340,052 results match your criteria Translocation Carcinoma


Dirhodium (II) complex interferes with iron-transport system to exert antibacterial action against Streptococcus pneumoniae.

J Proteomics 2018 Dec 3. Epub 2018 Dec 3.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Drug resistance in bacteria is becoming a significant threat to global public health, and the development of novel and efficient antibacterial compounds is urgently needed. Recently, rhodium complexes have attracted attention as antimicrobial agents, yet their antibacterial mechanism remains unknown. In this study, we observed that the dirhodium (II) complex RhAc inhibited Streptococcus. Read More

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December 2018
1 Read

Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix.

Int Immunopharmacol 2018 Dec 3;66:347-353. Epub 2018 Dec 3.

Department of orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. Electronic address:

Ivabradine is most commonly used for the treatment of worsening cardiac failure in patients who cannot tolerate the maximum dose of β-blockers or in whom treatment with β-blockers is contraindicated. While ivabradine is regarded as a highly selective "funny current" (I) inhibitor, the molecular mechanism behind the effect of this drug remains poorly understood. In the present study, we applied ivabradine in the context of osteoarthritis by treating primary human chondrocytes with tumor necrosis factor-α (TNF-α) and measuring degradation of the articular cartilage matrix as well as the expression of various enzymes and pro-inflammatory cytokines. Read More

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December 2018
4 Reads

Extracellular FABP4 uptake by endothelial cells is dependent on cytokeratin 1 expression.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 3. Epub 2018 Dec 3.

Lipids and Atherosclerosis Research Unit, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili (IISPV), Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Universitat Rovira i Virgili, Reus, Spain. Electronic address:

Aims: The aim of this study is to determine the physical and functional interplay between fatty acid-binding protein 4 (FABP4) and its membrane receptor-like candidate protein, cytokeratin 1 (CK1), and to determine the effect of hindering CK1-mediated FABP4 cellular uptake on non-disturbed or metabolically stressed endothelial cells.

Methods: We monitored the direct interaction between FABP4 and CK1 using surface plasmon resonance, and the effects of blocking exogenous FABP4 (eFABP4) cellular uptake were determined by using specific siRNA to knock down the expression of CK1 in human umbilical vein endothelial cells (HUVECs). The expression and nuclear translocation of transcription factors involved in oxidative stress (NRF2) and inflammation (p65 subunit of NF-ĸB transcription factor) were determined by Western blotting analysis. Read More

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December 2018
1 Read

Endonuclease G modulates the alternative splicing of deoxyribonuclease 1 mRNA in human CD4 T lymphocytes and prevents the progression of apoptosis.

Biochimie 2018 Dec 3. Epub 2018 Dec 3.

Institute of Biomedical Chemistry, Pogodinskaya St 10/8, 119121, Moscow, Russia.

Apoptotic endonucleases act cooperatively to fragment DNA and ensure the irreversibility of apoptosis. However, very little is known regarding the potential regulatory links between endonucleases. Deoxyribonuclease 1 (DNase I) inactivation is caused by alternative splicing (AS) of DNase I pre-mRNA skipping exon 4, which occurs in response to EndoG overexpression in cells. Read More

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December 2018
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Molecular optimization of autotransporter-based tyrosinase surface display.

Biochim Biophys Acta Biomembr 2018 Dec 3. Epub 2018 Dec 3.

Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, SE 10691 Stockholm, Sweden. Electronic address:

Display of recombinant enzymes on the cell surface of Gram-negative bacteria is a desirable feature with applications in whole-cell biocatalysis, affinity screening and degradation of environmental pollutants. One common technique for recombinant protein display on the Escherichia coli surface is autotransport. Successful autotransport of an enzyme largely depends on the following: (1) the size, sequence and structure of the displayed protein, (2) the cultivation conditions, and (3) the choice of the autotransporter expression system. Read More

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December 2018

Extracellular polymeric substances (EPS) producing and oil degrading bacteria isolated from the northern Gulf of Mexico.

PLoS One 2018 6;13(12):e0208406. Epub 2018 Dec 6.

Department of Marine Biology, Texas A&M University at Galveston, Galveston, Texas, United States of America.

