269,581 results match your criteria Tuberculosis

The Mur Enzymes Chink in the Armour of Mycobacterium tuberculosis cell wall.

Eur J Med Chem 2021 Jun 2;222:113568. Epub 2021 Jun 2.

Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, District Dhule, 425405, Maharashtra, India. Electronic address:

TUBERCULOSIS: (TB) transmitted by Mycobacterium tuberculosis (Mtb) is one of the top 10 causes of death globally. Currently, the widespread occurrence of resistance toward Mtb strains is becoming a significant concern to public health. This scenario exaggerated the need for the discovery of novel targets and their inhibitors. Read More

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Rate of treatment success and associated factors in the program for drug susceptible tuberculosis in the Forest Region, Republic of Guinea, 2010-2017: a real-world retrospective observational cohort study.

Int J Infect Dis 2021 Jun 9. Epub 2021 Jun 9.

Centre Hospitalier Régional Spécialisé, Quartier Fassankoni, Commune urbaine Macenta, Région N'Zérékoré, Région Forestière, Guinée; Department of Infectious Diseases, Inselspital, Bern University Hospital, University of Bern, Switzerland.

Objectives: To analyze the treatment success rate (TSR = sum of cured or treatment completed) in the tuberculosis (TB) program for drug susceptible TB (DS-TB) of the "Centre Hospitalier Régional Spécialisé" in Macenta, Forest Region, Republic of Guinea.

Methods: This cohort study included patients starting treatment for DS-TB between 2010 and 2017. Data collection was part of the documentation for the national TB program. Read More

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HAART exacerbates testicular damage and impaired spermatogenesis in anti-Koch-treated rats via dysregulation of lactate transport and glutathione content.

Reprod Toxicol 2021 Jun 9;103:96-107. Epub 2021 Jun 9.

Reproductive Biology and Toxicology Research Laboratories, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria; Department of Morbid Anatomy, Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile-Ife, Osun State, Nigeria.

Highly active anti-retroviral therapy (HAART) is an effective anti-retroviral cocktail. Similarly, anti-Koch is highly potent against Mycobacterium tuberculosis. However, these drugs have been shown to impair male fertility. Read More

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Population Pharmacokinetics of Oral Levofloxacin in Healthy Volunteers and Dosing Optimization for Multidrug-resistant Tuberculosis Therapy.

Biopharm Drug Dispos 2021 Jun 11. Epub 2021 Jun 11.

Division of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkla, 90110, Thailand.

Background: Levofloxacin is considered to be a key component of multidrug-resistant tuberculosis (MDR-TB) regimen. However, there is considerable concern regarding the subtherapeutic concentrations of currently used doses and the development of drug resistance.Therefore, this study aimed to describe the population pharmacokinetics of oral levofloxacin in healthy volunteers and to evaluate the probability of target attainment (PTA) in an attempt to optimize the dosing regimens for MDR-TB therapy. Read More

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Cholesterol-dependent transcriptome remodeling reveals new insight into the contribution of cholesterol to Mycobacterium tuberculosis pathogenesis.

Sci Rep 2021 Jun 11;11(1):12396. Epub 2021 Jun 11.

Laboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of Sciences, Łódź, Poland.

Mycobacterium tuberculosis (Mtb) is an obligate human pathogen that can adapt to the various nutrients available during its life cycle. However, in the nutritionally stringent environment of the macrophage phagolysosome, Mtb relies mainly on cholesterol. In previous studies, we demonstrated that Mtb can accumulate and utilize cholesterol as the sole carbon source. Read More

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Reinvestigation of the structure-activity relationships of isoniazid.

Tuberculosis (Edinb) 2021 Jun 5;129:102100. Epub 2021 Jun 5.

Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN, 55455, USA. Electronic address:

Isoniazid (INH) remains a cornerstone for treatment of drug susceptible tuberculosis (TB), yet the quantitative structure-activity relationships for INH are not well documented in the literature. In this paper, we have evaluated a systematic series of INH analogs against contemporary Mycobacterium tuberculosis strains from different lineages and a few non-tuberculous mycobacteria (NTM). Deletion of the pyridyl nitrogen atom, isomerization of the pyridine nitrogen to other positions, replacement of the pyridine ring with isosteric heterocycles, and modification of the hydrazide moiety of INH abolishes antitubercular activity. Read More

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ADP-ribosylation of DNA and RNA.

DNA Repair (Amst) 2021 Jun 3;105:103144. Epub 2021 Jun 3.

