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Int J Mol Sci 2019 Apr 13;20(8). Epub 2019 Apr 13.

Dipartimento di Biologia e Biotecnologie, Università degli Studi di Pavia, 27100 Pavia, Italy.

Cystic fibrosis (CF) is an autosomal recessive genetic disorder which leads to the secretion of a viscous mucus layer on the respiratory epithelium that facilitates colonization by various bacterial pathogens. The problem of drug resistance has been reported for all the species able to colonize the lung of CF patients, so alternative treatments are urgently needed. In this context, a valid approach is to investigate new natural and synthetic molecules for their ability to counteract alternative pathways, such as virulence regulating quorum sensing (QS). Read More

Intern Med 2019 Apr 17. Epub 2019 Apr 17.

Division of Hematology, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan.

Intern Med 2019 Apr 17. Epub 2019 Apr 17.

Division of General Medicine, Jichi Medical University Saitama Medical Center, Japan.

BMC Genomics 2019 Apr 16;20(1):300. Epub 2019 Apr 16.

Department of Biological Sciences, 6-065 Centennial Centre for Interdisciplinery Science, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada.

Background: Temperate bacteriophages are capable of lysogenic conversion of new bacterial hosts. This phenomenon is often ascribed to "moron" elements that are acquired horizontally and transcribed independently from the rest of the phage genes. Whereas some bacterial species exhibit relatively little prophage-dependent phenotypic changes, other bacterial species such as Stenotrophomonas maltophilia appear to commonly adopt prophage genetic contributions. Read More

BMC Microbiol 2019 Apr 15;19(1):77. Epub 2019 Apr 15.

Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090, Melle, Belgium.

Background: Water quality in the drinking water system (DWS) plays an important role in the general health and performance of broiler chickens. Conditions in the DWS of broilers are ideal for microbial biofilm formation. Since pathogens might reside within these biofilms, they serve as potential source of waterborne transmission of pathogens to livestock and humans. Read More

Plants (Basel) 2019 Apr 4;8(4). Epub 2019 Apr 4.

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.

The main objective of this study was to evaluate the genetic diversity and agricultural significance of bacterial communities associated with the surfaces of selected agronomic plants (carrot, cabbage and turnip). The bacterial diversity of fresh agricultural produce was targeted to identify beneficial plant microflora or opportunistic human pathogens that may be associated with the surfaces of plants. Bacterial strains were screened in vitro for auxin production, biofilm formation and antibiotic resistance. Read More

Int J Mol Sci 2019 Apr 10;20(7). Epub 2019 Apr 10.

Department of Biotechnology and Laboratory Science in Medicine, 11221, National Yang-Ming University, Taipei 11221, Taiwan.

Manganese-dependent superoxide dismutase (MnSOD, SodA) and iron-dependent SOD (FeSOD, SodB) are critical cytosolic enzymes for alleviating superoxide stress. Distinct from the singular gene in most bacteria, harbors two genes, and . The roles of SodA1, SodA2, and SodB of in alleviating superoxide stress were investigated. Read More

J Infect Public Health 2019 Apr 4. Epub 2019 Apr 4.

Saint George Hospital University Medical Center, Faculty of Medicine and Medical Sciences, University of Balamand, P.O. Box 166 378, Achrafieh, Beirut 11 00 2807, Lebanon. Electronic address:

Introduction: Carbapenem resistant organisms (CRO) constitute a large group of bacteria with different mechanisms of resistance and recently increasing global incidence. This rise has ambiguous dynamics and essential local epidemiologic data is lacking.

Materials And Methods: In this retrospective study at the 400-bed Saint George Hospital (SGH) in Beirut, Lebanon, we retrieved electronic laboratory records of all intrinsic and acquired CRO isolates from January 1, 2010 until June 30, 2018. Read More

J Antimicrob Chemother 2019 Mar 3. Epub 2019 Mar 3.

Department of Pharmacy, The Ohio State University Wexner Medical Center, Columbus, OH, USA.

Background: Stenotrophomonas maltophilia is intrinsically resistant to several antibiotics, making it potentially challenging to treat. Studies have demonstrated treatment failures and resistance development with monotherapy (MT); however, clinical data are limited with combination therapy (CT).

Objectives: To compare clinical outcomes with CT versus MT for S. Read More

PLoS One 2019 1;14(4):e0214596. Epub 2019 Apr 1.

