1,709 results match your criteria Stem cells international[Journal]


Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via Repression in Zebrafish.

Stem Cells Int 2019 12;2019:8451282. Epub 2019 Mar 12.

Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, USA.

Liver regeneration after most forms of injury is mediated through the proliferation of hepatocytes. However, when hepatocyte proliferation is impaired, such as during chronic liver disease, liver progenitor cells (LPCs) arising from the biliary epithelial cell (BEC) compartment can give rise to hepatocytes to mediate hepatic repair. Promotion of LPC-to-hepatocyte differentiation in patients with chronic liver disease could serve as a potentially new therapeutic option, but first requires the identification of the molecular mechanisms driving this process. Read More

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http://dx.doi.org/10.1155/2019/8451282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434270PMC

Identification and Characterization of the Upstream Regulatory Region in .

Stem Cells Int 2019 12;2019:2130973. Epub 2019 Mar 12.

Department of Agricultural Biotechnology, Animal Biotechnology Major, Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.

plays pivotal roles in maintaining pluripotency during early mammalian embryonic development and in embryonic stem cells. It is essential to establish a reporter system based on the promoter region to study pluripotency. However, there is still a lack of information about the porcine upstream reporter system. Read More

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https://www.hindawi.com/journals/sci/2019/2130973/
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http://dx.doi.org/10.1155/2019/2130973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434273PMC
March 2019
1 Read

Current Status and Perspectives of Human Mesenchymal Stem Cell Therapy.

Stem Cells Int 2019 7;2019:4762634. Epub 2019 Mar 7.

Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1155/2019/4762634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431493PMC

Cord Blood Banking: Antenatal Care Provider's Roles and Responsibilities.

Stem Cells Int 2019 7;2019:3598404. Epub 2019 Mar 7.

Manipal Academy of Higher Education, Department of Obstetrics & Gynecology, Kasturba Medical College, Manipal 576104, India.

Background: Umbilical Cord Blood (UCB) banking done either for private storage or for donation to public cord blood banks involves active participation of obstetricians. Counseling the expectant parents, providing them with unbiased and balanced information, and collecting the UCB with diligence confer a lot of social as well as moral responsibility upon obstetricians. This makes it even more important that the obstetricians in current practice stay well-informed and updated with UCB collection and its storage guidelines. Read More

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http://dx.doi.org/10.1155/2019/3598404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431514PMC
March 2019
1 Read

Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker.

Stem Cells Int 2019 10;2019:3526409. Epub 2019 Mar 10.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, 56 Lingyuanxi Road, Guangzhou 510055, China.

Stem cells from human exfoliated deciduous teeth (SHED) are a favourable source for tissue engineering, for its great proliferative capacity and the ease of collection. However, the transplantation of stem cells and the study of stem cell-based tissue engineering require massive stem cells. After long-term expansion, stem cells face many challenges, including limited lifespan, senescence, and loss of stemness. Read More

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http://dx.doi.org/10.1155/2019/3526409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431526PMC

The Genetic Landscape of Human Glioblastoma and Matched Primary Cancer Stem Cells Reveals Intratumour Similarity and Intertumour Heterogeneity.

Stem Cells Int 2019 7;2019:2617030. Epub 2019 Mar 7.

Laboratory of Experimental Neurosurgery and Cell Therapy, Neurosurgery Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Italy.

Glioblastoma (GBM) is the most malignant human brain tumour, characterized by rapid progression, invasion, intense angiogenesis, high genomic instability, and resistance to therapies. Despite countless experimental researches for new therapeutic strategies and promising clinical trials, the prognosis remains extremely poor, with a mean survival of less than 14 months. GBM aggressive behaviour is due to a subpopulation of tumourigenic stem-like cells, GBM stem cells (GSCs), which hierarchically drive onset, proliferation, and tumour recurrence. Read More

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http://dx.doi.org/10.1155/2019/2617030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431486PMC

Corrigendum to "Comparison of the Proliferation and Differentiation Potential of Human Urine-, Placenta Decidua Basalis-, and Bone Marrow-Derived Stem Cells".

Stem Cells Int 2019 10;2019:1651506. Epub 2019 Mar 10.

Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610000, China.

