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Front Microbiol 2018 21;9:2809. Epub 2018 Nov 21.

Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.

Colistin is one of the last resort antimicrobials for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. After the emergence of transferable colistin resistance genes (-1-5), a reliable culture-based screening method to detect colonization with colistin-resistant Gram-negative bacteria (CRGN) is needed. The objective of this study was to test the performance of SuperPolymyxin™ medium to screen for CRGN in stool samples and to compare different methods for the confirmation of colistin resistance (e. Read More

Front Cell Infect Microbiol 2018 12;8:401. Epub 2018 Nov 12.

Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia.

is a multi-drug-resistant global opportunistic nosocomial pathogen, which possesses a huge number of virulence factors and antibiotics resistance characteristics. Iron has a crucial contribution toward growth and development, cell growth and proliferation, and pathogenicity. The bacterium found to acquire iron for its cellular process through the expression of two iron acquisition systems. Read More

Zhonghua Shao Shang Za Zhi 2018 Nov;34(11):802-808

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China.

To analyze the distribution and drug resistance of pathogens from the wounds of thermal burn patients, so as to provide reliable basis for the rational use of antibiotics and the effective control over nosocomial infection. Wound samples of 1 310 thermal burn patients admitted into our burn wards from January 2012 to December 2017 were collected and retrospectively analyzed. API bacteria identification panels and automatical bacteria identification equipment were used to identify pathogens. Read More

Plant Mol Biol 2018 Dec;98(6):579

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.

This article (Zhou et al. 2018) has been retracted by the authors because the sequence BIBAC 002A111F06 was incorrectly assigned to the wrong bacterial species. The BIBAC 002A111F06 sequence (GenBank Accession KC129717) reported in the paper was attributed to Populus euphratica Oliv. Read More

J Arthroplasty 2018 Oct 25. Epub 2018 Oct 25.

Department of Molecular Pathology, National Research Institute of Tuberculosis and Lung Diseases, Shahid Behshti University of Medical Sciences, Tehran, Iran; Department of Molecular Biology, Dr. Khosroshahi's Pathobiology Laboratory, Tehran, Iran.

Background: PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) techniques have been used for the diagnosis of bacteria in some infections. In this study, we aimed to evaluate the diagnostic accuracy of PCR for the diagnosis of prosthetic joint infections (PJI) and to identify isolated microorganisms, using the RFLP method.

Methods: During January 2015 to January 2018, patients who were suspected of having PJI after arthroplasty surgery or were candidates for revision surgery due to loosening of implant entered the study. Read More

Int J Antimicrob Agents 2018 Nov 21. Epub 2018 Nov 21.

International Health Management Associates, Inc., Schaumburg, Illinois, USA.

Cefiderocol (S-649266) is a parenteral siderophore cephalosporin currently in Phase III of clinical development. In this study, we determined the in vitro susceptibility of a 2015-2016 collection of 8,954 clinical isolates of Gram-negative bacilli (GNB), provided by 100 clinical laboratories in North America and Europe, to cefiderocol and comparators using the Clinical and Laboratory Standards Institute broth microdilution method. Iron-depleted cation-adjusted Mueller-Hinton broth was used to test cefiderocol. Read More

Antonie Van Leeuwenhoek 2018 Nov 22. Epub 2018 Nov 22.

Laboratory of Biotechnology, Chulabhorn Research Institute, 54 Kamphaeng Phet 6 Road, Talat Bang Khen, Lak Si, Bangkok, 10210, Thailand.

Inactivation of ahpC, encoding alkyl hydroperoxide reductase, rendered Stenotrophomonas maltophilia more resistant to HO; the phenotype was directly correlated with enhanced total catalase activity, resulting from an increased level of KatA catalase. Plasmid-borne expression of ahpC from pAhpC could complement all of the mutant phenotypes. Mutagenesis of the proposed AhpC peroxidactic and resolving cysteine residues to alanine (C47A and C166A) on the pAhpC plasmid diminished its ability to complement the ahpC mutant phenotypes, suggesting that the mutagenized ahpC was non-functional. Read More

J Stomatol Oral Maxillofac Surg 2018 Nov 19. Epub 2018 Nov 19.

