6,898 results match your criteria Streptococcus Group B Infections


Efficacy of live attenuated vaccine derived from the Streptococcus agalactiae on the immune responses of Oreochromis niloticus.

Fish Shellfish Immunol 2019 Apr 19. Epub 2019 Apr 19.

School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.

Streptococcus agalactiae species have been recognized as the main pathogen causing high mortality in fish leading to significant worldwide economical losses to the aquaculture industries. Vaccine development has become a priority in combating multidrug resistance in bacteria; however, there is a lack of commercial live attenuated vaccine (LAV) against S. agalactiae in Malaysia. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.052DOI Listing

Fulminant early-onset neonatal sepsis due to : Case report and review of the literature.

Pediatr Rep 2019 Feb 27;11(1):7953. Epub 2019 Mar 27.

Department of Neonatology.

is a rare cause of neonatal sepsis, and it is associated with significant morbidity and a very high fatality rate. The infection is usually acquired intrapartum, from the colonization of the maternal genital tract. Most affected neonates have an early-onset presentation of symptoms, usually within the first 48 hours after birth, which is similar to other causes of neonatal sepsis such as or . Read More

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https://www.pagepress.org/journals/index.php/pr/article/view
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http://dx.doi.org/10.4081/pr.2019.7953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452222PMC
February 2019
1 Read

Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of .

Biosci Rep 2019 Apr 15. Epub 2019 Apr 15.

Department of Clinical Laboratory Medicine, the Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road,Liwan District, Guangzhou, Georgia, 510150, China

 is a major pathogenic bacterium causing perinatal infections in humans. In this study, a novel real-time fluorescence loop-mediated isothermal amplification technology was successfully developed and evaluated for the detection of  in a single reaction. Six specific primers were designed to amplify the corresponding six regions of fbs B gene of , using Bst DNA polymerase with DNA strand displacement activity at a constant temperature for 60 minutes. Read More

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http://dx.doi.org/10.1042/BSR20190383DOI Listing

Exploring Bacterial Resistance in Northern Oman, a Foundation for Implementing Evidence-Based Antimicrobial Stewardship Program.

Int J Infect Dis 2019 Apr 5. Epub 2019 Apr 5.

Philadelphia College of Pharmacy at University of the Sciences, Philadelphia, PA, USA.

Background: Increasing rate of resistant infections is a challenge to healthcare negatively impacting therapeutic and financial outcomes. Targeted antimicrobial stewardship interventions are needed to counteract this global crisis. On large scale, we sought to identify the prevalence of resistant pathogens and their susceptibility pattern in Northern Oman. Read More

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http://dx.doi.org/10.1016/j.ijid.2019.04.004DOI Listing
April 2019
4 Reads

Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model.

Microb Pathog 2019 Mar 30;131:47-52. Epub 2019 Mar 30.

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.

This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 10 to 10 CFU/mL, and immersion (IM) route from 10 to 10 CFU/mL. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.03.034DOI Listing

Universal gestational screening for Streptococcus agalactiae colonization and neonatal infection - A systematic review and meta-analysis.

J Infect Public Health 2019 Mar 30. Epub 2019 Mar 30.

Health Sciences Faculty, University of Western Sao Paulo, 700, Jose Bongiovani St., Presidente Prudente, Sao Paulo, Brazil. Electronic address:

The review aimed to analyze the evidence of the correlation between universal screening for Streptococcus agalactiae colonization in pregnant women and early onset Group B neonatal infection. The research followed the descriptors "screening", "pregnancy", "Streptococcus agalactiae" and "neonatal infections" on the Pubmed, scielo and LILACS databases, for studies published in English between January 1st, 2008 and April 24th, 2018. A total of 200 articles were found, of which 198 were excluded. Read More

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http://dx.doi.org/10.1016/j.jiph.2019.03.004DOI Listing
March 2019
1 Read

Economic and epidemiological impact of different intervention strategies for subclinical and clinical mastitis.

Prev Vet Med 2019 May 6;166:78-85. Epub 2019 Mar 6.

Epidemiology Group, National Veterinary Institute, Technical University of Denmark, Kgs. Lyngby, Denmark; Section of Animal Welfare and Disease Control, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, Copenhagen University, Frederiksberg, Denmark.

