507,052 results match your criteria Stem cells Dayton Ohio[Journal]


Individual radiation protection: idea and research needs.

J Radiol Prot 2019 Feb 22. Epub 2019 Feb 22.

Deputy Director General Office, Federal Authority for Nuclear Regulation (FANR), The Landmark Tower Al Markaziyah West - Abu Dhabi Corniche - First Floor Abu Dhabi, United Arab Emirates, Abu Dhabi, Abu Dhabi, 112021, UNITED ARAB EMIRATES.

The current Radiation Protection System is based on the definition and use of the "effective dose", a complex quantity which is not measurable and normally not easily understood by the general public. National radiation protection requirements and regulations stem from a few basic principles, one of which is the respect of individual annual dose limits, specified in terms of the quantities "effective dose" and "equivalent dose". Another guiding principle is the continuous quest for optimization of individual integrated doses, i. Read More

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http://dx.doi.org/10.1088/1361-6498/ab0979DOI Listing
February 2019

Molecular beam epitaxial growth of dilute nitride GaNAs and GaInNAs nanowires.

Nanotechnology 2019 Feb 22. Epub 2019 Feb 22.

Ehime University Faculty of Engineering Graduate School of Science and Engineering, Matsuyama, Ehime, JAPAN.

We report the growth of dilute nitride GaNAs and GaInNAs core-multishell nanowires by plasma-assisted molecular beam epitaxy. Using constituent Ga-induced vapor-liquid-solid growth, these nanowires were grown on Si(111) and silicon on insulator (SOI) substrates. The GaNAs shell nominally contains 0%, 2%, and 3% nitrogen. Read More

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http://dx.doi.org/10.1088/1361-6528/ab0974DOI Listing
February 2019

The optimum temperature for the heat therapy for meibomian gland dysfunction.

Authors:
Douglas Borchman

Ocul Surf 2019 Feb 19. Epub 2019 Feb 19.

Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY, 40202, USA. Electronic address:

Purpose: Numerous devices have been developed to warm the eyelid as a therapy for Meibomian gland dysfunction. The optimum temperature for such therapy was determined.

Methods: Meibum lipid disorder versus temperature was calculated from previously published phase transition parameters measured using infrared spectroscopy. Read More

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http://dx.doi.org/10.1016/j.jtos.2019.02.005DOI Listing
February 2019

Autologous Transplantation for Male Germ Cell Tumors: Improved Outcomes over 3 decades.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Medical College of Wisconsin, Milwaukee, WI.

Purpose: The curative potential of autologous hematopoietic cell transplantation (autoHCT) for male germ cell tumors (GCT) is well established. Optimal timing and number (single, ST vs. tandem, TT vs. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.015DOI Listing
February 2019

AUTOLOGOUS HEMATOPOIETIC CELL TRANSPLANTATION FOR TREATMENT-REFRACTORY RELAPSING MULTIPLE SCLEROSIS: POSITION STATEMENT FROM THE AMERICAN SOCIETY FOR BLOOD AND MARROW TRANSPLANTATION.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Multiple sclerosis (MS) is a chronic, disabling, immune-mediated, central nervous system demyelinating and degenerative disease. Approved disease modifying therapies may be incompletely effective in some patients with highly active relapsing disease and high risk of disability. Immunoablative or myeloablative therapy followed by autologous hematopoietic cell transplantation (AHCT) has been investigated in retrospective studies, clinical trials, and meta-analyses/systematic reviews as an approach to address this unmet clinical need. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.014DOI Listing
February 2019

Risk factors and predictive scoring system for post-transplant lymphoproliferative disorder after hematopoietic stem cell transplantation.

Biol Blood Marrow Transplant 2019 Feb 19. Epub 2019 Feb 19.

Department of Oncology and Hematology, Shimane University Hospital, Izumo, Japan. Electronic address:

We analyzed data of 64,539 consecutive patients in the national Japanese transplant registry, including 40,195 after allogeneic stem cell transplantation, 24,215 after autologous stem cell transplantation, and 129 after syngeneic stem cell transplantation, and 299 developed Epstein-Barr virus positive post-transplant lymphoproliferative disorder. The probability at 2 years was 0.79% after allogeneic transplantation, 0. Read More

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http://dx.doi.org/10.1016/j.bbmt.2019.02.016DOI Listing
February 2019

Malus pumila and Juglen regia plant species mediated zinc oxide nanoparticles: Synthesis, spectral characterization, antioxidant and antibacterial studies.