No 307 Hospital of PLA of Anhui Medical University, Hefei, China.

Stenotrophomonas maltophilia (S. maltophilia), a multi-drug resistant opportunistic pathogen, is associated with nosocomial and community-acquired infections. Preventive and therapeutic strategies for such infections are greatly needed. Read More

Malays J Med Sci 2018 Mar 27;25(2):40-49. Epub 2018 Apr 27.

Near East University, Department of Clinical Microbiology and Infections Diseases, Nicosia, Cyprus.

Background: is a non-fermentative gram-negative bacillus which is widely recognised as an important nosocomial pathogen causing pneumonia, blood-stream, wound and urinary tract infections, particularly in immunosuppressed patients. The aim of this study was to evaluate a nosocomial outbreak of by in an intensive care unit of a tertiary hospital and evaluate unexpected multiclonality.

Methods: A total of 11 isolates from respiratory cultures in intensive care unit of a 24 bed tertiary hospital obtained over a one months period and one isolate obtained from the nebuliser during environmental screening were investigated. Read More

Prep Biochem Biotechnol 2019 Mar 21:1-8. Epub 2019 Mar 21.

a School of Chemical Engineering and Technology , Tianjin University , Tianjin , PR China.

Polymicrobial biofilms often form on the surfaces of food-processing machinery, causing equipment damage and posing a contamination risk for the foods processed by the system. The composition of the microbial communities that make up these biofilms is largely unknown, especially in the dairy industry. To address this deficit, we investigated the bacterial composition of biofilms that form on the surfaces of equipment during dairy processing using Illumina MiSeq sequencing and culture-dependent methods. Read More

Open Forum Infect Dis 2019 Mar 15;6(Suppl 1):S34-S46. Epub 2019 Mar 15.

JMI Laboratories, North Liberty, Iowa.

Background: () complex and represent frequent causes of hospital-acquired infections. We evaluated the frequency and resistance rates of complex and isolates from medical centers enrolled in the SENTRY Program.

Methods: A total of 13 752 complex and 6467 isolates were forwarded to a monitoring laboratory by 259 participating sites from the Asia-Pacific region, Latin America, Europe, and North America between 1997 and 2016. Read More

Sci Rep 2019 Mar 20;9(1):4917. Epub 2019 Mar 20.

Centro Nacional de Biotecnología, CSIC, 28049, Madrid, Spain.

The emergence of antibiotic resistant Gram-negative bacteria has become a serious global health issue. In this study, we have employed the intrinsically resistant opportunistic pathogen Stenotrophomonas maltophilia as a model to study the mechanisms involved in the acquisition of mutation-driven resistance to antibiotics. To this aim, laboratory experimental evolution studies, followed by whole-genome sequencing, were performed in the presence of the third-generation cephalosporin ceftazidime. Read More

J Neurol Sci 2019 May 14;400:32-33. Epub 2019 Mar 14.

Department of Neurology, University Hospital, Ludwig-Maximilians-University, Munich, Germany.

We describe the first case of a patient with brain abscesses caused by Stenotrophomonas maltophilia as a complication after motor cortex stimulator implantation. Brain abscesses pose a challenge in diagnosis and treatment, because microbiological diagnosis is not always achieved, antibiotic drugs may not penetrate well into the CNS and some bacteria have resistances to typical empirical antibiotic drugs. In this case diagnosis was only made after removal of the stimulator and a long term treatment with antibiotic drugs was necessary. Read More

Turk J Med Sci 2019 04 18;49(2):683-686. Epub 2019 Apr 18.

Background/aim: The aim of this study was to determine the usefulness of tigecycline in combination treatment of Stenotrophomonas maltophilia infections by evaluating the in vitro synergistic effects of tigecycline with various antibiotics using the E-test method.

Materials And Methods: Synergy testing by E-test was performed with various antibiotic combinations in 10 S. maltophilia isolates identified as a cause of infection. Read More

Microb Drug Resist 2019 Apr 13;25(3):317-325. Epub 2019 Mar 13.

4 Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, China.

Development of antibiotic resistance can be achieved either by mutation or by acquiring a resistance gene from foreign sources, with some resistance genes likely originating in microbial populations to counteract antibiotics present in natural ecosystems. In this study, we describe the first report of a strain of nonclinical multidrug-resistant Stenotrophomonas sp. strain G4 with high-level resistance to colistin and meropenem, phylogenetically distinct from well-studied multiple drug-resistant species of Stenotrophomonas maltophilia. Read More

Environ Sci Pollut Res Int 2019 Mar 13. Epub 2019 Mar 13.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, Messina, Italy.

