1,119 results match your criteria Stem cells translational medicine[Journal]


Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments.

Stem Cells Transl Med 2019 Apr 19. Epub 2019 Apr 19.

University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa, USA.

Subretinal delivery of stem cell-derived retinal cells as a strategy to treat retinal degenerative blindness holds great promise. Currently, two clinical trials are underway in which human fetal retinal progenitor cells (RPCs) are being delivered to patients by intravitreal or subretinal injection to preserve or restore vision, respectively. With the advent of the induced pluripotent stem cell (iPSC), and in turn three-dimensional derivation of retinal tissue, it is now possible to generate autologous RPCs for cell replacement. Read More

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http://dx.doi.org/10.1002/sctm.18-0210DOI Listing

Generation of a Functioning and Self-Renewing Diaphragmatic Muscle Construct.

Stem Cells Transl Med 2019 Apr 10. Epub 2019 Apr 10.

Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.

Surgical repair of large muscular defects requires the use of autologous graft transfer or prosthetic material. Naturally derived matrices are biocompatible materials obtained by tissue decellularization and are commonly used in clinical practice. Despite promising applications described in the literature, the use of acellular matrices to repair large defects has been only partially successful, highlighting the need for more efficient constructs. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/sctm.18-0206
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http://dx.doi.org/10.1002/sctm.18-0206DOI Listing
April 2019
4 Reads

Bone Marrow Mesenchymal Stromal Cells in Patients with Osteoarthritis Results in Overall Improvement in Pain and Symptoms and Reduces Synovial Inflammation.

Stem Cells Transl Med 2019 Apr 9. Epub 2019 Apr 9.

Arthritis Program, University Health Network, Toronto, Ontario, Canada.

Patients with late-stage Kellgren-Lawrence knee osteoarthritis received a single intra-articular injection of 1, 10, or 50 million bone marrow mesenchymal stromal cells (BM-MSCs) in a phase I/IIa trial to assess safety and efficacy using a broad toolset of analytical methods. Besides safety, outcomes included patient-reported outcome measures (PROMs): Knee Injury and Osteoarthritis Outcome Score (KOOS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC); contrast-enhanced magnetic resonance imaging (MRI) for cartilage morphology (Whole Organ MRI Scores [WORMS]), collagen content (T2 scores), and synovitis; and inflammation and cartilage turnover biomarkers, all over 12 months. BM-MSCs were characterized by a panel of anti-inflammatory markers to predict clinical efficacy. Read More

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http://dx.doi.org/10.1002/sctm.18-0183DOI Listing

Use of Bone Marrow-Derived Mesenchymal Stem Cells in Prostate Cancer Could Increase the Risk of Cancer Progression.

Stem Cells Transl Med 2019 Mar 29. Epub 2019 Mar 29.

Regenerative Medicine Laboratory, Dr. D.Y. Patil Vidyapeeth, Pune, India.

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https://onlinelibrary.wiley.com/doi/abs/10.1002/sctm.19-0050
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http://dx.doi.org/10.1002/sctm.19-0050DOI Listing
March 2019
2 Reads
3.596 Impact Factor

Accumulating Transcriptome Drift Precedes Cell Aging in Human Umbilical Cord-Derived Mesenchymal Stromal Cells Serially Cultured to Replicative Senescence.

Stem Cells Transl Med 2019 Mar 28. Epub 2019 Mar 28.

Aurora BioSolutions Inc., Medicine Hat, Alberta, Canada.

In preclinical studies, mesenchymal stromal cells (MSCs) exhibit robust potential for numerous applications. To capitalize on these benefits, cell manufacturing and delivery protocols have been scaled up to facilitate clinical trials without adequately addressing the impact of these processes on cell utility nor inevitable regulatory requirements for consistency. Growing evidence indicates that culture-aged MSCs, expanded to the limits of replicative exhaustion to generate human doses, are not equivalent to early passage cells, and their use may underpin reportedly underwhelming or inconsistent clinical outcomes. Read More

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http://dx.doi.org/10.1002/sctm.18-0246DOI Listing
March 2019
1 Read

Extracellular Vesicles Released by Allogeneic Human Cardiac Stem/Progenitor Cells as Part of their Therapeutic Benefit.

Stem Cells Transl Med 2019 Mar 28. Epub 2019 Mar 28.

Institut National de la Santé et de la Recherche Médicale (INSERM) UMRS-976, Université Paris-Diderot, Hôpital Saint-Louis, Paris, France.

