral replication is then necessary for regeneration of a diverse mutant swarm. It is now understood that any perturbation of the mutation frequency either by increasing or decreasing the accumulation of mutations in an RNA virus results in attenuation of the virus. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.ppat.1007610DOI Listing
April 2019
2 Reads

Src Family Kinase Inhibitors Block Translation of Alphavirus Subgenomic mRNAs.

Antimicrob Agents Chemother 2019 Apr 27;63(4). Epub 2019 Mar 27.

Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA

Alphaviruses are arthropod-transmitted RNA viruses that can cause arthralgia, myalgia, and encephalitis in humans. Since the role of cellular kinases in alphavirus replication is unknown, we profiled kinetic changes in host kinase abundance and phosphorylation following chikungunya virus (CHIKV) infection of fibroblasts. Based upon the results of this study, we treated CHIKV-infected cells with kinase inhibitors targeting the Src family kinase (SFK)-phosphatidylinositol 3-kinase (PI3K)-AKT-mTORC signaling pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/AAC.02325-18DOI Listing
April 2019
4.476 Impact Factor

Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose.

J Struct Biol 2019 Apr 27;206(1):119-127. Epub 2019 Feb 27.

Department of Pharmacy, University of Patras, GR-26504, Greece. Electronic address:

Venezuelan equine encephalitis virus (VEEV) is a new world alphavirus which can be involved in several central nervous system disorders such as encephalitis and meningitis. The VEEV genome codes for 4 non-structural proteins (nsP), of which nsP3 contains a Macro domain. Macro domains (MD) can be found as stand-alone proteins or embedded within larger proteins in viruses, bacteria and eukaryotes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jsb.2019.02.008DOI Listing
April 2019
5 Reads

Novel RU486 (mifepristone) analogues with increased activity against Venezuelan Equine Encephalitis Virus but reduced progesterone receptor antagonistic activity.

Sci Rep 2019 Feb 22;9(1):2634. Epub 2019 Feb 22.

Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology School of Biomedical Sciences, Monash University, Melbourne, Australia.

There are currently no therapeutics to treat infection with the alphavirus Venezuelan equine encephalitis virus (VEEV), which causes flu-like symptoms leading to neurological symptoms in up to 14% of cases. Large outbreaks of VEEV can result in 10,000 s of human cases and mass equine death. We previously showed that mifepristone (RU486) has anti-VEEV activity (EC = 20 μM) and only limited cytotoxicity (CC > 100 μM), but a limitation in its use is its abortifacient activity resulting from its ability to antagonize the progesterone receptor (PR). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-38671-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385310PMC
February 2019
2 Reads

Current Understanding of the Molecular Basis of Venezuelan Equine Encephalitis Virus Pathogenesis and Vaccine Development.

Viruses 2019 Feb 18;11(2). Epub 2019 Feb 18.

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Venezuelan equine encephalitis virus (VEEV) is an alphavirus in the family Togaviridae. VEEV is highly infectious in aerosol form and a known bio-warfare agent that can cause severe encephalitis in humans. Periodic outbreaks of VEEV occur predominantly in Central and South America. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/v11020164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410161PMC
February 2019
1 Read

Self-Amplifying RNA Vaccines for Venezuelan Equine Encephalitis Virus Induce Robust Protective Immunogenicity in Mice.

Mol Ther 2019 Apr 7;27(4):850-865. Epub 2019 Jan 7.

GSK, Rockville, MD 20850, USA. Electronic address:

Venezuelan equine encephalitis virus (VEEV) is a known biological defense threat. A live-attenuated investigational vaccine, TC-83, is available, but it has a high non-response rate and can also cause severe reactogenicity. We generated two novel VEE vaccine candidates using self-amplifying mRNA (SAM). Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S15250016193000
Publisher Site
http://dx.doi.org/10.1016/j.ymthe.2018.12.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453513PMC
April 2019
12 Reads

Proteolytic cleavage of host proteins by the Group IV viral proteases of Venezuelan equine encephalitis virus and Zika virus.

Antiviral Res 2019 Apr 10;164:106-122. Epub 2019 Feb 10.

Center for Bio/molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC 20375, USA. Electronic address:

The alphaviral nonstructural protein 2 (nsP2) cysteine proteases (EC 3.4.22. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.antiviral.2019.02.001DOI Listing
April 2019
4 Reads
3.938 Impact Factor

Human cathelicidin peptide LL-37 as a therapeutic antiviral targeting Venezuelan equine encephalitis virus infections.

Antiviral Res 2019 Apr 8;164:61-69. Epub 2019 Feb 8.