The objective of this study was to evaluate and compare different combinations of intervention strategies for contagious or opportunistic subclinical and clinical intramammary infections (IMI). We simulated two different Danish dairy cattle herds with ten different intervention strategies focusing on cow-specific treatment or culling, including three baseline strategies without subclinical interventions. In one herd, the main causative pathogen of IMI was Staphylococcus (S. Read More

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http://dx.doi.org/10.1016/j.prevetmed.2019.03.001DOI Listing

Subspecies Typing of Based on Ribosomal Subunit Protein Mass Variation by MALDI-TOF MS.

Front Microbiol 2019 11;10:471. Epub 2019 Mar 11.

Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute (Swiss TPH) Basel, Basel, Switzerland.

A ribosomal subunit protein (rsp)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed for fast subspecies-level typing of (Group B , GBS), a major cause of neonatal sepsis and meningitis. A total of 796 GBS whole genome sequences, covering the genetic diversity of the global GBS population, were used to predict molecular mass variability of 28 rsp and to identify unique rsp mass combinations, termed "rsp-profiles". The established GBS typing scheme was validated by MALDI-TOF MS analysis of GBS isolates at two independent research sites in Europe and South East Asia. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421976PMC
March 2019
1 Read

Conserved and specific features of Streptococcus pyogenes and Streptococcus agalactiae transcriptional landscapes.

BMC Genomics 2019 Mar 22;20(1):236. Epub 2019 Mar 22.

Ecology and Evolution of Resistance to Antibiotics, Institut Pasteur-APHP-Université Paris Saclay, UMR3525 CNRS, Paris, France.

Background: The human pathogen Streptococcus pyogenes, or group A Streptococcus, is responsible for mild infections to life-threatening diseases. To facilitate the characterization of regulatory networks involved in the adaptation of this pathogen to its different environments and their evolution, we have determined the primary transcriptome of a serotype M1 S. pyogenes strain at single-nucleotide resolution and compared it with that of Streptococcus agalactiae, also from the pyogenic group of streptococci. Read More

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http://dx.doi.org/10.1186/s12864-019-5613-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431027PMC

Group B ().

Microbiol Spectr 2019 03;7(2)

Division of Pediatric Infectious Diseases, Emory University School of Medicine and Children's Healthcare of Atlanta, Atlanta, GA 30322.

Invasive disease due to group B infection () results in a wide spectrum of clinical disease. In North America, serotypes Ia, Ib, II, III, and V are most frequently associated with invasive disease. Group B remains a continuing source of morbidity and mortality in high-risk populations, including pregnant women, neonates, and the elderly; an increasing incidence of invasive disease has been observed in nonpregnant adults. Read More

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http://dx.doi.org/10.1128/microbiolspec.GPP3-0007-2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432937PMC
March 2019
3 Reads

[Analysis of screening strategy of group B streptococcus in the third trimester and its influence on pregnancy outcome].

Zhonghua Fu Chan Ke Za Zhi 2019 Mar;54(3):154-159

Department of Obstetrics and Gynecology, Bao'an Maternal and Child Health Hospital, Jinan University, Shenzhen 518106, China.

To investigate the screening strategy of group B streptococcus (GBS) in the reproductive tract of women in the third trimester and analyze its impact on pregnancy outcome. A total of 85 461 pregnant women in 35-37 weeks of gestation from Bao'an Maternity and Child Health Hospital, Jinan University from January 2011 to June 2018 were enrolled. They were divided into 3 periods according to different GBS screening strategies, the unscreened period included 31 384 cases (36. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0529-567x.2019.03.003DOI Listing
March 2019
3 Reads

Development of attenuated erythromycin-resistant Streptococcus agalactiae vaccine for tilapia (Oreochromis niloticus) culture.

J Fish Dis 2019 May 20;42(5):693-701. Epub 2019 Mar 20.

State Key Laboratory of Biocontrol/Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.

Streptococcus agalactiae is an important pathogen in fish, causing great losses of intensive tilapia farming. To develop a potential live attenuated vaccine, a re-attenuated S. agalactiae (named TFJ-ery) was developed from a natural low-virulence S. Read More

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http://dx.doi.org/10.1111/jfd.12977DOI Listing

Purulent Pericarditis in Sickle Cell Disease Due to Streptococcus agalactiae; a Unique Case Report and Literature Review.

Hemoglobin 2019 Mar 18:1-3. Epub 2019 Mar 18.

a Downstate College of Medicine, Department of Medicine, Division of Hematology-Oncology , State University of New York , Brooklyn , NY , USA.