The positive effects of therapeutic human allogeneic cardiac stem/progenitor cells (hCPC) in terms of cardiac repair/regeneration are very likely mediated by paracrine effects. Our previous studies revealed the advantageous immune interactions of allogeneic hCPC and proposed them as part of the positive paracrine effects occurring upon their application postmyocardial infarction (MI). Currently, extracellular vesicles/exosomes (EV/Exs) released by stem/progenitor cells are also proposed as major mediators of paracrine effects of therapeutic cells. Read More

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http://dx.doi.org/10.1002/sctm.18-0256DOI Listing
March 2019
2 Reads
3.596 Impact Factor

Concise Review: Patency of Small-Diameter Tissue-Engineered Vascular Grafts: A Meta-Analysis of Preclinical Trials.

Stem Cells Transl Med 2019 Mar 28. Epub 2019 Mar 28.

Laboratory of Molecular and Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense C, Denmark.

Several patient groups undergoing small-diameter (<6 mm) vessel bypass surgery have limited autologous vessels for use as grafts. Tissue-engineered vascular grafts (TEVG) have been suggested as an alternative, but the ideal TEVG remains to be generated, and a systematic overview and meta-analysis of clinically relevant studies is lacking. We systematically searched PubMed and Embase databases for (pre)clinical trials and identified three clinical and 68 preclinical trials ([>rabbit]; 873 TEVGs) meeting the inclusion criteria. Read More

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http://dx.doi.org/10.1002/sctm.18-0287DOI Listing

Bioconjugated Carbon Dots for Delivery of siTnfα to Enhance Chondrogenesis of Mesenchymal Stem Cells by Suppression of Inflammation.

Stem Cells Transl Med 2019 Mar 28. Epub 2019 Mar 28.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People's Republic of China.

Although a promising strategy, the mesenchymal stem cell (MSC)-based therapy of cartilage defects is sometimes accompanied with chronic inflammation during the remodeling status, which may hinder cartilage regeneration. During this process, the inflammatory cytokine tumor necrosis factor α (TNFα) plays an important role and may be a potential target. In this study, we investigated the effect of Tnfα RNA interference by introducing a functional and highly safe carbon dot (CD)-SMCC nanovector synthesized by bioconjugation of CDs with a protein crosslinker, sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC), as the vehicle of the silenced TNFα (siTnfα) on chondrogenesis of MSCs. Read More

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http://dx.doi.org/10.1002/sctm.18-0289DOI Listing

Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences.

Stem Cells Transl Med 2019 Mar 27. Epub 2019 Mar 27.

Institute of Genetic Medicine, Newcastle University, Newcastle, United Kingdom.

A major goal in the stem cell field is to generate tissues which can be utilized as a universal tool for in vitro models of development and disease, drug development, or as a resource for patients suffering from disease or injury. Great efforts are being made to differentiate human pluripotent stem cells in vitro toward retinal tissue, which is akin to native human retina in its cytoarchitecture and function, yet the numerous existing retinal induction protocols remain variable in their efficiency and do not routinely produce morphologically or functionally mature photoreceptors. Herein, we determine the impact that the method of embryoid body (EB) formation and maintenance as well as cell line background has on retinal organoid differentiation from human embryonic stem cells and human induced pluripotent stem cells. Read More

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http://dx.doi.org/10.1002/sctm.18-0267DOI Listing
March 2019
2 Reads

Early Intravenous Infusion of Mesenchymal Stromal Cells Exerts a Tissue Source Age-Dependent Beneficial Effect on Neurovascular Integrity and Neurobehavioral Recovery After Traumatic Cervical Spinal Cord Injury.

Stem Cells Transl Med 2019 Mar 26. Epub 2019 Mar 26.

Division of Genetics and Development, Krembil Research Institute, University Health Network, Toronto, Ontario, Canada.

Localized vascular disruption after traumatic spinal cord injury (SCI) triggers a cascade of secondary events, including inflammation, gliosis, and scarring, that can further impact recovery. In addition to immunomodulatory and neurotrophic properties, mesenchymal stromal cells (MSCs) possess pericytic characteristics. These features make MSCs an ideal candidate for acute cell therapy targeting vascular disruption, which could reduce the severity of secondary injury, enhance tissue preservation and repair, and ultimately promote functional recovery. Read More

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http://dx.doi.org/10.1002/sctm.18-0192DOI Listing
March 2019
4 Reads

A Preview of Selected Articles.

