513,631 results match your criteria Stem cells Dayton Ohio[Journal]


Disordered APC/C-mediated cell cycle progression and IGF1/PI3K/AKT signalling are the potential basis of Sertoli cell-only syndrome.

Andrologia 2019 Apr 17:e13288. Epub 2019 Apr 17.

Institute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.

The cause of Sertoli cell-only syndrome (SCOS), a condition in which only Sertoli cells line the seminiferous tubules in the testis, is unknown. Three microarray data sets were downloaded from public databases and were used to compare SCOS and control group. A total of 291 genes differentially expressed (Log |FC| ≥ 1 and adjusted p value < 0. Read More

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http://dx.doi.org/10.1111/and.13288DOI Listing

Rare case of an oligospermic male with 46,XX/46,XY tetragametic chimerism.

Andrologia 2019 Apr 17:e13290. Epub 2019 Apr 17.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Chimerism, a rare human disorder, is assumed to be the result of an amalgamation of two separate zygotes in a single embryo. Studies have shown that the phenotypic spectrum of chimerism is variable and there is no definite genotype-phenotype correlation in patients with chimerism, therefore a majority of cases might remain undiagnosed. This study aims to investigate the possible mechanism of the chimerism in a 46,XX/46,XY infertile and phenotypically normal male, with 46,XX blood karyotype and normal spermatogenesis. Read More

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http://dx.doi.org/10.1111/and.13290DOI Listing

Importance of point defect reactions for the atomic-scale roughness of III-V nanowire sidewalls.

Nanotechnology 2019 Apr 17. Epub 2019 Apr 17.

Departement Physique, Institut Superieur d'Electronique du Nord, 41 Boulevard Vauban, F-59046 Lille Cedex, Lille, FRANCE.

The surface morphology of III-V semiconductor nanowires protected by an arsenic cap and subsequently evaporated in ultrahigh vacuum is investigated with scanning tunneling microscopy and scanning transmission electron microscopy. We show that the changes of the surface morphology as a function of the nanowire composition and the nature of the seed particles are intimately related to the formation and reaction of surface point defects. Langmuir evaporation close to the congruent evaporation temperature causes the formation of vacancies which nucleate and form vacancy islands on {110} sidewalls of self-catalysed InAs nanowires. Read More

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http://dx.doi.org/10.1088/1361-6528/ab1a4eDOI Listing

Gradients of Be-dopant concentration in self-catalyzed GaAs nanowires.

Nanotechnology 2019 Apr 17. Epub 2019 Apr 17.

Optoelectronics Research Centre, Physics Unit, Tampere University, Tampere, FINLAND.

Effective and controllable doping is instrumental for enabling the use of III-V semiconductor nanowires in practical electronics and optoelectronics applications. To this end, dopants are incorporated during self-catalyzed growth via vapour-liquid-solid mechanism through the catalyst droplet or by vapour-solid mechanism of the sidewall growth. The interplay of these mechanisms together with the competition between axial elongation and radial growth of nanowires can result in dopant concentration gradients along the nanowire axis. Read More

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http://dx.doi.org/10.1088/1361-6528/ab1a97DOI Listing

Cytotoxic effect of aqueous ethanolic extract of Luffa cylindrica leaves on cancer stem cells CD44/24 in breast cancer patients with various molecular sub-types using tissue samples in vitro.

J Ethnopharmacol 2019 Apr 14:111877. Epub 2019 Apr 14.

Medical Research Department, National Institute of Occupational Safety and Health, Egypt. Electronic address:

Ethnopharmacological Relevance: Luffa cylindrica (L.) M.Roem is a climbing plant its parts have been used as traditional medicine for the treatment of different types of diseases including diarrhea, inflammation, cancer and viral infections. Read More

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http://dx.doi.org/10.1016/j.jep.2019.111877DOI Listing

Clinical, immunologic, and molecular spectrum of patients with LPS-responsive beige-like anchor protein (LRBA) deficiency: a systematic review.

J Allergy Clin Immunol Pract 2019 Apr 14. Epub 2019 Apr 14.

Non-communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran. Electronic address:

Background: LPS-responsive beige-like anchor protein (LRBA) deficiency is a primary immunodeficiency and immune dysregulation syndrome caused by biallelic mutations in the LRBA gene. These mutations usually abrogate the protein expression of LRBA, leading to a broad spectrum of clinical phenotypes including autoimmunity, chronic diarrhea, hypogammaglobulinemia and recurrent infections.

Objective: Our aim was to systematically collect all studies reporting on the clinical manifestations, molecular and laboratory findings, and management of patients with LRBA deficiency. Read More

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http://dx.doi.org/10.1016/j.jaip.2019.04.011DOI Listing

Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells.