In recent years, the photocatalytic process by using TiO nanoparticles (NPs) has produced a great interest in wastewater treatment due to its interesting features such as low-cost, environmental compatibility, and especially capacity to eliminate persistent organic compounds as well as microorganisms in water. In the present work, the photocatalytic activity of Gd-doped TiO nanopowders, with different doping amount 0.1, 1, and 5 mol% synthesized by the sol-gel method, was studied under UV/Visible irradiation for water treatment application. Read More

Front Microbiol 2019 19;10:221. Epub 2019 Feb 19.

Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome, Italy.

Blood culture (BC) is still the standard for diagnosing bloodstream infections (BSIs), especially those caused by bacteria and fungi. Infection-complicating sepsis or septic shock often occurs at BSI onset, making necessary to improve the diagnostic yield of positive BCs. Among the BC systems currently available, the BACT/ALERT® VIRTUO® (VIRTUO) system has been developed to shorten time to detection (TTD) of positive BCs. Read More

J Pediatr Pharmacol Ther 2019 Jan-Feb;24(1):61-65

Stenotrophomonas maltophilia is an increasingly prevalent cause of nosocomial infections. This report describes a 5-month-old male diagnosed with a S maltophilia ventriculoperitoneal shunt infection after a neurosurgical procedure. Intravenous trimethoprim/sulfamethoxazole and moxifloxacin successfully treated the patient. Read More

Case Rep Oncol 2019 Jan-Apr;12(1):22-28. Epub 2019 Jan 4.

Klinik für Innere Medizin II, Hämatologie und internistische Onkologie, Universitätsklinikum Jena, Jena, Germany.

Infectious complications such as invasive aspergillosis or infection with (SM) in immunocompromised patients are associated with a high mortality rate. Our report concerns a 40-year-old male newly diagnosed very severe aplastic anemia (vSAA) who in consequence of a mosquito bite was suffering from skin lesion and consecutive soft tissue phlegmon subsequent to the administration of antithymocyte globulin; a full-thickness autologous meshed skin graft successfully performed to cover skin ulcera after allogeneic stem cell transplantation (SCT). This unusual case illustrates the importance of appropriate diagnosis, anti-infective therapy and close interdisciplinary diagnostic algorithms to minimalize side effects and the selection of resistant strains and to improve patients' outcome. Read More

Mar Biotechnol (NY) 2019 Apr 14;21(2):276-290. Epub 2019 Feb 14.

Department of Microbiology, Faculty of Pharmacy, University of Granada, 18071, Granada, Spain.

Many Gram-negative aquacultural and agricultural pathogens control virulence factor expression through a quorum-sensing (QS) mechanism involving the production of N-acylhomoserine (AHL) signalling molecules. Thus, the interruption of QS systems by the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), has been proposed as a novel strategy to combat these infections. Given that the symbiotic bacteria of marine invertebrates are considered to be an important source of new bioactive molecules, this study explores the presence of AHL-degrading bacteria among 827 strains previously isolated from the microbiota of anemones and holothurians. Read More

Hemodial Int 2019 Feb 12. Epub 2019 Feb 12.

PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Brunei Darussalam.

We report a case series of seven patients with nonfermentative Gram-negative bacteria infections in a single dialysis center; four patients with Ralstonia pickettii and three patients with Stenotrophomonas maltophilia. Two of the seven patients were admitted to hospital for intravenous antibiotic treatment, while the rest were treated with oral antibiotics at home. Both the admitted patients had temporary vascular catheter infections from the aforementioned pathogens. Read More

Antimicrob Agents Chemother 2019 Apr 27;63(4). Epub 2019 Mar 27.

Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan

is an organism with a remarkable capacity for drug resistance with several antibiotic resistance determinants in its genome. genome codes for L1 and L2, responsible for intrinsic β-lactam resistance. The Smlt3721 gene (denoted ), located downstream of the L2 gene, encodes an inner membrane protein. Read More

Pestic Biochem Physiol 2019 Jan 8;153:161-170. Epub 2018 Dec 8.