Sinking marine oil snow was found to be a major mechanism in the transport of spilled oil from the surface to the deep sea following the Deepwater Horizon (DwH) oil spill. Marine snow formation is primarily facilitated by extracellular polymeric substances (EPS), which are mainly composed of proteins and carbohydrates secreted by microorganisms. While numerous bacteria have been identified to degrade oil, there is a paucity of knowledge on bacteria that produce EPS in response to oil and Corexit exposure in the northern Gulf of Mexico (nGoM). Read More

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December 2018

E4orf1: A protein for enhancing glucose uptake despite impaired proximal insulin signaling.

PLoS One 2018 6;13(12):e0208427. Epub 2018 Dec 6.

Department of Nutritional Sciences, Texas Tech University, Lubbock, Texas, United States of America.

Background: Type 2 diabetes is often linked with impaired proximal insulin signaling. Hence, a therapeutic agent that enhances cellular glucose uptake without requiring proximal insulin signaling would be desirable for improving glycemic control. The E4orf1 peptide (E4) derived from human adenovirus 36 (Ad36) promotes cellular glucose uptake in vitro and in vivo, independent of insulin. Read More

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December 2018
2 Reads

Reinvestigating the status of malaria parasite (Plasmodium sp.) in Indian non-human primates.

PLoS Negl Trop Dis 2018 Dec 6;12(12):e0006801. Epub 2018 Dec 6.

TE-11, Centre for Ecological Sciences, Indian Institute of Sciences, Bangalore, India.

Many human parasites and pathogens have closely related counterparts among non-human primates. For example, non-human primates harbour several species of malaria causing parasites of the genus Plasmodium. Studies suggest that for a better understanding of the origin and evolution of human malaria parasites it is important to know the diversity and evolutionary relationships of these parasites in non-human primates. Read More

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December 2018

Two Double-hit Lymphomas Cases: A Molecular Cytogenetic Approach.

J Assoc Genet Technol 2018 ;44(4):141-145

Allina Health, Minneapolis, MN.

Objectives: Double-hit lymphomas represent 5% of cases of diffuse large B-cell lymphomas (DLBCL). They are currently recognized as highgrade B-cell lymphomas (HGBCL) with rearrangements of MYC and BCL2 and/or BCL6 by the 2016 WHO classification. One of these rearrangements is the translocation of the BCL2 gene (18q21. Read More

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January 2018
2 Reads

ATF2 promotes urothelial cancer outgrowth via cooperation with androgen receptor signaling.

Endocr Connect 2018 Dec;7(12):1397-1408

Department of Pathology & Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA.

We investigated the functional role of ATF2, a transcription factor normally activated via its phosphorylation in response to phospho-ERK/MAPK signals, in the outgrowth of urothelial cancer. In both neoplastic and non-neoplastic urothelial cells, the expression levels of androgen receptor (AR) correlated with those of phospho-ATF2. Dihydrotestosterone treatment in AR-positive bladder cancer cells also induced the expression of phospho-ATF2 and phospho-ERK as well as nuclear translocation and transcriptional activity of ATF2. Read More

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December 2018

Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response.

Hum Reprod 2018 Dec 6. Epub 2018 Dec 6.

Institute of Cell Biology and Genes and Development Group, CDBS Hugh Robson Building, University of Edinburgh, Edinburgh, UK.

Study Question: Does ovarian follicle activation by phosphatase homologue of chromosome-10 (PTEN) inhibition affect DNA damage and repair in bovine oocytes and granulosa cells?

Summary Answer: PTEN inhibition promotes bovine non-growing follicle activation but results in increased DNA damage and impaired DNA repair capacity in ovarian follicles in vitro.

What Is Known Already: Inhibition of PTEN is known to activate primordial follicles but may compromise further developmental potential. In breast cancer cells, PTEN inhibition represses nuclear translocation of breast cancer susceptibility 1 (BRCA1) and Rad51; this impairs DNA repair resulting in an accumulation of damaged DNA, which contributes to cell senescence. Read More

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December 2018

Possible cold-adaptation for the fungal kinesin in compensation for thermal stability acquired by single amino acid substitution.

J Biochem 2018 Dec 5. Epub 2018 Dec 5.

Department of Biosciences, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo, Japan.

