248,704 results match your criteria Tuberculosis


Inhibition mechanism of L,D-transpeptidase 5 in presence of the β-lactams using ONIOM method.

J Mol Graph Model 2018 Nov 28;87:204-210. Epub 2018 Nov 28.

Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa. Electronic address:

Tuberculosis (TB) is one of the world's deadliest diseases resulting from infection by the bacterium, Mycobacterium tuberculosis (M.tb). The L,D-transpeptidase enzymes catalyze the synthesis of 3 → 3 transpeptide linkages which are predominant in the peptidoglycan of the M. Read More

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November 2018
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Computed tomography thorax abnormalities in immunocompetent patients with tuberculous meningitis: An observational study.

J Neurol Sci 2018 Dec 7;397:11-15. Epub 2018 Dec 7.

Department of Neurology, King George Medical University, Lucknow, Uttar Pradesh, India.

Objective: Computed tomography (CT) of the thorax is the imaging modality of choice to detect or demonstrate lesions suggestive of active pulmonary tuberculosis. We aimed to evaluate the imaging abnormalities detected on CT of the thorax in patients with tuberculous meningitis (TBM).

Methods: In this prospective study, we enrolled consecutive newly-diagnosed patients with TBM. Read More

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December 2018

Accuracy of IP-10 for diagnosing latent tuberculosis infection: a systematic review and meta-analysis.

Clin Microbiol Infect 2018 Dec 13. Epub 2018 Dec 13.

Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan University, Chengdu, China. Electronic address:

Background: Effective diagnostic methods for detecting latent tuberculosis infection (LTBI) are important for its eradication. A number of studies have evaluated the use of interferon gamma-induced protein 10 (IP-10), which is elevated after tuberculosis infection, as a biomarker for LTBI, but conclusive results regarding its effectiveness have not been reported.

Objectives: Our objective was to assess the diagnostic value of IP-10 for LTBI. Read More

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December 2018

A comprehensive analysis of the protein-ligand interactions in crystal structures of Mycobacterium tuberculosis EthR.

Biochim Biophys Acta Proteins Proteom 2018 Dec 13. Epub 2018 Dec 13.

Unité Microbiologie, Bioorganique et Macromoléculaire (CP206/04), département R3D, Faculté de Pharmacie, Université Libre de Bruxelles, B-1050 Brussels, Belgium. Electronic address:

The Mycobacterium tuberculosis EthR is a member of the TetR family of repressors, controlling the expression of EthA, a mono-oxygenase responsible for the bioactivation of the prodrug ethionamide. This protein was established as a promising therapeutic target against tuberculosis, allowing, when inhibited by a drug-like molecule, to boost the action of ethionamide. Dozens of EthR crystal structures have been solved in complex with ligands. Read More

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December 2018

Rapid synthesis and immunogenicity of mycobacterial (1→5)-α-d-arabinofuranan.

Carbohydr Polym 2019 Feb 24;206:262-272. Epub 2018 Oct 24.

School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, 12121, Thailand. Electronic address:

A rapid synthesis of the α(1→5) arabinofuranan polysaccharides, found on the outer surface of Mycobacterium tuberculosis (Mtb), is achieved by a regio- and stereocontrolled ring opening polymerization of β-d-arabinofuranose-1,2,5-orthobenzoate. The robust polymerization reaction allows the incorporation of an amine linker, which was used to conjugate with protein tetanus toxoid (TT) to further investigate its adjuvant activities. The synthetic arabinan, which is the glycan on the non-reducing end of Mtb lipoarabinomannan (LAM), was evaluated for its immunological properties in vitro and in vivo. Read More

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February 2019
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Functionalized rifampicin-loaded nanostructured lipid carriers enhance macrophages uptake and antimycobacterial activity.

Colloids Surf B Biointerfaces 2018 Dec 4;175:306-313. Epub 2018 Dec 4.

Laboratório de Fitotecnologia, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Brazil. Electronic address:

Tuberculosis is an infectious bacterial disease that causes millions of deaths worldwide. Current treatment recommended by WHO is effective, however it is an extensive and arduous process associated to severe adverse effects, which induces a low patient compliance and the emerging of multidrug resistant tuberculosis. Thus, as a main goal of this study, rifampicin nanoparticles were surface functionalized with a tuftsin-modifed peptide to selectively recognize receptors located on infected alveolar macrophages, enhancing nanoparticles uptake by these cells and improving antimycobacterial activity. Read More

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December 2018
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Genetic susceptibility to Tuberculosis: Interaction between HLA-DQA1 and age of onset.