Department of Zoology, University College, Thiruvananthapuram, Kerala 695034, India. Electronic address:

Oryctes rhinoceros Linn. is one of the most serious pests of coconuts and other palms. Following bioassay guided method, a larvicidal compound, 22-hydroxyhopane has been isolated for the first time from methanol extract of leaves of Adiantum latifolium Lam. Read More

J Cyst Fibros 2019 Mar 25;18(2):294-298. Epub 2018 Oct 25.

Adult Cystic Fibrosis Centre, Liverpool Heart and Chest Hospital, Liverpool L14 3PE, UK. Electronic address:

Introduction: Stenotrophomonas maltophilia is common in the sputum of people with cystic fibrosis related diabetes (CFRD), raising the question as to whether this is a risk factor for its acquisition. We investigated this at a population level.

Methods: We analysed national Cystic Fibrosis Registry data 2011-2015 for 8047 people with CF > age 6 years, looking at demographics, diagnosis of CFRD, lung function and sputum microbiology; using descriptive and multivariate strategies to establish independent predictors for S. Read More

Klin Lab Diagn 2018;63(9):574-578

Samara State Medical University, 43099, Samara, Russia.

The aim of our research was to evaluate the structure and the determination of the phenotypes of antibiotic resistance of microflora isolated from patients' blood in a multidisciplinary hospital during the period from 2013 to 2017. The material was taken into BacT / ALERT bottles containing nutrient media, followed by incubation of blood. In case of a positive result, the material from the vials was dispersed into dense nutrient media. Read More

Infect Dis (Lond) 2019 May 7;51(5):387-391. Epub 2019 Feb 7.

d Department of Medical Microbiology, Faculty of Medicine , Medical University of Sofia , Sofia , Bulgaria.

Eur J Microbiol Immunol (Bp) 2018 Dec 12;8(4):135-141. Epub 2018 Dec 12.

Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany.

Background: The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials.

Methods: Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Read More

Drugs 2019 Feb;79(3):271-289

Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.

Cefiderocol is an injectable siderophore cephalosporin discovered and being developed by Shionogi & Co., Ltd., Japan. Read More

Clin Microbiol Infect 2019 Jan 29. Epub 2019 Jan 29.

Division of Infectious Diseases, Microbiology and Preventive Medicine, Hospital Universitario Virgen Macarena / Department of Medicine, University of Seville / Biomedicine Institute of Seville (IBiS), Seville, Spain.

Scope: The aim of these guidelines is to provide recommendations for decolonizing regimens targeting multidrug-resistant Gram-negative bacteria (MDR-GNB) carriers in all settings.

Methods: These evidence-based guidelines were produced after a systematic review of published studies on decolonization interventions targeting the following MDR-GNB: third-generation cephalosporin-resistant Enterobacteriaceae (3GCephRE), carbapenem-resistant Enterobacteriaceae (CRE), aminoglycoside-resistant Enterobacteriaceae (AGRE), fluoroquinolone-resistant Enterobacteriaceae (FQRE), extremely drug-resistant Pseudomonas aeruginosa (XDRPA), carbapenem-resistant Acinetobacter baumannii (CRAB), cotrimoxazole-resistant Stenotrophomonas maltophilia (CRSM), colistin-resistant Gram-negative organisms (CoRGNB), and pan-drug-resistant Gram-negative organisms (PDRGNB). The recommendations are grouped by MDR-GNB species. Read More

Chest 2019 Jan 24. Epub 2019 Jan 24.

Washington Hospital Center, Washington, DC.

Background: Carbapenem resistance is a growing concern. Applying a novel algorithm, we examined epidemiology and outcomes of carbapenem resistance among gram-negative pathogens in hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP).

Methods: In a retrospective cohort design within the Premier Research database (2009-2016), all hospitalized adult patients with a gram-negative organism in a respiratory or blood culture specimen who fit criteria for HAP/VAP based on International Classification of Diseases, Ninth Revision, Clinical Modification, codes were included in the study. Read More

Int J Infect Dis 2019 Apr 23;81:1-3. Epub 2019 Jan 23.

Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, China; Department of Pathology and Laboratory Medicine, UCLA School of Medicine, Los Angeles, CA, USA. Electronic address:

We report a rare case of culture negative L4-L5 discitis and epidural abscess in an immunocompetent child who had dry cupping therapy performed to treat low back strain. The causative pathogen was identified as Stenotrophomonas maltophilia by shotgun metagenomic sequencing of spinal cord aspirate after more than one month of unsuccessful empirical treatment with 6 different antibiotics. The patient was successfully treated with Sulfamethoxazole-trimethoprim and minocycline. Read More

Mikrobiyol Bul 2019 Jan;53(1):1-11

İnönü University Faculty of Medicine, Department of Biostatistics and Medical Informationt, Malatya, Turkey.

Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) is a new method that is increasingly used in microbiology laboratories due to its ability of reliable and rapid identification (ID) of bacteria and fungi. However, some problems emerge in the routine clinical diagnosis since only one gram-negative selective medium has been suggested up to date. Though EMB agar is one of the traditional gram-negative selective media, there is no data in the scientific literature, about the ID performance of MALDI TOF MS with the gram-negative bacteria grown on this medium. Read More

Plant Dis 2018 Mar 24;102(3):628-639. Epub 2018 Jan 24.

Jiangsu Provincial Anfeng Biogenic Pesticide Engineering Center Co., Ltd.; Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University; Jiangsu Provincial Biogenic Pesticide Engineering Center; and Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University.

To identify new bacterial antagonists for cucurbit downy mildew (CDM) caused by Pseudoperonospora cubensis, 163 bacterial isolates were recovered from different microenvironments of field-grown cucumber plants. In the greenhouse, 19 representative isolates were applied to cucumber plants as a foliar spray (FS); 7 isolates achieved the efficacy over 60% against CDM, with 5 (DS22, HS10, DP14, HP4, and DS57) identified as Bacillus pumilus, B. licheniformis, Enterobacter sp. Read More

Biofouling 2018 Nov 21;34(10):1150-1160. Epub 2019 Jan 21.

a LEPABE, Department of Chemical Engineering, Faculty of Engineering , University of Porto , Porto , Portugal.

In this study, 20 heterotrophic bacteria from a minimally processed vegetables (MPV) plant were tested for their susceptibilities to five antibiotics (tetracycline, erythromycin, ampicillin, levofloxacin and ciprofloxacin), their (co)aggregation abilities and their survival under gastric simulated conditions. Peracetic acid (PA) and sodium hypochlorite (SH), both at 50 ppm, were evaluated for their abilities to control biofilms of these bacteria. In general, the Gram-negative bacteria were found to be more resistant to the selected antibiotics. Read More

J Bacteriol 2019 Apr 13;201(7). Epub 2019 Mar 13.

Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA

The airway fluids of cystic fibrosis (CF) patients contain local pH gradients and are more acidic than those of healthy individuals. pH is a critical factor that is often overlooked in studies seeking to recapitulate the infection microenvironment. We sought to determine the impact of pH on the physiology of a ubiqituous yet understudied microbe, Phylogenomics was first used to reconstruct evolutionary relationships between 74 strains of (59 from CF patients). Read More

Can J Microbiol 2019 Apr 11;65(4):308-321. Epub 2019 Jan 11.

c Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia 11566, Cairo, Egypt.

An important mechanism for microbial resistance to mercury is its reduction into elemental mercury (facilitated by the merA gene). Thirty-eight microbial isolates from a variety of wastewater sources in Egypt were collected. Approximately 14 of the 38 isolates exhibited not only a high degree of tolerance to mercury (up to 160 ppm) but also a high degree of resistance to other tested heavy metals (Cu, Co, Ni, and Zn). Read More

PeerJ 2019 3;7:e6102. Epub 2019 Jan 3.

Instituto de Biociencias, Universidad Autónoma de Chiapas, Tapachula, Chiapas, Mexico.

Chitin is the second most abundant organic compound in nature and represents a rich carbon and nitrogen source that is primarily transformed by bacterial communities. Bacteria capable of gradually hydrolyzing chitin into N-acetylglucosamine monomers can have applications in the transformation of residues from shrimp and other crustaceans. The objective of the present study was to isolate, characterize and identify microorganisms with high chitinolytic activity. Read More

PLoS One 2019 8;14(1):e0210201. Epub 2019 Jan 8.

UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France.

Pseudomonas aeruginosa is the main cause of chronic airway infection in cystic fibrosis (CF). However, for unclear reasons some patients are never colonized by P. aeruginosa. Read More

Front Microbiol 2018 19;9:3178. Epub 2018 Dec 19.

Environmental Microbiology and Microbial Biotechnology Laboratory, Department of Biotechnology, University of Verona, Verona, Italy.

