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J Environ Sci Health C Toxicol Carcinog 2020 Jul 10:1-17. Epub 2020 Jul 10.

Biological Sciences Department, University of Memphis, Memphis, Tennessee, USA.

Triclosan (TCS), a well-studied antimicrobial compound and an environmental pollutant, is present in many household products. A systematic survey of TCS-antibiotic-bacteria interactions is lacking. We wish to understand the origin of such interactions by testing 16 phylogenetically well-characterized bacteria for their sensitivities to 6 different classes of antibiotics with or without the presence of TCS. Read More

Can J Microbiol 2020 Jul 8. Epub 2020 Jul 8.

Gurukul Kangri University, dept of Microbiology , Dept of Microbiology , Gurukul Kangri University , Haridwar , Haridwar, Uttranchal, India , 249404 ;

Non-rhizobial root nodule endophytic bacteria are known to have beneficial effects on host plants and are also considered as contaminants or as opportunists. They grow either individually or as a co-occupant of the root nodules of the legume plants. The isolation of the bacteria was carried out from the root nodules of medicinal legume M. Read More

Front Microbiol 2020 17;11:1273. Epub 2020 Jun 17.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

The presence of extended-spectrum β-lactamase (ESBL)-producing bacteria in environmental sources has been reported worldwide and constitutes a serious risk of community-acquired infections with limited treatment options. The current study aimed to explore the presence of these worrisome bacteria in a pond located at the Norwegian University of Life Sciences in Ås, Norway. A total of 98 bacterial isolates survived growth on selective chromogenic media and were identified by 16S rRNA Sanger sequencing. Read More

Pediatr Pulmonol 2020 Jun 30. Epub 2020 Jun 30.

Centre de ressources et de compétences pour la mucoviscidose adulte, Service de médecine interne et vasculaire, Hospices Civils de Lyon, Lyon, France.

Background: To better understand mechanisms of infection with nontuberculous mycobacteria (NTM) in cystic fibrosis (CF) patients, we explore different risk factors associated with NTM positivity in a meta-analysis.

Methods: Studies published before 31 July 2019 were selected from MEDLINE. Combined odds ratios (ORs) were calculated by pooling the ORs of each study. Read More

BMC Infect Dis 2020 Jun 29;20(1):452. Epub 2020 Jun 29.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an, Shaanxi, 710061, People's Republic of China.

Background: Bacterial infections are the most frequent complications in patients with malignancy, and the epidemiology of nosocomial infections among cancer patients has changed over time. This study aimed to evaluate the characteristics, antibiotic resistance patterns, and prognosis of nosocomial infections due to multidrug-resistant (MDR) bacteria in cancer patients.

Methods: This retrospective observational study analyzed cancer patients with nosocomial infections caused by MDR from August 2013 to May 2019. Read More

J Antimicrob Chemother 2020 Jun 26. Epub 2020 Jun 26.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, China.

Background: SPR206 is a novel polymyxin analogue. Activity against clinical isolates is little documented.

Methods: A collection of 200 MDR, carbapenem-resistant, tigecycline-resistant, colistin-resistant and non-MDR clinical isolates of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae and Stenotrophomonas maltophilia was obtained from 50 centres across China (2016-17). Read More

Eur J Microbiol Immunol (Bp) 2020 Apr 23. Epub 2020 Apr 23.

2Department of Public Health, Faculty of Medicine, University of Szeged, Dóm tér 10., 6720, Szeged,Hungary.

Stenotrophomonas maltophilia is an aerobic, oxidase-negative and catalase-positive bacillus. S. maltophilia is a recognized opportunistic pathogen. Read More

Front Microbiol 2020 3;11:1160. Epub 2020 Jun 3.

Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.

The pathogenicity of is regulated in part by its quorum sensing (QS) system. The main QS signaling molecule in is known as diffusible signal factor (DSF), and the gene cluster is responsible for its synthesis and perception. Two cluster variants have been previously described, -1 and -2, which differ basically in the conditions under which DSF is produced. Read More

BMC Microbiol 2020 Jun 23;20(1):177. Epub 2020 Jun 23.

Department of Pulmonary & Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.

Background: The rapid identification of pathogenic bacteria is important for determining an appropriate antimicrobial therapy for pneumonia, but traditional bacterial culture is time-consuming and labourious. The aim of this study was to develop and evaluate a DNA microarray assay for the simultaneous detection of fifteen bacterial species directly from respiratory tract specimens in patients with pneumonia. These species included Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Mycoplasma pneumoniae, Enterococcus faecalis, Enterococcus faecium, Enterobacter cloacae, Stenotrophomonas maltophilia, Burkholderia cepacia, Legionella pneumophila and Chlamydia pneumoniae. Read More

Antimicrob Agents Chemother 2020 Jun 22. Epub 2020 Jun 22.