Infect Genet Evol 2018 Dec 12. Epub 2018 Dec 12.

Department of Microbiology, The Chinese University of Hong Kong, Hong Kong, China.

Several genome-wide association studies (GWAS) identified new single nucleotide polymorphisms (SNPs) with susceptibility to Tuberculosis (TB). However, many of them were not replicated across ethnic groups. The cause of this phenomenon of genetic heterogeneity is uncertain. Read More

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December 2018
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Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis.

Immunol Res 2018 Dec 14. Epub 2018 Dec 14.

FGBU "St. Petersburg Scientific Research Institute of Phthisiopulmonology" of the Ministry of Health of Russia, St. Petersburg, Russia.

Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data on the etiology of the disease. The exclusion of tuberculosis in every patient with suspected sarcoidosis is a mandatory stage of diagnosis, especially in countries with a high burden of tuberculosis. Read More

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December 2018
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Hif-1α-Induced Expression of Il-1β Protects against Mycobacterial Infection in Zebrafish.

J Immunol 2018 Dec 14. Epub 2018 Dec 14.

The Bateson Centre, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom;

Drug-resistant mycobacteria are a rising problem worldwide. There is an urgent need to understand the immune response to tuberculosis to identify host targets that, if targeted therapeutically, could be used to tackle these currently untreatable infections. In this study we use an Il-1β fluorescent transgenic line to show that there is an early innate immune proinflammatory response to well-established zebrafish models of inflammation and infection. Read More

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December 2018
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Synthesis, biological evaluation and molecular docking study of 1,2,3-1H-triazoles having 4H-pyrano[2,3-d]pyrimidine as potential Mycobacterium tuberculosis protein tyrosine phosphatase B inhibitors.

Bioorg Med Chem Lett 2018 Dec 6. Epub 2018 Dec 6.

Faculty of Chemistry, VNU University of Science, 19 Le Thanh Tong, Ha Noi, Viet Nam; Thai Nguyen College of Education, Quang Trung, Thinh Dan, Thai Nguyen, Viet Nam.

Some heterocycles, namely 2-amino-4H-pyran-3-carbonitriles, were synthesized in a three-component reaction from substituted benzaldehydes, malononitrile, and ethyl acetoacetate. These heterocycles have been converted subsequently into 4H-pyrano[2,3-d]pyrimidine ring by ring-closing reaction with acetic anhydride in the presence of the concentrated sulfuric acid as catalyst. The successive alkylation reaction of lactam NH bond on pyrimidine-4-one ring was carried out using propargylic bromide in dry acetone in the presence of anhydrous potassium carbonate. Read More

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December 2018

A simple and rapid-acting approach for the reduction of C-reactive protein.

Biomed Pharmacother 2019 Jan 29;109:2305-2308. Epub 2018 Nov 29.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

C-reactive protein (CRP) is an acute-phase protein which can bind to and aggregate oxidized low-density lipoprotein (ox-LDL) particles, thereby enhancing the uptake of oxLDL by macrophages. This finally leads to the formation of foam cells that are a typical characteristic of atherosclerotic plaques. Serum CRP has been shown to bind to phospholipids such as phophatidylcholine (PC), phosphatidylglycerol (PG) and phosphatidylserine (PS). Read More

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January 2019

Pharmacologic ascorbate as a pro-drug for hydrogen peroxide release to kill mycobacteria.

Biomed Pharmacother 2019 Jan 27;109:2119-2127. Epub 2018 Nov 27.

Scientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Jinshan District, Shanghai, China. Electronic address:

Background And Purpose: Tuberculosis is one of the most highly fatal diseases worldwide, and one-third of the world's population has been infected with Mycobacterium tuberculosis (M. tuberculosis). A previous study showed that M. Read More

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January 2019
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Inhibition of TREM1 reduces inflammation and oxidative stress after spinal cord injury (SCI) associated with HO-1 expressions.

