Search Our Scientific Publications & Authors

Transl Pediatr 2020 Apr;9(2):174-179

Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

() is common commensal in the female genitourinary tract. Despite has been associated with chorioamnionitis, abortion, prematurity and perinatal complications, the invasive central nervous system (CNS) infection is rare in neonates. Diagnosis of meningitis can be difficult for the atypical presentations and sterile cultures by conventional methods. Read More

J Heart Lung Transplant 2020 Apr 30;39(4S):S47. Epub 2020 Mar 30.

Infectious Diseases, Northwestern Memorial Hospital, Chicago, IL.

Purpose: Recent studies using human subjects and murine models have demonstrated a causal link infection with Ureaplasma spp in lung transplant (LTx) recipients (R) and hyperammonemia syndrome (HS). We sought to determine the source of Ureaplasma as well as the risk factors associated with the development of HS.

Methods: Consecutive patients who underwent LTxR at Northwestern Memorial Hospital from 7/2014 - 10/2018 and had Ureaplasma testing were included. Read More

BMC Infect Dis 2020 May 27;20(1):375. Epub 2020 May 27.

Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.

Background: Sexually transmitted infections (STIs) cause a major public health problem that affect both men and women in developing and developed countries. The aim of the study was to estimate the prevalence of 11 STIs among women who voluntarily participated in the study, while seeking gynecological checkup. The existence of an association between the presence of pathogens and symptoms and various sociodemographic risk factors was assessed. Read More

Eur J Clin Microbiol Infect Dis 2020 May 21. Epub 2020 May 21.

Department of Microbiology, School of Medicine, University of Granada-Instituto de Investigación Biosanitaria de Granada.ibs, Granada, Spain.

Ureaplasma parvum is the most prevalent genital mycoplasma in women of childbearing age. There is debate around the relevance of its presence in male or female genitals for disease development and as a cofactor. The objective of this study was to determine the prevalence of colonization/infection by U. Read More

Transpl Infect Dis 2020 May 19:e13332. Epub 2020 May 19.

Hartford Hospital Transplant Program, Hartford, CT, USA.

Introduction: Noncirrhotic hyperammonemia (NCH) is a rare but often fatal complication of solid organ transplantation. We present a case wherein an infectious cause of NCH was suspected following kidney transplantation (KT) and the patient was promptly started on empirical antibiotic treatment which proved to be lifesaving.

Case Report: A 56-year-old Chinese woman with a past medical history of end-stage renal disease secondary to ischemic nephropathy and cerebrovascular accident received a kidney from a 52-year-old brain-dead donor with a Kidney Donor Profile Index score of 70%. Read More

Respir Med Case Rep 2020 6;30:101080. Epub 2020 May 6.

Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Martinistr.52, D-20246, Hamburg, Germany.

Ureaplasma urealyticum is a commensal of the female genital tract and can be detected as a pathogen in urethritis and vaginitis. Its importance as a respiratory pathogen beyond the field of neonatology remains controversial. We report a case of Ureaplasma-pneumonia in a recently lung-transplanted patient, with hyperammonemic syndrome. Read More

Transpl Infect Dis 2020 May 16:e13328. Epub 2020 May 16.

Division of Infectious Diseases, The Warren Alpert Medical School of Brown University, Providence, RI, United States.

Ureaplasma species (spp.) are common colonizers of the urogenital tract but may cause systemic infection in immunocompromised patients. They release significant amounts of ammonia via urea hydrolysis and have been recently implicated in the pathogenesis of hyperammonemia syndrome after organ transplantation. Read More

BMJ Case Rep 2020 May 14;13(5). Epub 2020 May 14.

Department of Urology, Mater Hospital Brisbane, Brisbane, Queensland, Australia.

We report a case of an immunosuppressed 67-year-old woman who presented with fever of unknown origin. Further investigation revealed multiple left renal and perinephric abscesses. These were managed with percutaneous drainage and broad-spectrum antibiotics; however, no clinical improvement resulted. Read More

Parasitol Res 2020 Jun 14;119(6):1915-1923. Epub 2020 May 14.

Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.

Trichomoniasis is the most prevalent curable sexually transmitted infection (STI) worldwide and a risk factor for the acquisition of other STIs and adverse pregnancy outcomes. The objectives of this study were to determine the prevalence of T. vaginalis and related coinfections in women attending a third-level hospital of Madrid (Spain). Read More

Cancers (Basel) 2020 May 8;12(5). Epub 2020 May 8.

Institute of Virology, Department of Hygiene, Microbiology, Social Medicine, Medical University of Innsbruck, Peter-Mayr-Strasse 4b, 6020 Innsbruck, Austria.

: Certain high-risk (hr) types of human papillomavirus (HPV) can cause cervical cancer in women and penile cancer in men. Hr-HPV can also cause cancers of the oropharynx and anus in both sexes. In the anal and cervical region, a contribution of co-infections with Ureaplasma spp. Read More

Nutrients 2020 May 5;12(5). Epub 2020 May 5.

Department of Pediatrics, School for Oncology and Developmental Biology (GROW), Maastricht University, 6200 MD Maastricht, The Netherlands.

Chorioamnionitis can lead to inflammation and injury of the liver and gut, thereby predisposing patients to adverse outcomes such as necrotizing enterocolitis (NEC). In addition, intestinal bile acids (BAs) accumulation is causally linked to NEC development. Plant sterols are a promising intervention to prevent NEC development, considering their anti-inflammatory properties in the liver. Read More

J Infect Dev Ctries 2020 Apr 30;14(4):341-348. Epub 2020 Apr 30.

Department of Cell and Molecular Biology, 'Iuliu Haţieganu' University of Medicine and Pharmacy, Cluj-Napoca, Romania.

Introduction: Most sexually transmitted infections (STIs) are curable, but inappropriate treatment can lead to serious complications. The importance of setting up STI screening programs has been highlighted in various studies, the absence of such national programs accounting for the lack of STI statistics in Romania. The purpose of our study was to evaluate multiplex PCR as a screening method for the most common 6 STIs and establish their frequency in a group of symptomatic and asymptomatic patients. Read More

Front Immunol 2020 16;11:649. Epub 2020 Apr 16.

Divisions of Neonatology and Developmental Biology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA, United States.

Acute chorioamnionitis is characterized by neutrophilic infiltration and inflammation at the maternal fetal interface. It is a relatively common complication of pregnancy and can have devastating consequences including preterm labor, maternal infections, fetal infection/inflammation, fetal lung, brain, and gastrointestinal tract injury. In this review, we will discuss current understanding of the pathogenesis, immunobiology, and mechanisms of this condition. Read More

Cancers (Basel) 2020 Apr 28;12(5). Epub 2020 Apr 28.

Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

Tanzania faces one of the highest cervical cancer burdens in the world. Recent work has suggested that the bacterial family is associated with higher levels of human papillomavirus (HPV), human immunodeficiency virus (HIV), and pre-cancerous cervical lesions. infection in Tanzania is not well understood, especially when considering the differences between sexually transmitted species of . Read More

Front Immunol 2020 15;11:672. Epub 2020 Apr 15.

Department of Pediatrics, School for Oncology and Developmental Biology (GROW), Maastricht University, Maastricht, Netherlands.

[This corrects the article DOI: 10.3389/fimmu.2020. Read More

J Matern Fetal Neonatal Med 2020 Apr 29:1-9. Epub 2020 Apr 29.

Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.

Rodents are the most commonly used animals in the study of spontaneous preterm delivery (PTD). Intra-amniotic inflammation/infection is a frequent and important cause of PTD. Intraperitoneal and intrauterine administrations of inflammatory agents are traditional methods to establish a rodent model of PTD associated with inflammation and infection. Read More

Am J Obstet Gynecol 2020 Apr 25. Epub 2020 Apr 25.