Cell Rep 2019 Apr;27(3):971-986.e9

Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada; Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. Electronic address:

Glioblastoma therapies have remained elusive due to limitations in understanding mechanisms of growth and survival of the tumorigenic population. Using CRISPR-Cas9 approaches in patient-derived GBM stem cells (GSCs) to interrogate function of the coding genome, we identify actionable pathways responsible for growth, which reveal the gene-essential circuitry of GBM stemness and proliferation. In particular, we characterize members of the SOX transcription factor family, SOCS3, USP8, and DOT1L, and protein ufmylation as important for GSC growth. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22111247193036
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http://dx.doi.org/10.1016/j.celrep.2019.03.047DOI Listing
April 2019
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Functional Network Analysis Reveals the Relevance of SKIIP in the Regulation of Alternative Splicing by p38 SAPK.

Cell Rep 2019 Apr;27(3):847-859.e6

Cell Signaling Research Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address:

Alternative splicing is a prevalent mechanism of gene regulation that is modulated in response to a wide range of extracellular stimuli. Stress-activated protein kinases (SAPKs) play a key role in controlling several steps of mRNA biogenesis. Here, we show that osmostress has an impact on the regulation of alternative splicing (AS), which is partly mediated through the action of p38 SAPK. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.060DOI Listing

lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation.

Cell Rep 2019 Apr;27(3):719-729.e6

Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge CB2 0RE, UK; Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA; New York Genome Center, New York, NY 10013, USA. Electronic address:

Long non-coding RNAs (lncRNAs) show patterns of tissue- and cell type-specific expression that are very similar to those of protein coding genes and consequently have the potential to control stem and progenitor cell fate decisions along a differentiation trajectory. To understand the roles that lncRNAs may play in hematopoiesis, we selected a subset of mouse lncRNAs with potentially relevant expression patterns and refined our candidate list using evidence of conserved expression in human blood lineages. For each candidate, we assessed its possible role in hematopoietic differentiation in vivo using competitive transplantation. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.080DOI Listing

Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.

Cell Rep 2019 Apr;27(3):708-718.e10

Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address:

Studies in vertebrates have outlined conserved molecular control of definitive endoderm (END) development. However, recent work also shows that key molecular aspects of human END regulation differ even from rodents. Differentiation of human embryonic stem cells (ESCs) to END offers a tractable system to study the molecular basis of normal and defective human-specific END development. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.076DOI Listing

Lentiviral Gene Therapy Combined with Low-Dose Busulfan in Infants with SCID-X1.

N Engl J Med 2019 Apr;380(16):1525-1534

From the Departments of Bone Marrow Transplantation and Cellular Therapy (E.M., B.T., W.J., S.G.), Hematology (S.Z., Z.M., J.C., J.D., X.T., B.Y.R., M.J.W., B.P.S.), Therapeutics Production and Quality (T.L., M.M.M.), Immunology (H.A., B.Y.), Pharmaceutical Sciences (S.J.C.), Biostatistics (G.K., C.L.), and Infectious Diseases (G.M.), St. Jude Children's Research Hospital, Memphis, TN; the Allergy and Clinical Immunology Division, Hospital Nacional Edgardo Rebagliati Martins, Lima, Peru (J.C.A.B.); the Department of Pediatrics, Allergy-Immunology Division, Children's Hospital Los Angeles, Los Angeles (J.A.C.), and the Department of Pediatrics, Division of Pediatric Allergy-Immunology-Bone Marrow Transplantation, University of California, San Francisco (UCSF) Benioff Children's Hospital, San Francisco (J.R.L.-B., J.M.P., M.J.C.) - both in California; the Department of Pediatrics, Pediatric Allergy and Immunology, University of New Mexico, Albuquerque (E.D.); University of Oklahoma Health Sciences Center, Tulsa (J.T.L.); Departamento de Pediatria da Universidade de Taubaté, Conselho Nacional de Medicina, São Paulo (A.C.M.A.); Copperfield Childcare, Claremont, South Africa (H.W.); and the Genetic Immunotherapy Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD (S.S.D.R., H.L.M.).

Background: Allogeneic hematopoietic stem-cell transplantation for X-linked severe combined immunodeficiency (SCID-X1) often fails to reconstitute immunity associated with T cells, B cells, and natural killer (NK) cells when matched sibling donors are unavailable unless high-dose chemotherapy is given. In previous studies, autologous gene therapy with γ-retroviral vectors failed to reconstitute B-cell and NK-cell immunity and was complicated by vector-related leukemia.

Methods: We performed a dual-center, phase 1-2 safety and efficacy study of a lentiviral vector to transfer complementary DNA to bone marrow stem cells after low-exposure, targeted busulfan conditioning in eight infants with newly diagnosed SCID-X1. Read More

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http://www.nejm.org/doi/10.1056/NEJMoa1815408
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http://dx.doi.org/10.1056/NEJMoa1815408DOI Listing
April 2019
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Generation of ERK-independent Human and Non-Human Primate Pluripotent Stem Cells.