Stem Cells Transl Med 2019 Apr;8(4):317-319

Centro de Investigación Príncipe Felipe, Valencia, Spain.

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http://dx.doi.org/10.1002/sctm.19-0065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431732PMC
April 2019
1 Read

Progress in the Use of Induced Pluripotent Stem Cell-Derived Neural Cells for Traumatic Spinal Cord Injuries in Animal Populations: Meta-Analysis and Review.

Stem Cells Transl Med 2019 Mar 22. Epub 2019 Mar 22.

Department of Biological Sciences, Oakland University College of Arts and Sciences, Rochester, Michigan, USA.

Induced pluripotent stem cells (iPSCs) are cells genetically reprogrammed from somatic cells, which can be differentiated into neurological lineages with the aim to replace or assist damaged neurons in the treatment of spinal cord injuries (SCIs) caused by physical trauma. Here, we review studies addressing the functional use of iPSC-derived neural cells in SCIs and perform a meta-analysis to determine if significant motor improvement is restored after treatment with iPSC-derived neural cells compared with treatments using embryonic stem cell (ESC)-derived counterpart cells and control treatments. Overall, based on locomotion scales in rodents and monkeys, our meta-analysis indicates a therapeutic benefit for SCI treatment using neural cells derived from either iPSCs or ESCs, being this of importance due to existing ethical and immunological complications using ESCs. Read More

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http://dx.doi.org/10.1002/sctm.18-0225DOI Listing

EP Antagonist-Elicited Extracellular Vesicles from Mesenchymal Stem Cells Rescue Cognition/Learning Deficiencies by Restoring Brain Cellular Functions.

Stem Cells Transl Med 2019 Mar 19. Epub 2019 Mar 19.

Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli, Taiwan, Republic of China.

Adult brains have limited regenerative capacity. Consequently, both brain damage and neurodegenerative diseases often cause functional impairment for patients. Mesenchymal stem cells (MSCs), one type of adult stem cells, can be isolated from various adult tissues. Read More

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http://dx.doi.org/10.1002/sctm.18-0284DOI Listing
March 2019
1 Read

Concise Review: Application of Chemically Modified mRNA in Cell Fate Conversion and Tissue Engineering.

Stem Cells Transl Med 2019 Mar 19. Epub 2019 Mar 19.

Department of Surgery, Weill Cornell Medicine, Cornell University, New York, New York, USA.

Chemically modified RNA (cmRNA) has potential as a safe and efficient tool for nucleic acid-based therapies and regenerative medicine. Modifications in the chemistry of mRNA can enhance stability, reduce immunogenicity, and thus facilitate mRNA-based nucleic acid therapy, which eliminates risk of insertional mutagenesis. In addition to these valuable advantages, the mRNA-based method showed significantly higher efficacy for reprogramming somatic cells to pluripotency compared with DNA- or protein-based methods. Read More

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http://dx.doi.org/10.1002/sctm.18-0259DOI Listing
March 2019
3.596 Impact Factor

Concise Review: BOOSTING T Cell Reconstitution Following Allogeneic Transplantation-Current Concepts and Future Perspectives.

Stem Cells Transl Med 2019 Mar 18. Epub 2019 Mar 18.

Laboratory of Human Lymphohematopoiesis, INSERM UMR 1163, Imagine Institute, Paris, France.

Allogeneic hematopoietic stem cell transplantation (HSCT) is the treatment of choice for a large number of malignant and nonmalignant (inherited) diseases of the hematopoietic system. Nevertheless, non-HLA identical transplantations are complicated by a severe T cell immunodeficiency associated with a high rate of infection, relapse and graft-versus-host disease. Initial recovery of T cell immunity following HSCT relies on peripheral expansion of memory T cells mostly driven by cytokines. Read More

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http://dx.doi.org/10.1002/sctm.18-0248DOI Listing
March 2019
1 Read

Urea-De-Epithelialized Human Amniotic Membrane for Ocular Surface Reconstruction.

Stem Cells Transl Med 2019 Mar 13. Epub 2019 Mar 13.

Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.

The conjunctiva is a clear tissue covering the white part of the eye and lines the back of the eyelids. Conjunctival diseases, such as symblepharon, cause inflammation, discharges, and photophobia. The treatment often requires excision of large parts of conjunctiva. Read More

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http://dx.doi.org/10.1002/sctm.18-0201DOI Listing
March 2019
1 Read

Enabling Large-Scale ex vivo Production of Megakaryocytes from CD34 Cells Using Gas-Permeable Surfaces.