National Center for Biodefense and Infectious Disease, School of Systems Biology, George Mason University, Manassas, VA, USA.

Venezuelan equine encephalitis virus (VEEV), a new world alphavirus belonging to the Togaviridae family, causes periodic disease outbreaks in humans and equines with high associated mortality and morbidity. VEEV is highly infectious via the aerosol route and so has been developed as a biological weapon (Hawley and Eitzen, 2001). Despite its current classification as a category B select agent, there are no FDA approved vaccines or therapeutics to counter VEEV infections. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01663542183068
Publisher Site
http://dx.doi.org/10.1016/j.antiviral.2019.02.002DOI Listing
April 2019
18 Reads

[The use of genetic engineering constructions as control samples on evaluation of diagnostic kits for reveal of rna of hazard and extremely hazard agents of virus infections by reverse transcription - polymerase chain reaction.]

Klin Lab Diagn 2018;63(6):372-375

Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of the Defens of the Russian Federation, 141306, Sergiev Posad, Russian Federation.

The reverse transcription - polymerase chain reaction method (RT-PCR) has leading position on diagnostic infections, caused by RNA-containing viruses. This method presents severe requirements to carrying out of everybody stages of analysis (extraction of nucleic acid, carry out reverse transcription, amplification of DNA). It is necessary to account the possibility of false positive or false negative results appearance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.18821/0869-2084-2018-63-6-372-375DOI Listing
January 2018
3 Reads

New World alphavirus protein interactomes from a therapeutic perspective.

Antiviral Res 2019 Mar 26;163:125-139. Epub 2019 Jan 26.

National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, VA, USA. Electronic address:

The New World alphaviruses, Venezuelan, eastern and western equine encephalitis viruses (VEEV, EEEV, and WEEV), are important human pathogens due to their ability to cause varying levels of morbidity and mortality in humans. There is also concern about VEEV and EEEV being used as bioweapons. Currently, a FDA-approved antiviral is lacking for New World alphaviruses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.antiviral.2019.01.015DOI Listing
March 2019
1 Read

Antiviral Protection by IFITM3 In Vivo.

Curr Clin Microbiol Rep 2018 Dec 3;5(4):229-237. Epub 2018 Aug 3.

Department of Microbial Infection and Immunity, Infectious, Diseases Institute, The Ohio State University, 460 W 12th Ave, Biomedical Research Tower 790, Columbus, OH 43210, USA.

Purpose Of Review: Interferon-induced transmembrane protein 3 (IFITM3) is a cellular restriction factor that blocks fusion between virus and host membranes. Here, we provide an introduction to IFITM3 and the biochemical regulation underlying its antiviral activity. Further, we analyze and summarize the published literature examining phenotypes of IFITM3 knockout mice upon infections with viral pathogens and discuss the controversial association between single nucleotide polymorphisms (SNPs) in the human gene and severe virus infections. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s40588-018-0103-0
Publisher Site
http://dx.doi.org/10.1007/s40588-018-0103-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334760PMC
December 2018
13 Reads

Approved drugs screening against the nsP1 capping enzyme of Venezuelan equine encephalitis virus using an immuno-based assay.

Antiviral Res 2019 Mar 11;163:59-69. Epub 2019 Jan 11.

Aix Marseille Université, CNRS, AFMB UMR 7257, Marseille, France; Unité des Virus Emergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), Marseille, France. Electronic address:

Alphaviruses such as the Venezuelan equine encephalitis virus (VEEV) are important human emerging pathogens transmitted by mosquitoes. They possess a unique viral mRNA capping mechanism catalyzed by the viral non-structural protein nsP1, which is essential for virus replication. The alphaviruses capping starts by the methylation of a GTP molecule by the N7-guanine methyltransferase (MTase) activity; nsP1 then forms a covalent link with mGMP releasing pyrophosphate (GT reaction) and the mGMP is next transferred onto the 5'-diphosphate end of the viral mRNA to form a cap-0 structure. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01663542183064
Publisher Site
http://dx.doi.org/10.1016/j.antiviral.2019.01.003DOI Listing
March 2019
16 Reads

Venezuelan Equine Encephalitis Virus Capsid Implicated in Infection-Induced Cell Cycle Delay .

Front Microbiol 2018 18;9:3126. Epub 2018 Dec 18.

National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, VA, United States.