Biomed Pharmacother 2019 Jan 26;109:2014-2021. Epub 2018 Nov 26.

Orthopedics Department, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Pudong, Shanghai, 201399, China. Electronic address:

Spinal cord injury (SCI) is a devastating event, leading to the progression of chronic neuropathic pain syndrome. Triggering receptor expressed on myeloid cells 1 (TREM1) is an innate immune receptor expressed on neutrophils and monocytes/macrophages. TREM1 enhances inflammatory response in various models of diseases, but its significance in SCI remains unclear. Read More

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January 2019
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Etiology and epidemiology of community-acquired pneumonia in adults requiring hospital admission: a prospective study in rural Central Philippines.

Int J Infect Dis 2018 Dec 11. Epub 2018 Dec 11.

Department of Virology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Japan980-8575; Collaborating Research Center for Emerging and Reemerging Infectious Diseases, 9002 Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines 1781.

Background: Community-acquired pneumonia (CAP) is a common cause of morbidity and mortality among adults worldwide. However, the distribution of etiology of CAP varies from one country to another with limited data from rural areas.

Methods: A prospective hospital-based study on adult CAP was conducted in Leyte, Central Philippines from May 2010 to May 2012. Read More

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December 2018
2.330 Impact Factor

A role for Wnt/β-catenin signalling in suppressing Bacillus Calmette-Guerin-induced macrophage autophagy.

Microb Pathog 2018 Dec 11. Epub 2018 Dec 11.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, Yinchuan, 750021, Ningxia, China; College of Life Science, Ningxia University, Yinchuan, 750021, Ningxia, China. Electronic address:

Mycobacterium tuberculosis (Mtb)-induced autophagy of alveolar macrophages has been confirmed to play a central role in the pathogenesis of tuberculosis. Growing evidence indicates that excessive or uncontrolled autophagic activity, which results in type II programmed cell death, can be regulated by many factors, including Wnt/β-catenin signalling. Wnt/β-catenin signalling has been demonstrated to be involved in multiple diseases through the regulation of autophagy; however, its exact role in regulating autophagy induced by Mtb remains unclear. Read More

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December 2018
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A structural and functional perspective on the enzymes of Mycobacterium tuberculosis involved in the L-rhamnose biosynthesis pathway.

Prog Biophys Mol Biol 2018 Dec 11. Epub 2018 Dec 11.

School of Chemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India. Electronic address:

Tuberculosis is one of the leading causes of death from bacterial infections. The multi-drugresistant strain has warranted the development of new drug molecules which can inhibit the growth of Mycobacterium tuberculosis. Most of the known drugs inhibit the enzymes in the cell wall biosynthesis. Read More

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December 2018

Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria.

PLoS One 2018 14;13(12):e0208940. Epub 2018 Dec 14.

University of Washington, Seattle, WA, United States of America.

Rationale: HSP90B1, also known as gp96, is a chaperone for multiple Toll-like receptors (TLRs) and is necessary for TLR-mediated inflammatory responses in murine myeloid cells. The molecule is also expressed in T-cells though its specific role is unknown. We hypothesized that human HSP90B1 regulates monocyte and T-cell responses to Mycobacterium tuberculosis (Mtb) and bacilli Calmette-Guerin (BCG) and that its variants are associated with susceptibility to TB disease. Read More

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December 2018
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Acceptability of a Novel Levofloxacin Dispersible Tablet Formulation in Young Children Exposed to Multidrug-Resistant Tuberculosis.

Pediatr Infect Dis J 2018 Dec 13. Epub 2018 Dec 13.

Desmond Tutu TB Centre, Department of Paediatrics and Child Health, Faculty of Medicine and Child Health, Stellenbosch University, Cape Town.

Levofloxacin is used for the treatment and prevention of multidrug-resistant tuberculosis in children, but current adult formulations are poorly palatable. A questionnaire administered to caregivers of 27 children taking a novel 100 mg dispersible taste-masked levofloxacin tablet found the new formulation to be more palatable (69%) and easier to prepare (81%) than the adult formulation. This formulation may assist children to better adhere to anti-tuberculous therapy. Read More

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December 2018

Drug Susceptibility of Non-tuberculous Strains of Isolated from Birds from Poland.