Stem Cells Transl Med 2019 Mar 8. Epub 2019 Mar 8.

Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.

Patients suffering from acute or sustained thrombocytopenia require platelet transfusions, which are entirely donor-based and limited by challenges related to storage and fluctuating supply. Developing cell-culture technologies will enable ex vivo and donor-independent platelet production. However, critical advancements are needed to improve scalability and increase megakaryocyte (Mk) culture productivity. Read More

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http://dx.doi.org/10.1002/sctm.18-0160DOI Listing
March 2019
2 Reads

Long-Overdue Guidelines for the Cord Blood Banking Community.

Authors:
Hal E Broxmeyer

Stem Cells Transl Med 2019 Apr 7;8(4):320-322. Epub 2019 Mar 7.

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.

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http://dx.doi.org/10.1002/sctm.19-0056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431605PMC

Intra-Articular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis: A Phase IIb, Randomized, Placebo-Controlled Clinical Trial.

Stem Cells Transl Med 2019 Mar 5. Epub 2019 Mar 5.

Department of Radiology, Kyung Hee University Hospital at Gangdong, Seoul, South Korea.

Mesenchymal stem cells (MSCs) have been the focus of an emerging treatment for osteoarthritis. However, few studies reported about outcomes of an intra-articular injection of autologous adipose-derived mesenchymal stem cells (AD-MSCs). This study aimed to assess the efficacy and safety of a single intra-articular injection of AD-MSCs for patients with knee osteoarthritis. Read More

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http://dx.doi.org/10.1002/sctm.18-0122DOI Listing
March 2019
3 Reads

A Preview of Selected Articles.

Stem Cells Transl Med 2019 Mar;8(3):205-208

Centro de Investigación Príncipe Felipe, Valencia, Spain.E-mail:

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http://dx.doi.org/10.1002/sctm.19-0034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392340PMC

Stem-Cell Therapy for Esophageal Anastomotic Leakage by Autografting Stromal Cells in Fibrin Scaffold.

Stem Cells Transl Med 2019 Feb 27. Epub 2019 Feb 27.

Institute of Cardiothoracic Surgery at Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.

Esophageal anastomotic leakage (EAL) is a devastating complication for esophagectomy but the available therapies are unsatisfactory. Due to the healing effects of mesenchymal stromal cells (MSCs) and supporting capability of fibrin scaffold (FS), we evaluated the efficacy of a stem-cell therapy for EAL by engrafting adult and autologous MSCs (AAMSCs) in FS and investigated the potential mechanism. Twenty-one rabbits were assigned to AAMSC/FS group (n = 12) and control group (n = 9). Read More

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http://dx.doi.org/10.1002/sctm.18-0137DOI Listing
February 2019
1 Read
3.596 Impact Factor

Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes.

Stem Cells Transl Med 2019 Feb 26. Epub 2019 Feb 26.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

Stem cell-derived exosomes have exhibited promise for applications in tissue regeneration. However, one major problem for stem cell-derived exosome therapies is identifying appropriate source cells. In the present study, we aimed to compare the bone regenerative effect of exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) derived from type 1 diabetes rats (dBMSC-exos) and exosomes secreted by BMSCs derived from normal rats (nBMSC-exos). Read More

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http://dx.doi.org/10.1002/sctm.18-0199DOI Listing
February 2019

A Combination of Intrathecal and Intramuscular Application of Human Mesenchymal Stem Cells Partly Reduces the Activation of Necroptosis in the Spinal Cord of SOD1 Rats.

Stem Cells Transl Med 2019 Feb 25. Epub 2019 Feb 25.

Institute of Experimental Medicine, Czech Academy of Science, Prague, Czech Republic.

An increasing number of studies have demonstrated the beneficial effects of human mesenchymal stem cells (hMSC) in the treatment of amyotrophic lateral sclerosis (ALS). We compared the effect of repeated intrathecal applications of hMSC or their conditioned medium (CondM) using lumbar puncture or injection into the muscle (quadriceps femoris), or a combination of both applications in symptomatic SOD1 rats. We further assessed the effect of the treatment on three major cell death pathways (necroptosis, apoptosis, and autophagy) in the spinal cord tissue. Read More

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http://dx.doi.org/10.1002/sctm.18-0223DOI Listing
February 2019
2 Reads

Neutrophils Derived from Genetically Modified Human Induced Pluripotent Stem Cells Circulate and Phagocytose Bacteria In Vivo.