Recep Tayyip Erdogan University, Department of Pathology, Rize, Turkey.

An 81-year-old woman with diabetes mellitus presented with signs of orbital cellulitis and a gangrenous ulcer on the right palate. Biopsy analysis confirmed a diagnosis of necrotizing fasciitis. Surgical debridement of necrotic tissues and enucleation of the right eye were performed. Read More

Infect Drug Resist 2018 2;11:2047-2061. Epub 2018 Nov 2.

Department for Internal Medicine I/Gastroenterology and Hepatology, University Hospital Frankfurt, Frankfurt am Main, Germany,

Background And Aims: Spontaneous bacterial peritonitis (SBP) is a severe complication of decompensated cirrhosis. The prevalence of multidrug-resistant organisms (MDROs) in patients with cirrhosis is increasing. Identification of patients at risk for SBP due to MDROs (ie, SBP with the evidence of MDROs or in ascitic culture, MDRO-SBP) is crucial to the early adaptation of antibiotic treatment in such patients. Read More

Clin Microbiol Infect 2018 Nov 15. Epub 2018 Nov 15.

Division of Infectious Diseases, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.

Background: Fluoroquinolones (FQs) are a popular alternative to trimethoprim-sulfamethoxazole (STX) for Stenotrophomonas maltophilia infections.

Objectives: To compare the effects of FQs and STX on mortality of S. maltophilia infections DATA SOURCES: PubMed and EMBASE STUDY ELIGIBILITY CRITERIA: Clinical studies reporting mortality outcomes of S. Read More

Ecotoxicol Environ Saf 2018 Nov 13;169:161-168. Epub 2018 Nov 13.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; National Engineering Laboratory for VOCs Pollution Control Material & Technology, University of Chinese Academy of Sciences, Beijing 101408, PR China. Electronic address:

The characteristics of bioaerosol emissions from wastewater treatment plants (WWTPs) have attracted extensive attention. The anaerobic-anoxic-oxic (AO) process, which uses the activated sludge approach to wastewater treatment, is the most widely used process in WWTPs. Concentration, size distribution, population, and exposure risk from bacteria and fungi in bioaerosols of WWTPs using the AO process were studied in this work. Read More

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2018 Oct;30(10):933-938

Department of ICU, Shexian People's Hospital, Huangshan 245200, Anhui, China (Hu ZC); Department of ICU, First Affiliated Hospital of China University of Science and Technology, Hefei 230001, Anhui, China (Zhou SS). Corresponding author: Zhou Shusheng, Email:

Objective: To investigate the risk factors of ventilator-associated pneumonia (VAP) and the distribution and drug resistance of pathogens in intensive care unit (ICU) of county hospital.

Methods: 234 patients on mechanical ventilation for more than 48 hours admitted to ICU of Shexian People's Hospital of Huangshan City from January 2016 to June 2018 were enrolled. The clinical data of all patients including gender, age, past medical history, exposure to antibiotics, medication, the duration of mechanical ventilation, the length of ICU stay, serum albumin, tracheotomy, re-intubation, prognosis, and pathogenic bacteria and drug sensitivity test of VAP patients were collected. Read More

Eur J Clin Microbiol Infect Dis 2018 Nov 13. Epub 2018 Nov 13.

Department of emergency medicine, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The World Health Organization (WHO) proposed a global priority pathogen list (PPL) of multidrug-resistant (MDR) bacteria. Our current objective was to provide global expert ranking of the most serious MDR bacteria present at intensive care units (ICU) that have become a threat in clinical practice. A proposal addressing a PPL for ICU, arising from the WHO Global PPL, was developed. Read More

Data Brief 2018 Dec 23;21:966-971. Epub 2018 Oct 23.