The amino acid sequence of the motor domain of AnKinA, kinesin-1 from Aspergillus nidulans, growing optimally at 37°C, was compared with that of SbKin1, kinesin-1 from the snow mold Sclerotinia borealis. For cold-adaptation, some enzymes are thought to exhibit augmented protein structure flexibility, acquired most effectively by substituting a glycine residue for another amino acid residue. By the comparison described above, two glycine residues proximal to tightly-bound ADP were identified in the SbKin1 motor domain, of which the corresponding residues of AnKinA were non-glycine ones (P60 and S323). Read More

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December 2018
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Hereditary Spastic Paraplegia: gain-of-function mechanisms revealed by new transgenic mouse.

Hum Mol Genet 2018 Dec 6. Epub 2018 Dec 6.

Department of Neurobiology and Anatomy.

Mutations of the SPAST gene, which encodes the microtubule-severing protein spastin, are the most common cause of Hereditary Spastic Paraplegia. Haploinsufficiency is the prevalent opinion as to the mechanism of the disease, but gain-of-function toxicity of the mutant proteins is another possibility. Here, we report a new transgenic mouse (termed SPASTC448Y mouse) that is not haploinsufficient but expresses human spastin bearing the HSP pathogenic C448Y mutation. Read More

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December 2018

DNA translocation mechanism of an XPD family helicase.

Elife 2018 Dec 6;7. Epub 2018 Dec 6.

Section of Structural Biology, Department of Medicine, Imperial College London, London, United Kingdom.

The XPD family of helicases, that includes human disease-related FANCJ, DDX11 and RTEL1, are Superfamily 2 helicases that contain an iron-sulphur cluster domain, translocate on ssDNA in a 5'-3' direction and play important roles in genome stability. Consequently, mutations in several of these family members in eukaryotes cause human diseases. Family members in bacteria, such as the DinG helicase from , are also involved in DNA repair. Read More

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December 2018
5 Reads

Cloning, function, and localization of human, canine and Drosophila Zip10 (Slc39a10), a Zn transporter.

Am J Physiol Renal Physiol 2018 Dec 6. Epub 2018 Dec 6.

Physiology & Biomedical Engineering, Mayo Clinic College of Medicine, United States.

Zinc (Zn) is the second most abundant trace element, but is considered a micronutrient as it is a cofactor for many enzymes and transcription factors. While Zn deficiency can cause cognitive immune or metabolic dysfunction and infertility, excess Zn is nephrotoxic. As for other ions and solutes, Zn is moved into and out of cells by specific membrane transporters: ZnT, Zip, and NRAMP/DMT proteins. Read More

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December 2018
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Characterization of the Blister Fluid Proteome for Pediatric Burn Classification.

J Proteome Res 2018 Dec 6. Epub 2018 Dec 6.

Blister fluid (BF) is a novel and viable research matrix for burn injury study, which can reflect both systemic and local micro-environmental responses. The protein abundance in BF from different burn severities were initially observed using a 2D SDS-PAGE approach. Subsequently, a quantitative data independent acquisition (DIA) method - SWATHTM was employed to characterize the proteome of pediatric burn blister fluid. Read More

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December 2018
2 Reads

Urine proteomic profiling in patients with nephrolithiasis and cystinuria.

Int Urol Nephrol 2018 Dec 5. Epub 2018 Dec 5.

Nephrology Division, New York University Langone Medical Center, New York, NY, USA.

Purpose: The purpose of the study was to assess the differences in the concentration and function of urinary proteins between patients with cystine stones (CYS) and healthy controls (HC). We postulated that CYS and HC groups would demonstrate different proteomic profiles.

Methods: A pilot study was performed comparing urinary proteomes of 10 patients with CYS and 10 age- and gender-matched HC, using liquid chromatography-mass spectrometry. Read More

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December 2018
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Significance of Cholesterol-Binding Motifs in ABCA1, ABCG1, and SR-B1 Structure.

J Membr Biol 2018 Dec 6. Epub 2018 Dec 6.

National Research Centre for Preventive Medicine, 10, Petroverigsky Street, Moscow, Russia, 101990.