Stem Cells Transl Med 2019 Feb 21. Epub 2019 Feb 21.

Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.

Bacterial and fungal infections are a major cause of morbidity and mortality in neutropenic patients. Donor derived neutrophil transfusions have been used for prophylaxis or treatment for infection in neutropenic patients. However, the short half-life and the limited availability of large numbers of donor derived neutrophils for transfusion remain a significant hurdle in the implementation of neutrophil transfusion therapy. Read More

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http://dx.doi.org/10.1002/sctm.18-0255DOI Listing
February 2019

Concise Review: Regulation of Self-Renewal in Normal and Malignant Hematopoietic Stem Cells by Krüppel-Like Factor 4.

Stem Cells Transl Med 2019 Feb 21. Epub 2019 Feb 21.

Department Pathology & Immunology, Baylor College of Medicine, Texas Children's Hospital, Houston, Texas, USA.

Pluripotent and tissue-specific stem cells, such as blood-forming stem cells, are maintained through a balance of quiescence, self-renewal, and differentiation. Self-renewal is a specialized cell division that generates daughter cells with the same features as the parental stem cell. Although many factors are involved in the regulation of self-renewal, perhaps the most well-known factors are members of the Krüppel-like factor (KLF) family, especially KLF4, because of the landmark discovery that this protein is required to reprogram somatic cells into induced pluripotent stem cells. Read More

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http://dx.doi.org/10.1002/sctm.18-0249DOI Listing
February 2019
3.596 Impact Factor

Bone Morphogenetic Protein-6 Attenuates Type 1 Diabetes Mellitus-Associated Bone Loss.

Stem Cells Transl Med 2019 Feb 19. Epub 2019 Feb 19.

Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Patients with type 1 diabetes mellitus (T1DM) often suffer from osteopenia or osteoporosis. Although most agree that T1DM-induced hyperglycemia is a risk factor for progressive bone loss, the mechanisms for the link between T1DM and bone loss still remain elusive. In this study, we found that bone marrow-derived mesenchymal stem cells (BMSCs) isolated from T1DM donors were less inducible for osteogenesis than those from non-T1DM donors and further identified a mechanism involving bone morphogenetic protein-6 (BMP6) that was produced significantly less in BMSCs derived from T1DM donors than that in control cells. Read More

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http://dx.doi.org/10.1002/sctm.18-0150DOI Listing
February 2019
2 Reads

Fructose 1,6-Bisphosphate as a Protective Agent for Experimental Fat Grafting.

Stem Cells Transl Med 2019 Feb 18. Epub 2019 Feb 18.

Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.

Fat grafting procedures are considered to be a promising regenerative, cell-directed therapy; however, their survival is mainly influenced by ischemia condition. Fructose 1,6-bisphosphate (FBP), as an intermediate in energy metabolism, has the potential to rescue cells and tissues from hypoxic-ischemic circumstances. In the present study, human lipoaspirates were grafted subcutaneously into nude mice followed by a daily intraperitoneal injection of FBP at different doses for 7 days. Read More

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http://dx.doi.org/10.1002/sctm.18-0212DOI Listing
February 2019
3.596 Impact Factor

Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions.

Stem Cells Transl Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, USA.

Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e. Read More

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http://dx.doi.org/10.1002/sctm.18-0189DOI Listing
February 2019
4 Reads

In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads.

Stem Cells Transl Med 2019 Feb 14. Epub 2019 Feb 14.

Biotechnology and Radiobiology Laboratory, Hadassah - Hebrew University Medical Center, Sharett Institute of Oncology, Jerusalem, Israel.

Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. Read More

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http://dx.doi.org/10.1002/sctm.18-0129DOI Listing
February 2019
1 Read

Concise Review: Update on Retinal Pigment Epithelium Transplantation for Age-Related Macular Degeneration.

Stem Cells Transl Med 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacology, Physiology, and Neuroscience, Rutgers-New Jersey Medical School, Newark, New Jersey, USA.

Retinal cell therapy can have the objectives of rescue (i.e., modulation of metabolic abnormalities primarily for sight preservation) as well as replacement (i. Read More

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http://dx.doi.org/10.1002/sctm.18-0282DOI Listing
February 2019
4 Reads

A Phase I Study to Assess the Safety and Cancer-Homing Ability of Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Men with Localized Prostate Cancer.

Stem Cells Transl Med 2019 Feb 8. Epub 2019 Feb 8.

Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Animal models show that systemically administered bone marrow-derived mesenchymal stem cells (MSCs) home to sites of primary and metastatic prostate cancer (PC)-making them candidates to selectively deliver cytotoxic agents. To further assess this potential as a cell-based therapeutic vehicle, a phase I study testing homing of systemically infused allogeneic MSCs preprostatectomy was conducted. The primary objective was to assess safety and feasibility and to determine if MSCs accumulate within primary PC tissue. Read More

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http://doi.wiley.com/10.1002/sctm.18-0230
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http://dx.doi.org/10.1002/sctm.18-0230DOI Listing
February 2019
7 Reads

Concise Review: Skeletal Muscle as a Delivery Route for Mesenchymal Stromal Cells.

Stem Cells Transl Med 2019 Feb 5. Epub 2019 Feb 5.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada.

Mesenchymal stromal cells (MSCs) have demonstrated extensive capacity to modulate a catabolic microenvironment toward tissue repair. The fate, biodistribution, and dwell-time of the in vivo delivered MSCs largely depend on the choice of the cell delivery route. Intramuscular (IM) delivery of MSCs is clinically safe and has been used for the effective treatment of local pathologies. Read More

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http://dx.doi.org/10.1002/sctm.18-0208DOI Listing
February 2019

Concise Review: Canine Diabetes Mellitus as a Translational Model for Innovative Regenerative Medicine Approaches.

Stem Cells Transl Med 2019 Feb 5. Epub 2019 Feb 5.

Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.

Diabetes mellitus (DM) is a common spontaneous endocrine disorder in dogs, which is defined by persistent hyperglycemia and insulin deficiency. Like type 1 diabetes (T1D) in people, canine DM is a complex and multifactorial disease in which genomic and epigenomic factors interact with environmental cues to induce pancreatic β-cell loss and insulin deficiency, although the pathogenesis of canine DM is poorly defined and the role of autoimmunity is further controversial. Both diseases are incurable and require life-long exogenous insulin therapy to maintain glucose homeostasis. Read More

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http://dx.doi.org/10.1002/sctm.18-0163DOI Listing
February 2019
3 Reads

Metabolic Syndrome Interferes with Packaging of Proteins within Porcine Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Stem Cells Transl Med 2019 Feb 1. Epub 2019 Feb 1.

Divisions of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota, USA.

Mesenchymal stem/stromal cells (MSCs) release extracellular vesicles (EVs), which shuttle proteins to recipient cells, promoting cellular repair. We hypothesized that cardiovascular risk factors may alter the pattern of proteins packed within MSC-derived EVs. To test this, we compared the protein cargo of EVs to their parent MSCs in pigs with metabolic syndrome (MetS) and Lean controls. Read More

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http://dx.doi.org/10.1002/sctm.18-0171DOI Listing
February 2019
3 Reads
3.596 Impact Factor

Intranasal Administration of Extracellular Vesicles Derived from Human Teeth Stem Cells Improve Motor Symptoms and Normalize Tyrosine Hydroxylase Expression in the Substantia Nigra and Striatum of the 6-Hydroxydopamine-Treated Rats.

Stem Cells Transl Med 2019 Feb 1. Epub 2019 Feb 1.

Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.

Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting millions of people worldwide. At present, there is no effective cure for PD, treatments are symptomatic and do not halt progression of neurodegeneration. Extracellular vesicles (EVs) can cross the blood-brain barrier and represent promising alternative to the classical treatment strategies. Read More

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http://dx.doi.org/10.1002/sctm.18-0162DOI Listing
February 2019

Allogeneic Ex Vivo Expanded Corneal Epithelial Stem Cell Transplantation: A Randomized Controlled Clinical Trial.

Stem Cells Transl Med 2019 Apr 28;8(4):323-331. Epub 2019 Jan 28.

Princess Alexandra Eye Pavillion, Edinburgh, United Kingdom.

Limbal stem cell deficiency (LSCD) is a disease resulting from the loss or dysfunction of epithelial stem cells, which seriously impairs sight. Autologous limbal stem cell transplantation is effective in unilateral or partial bilateral disease but not applicable in total bilateral disease. An allogeneic source of transplantable cells for use in total bilateral disease can be obtained from culture of donated cadaveric corneal tissue. Read More

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http://doi.wiley.com/10.1002/sctm.18-0140
Publisher Site
http://dx.doi.org/10.1002/sctm.18-0140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431688PMC
April 2019
2 Reads

Mesenchymal Stem Cells from Chronic Pancreatitis Patients Show Comparable Potency Compared To Cells from Healthy Donors.