College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA

The production of a metallo-(L1) and serine-(L2) β-lactamase precludes the use of β-lactams for the treatment of infections. Pre-clinical data suggest cefiderocol is the first approved β-lactam with reliable activity against but data against strains resistant to current first -line agents are limited and no studies have assessed cefiderocol-based combinations. The objective of this study was to evaluate and compare the activity of cefiderocol alone and in combination with levofloxacin, minocycline, polymyxin B, and trimethoprim-sulfamethoxazole (TMP-SMZ) against a collection of highly resistant clinical isolates. Read More

Ann Transl Med 2020 May;8(9):604

Department of Microbiology, School of Medicine, University of Granada-Instituto de Investigación Biosanitaria, Granada, Spain.

Background: Antibiotic resistance is a serious public health challenge exacerbated by the widespread use of β-lactam and glycopeptide antibiotics. The identification of resistances is crucial, and CHROMID ESBL medium has been developed to detect enterobacteria with extended-spectrum β-lactamases (ESBL). The objective of this study was to evaluate the potential of this medium to detect other types of resistant bacteria. Read More

BMC Microbiol 2020 Jun 19;20(1):170. Epub 2020 Jun 19.

School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.

Background: Stenotrophomonas maltophilia is an emerging nosocomial pathogen that causes infection in immunocompromised patients. S. maltophilia isolates are genetically diverse, contain diverse virulence factors, and are variably pathogenic within several host species. Read More

Microbiol Resour Announc 2020 Jun 18;9(25). Epub 2020 Jun 18.

Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China

Bacteria of the genus are opportunistic and have been documented in the guts of several insect species. Here, we present the complete genome sequence of strain CPBW01, isolated from the wings of the Colorado potato beetle, , collected from potato fields in Urumqi (43.71N, 87. Read More

Infect Drug Resist 2020 28;13:1559-1566. Epub 2020 May 28.

Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Purpose: To study the clinical characteristics and factors associated with mortality of patients who had infections.

Patients And Methods: We conducted a retrospective study to determine the clinical characteristics and factors associated with mortality for infections among hospitalized adult patients at Siriraj Hospital. The clinical and microbiological data were collected from medical records December 2013-December 2016. Read More

Clin Infect Dis 2020 Jun 16. Epub 2020 Jun 16.

Division of Infectious Diseases and Center for Antimicrobial Resistance and Microbial Genomics (CARMiG), UTHealth McGovern Medical School, Houston, TX, USA.

Background: Stenotrophomonas maltophilia is increasingly common in patients with acute myeloid leukemia (AML). Relatively little is known about factors that drive S. maltophilia infection. Read More

Clin Infect Dis 2020 Jun 16. Epub 2020 Jun 16.

Fred Hutchinson Cancer Research Center, Seattle, WA.

Microb Pathog 2020 Jun 10;149:104293. Epub 2020 Jun 10.

Department of Microbiology, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh. Electronic address:

Stenotrophomonas maltophilia is an emerging opportunistic pathogen, and immunocompromised patients are at a higher risk of getting infected with this nosocomial bacterium. The biggest concern is its inherent resistance to most of the commonly used antibiotics, leaving a few options for treatment. Moreover, recent studies have reported the emergence of its resistance to trimethoprim/sulfamethoxazole (TMP/SMX), the drugs of choice against this pathogen. Read More

Enzyme Microb Technol 2020 Aug 7;138:109548. Epub 2020 Apr 7.

State Key Laboratory of Bioreactor Engineering, Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, PR China; Shanghai Collaborative Innovation Center for Biomanufacturing (SCICB), Shanghai, 200237, PR China. Electronic address:

A novel prolyl endopeptidase from Stenotrophomonas maltophilia, SmPEP, was discovered and characterized. The specific activity of the recombinant SmPEP expressed by Escherichia coli BL21 (DE3), was 68.3 U/mg at pH 8. Read More

Ann Pharmacother 2020 Jun 10:1060028020929988. Epub 2020 Jun 10.

Lebanese American University, School of Phramacy, Byblos Campus, Byblos, Lebanon.