The Devision of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, PR China.

Herein, an algicidal strain, GA1, was isolated from a marine dinoflagellate and its genome was sequenced using next-generation sequencing technology. The genome size of GA1 was determined to be 5.92 Mb with a G+C content of 62. Read More

Clin Infect Dis 2018 Nov;67(suppl_2):S174-S178

Department of Hematology, Shanghai Jiao Tong University-affiliated Shanghai General Hospital, China.

Background: Bacterial infections are very common among patients with hematological diseases. Scant data are available regarding differences in the epidemiology and biological features of bacterial infections in neutropenic and non-neutropenic patients.

Methods: The aim of this survey was to compare the bacterial pathogens in neutropenic and non-neutropenic patients in the same ward during an 8-year period. Read More

Infect Dis (Lond) 2018 Nov 13:1-11. Epub 2018 Nov 13.

b Department of Medical Microbiology , Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.

Stenotrophomonas maltophilia is an increasingly prevalent opportunistic pathogen responsible for a wide range of nosocomial infections in intensive care unit patients, life-threatening diseases in immunocompromised haematology-oncology patients and chronic pulmonary infections in individuals with cystic fibrosis. Therapy of these infections is problematic due to the remarkable intrinsic antimicrobial resistance of the species and to acquired resistance to multiple antimicrobial agents. As this organism is a low-grade pathogen, the pathogenesis of S. Read More

Eur J Clin Microbiol Infect Dis 2018 Nov 12. Epub 2018 Nov 12.

Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul, 06351, South Korea.

Hemorrhagic pneumonia (HP) is known as the clinical manifestation of Stenotrophomonas maltophilia infection, while catheter-related blood stream infection (CRBSI) is the common clinical presentation of S. maltophilia bacteremia (SMB). The purpose of this study is to evaluate the risk factors for mortality in hematologic malignancy patients with SMB and to analyze clinical and microbiological characteristics of HP associated with SMB and CRBSI. Read More

Br J Ophthalmol 2018 Nov 12. Epub 2018 Nov 12.

Advanced Eye Center, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

Purpose: To study features, management and outcomes of cluster endophthalmitis following intravitreal bevacizumab (BCZ) injection in North India.

Methods: In this retrospective study, 28 patients (23 men) (mean age of 59.07±13 years) who received intravitreal injection of BCZ were included. Read More

Ophthalmic Plast Reconstr Surg 2018 Nov/Dec;34(6):602-603

Int J Antimicrob Agents 2018 Nov 8. Epub 2018 Nov 8.

Texas Tech University Health Sciences Center, Department of Pharmacy Practice, 5920 Forest Park Rd, Suite 400, Dallas, TX 75235, USA; VA North Texas Health Care System, 4500 S Lancaster, Dallas, TX 75216, USA; University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 7539, USA. Electronic address:

Piperacillin/tazobactam has been associated with nephrotoxicity in patients receiving vancomycin. Its impact on nephrotoxicity in patients with gram-negative bacteremia (GNB) is unclear. This study aims to evaluate piperacillin/tazobactam's impact on nephrotoxicity in patients with GNB. Read More

Microb Drug Resist 2018 Nov 7. Epub 2018 Nov 7.

Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP) , Ribeirão Preto, Brazil .

Stenotrophomonas maltophilia is an opportunist pathogen that has intrinsic resistance to the majority of antibiotics and has a high ability to adapt in different environments; however, there are few reports of acquired resistance genes in S. maltophilia. The aim of this study was to investigate the antimicrobial resistance profile, the presence of mutations in the quinolone-resistance determining region, the presence of acquired resistance genes, and the different plasmid families in S. Read More

Appl Environ Microbiol 2018 Oct 26. Epub 2018 Oct 26.

Department of Clinical Microbiology. IIS-Fundación Jiménez Díaz, UAM. Av. Reyes Católicos, 2.28040 Madrid, Spain.