Integrated Research Center for Fetal Medicine, Department of Gynecology and Obstetrics, Johns Hopkins University, School of Medicine, Baltimore, MD, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Background: Ureaplasma parvum infection is a prevalent cause of intrauterine infection that is associated with preterm birth, preterm premature rupture of membranes, the fetal inflammatory response syndrome and adverse postnatal sequelae. Elucidation of diagnostic and treatment strategies for infection-associated preterm labor may improve perinatal and long-term outcomes for these cases.

Objective: This study assesses the effect of intra-amniotic Ureaplasma infection on fetal hemodynamic and cardiac function and the impact of maternal antibiotic treatment on these outcomes. Read More

J Forensic Leg Med 2020 Apr 9;71:101935. Epub 2020 Mar 9.

Department of Laboratory Medicine, Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul, 03080, South Korea; Department of Laboratory Medicine, Seoul National University Boramae Medical Center, 20, Borama-ro 5-gil, Dongjak-gu, Seoul, 07061, South Korea. Electronic address:

Long-term observation of the prevalence of sexually transmitted infections (STIs) is unclear in sexual assault victims (SAVs).We aimed to identify the trends in the prevalence of STIs in SAVs and factors correlated with STIs. Between January 2010 and May 2019, a retrospective observational study was conducted in a regional sexual assault center in Seoul. Read More

Anaesthesist 2020 Apr 21. Epub 2020 Apr 21.

Klinik für Anästhesiologie und Intensivmedizin, Universitätsklinikum Freiburg, Hugstetter Straße 55, 79106, Freiburg, Deutschland.

A 58-year-old woman developed rapidly progressive neurological symptoms and finally loss of vigilance 5 weeks following primarily successful lung transplantation. A posterior reversible encephalopathy syndrome (PRES) under treatment with tacrolimus as well as hyperammonemia due to sepsis with Ureaplasma urealyticum could be identified as the causes. Infections with Ureaplasma, bacteria which produce ammonia as a product of metabolism, are increasingly being identified in immunocompromised people by specific PCR (polymerase chain reaction) procedures and should routinely be taken into consideration as the cause of unspecific neurological symptoms. Read More

Transpl Infect Dis 2020 Apr 19:e13298. Epub 2020 Apr 19.

Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA.

Hyperammonemia syndrome, with high levels of ammonia and neurologic dysfunction, is a syndrome with historically high mortality that may occur after solid organ transplantation. Recently, this has been associated with infection due to Ureaplasma, mostly following lung transplantation. We describe the first case of hyperammonemia syndrome due to Ureaplasma infection after liver-kidney transplantation. Read More

Fertil Steril 2020 Apr 13. Epub 2020 Apr 13.

Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy; University Vita-Salute San Raffaele, Milan, Italy. Electronic address:

Objective: To study the impact of asymptomatic semen infections on seminal parameters in men presenting for primary couple's infertility.

Design: Cross-sectional study.

Setting: Academic center. Read More

PLoS One 2020 15;15(4):e0231239. Epub 2020 Apr 15.

Eli and Edythe Broad Center of Regeneration Medicine, University of California, San Francisco, California, United States of America.

Background: Chorioamnionitis has been linked to spontaneous preterm labor and complications such as neonatal sepsis. We hypothesized that microbial cell-free (cf) DNA would be detectable in maternal plasma in patients with chorioamnionitis and could be the basis for a non-invasive method to detect fetal exposure to microorganisms.

Objective: The purpose of this study was to determine whether next generation sequencing could detect microbial cfDNA in maternal plasma in patients with chorioamnionitis. Read More

Front Immunol 2020 17;11:189. Epub 2020 Mar 17.

Department of Pediatrics, School for Oncology and Developmental Biology (GROW), Maastricht University, Maastricht, Netherlands.

