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Can J Microbiol 2019 Apr 24. Epub 2019 Apr 24.

Universidade Federal da Paraiba, 28097, Molecular Biology , Cidade Universitária, Campus I , João Pessoa , Joao Pessoa, Brazil , 58051-900 ;

Ureaplasma diversum is a member of the Mollicutes class and causes urogenital tract infection in cattle and small ruminants. Studies indicate that the process of horizontal gene transfer, the exchange of genetic material among different species, has a crucial role in mollicute evolution, affecting the group's characteristic genomic reduction process and simplification of metabolic pathways. Using bioinformatics tools and the String database of known and predicted protein interactions, we constructed the protein-protein interaction network of U. Read More

J Obstet Gynaecol 2019 Apr 22:1-5. Epub 2019 Apr 22.

c Department of Obstetrics and Gynaecology , Nnamdi Azikiwe University Teaching Hospital , Nnewi , Nigeria.

Here, we aimed to investigate the predominance of cervicitis agents with minimal testing rates among asymptomatic students in a Tertiary Institution and its associated risk factors. Endocervical swabs were collected from randomly selected 133 female students (15-34 years of age) in Benin City, Nigeria and screened for six genital pathogens (Chlamydia trachomatis, Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum, U. Read More

Biomed Rep 2019 Apr 15;10(4):231-237. Epub 2019 Feb 15.

Department of Microbiology, 'Aretaieion' General Hospital, National and Kapodistrian University of Athens, Athens 11528, Greece.

Assisted reproductive techniques including fertilization (IVF) are being used increasingly worldwide and screening for genital tract infections (GTIs) is recommended prior to treatment as their presence may affect the success rate of IVF. The current study aimed to assess the possible associations between GTI-associated factors and reproductive outcome in a group of reproductive age fertile females and infertile females receiving IVF. A total of 111 infertile women enrolled in an IVF programme (Group A) and 104 fertile women (mothers of at least one child; Group B) underwent microbiological screening of vaginal and cervical samples. Read More

PLoS One 2019 10;14(4):e0214077. Epub 2019 Apr 10.

Medical Research Council: Respiratory and Meningeal Pathogens Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Background: Globally, over 400,000 neonatal deaths in 2015 were attributed to sepsis, however, the incidence and etiologies of these infections are largely unknown in low-middle income countries. We aimed to determine incidence and etiology of community-acquired early-onset (<72 hours age) sepsis (EOS) using culture and molecular diagnostics.

Methods: This was a prospective observational study, in which we conducted a surveillance for pathogens using a combination of blood culture and a polymerase chain reaction (PCR)-based test. Read More

Am J Obstet Gynecol 2019 Mar 27. Epub 2019 Mar 27.

Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea; Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam-si, Korea.

Background: Intra-amniotic infection is present in 10% of patients with an episode of preterm labor, and is a risk factor for impending preterm delivery and neonatal morbidity/mortality. Intra-amniotic inflammation is often associated with intra-amniotic infection, but sometimes is present in the absence of detectable microorganisms. Antibiotic treatment of intra-amniotic infection has traditionally been considered to be ineffective. Read More

Am J Obstet Gynecol 2019 Mar 27. Epub 2019 Mar 27.

Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. Electronic address:

Background: Cervical insufficiency is a risk factor for spontaneous midtrimester abortion or early preterm birth. Intra-amniotic infection has been reported in 8-52% of such patients, and intra-amniotic inflammation in 81%. Some professional organizations have recommended perioperative antibiotic treatment when emergency cervical cerclage is performed. Read More

Obstet Gynecol Sci 2019 Mar 13;62(2):127-133. Epub 2019 Feb 13.

Department of Obstetrics and Gynecology, Soonchunhyang University Cheonan Hospital, Soonchunhyang University College of Medicine, Cheonan, Korea.

Objective: To assess positive culture rate and antimicrobial susceptibilities of (MH) and (UU) in symptomatic general population and pregnant women admitted with preterm labor and premature rupture of membranes.

Methods: We retrospectively reviewed medical records of patients who have undergone culture test and antimicrobial susceptibilities at our center from January 2017 to April 2018. Patients with positive culture for MH, UU, or both were included in this study. Read More

Eurasian J Med 2019 Feb 30;51(1):5-7. Epub 2018 Nov 30.