Joint prosthesis failure is mainly related to aseptic loosening and prosthetic joint infections, both of which are associated with high morbidity and substantial costs for patients and health systems. The development of a biomaterial that is capable of stimulating bone growth while minimizing bacterial adhesion would reduce the incidence of prosthetic failure. We report antibacterial and osteostimulatory effects in a novel F-P-doped TiO oxide film grown on Ti-6Al-4V alloy with a nanostructure of bottle-shape nanotubes -bNT samples-using five bacterial species (, , , , and ) and MCT3T3-E1 osteoblastic cells. Read More

Data Brief 2018 Dec 4;21:263-268. Epub 2018 Oct 4.

Department of Clinical Microbiology, Christian Medical College, Vellore, Tamilnadu, India.

is an important emerging nosocomial pathogen with broad level multi-drug resistance. There is a lack of genomic information on to understand the antimicrobial resistance (AMR) mechanism behind. The data article reports on whole genome sequence information of 9 clinical strains isolated from a tertiary care hospital in India. Read More

Microbiologyopen 2018 Oct 24:e745. Epub 2018 Oct 24.

Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.

The objective of this study was to investigate the impact of a high-grain (HG) diet on the microbial fermentation, the composition of the mucosa-associated bacterial microbiota, and the gene expression of tight junction proteins in the small intestine of goats. In the present study, we randomly assigned 10 male goats to either a hay diet (n = 5) or a HG diet (56.5% grain; n = 5) and then examined changes in the bacterial community using Illumina MiSeq sequencing and the expression of tight junction proteins using qRT-PCR in the mucosa of the small intestine. Read More

J Antimicrob Chemother 2018 Oct 23. Epub 2018 Oct 23.

Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.

Objectives: We investigated the in vitro activities of cefiderocol, ceftazidime/avibactam, ceftolozane/tazobactam and other related drugs against imipenem-resistant Pseudomonas aeruginosa, imipenem-resistant Acinetobacter baumannii and Stenotrophomonas maltophilia isolates.

Methods: Non-duplicated bacteraemia isolates (n = 300) of imipenem-resistant P. aeruginosa (n = 100), imipenem-resistant A. Read More

Med Mal Infect 2018 Dec 15;48(8):543-546. Epub 2018 Oct 15.

Servicio de Enfermedades Infecciosas, Hospital Ramón y Cajal, Madrid, España.

Respiration 2018 Oct 17:1-10. Epub 2018 Oct 17.

Kliniken für Pneumologie und Infektiologie, Thoraxzentrum Ruhrgebiet, Herne, Germany.

Background: The impact of respiratory tract pathogens and infection on outcomes in patients with prolonged weaning is largely unknown.

Objective: We studied predictors of weaning outcomes (death and failure to achieve spontaneous ventilation) in a population treated during a 3.5-year period in a specialized and certified weaning centre. Read More

Antimicrob Agents Chemother 2018 Oct 15. Epub 2018 Oct 15.

Department of Medicine, Case Western Reserve University, Cleveland, OH

Activity of the siderophore cephalosporin cefiderocol, targeted for activity against carbapenem resistant Gram-negatives, was determined by microdilution in iron-depleted Mueller Hinton broth against characterized carbapenem resistant complex, , and MICs against , and were 1, 0.25 and 0.5 mg/L, respectively. Read More

Environ Pollut 2019 Jan 6;244:66-71. Epub 2018 Oct 6.