Curr Protoc Stem Cell Biol 2019 Apr 17:e85. Epub 2019 Apr 17.

Yale University School of Medicine, New Haven, Connecticut.

The production of human organs inside human-animal interspecies chimeras might one day comprise a viable strategy for generating patient-specific organs, but such experiments will require human chimera-competent pluripotent stem (PS) cells. The stabilization of PS cell self-renewal in serum-free medium and ERK blockade might be critical for capturing primate chimera-competent pluripotency. It has recently been shown that shielding primate cells from the activation of ERK, WNT, and PKC signaling is crucial for deriving African green monkey ERK-independent PS cells. Read More

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http://dx.doi.org/10.1002/cpsc.85DOI Listing

Potential Technologies Review - A hybrid information retrieval framework to accelerate demand-pull innovation in biomedical engineering.

Res Synth Methods 2019 Apr 17. Epub 2019 Apr 17.

Science Management, Institute of Applied Medical Engineering, RWTH Aachen University, Aachen, Germany.

Launching biomedical innovations based on clinical demands instead of translating basic research findings to practice reduces the risk that the results will not fit the clinical routine. To realize this type of innovation, a meta-analysis of the body of research is necessary to reveal demand-matching concepts. However, both the data deluge and the narrow time constraints for innovation make it impossible to perform such reviews manually. Read More

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http://dx.doi.org/10.1002/jrsm.1350DOI Listing

Calcium-induced differentiation in normal human colonoid cultures: Cell-cell / cell-matrix adhesion, barrier formation and tissue integrity.

PLoS One 2019 17;14(4):e0215122. Epub 2019 Apr 17.

Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, United States of America.

Background And Aims: The goal of the study was to assess calcium alone and Aquamin, a multi-mineral natural product that contains magnesium and detectable levels of 72 trace elements in addition to calcium, for capacity to affect growth and differentiation in colonoid cultures derived from histologically-normal human colon tissue.

Methods: Colonoid cultures were maintained in a low-calcium (0.25 mM) medium or in medium supplemented with an amount of calcium (1. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215122PLOS
April 2019
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Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling.

Mol Biol Cell 2019 Apr 17:mbcE18080484. Epub 2019 Apr 17.

Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore-560064, India.

Blood vessel formation requires endothelial cell (EC) migration that depends on dynamic remodeling of the cytoskeleton. Rudhira/Breast Carcinoma Amplified Sequence 3 (BCAS3) is a cytoskeletal protein essential for EC migration and sprouting angiogenesis during mouse development and implicated in metastatic disease. Here, we report that Rudhira mediates cytoskeleton organization and dynamics during EC migration. Read More

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http://dx.doi.org/10.1091/mbc.E18-08-0484DOI Listing

Phylogeny, Distribution, and Pathogenicity of Species Associated With Cankers and Dieback Symptoms of Persian Lime in Mexico.

Plant Dis 2019 Apr 15:PDIS06181036RE. Epub 2019 Apr 15.

5 Laboratorio de Fitopatología, Coordinación Culiacán, Centro de Investigación en Alimentación y Desarrollo, Culiacán, 80110 Sinaloa, Mexico.

Persian lime ( Tan.) is an important and widely cultivated fruit crop in several regions of Mexico. In recent years, severe symptoms of gummosis, stem cankers, and dieback were detected in the Persian lime-producing region in the states of Veracruz and Puebla, Mexico. Read More

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http://dx.doi.org/10.1094/PDIS-06-18-1036-REDOI Listing

The Human Lung Cell Atlas - A high-resolution reference map of the human lung in health and disease.

Am J Respir Cell Mol Biol 2019 Apr 17. Epub 2019 Apr 17.

University of Groningen, University Medical Center Groningen, Pathology and Medical Biology, Groningen, Netherlands.

Lung disease accounts for every sixth death globally. Profiling the molecular state of all lung cell types in health and disease is currently revolutionizing the identification of disease mechanisms and will aid the design of novel diagnostic and personalized therapeutic regimens. Recent progress in high-throughput techniques for single-cell genomic and transcriptomic analyses has opened up new possibilities to study individual cells within a tissue, classify these into cell types, and characterize variations in their molecular profiles as a function of genetics, environment, cell-cell interactions, developmental processes, ageing or disease. Read More

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https://www.atsjournals.org/doi/10.1165/rcmb.2018-0416TR
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http://dx.doi.org/10.1165/rcmb.2018-0416TRDOI Listing
April 2019
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Escherichia coli Strain Designed for Characterizing in Vivo Functions of Nicotinamide Adenine Dinucleotide Analogues.

Org Lett 2019 Apr 17. Epub 2019 Apr 17.

Division of Biotechnology , Dalian Institute of Chemical Physics , CAS, Dalian 116023 , China.