Venezuelan equine encephalitis virus (VEEV) is a positive sense, single-stranded RNA virus and member of the New World alphaviruses. It causes a biphasic febrile illness that can be accompanied by central nervous system involvement and moderate morbidity in humans and severe mortality in equines. The virus has a history of weaponization, lacks FDA-approved therapeutics and vaccines in humans, and is considered a select agent. Read More

View Article

Download full-text PDF

Source
https://www.frontiersin.org/article/10.3389/fmicb.2018.03126
Publisher Site
http://dx.doi.org/10.3389/fmicb.2018.03126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315117PMC
December 2018
9 Reads

Eco-epidemiology of the Venezuelan equine encephalitis virus in bats of Córdoba and Sucre, Colombia.

Acta Trop 2019 Mar 19;191:178-184. Epub 2018 Dec 19.

Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia. Electronic address:

Alphavirus infection associated encephalitis is an emerging infectious disease with a high impact on public health in Latin America.

Objective: To study the eco-epidemiology of alphaviruses in bats of departments of Córdoba and Sucre, Colombia.

Methodology: A prospective descriptive cross-sectional study with a non-probabilistic sampling, in 12 localities of Córdoba and Sucre was carried out. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actatropica.2018.12.016DOI Listing
March 2019
8 Reads

Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development.

PLoS One 2018 17;13(12):e0208851. Epub 2018 Dec 17.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

In response to the aggressive global spread of the mosquito-borne chikungunya virus (CHIKV), an accurate and accessible diagnostic tool is of high importance. CHIKV, an arthritogenic alphavirus, comprises three genotypes: East/Central/South African (ECSA), West African (WA), and Asian. A previous rapid immunochromatographic (IC) test detecting CHIKV E1 protein showed promising performance for detection of the ECSA genotype. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208851PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296674PMC
December 2018
5 Reads

Induction of Expandable Adipose-Derived Mesenchymal Stem Cells from Aged Mesenchymal Stem Cells by a Synthetic Self-Replicating RNA.

Int J Mol Sci 2018 Nov 6;19(11). Epub 2018 Nov 6.

Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.

Adipose-derived mesenchymal stem cells (ADSCs) have attracted attention due to their potential for use in the treatment of various diseases. However, the self-renewal capacity of ADSCs is restricted and their function diminishes during passage. We previously generated induced tissue-specific stem cells from mouse pancreatic cells using a single synthetic self-replicating Venezuelan Equine Encephalitis (VEE)-reprogramming factor (RF) RNA replicon (SR-RNA) expressing the reprogramming factors POU class 5 homeobox 1 (OCT4), Krueppel-like factor 4 (KLF4), Sex determining region Y-box 2 (SOX2), and Glis Family Zinc Finger 1 (GLIS1). Read More

View Article

Download full-text PDF

Source
http://www.mdpi.com/1422-0067/19/11/3489
Publisher Site
http://dx.doi.org/10.3390/ijms19113489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274871PMC
November 2018
2 Reads

Mitochondrial-Directed Antioxidant Reduces Microglial-Induced Inflammation in Murine In Vitro Model of TC-83 Infection.

Viruses 2018 11 2;10(11). Epub 2018 Nov 2.

National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, USA.

Venezuelan equine encephalitis virus (VEEV) is an arbovirus that is associated with robust inflammation that contributes to neurodegenerative phenotypes. In addition to triggering central nervous system (CNS) inflammation, VEEV will also induce mitochondrial dysfunction, resulting in increased cellular apoptosis. In this study, we utilize the TC-83 strain of VEEV to determine the role of mitochondrial oxidative stress in mediating inflammation elicited by murine brain microglial cells. Read More

View Article

Download full-text PDF

Source
http://www.mdpi.com/1999-4915/10/11/606
Publisher Site
http://dx.doi.org/10.3390/v10110606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266753PMC
November 2018
4 Reads

Zoonotic Viral Diseases of Equines and Their Impact on Human and Animal Health.

Open Virol J 2018 31;12:80-98. Epub 2018 Aug 31.

ICAR-National Research Centre on Equines, Hisar-125001, India.

Introduction: Zoonotic diseases are the infectious diseases that can be transmitted to human beings and vice versa from animals either directly or indirectly. These diseases can be caused by a range of organisms including bacteria, parasites, viruses and fungi. Viral diseases are highly infectious and capable of causing pandemics as evidenced by outbreaks of diseases like Ebola, Middle East Respiratory Syndrome, West Nile, SARS-Corona, Nipah, Hendra, Avian influenza and Swine influenza. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1874357901812010080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142672PMC
August 2018
12 Reads

Comparison of Aerosol- and Percutaneous-acquired Venezuelan Equine Encephalitis in Humans and Nonhuman Primates for Suitability in Predicting Clinical Efficacy under the Animal Rule.

Comp Med 2018 Oct 3;68(5):380-395. Epub 2018 Oct 3.

