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Am J Case Rep 2021 May 13;22:e931107. Epub 2021 May 13.

Department of Internal Medicine, Lyon Civil Hospices, Edouard Herriot Hospital, Lyon, France.

BACKGROUND Delayed hemolytic transfusion reactions (DHTR) are life-threatening complications mostly triggered by red blood cell (RBC) transfusions in patients with hemoglobinopathy. CASE REPORT We present a case of DHTR and hyperhemolysis syndrome in a 39-year-old pregnant woman with a history of ß-thalassemia intermediate in whom the hemoglobin (Hb) level fell to 27 g/L after transfusion of 2 units of crossmatch-compatible packed RBCs. No allo- or auto-antibody formation was detected. Read More

Transfusion 2021 May 13. Epub 2021 May 13.

Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.

Background: Delayed hemolytic transfusion reactions (DHTRs) are reported to be rare occurrences but may be more frequently observed in the trauma setting where patients are heavily transfused, followed over long inpatient admissions, and have frequent subsequent blood counts as they undergo multiple surgical interventions.

Study Design And Methods: We examined the rates of DHTRs on a per transfusion and per patient basis in an academic county hospital with a level 1 trauma center serving a four-state region and over a 3-year period. DHTRs were entered sequentially into a registry as they were observed, and a retrospective review of all new alloantibodies detected was performed to identify any additional DHTRs. Read More

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):400-403

Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.

Objectives: Thoracoabdominal aortic aneurysm (TAAA) prosthetic vessel replacement is one of the most complex operations in the field of cardiovascular surgery. The key to success of this operation is to prevent and avoid ischemia of important organs while repairing TAAA. This study aims to summarize and analyze the effect of prosthetic vessel replacement under left heart bypass in the treatment of TAAA. Read More

Transfus Clin Biol 2021 May 6. Epub 2021 May 6.

Department of Biochemistry, Government Medical College and Hospital, Chandigarh, India.

Objectives: During plateletpheresis, citrate induces hypocalcemia and hypomagnesemia, which are usually transient and self-limiting, but they can lead to significant donor discomfort. The aim of study was to determine the effect of citrate infusion on a multitude of biochemical parameters during plateletpheresis in healthy donors and to correlate changes with adverse donor reactions.

Methods: The study was conducted on 60 healthy plateletpheresis donors. Read More

Transfus Clin Biol 2021 May 6. Epub 2021 May 6.

Netaji Nagar, 753010 Cuttack, Odisha, India; DIRECTOR MEDICAL EDUCATION & TRAINING, Bhubaneswar, Odisha, India.

Background: COVID 19 infection caused by novel coronavirus with no specific established treatment. Convalescent Plasma Therapy has been authorized as an off-label therapeutic procedure. We assessed the outcome of convalescent plasma (CP) units versus standard treatment on the complete recovery, improvement and 28 days' mortality of COVID 19 patients. Read More

Mol Genet Genomic Med 2021 May 8:e1701. Epub 2021 May 8.

Blood transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Objectives: Serological methods may not be reliable for RBC antigen typing, especially in multi-transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping. Read More

Immunohematology 2021 Mar;37(1):20-24

Department of Immunology and Transfusion Medicine, St. Olavs Hospital, Trondheim University Hospital , Trondheim , Norway .

The red blood cell (RBC) antigen Wr is a low-prevalence antigen first described in 1953 by Holman and assigned to the Diego system in 1995. Because of its low prevalence, Wr is usually absent on commercial screening RBCs and antibody identification panels. When Wr(a+) screening RBCs are available, the corresponding antibody, anti-Wr, is often found in sera from healthy individuals, patients, and pregnant women. Read More

Immunohematology 2021 Mar;37(1):13-17

Department of Transfusion Medicine and Transplantation Biology, University Clinical Hospital Zagreb , Zagreb , Croatia .

Many patients with anti-Yt receive multiple transfusions of Yt(a+) red blood cells (RBCs) with no ill effects. However, anti-Yt has been implicated in hemolytic transfusion reactions. Antibody identification typically determines specificity of antibodies and their clinical significance to justify blood requirements for antigen-negative blood when clinically significant antibodies are involved. Read More

J Vet Intern Med 2021 May 7. Epub 2021 May 7.