An Escherichia coli strain was constructed for the efficient import of nicotinamide adenine dinucleotide (NAD) analogues into cells by limiting extracellular degradation while expressing an efficient NAD importer. In vivo functions of three NAD analogues were characterized. Nicotinamide hypoxanthine dinucleotide was identified as an inhibitor of NAD synthesis. Read More

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http://dx.doi.org/10.1021/acs.orglett.9b00935DOI Listing

Preparation and performances of the heterostructured material with a Ni-rich layered oxide core and a LiNi0.5Mn1.5O4-like spinel shell.

ACS Appl Mater Interfaces 2019 Apr 17. Epub 2019 Apr 17.

The LiNi1-x-yCoxAlyO2 (NCA) layered materials are regard as a research focus of power LIBs due to their high capacity. However, NCA materials are still up against the defects of cation mixing and surface erosion of electrolyte. Herein, a novel design strategy is proposed to obtain a heterostructured cathode material with high capacity LiNi0. Read More

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http://dx.doi.org/10.1021/acsami.9b01957DOI Listing

The effects of parathyroid hormone-related peptide on cardiac angiogenesis, apoptosis, and function in mice with myocardial infarction.

J Cell Biochem 2019 Apr 17. Epub 2019 Apr 17.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

It is known that parathyroid hormone-related peptide (PTHrP) contains a nuclear localization sequence (NLS, 87-107), which, together with its C-terminus (107-139), has been shown to positively regulate vascular smooth muscle cell (VSMCs) proliferation and vascular neointima formation, and inhibit cellular apoptosis. The role of PTHrP in ischemic cardiac diseases remains unclear. In this study, we attempted to determine whether PTHrP 87 to 139 can play a role in promoting cardiac function via enhancing angiogenesis after myocardial infarction (MI) occurred. Read More

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http://dx.doi.org/10.1002/jcb.28735DOI Listing

Endometriosis.

Endocr Rev 2019 Apr 17. Epub 2019 Apr 17.

Departments of Obstetrics and Gynecology, Northwestern University, Chicago, IL.

Pelvic endometriosis is a complex syndrome characterized by an estrogen-dependent chronic inflammatory process that affects primarily pelvic tissues, including the ovaries. It is caused when shed endometrial tissue travels retrograde into the lower abdominal cavity. Endometriosis is the most common cause of chronic pelvic pain in women and is associated with infertility. Read More

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http://dx.doi.org/10.1210/er.2018-00242DOI Listing

Interrater Reliability of Clinical Grading Measures for Cutaneous Chronic Graft-vs-Host Disease.

JAMA Dermatol 2019 Apr 17. Epub 2019 Apr 17.

Department of Dermatology, Duke University, Durham, North Carolina.

Importance: Cutaneous chronic graft-vs-host disease (cGVHD) is common after allogeneic hematopoietic stem cell transplant and is often associated with poor patient outcomes. A reliable and practical method for assessing disease severity and response to therapy among these patients is urgently needed.

Objective: To evaluate the interrater agreement and reliability of skin-specific and range of motion (ROM) variables of the 2014 National Institutes of Health (NIH) response criteria for cGVHD and a skin sclerosis grading scale (SSG). Read More

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http://dx.doi.org/10.1001/jamadermatol.2018.5459DOI Listing

The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats.

J Appl Oral Sci 2019 11;27:e20180103. Epub 2019 Apr 11.

Universidade Federal do Piauí, Departamento de Patologia e Prática Odontológica, Teresina, Piauí, Brasil.

Objective: This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera.

Material And Methods: Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. Read More

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http://dx.doi.org/10.1590/1678-7757-2018-0103DOI Listing

[Effect of polyethylene terephthalate (Dacron®) on functional potential of human bone-marrow/progenitor stem cells].

Angiol Sosud Khir 2019 ;25(1):25-30

National Medical Research Centre named after Academician E.N. Meshalkin under the RF Ministry of Public Health, Novosibirsk, Russia.

Vascular grafts made of polytetrafluoroethylene and polyethylene terephthalate have widely been used in cardiovascular surgery. The causes of delayed colonization of such grafts by endotheliocytes and mesenchymal stem cells have not been adequately investigated. The authors examined the effect of polyethylene terephthalate on the functional activity of human bone marrow mesenchymal stem cells and endothelial progenitor cells in vitro. Read More

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http://dx.doi.org/10.33529/angio2019103DOI Listing
January 2019

A Case of Childhood Blastic Phase Chronic Myeloid Leukemia With Minor BCR-ABL.

J Pediatr Hematol Oncol 2019 Apr 15. Epub 2019 Apr 15.

Department of Pediatrics, National Center for Global Health and Medicine.