Purulent pericarditis is a localized infection with a thick, fibrinous hypercellular exudate and is historically associated with a high mortality. We describe a case of purulent pericarditis due to Streptococcus agalactiae (S. agalactiae) in a 30-year-old woman with sickle cell disease who presented with fever, dyspnea, and S. Read More

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http://dx.doi.org/10.1080/03630269.2019.1579736DOI Listing
March 2019
2 Reads
0.955 Impact Factor

[Pelvic inflammatory diseases: Microbiologic diagnosis - CNGOF and SPILF Pelvic Inflammatory Diseases Guidelines].

Gynecol Obstet Fertil Senol 2019 Mar 13. Epub 2019 Mar 13.

Infections humaines à mycoplasmes et chlamydiae, USC EA 3671, Institut national de la recherche agronomique, université Bordeaux, 33000 Bordeaux, France; Centre national de référence des infections sexuellement transmissibles bactériennes, CHU de Bordeaux, 33000 Bordeaux, France; Laboratoire de bactériologie, groupe hospitalier Pellegrin, CHU de Bordeaux, 33000 Bordeaux, France.

Objectives: To determine the microorganisms potentially involved in pelvic inflammatory diseases (PIDs) and the different diagnostic methods of PID.

Methods: PubMed and International Guidelines search.

Results: PIDs have various microbial causes. Read More

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http://dx.doi.org/10.1016/j.gofs.2019.03.007DOI Listing
March 2019
2 Reads

Evaluation of BACT/ALERT® VIRTUO®, BACT/ALERT 3D®, and BACTEC™ FX Automated Blood Culture Systems for Detection of Microbial Pathogens Using Simulated Human Blood Samples.

Front Microbiol 2019 19;10:221. Epub 2019 Feb 19.

Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome, Italy.

Blood culture (BC) is still the standard for diagnosing bloodstream infections (BSIs), especially those caused by bacteria and fungi. Infection-complicating sepsis or septic shock often occurs at BSI onset, making necessary to improve the diagnostic yield of positive BCs. Among the BC systems currently available, the BACT/ALERT® VIRTUO® (VIRTUO) system has been developed to shorten time to detection (TTD) of positive BCs. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389693PMC
February 2019
3 Reads

Purification and functional characterization of serum transferrin from Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2019 May 1;88:36-46. Epub 2019 Mar 1.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China. Electronic address:

Transferrin (TF), an iron-binding multifunctional protein, could participate in the iron-withholding strategy, an effective antimicrobial defense mechanism in innate immunity, and is involved in host defense against pathogenic infection. In this study, a TF homologue (OnTF) was purified from serum of Nile tilapia (Oreochromis niloticus) through a two-step affinity chromatography, and characterized its antibacterial function and the role in inflammatory response. The identification by mass spectrometry showed that peptide sequence of the purified OnTF was highly consistent with its amino acids sequence, containing two conserved iron binding lobes: N-lobe and C-lobe. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193013
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http://dx.doi.org/10.1016/j.fsi.2019.02.060DOI Listing
May 2019
6 Reads

Lyn is involved in host defense against S. agalactiae infection and BCR signaling in Nile tilapia (Oreochromis niloticus).

Dev Comp Immunol 2019 Jul 26;96:1-8. Epub 2019 Feb 26.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China. Electronic address:

Lyn, a member of Src protein kinase family, plays a crucial role in immune reactions against pathogenic infection. In this study, Lyn from Nile tilapia (Oreochromis niloticus) (OnLyn) was identified and characterized at expression pattern against bacterial infection, and regulation function in BCR signaling. The open reading frame of OnLyn contained 1536 bp of nucleotide sequence encoded a protein of 511 amino acids. Read More

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http://dx.doi.org/10.1016/j.dci.2019.02.013DOI Listing
July 2019
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Efficacy of Injectable and Immersion Polyvalent Vaccine against Streptococcal Infections in Broodstock and Offspring of Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2019 May 23;88:293-300. Epub 2019 Feb 23.

Department of Infectious Disease, Faculty of Veterinary Medicine, Cairo University, Egypt. Middle East for Veterinary Vaccines Company, Second Industrial Area, ElSalihya El-Gededa, El-Sharkia, 44671, Egypt. Electronic address:

A vaccine against streptococcosis, lactococcosis and enterococcosis in tilapia was formulated, ME-VAC Aqua Strept, as a polyvalent inactivated vaccine containing Streptococcus agalactiae, S. iniae, Lactococcus garvieae and Enterococcus faecalis along with a nano-particulate adjuvant. Use of ME-VAC Aqua Strept by injection or immersion resulted in an improved non-specific and adaptive immunity of broodstock and offspring. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.042DOI Listing
May 2019
1 Read

Identification and functional characterization of a c-type lysozyme from Fenneropenaeus penicillatus.