School of Marine Science and Technology, Tianjin University, Tianjin, 300072, China; School of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, China. Electronic address:

This paper investigated 10 carbapenemase genes and selected the hosts of these genes in the estuary of Bohai Bay. The results showed that the OXA-58 producer accounted for a large percentage of carbapenem resistant bacteria in the sampling points, whereas the VIM, KPC, NDM, IMP, GES, OXA-23, OXA-24, OXA-48 and OXA-51 producers were not detected in the study. In addition, 9 bacterial genera with 100% identical bla sequences, including Pseudomonas, Rheinheimera, Stenotrophomonas, Shewanella, Raoultella, Vibrio, Pseudoalteromonas, Algoriphagus, Bowmanella and Thalassospira, were isolated from seawater. Read More

Int J Food Microbiol 2018 Oct 2;290:86-95. Epub 2018 Oct 2.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling 712100, China; National Engineering Research Center of Agriculture Integration Test (Yangling), Yangling 712100, China. Electronic address:

In this work, psychrotrophs known to cause food spoilage were isolated from commercial food products. Further, temperature sensitivities and volatile organic compounds of the representative strains were characterized to evaluate the population heterogeneity. A total of 490 isolates belonging to 38 genera of 20 families were identified from 30 psychrotroph-positive samples, among which Gram-negative bacteria occurred frequently. Read More

Bioresour Technol 2018 Oct 6;272:105-113. Epub 2018 Oct 6.

Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400044, China; Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400044, China.

Mustard tuber wastewater (MTWW) was used as both anolyte and catholyte in biocathode microbial fuel cell (BMFC). The results showed simultaneous nutrient and carbon removal and electricity generation were realized in BMFC. Excellent Chemical Oxygen Demand (COD) removal occurred in both anode (>90%) and cathode (>91%). Read More

Chemosphere 2019 Jan 6;215:142-152. Epub 2018 Oct 6.

China Machinery TDI International Engineering Co., Ltd., Beijing, 100083, China.

In this study, characteristics of dissolved organic matter (DOM) and bacterial community structure in rice washing drainage (RWD)-based groundwater denitrification systems inoculated with and without seeding sludge were investigated. Complete nitrate removal was achieved with a maximum denitrification rate of 64.1 mg NO-N·(gVSS·h). Read More

3 Biotech 2018 Oct 3;8(10):437. Epub 2018 Oct 3.

1Department of Civil Engineering, Regional Campus Anna University, Tirunelveli, 627007 India.

Single chamber air cathode microbial fuel cell (MFC) is a promising and sustainable technology to generate electricity. In the present study, the potential of air cathode MFC treating dye processing wastewater was investigated at various organic loads with interest focused on power densities, organic removal and coulombic efficiencies. The highest power density of about 515 mW/m (6. Read More

An Acad Bras Cienc 2018 Jul-Sep;90(3):2741-2752

Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.

Accidents caused by leaks and/or spills on soils need to be addressed. Natural attenuation, biostimulation and bioaugmentation can be useful bioremediation strategies for decontamination processes in soils of diesel/biodiesel mixtures. The purpose of this study was to evaluate the degradation rate of the different fuels (B0, B20 and B100) in an ultisol under natural attenuation and biostimulation/bioaugmentation during 60 days of incubation in a controlled microcosm simulating a surface spill over soil. Read More

Food Microbiol 2019 Feb 7;77:10-20. Epub 2018 Aug 7.

Microbiome Core Facility, Department of Cell Biology and Physiology, School of Medicine, University of North Carolina, Chapel Hill, NC, 27599, USA.

Limited documentation of the cucumber fermentation microbiome has impeded the understanding of the role of microbes on the quality of finished products. We characterized the microbiome of fresh and fermented cucumber samples using culture dependent and independent techniques, with an emphasis on the non-lactic acid bacteria (non-LAB) population. Insubstantial microbiome variations were observed among fresh cucumber types with Rhizobium (31. Read More

Sci Total Environ 2019 Feb 2;650(Pt 2):2355-2363. Epub 2018 Oct 2.

Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of Ministry of Education, Shaanxi Key Laboratory of Land Consolidation, School of Environmental Science and Engineering, Chang'an University, Xi'an 710054, PR China.

The co-occurrence of heavy metal contamination of soils and increasing air temperature can affect the microbial community in rhizosphere soils by altering the allocation of plant photosynthates to roots. Here, we investigated the community structure of bacteria, fungi, ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA) in the rhizosphere of Robinia pseudoacacia L. seedlings exposed to elevated air temperature (+1. Read More

J Agric Food Chem 2018 Oct 11;66(42):11064-11072. Epub 2018 Oct 11.