Chronic myeloid leukemia (CML) is commonly associated with major BCR-ABL transcript. We present a child with blastic phase CML associated with minor BCR-ABL transcript without prior CML diagnosis. Diagnosis was achieved by fluorescence in situ hybridization of peripheral blood neutrophils, which identified 90% as BCR-ABL positive. Read More

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http://dx.doi.org/10.1097/MPH.0000000000001488DOI Listing
April 2019
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Concentration of Chondrogenic Soluble Factors in Freshly Harvested Lipoaspirate.

Ann Plast Surg 2019 Apr 15. Epub 2019 Apr 15.

From the Department of Plastic Surgery, Hand Surgery-Burn Center University Hospital RWTH Aachen, Aachen, Germany.

Background: Cartilage tissue has a limited capacity for healing with the consequence that patients are often treated symptomatically until they become candidates for osteotomy or total joint replacement. Alternative biological therapies, for example, application of platelet-rich plasma and implantation of chondrocytes and mesenchymal stem cells, have emerged as a new treatment modality to repair articular cartilage. In addition, autologous fat transfer is performed for treatment of cartilage defects, example given, in osteoarthrosis, but several questions regarding basic biochemical properties of the transplant remain unanswered. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001936DOI Listing

Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.

Regen Med 2019 Mar 27;14(3):165-177. Epub 2019 Feb 27.

Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

To determine the efficiency of mesenchymal stem cells (MSCs) of different sources on airway epithelial cells regeneration and track where and to what extent transplanted MSCs home to injured tissues. We performed DiO-labeled human bone marrow-derived MSCs (hBMSCs) or human chorionic villi-derived MSCs transplantation studies using naphthalene-induced airway injury animal models. Compared with human chorionic villi-derived MSCs, hBMSCs facilitated airway epithelium regeneration faster and better from day 5 after transplantation; moreover, more transplanted hBMSCs distributed in injured lung tissues at the early stage of postinjury, which was mediated by C-X-C motif chemokine ligand 12. Read More

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http://dx.doi.org/10.2217/rme-2018-0152DOI Listing

[Effects of Chinese milk vetch intercropped with rape under straw mulching on soil aggregate and organic carbon character].

Ying Yong Sheng Tai Xue Bao 2019 Apr;30(4):1235-1242

College of Agronomy and Biotechnology, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education/Engineering Research Center of South Upland Agriculture, Ministry of Education, Beibei 400716, Chongqing, China.

There are severe soil erosion, shallow soil, reduction of soil organic matter, and poor soil and water conservation in purple soil areas in Southwest China, which become the main limiting factors for the sustainable development of agriculture. A series of buckets and field experiments in the field were employed to explore the soil aggregate and soil organic carbon in response to Chinese milk vetch intercropped with rape under straw mulching, aiming to improve soil aggregate structure and increase organic carbon content. Results showed that intercropped Chinese milk vetch increased soil micro-aggregate content in rape rhizosphere, and reduced soil aggregate mean mass diameter. Read More

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http://dx.doi.org/10.13287/j.1001-9332.201904.006DOI Listing

[Regulation effects of irrigation methods and nitrogen application on soil water, nitrate, and wheat growth and development].

Ying Yong Sheng Tai Xue Bao 2019 Apr;30(4):1161-1169

Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen 041000, Shanxi, China.

Field experiments were conducted to examine the effects of flooding irrigation (FI), micro-sprinkler irrigation (SI), drip irrigation (DI), combined with nitrogen application (N:157.5 kg·hm as basal, 67.5 kg·hm top dressed at jointing stage; N:157. Read More

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http://dx.doi.org/10.13287/j.1001-9332.201904.034DOI Listing

[Effects of combined application of pig manure with urea on grain yield and nitrogen utilization efficiency in rice-wheat rotation system].

Ying Yong Sheng Tai Xue Bao 2019 Apr;30(4):1088-1096

College of Resource Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

To examine the effects of pig manure application on dry matter production, nitrogen accumulation and distribution, grain yield and nitrogen use efficiency of rice and wheat, the field trial was conducted with wheat 863 and rice 498. Fertilization treatments consisted of seven rates of organic manure supply: control (CK, no chemical nitrogen fertilizer, no pig manure), conventional fertilizing (T, no pig manure), 2500 kg·hm pig manure with 75% conventional fertilizing (T), 5000 kg·hm pig manure with 50% conventional fertilizing (T), 10000 kg·hm pig manure (T), 15000 kg·hm pig manure (T) and 20000 kg·hm pig manure (T). Combined application of pig manure with chemical fertilizer promoted dry matter accumulation of rice and wheat throughout the growing season. Read More

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http://dx.doi.org/10.13287/j.1001-9332.201904.031DOI Listing

Comprehensive analysis of isolated der(1;7)(q10;p10) in a large international homogenous cohort of patients with myelodysplastic syndromes.

Genes Chromosomes Cancer 2019 Apr 17. Epub 2019 Apr 17.

Clinics of Hematology and Medical Oncology, University Medical Center Göttingen, Göttingen, Germany.