Fish Shellfish Immunol 2019 May 22;88:161-169. Epub 2019 Feb 22.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, China. Electronic address:

Lysozyme is an important defense molecule of the innate immune system and possess high antimicrobial activities. In this study, a full-length c-type lysozyme cDNA (Fplysc) was cloned and characterized from Fenneropenaeus penicillatus. The cDNA contains an open reading frame of 477 bp encoding 158 amino acids, with 53-94% identity with those of other crustaceans. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193011
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http://dx.doi.org/10.1016/j.fsi.2019.02.043DOI Listing
May 2019
6 Reads

1-Amino-2'-fucosyllactose inhibits biofilm formation by Streptococcus agalactiae.

J Antibiot (Tokyo) 2019 Feb 22. Epub 2019 Feb 22.

Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Nashville, TN, 37235, USA.

2'-Fucosyllactose (2'-FL) is a ubiquitous oligosaccharide in human milk. Importantly, this carbohydrate promotes the growth of several strains of Bifidobacteria, a class of beneficial gut commensal, and inhibits epithelial binding of pathogens. In light of these protective effects, we elected to evaluate the potential of 2'-FL to serve as an antibacterial agent against Group B Streptococcus (GBS). Read More

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http://dx.doi.org/10.1038/s41429-019-0151-6DOI Listing
February 2019

Prevalence and capsular type distribution of Streptococcus agalactiae isolated from pregnant women in Namibia and South Africa.

BMC Infect Dis 2019 Feb 20;19(1):179. Epub 2019 Feb 20.

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice, Eastern Cape Province, 5700, South Africa.

Background: Streptococcus agalactiae or Group B Streptococcus (GBS) is the leading cause of neonatal morbidity and mortality resulting in septicaemia, bacteraemia and meningitis. Long term problems in children range from loss of hearing to mental retardation. While Intrapartum Antibiotic Prophylaxis (IAP) has reduced the incidence of S. Read More

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http://dx.doi.org/10.1186/s12879-019-3809-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383256PMC
February 2019
4 Reads

Characterization of Galectin-2 from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection.

Fish Shellfish Immunol 2019 Apr 16;87:737-743. Epub 2019 Feb 16.

College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:

galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal-2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following bacterial infection were also investigated. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.026DOI Listing

A novel hexavalent capsular polysaccharide conjugate vaccine (GBS6) for the prevention of neonatal group B streptococcal infections by maternal immunization.

J Infect Dis 2019 Feb 19. Epub 2019 Feb 19.

Vaccine Research and Development, Pfizer Inc., Pearl River, NY, USA.

Background: Group B streptococcus (GBS) causes serious diseases in newborn infants, often resulting in lifelong neurologic impairments or death. Prophylactic vaccination of pregnant women prior to delivery could provide comprehensive protection, as early and late onset disease and maternal complications potentially could be addressed.

Methods: Capsular polysaccharide conjugate vaccine GBS6 was designed using Whole Genome Sequencing surveillance data of recent, global GBS isolates responsible for invasive neonatal GBS disease. Read More

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http://dx.doi.org/10.1093/infdis/jiz062DOI Listing
February 2019
8 Reads

Evaluation of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for the Identification of Group B Streptococcus.

BMC Res Notes 2019 Feb 14;12(1):85. Epub 2019 Feb 14.

Section of Paediatrics, Department of Medicine, Imperial College London, London, UK.

Objective: Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and sepsis worldwide. Intrapartum antibiotics given to women carrying GBS are an effective means of reducing disease in the first week of life. Rapid and reliable tests are needed to accurately identify GBS from these women for timely intrapartum antibiotic administration to prevent neonatal disease. Read More

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http://dx.doi.org/10.1186/s13104-019-4119-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376729PMC
February 2019
2 Reads

Defining the Role of the Sht-Family Proteins in Zinc Acquisition and Complement Evasion.

J Bacteriol 2019 Apr 26;201(8). Epub 2019 Mar 26.