Department of Clinical Sciences and Services, The Royal Veterinary College, North Mymms, Herts, United Kingdom.

Background: Leukoreduction of blood products is commonly performed in human medicine, but its effect on outcome or incidence of transfusion reactions (TRs) in dogs is unknown.

Objectives: To prospectively evaluate the incidence of acute TRs in, and the outcome of, dogs receiving either leukoreduced (LR) or nonleukoreduced (N-LR) packed red blood cells (PRBC).

Animals: Dogs (n = 194) administered PRBC between August 2017 and June 2020. Read More

Mayo Clin Proc 2021 05;96(5):1368-1369

Department of Hematology and Transfusion Medicine, Carlo Poma Hospital, Mantova, Italy.

Anticancer Res 2021 May;41(5):2569-2573

Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Background: Hepatocellular carcinoma (HCC) is considered a leading cause of death in patients with haemophilia. Recent advances in the treatment of unresectable HCC with molecular-targeted agents (MTAs) have led to better clinical outcomes. However, the tolerability of MTAs by haemophilic patients with HCC remains unclear. Read More

J Clin Apher 2021 May 5. Epub 2021 May 5.

Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Background: Criteria for selection of FFP blood type has not been clearly established and use of group AB plasma is preferred by numerous transplantation protocols.

Aims: This study assesses the safety and efficacy of alternative group A or B plasma in ABO incompatible solid organ transplantation.

Materials & Methods: Alternative use of group A or B plasma (incompatible plasma) was inevitable during the shortage of group AB plasma. Read More

Transfusion 2021 May 4. Epub 2021 May 4.

Blood Transfusion Service, Azienda Ospedaliera Universitaria, Ferrara, Italy.

Background: The average hemoglobin content of red cell concentrates (RCC) varies depending on the method of preparation. Surprisingly less data are available concerning the clinical impact of those differences.

Study Design And Methods: The effects of two types of RCC (RCC-A, RCC-B) on transfusion regime were compared in a non-blinded, prospective, randomized, two-period, and crossover clinical trial. Read More

Transfus Apher Sci 2021 Apr 30:103152. Epub 2021 Apr 30.

Health Sciences University, Ankara City Hospital, Division of Hematology and Oncology, Ankara, Turkey. Electronic address:

Allergic transfusion reactions (ATRs)are a common form of acute transfusion reaction. It was aimed to determine the clinical characteristics and frequency of ATRs in children. This study included children who were transfused with red cell concentrate (RCC), fresh-frozen plasma (FFP), platelet concentrates(PC), apheresis granulocyte, and cryoprecipitate. Read More

Trials 2021 May 4;22(1):323. Epub 2021 May 4.

McMaster Centre for Transfusion Research, McMaster University, Hamilton, Ontario, Canada.

Background: Convalescent plasma has been used for numerous viral diseases including influenza, severe acute respiratory syndrome, Middle East respiratory syndrome and Ebola virus; however, evidence to support its use is weak. SARS-CoV-2 is a novel coronavirus responsible for the 2019 global pandemic of COVID-19 community acquired pneumonia. We have undertaken a randomized controlled trial to assess the efficacy and safety of COVID-19 convalescent plasma (CCP) in patients with SARS-CoV-2 infection. Read More

J Clin Apher 2021 May 4. Epub 2021 May 4.

Department of Transfusion Medicine, Institute of Liver and Biliary Sciences, New Delhi, India.

Background: Adverse donor events (ADEs) are usually mild and short-term with no sequelae, but may cause disinclination toward future donations.

Aims: To determine the impact of delayed ADEs (D-ADEs) in addition to immediate ADEs (I-ADEs) on the intention of future donations (IFDs) and to analyze the various associated factors.

Methods: ADEs were categorized following the ISBT working group on donor vigilance. Read More

J Pak Med Assoc 2021 Feb;71(2(B)):699-703

Department of Pathology, Shaukat Khanum Memorial Cancer Hospital, Lahore, Pakistan.