Joint Vaccine Acquisition Program, Medical Countermeasure Systems, Fort Detrick, Maryland.

Licensure of medical countermeasure vaccines to protect against aerosolized Venezuelan equine encephalitis virus (VEEV) requires the use of the Animal Rule to assess vaccine efficacy, because human studies are not feasible or ethical. We therefore performed a retrospective study of VEE cases that occurred in at-risk laboratory workers and support personnel during the United States Biowarfare Program (1943-1969) to better define percutaneous- and aerosol-acquired VEE in humans and to compare these results with those described for the NHP model (in which high-dose aerosol VEEV challenge led to more severe encephalitis than parenteral challenge). Record review and analysis of 17 aerosol- and 23 percutaneous-acquired human cases of VEE included incubation period, symptoms, physical examination findings, and markers of infection. Read More

View Article

Download full-text PDF

Source
http://www.ingentaconnect.com/content/10.30802/AALAS-CM-18-0
Publisher Site
http://dx.doi.org/10.30802/AALAS-CM-18-000027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200028PMC
October 2018
12 Reads

The genetic adjuvant IL-12 enhances the protective efficacy of a DNA vaccine for Venezuelan equine encephalitis virus delivered by intramuscular injection in mice.

Antiviral Res 2018 Nov 27;159:113-121. Epub 2018 Sep 27.

U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. Electronic address:

We have previously shown that DNA vaccines expressing codon-optimized alphavirus envelope glycoprotein genes protect both mice and non-human primates from viral challenge when delivered by intramuscular electroporation (IM-EP). To determine if we could achieve equivalent immunogenicity and protective efficacy in the absence of electroporation, we co-delivered our Venezuelan equine encephalitis virus (VEEV) DNA vaccine with DNA plasmids expressing genetic adjuvants designed to augment immune responses. We tested the T1-inducing cytokine IL-12 as well as the granulocyte growth factor GM-CSF, both of which have demonstrated significant adjuvant effect when included in clinical DNA vaccine formulations. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01663542183037
Publisher Site
http://dx.doi.org/10.1016/j.antiviral.2018.09.014DOI Listing
November 2018
8 Reads

The Interferon-Induced Exonuclease ISG20 Exerts Antiviral Activity through Upregulation of Type I Interferon Response Proteins.

mSphere 2018 09 19;3(5). Epub 2018 Sep 19.

Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Type I interferon (IFN)-stimulated genes (ISGs) have critical roles in inhibiting virus replication and dissemination. Despite advances in understanding the molecular basis of ISG restriction, the antiviral mechanisms of many remain unclear. The 20-kDa ISG ISG20 is a nuclear 3'-5' exonuclease with preference for single-stranded RNA (ssRNA) and has been implicated in the IFN-mediated restriction of several RNA viruses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/mSphere.00209-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147134PMC
September 2018
5 Reads

Lipid-Coated Mesoporous Silica Nanoparticles for the Delivery of the ML336 Antiviral to Inhibit Encephalitic Alphavirus Infection.

Sci Rep 2018 Sep 18;8(1):13990. Epub 2018 Sep 18.

Department of Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA, USA.

Venezuelan equine encephalitis virus (VEEV) poses a major public health risk due to its amenability for use as a bioterrorism agent and its severe health consequences in humans. ML336 is a recently developed chemical inhibitor of VEEV, shown to effectively reduce VEEV infection in vitro and in vivo. However, its limited solubility and stability could hinder its clinical translation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-32033-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143628PMC
September 2018
7 Reads

Author Correction: Novel inhibitors targeting Venezuelan equine encephalitis virus capsid protein identified using In Silico Structure-Based-Drug-Design.

Sci Rep 2018 Sep 4;8(1):13487. Epub 2018 Sep 4.

Nuclear Signaling Laboratory, Department of Biochemistry and Molecular Biology School of Biomedical Sciences, Monash University, Melbourne, Australia.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-31644-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120891PMC
September 2018
5 Reads

Arboviruses and pregnancy: maternal, fetal, and neonatal effects.

Lancet Child Adolesc Health 2017 Oct 10;1(2):134-146. Epub 2017 Aug 10.

Institut Pasteur, Biology of Infection Unit, Paris, France; Inserm U1117, Paris, France; Paris-Descartes University, Sorbonne Paris Cité, Centre d'Infectiologie Necker-Pasteur, Necker-Enfants Malades University Hospital, Institut Imagine, Assistance Publique-Hôpitaux de Paris, Paris, France. Electronic address:

Arboviruses are an expanding public health threat, with pregnant women facing unique complications from arbovirus infections. These infections, such as dengue and Crimean-Congo haemorrhagic fever, can be more severe in pregnant women than in the general population. Vertical transmission is reported for many arboviruses and can severely affect pregnancy outcome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/S2352-4642(17)30021-4DOI Listing
October 2017
56 Reads

Genetic control of alphavirus pathogenesis.