[This corrects the article DOI: 10.1155/2018/7131532.]. Read More

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http://dx.doi.org/10.1155/2019/1651506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431511PMC

Mesenchymal Stem Cells: Characteristics, Function, and Application.

Stem Cells Int 2019 6;2019:8106818. Epub 2019 Mar 6.

Division of Biomedical Sciences, University of Southern California, Los Angeles, CA, USA.

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http://dx.doi.org/10.1155/2019/8106818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431372PMC

Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients.

Stem Cells Int 2019 6;2019:7532657. Epub 2019 Mar 6.

Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.

Induced pluripotent stem cells (iPSCs) from type 1 long QT (LQT1) patients can differentiate into cardiomyocytes (CMs) including ventricular cells to recapitulate the disease phenotype. Although optical recordings using membrane potential dyes to monitor action potentials (APs) were reported, no study has investigated the disease phenotypes of cardiac channelopathy in association with the cardiac subtype at the single-cell level. We induced iPSC-CMs from three control and three LQT1 patients. Read More

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http://dx.doi.org/10.1155/2019/7532657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431403PMC
March 2019
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Experimental Strategies of Mesenchymal Stem Cell Propagation: Adverse Events and Potential Risk of Functional Changes.

Stem Cells Int 2019 6;2019:7012692. Epub 2019 Mar 6.

NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.

Mesenchymal stem cells (MSCs) are attractive candidates for cell-based tissue repair approaches. Hundreds of clinical trials using MSCs have been completed and many others are still being investigated. For most therapeutic applications, MSC propagation is often required. Read More

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https://www.hindawi.com/journals/sci/2019/7012692/
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http://dx.doi.org/10.1155/2019/7012692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431404PMC
March 2019
2 Reads

Different Responses to Drug Safety Screening Targets between Human Neonatal and Infantile Heart Tissue and Cardiac Bodies Derived from Human-Induced Pluripotent Stem Cells.

Stem Cells Int 2019 6;2019:6096294. Epub 2019 Mar 6.

Department of Paediatric Cardiology, Heart Centre Cologne, University Hospital of Cologne, Kerpener Str. 52, 50924 Cologne, Germany.

Aims: Induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) have become a promising tool in cardiovascular safety pharmacology. Immaturity of iPS-CMs remains an ongoing concern. We compared electrophysiological and contractile features of cardiac bodies (hiPS-CBs) derived from human-induced pluripotent stem cells and human neonatal and infantile myocardial slices relevant for drug screening. Read More

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http://dx.doi.org/10.1155/2019/6096294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431377PMC
March 2019
1 Read

Corrigendum to "Impact and Challenges of Mesenchymal Stem Cells in Medicine: An Overview of the Current Knowledge".

Stem Cells Int 2019 10;2019:5493654. Epub 2019 Mar 10.

Institute for Macromolecular Chemistry, Faculty of Chemistry and Pharmacy, BIOSS-Centre for Biological Signalling Studies, University of Freiburg, Hermann Staudinger Haus, Stefan-Meier Strasse 31, Freiburg D-79104, Germany.

[This corrects the article DOI: 10.1155/2018/5023925.]. Read More

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http://dx.doi.org/10.1155/2019/5493654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431428PMC

Effect of Chitosan Nanoparticle-Loaded on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage.

Stem Cells Int 2019 26;2019:5142518. Epub 2019 Feb 26.

Tissue Engineering Group (TEG), National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.

We have determined the protective effects of (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) . Gas chromatography-mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Read More

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http://dx.doi.org/10.1155/2019/5142518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431441PMC
February 2019

Morphological and Molecular Analysis of In Vitro Tubular Structures from Bovine Yolk Sac-Derived MSCs.

Stem Cells Int 2019 6;2019:5073745. Epub 2019 Mar 6.

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.

The yolk sac is an extraembryonic membrane, of saccular form, connected to the ventral region of the embryo. It is the main source of nutrition for the embryo during the period when the placenta is not fully formed. The aim of this study was to generate tubular structures using mesenchymal stem cells from the bovine yolk sac (bYS-MSCs) and determine if these structures can be a model for in vitro vasculogenesis. Read More

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http://dx.doi.org/10.1155/2019/5073745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431375PMC

One-Carbon Metabolism Links Nutrition Intake to Embryonic Development via Epigenetic Mechanisms.