Objective: To monitor the frequencies of different adverse transfusion reactions and to assess the compliance of clinical staff with the process of sending proper transfusion reaction workup within the specified time.

Methods: The retrospective audit was conducted at the blood bank of Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan, and comprised all transfusion reaction forms received from July 1, 2017, to June 30, 2018. The forms were analysed for type of blood component, time in which it was received by the blood bank, whether or not the form was completely filled, whether or not all required samples were provided, and the type of reaction. Read More

Ann Clin Lab Sci 2021 Mar;51(2):267-270

Department of Pathology, University of Maryland School of Medicine and University of Maryland Medical Center, Baltimore MD, USA.

Managing a platelet blood product inventory in a hospital-based transfusion service (TS) is challenging. Thus, to optimize platelet inventory availability and to prevent excess outdating, most tertiary care center-based TSs do not require ABO-identical platelet (PLT) transfusions. To mitigate the risk of hemolysis associated with the transfusion of high titer ABO antibody-containing PLT, our institutional policy allows the transfusion of PLT containing ABO-incompatible plasma only if PLT is re-suspended in platelet additive solution (PAS). Read More

Vet J 2021 Jun 26;272:105663. Epub 2021 Mar 26.

Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Major crossmatch testing can help identify immunologic incompatibilities between blood donors and recipients; however, there are limited studies describing the accuracy of point-of-care crossmatch tests. The first aim of this study was to determine if a gel-based, point-of-care major crossmatch method (GEL-CM), without antiglobulin-enhancement, could accurately detect compatible and incompatible donor-recipient pairings, using an antiglobulin-enhanced laboratory-based major crossmatch method (LAB-CM) as the reference standard. The second aim was to describe the incidence of, and risk factors for, major crossmatch incompatibility in cats. Read More

J Clin Lab Anal 2021 May 3:e23802. Epub 2021 May 3.

Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria.

Background: Stroke is a devastating complication of sickle cell anemia (SCA) and can be predicted through abnormally high cerebral blood flow velocity using transcranial Doppler Ultrasonography (TCD). The evidence on the role of alpha-thalassemia and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the development of stroke in children with SCA is conflicting. Thus, this study investigated the association of alpha-thalassemia and G6PD(A ) variant with abnormal TCD velocities among Nigerian children with SCA. Read More

Transfus Med Rev 2021 Apr 2. Epub 2021 Apr 2.

Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Despite advances in transfusion safety, concerns with safety of platelet transfusions remain including platelet-related sepsis and higher reaction rates observed among patients receiving apheresis platelets (APLTs). National Healthcare Safety Network (NHSN) Hemovigilance Module (HM) data were analyzed to quantify the burden and severity of adverse reactions occurring from APLTs and whole blood-derived platelets (WBD-PLTs). Facilities participating in NHSN HM during 2010-2018 were included. Read More

Transfus Apher Sci 2021 Apr 24:103147. Epub 2021 Apr 24.

Department of Hematology and Oncology, Okayama University Hospital, Okayama, Japan.

Peripheral blood progenitor cells (PBPCs) are a predominant graft source in allogeneic hematopoietic cell transplantation. Citrate-induced hypocalcemia remains the most frequent side effect of PBPC apheresis. Although the method for preventing severe adverse events is established, more efficient prophylaxis is required so that volunteer donors can donate PBPCs without pain and anxiety. Read More

Viruses 2021 04 16;13(4). Epub 2021 Apr 16.

Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.

Coronavirus Disease 2019 (COVID-19) is a global pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While detection of SARS-CoV-2 by polymerase chain reaction with reverse transcription (RT-PCR) is currently used to diagnose acute COVID-19 infection, serological assays are needed to study the humoral immune response to SARS-CoV-2. Anti-SARS-CoV-2 immunoglobulin (Ig)G/A/M antibodies against spike (S) protein and its receptor-binding domain (RBD) were characterized in recovered subjects who were RT-PCR-positive ( = 153) and RT-PCR-negative ( = 55) using an enzyme-linked immunosorbent assay (ELISA). Read More

J Clin Med 2021 Apr 14;10(8). Epub 2021 Apr 14.