Mamm Genome 2018 08 27;29(7-8):408-424. Epub 2018 Aug 27.

Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Alphaviruses, members of the positive-sense, single-stranded RNA virus family Togaviridae, represent a re-emerging public health concern worldwide as mosquito vectors expand into new geographic ranges. Members of the alphavirus genus tend to induce clinical disease characterized by rash, arthralgia, and arthritis (chikungunya virus, Ross River virus, and Semliki Forest virus) or encephalomyelitis (eastern equine encephalitis virus, western equine encephalitis virus, and Venezuelan equine encephalitis virus), though some patients who recover from the initial acute illness may develop long-term sequelae, regardless of the specific infecting virus. Studies examining the natural disease course in humans and experimental infection in cell culture and animal models reveal that host genetics play a major role in influencing susceptibility to infection and severity of clinical disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00335-018-9776-1DOI Listing
August 2018
30 Reads

Direct and indirect pro-inflammatory cytokine response resulting from TC-83 infection of glial cells.

Virulence 2018 ;9(1):1403-1421

a National Center for Biodefense and Infectious Diseases , George Mason University , Manassas , VA , USA.

Venezuelan equine encephalitis virus (VEEV) is a neurotropic arbovirus that is highly infectious as an aerosol and can result in an encephalitic phenotype in infected individuals. VEEV infections are known to be associated with robust inflammation that eventually contributes to neurodegenerative phenotypes. In this study, we utilize the TC-83 strain of VEEV, which is known to induce the expression of IL-6, IL-8, and other pro-inflammatory cytokines. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21505594.2018.1509668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141141PMC
April 2019
3 Reads

Protective efficacy of monovalent and trivalent recombinant MVA-based vaccines against three encephalitic alphaviruses.

Vaccine 2018 08 20;36(34):5194-5203. Epub 2018 Jul 20.

Defence Research and Development Canada, Suffield Research Centre, Box 4000, Station Main, Medicine Hat, AB T1A 8K6, Canada.

The three encephalitic alphaviruses, western, eastern, and Venezuelan equine encephalitis viruses (WEEV, EEEV, and VEEV) are potential biothreat agents due to high infectivity through aerosol exposure, ease of production in large amounts, and relative stability in the environment. Currently, there is no licensed vaccine for human use to these three encephalitic alphaviruses, and efforts to move vaccine candidates forward into clinical trials have not been successful. In this study, the modified vaccinia Ankara-Bavarian Nordic (MVA-BN®) vaccine platform was used to construct and produce three monovalent recombinant MVA-BN-based encephalitic alphavirus vaccines, MVA-BN-W, MVA-BN-E, and MVA-BN-V. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vaccine.2018.06.064DOI Listing
August 2018
3 Reads

Isolation of Complete Equine Encephalitis Virus Genome from Human Swab Specimen, Peru.

Emerg Infect Dis 2018 08;24(8):1578-1580

While studying respiratory infections in Peru, we identified Venezuelan equine encephalitis virus (VEEV) in a nasopharyngeal swab, indicating that this alphavirus can be present in human respiratory secretions. Because VEEV may be infectious when aerosolized, our finding is relevant for the management of VEEV-infected patients and for VEEV transmission studies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3201/eid2408.171274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056129PMC
August 2018
13 Reads

Repurposed FDA-Approved drug sorafenib reduces replication of Venezuelan equine encephalitis virus and other alphaviruses.

Antiviral Res 2018 Sep 5;157:57-67. Epub 2018 Jul 5.

National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, VA, USA. Electronic address:

The New World alphaviruses -Venezuelan, eastern, and western equine encephalitis viruses (VEEV, EEEV, and WEEV respectively) - cause a febrile disease that is often lethal in equines and children and leads to long-term neurological sequelae in survivors. Endemic to the Americas, epizootic outbreaks of the three viruses occur sporadically in the continental United States. All three viruses aerosolize readily, replicate to high titers in cell culture, and have low infectious doses. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01663542183006
Publisher Site
http://dx.doi.org/10.1016/j.antiviral.2018.07.005DOI Listing
September 2018
7 Reads

A Multiagent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus-Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges.

J Immunol Res 2018 3;2018:8521060. Epub 2018 Jun 3.

United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.

