Graefes Arch Clin Exp Ophthalmol 2022 Apr 23. Epub 2022 Apr 23.
Centre for Ocular Regeneration (CORE), Prof. Brien Holden Eye Research Centre (BHERC), LV Prasad Eye Institute, Road No.2, Banjara Hills, Hyderabad, 500034, Telangana, India.
Purpose: To study the cytokeratin profile and keratinization-related gene expression in keratinized lid margins of chronic Stevens-Johnson syndrome (SJS) patients.
Methods: Posterior eyelid margins from 24 chronic SJS patients undergoing mucous membrane grafting and six healthy margins (orbital exenteration, fresh body donors) were studied using immunofluorescence staining (CK10, CK1, filaggrin, transglutaminase 1 (TGM1), (CK19, MUC5AC)) and quantitative PCR (keratinization-related genes-HBEGF, KGF, EGF, TGFα, TGFβ, and TNFα). The staining and gene expression were studied separately in the lid margin epidermis (LME) and lid margin conjunctiva (LMC). Read More