The karyotype is a strong independent prognostic factor in myelodysplastic syndromes (MDS). Since the implementation of the new comprehensive cytogenetic scoring system for MDS, chromosome 7 anomalies are no longer generally assigned to poor risk features but are thoroughly separated. However, der(1;7)(q10;p10), hereinafter der(1;7), is merged into the group labelled "any other single" and belongs to the intermediate risk group, just by definition due to lack of adequate clinical data. Read More

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http://dx.doi.org/10.1002/gcc.22760DOI Listing

Early clear cell "sugar" lung cancer management: A case report and a brief literature review.

Thorac Cancer 2019 Apr 16. Epub 2019 Apr 16.

UOC of Oncology, University of Rome, Aprilia, Italy.

A clear cell tumor is a histological entity that rarely originates outside of the kidney. We describe a rare case of a clear cell tumor of the lung, also known as "sugar cancer," that occurred in a 74 year-old male patient, and perform a brief literature review. This report highlights the importance of an adequate disease management team, including surgeons, oncologists, and pathologists, to identify the best therapeutic approach to improve survival rates and the quality of life of patients affected by this rare disease. Read More

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http://dx.doi.org/10.1111/1759-7714.13077DOI Listing

Integrating genetic mutations and expression profiles for survival prediction of lung adenocarcinoma.

Thorac Cancer 2019 Apr 16. Epub 2019 Apr 16.

Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Lung adenocarcinoma (LUAD) is a set of heterogeneous diseases with distinct genetic and transcriptomic characteristics. Since the introduction of the 2011 International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society histologic classification, increasing evidence has provided insights into genomic mutations and rearrangements among individual histologic subtypes of LUAD. However, how genotypic and phenotypic features of LUAD are interconnected is not well understood. Read More

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http://dx.doi.org/10.1111/1759-7714.13072DOI Listing

Epidemiology of viral infections among children undergoing hematopoietic stem cell transplant: Α prospective single- center study.

Transpl Infect Dis 2019 Apr 16:e13095. Epub 2019 Apr 16.

Pediatric Clinic, "P & A Kyriakou" Children's Hospital, National and Kapodistrian University of Athens, Faculty of Nursing, Athens, Greece.

Background: Viral infections are a significant cause of morbidity and mortality in pediatric transplant populations. We analyzed the epidemiology of viral infections in pediatric hematopoietic stem cell transplant (HSCT) patients, including their incidence, associated risk factors, and outcome.

Methods: In a prospective study from September 2011 to September 2015 blood, urine and stool specimens were monitored weekly from transplantation to day 100 or after if clinically suspected, by use of real-time polymerase chain reaction. Read More

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http://dx.doi.org/10.1111/tid.13095DOI Listing

NK cells: An attractive candidate for cancer therapy.

J Cell Physiol 2019 Apr 16. Epub 2019 Apr 16.

Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.

Natural killer (NK) cells have significant capability in tumor immune-surveillance. The ability of lyse transformed cells immediately in an antigen-independent manner make them an attractive candidate for cancer cell therapy. Despite employment of NK cells in cancer immunotherapy, clinical trials are faced with serious limitations such as trouble with the penetration of NK cells in tumor sites, limited in vivo persistence, and tumor microenvironment interference. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28657
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http://dx.doi.org/10.1002/jcp.28657DOI Listing
April 2019
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Reconstruction of lymphatic vessels in the mouse tail after cupping therapy.

Folia Morphol (Warsz) 2019 Apr 17. Epub 2019 Apr 17.

Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine, Yantai, China.

Background: To investigate the regulatory mechanism of local lymphatic reconstruction after cupping therapy in a mouse model.

Materials And Methods: The lymphatic reconstruction process in the mouse tail after cupping therapy as well as the expression levels of the vascular endothelial identification molecule CD34, prospero homeobox protein 1 (PROX1), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) were investigated for a duration of 4 days through immunohistochemistry experiments.

Results: On day 1 after cupping therapy, the CD34+ and LYVE-1+ cell densities were significantly increased, and the formed CD34+LYVE-1+ tubular structure started to express PROX1. Read More

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https://journals.viamedica.pl/folia_morphologica/article/vie
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http://dx.doi.org/10.5603/FM.a2019.0044DOI Listing
April 2019
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Cancer Stem Cells: Acquisition, Characteristics, Therapeutic Implications, Targeting Strategies and Future Prospects.

Stem Cell Rev 2019 Apr 16. Epub 2019 Apr 16.

Amity Institute of Biotechnology, Amity University, Mumbai-Pune Expressway, Bhatan, Panvel, Mumbai, Maharashtra, 410206, India.