There remains a need for vaccines that can safely and effectively protect against the biological threat agents Venezuelan (VEEV), western (WEEV), and eastern (EEEV) equine encephalitis virus. Previously, we demonstrated that a VEEV DNA vaccine that was optimized for increased antigen expression and delivered by intramuscular (IM) electroporation (EP) elicited robust and durable virus-specific antibody responses in multiple animal species and provided complete protection against VEEV aerosol challenge in mice and nonhuman primates. Here, we performed a comparative evaluation of the immunogenicity and protective efficacy of individual optimized VEEV, WEEV, and EEEV DNA vaccines with that of a 1 : 1 : 1 mixture of these vaccines, which we have termed the 3-EEV DNA vaccine, when delivered by IM EP. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2018/8521060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008678PMC
October 2018
25 Reads

Did Zika Virus Mutate to Cause Severe Outbreaks?

Trends Microbiol 2018 10 11;26(10):877-885. Epub 2018 Jun 11.

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.

Zika virus (ZIKV) has challenged the assumed knowledge regarding the pathobiology of flaviviruses. Despite causing sporadic and mild disease in the 50 years since its discovery, Zika virus has now caused multiple outbreaks in dozens of countries worldwide. Moreover, the disease severity in recent outbreaks, with neurological disease in adult and devastating congenital malformations in fetuses, was not previously seen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tim.2018.05.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160291PMC
October 2018
13 Reads

Structural Characterization and Formulation Development of a Trivalent Equine Encephalitis Virus-Like Particle Vaccine Candidate.

J Pharm Sci 2018 10 5;107(10):2544-2558. Epub 2018 Jun 5.

Macromolecule and Vaccine Stabilization Center, Department of Pharmaceutical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, Kansas 66047. Electronic address:

The zoonotic equine encephalitis viruses (EEVs) can cause debilitating and life-threatening disease, leading to ongoing vaccine development efforts for an effective virus-like particle (VLP) vaccine based on 3 strains of EEV (Eastern, Western, and Venezuelan or EEE, WEE and VEE VLPs, respectively). In this work, transmission electron microscopy and light scattering studies showed enveloped, spherical, and ∼70 nm sized VLPs. Biophysical studies demonstrated optimal VLP physical stability in the pH range of 7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.xphs.2018.05.022DOI Listing
October 2018
13 Reads

Exploration of (hetero)aryl Derived Thienylchalcones for Antiviral and Anticancer Activities.

Med Chem 2019 ;15(2):150-161

Pharmaceutical Sciences Department, College of Pharmacy, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064, United States.

Background: Search for new antiviral and anticancer agents are essential because of the emergence of drug resistance in recent years. In continuation of our efforts in identifying the new small molecule antiviral and anticancer agents, we identified chalcones as potent antiviral and anticancer agents.

Objective: With the aim of identifying the broad acting antiviral and anticancer agents, we discovered substituted aryl/heteroaryl derived thienyl chalcones as antiviral and anticancer agents. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1573406414666180524074648DOI Listing
March 2019
8 Reads
1.390 Impact Factor

Protein Phosphatase 1α Interacts with Venezuelan Equine Encephalitis Virus Capsid Protein and Regulates Viral Replication through Modulation of Capsid Phosphorylation.

J Virol 2018 08 17;92(15). Epub 2018 Jul 17.

National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, Virginia, USA

Protein phosphatase 1 (PP1) is a serine/threonine phosphatase which has been implicated in the regulation of a number of viruses, including HIV-1, Ebolavirus, and Rift Valley fever virus. Catalytic subunits of PP1 (PP1α, PP1β, and PP1γ) interact with a host of regulatory subunits and target a wide variety of cellular substrates through a combination of short binding motifs, including an RVxF motif present in the majority of PP1 regulatory subunits. Targeting the RVxF-interacting site on PP1 with the small molecule 1E7-03 inhibits HIV-1, Ebolavirus, and Rift Valley fever virus replication. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JVI.02068-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052284PMC
August 2018
10 Reads

Human and Equine Infection with Alphaviruses and Flaviviruses in Panamá during 2010: A Cross-Sectional Study of Household Contacts during an Encephalitis Outbreak.

Am J Trop Med Hyg 2018 Jun 3;98(6):1798-1804. Epub 2018 May 3.

Departments of Microbiology & Immunology and Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.

Members of the genera (family ) and (family ) are important zoonotic human and equine etiologic agents of neurologic diseases in the New World. In 2010, an outbreak of Madariaga virus (MADV; formerly eastern equine encephalitis virus) and Venezuelan equine encephalitis virus (VEEV) infections was reported in eastern Panamá. We further characterized the epidemiology of the outbreak by studying household contacts of confirmed human cases and of equine cases with neurological disease signs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4269/ajtmh.17-0679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086197PMC
June 2018
9 Reads
2.700 Impact Factor

Madariaga Virus: Identification of a Lineage III Strain in a Venezuelan Child With Acute Undifferentiated Febrile Illness, in the Setting of a Possible Equine Epizootic.