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. Electronic address:

ADP-ribosylation is a chemical modification of macromolecules found across all domains of life and known to regulate a variety of cellular processes. Notably, it has a well-established role in the DNA damage response. While it was historically known as a post-translational modification of proteins, recent studies have shown that nucleic acids can also serve as substrates of reversible ADP-ribosylation. Read More

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Synthesis and structure-activity relationship of new chalcone linked 5-phenyl-3-isoxazolecarboxylic acid methyl esters potentially active against drug resistant Mycobacterium tuberculosis.

Eur J Med Chem 2021 Jun 2;222:113580. Epub 2021 Jun 2.

Department of Pharmaceutical Technology and Process Chemistry, National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, 500037, Telangana, India. Electronic address:

In search of novel therapeutic agents active against emerging drug-resistant Mycobacterium tuberculosis and to counter the long treatment protocol of existing drugs, herein we present synthesis and biological evaluation of a new series of 5-phenyl-3-isoxazolecarboxylic acid methyl ester-chalcone hybrids. Among 35 synthesized compounds, 32 analogues displayed potent in-vitro activity against Mycobacterium tuberculosis H37Rv with MIC 0.12-16 μg/mL. Read More

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Development and validation of signature sequence-based PCR for improved molecular diagnosis of tuberculosis.

J Mol Diagn 2021 Jun 8. Epub 2021 Jun 8.

ICMR-National Institute of Pathology, Safdarjung Hospital Campus, New Delhi. Electronic address:

Reliable, fast, and affordable diagnosis for TB remains a challenge to reduce disease incidence in resource-poor countries. Tests based on nucleotide sequences that are 'signature' to M. tuberculosis (M. Read More

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Tuberculosis of the spine in children - Does drug resistance affect surgical outcomes?

Spine J 2021 Jun 8. Epub 2021 Jun 8.

Paediatric TB Clinic, Bai Jerbai Wadia Hospital for Children, Mumbai, India. Electronic address:

Background Context: The emergence of drug resistance has complicated the management of spinal tuberculosis (TB). While it is well known that the medical management of drug-resistant spinal TB is more difficult, the surgical outcomes of the same have not been studied sufficiently, particularly in children.

Purpose: To analyze the surgical outcomes in a cohort of children treated for spinal TB, and to thus assess whether drug resistant (DR) disease is associated with poorer surgical outcomes. Read More

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Role of chitosan based nanomedicines in the treatment of chronic respiratory diseases.

Int J Biol Macromol 2021 Jun 8. Epub 2021 Jun 8.

Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak 124001, India. Electronic address:

Chitosan-loaded nanomedicines provide a greater opportunity for the treatment of respiratory diseases. Natural biopolymer chitosan and its derivatives have a large number of proven pharmacological actions like antioxidant, wound healing, immuno-stimulant, hypocholesterolemic, antimicrobial, obesity treatment, anti-inflammatory, anticancer, bone tissue engineering, antifungal, regenerative medicine, anti-diabetic and mucosal adjuvant, etc. which attracted its use in the pharmaceutical industry. Read More

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Prevalence of active tuberculosis disease among healthcare workers and support staff in healthcare settings of the Amhara region, Ethiopia.

PLoS One 2021 11;16(6):e0253177. Epub 2021 Jun 11.

KNCV Tuberculosis Foundation, The Hague, The Netherlands.

Background: Health care workers (HCWs) are at an increased risk of acquiring tuberculosis (TB) compared to the general population, because of the frequent face to face contact or potential exposure to TB through shared air or space with infectious patient(s), regardless of economic setting and local TB incidence. Information on the burden of active TB disease among HCWs will help guide control measures, can be utilized to evaluate the effectiveness of TB infection prevention programs in the health care setting, and guide necessary actions. However, data on TB among HCW in Ethiopia is limited. Read More

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High rate of completion for weekly rifapentine plus isoniazid treatment in Chinese children with latent tuberculosis infection-A single center study.

PLoS One 2021 11;16(6):e0253159. Epub 2021 Jun 11.

Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Three months of weekly rifapentine plus isoniazid (3HP) is a short course regimen for latent tuberculosis infection treatment with satisfied safety and efficacy. However, research on its use in children is limited. In this study, we evaluated the completion rate and safety of the 3HP regimen among children in China. Read More

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Rapid detection of multidrug-resistant tuberculosis based on allele-specific recombinase polymerase amplification and colorimetric detection.

PLoS One 2021 11;16(6):e0253235. Epub 2021 Jun 11.

Research Unit of Innovative Diagnosis of Antimicrobial Resistance, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to TB control. Early diagnosis and proper treatment are essential factors to limit the spread of the disease. The existing molecular tests for MDR-TB usually require specific instruments, steady power supply, and routine maintenance, which might be obstacles for low-resource settings. Read More

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Impact of vitamin D status and cathelicidin antimicrobial peptide on adults with active pulmonary TB globally: A systematic review and meta-analysis.