Department of Regenerative Oral Surgery, Institute of Biomedical Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.

The purpose of this clinical study is to evaluate the safety and preliminary efficacy of autologous freeze-drying platelet-rich plasma (FD-PRP) on bone regeneration in maxillary sinus floor augmentation as a preliminary pilot study. Five patients that required sinus floor augmentation to facilitate the placement of dental implants participated in this clinical study. The PRP was prepared from the autologous peripheral blood and was lyophilized and stored at -20 °C for 4 weeks before surgery. Read More

Int J Hematol 2021 Apr 27. Epub 2021 Apr 27.

Department of Hematology and Oncology, Okayama University Hospital, 2-5-1 Shikata-cho, Kita-ku, Okayama, Okayama, 700-8558, Japan.

Waldenström macroglobulinemia (WM)/lymphoplasmacytic lymphoma (LPL) is a rare indolent B-cell neoplasm, and a gain-of-function mutation in the myeloid differentiation primary response 88 (MYD88), L265P, is a commonly recurring mutation in patients with WM/LPL. Histological transformation of WM/LPL to an aggressive lymphoma such as diffuse large B-cell lymphoma (DLBCL) is rare, and transformed DLBCL has a worse prognosis than de novo DLBCL, partly because transformed DLBCL is mostly classified as non-germinal center B-cell-like (non-GCB) subtype. We herein describe a 75-year-old man with DLBCL with a history of WM/LPL. Read More

Medicine (Baltimore) 2021 Apr;100(17):e25567

Neurosurgery Section, Department of Surgery.

Abstract: Surgical site infections (SSIs) are common complications after spinal surgery that result in increased morbidity, mortality, and healthcare costs. It was estimated that SSIs after spinal surgery resulted in a 4-fold increase in health care costs. The reported SSI rate following spinal surgery remains highly variable between approximately 0. Read More

Transfusion 2021 Apr 27. Epub 2021 Apr 27.

Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA.

Background: Convalescent plasma (CP) is an important initial treatment in pandemics and the New York (NY) metropolitan area is likely to remain a hotspot for collection and distribution of such units. This study reports characteristics of coronavirus disease 19 CP (CCP) donors and their donations to the New York Blood Center (NYBC).

Study Design And Methods: All CCP data from our first day of collection on March 26th through July 7th, 2020 are included in this retrospective analysis. Read More

Transfusion 2021 Apr 26. Epub 2021 Apr 26.

Clinical Services and Research, Australian Red Cross Lifeblood, Melbourne, Victoria, Australia.

Background: Fear of blood donation is implicated in vasovagal reactions, donor recruitment, and retention. This study examined the extent to which fear among donors is associated with various donor outcomes in an Australian sample, and whether fear can be addressed on-site to reduce adverse reactions and improve the donation experience.

Study Design And Methods: Six hundred and sixty-four donors (age M = 33. Read More

Transfus Clin Biol 2021 Apr 24. Epub 2021 Apr 24.

Hematology and Transfusion Medicine Center -University of Campinas/Hemocentro-Unicamp, Campinas, São Paulo, Brazil; Universidade São Francisco, Bragança Paulista, São Paulo, Brazil.

Objectives: Blood transfusion is a key intervention for decreasing morbidity and mortality in many cases and, besides its importance, potentially fatal consequences of incompatible transfusion are a great risk to patients. This study evaluated the incidence and specificity of erythrocyte alloantibodies in multi-transfused patients enrolled at an important Regional Blood Center.

Materials/methods: This was a single-center retrospective cohort study that eveluated patients enrolled at a Regional Blood Center in a period of four years. Read More

Analyst 2021 May 26;146(9):2871-2877. Epub 2021 Apr 26.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China. and Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China.

A highly sensitive ELISA is critical for early diagnosis and biomarker discovery of various diseases. Although various ELISA technologies have been developed with high sensitivity, they are limited by poor repeatability, high cost, the dependence on complex equipment and/or a prolonged reaction time. To this end, we developed a fast and ultrasensitive ELISA (termed RELISA) based on rolling circle amplification (RCA) and enzymatic signal amplification. Read More