Stem Cells Int 2019 10;2019:3894101. Epub 2019 Mar 10.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.

Beyond energy production, nutrient metabolism plays a crucial role in stem cell lineage determination. Changes in metabolism based on nutrient availability and dietary habits impact stem cell identity. Evidence suggests a strong link between metabolism and epigenetic mechanisms occurring during embryonic development and later life of offspring. Read More

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http://dx.doi.org/10.1155/2019/3894101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431457PMC

Mesenchymal Stem Cells: A Potential Therapeutic Approach for Amyotrophic Lateral Sclerosis?

Stem Cells Int 2019 10;2019:3675627. Epub 2019 Mar 10.

IRCCS Centro Neurolesi "Bonino Pulejo", 98124 Messina, Italy.

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the degeneration of both upper and lower motor neurons. Patients show both motor and extra-motor symptoms. A cure is not available at this time, and the disease leads to death within 3-5 years, mainly due to respiratory failure. Read More

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http://dx.doi.org/10.1155/2019/3675627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431432PMC
March 2019
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Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic -Cells.

Stem Cells Int 2019 10;2019:1395301. Epub 2019 Mar 10.

The School of Life Sciences and Centre for Health Technologies, Faculty of Science, University of Technology Sydney, Sydney, Australia.

Combinatorial gene and cell therapy as a means of generating surrogate -cells has been investigated for the treatment of type 1 diabetes (T1D) for a number of years with varying success. One of the limitations of current cell therapies for T1D is the inability to generate sufficient quantities of functional transplantable insulin-producing cells. Due to their impressive immunomodulatory properties, in addition to their ease of expansion and genetic modification , mesenchymal stem cells (MSCs) are an attractive alternative source of adult stem cells for regenerative medicine. Read More

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http://dx.doi.org/10.1155/2019/1395301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431458PMC

Corrigendum to "Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells".

Stem Cells Int 2019 5;2019:9071046. Epub 2019 Mar 5.

Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea.

[This corrects the article DOI: 10.1155/2015/106540.]. Read More

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http://dx.doi.org/10.1155/2019/9071046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425339PMC

Stem Cells in Cancer Progression and Therapy.

Stem Cells Int 2019 5;2019:3507604. Epub 2019 Mar 5.

School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.

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http://dx.doi.org/10.1155/2019/3507604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425421PMC

Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction.

Stem Cells Int 2019 5;2019:2543171. Epub 2019 Mar 5.

Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.

The mechanisms of renal fibrogenesis after ureteral obstruction remain unclear. We tried to primarily expand mesenchymal stem cells from renal tissues and then investigated their role in fibrogenesis after ureteral obstruction. Unilateral ureteral obstruction was induced by ligating the left ureteral duct of adult C57BL/6 mice. Read More

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http://dx.doi.org/10.1155/2019/2543171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425387PMC

Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung.

Stem Cells Int 2019 27;2019:7043890. Epub 2019 Feb 27.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China.

Stem/progenitor cells have recently been demonstrated to play key roles in the maturation, injury repair, and regeneration of distinct organs or tissues. Porcine has spurred an increased interest in biomedical research models and xenotransplantation, owing to most of its organs share similarities in physiology, cellular composition and size to humans. Therefore, characterization of stem/progenitor cells in porcine organs or tissues may provide a novel avenue to better understand the biology and function of stem cells in humans. Read More

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http://dx.doi.org/10.1155/2019/7043890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415319PMC
February 2019
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NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells.

Stem Cells Int 2019 26;2019:5150634. Epub 2019 Feb 26.

Department of Internal Medicine II and Comprehensive Cancer Center Mainfranken, University Hospital of Würzburg, Oberdürrbacher Straße 6, 97080 Würzburg, Germany.

Skeletal development and remodeling of adult bone are critically controlled by activated NOTCH signaling in genetically modified mice. It is yet unclear whether NOTCH signaling is activated by mechanical strain sensed by bone cells. We found that expression of specific NOTCH target genes is induced after tibial mechanical loading in wild-type mice. Read More

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https://www.hindawi.com/journals/sci/2019/5150634/
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http://dx.doi.org/10.1155/2019/5150634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413410PMC
February 2019
2 Reads

Stem Cell-Derived Extracellular Vesicles as Immunomodulatory Therapeutics.