Department of Clinical Microbiology and Virology, Polyclinic Tor Vergata Foundation, Rome, Italy.

Objective: To evaluate the performance of the culture colorimetric detection assay MYCO WELL D-ONE (MWD-ONE), designed to detect sexually transmitted infections using real-time polymerase chain reaction (PCR) as a reference method.

Materials And Methods: One hundred and ten urogenital samples were screened for (GV), (TV), (MH), , and (UU)/ (UP) using the MWD-ONE and real-time PCR assays species Real-TM Quant and Anyplex II STI-7 Detection, respectively.

Results: GV was detected in 33 samples by both the MWD-ONE and real-time PCR, while 6 samples gave discordant results. Read More

J Cell Physiol 2019 Mar 18. Epub 2019 Mar 18.

Institute for Maternal and Child Health-IRCCS "Burlo Garofolo", Trieste, Italy.

Recently, there are controversial opinions on the presence of Mycoplasmas/Ureaplasmas as colonizers or pathogens, and on the use of a targeted therapy. This study aimed to characterize Mycoplasmas/Ureaplasmas infections in reproductive age women, including the acquisition of sexually transmitted (ST) pathogens and poor birth outcomes. A total of 646 healthy Italian women fulfilled the inclusion criteria including 521 infertile women, 65 pregnant women, and 60 fertile women with identified risk factors and symptomatic for vaginitis/cervicitis. Read More

J Int Med Res 2019 Feb 28:300060519828945. Epub 2019 Feb 28.

1 Laboratorio de Metabolismo Experimental e Investigación Clínica; División de Investigación Clínica y Coordinación de Investigación, C.M.N. "20 de Noviembre", ISSSTE, San Lorenzo, Colonia del Valle Sur, Alcaldía Benito Juárez, C.P., Ciudad de México, México.

Objective: The present study aimed to identify Mollicutes infection in the reproductive system. We also examined the microbiological, biochemical, and antimicrobial profiles of Mollicutes infection, which are potentially associated with clinical reproductive abnormalities causing infertility in couples.

Methods: Thirty-seven couples who were attending an infertility clinic were enrolled. Read More

World J Mens Health 2019 Feb 12. Epub 2019 Feb 12.

Department of Urology, Dankook University College of Medicine, Cheonan, Korea.

Purpose: Because of the inconsistent symptoms associated with infections, their clinical significances in genitourinary tracts are under debate. Therefore, we evaluated the presence of (UU) and (UP) in urine samples and examined their associations with chronic prostatitis (CP) through a case and control study.

Materials And Methods: We included 696 nonchlamydial nongonococcal (NCNG) urine samples from men; 350 were categorized into non-inflammatory CP, 88 in inflammatory CP, and 258 in non-CP group. Read More

Ann Biol Clin (Paris) 2019 02;77(1):95-98

Laboratoire de virologie, Institut des agents infectieux (IAI) de Lyon, Centre de biologie et de pathologie Nord, Groupement hospitalier Nord, Centre hospitalier universitaire de Lyon, Lyon, France.

Mycoplasma hominis (MH) and Ureaplasma urealyticum (UU), belonging to mycoplasmas, are implicated in genital and urinary infections. These bacteria are extremely sensitive to environmental factors but remain detectable by culture methods. This study aimed to compare performance of detection on samples performed on eSwab or dry swabs. Read More

Lett Appl Microbiol 2019 May 19;68(5):409-414. Epub 2019 Mar 19.

School of Animal and Veterinary Science, The University of Adelaide, Adelaide, SA, Australia.

The aim of the study was to develop a quantitative real-time PCR assay for diagnosis and monitoring of mycoplasma urinary tract infections (UTI) in a dog. An English Cocker Spaniel dog with the history of urinary tract infection was physically examined and laboratory findings identified chronic renal insufficiency and urinary tract infection. Attempts to culture organisms from pyuric urine failed, and empirical antibiotic therapy did not resolve the pyuria. Read More

J Pediatr Hematol Oncol 2019 Feb 15. Epub 2019 Feb 15.

Miller Children's and Women's Hospital Long Beach.