Pol J Microbiol 2018 ;67(4):487-492

Department of Pathology and Veterinary Diagnostics, Warsaw University of Life Sciences , Warsaw , Poland.

Mycobacterioses are a constant problem in backyard poultry, as well as pet birds. To date, no evidence of direct transmission of atypical bacilli between humans has been demonstrated, but it cannot be ruled out that sick animals can be a source of infection for people in their environment. The aim of the study was to identify mycobacteria isolated from birds with diagnosed mycobacteriosis and to determine the susceptibility of mycobacterial isolates from these animals to antituberculous drugs most commonly used in the treatment of mycobacterial infections in humans. Read More

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January 2018
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Serum Cryptococcal Antigen Confirmed Pulmonary Cryptococcosis in an Immune-competent Adult Firstly Misdiagnosed of Tuberculosis from a Lung Biopsy: a Case Report and Literature Review.

Clin Lab 2018 Oct;64(11)

Background: Cryptococcal pneumonia is an uncommon lesion in immune-competent adults. Histological evidence of Cryptococcus neoformans is a gold criterion for diagnosis. Here we report a case firstly misdiagnosed as tuberculosis from a lung biopsy. Read More

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October 2018
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Use of network analysis multidrug-resistant tuberculosis contact investigation in Kanchanaburi, Thailand.

Trop Med Int Health 2018 Dec 14. Epub 2018 Dec 14.

Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.

Objective: To characterize an MDR-TB outbreak and incorporate social network analysis with contact investigation to detect case-contact linkages and clusters.

Methods: MDR-TB cases registered in the district hospital between October 2012 and September 2015 were interviewed and their contacts were investigated. A relationship-based weighted network was constructed. Read More

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December 2018
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Immunotherapeutic effects of recombinant adenovirus encoding interleukin 12 in experimental pulmonary tuberculosis.

Scand J Immunol 2018 Dec 13:e12743. Epub 2018 Dec 13.

Sección de Patología Experimental, Departamento de Patología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran, México.

High dose of Mycobacterium tuberculosis (Mtb) strain H37Rv administered by intratracheal injection in BALB/c mice induce progressive tuberculosis (TB). In this model during the first month there is a temporal control of bacillary growth, in co-existence with macrophage activation, granuloma formation and Th-1 response. Then, bacterial proliferation recommences, accompanied by progressive pneumonia and decreasing expression of protective cytokines (IFN-γ, TNF-α). Read More

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December 2018

PREDICTION ALGORITHM OF THE CAT SPINAL SEGMENTS LENGTHS AND POSITIONS IN RELATION TO THE VERTEBRAE.

Anat Rec (Hoboken) 2018 Dec 12. Epub 2018 Dec 12.

Pavlov Institute of Physiology, Russian Academy of Sciences, nab. Makarova 6, Saint-Petersburg, 199034, Russia.

Detailed knowledge of the topographic organization and precise access to the spinal cord segments is crucial for the neurosurgical manipulations as well as in vivo neurophysiological investigations of the spinal networks involved in sensorimotor and visceral functions. Because of high individual variability, accurate identification of particular portion of the lumbosacral enlargement is normally possible only during postmortem dissection. Yet, it is often necessary to determine the precise location of spinal segments prior to in vivo investigation, targeting spinal cord manipulations, neurointerface implantations, and neuronal activity recordings. Read More

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December 2018
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Bedaquiline and delamanid in the treatment of multidrug-resistant tuberculosis: Promising but challenging.

Drug Dev Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.

Hit, Lead & Candidate Discovery Improving treatment outcomes in multidrug-resistant tuberculosis (MDR-TB) is partly hampered by inadequate effective antitubercular agents. Development of bedaquiline and delamanid has potentially changed the treatment landscape for MDR-TB. This review provides an update on the progress of these novel antitubercular agents. Read More

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December 2018
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Utility of fine needle aspiration cytology to diagnose intraoral tuberculosis and other oral granulomatous lesions.

Diagn Cytopathol 2018 Dec 8. Epub 2018 Dec 8.

Department of ENT, Maulana Azad Medical College, Delhi, India.