ISP, Université de Tours, INRA, UMR1282, Tours, France

is not only part of the human intestinal and urogenital microbiota but is also a leading cause of septicemia and meningitis in neonates. Its ability to cause disease depends upon the acquisition of nutrients from its environment, including the transition metal ion zinc. The primary zinc acquisition system of the pathogen is the Adc/Lmb ABC permease, which is essential for viability in zinc-restricted environments. Read More

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http://dx.doi.org/10.1128/JB.00757-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436357PMC
April 2019
1 Read

[Streptococcus agalactiae in non-pregnant adults. Analysis of 10 years in a University Hospital.]

Rev Fac Cien Med Univ Nac Cordoba 2018 12 12;75(4):255-260. Epub 2018 Dec 12.

Hospital Nacional de Clínicas de Córdoba- Argentina.

Introduction: Streptococcus agalactiae (GBS) is a recognized pathogen associated with infections in newborns and small infants, pregnant women, and puerperal women. In recent years there has been an increase in infections by this agent in non-pregnant adult patients, being more frequent in people over 65 years of age and with some comorbidity.

Methods: retrospective and observational study of samples from non-pregnant adult patients analyzed in the Bacteriology Laboratory of the Hospital Nacional de Clínicas of the city of Córdoba, from January 2008 to December 2017. Read More

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http://dx.doi.org/10.31053/1853.0605.v75.n4.20569DOI Listing
December 2018
1 Read

Sublimate rather than scream.

Authors:
John M Thorp

BJOG 2019 Mar;126(4):433

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http://dx.doi.org/10.1111/1471-0528.15358DOI Listing

Role of Two-Component System Response Regulator in the Antimicrobial Resistance, Virulence, Biofilm Formation, and Stress Response of Group B Streptococcus.

Front Microbiol 2019 23;10:10. Epub 2019 Jan 23.

Department of Microbiology, The Chinese University of Hong Kong, Shatin, Hong Kong.

Group B Streptococcus (GBS; ) is a leading cause of sepsis in neonates and pregnant mothers worldwide. Whereas the hyper-virulent serogroup III clonal cluster 17 has been associated with neonatal disease and meningitis, serogroup III ST283 was recently implicated in invasive disease among non-pregnant adults in Asia. Here, through comparative genome analyses of invasive and non-invasive ST283 strains, we identified a truncated DNA-binding regulator of a two-component system in a non-invasive strain that was homologous to , encoding the response regulator, which was conserved among GBS strains. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2019.00010
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http://dx.doi.org/10.3389/fmicb.2019.00010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351488PMC
January 2019
13 Reads
3.941 Impact Factor

Burden and risk factors of invasive group B Streptococcus disease among neonates in a Chinese maternity hospital.

BMC Infect Dis 2019 Feb 6;19(1):123. Epub 2019 Feb 6.

University of International Business and Economics, 10 Huixing East Road, Beijing, 100029, China.

Background: There is a lack of data regarding the prevalence of invasive group B streptococcus (GBS) infection among neonates in China. This study aimed to investigate the incidence and mortality of invasive GBS infection and to identify the risk factors in our hospital.

Methods: Seventy-four cases admitted between January 2011 and December 2016 was included in this study. Read More

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http://dx.doi.org/10.1186/s12879-018-3660-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366032PMC
February 2019
1 Read

Capsular Type, Sequence Type and Microbial Resistance Factors Impact on DNase Activity of Strains from Human and Bovine Origin.

Eur J Microbiol Immunol (Bp) 2018 Dec 11;8(4):149-154. Epub 2018 Dec 11.

Department of Infectious Diseases, National Institute of Health, Lisbon.

Extracellular deoxyribonucleases (DNases) contribute to the spread of pathogenic bacteria through the evasion from host innate immunity. Our main objective was to evaluate the production of extracellular DNases by human and bovine clinical strains and perform a correlation of genetic lineages and DNase activity with capsular type, genetic determinants, clinical origin (colonization and infection), and host (human or bovine). DNase activity was evaluated by qualitative and quantitative assays for a collection of 406 human ( = 285) and bovine ( = 121) strains. Read More

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http://dx.doi.org/10.1556/1886.2018.00026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348702PMC
December 2018

The activity of commercial antimicrobials, and essential oils and ethanolic extracts of Olea europaea on Streptococcus agalactiae isolated from pregnant women.

BMC Complement Altern Med 2019 Jan 30;19(1):34. Epub 2019 Jan 30.

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice, Eastern Cape Province, 5700, South Africa.