Department of Biotechnology, College of Life Sciences , Ritsumeikan University , Kusatsu , Shiga 525-8577 , Japan.

Stenotrophomonas maltophilia HS1 exhibits l-amino acid ester hydrolase (SmAEH) activity, which can synthesize dipeptides such as Ile-Trp, Val-Gly, and Trp-His from the corresponding amino acid methyl esters and amino acids. The gene encoding SmAEH was cloned and expressed in Escherichia coli and was purified and characterized. SmAEH shared 77% sequence identity with a known amino acid ester hydrolase (AEH) from Xanthomonas citri, which belongs to a class of β-lactam antibiotic acylases. Read More

PLoS One 2018 1;13(10):e0203941. Epub 2018 Oct 1.

Department of Medical Biotechnologies, University of Siena, Siena, Italy.

Stenotrophomonas maltophilia and Burkholderia cepacia complex (Bcc) have been increasingly recognized as relevant pathogens in hospitalized, immunocompromised and cystic fibrosis (CF) patients. As a result of complex mechanisms, including biofilm formation and multidrug resistance phenotype, S. maltophilia and Bcc respiratory infections are often refractory to therapy, and have been associated with a worse outcome in CF patients. Read More

Infect Drug Resist 2018 18;11:1511-1519. Epub 2018 Sep 18.

Department of Microbiology, Immunology, and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa,

Background: The spectrum of pathogens causing bloodstream infections (BSIs) in cancer patients has shown significant fluctuations in different geographical areas and time. We studied the microbial spectrum and drug-resistance profile of pathogens causing BSIs in febrile cancer patients at a referral hospital in Ethiopia.

Methods: This cross-sectional study was conducted between December 2011 and June 2012 at Tikur Anbessa Hospital in Addis Ababa. Read More

Environ Pollut 2018 Dec 22;243(Pt B):1206-1216. Epub 2018 Sep 22.

Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Activated sludge (AS) has been regarded as the main driver in the removal of organic pollutants such as pesticides due to a high diversity and abundance of microorganisms. However, little is known about the biodegradation genes (BDGs) and pesticide degradation genes (PDGs) harbored in the AS from wastewater treatment plants (WWTPs). In this study, we explored the bacterial communities and BDGs/PDGs in the AS from five WWTPs affiliated with pesticide factories across four consecutive seasons based on high-throughput sequencing. Read More

ChemSusChem 2018 Sep 28. Epub 2018 Sep 28.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ, Delft, The Netherlands.

Epimerization of cholic and chenodeoxycholic acid (CA and CDCA, respectively) is a notable conversion for the production of ursodeoxycholic acid (UDCA). Two enantiocomplementary hydroxysteroid dehydrogenases (7α- and 7β-HSDHs) can carry out this transformation fully selectively by specific oxidation of the 7α-OH group of the substrate and subsequent reduction of the keto intermediate to the final product (7β-OH). With a view to developing robust and active biocatalysts, novel NADH-active 7β-HSDH species are necessary to enable a solely NAD -dependent redox-neutral cascade for UDCA production. Read More

Int J Antimicrob Agents 2018 Sep 18. Epub 2018 Sep 18.

Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Non-fermenting Gram negative bacteria are increasingly being cultured in respiratory samples from people with cystic fibrosis (CF). This study aimed to determine the susceptibility of clinical CF respiratory isolates from distinct geographical regions to a range of antimicrobials. A total of 286 isolates (106 Stenotrophomonas maltophilia, 100 Burkholderia spp. Read More

Ann Med Surg (Lond) 2018 Oct 6;34:62-65. Epub 2018 Sep 6.

Hospital SS. Spirito e Vito, Alcamo, ASP Trapani, Sicily, Italy.