ABCA1, ABCG1 transporters, and SR-B1 receptor are the major proteins involved in cholesterol efflux from cells. We superposed in silico the location of putative cholesterol (Chol)-binding motifs CRAC/CARC and CCM in human ABCA1, ABCG1, and SR-B1 with (1) transmembrane protein topology, (2) a profile of structural order of protein, and (3) with an influence of single amino acid substitutions on protein structure and function. ABCA1, ABCG1, and SR-B1 molecules contain 50, 19, and 13 Chol-binding motifs, respectively, that are localized either in membrane helices, or at membrane-water interface, or in water-exposed protein regions. Read More

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December 2018
2 Reads

Mitochondrial Electron Transport Chain Complex Dysfunction in MeCP2 Knock-Down Astrocytes: Protective Effects of Quercetin Hydrate.

J Mol Neurosci 2018 Dec 6. Epub 2018 Dec 6.

Division of Neurobiology, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India.

Astrocytes play the central role in CNS metabolism to support neuronal functions. Mehyl-CpG-binding protein 2 (MeCP2) is the global transcription factor with differential expression in neuronal and non-neuronal cells. MeCP2 mutation and downstream detrimental effects have been reported in astrocytes also in MeCP2-associated neurodevelopmental disorder-Rett syndrome. Read More

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December 2018
5 Reads

Correlation analysis of the transcriptome and metabolome reveals the regulatory network for lipid synthesis in developing Brassica napus embryos.

Plant Mol Biol 2018 Dec 5. Epub 2018 Dec 5.

Animal Sciences National Teaching Demonstration Center, Nanjing Agricultural University, Nanjing, 210095, China.

Key Message: In this manuscript, we explored the key molecular networks for oil biosynthesis with the transcriptome and metabolome of B. napus embryo at different developmental stages. Brassica napus (B. Read More

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December 2018
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Targeted disruption of PI3K/Akt/mTOR signaling pathway, via PI3K inhibitors, promotes growth inhibitory effects in oral cancer cells.

Cancer Chemother Pharmacol 2018 Dec 5. Epub 2018 Dec 5.

Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.

Purpose: The phosphoinositide-3-kinase (PI3K) pathway is the frequently altered in human cancer. This has led to the development and study of novel PI3K inhibitors for targeted therapy and also to overcome resistance to radiotherapy.

Method: The anti-tumour effects of PI3K inhibitors (PI-828, PI-103 and PX-866) in terms of cell proliferation, colony formation, induction of apoptosis, cell cycle arrest, invasion, autophagy, and pNF-κB/p65 translocation in SCC-4, SCC-9 and SCC-25 cells were studied by performing MTT, clonogenic, DAPI staining, propidium iodide staining, annexin-V binding, matrigel invasion, acridine orange staining and immuno-fluorescence assay. Read More

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December 2018
2 Reads

Cyclic Stretch Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via YAP Activation.

Biomed Res Int 2018 5;2018:2174824. Epub 2018 Nov 5.

State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, China.

Periodontal remodeling and alveolar bone resorption and formation play essential roles during orthodontic tooth movement (OTM). In the process, human periodontal ligament cells (HPDLCs) sense and respond to orthodontic forces, contributing to the alveolar bone formation. However, the underlying mechanism in this process is not fully elucidated. Read More

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November 2018

Physicochemical properties of air discharge-generated manganese oxide nanoparticles: Comparison to welding fumes.

Environ Sci Nano 2018 15;2018(5):696-707. Epub 2018 Jan 15.

Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA.

Exposures to high doses of manganese (Mn) via inhalation, dermal contact or direct consumption can cause adverse health effects. Welding fumes are a major source of manganese containing nanoparticles in occupational settings. Understanding the physicochemical properties of manganese-containing nanoparticles can be a first step in understanding their toxic potential following exposure. Read More

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January 2018
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A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates.

BMC Biophys 2018 29;11. Epub 2018 Nov 29.

Department of Biochemistry and Molecular Biology, Campusvej 55, Odense M, 5230 Denmark.