Stem Cells Int 2019 26;2019:5126156. Epub 2019 Feb 26.

Laboratory of Stem Cell Research, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21999, Republic of Korea.

Mesenchymal stem cells (MSCs) have been reported to possess regulatory functions on immune cells which make them alternative therapeutics for the treatment of inflammatory and autoimmune diseases. The interaction between MSCs and immune cells through paracrine factors might be crucial for these immunomodulatory effects of MSCs. Extracellular vesicles (EVs) are defined as bilayer membrane structures including exosomes and microvesicles which contain bioactive paracrine molecules affecting the characteristics of target cells. Read More

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http://dx.doi.org/10.1155/2019/5126156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413386PMC
February 2019

Pancreatic Progenitors and Organoids as a Prerequisite to Model Pancreatic Diseases and Cancer.

Stem Cells Int 2019 25;2019:9301382. Epub 2019 Feb 25.

Department of Internal Medicine 1, Ulm University Medical Centre, Albert-Einstein-Allee 23, 89081 Ulm, Germany.

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are characterized by their unique capacity to stepwise differentiate towards any particular cell type in an adult organism. Pluripotent stem cells provide a beneficial platform to model hereditary diseases and even cancer development. While the incidence of pancreatic diseases such as diabetes and pancreatitis is increasing, the understanding of the underlying pathogenesis of particular diseases remains limited. Read More

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https://www.hindawi.com/journals/sci/2019/9301382/
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http://dx.doi.org/10.1155/2019/9301382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410438PMC
February 2019
1 Read

Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation.

Stem Cells Int 2019 25;2019:4084351. Epub 2019 Feb 25.

Laboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology (CIBIO), Università degli Studi di Trento, Trento, Italy.

Epsins are part of the internalization machinery pivotal to control clathrin-mediated endocytosis. Here, we report that epsin family members are expressed in mouse embryonic stem cells (mESCs) and that epsin1/2 knockdown alters both mESC exits from pluripotency and their differentiation. Furthermore, we show that epsin1/2 knockdown compromises the correct polarization and division of mESC-derived neural progenitors and their conversion into expandable radial glia-like neural stem cells. Read More

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http://dx.doi.org/10.1155/2019/4084351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410434PMC
February 2019
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Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.

Stem Cells Int 2019 21;2019:9723025. Epub 2019 Feb 21.

Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

The present study was conducted to establish the amount of mechanical strain (uniaxial cyclic stretching) required to provide optimal tenogenic differentiation expression in human mesenchymal stromal cells (hMSCs) , in view of its potential application for tendon maintenance and regeneration. . In the present study, hMSCs were subjected to 1 Hz uniaxial cyclic stretching for 6, 24, 48, and 72 hours; and were compared to unstretched cells. Read More

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http://dx.doi.org/10.1155/2019/9723025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409073PMC
February 2019

Scalable Culture Strategies for the Expansion of Patient-Derived Cancer Stem Cell Lines.

Stem Cells Int 2019 6;2019:8347595. Epub 2019 Feb 6.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.

Cancer stem cells (CSCs) have recently raised great interest as a promising biological system for designing effective cancer therapies. The scarcity of CSCs and the consequent low numbers obtained from biopsies represent a major hurdle to the development of such strategies. It is therefore necessary to design robust scalable methods to enable efficient expansion of bona fide CSCs . Read More

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http://dx.doi.org/10.1155/2019/8347595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409046PMC
February 2019

The Biguanides Metformin and Buformin in Combination with 2-Deoxy-glucose or WZB-117 Inhibit the Viability of Highly Resistant Human Lung Cancer Cells.

Stem Cells Int 2019 21;2019:6254269. Epub 2019 Feb 21.

School of Pharmacy, Department of Pharmaceutical Sciences, Hampton University, VA 23668, USA.