Clin Infect Dis 2018 Aug;67(4):619-621

Department of Infectious Diseases and Tropical Medicine, Instituto Diagnóstico Barquisimeto (IDB)/ Biomedical Research Institute/IDB Hospital, Barquisimeto, Lara.

We report identification of Madariaga virus (MADV) in plasma and urine samples from a child with acute undifferentiated febrile illness in Venezuela. Our data document the occurrence of milder MADV infections (ie, without encephalitis), with a symptom complex that resembles that seen with other arboviral infections, including dengue and zika. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/cid/ciy224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070041PMC
August 2018
30 Reads

History of Mosquitoborne Diseases in the United States and Implications for New Pathogens.

Emerg Infect Dis 2018 05;24(5):821-826

The introduction and spread of West Nile virus and the recent introduction of chikungunya and Zika viruses into the Americas have raised concern about the potential for various tropical pathogens to become established in North America. A historical analysis of yellow fever and malaria incidences in the United States suggests that it is not merely a temperate climate that keeps these pathogens from becoming established. Instead, socioeconomic changes are the most likely explanation for why these pathogens essentially disappeared from the United States yet remain a problem in tropical areas. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3201/eid2405.171609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938790PMC
May 2018
13 Reads

Alphaviruses: Serological Evidence of Human Infection in Paraguay (2012-2013).

Vector Borne Zoonotic Dis 2018 05 13;18(5):266-272. Epub 2018 Mar 13.

1 Research Institute in Health Sciences, National University of Asunción , San Lorenzo, Paraguay .

Introduction: Alphaviruses can produce febrile illness and encephalitis in dead-end hosts such as horses and humans. Within this genus, the Venezuelan Equine Encephalitis virus (VEEV) complex includes pathogenic epizootic subtypes and enzootic subtypes that are not pathogenic in horses (except subtype IE, Mexican strains), although they can cause febrile symptoms in humans. The Rio Negro virus (RNV-VEEV subtype VI) circulates in Argentina, where it was associated with undifferentiated febrile illness. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/vbz.2017.2178DOI Listing
May 2018
9 Reads

Genomic Characterization and Seroprevalence Studies on Alphaviruses in Uruguay.

Am J Trop Med Hyg 2018 Jun 5;98(6):1811-1818. Epub 2018 Apr 5.

Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.

Alphaviruses () are arboviruses frequently associated with emerging infectious diseases. In this study, we aimed to investigate the presence of alphaviruses in Uruguay by detecting the viral genome in mosquitoes and neutralizing antibodies in equines. A total of 3,575 mosquitoes were analyzed for alphavirus genome detection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4269/ajtmh.17-0980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086168PMC
June 2018
9 Reads

Unbiased Strain-Typing of Arbovirus Directly from Mosquitoes Using Nanopore Sequencing: A Field-forward Biosurveillance Protocol.

Sci Rep 2018 Apr 3;8(1):5417. Epub 2018 Apr 3.

MRIGlobal, 65 West Watkins Mill Road, Gaithersburg, MD, 20878, USA.

The future of infectious disease surveillance and outbreak response is trending towards smaller hand-held solutions for point-of-need pathogen detection. Here, samples of Culex cedecei mosquitoes collected in Southern Florida, USA were tested for Venezuelan Equine Encephalitis Virus (VEEV), a previously-weaponized arthropod-borne RNA-virus capable of causing acute and fatal encephalitis in animal and human hosts. A single 20-mosquito pool tested positive for VEEV by quantitative reverse transcription polymerase chain reaction (RT-qPCR) on the Biomeme two3. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-018-23641-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883038PMC
April 2018
2 Reads

Low-fidelity Venezuelan equine encephalitis virus polymerase mutants to improve live-attenuated vaccine safety and efficacy.

Virus Evol 2018 Jan 6;4(1):vey004. Epub 2018 Mar 6.

Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.

During RNA virus replication, there is the potential to incorporate mutations that affect virulence or pathogenesis. For live-attenuated vaccines, this has implications for stability, as replication may result in mutations that either restore the wild-type phenotype via reversion or compensate for the attenuating mutations by increasing virulence (pseudoreversion). Recent studies have demonstrated that altering the mutation rate of an RNA virus is an effective attenuation tool. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/ve/article/doi/10.1093/ve/vey004/49
Publisher Site
http://dx.doi.org/10.1093/ve/vey004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841381PMC
January 2018
16 Reads

Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages.

mSphere 2018 Mar-Apr;3(2). Epub 2018 Mar 21.