Chorioamnionitis, inflammation of the fetal membranes during pregnancy, is often caused by intra-amniotic (IA) infection with single or multiple microbes. Chorioamnionitis can be either acute or chronic and is associated with adverse postnatal outcomes of the intestine, including necrotizing enterocolitis (NEC). Neonates with NEC have structural and functional damage to the intestinal mucosa and the enteric nervous system (ENS), with loss of enteric neurons and glial cells. Read More

Anticancer Res 2020 Apr;40(4):2219-2223

1 Department of Dermatology and Venereology, National and Kapodistrian University of Athens, "Andreas Sygros" Hospital for Skin and Venereal Diseases, Athens, Greece.

Aim: To investigate the prevalence of cervico-vaginal co-infection with high-risk (HR) HPV types and other sexually transmitted pathogens (STPs) in women with anogenital warts (AGWs).

Patients And Methods: In this cross-sectional study, cervico-vaginal smears of women with AGWs were examined with real-time polymerase chain reaction for the presence of HR-HPV types and common STPs. Women with recent cervical HPV infection and general population were used for comparisons. Read More

Zhonghua Nan Ke Xue 2019 Sep;25(9):802-810

Department of Urology and Andrology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.

Objective: To investigate the status quo of the diagnosis and treatment of male urethritis (MU) in urology and andrology.

Methods: According to The Guidelines for Clinical Diagnosis and Treatment of Sexually Transmitted Diseases (2017), we designed 27 questions on the prevalence, diagnosis, treatment, and prognosis of MU. Using these questions, we conducted a questionnaire investigation among urological, andrological and other relevant clinicians with different professional titles, followed by an analysis of the compliance of the doctors to the Guidelines. Read More

Turk J Obstet Gynecol 2019 Dec 28;16(4):242-248. Epub 2020 Feb 28.

Near East University Faculty of Medicine, Department of Obstetrics and Gynecology, Nicosia, Northern Cyprus.

Objective: To evaluate the prevalence of pathogens, , and , , , , and in women via multiplex-polymerase chain reaction (PCR)-deoxyribonucleic acid (DNA).

Materials And Methods: Cervical swabs of 273 women in reproductive age who underwent gynecologic examination in our outpatient clinic were evaluated using the multiplex-PCR-DNA method. The presence of cervicitis, contraceptive methods, marital status, and the number of partners were evaluated. Read More

Zhonghua Nan Ke Xue 2019 Jun;25(6):535-538

Department of Urology, Hangzhou Third People's Hospital, Hangzhou, Zhejiang 310009, China.

Objective: To investigate the infection of reproductive Mycoplasma genitalium (MG) detected by real-time fluorescence constant-temperature simultaneous amplification and testing (SAT) of nucleic acid among patients present at the clinics of urology, gynecology or venereal diseases.

Methods: We retrospectively analyzed the clinical data on 5 711 patients with suspected genitourinary tract (GUT) infection present at the clinics of urology, gynecology or sexually transmitted diseases in Hangzhou Third People's Hospital, Shaoxing People's Hospital and General Hospital of Eastern Theater Command from January 2018 to December 2018. The patients were aged 16－73 (38. Read More

Zhonghua Nan Ke Xue 2019 Feb;25(2):118-123

Department of Reproductive Epidemiology and Sociomedical Sciences / Key Laboratory of the National Health Commission for Family Planning Devices, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.

Objective: To investigate the prevalence of urogenital tract infections with Ureaplasma urealyticum (UU) and human papilloma virus (HPV) in males of reproductive age and the associated factors.

Methods: Using the multi-stage cluster sampling method and a structured questionnaire, we conducted an investigation among 18－50 years old males in Songjiang District, Shanghai, from August 2016 to July 2018. We collected secretory specimens from the urogenital tract of the subjects and detected the infections of UU and HPV by laboratory examination. Read More

Sex Transm Dis 2020 May;47(5):329-331

From the Departments of Medicine.

