1,894 results match your criteria Toxicity Caffeine

In vitro genotoxic and mutagenic effects of water samples from Sapucaia and Esteio streams (Brazil) under the influence of different anthropogenic activities.

Mutat Res Genet Toxicol Environ Mutagen 2022 Jun 31;878:503484. Epub 2022 Mar 31.

Laboratory of Genetic Toxicology, PPGBioSaúde (Postgraduate Program in Cellular and Molecular Biology Applied to Health), Lutheran University of Brazil (ULBRA), Av. Farroupilha, 8001, Building 22 (4th floor), 92425-900, Canoas, RS, Brazil; Laboratory of Genetic Toxicology, PPGSDH, La Salle University (UniLaSalle), Av. Victor Barreto, 2288, 92010-000, Canoas, RS, Brazil. Electronic address:

Pollution of aquatic ecosystems is associated with the discharge of mainly industrial and urban effluents, which may cause damage to public health. This study aims to evaluate the cytotoxic, genotoxic, and mutagenic potential of surface water samples under the influence of different anthropogenic effluents in a human-derived liver cell line (HepG2). Samples were collected in Esteio and Sapucaia streams (Rio Grande do Sul; Brazil), which flow into the Sinos River, a source of water supply for more than one million people. Read More

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Comparison of cellular mechanisms induced by pharmaceutical exposure to caffeine and its combination with salicylic acid in mussel Mytilus galloprovincialis.

Environ Toxicol Pharmacol 2022 Jul 19;93:103888. Epub 2022 May 19.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy. Electronic address:

Urban and hospital-sourced pharmaceuticals are continuously discharged into aquatic environments, threatening biota. To date, their impact as single compounds has been widely investigated, whereas few information exists on their effects as mixtures. We assessed the time-dependent biological impact induced by environmental concentrations of caffeine alone (CAF; 5 ng/L to 10 µg/L) and its combination with salicylic acid (CAF+SA; 5 ng/L+0. Read More

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Prenatal caffeine exposure induced renal developmental toxicity and transgenerational effect in rat offspring.

Food Chem Toxicol 2022 Jul 11;165:113082. Epub 2022 May 11.

Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, 430071, China; Hubei Provincial Key Laboratory of Developmentally Originated Disorder, Wuhan, 430071, China. Electronic address:

Epidemiological studies revealed that prenatal caffeine exposure (PCE) is associated with adverse gestational outcomes and susceptibility to chronic diseases in offspring, yet the effects of PCE on glomerulosclerosis susceptibility in adult female offspring and its intergenerational transmission remain to be further investigated. Here, we found that PCE caused fetal kidney dysplasia and glomerulosclerosis of the female offspring. Besides, the kidney of F1 offspring in PCE group exhibited the "low expressional programming of AT2R" and "GC-IGF1 programming" alteration. Read More

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Caffeine Enhances the Toxicity of Platinum-Based Drugs at the Molecular Level Even Outside of the Intracellular Environment: A Single-Molecule Force Spectroscopy Study.

J Phys Chem B 2022 May 20;126(17):3291-3299. Epub 2022 Apr 20.

Departamento de Física, Universidade Federal de Viçosa, Viçosa 36570-900, Minas Gerais, Brazil.

It is well reported in the literature that caffeine, the most consumed alkaloid around the world, enhances the anticancer effects of the drug cisplatin by inhibiting DNA repair by the cellular machinery. Here, we perform single-molecule force spectroscopy assays with optical tweezers to show that caffeine enhances the toxicity not only of cisplatin but also of various different platinum-based drugs already at the molecular level, using samples containing only double-stranded (ds)DNA, platinum drugs, and the alkaloid in a simple phosphate buffer, that is, completely out of the complex environment found inside real living cells. In fact, our results show that caffeine acts as an allosteric catalyst which increases the effective equilibrium binding constant between DNA and the platinum drugs, also interfering in the cooperativity of the binding reactions. Read More

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Caffeine Role in the Age-Related Neurodegenerative Diseases, A Review.

Miroslav Pohanka

Mini Rev Med Chem 2022 Apr 13. Epub 2022 Apr 13.

Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove CZ-50001, Czech Republic.