This article reviews the available data on the chemistry, spectrum of activity, pharmacokinetic and pharmacodynamic properties, clinical efficacy, and potential place in therapy of cefiderocol. A literature search through PubMed, Google Scholar, and ClinicalTrials.gov was conducted (2009 to March 2020) using the search terms and . Read More

Animals (Basel) 2020 Jun 4;10(6). Epub 2020 Jun 4.

Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.

Selenium (Se) is able to transform from inorganic to organic forms via many bacterial species. This feature is being considered for delivering more bioavailable selenium compounds such as selenocysteine and selenomethionine for human and animal diet. This study investigated the effects of bacterial selenoprotein versus inorganic Se on the carcass characteristics, breast meat selenium content, antioxidant status, and meat quality of broiler chickens. Read More

J Family Med Prim Care 2020 Mar 26;9(3):1407-1412. Epub 2020 Mar 26.

Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.

Respiratory tract infections are the most common diseases that are associated with social burden for the patient. Western Rajasthan has cases of Cystic fibrosis due to migrant population. The dry and dusty environment has led to prevalence of silicosis and COPD. Read More

Lancet Infect Dis 2020 Jun 4. Epub 2020 Jun 4.

Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, MD, USA.

Background: Evidence-based needs assessments for novel antibiotics against highly-resistant Gram-negative infections (GNIs) are scarce. We aimed to use real-world data from an electronic health record repository to identify treatment opportunities in US hospitals for GNIs resistant to all first-line drugs.

Methods: For this retrospective cohort study, population estimates with an unmet need for novel Gram-negative antibiotics were quantified using the Cerner Health Facts database (2009-15), aggregating episodes of infection in US hospitals with pathogens displaying difficult-to-treat resistance (DTR; resistance to carbapenems, other β-lactams, and fluoroquinolones) and episodes involving empirical coverage with reserve drugs (colistin or polymyxin B and aminoglycosides). Read More

IDCases 2020 14;21:e00796. Epub 2020 May 14.

The Department of Orthopaedic Surgery Faculty of Medicine, School of Health Sciences, University of Hospital of Larissa, Larissa, Greece.

is a well-known opportunistic Gram-negative bacterium causing mainly hospital-acquired infections, . We report the first case, to our knowledge, of a periprosthetic infection caused by this pathogen in an artificial joint. has the ability to form biofilm, and subsequently should not be excluded in the investigation of prosthetic joint infections. Read More

mSystems 2020 Jun 2;5(3). Epub 2020 Jun 2.

Centro Nacional de Biotecnología, CSIC, Madrid, Spain

Fosfomycin is a bactericidal antibiotic, analogous to phosphoenolpyruvate, that exerts its activity by inhibiting the activity of MurA. This enzyme catalyzes the first step of peptidoglycan biosynthesis, the transfer of enolpyruvate from phosphoenolpyruvate to uridine-diphosphate--acetylglucosamine. Fosfomycin is increasingly being used, mainly for treating infections caused by Gram-negative multidrug-resistant bacteria. Read More

Am J Case Rep 2020 Jun 2;21:e924577. Epub 2020 Jun 2.

Department of Internal Medicine, Reading Hospital, Tower Health System, Reading, PA, USA.

BACKGROUND This article describes a finding of sputum culture positive for Stenotrophomonas maltophilia in an elderly woman with past medical history of chronic obstructive pulmonary disease (COPD) and hypertension, presenting with acute hypoxemic hypercapnic respiratory failure secondary to COPD exacerbation from bronchitis/bronchopneumonia. CASE REPORT Computed tomography (CT) of the chest showed secretions in the lower lobe bronchi and small scattered clustered nodules consistent with bronchitis/mild bronchopneumonia without evidence of pulmonary embolism. A sputum culture was positive for Stenotrophomonas maltophilia. Read More

Future Microbiol 2020 May 1;15:497-508. Epub 2020 Jun 1.

Department of Pathology, Health Sciences Center, Universidade Federal do Espírito Santo (UFES), Av. Marechal Campos, 1468, 29040-090 Vitória, Espírito Santo, Brazil.

To investigate if the prior use of nontargeted antibiotics induces cross-tolerance in . Antibiotic induction was performed to evaluate daptomycin and vancomycin as possible tolerance-inducing drugs measured by minimum bactericidal concentration/minimum inhibitory concentration (MIC) ratio, adapted disk-diffusion tests and time-kill curves. After antibiotic exposure, three potentially tolerant strains were isolated, maintaining the same MIC value of levofloxacin, with minimum bactericidal concentration/MIC ratio slightly higher than the parental. Read More

Arch Argent Pediatr 2020 06;118(3):e317-e323

Pediatric Infectious Diseases Unit, Department of Pediatrics, Hacettepe University Faculty of Medicine, Ankara, Turkey.