Idiopathic hyperammonemia is a rare, poorly understood, and often lethal condition that has been described in immunocompromised patients. This report describes an immunocompromised patient with acute myelogenous leukemia who developed persistent hyperammonemia up to 705 µmol/L (normal, 0 to 47 µmol/L) refractory to multiple different therapies. However, after beginning azithromycin and then doxycycline therapy for Ureaplasma species infection, the patient showed immediate and sustained clinical improvement and resolution of ammonia levels. Read More

J Matern Fetal Neonatal Med 2019 Feb 20:1-9. Epub 2019 Feb 20.

e Obstetrical Intensive Care Unit, Maternal-Infant Department , Fundación Valle del Lili , Cali , Colombia.

Objective: Ureaplasma urealyticum and Mycoplasma hominis are the most common microorganisms found in the amniotic fluid of patients at risk for preterm delivery. However, culture techniques for genital mycoplasms require special conditions, are barely considered as part of the evaluation of suspected intra-amniotic infection (IAI) and the results are available within 2 and 7 days. The objectives of this study are to validate the use of two commercially available kits (Mycoplasma IES y MYCOFAST RevolutioN) for the identification of Ureaplasma spp. Read More

J Neuroinflammation 2019 Feb 14;16(1):38. Epub 2019 Feb 14.

University Children's Hospital, University of Wuerzburg, Josef-Schneider-Str. 2, 97080, Wuerzburg, Germany.

Background: Ureaplasma species (spp.) are commonly regarded as low-virulent commensals but may cause invasive diseases in immunocompromised adults and in neonates, including neonatal meningitis. The interactions of Ureaplasma spp. Read More

Reprod Sci 2019 Feb 11:1933719119828072. Epub 2019 Feb 11.

1 Department of Obstetrics and Gynecology, Division of Research, University of Kansas School of Medicine, Kansas City, KS, USA.

Objective:: Calmodulin (CaM) plays a key role in the orchestration of Ca signaling events, and its regulation is considered an important component of cellular homeostasis. The control of uterine smooth muscle function is largely dependent on the regulation of Ca and CaM signaling. The objective of this study was to investigate the expression, function, and regulation of CaM regulatory proteins in myometrium during pregnancy. Read More

Am J Obstet Gynecol 2019 Feb 5. Epub 2019 Feb 5.

Division of Neonatology, Pediatric Intensive Care and Neuropediatrics, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria.

Background: Ureaplasma species (spp) are the bacteria most often isolated from the amniotic cavity of women with preterm labor or preterm premature rupture of membranes; thus, the link between intrauterine Ureaplasma spp infection and adverse pregnancy outcome clearly is established. However, because vaginal Ureaplasma spp colonization is very common in pregnant women, the reason that these microorganisms cause ascending infections in some cases but remain asymptomatic in most pregnancies is not clear. Previous studies suggested an association between vaginal colonization with Ureaplasma parvum as opposed to U urealyticum and preterm delivery. Read More

Mikrobiyol Bul 2019 Jan;53(1):37-42

Hacettepe University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.

Laboratory testing is critical for sexually transmitted infections (STIs) as most of the infected people usually have no symptoms. It is known that having HIV and STI coinfection increases the risk of HIV transmission. Sensitive tests are required for the infection control. Read More

J Investig Clin Dent 2019 Jan 20:e12396. Epub 2019 Jan 20.

Unit of Oral Basic Investigation-UIBO, School of Dentistry, Universidad El Bosque, Bogotá, Colombia.

Aim: The aim of the present study was to establish the association between the presence of oral and uro-vaginal microorganisms in the placental membrane and preterm delivery (PTD), the premature rupture of membranes (PRM), and the clinical signs of intra-amniotic infection.

Methods: Eighty-four women with PTD and 127 women with delivery at term were assessed for the PRM, clinical signs of intra-amniotic infection, and the presence of periodontitis. Twenty-seven microorganisms were identified in the placental tissue using nested polymerase chain reaction (PCR). Read More

Antimicrob Agents Chemother 2019 Mar 26;63(3). Epub 2019 Feb 26.

Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA

Escalating levels of antibiotic resistance in mycoplasmas, particularly macrolide resistance in and , have narrowed our antibiotic arsenal. Further, mycoplasmas lack a cell wall and do not synthesize folic acid, rendering common antibiotics, such as beta-lactams, vancomycin, sulfonamides, and trimethoprim, of no value. To address this shortage, we screened nitroxoline, triclosan, and a library of 20 novel, halogenated phenazine, quinoline, and NH125 analogues against species and clinical isolates from urine. Read More

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2018 Dec;40(6):817-821

Department of Clinical Laboratory,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.