Molecular and Translational Sciences Division, Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.

Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), and chikungunya virus (CHIKV) are vector-borne alphaviruses that cocirculate in South America. Human infections by these viruses are frequently underdiagnosed or misdiagnosed, especially in areas with high dengue virus endemicity. Disease may progress to debilitating arthralgia (MAYV, CHIKV), encephalitis (VEEV), and death. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/mSphere.00003-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863033PMC
March 2018
6 Reads

Serological evidence of widespread exposure of Grenada fruit bats to chikungunya virus.

Zoonoses Public Health 2018 08 25;65(5):505-511. Epub 2018 Mar 25.

Pathobiology, School of Veterinary Medicine, St. George's University, St. George, Grenada.

Antibody detection against selected potentially zoonotic vector-borne alphaviruses and flaviviruses was conducted on sera from bats from all six parishes in Grenada, West Indies. Sera were tested for (i) antibodies to flaviviruses West Nile virus, St. Louis encephalitis virus, Ilhéus virus, Bussuquara virus (BSQV), Rio Bravo virus and all four serotypes of dengue virus (DENV) by plaque reduction neutralization test (PRNT); (ii) antibodies to alphaviruses western equine encephalitis virus, Venezuelan equine encephalitis virus and eastern equine encephalitis virus by epitope-blocking enzyme-linked immunosorbent assay (ELISA); and (iii) antibodies to the alphavirus chikungunya (CHIKV) by PRNT. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/zph.12460DOI Listing
August 2018
8 Reads

Development of a Broadly Accessible Venezuelan Equine Encephalitis Virus Replicon Particle Vaccine Platform.

J Virol 2018 06 14;92(11). Epub 2018 May 14.

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

Zoonotic viruses circulate as swarms in animal reservoirs and can emerge into human populations, causing epidemics that adversely affect public health. Portable, safe, and effective vaccine platforms are needed in the context of these outbreak and emergence situations. In this work, we report the generation and characterization of an alphavirus replicon vaccine platform based on a non-select agent, attenuated Venezuelan equine encephalitis (VEE) virus vaccine, strain 3526 (VRP 3526). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JVI.00027-18DOI Listing
June 2018
30 Reads

The challenges posed by equine arboviruses.

Equine Vet J 2018 Jul 14;50(4):436-445. Epub 2018 Apr 14.

School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK.

Equine populations worldwide are at increasing risk of infection by viruses transmitted by biting arthropods, including mosquitoes, biting midges (Culicoides), sandflies and ticks. These include the flaviviruses (Japanese encephalitis, West Nile and Murray Valley encephalitis), alphaviruses (eastern, western and Venezuelan encephalitis) and the orbiviruses (African horse sickness and equine encephalosis). This review provides an overview of the challenges faced in the surveillance, prevention and control of the major equine arboviruses, particularly in the context of these viruses emerging in new regions of the world. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/evj.12829DOI Listing
July 2018
4 Reads

Mosquito bottlenecks alter viral mutant swarm in a tissue and time-dependent manner with contraction and expansion of variant positions and diversity.

Virus Evol 2018 Jan 15;4(1):vey001. Epub 2018 Feb 15.

Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0610, USA.

Viral diversity is theorized to play a significant role during virus infections, particularly for arthropod-borne viruses (arboviruses) that must infect both vertebrate and invertebrate hosts. To determine how viral diversity influences mosquito infection and dissemination mosquitoes were infected with the Venezuelan equine encephalitis virus endemic strain 68U201. Bodies and legs/wings of the mosquitoes were collected individually and subjected to multi-parallel sequencing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/ve/vey001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814806PMC
January 2018
9 Reads

An unsettling explanation for the failure of skatole-baited ovitraps to capture Culex mosquitoes.

Insect Sci 2018 Feb 14. Epub 2018 Feb 14.

Department of Molecular and Cellular Biology, University of California-Davis, Davis, California, USA.

Culex mosquitoes are primarily found in temperate and tropical regions worldwide where they play a crucial role as main vectors of filarial worms and arboviruses. In Recife, a northeast city in Brazil, high densities of Culex quinquefasciatus are often found in association with human populated areas. In marked contrast to another part of the city, field tests conducted in the neighborhood of Sítio dos Pintos showed that trapping of mosquitoes in skatole-baited ovitraps did not differ significantly from captures in control (water) traps. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/1744-7917.12578DOI Listing
February 2018
4 Reads
2.140 Impact Factor