J Invest Dermatol 2021 Apr 1. Epub 2021 Apr 1.
Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA; Department of Anesthesiology, Center for the Study of Itch & Sensory Disorders, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA.
Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of UV irradiation‒induced itch are poorly understood because of a lack of experimental animal models of sunburn itch. In this study, we established a sunburn-related mouse model and found that broad-band UVB irradiation elicited scratching but not wiping behavior in mice. Using a combination of live-cell calcium ion imaging and quantitative RT-PCR on dorsal root ganglion neurons, H&E staining, immunofluorescence staining of skin preparations, and behavioral testing, in combination with genetic and pharmacological approaches, we showed that TRPV1-positive dorsal root ganglion neurons but not mast cells are involved in broad-band UVB irradiation‒induced itch. Read More