Caffeine, a simple purine alkaloid with the proper chemical name 1,3,7-trimethylpurine-2,6-dione, is an abundant compound contained in beverages like coffee, food and drugs. It interacts with various pathways of which antagonism of adenosine receptors is the most significant but the other physiological pathways can be influenced by caffeine as well. Interaction with glutamate and dopamine neurotransmission pathways, competition with other substrates on cytochrome P450, non-competitive inhibition of acetylcholinesterase, blocking of nicotinic acetylcholine receptor and competitive inhibition of cyclic nucleotide phosphodiesterase can be mentioned. Read More

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Organic micropollutants, heavy metals and pathogens in anaerobic digestate based on food waste.

J Environ Manage 2022 Jul 5;313:114997. Epub 2022 Apr 5.

Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences (SLU), SE-75007, Uppsala, Sweden.

Anaerobic digestate based on food waste is increasingly used as fertilizer in food production. This study examined the characteristics of anaerobic digestate based on food waste from three biogas plants in Sweden. The characterization included measurements of heavy metals (n = 7), chemicals of emerging concern (CECs), such as currently used drugs and pesticides (n = 133), and an extended range of food-borne pathogens, including two notable sporeformers and some widespread antibiotic-resistant bacteria. Read More

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A novel in vitro 3D model of the human bone marrow to bridge the gap between in vitro and in vivo genotoxicity testing.

Mutagenesis 2022 05;37(2):112-129

Department of Applied Sciences, University of the West of England, Bristol, Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, United Kingdom.

The regulatory 2D in vitro micronucleus (MN) assay is part of a battery of tests, used to test for genotoxicity of new and existing compounds before they are assessed in vivo (ICH S2). The 2D MN assay consists of a monolayer of cells, whereas the in vivo bone marrow (BM) setting comprises a multicellular environment within a three-dimensional extracellular matrix. Although the in vitro MN assay follows a robust protocol set out by the Organisation for Economic Co-operation and Development (OECD) to comply with regulatory bodies, some compounds have been identified as negative genotoxicants within the in vitro MN assay but marginally positive when assessed in vivo. Read More

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Commercial and Instant Coffees Effectively Lower Aβ1-40 and Aβ1-42 in N2a/APPswe Cells.

Front Nutr 2022 15;9:850523. Epub 2022 Mar 15.

Department of Pharmaceutical Sciences, Taneja College of Pharmacy, University of South Florida, Tampa, FL, United States.

Background: Alzheimer's disease (AD) is a multifactorial neurological disease with neurofibrillary tangles and neuritic plaques as histopathological markers. Due to this, although AD is the leading cause of dementia worldwide, clinical AD dementia cannot be certainly diagnosed until neuropathological post-mortem evaluation. Coffee has been reported to have neurologically protective factors, particularly against AD, but coffee brand and type have not been taken into consideration in previous studies. Read More

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Differential expression of placental 11β-HSD2 induced by high maternal glucocorticoid exposure mediates sex differences in placental and fetal development.

Sci Total Environ 2022 Jun 5;827:154396. Epub 2022 Mar 5.

Department of Pharmacology, Basic Medical School of Wuhan University, 185 Donghu Road, Wuchang District, Wuhan 430071, China; Department of Obstetrics and Gynaecology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuchang District, Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan 430071, China. Electronic address:

A variety of adverse environmental factors during pregnancy cause maternal chronic stress. Caffeine is a common stressor, and its consumption during pregnancy is widespread. Our previous study showed that prenatal caffeine exposure (PCE) increased maternal blood glucocorticoid levels and caused abnormal development of offspring. Read More

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Will extreme weather events influence the toxic impacts of caffeine in coastal systems? Comparison between two widely used bioindicator species.

Chemosphere 2022 Jun 23;297:134069. Epub 2022 Feb 23.

Department of Biology & CESAM, University of Aveiro, 3810-193, Aveiro, Portugal. Electronic address:

In the recent years, marine heatwaves (MHWs) have caused devastating impacts on marine life. The understanding of the combined effects of these extreme events and anthropogenic pollution is a vital challenge. In particular, the combined effect of MHWs on the toxicity of pharmaceuticals to aquatic life remains unclear. Read More

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Caffeine and MDMA (Ecstasy) Exacerbate ER Stress Triggered by Hyperthermia.

Int J Mol Sci 2022 Feb 10;23(4). Epub 2022 Feb 10.