Stenotrophomonas maltophilia is a multidrug-resistant, Gramnegative, and biofilm-forming pathogen. Information is limited concerning S. maltophilia bacteremia in children. Read More

Open Forum Infect Dis 2020 May 22;7(5):ofaa141. Epub 2020 Apr 22.

Infectious Disease Drug Development Consulting LLC, Easton, Connecticut, USA.

Background: is an opportunistic pathogen observed in both nosocomial and community-onset infections. is intrinsically resistant to many currently available broad-spectrum antibiotics and is often not included in antimicrobial resistance surveillance studies or stewardship programs' guidelines.

Methods: A retrospective cohort study of patients with bloodstream infection (BSI) in the United States was conducted using the 2010-2015 US Premier Healthcare Database. Read More

Am J Case Rep 2020 May 25;21:e921466. Epub 2020 May 25.

Department of Internal Medicine, University of Pittsburgh Medical Center (UPMC) Pinnacle, Harrisburg, PA, USA.

BACKGROUND Stenotrophomonas maltophilia has the propensity to cause a plethora of opportunistic infections in humans owing to biofilm formation and antibiotic resistance. It is often seen as a co-organism along with Pseudomonas aeruginosa. CASE REPORT A 70-year-old woman with several co-morbidities presented reporting hypoglycemia and dyspnea. Read More

Transplant Proc 2020 May 20. Epub 2020 May 20.

Silesian Center for Heart Diseases, Zabrze Poland; Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, Medical University of Silesia in Katowice, Zabrze, Poland.

Background: Lung transplantation (LTx) is the only treatment for patients with end-stage lung disease. This procedure is associated with a risk of complications related to airway stenosis, which can be treated by means of bronchoscopic interventions (BI). Microbiological colonization may have an impact on airway complications. Read More

Mar Drugs 2020 May 20;18(5). Epub 2020 May 20.

Institute of Biochemistry and Cell Biology, National Research Council, 80131 Naples, Italy.

Rhamnolipids (RLs) are surface-active molecules mainly produced by Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacteria isolated from Edmonson Point Lake, Ross Sea, Antarctica were subjected to preliminary screening for the biosurfactant activity. The positive strains were identified by 16S rRNA gene sequencing and the produced RLs were characterized by liquid chromatography coupled to high resolution mass spectrometry (LC-HRESIMS) and liquid chromatography coupled with tandem spectrometry (LC-MS/MS), resulting in a new mixture of 17 different RL congeners, with six previously undescribed RLs. Read More

Chemosphere 2020 Jul 27;251:126310. Epub 2020 Feb 27.

Department of Chemistry and Biology "A. Zambelli", University of Salerno, Fisciano, SA, Italy.

Arsenic-(As) pollution is an increasing threat across the globe and it is reaching harmful values in several areas of the world. In this perspective, we assayed bio-phyto-remediation technology using Arundo donax L., assisted by Plant Growth Promoting Bacteria (PGPB) consortium (BC) constituted of two strains of Stenotrophomonas maltophilia sp. Read More

Sci Total Environ 2020 Aug 1;732:139125. Epub 2020 May 1.

Division of Coastal Sciences, School of Ocean Science and Engineering, The University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS 39564, United States of America. Electronic address:

Aquatic contamination, oil spills in particular, could lead to the accumulation of antibiotic resistance by promoting selection for and/or transfer of resistance genes. However, there have been few studies on antibiotic resistance in marine mammals in relation to environmental disturbances, specifically oil contaminations. Here we initiated a study on antibiotic resistance bacteria in bottlenose dolphins Tursiops truncatus in relation to oil contamination following the 2010 BP Oil Spill in the northern Gulf of Mexico. Read More

J Antimicrob Chemother 2020 May 14. Epub 2020 May 14.

Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.

Background: Formaldehyde toxicity is invariably stressful for microbes. Stenotrophomonas maltophilia, a human opportunistic pathogen, is widely distributed in different environments and has evolved an array of systems to alleviate various stresses.

Objectives: To characterize the role of the formaldehyde detoxification system FadRACB of S. Read More

J Clin Pharm Ther 2020 Aug 14;45(4):836-839. Epub 2020 May 14.

Department of Clinical Pharmacy and Translational Sciences, University of Tennessee Health Science Center, Memphis, Tennessee.