Objective To analyze the infection status of human papilloma virus (HPV),Ureaplasma urealyticum (UU),Chlamydia trachomatis (CT),and Neisseria gonorrhoeae (NG) in clinical patients.Methods The laboratory specimens including urine,urethral swabs,and cervical swabs from 870 patients from January 1st 2014 to December 31st 2017 were retrospectively analyzed. HPV-DNA was detected by multiplex fluorescent PCR,and the UU-RNA,CT-RNA,and NG-RNA were determined by isothermal nucleic acid amplification. Read More

Rev Argent Microbiol 2018 Dec 9. Epub 2018 Dec 9.

Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Córdoba, CABA, Argentina. Electronic address:

The etiology leading to neonatal damage is multifactorial, being genital infections one of the causes. The objective of the study was to identify microorganisms of the maternal genital tract that are associated with neonatal damage, in order to prevent future perinatal complications. Seven hundred and eleven pregnant patients attended their prenatal control during the period January 2010-July 2013. Read More

BMC Infect Dis 2018 Dec 12;18(1):651. Epub 2018 Dec 12.

Department of Infection Control, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, People's Republic of China.

Background: Nucleic acid amplification tests (NAAT) are well-accepted in diagnosis and surveillance of sexually infectious pathogens worldwide. However, performance differences between a RNA-based NAAT and DNA-based NAAT are rarely reported. This study compares the performances of the RNA-based SAT (simultaneous amplification and testing) assay and the DNA-based quantitative real-time polymerase chain reaction (qPCR) assay. Read More

Epidemiol Infect 2018 Dec 3:1-5. Epub 2018 Dec 3.

Department of Infection Control,Affiliated Hospital of Academy of Military Medical Sciences,Beijing,China.

The prevalence of sexually transmitted infection (STI) pathogens in Beijing, China, is rarely reported. In this study, 34 911 symptomatic outpatients with suspected genital infections who attended outpatient clinics in a tertiary care hospital were included to investigate the updated prevalence of Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and herpes simplex virus (HSV) from 1 January 2013 to 31 December 2016 in Beijing, China. Results indicated that a decrease trend (UU, CT, NG and HSV) in male and an increase trend (UU, CT and NG) in female were observed during the period. Read More

BMJ Open 2018 Nov 28;8(11):e024175. Epub 2018 Nov 28.

Public Health Interventions Research Group, Kirby Institute, University of New South Wales, Sydney, New South Wales, Australia.

Introduction: Several bacterial sexually transmitted and genital mycoplasma infections during pregnancy have been associated with poor pregnancy and perinatal outcomes. Comprehensive and systematic information about associations between sexually transmitted infections (STI) and genital infections in pregnancy and adverse perinatal outcomes is needed to improve understanding about the evidence for causal associations between these infections and adverse pregnancy and neonatal outcomes. Our primary objective is to systematically review the literature about associations between: (1) in pregnancy and preterm birth; (2) in pregnancy and preterm birth; (3) and/or in pregnancy and preterm birth. Read More

Transpl Infect Dis 2019 Apr 10;21(2):e13032. Epub 2018 Dec 10.

Division of Pediatric Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, Saint Louis, Missouri.

Ureaplasma urealyticum is a bacterial species correlated with urethritis in healthy individuals and invasive infections in immunocompromised patients. We describe a 20-year-old female with a history of remote heart transplant on everolimus, mycophenolate, and rituximab presenting with progressive urinary tract symptoms, renal failure, and neurologic symptoms. An extensive workup ultimately identified U urealyticum infection, and the patient successfully recovered after a course of azithromycin and doxycycline. Read More

Ann Clin Microbiol Antimicrob 2018 Nov 22;17(1):40. Epub 2018 Nov 22.

Institute of Clinical Microbiology, Albert Szent-Györgyi Clinical Center, Faculty of Medicine, University of Szeged, Semmelweis St. 6, Szeged, 6725, Hungary.

Objectives: The long-term use of intrauterine devices (IUDs) may lead to biofilm formation on the surface. The aim of this study was to perform the culture- and PCR-based detection of bacteria/fungi from the biofilm of the removed IUDs with different time periods in place.