Molecular Mechanisms of Cellular Stress and Inflammation Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.

Drugs of abuse can cause local and systemic hyperthermia, a known trigger of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). Another trigger of ER stress and UPR is ER calcium depletion, which causes ER exodosis, the secretion of ER-resident proteins. In rodent models, club drugs such as 3,4-methylenedioxymethamphetamine (MDMA, 'ecstasy') can create hyperthermic conditions in the brain and cause toxicity that is affected by the environmental temperature and the presence of other drugs, such as caffeine. Read More

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February 2022

Paraxanthine Supplementation Increases Muscle Mass, Strength, and Endurance in Mice.

Nutrients 2022 Feb 20;14(4). Epub 2022 Feb 20.

Radiant Research Services Pvt. Ltd., Bangalore 560058, India.

Paraxanthine is a natural dietary ingredient and the main metabolite of caffeine in humans. Compared to caffeine, paraxanthine exhibits lower toxicity, lesser anxiogenic properties, stronger locomotor activating effects, greater wake promoting properties, and stronger dopaminergic effects. The purpose of this study was to evaluate the potential beneficial effects of paraxanthine supplementation on muscle mass, strength, and endurance performance in comparison to the control and other ingredients commonly used by athletes: L-theanine, alpha-GPC, and taurine. Read More

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February 2022

Predicting nonlinear relationships between external and internal concentrations with physiologically based pharmacokinetic modeling.

Toxicol Appl Pharmacol 2022 04 15;440:115922. Epub 2022 Feb 15.

U.S. Environmental Protection Agency, Office of Pesticide Programs, Durham, NC, USA. Electronic address:

Although external concentrations are more readily quantified and often used as the metric for regulating and mitigating exposures to environmental chemicals, the toxicological response to an environmental chemical is more directly related to its internal concentrations than the external concentration. The processes of absorption, distribution, metabolism, and excretion (ADME) determine the quantitative relationship between the external and internal concentrations, and these processes are often susceptible to saturation at high concentrations, which can lead to nonlinear changes in internal concentrations that deviate from proportionality. Using generic physiologically-based pharmacokinetic (PBPK) models, we explored how saturable absorption or clearance influence the shape of the internal to external concentration (IEC) relationship. Read More

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Protective Effects of Green Tea Supplementation against Lead-Induced Neurotoxicity in Mice.

Molecules 2022 Feb 1;27(3). Epub 2022 Feb 1.

Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

The use of natural products as therapeutic agents is rapidly growing recently. In the current study, we investigated the protective effects of green tea supplementation on lead-induced toxicity in mice. Forty albino mice were divided into four groups as follows: A: control group; B: green tea receiving group; C: lead-intoxicated group; and D: lead-intoxicated group supplemented with green tea. Read More

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February 2022

Investigation of potential behavioral and physiological effects of caffeine on D. magna.

Environ Sci Pollut Res Int 2022 Jun 29;29(28):43237-43250. Epub 2022 Jan 29.

Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.

With the ever-increasing consumption of pharmaceutical compounds, their presence in the environment is now an undisputable reality. The majority of these compounds are released into the wild after their therapeutic use, as biotransformation products or in their original form. The presence of this class of compounds in the environment, due to their biological properties, can exert effects on non-target organisms, with adverse consequences. Read More

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Neurotoxic, biotransformation, oxidative stress and genotoxic effects in Astyanax altiparanae (Teleostei, Characiformes) males exposed to environmentally relevant concentrations of diclofenac and/or caffeine.

Environ Toxicol Pharmacol 2022 Apr 29;91:103821. Epub 2022 Jan 29.

Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Ecotoxicología Acuática and IBBEA, CONICET-UBA. Ciudad Universitaria, Buenos Aires, Argentina.

The present study evaluated neurotoxic, biotransformation, genotoxic and antioxidant responses to relevant environmental concentrations of diclofenac (0.4 μg L) and caffeine (27.5 μg L), separate and combined, in adult males of the freshwater fish Astyanax altiparanae after a subchronic exposure (14 days). Read More

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A large scale mass spectrometry-based histone screening for assessing epigenetic developmental toxicity.

Sci Rep 2022 01 24;12(1):1256. Epub 2022 Jan 24.

ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.