What Is Known And Objective: Stenotrophomonas maltophilia is an intrinsically multidrug-resistant (MDR) organism which commonly presents as a respiratory tract infection. S. maltophilia is typically treated with high-dose sulfamethoxazole/trimethoprim (SMX/TMP). Read More

Nat Commun 2020 May 7;11(1):2258. Epub 2020 May 7.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

En route to a bio-based chemical industry, the conversion of fatty acids into building blocks is of particular interest. Enzymatic routes, occurring under mild conditions and excelling by intrinsic selectivity, are particularly attractive. Here we report photoenzymatic cascade reactions to transform unsaturated fatty acids into enantiomerically pure secondary fatty alcohols. Read More

Microb Drug Resist 2020 May 4. Epub 2020 May 4.

Infectious Diseases Department, University Hospital of Trieste, Trieste, Italy.

Infections caused by metallo-β-lactamase (MBL)-producing bacteria are emerging and carry a significant impact on patients' outcome. MBL producers are spread worldwide, both in community and hospital setting, with increasingly reported epidemic clusters and the search for MBL inhibitors is an important topic for public health. MBLs are zinc-dependent enzymes whose functioning can be hampered by zinc chelators. Read More

Can J Infect Dis Med Microbiol 2020 9;2020:3948719. Epub 2020 Apr 9.

Department of Clinical Laboratory, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China.

Objective: This study aimed to detect 5 kinds of genes related to plasmid-mediated quinolone resistance in four species of nonfermenting bacteria with 2 drug resistance phenotypes (multidrug resistance and pandrug resistance), which were (Ab), (Pa), (Sm), and (Em).

Methods: The Phoenix NMIC/ID-109 panel and API 20NE panel were applied to 19 isolated strains, including 6 Ab strains (2 strains with multidrug resistance and 4 strains with pandrug resistance), 6 Pa strains (3 strains with multidrug resistance and 3 strains with pandrug resistance), 4 Sm strains (2 strains with multidrug resistance and 2 strains with pandrug resistance), and 3 Cm strains (2 strains with multidrug resistance and 1 strain with pandrug resistance). After strain identification and drug susceptibility test, PCR was applied to detect 5 genes related to plasmid-mediated quinolone resistance. Read More

Nat Commun 2020 Apr 27;11(1):2044. Epub 2020 Apr 27.

Molecular and Experimental Mycobacteriology, Research Center Borstel, Borstel, Germany.

Recent studies portend a rising global spread and adaptation of human- or healthcare-associated pathogens. Here, we analyse an international collection of the emerging, multidrug-resistant, opportunistic pathogen Stenotrophomonas maltophilia from 22 countries to infer population structure and clonality at a global level. We show that the S. Read More

Diagnostics (Basel) 2020 Apr 15;10(4). Epub 2020 Apr 15.

Dipartimento di Medicina Molecolare e Biotecnologie Mediche, University of Naples Federico II, 80131 Naples, Italy.

About 50% of patients with cystic fibrosis (CF) have sinonasal complications, which include inferior turbinate hypertrophy (NTH) and/or nasal polyposis (NP), and different degrees of lung disease, which represents the main cause of mortality. Monitoring of sinonasal disease requires complex instrumental procedures, while monitoring of lung inflammation requires invasive collection of bronchoalveolar lavage fluid. The aim of this study was to investigate the associations between salivary cytokines levels and CF-related airway diseases. Read More

J Clin Microbiol 2020 Jun 24;58(7). Epub 2020 Jun 24.

Division of Laboratory and Genomic Medicine, Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA

Our objective was to evaluate the diagnostic yield and accuracy of the BioFire FilmArray pneumonia panel (BFPP) for identification of pathogens in lower respiratory tract specimens ( = 200) from emergency department (ED) and intensive care unit (ICU) patients at a tertiary care academic medical center. Specimens were collected between January and November 2018, from patients ≥18 years of age, and culture was performed as part of standard-of-care testing. The BFPP identified a viral or bacterial target in 117/200 (58. Read More

BMJ Case Rep 2020 Apr 20;13(4). Epub 2020 Apr 20.

HRB Clinical Research Facility, University College Cork, Cork, Ireland

Cystic fibrosis (CF) is the most common life-limiting autosomal recessive genetic disorder among Caucasian populations. The majority of CF cases are diagnosed in childhood; however, increasing numbers of adults are being diagnosed with the condition. We present the case of a 65-year-old Irish woman presenting with a chronic cough and a history of recurrent respiratory tract infections. Read More

J Glob Antimicrob Resist 2020 Apr 18;22:206-209. Epub 2020 Apr 18.