Objective: Tuberculosis (TB) is a common granulomatous disease leading to high morbidity and mortality worldwide. Though rare, oral tuberculosis (OTB) may manifest during the primary and/or secondary stages of the illness. We studied the manifestations and incidence of oral granulomatous lesions (OGL) and OTB diagnosed on fine needle aspiration cytology (FNAC). Read More

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December 2018

Biosynthesis of an anti-tuberculosis sesterterpenoid asperterpenoid A.

Org Biomol Chem 2018 Dec 14. Epub 2018 Dec 14.

Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, People's Republic of China.

A putative three-gene cluster for asperterpenoid A was identified. Step-wise reconstitution of this gene cluster in Aspergillus oryzae reveals that astC encodes a sesterterpene cyclase to synthesize preasperterpenoid A, which is dually oxidized by a P450 enzyme AstB to give asperterpenoid A along with a minor product asperterpenoid B, and asperterpenoid A is further oxidized by another P450 eznyme AstA to afford a new sesterterpenoid asperterpenoid C. Unexpectedly, asperterpenoids A and B, but not the final product asperterpenoid C, exhibit potent inhibitory activity against Mycobacterium tuberculosis protein tyrosine phosphatase B with IC50 values of 3-6 μM. Read More

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December 2018
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A hotspots analysis-relation discovery representation model for revealing diabetes mellitus and obesity.

BMC Syst Biol 2018 Dec 14;12(Suppl 7):116. Epub 2018 Dec 14.

Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, College of Computer Science and Technology, Jilin University, Changchun, 130012, China.

Background: Nowadays, because of the huge economic burden on society causing by obesity and diabetes, they turn into the most serious public health challenges in the world. To reveal the close and complex relationships between diabetes, obesity and other diseases, search the effective treatment for them, a novel model named as representative latent Dirichlet allocation (RLDA) topic model is presented.

Results: RLDA was applied to a corpus of more than 337,000 literatures of diabetes and obesity which were published from 2007 to 2016. Read More

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December 2018

Characterization of Mycobacterium tuberculosis strains in Beijing, China: drug susceptibility phenotypes and Beijing genotype family transmission.

BMC Infect Dis 2018 Dec 14;18(1):658. Epub 2018 Dec 14.

Department of Bacteriology and Immunology, Beijing Key Laboratory on Drug-Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute/Beijing Chest Hospital, Capital Medical University, Beijing, 101149, China.

Background: The most prevalent strains of Mycobacterium tuberculosis (M.tb) in Beijing belong to the Beijing genotype family. The influence of Beijing genotype prevalence on the development of drug resistance, and the association of infection with Beijing genotype M. Read More

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December 2018

Improving detection of tuberculosis among household contacts of index tuberculosis patients by an integrated approach in Myanmar: a cross-sectional study.

BMC Infect Dis 2018 Dec 14;18(1):660. Epub 2018 Dec 14.

Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.

Background: Contact tracing for tuberculosis (TB) is a recommended measure to improve the case detection rate; however, actual implementation in Myanmar is limited and low detection rates have been reported. Household contacts of a known index TB case are at high risk of infection, thus a more strategic action for contact tracing is required to achieve the goal of the World Health Organization End TB Strategy. This study aimed to assess TB case detection rates among household contacts by an integrated approach and identify risk factors for TB. Read More

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December 2018

The transcription of pafA, encoding the prokaryotic ubiquitin-like protein ligase, is regulated by PafBC.

Future Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.

Aim: Mycobacterium tuberculosis possesses an intracellular tagging and degradation system, which has emerged as a target for development of anti-tuberculosis agents. In this system, PafA is the ligase that marks proteins for degradation by their covalent modification with a protein modifier. Here, we studied pafA transcriptional regulation, which remained elusive despite its importance for M. Read More

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December 2018

Dataset of single bacteria cells with different antibiotic susceptibility obtained by Raman spectroscopy.

Data Brief 2018 Dec 23;21:2430-2434. Epub 2018 Nov 23.

Immanuel Kant Baltic Federal University, A. Nevskogo St. 14, 236016 Kaliningrad, Russia.