The biguanides metformin (MET) and to a lesser extent buformin (BUF) have recently been shown to exert anticancer effects. In particular, MET targets cancer stem cells (CSCs) in a variety of cancer types but these compounds have not been extensively tested for combination therapy. In this study, we investigated the anticancer activity of MET and BUF alone or in combination with 2-deoxy-D-glucose (2-DG) and WZB-117 (WZB), which are a glycolysis and a GLUT-1 inhibitor, respectively, in H460 human lung cancer cells growing under three different culture conditions with varying degrees of stemness: (1) routine culture conditions (RCCs), (2) floating lung tumorspheres (LTSs) that are enriched for stem-like cancer cells, and (3) adherent cells under prolonged periods (8-12 days) of serum starvation (PPSS). Read More

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http://dx.doi.org/10.1155/2019/6254269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409035PMC
February 2019

Comparative Analysis of Biological Properties of Large-Scale Expanded Adult Neural Crest-Derived Stem Cells Isolated from Human Hair Follicle and Skin Dermis.

Stem Cells Int 2019 19;2019:9640790. Epub 2019 Feb 19.

Department of Genetic Diagnostics, State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, 67 Vyshgorodska Str., Kyiv 04114, Ukraine.

Introduction: The adult neural crest-derived stem cells (NCSCs) have significant perspectives for use in regenerative medicine. The most attractive sources for adult NCSC isolation are the hair follicles (HF) and skin dermis (SD) because of easy access and minimally invasive biopsy. The aim of this study was to compare the biological properties of HF- and SD-derived NCSCs after their large-scale expansion. Read More

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http://dx.doi.org/10.1155/2019/9640790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399535PMC
February 2019
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Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue.

Stem Cells Int 2019 19;2019:5901479. Epub 2019 Feb 19.

Cellular Neurobiology Laboratory, Department of Cerebrovascular Diseases, Fondazione IRCCS Neurological Institute C. Besta, Milan, Italy.

Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in a closed system device, has been successfully used in aesthetic medicine as well as in orthopedic and general surgery. Interestingly, in orthopedic diseases, this ready-to-use adipose tissue cell derivative seems to have a prolonged time efficacy even upon a single shot injection into osteoarthritic tissues. Here, we investigated the long-term survival and content of MSCs as well the anti-inflammatory activity of LP and its derived MFAT in vitro, with the aim to better understand a possible in vivo mechanism of action. Read More

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http://dx.doi.org/10.1155/2019/5901479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399530PMC
February 2019

Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish.

Stem Cells Int 2019 19;2019:3831213. Epub 2019 Feb 19.

Department of Internal Medicine I, Ulm University, Ulm, Germany.

Primary liver cancer (PLC) is the sixth most common tumour disease and one of the leading causes of cancer-related death worldwide. The two most common types of PLC are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). Diverse subgroups are described and a manifold number of gene mutations are known. Read More

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http://dx.doi.org/10.1155/2019/3831213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399527PMC
February 2019
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Characterization and Optimization of the Seeding Process of Adipose Stem Cells on the Polycaprolactone Scaffolds.

Stem Cells Int 2019 20;2019:1201927. Epub 2019 Feb 20.

Department of Regenerative Medicine, Maria Sklodowska Curie Institute-Oncology Center, Roentgena 5, 02-781 Warsaw, Poland.

The purpose of the current study was to evaluate the usefulness of adipose-derived stem cells (ASCs) for bone injury therapy. Lipoaspirates were collected from the abdomen regions of 17 healthy female donors (mean age 49 ± 6 years) using Coleman technique or Body-jet liposuction. In the present study, the primary objective was the characteristics of human ASCs. Read More

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http://dx.doi.org/10.1155/2019/1201927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402208PMC
February 2019

Insulin-Like Growth Factor Binding Protein-6 Promotes the Differentiation of Placental Mesenchymal Stem Cells into Skeletal Muscle Independent of Insulin-Like Growth Factor Receptor-1 and Insulin Receptor.

Stem Cells Int 2019 17;2019:9245938. Epub 2019 Feb 17.

Western University, Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, London, Ontario, Canada.

As mesenchymal stem cells (MSCs) are being investigated for regenerative therapies to be used in the clinic, delineating the roles of the IGF system in MSC growth and differentiation, , is vital in developing these cellular therapies to treat degenerative diseases. Muscle differentiation is a multistep process, starting with commitment to the muscle lineage and ending with the formation of multinucleated fibers. Insulin-like growth factor binding protein-6 (IGFBP-6), relative to other IGFBPs, has high affinity for IGF-2. Read More

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http://dx.doi.org/10.1155/2019/9245938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397983PMC
February 2019
1 Read

Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems.