Guizhou University, Guiyang 550025, China.

Objectives A multidrug-resistant bacterium, Stenotrophomonas sp. SXG-1, was isolated from the liver of diseased hybrid sturgeon from Guizhou province, China. Methods Whole-genome sequencing was performed on the Illumina HiSeq 2500-PE125 platform with MPS (massively parallel sequencing) Illumina technology. Read More

New Microbiol 2020 Apr 19;43(2):82-88. Epub 2020 Apr 19.

The Key Laboratory of Modern Toxicology of Ministry of Education and department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing, 211166, China.

In order to investigate molecular typing and fatty acid methyl esters (FAMEs) typing of clinical Stenotrophomonas maltophilia (S.maltophilia) isolates based on Random Amplification Polymorphic DNA (RAPD) and Gas Chromatography-Mass Spectrometer (GC-MS) methods, we collected 35 drug-resistant S. maltophilia isolates from March to December 2017 in a comprehensive hospital. Read More

AMB Express 2020 Apr 15;10(1):71. Epub 2020 Apr 15.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt.

An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experimentally for its catalytic properties and by bioinformatics analysis for its immunogenicity. Read More

J Antimicrob Chemother 2020 Jul;75(7):1840-1849

UGC Enfermedades Infecciosas, Microbiología Clínica y Medicina Preventiva, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen Macarena/CSIC/Universidad de Sevilla, Sevilla, Spain.

Background: Cefiderocol is a novel siderophore cephalosporin, developed for activity against MDR Gram-negative bacilli (MDR-GNB).

Objectives: To assess the in vitro antibacterial activity of cefiderocol against a collection of MDR-GNB clinical isolates from hospitals in southern Spain.

Methods: Two hundred and thirty-one isolates of successful clones were tested: 125 Enterobacterales (121 ESBL- and/or carbapenemase-producing Klebsiella pneumoniae and 4 carbapenemase-producing Enterobacter cloacae), 80 Acinetobacter baumannii, 6 Pseudomonas aeruginosa and 20 Stenotrophomonas maltophilia. Read More

QJM 2020 Apr 10. Epub 2020 Apr 10.

Royal Infirmary and University of Edinburgh, 51 Little France Crescent, Edinburgh, EH16 4SA, Scotland, UK.

Background: Stenotrophomonas maltophilia is a bacteria whose role in patients with cystic fibrosis bronchiectasis has been previously studied; little is known about its role in non-CF bronchiectasis.

Aim: Investigate the risk factors for S. Maltophilia acquisition and its clinical impact on bronchiectasis patients. Read More

J Nat Prod 2020 May 10;83(5):1495-1504. Epub 2020 Apr 10.

Institute of Biochemistry and Cellular Biology, National Research Council (IBBC-CNR), Via Pietro Castellino 111, I-80131 Naples, Italy.

A wide range of prescreening tests for antimicrobial activity of 59 bacterial isolates from sediments of Ria Formosa Lagoon (Algarve, Portugal) disclosed 3.6 as the most active antibacterial producing strain. This bacterial strain, which has not previously been submitted for chemical profiling, was subjected to whole genome sequencing, which aided in the discovery and elucidation of a prodigiosin biosynthetic gene cluster that was predicted by the bioinformatic tool KEGG BlastKoala. Read More

Oman Med J 2020 Mar 30;35(2):e109. Epub 2020 Mar 30.

Department of Hematology and Immunology, Faculty of Basic Medical Science, College of Health Sciences, Obafemi Awolowo University, Ile-Ife, Osun-State, Nigeria.

Objectives: We sought to determine the prevalence of asymptomatic bacteriuria (ASB) in patients with sickle cell disease (SCD), the susceptibility profile of its agents and their extended-spectrum β-lactamase (ESBL) production.

Methods: Fifty-nine patients with SCD and 116 healthy controls were investigated. Urine samples were collected and cultured by standard techniques. Read More

J Microbiol Methods 2020 May 30;172:105911. Epub 2020 Mar 30.

INRAe, Université de Montpellier, UMR1333-DGIMI, 34095 Montpellier Cedex 05, France. Electronic address:

Pseudomonas protegens is a rhizosphere pseudomonad with a high agronomical potential (entomopathogenic and beneficial to plants) and bio-catalytic activities, but no selective medium has been described for its isolation. We developed a semi-selective minimum agar medium for the specific isolation and growth of P. protegens. Read More