Background: Intracellular phase separation and aggregation of proteins with extended poly-glutamine (polyQ) stretches are hallmarks of various age-associated neurodegenerative diseases. Progress in our understanding of such processes heavily relies on quantitative fluorescence imaging of suitably tagged proteins. Fluorescence loss in photobleaching (FLIP) is particularly well-suited to study the dynamics of protein aggregation in cellular models of Chorea Huntington and other polyQ diseases, as FLIP gives access to the full spatio-temporal profile of intensity changes in the cell geometry. Read More

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November 2018
2 Reads

Draft genomes of strains isolated from dried spices bring unique insights into the diversity of plant-associated strains.

Stand Genomic Sci 2018 29;13:35. Epub 2018 Nov 29.

1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708 USA.

is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of , which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the genomes was 4393 kb, with an average of 4055 protein coding genes, and an average genome G + C content of 56. Read More

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November 2018
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Paper-based immunosensor utilizing dielectrophoretic trapping of microprobes for quantitative and label free detection using electrochemical impedance spectroscopy.

Biomicrofluidics 2018 Nov 13;12(6):064102. Epub 2018 Nov 13.

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan.

In this study, we have developed a novel paper based immunoassay for the quantitative detection of immunoreactions using electrochemical impedance spectroscopy. Paper provides an attractive platform for fabrication of simple, low cost, and portable diagnostic devices as it allows passive liquid transport, is biocompatible, and has tunable properties such as hydrophilicity, flexibility, permeability, and reactivity. We have used screen-printing to fabricate interdigitated electrodes (finger width and gap of 200 m) on the paper substrate, while UV-lithography enables patterning of the paper into hydrophobic/hydrophilic regions. Read More

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November 2018

Tenascin-C Modulates Cell Cycle Progression to Enhance Tumour Cell Proliferation through AKT/FOXO1 Signalling in Pancreatic Cancer.

J Cancer 2018 31;9(23):4449-4462. Epub 2018 Oct 31.

School of medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.

Pancreatic ductal adenocarcinoma (PDAC) is a disease with an extremely poor prognosis that is characterized by a rich extracellular matrix (ECM). Tenascin-C (TNC) is a component of the ECM and plays a role in tumour progression. In this study, we reported that TNC is overexpressed in PDAC tissues and is correlated with tumour stage and cyclin D1 expression. Read More

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October 2018

Comprehensive Analysis of Somatic Mutation in Clear Cell Renal Cell Carcinoma to Explore Potential Mechanisms .

J Cancer 2018 18;9(22):4108-4116. Epub 2018 Oct 18.

Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Aim of this study was to comprehensively analyze BRCA1-associated protein-1 () somatic mutation in clear cell renal cell carcinoma (ccRCC) and explore potential therapeutic pathways and molecules. In this study, we analyzed 445 ccRCC cases from The Cancer Genome Atlas (TCGA). Comprehensive analysis including survival, transcriptome and methylation between mutated and wild-type cases was performed using bioinformatics tools . Read More

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October 2018

Simultaneous consumption of cellobiose and xylose by to circumvent glucose repression and identification of its cellobiose-assimilating operons.

Biotechnol Biofuels 2018 1;11:320. Epub 2018 Dec 1.

1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.

Background: The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primarily glucose and xylose, is critical for the viability of lignocellulosic biorefineries. However, the phenomenon of glucose repression prevents co-utilization of both glucose and xylose in cellulosic hydrolysates. Read More

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December 2018

Plant Frataxin in Metal Metabolism.

Front Plant Sci 2018 21;9:1706. Epub 2018 Nov 21.

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario, Argentina.

Frataxin is a highly conserved protein from prokaryotes to eukaryotes. Several functions related to iron metabolism have been postulated for this protein, including Fe-S cluster and heme synthesis, response to oxidative damage and oxidative phosphorylation. In plants, the presence of one or two isoforms of this protein with dual localization in mitochondria and chloroplasts has been reported. Read More

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November 2018
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Mechanisms of deformable nanovesicles based on insulin-phospholipid complex for enhancing buccal delivery of insulin.

Int J Nanomedicine 2018 9;13:7319-7331. Epub 2018 Nov 9.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China,

Background: Non-injectable delivery of peptides and proteins are not feasible due to its large molecular, high hydrophilic and gastrointestinal degradation. Therefore, proposing a new method to solve this problem is a burning issue.