SeITE02 and sp. MPV1 were isolated from the rhizosphere soil of the selenium-hyperaccumulator legume and waste material from a dumping site for roasted pyrites, respectively. Here, these bacterial strains were studied as cell factories to generate selenium-nanostructures (SeNS) under metabolically controlled growth conditions. Read More

Lupus 2019 Feb 7;28(2):249-252. Epub 2019 Jan 7.

1 Pediatric Rheumatology Unit, Children's Institute, Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.

Childhood-onset systemic lupus erythematosus (cSLE) is a chronic inflammatory multisystem autoimmune disease that requires multiple differential diagnoses. Munchausen by proxy syndrome (MBPS) is a form of child abuse, where a caregiver intentionally creates a medical history and induces or fabricates signs or disease in a patient. To our knowledge, there is no case report of MBPS mimicking cSLE diagnosis. Read More

J Vet Res 2018 Sep 10;62(3):261-267. Epub 2018 Dec 10.

Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland.

Changes occurring in freshwater ecosystems seem to be fundamental in the development of all microorganisms, including those pathogenic to fish. This has been especially evident in recent years during which dynamic variations in bacterial fish pathology have been observed. Gram-negative bacteria commonly known to be pathogenic to fish, like spp. Read More

Front Microbiol 2018 6;9:2872. Epub 2018 Dec 6.

Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.

The massive and irrational use of antibiotics in livestock productions has fostered the occurrence and spread of resistance to "old class antimicrobials." To cope with that phenomenon, some regulations have been already enforced in the member states of the European Union. However, a role of livestock animals in the relatively recent alerts on the rapid worldwide increase of resistance to last-choice antimicrobials as carbapenems is very unlikely. Read More

Enzyme Microb Technol 2019 Feb 10;121:37-44. Epub 2018 Nov 10.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Escherichia coli is one kind of the simple and excellent biosystem to overexpress heterologous enzymes, such as keratinolytic protease, an excellent enzyme to hydrolyze keratin substrate for broad industrial application. However, protein expression in E. coli frequently faces some problems such as inactive and inclusion body formation. Read More

Ecotoxicol Environ Saf 2019 Apr 12;170:418-426. Epub 2018 Dec 12.

College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Hai Dian District, Beijing 100193, China. Electronic address:

Vermicomposting of livestock manure using housefly larvae is a promising biotechnology for waste reduction and control of antibiotic pollution. Monensin (MON), an ionophore polyether antibiotic (IPA), is widely used in broiler feed to control coccidiosis. However, MON residues in litter have become a major source of pollution in the environment. Read More

Front Microbiol 2018 27;9:2850. Epub 2018 Nov 27.

EA 7380 Dynamyc, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est Créteil, Créteil, France.

(Af) and (Sm) are pathogenic microorganisms, which coexist in the respiratory tract of cystic fibrosis (CF) patients. We recently developed an model of mixed biofilm associating Af ATCC 13073-GFP (Af13073) and Sm ATCC 13637 (Sm13637) and described an antibiosis effect. The present study aim was to assess the antibiosis of Sm on Af using different strains and to analyze the potential synergistic virulence of these strains in an model. Read More

Front Microbiol 2018 22;9:2871. Epub 2018 Nov 22.

Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Barcelona, Spain.

The polymyxin antibiotic colistin shows activity against . However, an increased incidence of colistin-resistant isolates has been recently observed. In addition, evaluation of colistin susceptibility for this organism has been problematic. Read More

Mikrobiyol Bul 2018 Oct;52(4):329-339

Haydarpaşa Numune Training and Research Hospital, Department of Medical Microbiology, Istanbul, Turkey.

Empiric treatment with broad-spectrum antibiotics exerts condensed pressure in intensive care units (ICUs) for the selection of multidrug-resistant bacteria. Multidrug-resistant gram-negative bacteria became the focus of interest owing to limited treatment options and negative effects on patient survival. Cumulative antibiograms can guide selection of correct empiric treatment, de-escalation treatment according to antibiogram results and development of policies in fight against antibiotic resistance. Read More

Microbiol Resour Announc 2018 Aug 9;7(5). Epub 2018 Aug 9.

Curtin Water Quality Research Centre, Curtin University, Perth, Western Australia, Australia.

We report here the complete genome sequence of Stenotrophomonas maltophilia AB550, a multidrug- and solar radiation-resistant strain isolated from the effluents of an urban wastewater treatment plant in Western Australia. The genome consists of a single 4.9-Mb chromosome. Read More