Background: Streptococcus agalactiae also known as Group B Streptococcus (GBS) is a major cause of disease in pregnant women and new born babies where it causes early and late onset disease characterised by sepsis, pneumonia and meningitis. Ten to 37 % of pregnant women in the world are colonised with GBS while intrapartum antibiotic prophylaxis has led to significant reduction in early onset disease. The increase in drug resistant microorganisms has become a major threat. Read More

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http://dx.doi.org/10.1186/s12906-019-2445-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354339PMC
January 2019
4 Reads

Fluoroquinolone-Resistant Streptococcus agalactiae Invasive Isolates Recovered in Argentina.

Microb Drug Resist 2019 Jan 24. Epub 2019 Jan 24.

1 Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología, Biotecnología y Genética, Cátedra de Microbiología, Buenos Aires, Argentina.

Background: Streptococcus agalactiae or group B Streptococcus (GBS) is an important pathogen in neonates and nonpregnant individuals. Epidemiological studies of GBS resistance to fluoroquinolones (FQs) in Latin America are scarce. This study aimed to determine the local prevalence of FQ resistance in the frame of a national, prospective multicenter study of invasive GBS infections and to investigate mechanisms of resistance, serotype distribution, and clonal relationships among resistant isolates. Read More

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http://dx.doi.org/10.1089/mdr.2018.0246DOI Listing
January 2019
1 Read

pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity.

Fish Shellfish Immunol 2019 Apr 19;87:705-713. Epub 2019 Jan 19.

Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Shandong Key Lab for Marine Fishery Biotechnology and Genetic Breeding, Qingdao, 266071, China. Electronic address:

A full-length cDNA encoding phosphatidylinositol 3-kinase regulatory subunit gamma b gene in Nile tilapia (Oreochromis niloticus), termed as On-pik3r3b, was identified and characterized in this study. The sequence analysis demonstrated that the full-length cDNA of On-pik3r3b was 2018 bp, containing a 5' untranslated region (UTR) of 171 bp, an open reading frame (ORF) of 1422 bp and a 3' UTR of 425 bp. Its protein sequence displayed a high degree of identity with other fish. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193000
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http://dx.doi.org/10.1016/j.fsi.2019.01.006DOI Listing
April 2019
17 Reads

A Counterselectable Sucrose Sensitivity Marker Permits Efficient and Flexible Mutagenesis in .

Appl Environ Microbiol 2019 Apr 22;85(7). Epub 2019 Mar 22.

New York University School of Medicine, Department of Pediatrics, New York, New York, USA

(group B [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as single-base-pair polymorphisms. To address this problem, we have developed an efficient and flexible GBS mutagenesis protocol based on sucrose counterselection against levansucrase (SacB) expressed from a temperature-selective shuttle vector. Read More

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http://dx.doi.org/10.1128/AEM.03009-18DOI Listing
April 2019
2 Reads
3.668 Impact Factor

Functional characterization of a mannose-binding lectin (MBL) from Nile tilapia (Oreochromis niloticus) in non-specific cell immunity and apoptosis in monocytes/macrophages.

Fish Shellfish Immunol 2019 Apr 14;87:265-274. Epub 2019 Jan 14.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou 510631, PR China. Electronic address:

Mannose-binding lectin (MBL), a soluble pattern recognition receptor, is able to recognize antigen and participate in non-specific cell immunity, such as regulation of inflammation, migration, opsonization, phagocytosis and killing, which plays an important role in innate immunity. In this study, we have investigated the contributing mechanisms and effects of MBL on the cell immunity of Nile tilapia (Oreochromis niloticus) monocytes/macrophages. The mRNA expression level of OnMBL was significantly up-regulated in monocytes/macrophages after in vitro bacterial infection (Streptococcus agalactiae and Aeromonas hydrophila). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193002
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http://dx.doi.org/10.1016/j.fsi.2019.01.019DOI Listing
April 2019
7 Reads

B cell receptor accessory molecule CD79 gets involved in response against Streptococcus agalactiae infection and BCR signaling in Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2019 Apr 12;87:212-219. Epub 2019 Jan 12.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China. Electronic address:

CD79, composed of two distinct chains called CD79a and CD79b, is a transmembrane protein that forms a B cell antigen receptor with membrane immunoglobulin, and generates a signal following antigen recognition by the B cell receptor. In this study, the CD79a (OnCD79a) and CD79b (OnCD79b) were cloned and identified from Nile tilapia (Oreochromis niloticus). The cDNA of ORF for OnCD79a and OnCD79b are 669 and 627 bp, coding 222 and 208 amino acids, respectively. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.01.012DOI Listing
April 2019
7 Reads

Identification and expression profiling analysis of microRNAs in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae infection.