This data article contains Raman experimental data, obtained with Horiba Jobin-Yvon LabRam HR800 spectrometer (Japan), which can be used for rapid identification of () bacteria (Beijing clade) . Data present analyzed Raman spectra of bacterial cells with various drug resistances obtained from pulmonary and extra pulmonary samples. Data can provide information about characteristic maxima of different structures in biological cell. Read More

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December 2018

Targeted Loop-Mediated Isothermal Amplification (LAMP) Assay for Consensus Detection of Mycobacteria Associated With Pulmonary Infections.

Front Med (Lausanne) 2018 28;5:332. Epub 2018 Nov 28.

Aix-Marseille Université, IRD 198, MEPHI, IHU Méditerranée Infection, Marseille, France.

Loop-mediated isothermal amplification (LAMP) is a nucleic acid method which has been used to identify mycobacteria including in clinical microbiology laboratory and point of care settings. Previously published LAMP protocols for detection of mycobacterial species used conventional specific primer and targeted the 16S rRNA, , and insertion sequence genes. We developed and evaluated a LAMP assay targeting a mycobacterial gene conserved sequence and incorporating degenerate primers. Read More

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November 2018

Treatment outcomes and factors affecting treatment outcomes of new patients with tuberculosis in Busan, South Korea: a retrospective study of a citywide registry, 2014-2015.

BMC Infect Dis 2018 Dec 13;18(1):655. Epub 2018 Dec 13.

Busan Center for Infectious Disease Control and Prevention, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, South Korea.

Background: This study investigated the treatment outcomes, and factors affecting the outcomes, of new tuberculosis (TB) patients in Busan, South Korea.

Methods: We retrospectively analysed the citywide TB registry data (collected for the Korean National TB Surveillance System) of new TB patients registered in Busan from January 2014 to December 2015.

Results: A total of 4732 patients were included in this study (mean age, 52. Read More

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December 2018
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Eravacycline activity against clinical S. aureus isolates from China: in vitro activity, MLST profiles and heteroresistance.

BMC Microbiol 2018 Dec 13;18(1):211. Epub 2018 Dec 13.

Department of Infectious Diseases and Quality Control Center of Hospital Infection Management of Shenzhen, Shenzhen Nanshan People's Hospital, Guang Dong Medical University, No 89, Taoyuan Road, Nanshan District, Shenzhen, 518052, China.

Background: Mortality rates for patients with Staphylococcus aureus (S. aureus) infections have improved only modestly in recent decades and S. aureus infections remain a major clinical challenge This study investigated the in vitro antimicrobial activity of erevacycline (erava) against clinical S. Read More

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December 2018

Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis.

Biosensors (Basel) 2018 Dec 12;8(4). Epub 2018 Dec 12.

Global Alliance for Rapid Diagnostics. Michigan State University, East Lansing, MI 48824, USA.

A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). Read More

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December 2018

Optimization and Validation of a Chromatographic Method for the Quantification of Isoniazid in Urine of Tuberculosis Patients According to the European Medicines Agency Guideline.

Antibiotics (Basel) 2018 Dec 11;7(4). Epub 2018 Dec 11.

Química Bioanalítica, Departament de Química Física i Analítica, Escola Superior de Tecnologia i Ciències Experimentals, Universitat Jaume I, 12071 Castelló, Spain.

Isoniazid is a drug that is widely used against tuberculosis. However, it shows high interpatient variability in metabolism kinetics and clinical effect, which complicates the prescription of the medication and jeopardizes the success of the therapy. Therefore, in a specific patient, the pharmacokinetics of the drug must be elucidated to decide the proper dosage and intake frequency to make the drug suitable for therapeutic drug monitoring. Read More

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December 2018

Tuberculosis Transmission in Households and Classrooms of Adolescent Cases Compared to the Community in China.

Int J Environ Res Public Health 2018 Dec 10;15(12). Epub 2018 Dec 10.

Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hatyai 90110, Songkhla, Thailand.

The aim of this paper is to evaluate the link between the history of exposure to tuberculosis (TB) in the household and diagnosed TB cases at school, and to compare the detection rate of active TB among household contacts and classroom contacts of adolescent TB cases with the rates among contacts of healthy controls. From November 2016 to December 2017, a prospective matched case-control study was conducted using passively identified index adolescent student cases from the TB surveillance system and healthy controls (matched by county, school type, sex, age and ethnicity). Contacts in households and classrooms of index cases and of controls were investigated. Read More

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December 2018
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Prevalence and incidence of Zika virus infection among household contacts of patients with Zika virus disease, Puerto Rico, 2016-2017.

