Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists.
Reprod Toxicol 2018 Feb 16. Epub 2018 Feb 16.
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States. Electronic address:
There continues to be a need to develop in vivo high-throughput screening (HTS) and computational methods to screen chemicals for interaction with the estrogen, androgen, and thyroid pathways and as complements to in vitro HTS assays. This study explored the utility of an embryonic zebrafish HTS approach to identify and classify endocrine bioactivity using phenotypically-anchored transcriptome profiling. Transcriptome analysis was conducted on zebrafish embryos exposed to 25 estrogen-, androgen-, or thyroid-active chemicals at concentrations that elicited adverse malformations or mortality at 120 hours post-fertilization in 80% of animals exposed. Read More