Stem Cells Int 2019 18;2019:7486279. Epub 2019 Feb 18.

Department of Research, IRCCS-ISMETT, Palermo, Italy.

The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. Read More

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http://dx.doi.org/10.1155/2019/7486279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397962PMC
February 2019

Obesity-Induced Methylation of Osteopontin Contributes to Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells.

Stem Cells Int 2019 17;2019:1238153. Epub 2019 Feb 17.

Shanghai Key Laboratory for Molecular Imaging, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.

Obesity is a major risk factor for many chronic diseases, including diabetes, fatty livers, and cancer. Expansion of the adipose mass has been shown to be related to adipogenic differentiation of adipose-derived mesenchymal stem cells (ASCs). However, the underlying mechanism of this effect has yet to be elucidated. Read More

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http://dx.doi.org/10.1155/2019/1238153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398038PMC
February 2019
2.806 Impact Factor

Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.

Stem Cells Int 2019 13;2019:8203950. Epub 2019 Feb 13.

Unità di Biologia Vascolare e Medicina Rigenerativa, Centro Cardiologico Monzino IRCCS, via Carlo Parea 4, Milan, Italy.

The cellular response to the extracellular matrix (ECM) microenvironment mediated by integrin adhesion is of fundamental importance, in both developmental and pathological processes. In particular, mechanotransduction is of growing importance in groundbreaking cellular models such as induced pluripotent stem cells (iPSC), since this process may strongly influence cell fate and, thus, augment the precision of differentiation into specific cell types, e.g. Read More

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https://www.hindawi.com/journals/sci/2019/8203950/
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http://dx.doi.org/10.1155/2019/8203950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393933PMC
February 2019
3 Reads

Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth into Retinal Photoreceptor-Like Cells and Their Sustainability .

Stem Cells Int 2019 14;2019:2562981. Epub 2019 Feb 14.

Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, and Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.

Retinal degeneration is characterized by the progressive loss of photoreceptors, and stem cell therapy has become a promising strategy. Many studies have reported that mesenchymal stem cell transplantation can sustain retinal structure and prolong retinal functions based on two mechanisms. One is cell replacement, and the other is the paracrine action of stem cells. Read More

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http://dx.doi.org/10.1155/2019/2562981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393909PMC
February 2019
2.806 Impact Factor

Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process.

Stem Cells Int 2019 11;2019:2340725. Epub 2019 Feb 11.

Evangelical Faculty of Paraná, Brazil.

Burns are lesions in which the thermal energy of the causative agent transfers heat to the surface of the body, causing superficial or deep damage to the skin with protein denaturation in cells and biochemical maladjustments, which delay and disrupt the cicatricial process, increasing the chances of functional and aesthetic sequelae. This study evaluates the influence of adipose tissue-derived stem cells (ADSCs) on burn healing in terms of the size of the cicatricial space and quantified measures of collagen deposition, inflammatory infiltrate, blood vessels, and lymphatic vessels. Initially, intra-abdominal adipose tissue was resected from a single donor Wistar rat that was not part of any of the subsequent groups to obtain ADSCs by isolation and cell culture. Read More

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https://www.hindawi.com/journals/sci/2019/2340725/
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http://dx.doi.org/10.1155/2019/2340725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388323PMC
February 2019
10 Reads

2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro.

Stem Cells Int 2019 7;2019:7246479. Epub 2019 Feb 7.

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan-Jiang West Road, Guangzhou 510120, China.

Extracellular vesicles (Evs) contain diverse functional proteins, mRNAs, miRNAs, and DNA fragments, are secreted by various types of cells, and play important roles in cellular communication. Here, we show for the first time that plasma Evs inhibited the osteogenic differentiation of mesenchymal stromal cells (MSCs) in vitro and the level of inhibition was positively correlated with the plasma Evs concentration. Plasma Evs downregulated the expression of markers such as osteocalcin (OCN), Runt-related transcription factor 2 (Runx2), and Osterix at mRNA levels required for osteogenic differentiation and reduced pSmad1/5/8 levels in MSCs. Read More

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http://dx.doi.org/10.1155/2019/7246479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383392PMC
February 2019

Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration .

Stem Cells Int 2019 7;2019:5130152. Epub 2019 Feb 7.

Institute of Orthopaedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, 28 Fuxing Road, Haidian District, Beijing 100853, China.

Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvironment was constructed by primary articular cartilage cells (pACs) and acellular cartilage extracellular matrix- (ACECM-) oriented scaffold cocultured with human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJMSCs) The coculture groups showed more affluent cartilage special matrix ingredients including collagen II and aggrecan based on the results of histological staining and western blotting and cut down as many pACs as possible. The RT-PCR and cell viability experiments also demonstrated that hWJMSCs were successfully induced to differentiate into chondrocytes when cultured in the simulated cartilage microenvironment, as confirmed by the significant upregulation of collagen II and aggrecan, while the cell proliferation activity of pACs was significantly improved by cell-cell interactions. Read More

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http://dx.doi.org/10.1155/2019/5130152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383394PMC
February 2019
2 Reads

Mitochondrial Role in Stemness and Differentiation of Hematopoietic Stem Cells.

Stem Cells Int 2019 6;2019:4067162. Epub 2019 Feb 6.

Division of Hematology/Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Quiescent and self-renewing hematopoietic stem cells (HSCs) rely on glycolysis rather than on mitochondrial oxidative phosphorylation (OxPHOS) for energy production. HSC reliance on glycolysis is considered an adaptation to the hypoxic environment of the bone marrow (BM) and reflects the low energetic demands of HSCs. Metabolic rewiring from glycolysis to mitochondrial-based energy generation accompanies HSC differentiation and lineage commitment. Read More

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http://dx.doi.org/10.1155/2019/4067162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381553PMC
February 2019

CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury.

Stem Cells Int 2019 5;2019:2457082. Epub 2019 Feb 5.

Center of Basic Medical Sciences of Navy General Hospital, Beijing 100048, China.

Less quantity of transplanted mesenchymal stem cells (MSCs) influences the therapeutic effects on radiation-induced lung injury (RILI). Previous studies have demonstrated that MSCs overexpressing Chemokine (C-X-C motif) receptor 4 (CXCR4) could increase the quantity of transplanted cells to local tissues. In the present study, we conducted overexpressing CXCR4 human umbilical cord mesenchymal stem cell (HUMSC) therapy for RILI. Read More

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https://www.hindawi.com/journals/sci/2019/2457082/
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http://dx.doi.org/10.1155/2019/2457082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379846PMC
February 2019
6 Reads

Characteristics of Intestinal Microecology during Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury.

Stem Cells Int 2019 5;2019:2403793. Epub 2019 Feb 5.

State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Rd., Hangzhou City 310003, China.

Background: The mechanisms of mesenchymal stem cell (MSC) transplantation to protect against acute liver injury have been well studied within the liver. However, the associated changes in the intestinal microbiota during this process are poorly understood.

Methods: In this study, compact bone-derived MSCs were injected into mice after carbon tetrachloride (CCl) administration. Read More

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http://dx.doi.org/10.1155/2019/2403793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379839PMC
February 2019
1 Read

Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells.

Stem Cells Int 2019 4;2019:4254759. Epub 2019 Feb 4.

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China.

Sweat glands developed from the embryonic epidermis. To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed. Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) using Transwell insert for evaluating the effects of soluble morphogens on sweat gland morphogenesis in vitro. Read More

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http://dx.doi.org/10.1155/2019/4254759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378793PMC
February 2019

Combination Treatments of Plasma Exchange and Umbilical Cord-Derived Mesenchymal Stem Cell Transplantation for Patients with Hepatitis B Virus-Related Acute-on-Chronic Liver Failure: A Clinical Trial in China.

Stem Cells Int 2019 4;2019:4130757. Epub 2019 Feb 4.

Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630 Guangdong, China.

Background: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a common type of liver failure with a high mortality. This study aimed at investigating the safety and efficacy of the combination treatment of plasma exchange (PE) and umbilical cord-derived mesenchymal stem cell (UC-MSCs) transplantation for HBV-ACLF patients.