PLoS One 2021 11;16(6):e0252762. Epub 2021 Jun 11.

Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University Kampala, Uganda.

Background: Tuberculosis remains a global threat and a public health problem that has eluded attempts to eradicate it. Low vitamin D levels have been identified as a risk factor for tuberculosis infection and disease. The human cathelicidin LL-37 has both antimicrobial and immunomodulatory properties and is dependent on vitamin D status. Read More

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Effects of pain on depression, sleep, exercise tolerance, and quality of life in patients with nontuberculous mycobacterial pulmonary disease.

Medicine (Baltimore) 2021 Jun;100(23):e26249

Department of Clinical Mycobacteriology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki.

Abstract: The experience and causes of pain in patients with nontuberculous mycobacterial pulmonary disease (NTM-PD) have not been clarified.This study aimed to determine the prevalence and severity of bodily pain (BP) in patients with NTM-PD. We also investigated the clinical indicators that contribute to pain. Read More

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Risk factors associated with postoperative respiratory failure in tuberculous empyema patients.

Medicine (Baltimore) 2021 Jun;100(23):e25754

Department of Thoracic Surgery.

Abstract: Our objective was to identify independent risk factors for predicting which patients in the Chinese population would likely develop respiratory failure.A descriptive analysis was conducted of demographic and clinical data of patients with tuberculous empyema (TE) admitted to the Beijing Chest Hospital, Capital Medical University between January 2001 and January 2020. Risk factors associated with postsurgical respiratory failure in TE patients were identified based on results of analyses based on univariable and multivariable logistic regression models. Read More

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Attitudes, beliefs and practice of Egyptian healthcare workers towards seasonal influenza vaccination.

Influenza Other Respir Viruses 2021 Jun 11. Epub 2021 Jun 11.

World Health Organization Country Office, Cairo, Egypt.

Background: Seasonal influenza vaccination is highly recommended for healthcare workers (HCWs) every year to protect them and reduce the risk of disease transmission at workplaces. Relatively few studies addressed influenza vaccination in the Eastern Mediterranean Region.

Objectives: The main objective of this study was to explore the attitudes, beliefs and practice of Egyptian HCWs towards seasonal influenza vaccine. Read More

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Apoptosis assessment in high-content and high-throughput screening assays.

Biotechniques 2021 Jun 11;70(6):309-318. Epub 2021 Jun 11.

Department of Medicine, Life Sciences Institute, University of British Columbia, Vancouver, Canada.

Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays. AUTOptosis is based on the quantification of nuclei intensity via staining with Hoechst 33342. First, the authors calibrated the method using standard apoptosis inducers in multiple cell lines. Read More

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"One-two Punch": Synergistic β-lactam Combinations for Mycobacterium abscessus and Target Redundancy in the Inhibition of Peptidoglycan Synthesis Enzymes.

Clin Infect Dis 2021 Jun 11. Epub 2021 Jun 11.

Department of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, OH, USA.

Mycobacterium abscessus subsp. abscessus is one of the most difficult pathogens to treat and its incidence in disease is increasing. Dual β-lactam combinations act synergistically in vitro, but are not widely employed in practice. Read More

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Diagnostic approach to a cardiac mass: a case report of misdiagnosed cardiac synovial sarcoma.

Eur Heart J Case Rep 2021 Mar 10;5(3):ytab039. Epub 2021 Mar 10.

Department of Cardiology, University Hospital of Ibn Rochd, Casablanca , Morocco.

Background: Cardiac synovial sarcoma (CSS) is an extremely rare malignant tumour with a poor prognosis. We report the case of a 31-year-old woman who presented with a CSS in the right atrium and was initially misdiagnosed with a tuberculoma. The aim of this article is to focus on the importance of having broad differential diagnoses including rare entities. Read More

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Latent Tuberculosis Infection Status of Pregnant Women in Uganda Determined Using QuantiFERON TB Gold-Plus.

Open Forum Infect Dis 2021 Jun 11;8(6):ofab241. Epub 2021 May 11.

Department of Medicine, School of Medicine, Makerere University College of Health Sciences, Kampala, Uganda.

Background: The risk of progression of latent tuberculosis infection (LTBI) to active disease increases with pregnancy. This study determined the prevalence and risk factors associated with LTBI among pregnant women in Uganda.

Methods: We enrolled 261 pregnant women, irrespective of gestational age. Read More

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Novel Long Non-coding RNA and LASSO Prediction Model to Better Identify Pulmonary Tuberculosis: A Case-Control Study in China.