Purpose: The objective of this study was to propose a novel protein delivery strategy to vanquish the poor efficacy of buccal mucosa delivery systems for protein delivery and then investigate the detailed mechanisms of the enhanced buccal delivery of protein, using insulin as a model drug. Read More

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November 2018

Mitochondrial functioning abnormalities observed in blood platelets of chronic smoke-exposed guinea pigs - a pilot study.

Int J Chron Obstruct Pulmon Dis 2018 9;13:3707-3717. Epub 2018 Nov 9.

Department of Pneumology and Allergy, Medical University of Lodz, Lodz, Poland,

Background: COPD represents a major global health issue, which is often accompanied by cardiovascular diseases. A considerable body of evidence suggests that cardiovascular risk is elevated by the activation of blood platelets, which in turn is exacerbated by inflammation. As reactive oxygen species are believed to be an important factor in platelet metabolism and functioning, the aim of our study was to perform a complex assessment of mitochondrial function in platelets in chronic smoke exposed animals with COPD-like lung lesions. Read More

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November 2018
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High Abundance of genus Prevotella in the gut of perinatally HIV-infected children is associated with IP-10 levels despite therapy.

Sci Rep 2018 Dec 5;8(1):17679. Epub 2018 Dec 5.

Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.

Perinatal HIV infection is characterized by faster HIV disease progression and higher initial rate of HIV replication compared to adults. While antiretroviral therapy (ART) has greatly reduced HIV replication to undetectable levels, there is persistent elevated inflammation associated with HIV disease progression. Alteration of gut microbiota is associated with increased inflammation in chronic adult HIV infection. Read More

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December 2018

Targeting of chondrocyte plasticity via connexin43 modulation attenuates cellular senescence and fosters a pro-regenerative environment in osteoarthritis.

Cell Death Dis 2018 Dec 5;9(12):1166. Epub 2018 Dec 5.

CellCOM research Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), Servizo Galego de Saúde (SERGAS), Universidade da Coruña (UDC), Xubias de Arriba, 84, 15006 A, Coruña, Spain.

Osteoarthritis (OA), a chronic disease characterized by articular cartilage degeneration, is a leading cause of disability and pain worldwide. In OA, chondrocytes in cartilage undergo phenotypic changes and senescence, restricting cartilage regeneration and favouring disease progression. Similar to other wound-healing disorders, chondrocytes from OA patients show a chronic increase in the gap junction channel protein connexin43 (Cx43), which regulates signal transduction through the exchange of elements or recruitment/release of signalling factors. Read More

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December 2018

HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.

Cell Death Dis 2018 Dec 5;9(12):1176. Epub 2018 Dec 5.

Jiangsu Cancer Biotherapy Institute, Xuzhou Medical University, Xuzhou, Jiangsu, P. R. China.

Hepatocellular carcinoma-related protein-1 (HCRP-1), a subunit of mammalian endosomal sorting complex required for transport-I (ESCRT-I), is frequently downregulated in various kinds of malignant tumors. The role of HCRP-1 in colorectal cancer (CRC) remains unknown. We investigate the clinical value of HCRP-1 and its impact on anoikis in CRC. Read More

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December 2018
1 Read

Correction: Yes and Lyn play a role in nuclear translocation of the epidermal growth factor receptor.

Oncogene 2018 Dec 5. Epub 2018 Dec 5.

Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.

In Figure 4C, it was identified that the Histone H3 and α-Tubulin purification control blots for YES and LYN overexpressing cells were duplicated. The original Histone H3 control blot was found and confirmed the published results, however, the α-Tubulin control blot was not found. This error was determined to not impact the scientific findings of this figure. Read More

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December 2018

Targeting the BRD4/FOXO3a/CDK6 axis sensitizes AKT inhibition in luminal breast cancer.

Nat Commun 2018 Dec 5;9(1):5200. Epub 2018 Dec 5.

Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY, 40506, USA.

BRD4 assembles transcriptional machinery at gene super-enhancer regions and governs the expression of genes that are critical for cancer progression. However, it remains unclear whether BRD4-mediated gene transcription is required for tumor cells to develop drug resistance. Our data show that prolonged treatment of luminal breast cancer cells with AKT inhibitors induces FOXO3a dephosphorylation, nuclear translocation, and disrupts its association with SirT6, eventually leading to FOXO3a acetylation as well as BRD4 recognition. Read More

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December 2018
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Structure of a functional obligate complex IIIIV respiratory supercomplex from Mycobacterium smegmatis.