Introduction: Stenotrophomonas Malthophilia (SM) is generally considered a nosocomial pathogen but it has also been reported as a cause of community-acquired systemic infection. We reported a rare case of SM multi-organ infection involving the liver and the left ocular region.

Presentation Of The Case: A 64 years old man presented with fever for 4 days and acute blindness of the left eye. Read More

Int J Environ Health Res 2018 Sep 19:1-10. Epub 2018 Sep 19.

d Faculty of Agriculture , University of Zagreb , Zagreb , Croatia.

The carbapenem-resistant bacteria (CRB) are currently at the top of the WHO priority list of bacteria that pose the greatest threat to human health. Considering that soil is one of the important environments for the emergence of antibiotic-resistant bacteria, we isolated and quantified cultivable CRB in soils across Croatia, including ones affected by illegal dumps. We cultivated CRB at two temperatures, distinguishing between the intrinsically resistant CRB (37°C, mostly Stenotrophomonas spp. Read More

Chemosphere 2018 Dec 1;213:215-225. Epub 2018 Sep 1.

Department of Microbiology, Cornell University, Ithaca, NY, 14853, USA. Electronic address:

Triclosan, a broad-spectrum antimicrobial, has been widely used in pharmaceutical and personal care products. It undergoes limited degradation during wastewater treatment and is present in biosolids, most of which are land applied in the United States. This study assessed the impact of triclosan (0-100 mg kg) with and without biochar on soil bacterial communities. Read More

BMC Public Health 2018 Sep 15;18(1):1121. Epub 2018 Sep 15.

Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Background: Data on the pathogens responsible for nosocomial bloodstream infection (BSI) and their antimicrobial resistance (AMR) in Hubei province are limited. This study was conducted to determine the major pathogens causing BSI and to characterize their AMR.

Methods: Data from the China Antimicrobial Resistance Surveillance System (CARSS) from 2014 to 2016 were analyzed retrospectively. Read More

Anal Chem 2018 Oct 27;90(20):12123-12130. Epub 2018 Sep 27.

Department of Chemistry and Biochemistry , Queens College-City University of New York , Flushing , New York 11367 , United States.

Fast and cost-efficient detection and identification of bacteria in food and water samples and biological fluids is an important challenge in bioanalytical chemistry. It was shown recently that bacteria can be detected by measuring the decrease in the diffusion current to the ultramicroelectrode caused by cell collisions with its surface. To add selectivity to the bacteria detection, herein we show the possibility of collision experiments with the signal produced by electrochemical activity of bacterial cells reducing (or oxidizing) redox species. Read More

Microb Ecol 2018 Sep 12. Epub 2018 Sep 12.

Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India.

Genetic and functional diversity of osmotolerant bacterial endophytes colonizing the root, stem, and leaf tissues of pearl millet genotypes differing in their drought susceptibility was assessed. Two genotypes of pearl millet, viz., the drought tolerant genotype TT-1 and the drought susceptible genotype PPMI-69, were used in the present study. Read More

Toxins (Basel) 2018 Sep 8;10(9). Epub 2018 Sep 8.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China.

Microcystin-LR (MC-LR) is the most toxic and frequently detected monocyclic heptapeptide hepatotoxin produced by cyanobacteria, which poses a great threat to the natural ecosystem and public health. It is very important to seek environment-friendly and cost-efficient methods to remove MC-LR in water. In this study, the MC-degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the influence of environmental factors including various temperatures, MC concentrations and pH on the MC-degrading activities were investigated utilizing high-performance liquid chromatography (HPLC). Read More

Sci Total Environ 2018 Dec 4;643:1348-1356. Epub 2018 Jul 4.

LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. Electronic address:

Concerns on the presence of emerging contaminants (ECs) in water sources have increased in recent years. The lack of efficient technologies to remove ECs from residual waters contributes for their appearance in drinking water distribution systems (DWDS). Therefore, sessile microorganisms on DWDS pipes are continuously exposed to trace concentrations of ECs. Read More