J Infect Dis 2018 Dec 13. Epub 2018 Dec 13.

National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, San Juan, PR.

Background: Little is known about the prevalence or incidence of Zika virus (ZIKV) infection in settings affected by the 2015-16 Zika pandemic, and associated risk-factors. We assessed these factors among household contacts of patients with ZIKV disease enrolled in a cohort study in Puerto Rico during 2016-2017.

Methods: Household contacts of index case-patients completed a questionnaire and gave specimens for RT-PCR and IgM ELISA testing to detect ZIKV infection. Read More

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December 2018

The dynamic region of the peptidoglycan synthase gene, Rv0050, induces the growth rate and morphologic heterogeneity in Mycobacteria.

Infect Genet Evol 2018 Dec 10. Epub 2018 Dec 10.

Shanghai Key Laboratory of Tuberculosis, Clinic and Research Center of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:

Mycobacterium tuberculosis (MTB) infections rely on continued growth and division. Despite the substantial global burden of tuberculosis, the underlying mechanism governing growth is incompletely understood. Bifunctional penicillin-binding protein (PBP1), encoded by Rv0050 (ponA1) of MTB, is a key peptidoglycan synthase and plays a central role in mycobacterial growth and division by its interaction with Rpf-interacting protein A (RipA, peptidoglycan endopeptidase). Read More

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December 2018

Update on the virulence factors of the obligate pathogen Mycobacterium tuberculosis and related tuberculosis-causing mycobacteria.

Infect Genet Evol 2018 Dec 10. Epub 2018 Dec 10.

Institut Pasteur, Unit for Integrated Mycobacterial Pathogenomics, CNRS UMR3525, Paris 75015, France. Electronic address:

Over the long course of evolution from a probable environmental reservoir, the pathogen that we know today as Mycobacterium tuberculosis has become fully capable of adapting to the life inside host cells by evading and modifying their responses to infection. Factors contributing to the success of this pathogen are numerous and thanks to a large body of work accumulated over the past decades, we are closer to understanding the remarkable complexity of tuberculosis pathogenesis. The unique type VII secretion systems and various complex lipids of the cell envelope have emerged as some of the most important and most studied factors in this regard. Read More

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December 2018

Development of high throughput screening methods for inhibitors of ClpC1P1P2 from Mycobacteria tuberculosis.

Anal Biochem 2018 Dec 10;567:30-37. Epub 2018 Dec 10.

Department Cell Biology, Harvard Medical School, USA.

Tuberculosis affects about 100 million people worldwide and causes nearly 2 million deaths annually. It has been estimated that one third of all humans is infected with latent Mycobacterium tuberculosis (Mtb). Moreover, Mtb has become increasingly resistant to available antibiotics. Read More

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December 2018

Utility of whole genome sequencing for multidrug resistant Mycobacterium tuberculosis isolates in a reference TB laboratory in New Zealand.

N Z Med J 2018 Dec 14;131(1487):15-22. Epub 2018 Dec 14.

Medical Laboratory Scientist, Microbiology, LabPLUS, Auckland City Hospital, Auckland.

New Zealand has a low burden of multi-drug resistant TB (MDR-TB), but with increased mobility within the population, rapid detection and treatment of MDR-TB is a priority from the public health point of view. Mycobacterium Reference Laboratory in LabPLUS, Auckland City Hospital receives referred Mycobacterium tuberculosis complex (MTBC) isolates from all over New Zealand for second-line drug susceptibility testing (DST) and 24-loci MIRU VNTR genotyping. Between 2002 and 2013, 38 multidrug resistant Mycobacterium tuberculosis (MDR-TB) isolates were recorded by culture-based DST. Read More

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December 2018
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Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

Turk J Med Sci 2018 12 12;48(6):1351-1357. Epub 2018 Dec 12.

Department of Medical Microbiology, School of Medicine, Acıbadem University, İstanbul, Turkey

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4. Read More

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December 2018

Design and protocol for a pragmatic randomised study to optimise screening, prevention and care for tuberculosis and HIV in Malawi (PROSPECT Study).