Methods: For a 2-year period, 100 IUD users were involved in the study. Read More

Cancer Prev Res (Phila) 2019 Jan 21;12(1):43-56. Epub 2018 Nov 21.

Department of Gynecological Oncology, Beijing Obstetrics and Gynecology Hospital of Capital Medical University, Beijing, China.

The aim of this study was to investigate the associations between cervical microbiota and different human papillomavirus (HPV) infection statuses in cytologically normal women. The cervical microbiota of HPV-positive or -negative women with a normal cytologic diagnosis was characterized and compared using 16S rDNA-based high-throughput sequencing, and the differences in cervical microbiota associated with new acquisition, persistence, and clearances of HPV genotypes were analyzed via one-year follow-up. The results showed that the cervical microbial richness of HPV-positive women was lower than for HPV-negative women, and the difference was more significant in the postmenopausal group relative to the premenopausal group. Read More

Pediatr Int 2019 Jan;61(1):58-62

Department of Pediatrics, Hallym University Medical Center, Seoul, Korea.

Background: Ureaplasma spp. in the maternal genitourinary tract has come to attention as a cause of preterm labor, spontaneous abortion, chorioamnionitis and adverse outcomes. A few controversies, however, still remain, namely, whether it should be treated aggressively or not. Read More

Hautarzt 2018 Dec;69(12):1006-1013

Klinik und Poliklinik für Dermatologie und Venerologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Deutschland.

Background: Sexually transmitted infections (STI) are common worldwide and can cause impairment of fertility. In women pelvic inflammatory disease with subsequent tubal sterility is well-documented but the influence of STI on male fertility is still controversely discussed OBJECTIVE: The effects of various bacterial and viral STI on male fertility are discussed.

Material And Methods: An evaluation of the international literature up to 30 April 2018 was carried out including studies on larger collectives from the Asiatic region. Read More

Gut Microbes 2018 Nov 7:1-9. Epub 2018 Nov 7.

c Department of Bioinformatics and Genomics , University of North Carolina at Charlotte , Charlotte , USA.

The purpose of this prospective cross-sectional cohort pilot study is to explore the initial microbial community of gastric aspirate fluid as collected immediately after birth and its relationships with mode of delivery and preterm birth. Twenty-nine gastric aspirate samples collected immediately after birth from infants born between 24-40 weeks gestation were analyzed for microbial composition. Total microbial content was low in many samples, with a substantial number sharing taxonomic composition with negative controls. Read More

PLoS One 2018 7;13(11):e0206290. Epub 2018 Nov 7.

Inserm UMR 1153, Obstetrical, Perinatal and Pediatric Epidemiology Research Team (Epopé), Center for Epidemiology and Statistics Sorbonne Paris Cité, DHU Risks in pregnancy, Paris Descartes University, Paris, France.

Objective: To assess whether antibiotics used for treatment in asymptomatic second-trimester women positive for Mycoplasma or Ureaplasma spp. detected by amniotic-fluid PCR prevents preterm delivery.

Design: A randomized, double-blind, placebo-controlled trial. Read More

Transpl Infect Dis 2019 Feb 22;21(1):e13022. Epub 2018 Nov 22.

Division of Allergy, Pulmonary, and Critical Care, Department of Internal Medicine, University of Wisconsin Hospital and Clinics, University of Wisconsin School of Medicine-Madison School of Medicine and Public Health, Madison, Wisconsin.

Hyperammonemia, in the absence of significant liver dysfunction, is an uncommon but often fatal occurrence following orthotopic lung transplant. Prior reports have provided evidence to support Ureaplasma species as an etiology for this syndrome. This case report describes an individual post-lung transplant, treated emperically with doxycycline along with other measures to lower ammonia levels, at the time hyperammonemia with encephalopathy was recognized. Read More

BMC Infect Dis 2018 Nov 6;18(1):549. Epub 2018 Nov 6.

Laboratory Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan, 185, Ghent, Belgium.

Background: Screening of curable sexually transmitted infections is frequently oriented towards the diagnosis of chlamydia, gonorrhea, syphilis and trichomoniasis, whereas other pathogens, sometimes associated with similar urogenital syndromes, remain undiagnosed and/or untreated. Some of these pathogens are associated with adverse pregnancy outcomes.