Fish Shellfish Immunol 2019 Apr 12;87:333-345. Epub 2019 Jan 12.

Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. Electronic address:

MicroRNAs (miRNAs) play vital regulatory roles in various biological processes, including in immune responses. Nile tilapia (Oreochromis niloticus) is an important commercial fish species in China. To identify immune-related miRNAs of O. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.01.018DOI Listing
April 2019
3 Reads

Evaluation of the FilmArray™ Meningitis/Encephalitis panel with focus on bacteria and Cryptococcus spp.

J Microbiol Methods 2019 Feb 10;157:113-116. Epub 2019 Jan 10.

Department of Laboratory Medicine, Clinical Microbiology, University Hospital, Örebro SE-701 85, Sweden; Department of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro SE-701 82, Sweden. Electronic address:

Purpose: Molecular methods provide fast and accurate detection of both bacteria and viruses in the cerebrospinal fluid (CSF) causing infection in the central nervous system (CNS). In the present study we evaluated the bacterial detection performance of the fully automated FilmArray™ Meningitis/Encephalitis (ME) panel (bioMérieux) by comparing it with culture and multiplexed in-house PCR.

Methods: Three sample types were analysed; Contrived samples with known bacterial/fungal concentration (n = 29), clinical samples from patients with verified cause of CNS infection (n = 17) and external quality assessment (EQA) samples (n = 11). Read More

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http://dx.doi.org/10.1016/j.mimet.2019.01.003DOI Listing
February 2019
3 Reads

Potential effect of selective pressure with different β-lactam molecules on the emergence of reduced susceptibility to β-lactams in group B Streptococci.

Microbiol Immunol 2019 Feb 4;63(2):65-76. Epub 2019 Feb 4.

Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.

In this study, the selective potential of group B Streptococcus isolates with reduced penicillin susceptibility (PRGBS) in a neonate-hypervirulent sequence type (ST)17 lineage was investigated by in vitro exposure to β-lactams. After 19 passages of stepwise penicillin exposure, PRGBS with a high penicillin minimum inhibitory concentration MIC (0.5 mg/L), greatly augmented ceftibuten MIC (>512 mg/L), and acquisition of G406D predicted to provide destabilizing effect (ΔΔG 0. Read More

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http://dx.doi.org/10.1111/1348-0421.12667DOI Listing
February 2019
1 Read

Economic appraisal of vaccination against Streptoccocus agalactiae in Nile tilapia farms in Brazil.

Prev Vet Med 2019 Jan 6;162:131-135. Epub 2018 Dec 6.

EpiPlan-Veterinary Epidemiology Laboratory, Faculty of Agronomy and Veterinary Medicine, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, Brazil.

Infection with Streptococcus agalactiae causes mortality and major economic losses in Nile tilapia (Oreochromis niloticus) farming worldwide. In Brazil, serotype strains Ia, Ib and III have been isolated in streptococcosis outbreaks, but serotype Ib is the most prevalent. Vaccination is considered an effective method to prevent economically-important diseases in aquaculture and has been associated with decreased use of antibiotics and improvements in fish survival. Read More

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http://dx.doi.org/10.1016/j.prevetmed.2018.12.003DOI Listing
January 2019
4 Reads

Prevalence, Risk Factors, and Serotype Distribution of Group B Streptococcus Colonization in HIV-Infected Pregnant Women Living in Belgium: A Prospective Cohort Study.

Open Forum Infect Dis 2018 Dec 30;5(12):ofy320. Epub 2018 Nov 30.

Department of Pediatrics, Centre Hospitalier Universitaire Saint-Pierre, Université Libre de Bruxelles (ULB), Brussels, Belgium.

Background: Group B streptococcus (GBS) infection is a leading cause of severe neonatal infection. Maternal GBS carriage during pregnancy is the main risk factor for both early-onset and late-onset GBS disease. High incidence of GBS infection has been reported in HIV-exposed but -uninfected infants (HEU). Read More

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http://dx.doi.org/10.1093/ofid/ofy320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306564PMC
December 2018
3 Reads

Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach.

BMC Genomics 2019 Jan 7;20(1):11. Epub 2019 Jan 7.

AQUACEN - National Reference Laboratory of Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

Background: Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMS) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans.