Wellcome Open Res 2018 18;3:61. Epub 2018 May 18.

London School of Hygiene & Tropical Medicine, London, UK.

Adults seeking diagnosis and treatment for tuberculosis (TB) and HIV in low-resource settings face considerable barriers and have high pre-treatment mortality. Efforts to improve access to prompt TB treatment have been hampered by limitations in TB diagnostics, with considerable uncertainty about how available and new tests can best be implemented. The PROSPECT Study is an open, three-arm pragmatic randomised study that will investigate the effectiveness and cost-effectiveness of optimised HIV and TB diagnosis and linkage to care interventions in reducing time to TB diagnosis and prevalence of undiagnosed TB and HIV in primary care in Blantyre, Malawi. Read More

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TB Control in Humans and Animals in South Africa: A Perspective on Problems and Successes.

Front Vet Sci 2018 27;5:298. Epub 2018 Nov 27.

Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.

() remains one of the most globally serious infectious agents for human morbidity and mortality, but with significant differences in prevalence across the globe. In many countries, the incidence is now low and declining, but control and eradication remain a distant view. Similarly, the prevalence of bovine TB caused by , varies significantly across regions, although unlike for , data are sparse. Read More

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November 2018
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Electron transfer ferredoxins with unusual cluster binding motifs support secondary metabolism in many bacteria.

Chem Sci 2018 Nov 23;9(41):7948-7957. Epub 2018 Aug 23.

Department of Chemistry , University of Adelaide , SA 5005 , Australia . Email:

The proteins responsible for controlling electron transfer in bacterial secondary metabolism are not always known or characterised. Here we demonstrate that many bacteria contain a set of unfamiliar ferredoxin encoding genes which are associated with those of cytochrome P450 (CYP) monooxygenases and as such are involved in anabolic and catabolic metabolism. The model organism M contains eleven of these genes which encode [3Fe-4S] or [4Fe-4S] single cluster containing ferredoxins but which have unusual iron-sulfur cluster binding motif sequences, XXXX(X) P, where '' indicates a variable amino acid residue. Read More

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November 2018

Tuberculous peritonitis diagnosed using laparoscopy with assistance of a central venous catheter.

Exp Ther Med 2018 Dec 12;16(6):5265-5271. Epub 2018 Oct 12.

Department of Gastroenterology, Shenzhen People's Hospital, The Second Affiliated Hospital of Jinan University, Shenzhen, Guangdong 518020, P.R. China.

Laparoscopy with peritoneal biopsy is a tool for rapid and accurate diagnosis of tuberculous peritonitis (TBP). However, laparoscopic procedures are not risk-free; complications include injuries to the gastrointestinal tract and major blood vessels. The purpose of the present study was to introduce a novel method for safe and straightforward laparoscopic diagnosis of TBP. Read More

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December 2018
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Diagnostic value of medical thoracoscopy for undiagnosed pleural effusions.

Exp Ther Med 2018 Dec 17;16(6):4590-4594. Epub 2018 Sep 17.

Department of Respiratory Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.

To investigate the diagnostic value of medical thoracoscopy for the diagnosis of undiagnosed pleural effusions, a retrospective study was performed on the clinical data of 86 patients with undiagnosed pleural effusions who had medical thoracoscopy at Shaanxi Provincial People's Hospital (Xi'an, China) between May 2012 and November 2013. Of the 86 patients, 79 cases of pleural effusions were confirmed by medical thoracoscopic biopsy with a diagnosis rate of 91.9%. Read More

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December 2018

Comparative study of primary pulmonary cryptococcosis with multiple nodules or masses by CT and pathology.

Exp Ther Med 2018 Dec 17;16(6):4437-4444. Epub 2018 Sep 17.

Department of Respiratory Disease, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China.

The manifestations of pulmonary cryptococcosis with multiple nodules or masses on computed tomography (CT) are diverse and difficult to differentiate from those of lung cancer and pulmonary tuberculosis. The present study compared the multislice spiral CT signs with pathological results and used the pathological results to explain the CT signs with the aim of improving the accuracy of the diagnosis of this disease. A retrospective analysis of 20 patients with primary pulmonary cryptococcosis with multiple nodules or masses was performed. Read More

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December 2018