Since last two decades, the major cancer research has focused on understanding the characteristic properties and mechanism of formation of Cancer stem cells (CSCs), due to their ability to initiate tumor growth, self-renewal property and multi-drug resistance. The discovery of the mechanism of acquisition of stem-like properties by carcinoma cells via epithelial-mesenchymal transition (EMT) has paved a way towards a deeper understanding of CSCs and presented a possible avenue for the development of therapeutic strategies. In spite of years of research, various challenges, such as identification of CSC subpopulation, lack of appropriate experimental models, targeting cancer cells and CSCs specifically without harming normal cells, are being faced while dealing with CSCs. Read More

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http://link.springer.com/10.1007/s12015-019-09887-2
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http://dx.doi.org/10.1007/s12015-019-09887-2DOI Listing
April 2019
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Midkine silencing enhances the anti-prostate cancer stem cell activity of the flavone apigenin: cooperation on signaling pathways regulated by ERK, p38, PTEN, PARP, and NF-κB.

Invest New Drugs 2019 Apr 16. Epub 2019 Apr 16.

Department of Histology and Embryology, School of Medicine, Istanbul Aydin University, Istanbul, Turkey.

Prostate cancer (PCa) is the most common cancer in men worldwide. Midkine (MK) is overexpressed in PCa, as well as in tumor-initiating cells termed cancer stem cells (CSCs). Apigenin is a dietary flavone with considerable anti-tumor activities. Read More

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http://dx.doi.org/10.1007/s10637-019-00774-8DOI Listing

The N-end rule pathway enzyme Naa10 supports epiblast specification in mouse embryonic stem cells by modulating FGF/MAPK.

In Vitro Cell Dev Biol Anim 2019 Apr 16. Epub 2019 Apr 16.

Department of Integrated Basic Medical Science, Division of Functional Histology, Ehime University School of Medicine, Shitsukawa, Toon, Ehime, 791-0295, Japan.

N-terminal acetylation (Nt-acetylation) refers to the acetylation of the free α-amino group at the N-terminus of a polypeptide. While the effects of Nt-acetylation are multifaceted, its most known function is in the acetylation-dependent N-end rule protein degradation pathway (Ac/N-end rule pathway), where Nt-acetylation is recognized as a degron by designated E3 ligases, eventually leading to target degradation by the ubiquitin-proteasome system. Naa10 is the catalytic subunit of the major Nt-acetylation enzyme NatA, which Nt-acetylates proteins whose second amino acid has a small side chain. Read More

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http://dx.doi.org/10.1007/s11626-019-00341-8DOI Listing

Efficacy of mechanical vibration in regulating mesenchymal stem cells gene expression.

In Vitro Cell Dev Biol Anim 2019 Apr 15. Epub 2019 Apr 15.

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

This study aimed at investigating the expression of osteoblast and chondrocyte-related genes in mesenchymal stem cells (MSCs), derived from rabbit adipose tissue, under mechanical vibration. The cells were placed securely on a vibrator's platform and subjected to 300 Hz of sinusoidal vibration, with a maximum amplitude of 10 μm, for 45 min per day, and for 14 consequent days, in the absence of biochemical reagents. The negative control group was placed in the conventional culture medium with no mechanical loading. Read More

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http://dx.doi.org/10.1007/s11626-019-00340-9DOI Listing

In Vitro Study of the Enzymatic and Nonenzymatic Conjugation of Treosulfan with Glutathione.

Eur J Drug Metab Pharmacokinet 2019 Apr 16. Epub 2019 Apr 16.

Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 6 Święcickiego Street, 60-781, Poznań, Poland.

Background And Objectives: Treosulfan (dihydroxybusulfan), licensed for the treatment of ovarian carcinoma, is investigated in clinical trials as a myeloablative agent for conditioning prior to hematopoietic stem cell transplantation. Clinical experience shows that treosulfan exhibits lower organ toxicity than busulfan, including hepatotoxicity. Elimination of busulfan primarily via enzymatic conjugation with glutathione (GSH) in the liver is considered to be the main cause of the drug's hepatotoxicity and interpatient clearance variability. Read More

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http://dx.doi.org/10.1007/s13318-019-00555-xDOI Listing

Quantitative Phenotyping of Cell-Cell Junctions to Evaluate ZO-1 Presentation in Brain Endothelial Cells.

Ann Biomed Eng 2019 Apr 15. Epub 2019 Apr 15.

Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.

The selective permeability of the blood-brain barrier (BBB) is controlled by tight junction-expressing brain endothelial cells. The integrity of these junctional proteins, which anchor to actin via zonula occludens (e.g. Read More

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http://dx.doi.org/10.1007/s10439-019-02266-5DOI Listing

Lectins and ELLSA as powerful tools for glycoconjugate recognition analyses.

Glycoconj J 2019 Apr 16. Epub 2019 Apr 16.

Ta-Cheng Stem Cell Therapy Center, National Taiwan University, Taipei, Taiwan.

Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. Read More

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http://dx.doi.org/10.1007/s10719-019-09865-3DOI Listing

Epigenetic Modulators as Potential Multi-targeted Drugs Against Hedgehog Pathway for Treatment of Cancer.

Protein J 2019 Apr 16. Epub 2019 Apr 16.

Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Gram-Lavale, Taluka-Mulshi, Pune, 412115, India.

The Sonic hedgehog signalling is known to play a crucial role in regulating embryonic development, cancer stem cell maintenance and tissue patterning. Dysregulated hedgehog signalling has been reported to affect tumorigenesis and drug response in various human malignancies. Epigenetic therapy relying on DNA methyltransferase and Histone deacetylase inhibitors are being proposed as potential drug candidates considering their efficiency in preventing development of cancer progenitor cells, killing drug resistant cells and also dictating "on/off" switch of tumor suppressor genes and oncogenes. Read More

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http://link.springer.com/10.1007/s10930-019-09832-9
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http://dx.doi.org/10.1007/s10930-019-09832-9DOI Listing
April 2019
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The prognostic impact of the cytomegalovirus serostatus in patients with chronic hematological malignancies after allogeneic hematopoietic stem cell transplantation: a report from the Infectious Diseases Working Party of EBMT.

Ann Hematol 2019 Apr 16. Epub 2019 Apr 16.

Collegium Medicum UMK, University Hospital, Bydgoszcz, Poland.

It has been shown recently that donor and/or recipient cytomegalovirus (CMV) seropositivity is associated with a significant overall survival (OS) decline in acute leukemia patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT). We now analyzed the prognostic impact of the donor/recipient CMV serostatus in 6968 patients with chronic hematological malignancies who underwent allo-HSCT. Donor and/or recipient CMV seropositivity was associated with a significantly reduced 2-year progression-free survival (PFS, 50% vs. Read More

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http://link.springer.com/10.1007/s00277-019-03669-z
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http://dx.doi.org/10.1007/s00277-019-03669-zDOI Listing
April 2019
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The benefit of chronic graft-versus-host disease in patients with acute myeloid leukemia relapsed after allogeneic stem cell transplantation.

Ann Hematol 2019 Apr 16. Epub 2019 Apr 16.

Department of Hematology, Changhai Hospital, The Second Military Medical University, Shanghai, China.

To investigate the effect of chronic graft-versus-host disease (cGVHD) on the outcomes of acute myeloid leukemia (AML) patients who relapsed after allogenic hematopoietic cell transplantation, we performed a retrospective analysis on 218 patients with a median follow-up of 21.4 (3.4-179. Read More

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http://dx.doi.org/10.1007/s00277-019-03682-2DOI Listing

Allogeneic donor split skin grafts for treatment of refractory ulcers in cutaneous chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation-a retrospective analysis on seven patients.

Ann Hematol 2019 Apr 16. Epub 2019 Apr 16.

Department of Plastic, Reconstructive and Hand Surgery, University Hospital, Regensburg, Germany.

Refractory skin ulcers due to severe chronic graft-versus-host disease (cGVHD) remain to be associated with significant morbidity and mortality.We performed an allogeneic donor skin transplantation in seven adult patients after allogeneic hematopoietic stem cell transplantation for cGVHD-associated refractory skin ulcers. While four patients received a split skin graft (SSG), in one patient, a full thickness skin graft for two small refractory ulcers of the ankle was performed, and one patient received in vitro expanded donor keratinocyte grafts derived from hair roots of the original unrelated donor. Read More

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http://dx.doi.org/10.1007/s00277-019-03687-xDOI Listing

Integrating transcriptome-wide association study and mRNA expression profiling identifies novel genes associated with bone mineral density.

Osteoporos Int 2019 Apr 15. Epub 2019 Apr 15.

Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, No.76 Yan Ta West Road, Xi'an, 710061, People's Republic of China.

To scan novel candidate genes associated with osteoporosis, a two-stage transcriptome-wide association study (TWAS) of bone mineral density (BMD) was conducted. The BMD-associated genes identified by TWAS were then compared with the gene expression profiling of BMD in bone cells, B cells, and mesenchymal stem cells. We identified multiple candidate genes and gene ontology (GO) terms associated with BMD. Read More

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http://dx.doi.org/10.1007/s00198-019-04958-zDOI Listing

The unethicality of doping in sports.

Clin Ter 2019 Mar-Apr;170(2):e100-e101

School of Law, University of Camerino, Camerino, Italy.

Performance enhancing drug use in sport arguably constitutes a crisis, and as such, targeted measures are needed in order to stem it. Substance abuse in professional sports and competitions has besmirched many world-class athletes' reputations and standing, in addition to jeopardizing their health. Furthermore, there are many instances of amateur athletes and school-aged competitors who have taken to using such substances as well, significantly exacerbating the overall picture. Read More

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http://dx.doi.org/10.7417/CT.2019.2117DOI Listing
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