Nat Struct Mol Biol 2018 Dec 5. Epub 2018 Dec 5.

Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden.

In the mycobacterial electron-transport chain, respiratory complex III passes electrons from menaquinol to complex IV, which in turn reduces oxygen, the terminal acceptor. Electron transfer is coupled to transmembrane proton translocation, thus establishing the electrochemical proton gradient that drives ATP synthesis. We isolated, biochemically characterized, and determined the structure of the obligate IIIIV supercomplex from Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. Read More

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December 2018
2 Reads

Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation.

J Virol 2018 Dec 5. Epub 2018 Dec 5.

Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China

The type I interferon (IFN) response is the first line of host innate immune defense against viral infection; however, viruses have developed multiple strategies to antagonize host IFN responses for efficient infection and replication. Here, we report that Marek's disease virus (MDV), an oncogenic herpesvirus, encodes VP23 protein as a novel immune modulator to block IFN-β activation induced by cyclic GMP-AMP synthase (cGAS) and stimulator of interferon genes (STING) in chicken fibroblasts and macrophages. VP23 overexpression markedly reduces viral DNA-triggered IFN-β production and promotes viral replication, while knockdown of VP23 during MDV infection enhances the IFN-β response and suppresses viral replication. Read More

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December 2018

Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish ().

J Exp Biol 2018 Dec 5;221(Pt 23). Epub 2018 Dec 5.

Department of Biology, McMaster University, Hamilton, Ontario, Canada, L8S 4K1.

Many fish experience daily cycles of hypoxia in the wild, but the physiological strategies for coping with intermittent hypoxia are poorly understood. We examined how killifish adjust O supply and demand during acute hypoxia, and how these responses are altered after prolonged acclimation to constant or intermittent patterns of hypoxia exposure. We acclimated killifish to normoxia (∼20 kPa O), constant hypoxia (2 kPa) or intermittent cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28 days, and then compared whole-animal O consumption rates ( ) and tissue metabolites during exposure to 12 h of hypoxia followed by reoxygenation in normoxia. Read More

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December 2018

Chromosomal translocations and semen quality: A study on 144 male translocation carriers.

Reprod Biomed Online 2018 Nov 13. Epub 2018 Nov 13.

AP-HP, Reproductive Biology Unit, Paris-Sud University, Paris-Saclay University, Antoine Béclère Hospital, Clamart 92140, France.

Research Question: Chromosomal translocations are known genetic causes of male infertility. Are certain translocations or chromosomal regions more directly associated with sperm defects? Is there a threshold of sperm impairment that can be relevant for detection of translocations?

Design: This is a monocentric retrospective observational study covering a 10-year period. Eighty-one patients carrying a reciprocal translocation (RCT) and 63 carrying a Robertsonian translocation (ROBT) were compared with 105 fertile patients. Read More

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November 2018

The composition of a bioprocessed shiitake (Lentinus edodes) mushroom mycelia and rice bran formulation and its antimicrobial effects against Salmonella enterica subsp. enterica serovar Typhimurium strain SL1344 in macrophage cells and in mice.

BMC Complement Altern Med 2018 Dec 5;18(1):322. Epub 2018 Dec 5.

Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, 94710, USA.

Background: Human infection by pathogenic Salmonella bacteria can be acquired by consuming of undercooked meat products and eggs. Antimicrobial resistance against antibiotics used in medicine is also a major concern. To help overcome these harmful effects on microbial food safety and human health, we are developing novel antimicrobial food-compatible formulations, one of which is described in the present study. Read More

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December 2018
2 Reads

Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development.

BMC Plant Biol 2018 Dec 5;18(1):332. Epub 2018 Dec 5.

University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Urrbrae, South Australia, 5064, Australia.

Background: In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped and identified to encode a glycosylphosphatidylinositol (GPI)-anchored non-specific lipid transfer protein (nsLTP). Although this gene is critical for pollen exine development, details of its function remains poorly understood. Read More

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December 2018
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Chlorogenic Acid (CGA) Isomers Alleviate Interleukin 8 (IL-8) Production in Caco-2 Cells by Decreasing Phosphorylation of p38 and Increasing Cell Integrity.

