Metaperiodate deglycosylation of Strongyloides venezuelensis larvae: immunochemical characterization and antigen production for human strongyloidiasis diagnosis.
- Henrique Tomaz Gonzaga,
- Daniela da Silva Nunes,
- Vanessa da Silva Ribeiro,
- Nágilla Daliane Feliciano,
- Jair Pereira da Cunha-Junior,
- Julia Maria Costa-Cruz
Acta Trop 2018 Feb 15. Epub 2018 Feb 15.
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil. Electronic address:
Strongyloidiasis is an important helminthiasis affecting million people worldwide. The aim of this study was to use sodium metaperiodate (MP) treatment to immunochemically characterize Strongyloides venezuelensis filariform larvae and use MP-treated heterologous antigen to detect IgG and subclasses in serum. Samples from individuals with definitive diagnosis of strongyloidiasis (n = 50), other parasitic diseases (n = 60) and negative endemic (n = 50) were tested. Read More