Toxicoepigenetics is an emerging field that studies the toxicological impact of compounds on protein expression through heritable, non-genetic mechanisms, such as histone post-translational modifications (hPTMs). Due to substantial progress in the large-scale study of hPTMs, integration into the field of toxicology is promising and offers the opportunity to gain novel insights into toxicological phenomena. Moreover, there is a growing demand for high-throughput human-based in vitro assays for toxicity testing, especially for developmental toxicity. Read More

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January 2022

Individual Radiosensitivity as a Risk Factor for the Radiation-Induced Acute Radiodermatitis.

Life (Basel) 2021 Dec 23;12(1). Epub 2021 Dec 23.

Biomedical Physics Laboratory, National Cancer Institute, LT-08660 Vilnius, Lithuania.

Background: Up to 95% of irradiated patients suffer from ionizing radiation (IR) induced early skin reaction, acute radiation dermatitis (ARD). Some experts think that additional skin hydration can reduce acute skin reactions. Individual radiosensitivity (IRS) determined from lymphocytes may help to predict acute radiation toxicity. Read More

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December 2021

A 10-step framework for use of read-across (RAX) in next generation risk assessment (NGRA) for cosmetics safety assessment.

Regul Toxicol Pharmacol 2022 Mar 4;129:105094. Epub 2022 Jan 4.

Cosmetics Europe, 40 Avenue Hermann-Debroux, 1160, Brussels, Belgium. Electronic address:

This paper presents a 10-step read-across (RAX) framework for use in cases where a threshold of toxicological concern (TTC) approach to cosmetics safety assessment is not possible. RAX builds on established approaches that have existed for more than two decades using chemical properties and in silico toxicology predictions, by further substantiating hypotheses on toxicological similarity of substances, and integrating new approach methodologies (NAM) in the biological and kinetic domains. NAM include new types of data on biological observations from, for example, in vitro assays, toxicogenomics, metabolomics, receptor binding screens and uses physiologically-based kinetic (PBK) modelling to inform about systemic exposure. Read More

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Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model.

Environ Sci Pollut Res Int 2022 Apr 4;29(19):27862-27874. Epub 2022 Jan 4.

Department of Biology, Faculty of Science and Art, Giresun University, Giresun, Turkey.

Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI)-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicity, and biochemical parameters as well as meristematic cell injuries in the roots were investigated. Read More

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Solketal Removal from Aqueous Solutions Using Activated Carbon and a Metal-Organic Framework as Adsorbents.

Materials (Basel) 2021 Nov 13;14(22). Epub 2021 Nov 13.

INAMAT2, Departamento de Ciencias, Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain.

The worldwide rise in biodiesel production has generated an excess of glycerol, a byproduct of the process. One of the most interesting alternative uses of glycerol is the production of solketal, a bioadditive that can improve the properties of both diesel and gasoline fuels. Even with its promising future, not much research has been performed on its toxicity in aqueous environments. Read More

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November 2021

Sublethal effects of environmental concentrations of caffeine on a neotropical freshwater fish.

Ecotoxicology 2022 Jan 13;31(1):161-167. Epub 2021 Nov 13.

Programa de Pós-graduação em Ecologia - UFJF, Instituto de Ciências Biológicas - ICB, Campus Universitário, Bairro São Pedro, CEP 36036-900, Juiz de Fora, Minas Gerais, Brazil.

Caffeine is a contaminant frequently detected in water bodies. Growth trends in both human population and caffeine consumption per capita are expected to exacerbate the occurrence of caffeine in freshwaters. Yet the effects of caffeine on native fish fauna are poorly understood. Read More

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January 2022

Neurochemical and Behavioral Consequences of Ethanol and/or Caffeine Exposure: Effects in Zebrafish and Rodents.

Curr Neuropharmacol 2022 Mar;20(3):560-578

Department of Biology and Center for Neuroscience and Behavior, American University, Washington, DC 20016, USA.

Zebrafish are increasingly being utilized to model the behavioral and neurochemical effects of pharmaceuticals and, more recently, pharmaceutical interactions. Zebrafish models of stress establish that both caffeine and ethanol influence anxiety, though few studies have implemented coadministration to assess the interaction of anxiety and reward-seeking. Caffeine exposure in zebrafish is teratogenic, causing developmental abnormalities in the cardiovascular, neuromuscular, and nervous systems of embryos and larvae. Read More

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Quercetin Alleviates Red Bull Energy Drink-Induced Cerebral Cortex Neurotoxicity via Modulation of Nrf2 and HO-1.