Methods: In a nested case-control study, vaginal swabs from 79 pregnant women, i. Read More

Clin Infect Dis 2018 Oct 23. Epub 2018 Oct 23.

Department of Medicine, Section of Infectious Diseases, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA.

Background: Several studies have reported statistically significant associations of Mycoplasma genitalium with cervicitis, urethritis, or vaginal discharge, although non-significant associations have also been reported. This study examined the associations of M. genitalium with selected sexually transmitted infections (STIs), demographic, behavioral, and clinical factors among women attending a sexually transmitted disease (STD) clinic in New Orleans. Read More

Diagn Microbiol Infect Dis 2019 Mar 4;93(3):227-231. Epub 2018 Oct 4.

Femicare Clinical Research for Women, 3300, Gasthuismolenstraat 31, Tienen, Belgium; Department of Obstetrics and Gynecology, Antwerp University, 2000, Prinsstraat 13, Antwerp, Belgium.

The objective was to estimate the prevalence of Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum in healthy women and patients with altered vaginal microflora. Vaginal samples from 2594 unselected female patients were divided into normal, bacterial vaginosis (BV), and aerobic vaginitis (AV) groups and tested for U. parvum, U. Read More

Sci Rep 2018 Oct 19;8(1):15479. Epub 2018 Oct 19.

Division of Cancer Epidemiology and Management, Center for Uterine Cancer, National Cancer Center, Ilsandong-gu, Goyang, Republic of Korea.

The present study aimed to identify the cervical microbes that are associated with HPV negativity, HPV clearance and HPV persistence and to assess the microbes' longitudinal associations as related to HPV infection dynamics among Korean women. We enrolled 41 women with 107 samples, and classified them according to the HPV infection dynamics: HPV negativity (21 samples, 10 subjects), HPV clearance (42 samples, 15 subjects), and HPV persistence (44 samples, 16 subjects). Cervical swabs were collected at the baseline and six-month-interval follow-up visits. Read More

Nogyogyaszati Szuleszeti Tovabbkepzo Szemle 2018 Jun;20(3):103-112

Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U. S. Department of Health and Human Services, Bethesda, Maryland, and Detroit, Michigan, USA.

Clinical chorioamnionitis is the most common infection related diagnosis made in labor and delivery units worldwide. It is traditionally believed to be due to microbial invasion of the amniotic cavity, which elicits a maternal inflammatory response characterized by maternal fever, uterine tenderness, maternal tachycardia and leukocytosis. The condition is often associated with fetal tachycardia and a foul smelling amniotic fluid. Read More

J Med Microbiol 2018 Nov 9;67(11):1645-1654. Epub 2018 Oct 9.

2​School of Life Sciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK.

Purpose: Cervical cancer is the most frequently diagnosed female cancer in The Gambia, representing approximately 30 % of cases. In 2014, the quadrivalent human papilloma virus (HPV) vaccine was introduced, which offers protection against HPV genotypes 6, 11, 16 and 18. To evaluate the potential effectiveness of this vaccine, genotype distribution and risk factor analysis were assessed. Read More

Eur J Clin Microbiol Infect Dis 2018 Dec 22;37(12):2371-2380. Epub 2018 Sep 22.

Department of Obstetrics and Gynecology, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi, 329-0498, Japan.

Our aim was to investigate the association between vaginal Ureaplasma species (spp.) and the subsequent occurrence of chorioamnionitis (CAM), perinatal death, neonatal morbidity, and long-term neurodevelopmental impairments (NDIs) at 3 years of age. We analyzed 55 pregnant women with singleton pregnancy who had preterm premature rupture of the membranes (pPROM) at < 28 weeks of gestation, and delivered between 22 and 31 weeks at our tertiary hospital in 2007-2016. Read More

Rev Bras Ginecol Obstet 2018 Sep 19;40(9):540-546. Epub 2018 Sep 19.

Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Objective:  To determine the frequency of sexually transmitted infections (STIs) in asymptomatic women and the association of STIs with cervical intraepithelial neoplasia (CIN).

Methods:  A cross-sectional study was performed, enrolling women examined in a general gynecology clinic and in a colposcopy referral center from October 2014 to October 2015. The colposcopy group consisted of 71 women, and the general gynecology group consisted of 55 women. Read More

Zhonghua Nan Ke Xue 2018 Jun;24(6):540-546

Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.