Stem Cells Transl Med 2019 Jan 24. Epub 2019 Jan 24.

Department of Surgery, Medical University of South Carolina, Charleston, South Carolina, USA.

Mesenchymal stem cells (MSCs) are proven to be beneficial in islet transplantation, suggesting a potential therapeutic role of them in total pancreatectomy with islet autotransplantation (TP-IAT) for chronic pancreatitis (CP) patients. We investigated whether MSCs derived from CP patients are suitable for use in autologous cell therapy. MSCs from healthy donors (H-MSCs) and CP patients (CP-MSCs) were studied for phenotype, colony formation potential, multilineage differentiation ability, proliferation, senescence, secretory characters, and immunosuppressive functions. Read More

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http://dx.doi.org/10.1002/sctm.18-0093DOI Listing
January 2019
5 Reads

Challenges Toward the Identification of Predictive Markers for Human Mesenchymal Stromal Cells Chondrogenic Potential.

Stem Cells Transl Med 2019 Feb;8(2):194-204

Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.

Human bone marrow derived mesenchymal stromal cells (BMSCs) represent a putative cell source candidate for tissue engineering-based strategies to repair cartilage and bone. However, traditional isolation of BMSCs by adhesion to plastic leads to very heterogeneous cell populations, accounting for high variability of chondrogenic differentiation outcome, both across donors and across clonally derived strains. Identification of putative surface markers able to select BMSC subpopulations with higher chondrogenic capacity (CC) and reduced variance in chondrogenic differentiation could aid the development of BMSC-based cartilage and bone regeneration approaches. Read More

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http://dx.doi.org/10.1002/sctm.18-0147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344903PMC
February 2019
1 Read

Chondroitin Sulfate Glycosaminoglycan Scaffolds for Cell and Recombinant Protein-Based Bone Regeneration.

Stem Cells Transl Med 2019 Jan 21. Epub 2019 Jan 21.

Regenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.

Bone morphogenetic protein 2 (BMP-2)-loaded collagen sponges remain the clinical standard for treatment of large bone defects when there is insufficient autograft, despite associated complications. Recent efforts to negate comorbidities have included biomaterials and gene therapy approaches to extend the duration of BMP-2 release and activity. In this study, we compared the collagen sponge clinical standard to chondroitin sulfate glycosaminoglycan (CS-GAG) scaffolds as a delivery vehicle for recombinant human BMP-2 (rhBMP-2) and rhBMP-2 expression via human BMP-2 gene inserted into mesenchymal stem cells (BMP-2 MSC). Read More

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http://dx.doi.org/10.1002/sctm.18-0141DOI Listing
January 2019
2 Reads

Corneal Wound Healing Effects of Mesenchymal Stem Cell Secretome Delivered Within a Viscoelastic Gel Carrier.

Stem Cells Transl Med 2019 Jan 15. Epub 2019 Jan 15.

Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, California, USA.

Severe corneal injuries often result in permanent vision loss and remain a clinical challenge. Human bone marrow derived mesenchymal stem cells (MSCs) and their secreted factors (secretome) have been studied for their antiscarring, anti-inflammatory, and antiangiogeneic properties. We aimed to deliver lyophilized MSC secretome (MSC-S) within a viscoelastic gel composed of hyaluronic acid (HA) and chondroitin sulfate (CS) as a way to enhance corneal re-epithelialization and reduce complications after mechanical and chemical injuries of the cornea. Read More

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http://doi.wiley.com/10.1002/sctm.18-0178
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http://dx.doi.org/10.1002/sctm.18-0178DOI Listing
January 2019
12 Reads

A Preview of Selected Articles.

Stem Cells Transl Med 2019 Feb 11;8(2):107-109. Epub 2019 Jan 11.

Centro de Investigación Príncipe Felipe, Valencia, Spain.

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http://dx.doi.org/10.1002/sctm.18-0296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344900PMC
February 2019
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Salidroside-Pretreated Mesenchymal Stem Cells Enhance Diabetic Wound Healing by Promoting Paracrine Function and Survival of Mesenchymal Stem Cells Under Hyperglycemia.

Stem Cells Transl Med 2019 Apr 9;8(4):404-414. Epub 2019 Jan 9.

The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, People's Republic of China.