Results: A total of 1070 protein clusters were identified by nanoLC-UDMS in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). Read More

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https://bmcgenomics.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12864-018-5423-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323687PMC
January 2019
10 Reads

In vitro activity of dihydrofolate reductase inhibitors and other antibiotics against Gram-positive pathogens collected globally between 2004 and 2016.

J Glob Antimicrob Resist 2019 Mar 26;16:236-238. Epub 2018 Oct 26.

Motif BioSciences, Princeton, New Jersey, USA; Rutgers New Jersey Medical School, Trenton, New Jersey, USA. Electronic address:

Objectives: The objective of this study was to determine the in vitro activity of iclaprim and comparator agents against 7618 Gram-positive clinical isolates collected in the periods 2004-2006, 2012-2014 and 2015-2016.

Methods: Antimicrobial susceptibility testing was performed by the broth microdilution method and the minimum inhibitory concentrations (MICs) were interpreted according to Clinical and Laboratory Standards Institute (CLSI) guidelines.

Results: Iclaprim MIC/MIC values were 0. Read More

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http://dx.doi.org/10.1016/j.jgar.2018.10.018DOI Listing

Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?

BMC Infect Dis 2019 Jan 3;19(1). Epub 2019 Jan 3.

R&D Center, Autobio Diagnostics Co. Ltd, Zhengzhou, China.

Background: For a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough.

Methods: To confirm whether a positive CAMP test is a requirement for the identification of S. Read More

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https://bmcinfectdis.biomedcentral.com/articles/10.1186/s128
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http://dx.doi.org/10.1186/s12879-018-3561-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318942PMC
January 2019
13 Reads

Group B streptococcus and legal liability.

Authors:
Oliver Plumb

BJOG 2019 Mar 1;126(4):512. Epub 2019 Jan 1.

Haywards Heath, UK.

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http://dx.doi.org/10.1111/1471-0528.15531DOI Listing
March 2019
1 Read

RNA sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae.

J Dairy Sci 2019 Feb 26;102(2):1761-1767. Epub 2018 Dec 26.

Departamento de Ciências Exatas, Universidade Estadual Paulista-UNESP/FCAV, Jaboticabal 14884-900, Brazil. Electronic address:

The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. Read More

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http://dx.doi.org/10.3168/jds.2018-15516DOI Listing
February 2019
2 Reads

Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip.

Fish Shellfish Immunol 2019 Mar 24;86:999-1008. Epub 2018 Dec 24.

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; State Key Laboratory of Aquaculture Microbiology, College of Fisheries, Huazhong Agricultural University, Wuhan, China. Electronic address:

Streptococcus agalactiae infections are becoming an increasing problem in aquaculture because of significant morbidity and mortality, which restricts the healthy development of tilapia aquaculture. To seek safe and effective prevention measures, a Bacillus subtilis GC5 surface displayed vaccine was prepared and applied orally in tilapia. The study first showed that recombinant spores can engraft in the tilapia intestine. Read More

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http://dx.doi.org/10.1016/j.fsi.2018.12.060DOI Listing
March 2019
2 Reads

Characterization and expression of galectin-3 after Streptococcus agalactiae and Aeromonas hydrophila challenge in GIFT strain Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2019 Mar 21;86:974-980. Epub 2018 Dec 21.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, 430223, China. Electronic address:

In mammals, Galectin-3 has been revealed to be widely expressed in immune cells and played important role in immune reactions. However, Galectin-3 is frequently less reported in teleost. In the present study, a molecular characterization and expression analysis of galectin-3 were conducted in GIFT strain Nile tilapia. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648183084
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http://dx.doi.org/10.1016/j.fsi.2018.12.036DOI Listing
March 2019
13 Reads

Severe aortic regurgitation masked as sepsis-induced ARDS in a patient with endocarditis.

BMJ Case Rep 2018 Dec 4;11(1). Epub 2018 Dec 4.

Internal Medicine, Aventura Hospital and Medical Center, Aventura, Florida, USA.

Septic shock is the most common type of shock in the intensive care unit with an associated mortality close to 50%. Infective endocarditis (IE) is a rare cause of septic shock but carries significant morbidity and mortality. Group B IE (GBS-IE) is an invasive infection with an incidence of approximately 1. Read More

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http://www.bmj.com/lookup/doi/10.1136/bcr-2018-226681
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http://dx.doi.org/10.1136/bcr-2018-226681DOI Listing
December 2018
18 Reads