Int J Mol Sci 2018 Dec 4;19(12). Epub 2018 Dec 4.

Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver, BC V6T 1Z4, Canada.

The objective of this study was to determine the effect of six chlorogenic acid (CGA) isomers known to be present in coffee and other plant foods on modulating the inflammatory response induced by pro-inflammatory cytokines in the Caco-2 human intestinal epithelial cell line. Compared to caffeoylquinic acids (CQA), dicaffeoylquinic acids (DiCQA) had significantly stronger ( < 0.05) capacities to reduce phosphorylation of one of mitogen-activated protein kinases (MAPK) cascades, namely p38. Read More

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December 2018

iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet ( L.).

Int J Mol Sci 2018 Dec 4;19(12). Epub 2018 Dec 4.

Lanzhou Institute of Husbandry and Pharmaceutical Science, CAAS, Lanzhou 730050, China.

Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet ( L.) to salt, in the present study, the proteomics of roots and shoots in three-week-old sugar beet plants exposed to 50 mM NaCl for 72 h was investigated by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology. Read More

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December 2018

Characterization of the Intrinsic Phospholipase A1 Activity of Adenylate Cyclase Toxin.

Toxins (Basel) 2018 Dec 4;10(12). Epub 2018 Dec 4.

Department of Biochemistry and Molecular Biology, Biofisika Institute (UPV/EHU, CSIC), University of Basque Country, Aptdo. 644, 48080 Bilbao, Spain.

Adenylate cyclase toxin (ACT, CyaA) is one of the important virulence factors secreted by the whooping cough bacterium , and it is essential for the colonization of the human respiratory tract by this bacterium. Cytotoxicity by ACT results from the synergy between toxin's two main activities, production of supraphysiological cAMP levels by its N-terminal adenylate cyclase domain (AC domain), and cell membrane permeabilization, induced by its C-terminal pore-forming domain (hemolysin domain), which debilitate the host defenses. In a previous study we discovered that purified ACT is endowed with intrinsic phospholipase A1 (PLA) activity and that Ser in position 606 of the ACT polypeptide is a catalytic site for such hydrolytic activity, as part of G-X-S-X-G catalytic motif. Read More

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December 2018
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Uptake and translocation of Cd and Zn in two lettuce cultivars.

An Acad Bras Cienc 2014 Jun;86(2):907-922

Departamento de Solos, Universidade Federal de Viçosa, Viçosa, MG, Brasil.

Excess of heavy metals in agricultural soils is a matter of concern since it may decrease economic yield as a result of toxicity and lower product quality as a result of metal accumulation in edible plant parts. Among plant species and among cultivars within species a natural variation in uptake, translocation and distribution of trace elements occur. The transference of Cd and Zn, from soil to two lettuce (Lactuca sativa L. Read More

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EPSPS duplication and mutation involved in glyphosate resistance in the allotetraploid weed species Poa annua L.

Pest Manag Sci 2018 Dec 2. Epub 2018 Dec 2.

Department of Plant Sciences, University of California, Davis, Davis, California 95616, United States.

Background: Poa annua is a widespread winter annual weed species in California. Recently, poor control of this species with glyphosate was reported by growers in an almond orchard in California with history of repetitive glyphosate use. The objectives of this research were to evaluate the level of glyphosate resistance in a developed S P. Read More

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December 2018

Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.

J Cell Mol Med 2018 Dec 3. Epub 2018 Dec 3.

Heart Health Center, East Hospital, Tongji University School of Medicine, Shanghai, China.

Nucleoporins (Nups) are known to be functional in nucleo-cytoplasmic transport, but the roles of nucleoporins in nonproliferating cells, such as cardiac myocytes, are still poorly understood. In this study, we report that Nup107 regulates cardiac bioelectricity by controlling the nucleo-cytoplasmic trafficking of Scn5a mRNA. Overexpression of Nup107 induced the protein expression of Scn5a rather than that of other ion channels, with no effects of their mRNA levels. Read More

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December 2018