Systemic abnormalities cause several complications in diabetes patients. Impaired wound healing is a serious complication that leads to severe foot ulcer and amputation. Mesenchymal stem cells (MSCs) have been considered a promising strategy for promoting wound healing due to their paracrine function. Read More

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http://doi.wiley.com/10.1002/sctm.18-0143
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http://dx.doi.org/10.1002/sctm.18-0143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431607PMC
April 2019
5 Reads

White Matter Tract Changes Associated with Clinical Improvement in an Open-Label Trial Assessing Autologous Umbilical Cord Blood for Treatment of Young Children with Autism.

Stem Cells Transl Med 2019 Feb 8;8(2):138-147. Epub 2019 Jan 8.

Duke Center for Autism and Brain Development, Department of Psychiatry and Behavioral Sciences, Duke University School of Medicine, Durham, North Carolina, USA.

Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder characterized by social communication deficits and the presence of restricted interests and repetitive behaviors. We have previously reported significant improvements in behavior, including increased social functioning, improved communication abilities, and decreased clinical symptoms in children with ASD, following treatment with a single infusion of autologous cord blood in a phase I open-label trial. In the current study, we aimed to understand whether these improvements were associated with concurrent changes in brain structural connectivity. Read More

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http://dx.doi.org/10.1002/sctm.18-0251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344899PMC
February 2019
3 Reads

Perfluorocarbon Labeling of Human Glial-Restricted Progenitors for F Magnetic Resonance Imaging.

Stem Cells Transl Med 2019 Apr 7;8(4):355-365. Epub 2019 Jan 7.

Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

One of the fundamental limitations in assessing potential efficacy in Central Nervous System (CNS) transplantation of stem cells is the capacity for monitoring cell survival and migration noninvasively and longitudinally. Human glial-restricted progenitor (hGRP) cells (Q-Cells) have been investigated for their utility in providing neuroprotection following transplantation into models of amyotrophic lateral sclerosis (ALS) and have been granted a Food and Drug Administration (FDA) Investigational New Drug (IND) for intraspinal transplantation in ALS patients. Furthermore, clinical development of these cells for therapeutic use will rely on the ability to track the cells using noninvasive imaging methodologies as well as the verification that the transplanted GRPs have disease-relevant activity. Read More

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http://doi.wiley.com/10.1002/sctm.18-0094
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http://dx.doi.org/10.1002/sctm.18-0094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431733PMC
April 2019
3 Reads

Concise Review: Therapeutic Potential of the Mesenchymal Stem Cell Derived Secretome and Extracellular Vesicles for Radiation-Induced Lung Injury: Progress and Hypotheses.

Stem Cells Transl Med 2019 Apr 7;8(4):344-354. Epub 2019 Jan 7.

Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas, USA.

Radiation-induced lung injury (RILI) is a common complication in radiotherapy of thoracic tumors and limits the therapeutic dose of radiation that can be given to effectively control tumors. RILI develops through a complex pathological process, resulting in induction and activation of various cytokines, infiltration by inflammatory cells, cytokine-induced activation of fibroblasts, and subsequent tissue remodeling by activated fibroblasts, ultimately leading to impaired lung function and respiratory failure. Increasing evidence shows that mesenchymal stem cells (MSCs) may play a main role in modulating inflammation and immune responses, promoting survival and repair of damaged resident cells and enhancing regeneration of damaged tissue through soluble paracrine factors and therapeutic extracellular vesicles. Read More

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http://doi.wiley.com/10.1002/sctm.18-0038
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http://dx.doi.org/10.1002/sctm.18-0038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431606PMC
April 2019
14 Reads
3.596 Impact Factor

Umbilical Cord-Derived Mesenchymal Stromal Cells (MSCs) for Knee Osteoarthritis: Repeated MSC Dosing Is Superior to a Single MSC Dose and to Hyaluronic Acid in a Controlled Randomized Phase I/II Trial.

Stem Cells Transl Med 2019 Mar 28;8(3):215-224. Epub 2018 Dec 28.

Cells for Cells & Consorcio Regenero, Santiago, Chile.

Knee osteoarthritis (OA) is a leading cause of pain and disability. Although conventional treatments show modest benefits, pilot and phase I/II trials with bone marrow (BM) and adipose-derived (AD) mesenchymal stromal cells (MSCs) point to the feasibility, safety, and occurrence of clinical and structural improvement in focal or diffuse disease. This study aimed to assess the safety and efficacy of the intra-articular injection of single or repeated umbilical cord-derived (UC) MSCs in knee OA. Read More

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http://dx.doi.org/10.1002/sctm.18-0053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392367PMC
March 2019
6 Reads