Front Mol Biosci 2021 25;8:632185. Epub 2021 May 25.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Introduction: The insufficient understanding and misdiagnosis of clinically diagnosed pulmonary tuberculosis (PTB) without an aetiological evidence is a major problem in the diagnosis of tuberculosis (TB). This study aims to confirm the value of Long non-coding RNA (lncRNA) n344917 in the diagnosis of PTB and construct a rapid, accurate, and universal prediction model.

Methods: A total of 536 patients were prospectively and consecutively recruited, including clinically diagnosed PTB, PTB with an aetiological evidence and non-TB disease controls, who were admitted to West China hospital from Dec 2014 to Dec 2017. Read More

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Middle Lobe Syndrome Associated with Bronchial Anthracofibrosis.

Acta Med Litu 2020 21;27(2):100-103. Epub 2020 Dec 21.

Canakkale Onsekiz Mart University, School of Medicine, Department of Radiology, 17100, Canakkale, Turkey Orcid id: 0000-0002-9296-9489.

Bronchial anthracofibrosis has been defined as airway narrowing associated with dark pigmentation on bronchoscopy without an appropriate history of pneumoconiosis or smoking. We present a case of a 67-year-old, non-smoking female patient who was referred to our clinic for two years of persistent cough. Spirometry was within normal limits. Read More

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December 2020

Persistent Discrimination of TB in Southeastern China: Results from Four Repeated Population-Based Surveys During the Period of 2006-2018.

Risk Manag Healthc Policy 2021 3;14:2333-2344. Epub 2021 Jun 3.

Department of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.

Objective: To analyze the changes in discriminatory attitudes and isolated behaviors of the public toward tuberculosis (TB) in the Zhejiang Province and to determine the associated factors with TB discrimination.

Methods: Data were obtained from four cross-sectional population-based investigations from 2006 to 2018. A total of 26,246 respondents were interviewed using unified questionnaires that measured knowledge, attitudes, and behaviors regarding TB. Read More

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NAT2 Gene rs1041983 is Associated with Anti-Tuberculosis Drug Induced Hepatotoxicity Among Pediatric Tuberculosis in Bandung, Indonesia.

Appl Clin Genet 2021 3;14:297-303. Epub 2021 Jun 3.

Department of Child Health.

Background: As pediatric tuberculosis (TB) globally is still reported challenging in diagnosis, to date, a lot of efforts have been established to eliminate the disease including proper treatment regimen using anti-TB drugs. However, antituberculosis drug-induced hepatotoxicity (ADIH) is known to interfere the success of the prescribed therapy. ADIH was found to be correlated with polymorphisms of NAT2 gene, that is responsible to transcript the NAT2 enzyme, a metabolizer of isoniazid (INH). Read More

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Trends of Drug Resistance Tuberculosis from 2014 to 2018, Bale Zone, Oromia Region, Ethiopia.

Infect Drug Resist 2021 3;14:2073-2078. Epub 2021 Jun 3.

Negelle Borena Health Science College, Negelle Borena, Oromia Regional State, Ethiopia.

Purpose: Multidrug-resistant tuberculosis threatens global tuberculosis care and prevention and remains a major public health concern in many countries. In 2016, there were an estimated 490,000 cases of MDR and 110,000 more cases resistant to rifampicin (RR TB). Ethiopia is among the highest MDR TB burden countries according to the WHO. Read More

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High-dose Mycobacterium tuberculosis aerosol challenge cannot overcome BCG-induced protection in Chinese origin cynomolgus macaques; implications of natural resistance for vaccine evaluation.

Sci Rep 2021 Jun 10;11(1):12274. Epub 2021 Jun 10.

National Infection Service, Public Health England, Porton Down, Wiltshire, SP4 0JG, UK.

This study describes the use of cynomolgus macaques of Chinese origin (CCM) to evaluate the efficacy and immunogenicity of the BCG vaccine against high dose aerosol Mycobacterium tuberculosis challenge. Progressive disease developed in three of the unvaccinated animals within 10 weeks of challenge, whereas all six vaccinated animals controlled disease for 26 weeks. Three unvaccinated animals limited disease progression, highlighting the intrinsic ability of this macaque species to control disease in comparison to macaques of other species and genotypes. Read More

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Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis.

Sci Rep 2021 Jun 10;11(1):12267. Epub 2021 Jun 10.

Laboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.

Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. Read More

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The burden of drug resistance tuberculosis in Ghana; results of the First National Survey.

PLoS One 2021 10;16(6):e0252819. Epub 2021 Jun 10.

Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Resistance to Tuberculosis drugs has become a major threat to the control of tuberculosis (TB) globally. We conducted the first nation-wide drug resistance survey to investigate the level and pattern of resistance to first-line TB drugs among newly and previously treated sputum smear-positive TB cases. We also evaluated associations between potential risk factors and TB drug resistance. Read More

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