Identifying pathogen-specific signs or symptoms of nongonococcal urethritis could improve syndromic management accuracy. We evaluated nongonococcal urethritis signs and symptoms in 220 men with single-pathogen infections (Chlamydia trachomatis, Mycoplasma genitalium, Trichomonas vaginalis, or Ureaplasma urealyticum) or idiopathic urethritis. No individual sign or symptom accurately predicted the infectious etiology. Read More

Crit Rev Microbiol 2020 Mar 6;46(2):169-181. Epub 2020 Mar 6.

Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa.

Preterm birth is the leading cause of neonatal morbidity and mortality worldwide, and the human species are most frequently isolated from the amniotic fluid and placenta in these cases. Ureaplasma colonisation is associated with infertility, stillbirth, histologic chorioamnionitis, and neonatal morbidities, including congenital pneumonia, bronchopulmonary dysplasia, meningitis and perinatal death. The human spp. Read More

J Matern Fetal Neonatal Med 2020 Mar 4:1-6. Epub 2020 Mar 4.

National Hospital, Organization Nagasaki Medical Center, Nagasaki, Japan.

Amniotic fluid infection with or can cause chorioamnionitis and preterm birth. The aim of this study was to examine whether vaginal colonization is predictive of preterm delivery in patients exhibiting signs of threatened preterm birth or those with asymptomatic short cervix. The present retrospective study, which was performed in a perinatal tertiary center, included patients carrying a singleton pregnancy who were referred to the emergency Ob/Gyn unit because of regular preterm uterine contractions and/or short cervical length (<20 mm) at 22-33 weeks of gestation, and in whom a vaginal / examination (Urea-arginine LYO-2, BioMerieux) was performed. Read More

J Ethnopharmacol 2020 May 20;254:112611. Epub 2020 Feb 20.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address:

Ethnophamacological Relevance: The Chinese herbal prescription JieZe-1 (JZ-1) is based on the modification of Yihuang Tang, which was first described in Fu Qingzhu Nvke by the famous Qing Dynasty doctor Shan Fu as a treatment for leukorrheal diseases. As an in-hospital preparation, JZ-1 has been used in Tongji Hospital for many years to treat various infectious diseases of the lower female genital tract, including cervicitis, vaginitis, genital herpes and condyloma acuminatum. Our previous studies have shown that JZ-1 has curative effects on Candida albicans, Trichomonas vaginalis and Ureaplasma urealyticum infections. Read More

J Antimicrob Chemother 2020 Jun;75(6):1513-1517

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China and Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, 12 Urumqi Middle Road, Jing' an District, Shanghai, China.

Objectives: To assess the in vitro activities of acetylmidecamycin, a 16-membered macrolide, and 11 other antimicrobial agents against human mycoplasmas.

Methods: A total of 187 clinical isolates, Mycoplasma pneumoniae (n = 110), Mycoplasma hominis (n = 26) and Ureaplasma species (n = 51), were included in this study. The MICs of 12 antimicrobial agents, including acetylmidecamycin, thiamphenicol, chloramphenicol and some other macrolides, fluoroquinolones and tetracyclines, for these clinical isolates were determined by the broth microdilution method. Read More

J Crit Care 2020 Jun 5;57:79-83. Epub 2020 Feb 5.

Fondation Ophtalmologique Adolphe de Rothschild, Department of Neurointensive Care, Paris, France. Electronic address:

Purpose: Alert intensivists about the diagnostic pitfalls arising from hyperammonemia due to Ureaplasma infections in post-transplant patients.

Materials And Methods: Clinical observation of one patient.

Case Report: A 65-year-old female with a medical history of semi-recent kidney transplant was admitted to the Intensive Care Unit for refractory status epilepticus. Read More

J Infect Dev Ctries 2019 05 31;13(5):410-418. Epub 2019 May 31.

Laboratory of Microbiology, Research Laboratory "MPH", Habib Bourguiba University Hopsital of Sfax, Sfax University, Sfax, Tunisia.

Introduction: Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens.