Objective: To investigate the effects of Zhibai Dihuang Decoction (ZDD) on sperm mitochondrial permeability transition (MPT) in the rat model of ureaplasma urealyticum (UU) infection (UUI).

Methods: Ninety male SD rats were randomly divide into five groups: normal control, UUI model control, ZDD, doxycycline, and ZDD + doxycycline. The UUI model was established in the latter four groups of rats by UU injection into the bladder. Read More

Zhonghua Nan Ke Xue 2018 Jun;24(6):504-508

Zhejiang Research Institute for Family Planning Science and Technology, Hangzhou, Zhejiang 310012, China.

Objective: To investigate bacterial infection and the distribution of different bacterial species in the donor semen and the influence of different bacterial counts on semen quality.

Methods: Bacterial colonies in the semen samples from 1 126 donors were counted with the Synbiosis Protocol 3 Automatic Colony Counter and the bacterial species with a colony count ≥10⁴ cfu/ml identified with the VITEK2 Compact Automatic Biochemical Analyzer. The Makler Sperm Counting Board was used to examine the semen quality of the semen samples with a colony count = 0 cfu/ml (n = 22, group A), those with a colony count <10⁴ cfu/ml (n = 22, group B) and those with a colony count ≥10⁴ cfu/ml (n = 22, group C). Read More

Zhonghua Nan Ke Xue 2018 Feb;24(2):147-151

Center of Reproductive Medicine, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215002, China.

Objective: To investigate the prevalence of Ureaplasma urealyticum (UU) infection in infertile men, its influence on routine semen parameters and the distribution of antisperm antibody (AsAb) and its types in infertile patients with UU infection.

Methods: We detected the positive rate of UU infection, semen parameters, and the distribution of AsAb and its types in 662 infertile men and 25 normal fertile male controls followed by comparison of the obtained data between the two groups of subjects.

Results: The positive rate of UU infection was significantly higher in the infertile men than in the normal controls (52. Read More

Infect Dis Obstet Gynecol 2018 26;2018:8236575. Epub 2018 Jul 26.

Oslo University Hospital, Department of Venereology, The Olafia Clinic, Norwegian National Advisory Unit on Sexually Transmitted Infections, Oslo, Norway.

Objective: The aim of this study was to evaluate whether the polymorphonuclear leukocyte (PMNL) inflammatory response in women with nongonococcal lower genital tract infection (LGTI) can be used to optimize criteria for syndromic treatment.

Methods: A cross-sectional study of 375 women attending the STI clinic in Oslo. Urethral, cervical, and vaginal specimens underwent microscopy for PMNLs. Read More

Andrologia 2018 Dec 20;50(10):e13127. Epub 2018 Aug 20.

Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

In this study, the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvumDNA were investigated using real-time multiplex PCR detection kit in the urine samples of 50 patients who presented to the infertility polyclinic. Patients were classified into two groups in accordance with the WHO leucocytospermia criteria as over 1 × 10 /ml (n = 25) and below 1 × 10 /ml (controls; n = 25). In addition, the semen sample results of the patients were evaluated. Read More

Horm Metab Res 2018 Dec 14;50(12):932-941. Epub 2018 Aug 14.

Faculty of Life Sciences & Medicine, King's College London, London, UK.

Experimental models of hyperthyroid Graves' disease (GD) and Graves' orbitopathy (GO) are efficiently developed by genetic immunisation by electroporation with human thyrotropin hormone receptor (hTSHR) A-subunit plasmid in female BALB/c (H-2d) mice. We investigated susceptibility in C57BL/6 J (H-2b) animals to allow studies on disease mechanisms in transgenic and immune response gene knock-out mice. Higher numbers of female C57BL/6 J were positive for pathogenic thyroid stimulating antibodies, but induced hyperthyroidism remained at a low frequency compared to BALB/c animals. Read More

Antimicrob Agents Chemother 2018 Nov 24;62(11). Epub 2018 Oct 24.

Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA

Levonadifloxacin (WCK 771) was evaluated against 68 type strains and clinical isolates of , , , and spp. in comparison with moxifloxacin, levofloxacin, tetracycline, and azithromycin or clindamycin. Levonadifloxacin MICs were ≤0. Read More