Oxid Med Cell Longev 2021 31;2021:9482529. Epub 2021 Oct 31.

Department of Anatomy and Embryology, Faculty of Medicine, Kasr Al-Ainy, Cairo University, Cairo, Egypt.

The current work was aimed at evaluating the ameliorative role of quercetin (QR) on the possible toxicity of Red Bull energy drink (RB-Ed) in the cerebral cortex of rats. To achieve the goal, the rats were allocated into 4 groups. The first group received distilled water as control. Read More

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February 2022

Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy.

Neurobiol Dis 2021 12 6;161:105547. Epub 2021 Nov 6.

INMED, INSERM, Aix-Marseille Univ, France.. Electronic address:

Systemic pilocarpine treatment is one of the most reliable means of inducing temporal lobe epilepsy (TLE). However, the traditional pilocarpine injection protocol using mice was associated with a high death rate, possibly because of cardiorespiratory collapse following status epilepticus (SE). To prevent this, we developed a modified procedure of pilocarpine SE induction, which included a single injection of a moderate dose of caffeine during the induction phase. Read More

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December 2021

Green coffee extract attenuates Parkinson's-related behaviors in animal models.

An Acad Bras Cienc 2021 1;93(suppl 4):e20210481. Epub 2021 Nov 1.

Universidade Federal do ABC, Centro de Ciências Naturais e Humanas, Rua Arcturus, 03, 09606-070 São Bernardo do Campo, SP, Brazil.

Epidemiological studies have shown an inverse association between coffee consumption and the development of Parkinson's disease (PD). The effects of the oral treatment with green (non-roasted) coffee extracts (CE, 100 or 400 mg/kg) and caffeine (31.2 mg/kg) were evaluated on catalepsy induced by haloperidol in mice, and unilateral 6-OHDA lesion of medial forebrain bundle (MFB) or striatum in rats. Read More

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November 2021

Interaction of Energy Drinks with Prescription Medication and Drugs of Abuse.

Pharmaceutics 2021 Sep 22;13(10). Epub 2021 Sep 22.

Clinical Pharmacology Unit, Hospital Universitari Germans Trias i Pujol, Institut de Recerca Germans Trias i Pujol (HUGTiP-IGTP), 08916 Badalona, Spain.

In recent years, the consumption of energy drinks (EDs) has become increasingly popular, especially among adolescents. Caffeine, a psychostimulant, is the main compound of EDs which also contain other substances with pharmacological effects. This review aims to compile current evidence concerning the potential interactions between EDs, medicines, and drugs of abuse as they are frequently consumed in combination. Read More

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September 2021

Neuromotor activity inhibition in zebrafish early-life stages after exposure to environmental relevant concentrations of caffeine.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2021 18;56(12):1306-1315. Epub 2021 Oct 18.

Faculdade de Tecnologia, Universidade Estadual de Campinas, UNICAMP, Limeira, São Paulo, Brasil.

Caffeine (CAF), a neuroactive compound, has been found in surface waters at concentrations ranging from few nanograms up to micrograms and may induce adverse effects in aquatic vertebrates. Thus, the aim of this study was to evaluate the potential of CAF in affecting fish early-life stages in a wide concentration range, including occurring levels in surface waters. Specimens of zebrafish in early-life stages were exposed to CAF for 168 h and survival, developmental alterations, locomotor activity and acetylcholinesterase activity were evaluated. Read More

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December 2021

An assessment of mutagenicity, genotoxicity, acute-, subacute and subchronic oral toxicity of paraxanthine (1,7-dimethylxanthine).

Food Chem Toxicol 2021 Dec 28;158:112579. Epub 2021 Sep 28.

LSRO Solutions, LLC, 2286 Dunster Lane, Rockviell, MD, 20845, USA.

Paraxanthine or 1,7-dimethylxanthine is a natural dietary component and the main metabolite of caffeine in humans. A battery of toxicological studies was conducted in accordance with international guidelines to investigate mutagenicity, genotoxicity and acute and repeated-dose oral toxicity in rats of synthetic paraxanthine (ENFINITY™, Ingenious Ingredients, L.P. Read More

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December 2021