Methodology: Cervicovaginal swabs and placenta samples from 132 women with miscarriage (patient group: PG), and cervicovaginal swabs from 54 women with normal pregnancy (control group:CG), were subjected to bacteriological culture and real time PCRs detecting Coxiella burnetii, Brucella spp, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Chlamydia trachomatis, Waddlia chondrophila and Parachlamydia acanthamoebeae DNA. Read More

JBRA Assist Reprod 2020 May 1;24(2):152-157. Epub 2020 May 1.

Clinical Department, Instituto Gera de Medicina Reprodutiva, São Paulo, SP, Brazil.

Objective: Most women suffering from tubal factor infertility do not have a history of pelvic inflammatory disease, but rather have asymptomatic upper genital tract infection. Investigating the impacts of such infections, even in the absence of clinically confirmed pelvic inflammatory disease, is critical to understanding the tubal factor of infertility. The aim of this study was to investigate whether the presence of endocervical bacteria is associated with tubal factors in women screened for infertility. Read More

J Infect Chemother 2020 May 29;26(5):513-515. Epub 2020 Jan 29.

Department of Infectious Diseases, University Medical Centre Ljubljana, Slovenia; Faculty of Medicine, University of Ljubljana, Slovenia.

Ureaplasma spp. usually causes genitourinary infections; few reports in the literature describe extragenital infections, usually in immunocompromised patients. We present a case of Ureaplasma parvum ventriculitis in an immunocompetent patient related to ventriculoperitoneal drainage and surgery. Read More

Open Access Maced J Med Sci 2019 Oct 14;7(20):3425-3428. Epub 2019 Oct 14.

Department of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.

Human papillomavirus (HPV) infection is one of the most prevalent sexually transmitted diseases among women aged < 35 years worldwide. Recent studies have suggested that the vaginal microenvironment influenced by bacterial infection poses for high-risk human papillomavirus (hrHPV) infection and cervical carcinogenesis. Female sex workers (FSWs) are a population susceptible to acquire Human Immunodeficiency Virus (HIV) and sexually transmitted infections (STIs), as well as transmitting the virus to others. Read More

Wiad Lek 2019 Aug;72(8):1421-1425

Kharkiv National Medical University, Kharkiv, Ukraine

Introduction: The search for a statistically significant link between sFasL and their potential regulators MMP-2 is necessary to optimize the tactics of managing and treating pregnant women with perinatal infections.

The Aim: To determine the level of MMP-2 in pregnant with perinatal infections and its role in the development of placental dysfunction.

Material And Methods: The study involved examination of 230 pregnant, who were divided into 3 groups (60 people each): Group I with viral (CMV, HSV-1,2,6 types), Group II with bacterial (chlamydia, ureaplasma, mycoplasma) and Group III with mixed infection. Read More

Infection 2020 Apr 28;48(2):259-265. Epub 2020 Jan 28.

Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität Dresden, Dresden, Germany.

Background: Rectal sexually transmitted infections (STI) are common in men having sex with men (MSM). Mycoplasma genitalium is increasingly being reported in this localization, but due to frequent lack of symptoms at this site, clinical significance is still unclear. Rectal prevalence of Mycoplasma hominis and Ureaplasma species is not well studied so far. Read More

LGBT Health 2020 Feb/Mar;7(2):119-120. Epub 2020 Jan 27.

Microbiology Department, Corporació Sanitària Parc Taulí, Universitat Autònoma de Barcelona, Barcelona, Spain.

LGBT Health 2020 Feb/Mar;7(2):121. Epub 2020 Jan 27.

Center for Health, Identity, Behavior and Prevention Studies, Rutgers School of Public Health, Rutgers University, Newark, New Jersey.

PLoS One 2020 24;15(1):e0226817. Epub 2020 Jan 24.

Microbiology, Virology and Infection Control, Great Ormond Street Hospital NHS Foundation Trust, London, United Kingdom.

Background: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture.