Methods: A total of 110 HBV-ACLF patients treated in our hospital from January 2012 to September 2017 were enrolled into this trial and divided into the control group ( = 30), UC-MSC group ( = 30), PE group ( = 30), and UC-MSC + PE group ( = 20) based on their treatments. Read More

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http://dx.doi.org/10.1155/2019/4130757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378797PMC
February 2019
1 Read

The Role of RNA Polymerase II Contiguity and Long-Range Interactions in the Regulation of Gene Expression in Human Pluripotent Stem Cells.

Stem Cells Int 2019 3;2019:1375807. Epub 2019 Feb 3.

Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Botanicka 68a, Brno 60200, Czech Republic.

The eukaryotic nucleus is a highly complex structure that carries out multiple functions primarily needed for gene expression, and among them, transcription seems to be the most fundamental. Diverse approaches have demonstrated that transcription takes place at discrete sites known as transcription factories, wherein RNA polymerase II (RNAP II) is attached to the factory and immobilized while transcribing DNA. It has been proposed that transcription factories promote chromatin loop formation, creating long-range interactions in which relatively distant genes can be transcribed simultaneously. Read More

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http://dx.doi.org/10.1155/2019/1375807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378007PMC
February 2019
2 Reads

Mesenchymal Stem Cells Coated by the Extracellular Matrix Promote Wound Healing in Diabetic Rats.

Stem Cells Int 2019 28;2019:9564869. Epub 2019 Jan 28.

Department of Endocrinology and Metabolism, Third Xiangya Hospital of Central South University, China.

Objective: To investigate the effects of mesenchymal stem cells (MSCs) coated by the extracellular matrix (ECM) on wound healing in diabetic rats.

Methods: Mesenchymal stem cells were cocultured with ECM. Cell viabilities were evaluated using MTT assay. Read More

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http://dx.doi.org/10.1155/2019/9564869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369500PMC
January 2019
3 Reads

Wnt-GSK3/-Catenin Regulates the Differentiation of Dental Pulp Stem Cells into Bladder Smooth Muscle Cells.

Stem Cells Int 2019 28;2019:8907570. Epub 2019 Jan 28.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, No. 145 Western Changle Road, Xi'an, Shaanxi 710032, China.

Smooth muscle cell- (SMC-) based tissue engineering provides a promising therapeutic strategy for SMC-related disorders. It has been demonstrated that human dental pulp stem cells (DPSCs) possess the potential to differentiate into mature bladder SMCs by induction with condition medium (CM) from bladder SMC culture, in combination with the transforming growth factor-1 (TGF-1). However, the molecular mechanism of SMC differentiation from DPSCs has not been fully uncovered. Read More

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http://dx.doi.org/10.1155/2019/8907570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369468PMC
January 2019
3 Reads

Intestinal Epithelial Organoids as Tools to Study Epigenetics in Gut Health and Disease.

Stem Cells Int 2019 27;2019:7242415. Epub 2019 Jan 27.

Department of Paediatrics, University of Cambridge, Cambridge, UK.

The intestinal epithelium forms the inner layer of the human intestine and serves a wide range of diverse functions. Its constant exposure to a vast amount of complex microbiota highlights the critical interface that this single-cell layer forms between the host and our environment. Importantly, the well-documented contribution of environmental factors towards the functional development of the human intestinal epithelium directly implies epigenetic mechanisms in orchestrating this complex interplay. Read More

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http://dx.doi.org/10.1155/2019/7242415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369455PMC
January 2019
1 Read

Cell Therapy for Retinal Dystrophies: From Cell Suspension Formulation to Complex Retinal Tissue Bioengineering.

Stem Cells Int 2019 23;2019:4568979. Epub 2019 Jan 23.

INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France.

Retinal degeneration is an irreversible phenomenon caused by various disease conditions including age-related macular degeneration (AMD) and retinitis pigmentosa (RP). During the course of these diseases, photoreceptors (PRs) are susceptible to degeneration due to their malfunctions or to a primary dysfunction of the retinal pigment epithelium (RPE). Once lost, these cells could not be endogenously regenerated in humans, and cell therapy to replace the lost cells is one of the promising strategies to recover vision. Read More

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http://dx.doi.org/10.1155/2019/4568979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364130PMC
January 2019