Methods: Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a 16S rRNA gene broad-range PCR and a multiplexed PCR for Candida spp. Read More

Postepy Biochem 2019 Oct 23;65(4):299-305. Epub 2019 Oct 23.

Zakład Diagnostyki Laboratoryjnej, Szpital Uniwersytecki im. K. Marcinkowskiego w Zielonej Górze.

Urogenital mycoplasmas belonging to Mycoplasma and Ureaplasma genera, are both commensal and pathogenic microorganisms. The gold standard for their detection in clinical samples is culture and identification based on biochemical tests. However, this method is time-consuming and not very specific. Read More

BMC Infect Dis 2020 Jan 15;20(1):47. Epub 2020 Jan 15.

University of Calgary Cumming School of Medicine, 3330 Hospital Dr NW, Calgary, AB, T2N 4N1, Canada.

Background: Ureaplasma urealyticum is a fastidious bacteria which lacks a cell wall. Extragenital infections are rare in immunocompetent adults. There are few literature reports of perinephric abscess. Read More

IET Nanobiotechnol 2020 Feb;14(1):19-24

Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

(uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target-specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide-functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of DNA. A non-cross-linking approach utilising a single Au-nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated. Read More

Nat Commun 2020 01 10;11(1):199. Epub 2020 Jan 10.

Tommy's Centre for Maternal and Fetal Health at the MRC Centre for Reproductive Health, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Cresent, Edinburgh, EH16 4TJ, UK.

Around 40% of preterm births are attributed to ascending intrauterine infection, and Ureaplasma parvum (UP) is commonly isolated in these cases. Here we present a mouse model of ascending UP infection that resembles human disease, using vaginal inoculation combined with mild cervical injury induced by a common spermicide (Nonoxynol-9, as a surrogate for any mechanism of cervical epithelial damage). We measure bacterial load in a non-invasive manner using a luciferase-expressing UP strain, and post-mortem by qPCR and bacterial titration. Read More

J Microbiol Methods 2020 Feb 3;169:105831. Epub 2020 Jan 3.

Clinical Laboratory, Minhang Hospital, Fudan University, Shanghai 201199, China. Electronic address:

Ureaplasma spp. are associated with female genital tract infections and are mainly tested by liquid culture in developing countries. To evaluate the accuracy of liquid culture, 686 vaginal swabs were collected and tested by using the Mycoplasma Culturing, Identification, Enumeration, and Susceptibility (IES) Kit. Read More

Can J Infect Dis Med Microbiol 2019 22;2019:9678104. Epub 2019 Nov 22.

Department of Clinical Laboratory, Minhang Hospital, Fudan University, No. 170 XinSong Road, Shanghai 201199, China.

Background: High-risk human papillomavirus (hr-HPV) infection is a necessary cause of cervical cancer. However, other common lower genital tract microbes may increase hr-HPV infection and their related cervical cytopathy.

Methods: To confirm this hypothesis, cervical brush and vaginal swab specimens were collected from 826 adult patients who were divided into the hr-HPV-positive group (254) and the negative group (572) by real-time PCR assay. Read More

J Obstet Gynaecol Res 2020 Mar 27;46(3):389-395. Epub 2019 Dec 27.

Department of Obstetrics and Gynecology, Center for Perinatal Medicine, University of Miyazaki, Miyazaki, Japan.

Aim: To investigate the effects of Mycoplasma/Ureaplasma cultured in amniotic fluid on perinatal characteristics in preterm delivery between 22 and 33 weeks of gestation.

Methods: The study was conducted in a tertiary perinatal center and involved 38 pregnant women who had undergone amniocentesis to evaluate intrauterine infection due to preterm labor or premature rupture of membranes. The subjects were divided into three groups based on the culture results: negative (Negative Group, n = 24), positive for Mycoplasma/Ureaplasma (M/U Group, n = 6), and positive for other pathogens (Other Pathogens Group, n = 8). Read More