Microb Pathog 2019 Feb 19. Epub 2019 Feb 19.

Material Research Laboratory, Department of Chemistry, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address:

Zinc oxide nanoparticles derived from Malus pumila (apple) and Juglen regia (walnut) plant is an attractive area of research because of their widespread use. The use of plant material to synthesize zinc oxide nanoparticles has been considered as one of the best environmentally friendly approach. This method appears to be low-cost as compare to other conventional method of synthesis. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.02.020DOI Listing
February 2019

Divergent Response to Cannabinoid Receptor Stimulation in High and Low Stress-Induced Analgesia Mouse Lines Is Associated with Differential G-Protein Activation.

Neuroscience 2019 Feb 19. Epub 2019 Feb 19.

Faculty of Pharmacy with the Laboratory Medicine Division, Department of Pharmacodynamics, Medical University of Warsaw, Centre for Preclinical Research and Technology, Warsaw, Poland; Laboratory of Neurogenomics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland; Department of Internal Medicine, Hypertension and Vascular Diseases, Medical University of Warsaw, Warsaw, Poland.

Bidirectional selection of mice for high (HA) and low (LA) swim stress-induced analgesia (SSIA) is associated with a divergent response to opioids. In the current study, we investigated whether the genetic divergence in opioid system activity between HA and LA mice also affects cannabinoid sensitivity. Additionally, we also investigated whether the endocannabinoid system mediates SSIA in these lines. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.02.015DOI Listing
February 2019

Mechanical influences on Cardiovascular Differentiation and Disease Modeling.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037. Electronic address:

Tissues are continuously exposed to forces in vivo, whether from fluid pressure in an artery from our blood or compressive forces on joints from our body weight. The forces that cells are exposed to arise almost immediately after conception; it is therefore important to understand how forces shape stem cell differentiation into lineage committed cells, how they help organize cells into tissues, and how forces can cause or exacerbate disease. No tissue is exempt, but cardiovascular tissues in particular are exposed to these forces. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.019DOI Listing
February 2019

Interleukin-6 released from differentiating human beige adipocytes improves browning.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, University of Debrecen, Faculty of Medicine, Debrecen, Hungary; MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary. Electronic address:

Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL) -6, IL-8 and monocyte chemoattractant protein (MCP) -1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.015DOI Listing
February 2019

FXR Regulates Intestinal Cancer Stem Cell Proliferation.

Cell 2019 Feb;176(5):1098-1112.e18

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address:

Increased levels of intestinal bile acids (BAs) are a risk factor for colorectal cancer (CRC). Here, we show that the convergence of dietary factors (high-fat diet) and dysregulated WNT signaling (APC mutation) alters BA profiles to drive malignant transformations in Lgr5-expressing (Lgr5) cancer stem cells and promote an adenoma-to-adenocarcinoma progression. Mechanistically, we show that BAs that antagonize intestinal farnesoid X receptor (FXR) function, including tauro-β-muricholic acid (T-βMCA) and deoxycholic acid (DCA), induce proliferation and DNA damage in Lgr5 cells. Read More

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http://dx.doi.org/10.1016/j.cell.2019.01.036DOI Listing
February 2019

Fabrication and Evaluation of Bio-based Nanocomposite TFC Hollow Fiber Membranes for Enhanced CO2 capture.

ACS Appl Mater Interfaces 2019 Feb 22. Epub 2019 Feb 22.

Nanocellulose is a promising and sustainable bio-based nanomaterial due to its excellent mechanical properties, biocompatibility, natural abundance, and especially its high aspect ratio. Interest in applying nanocellulose as nanofillers in membrane fabrication has been growing rapidly in recent years. In the present work, nanocellulose crystals (CNC) and nanocellulose fibers (CNF) were incorporated into polyvinyl alcohol (PVA) to prepare evenly dispersed nanocomposite. Read More

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http://dx.doi.org/10.1021/acsami.8b19651DOI Listing
February 2019

Wnt traffic from endoplasmic reticulum to filopodia.

PLoS One 2019 22;14(2):e0212711. Epub 2019 Feb 22.

Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore.

Wnts are a family of secreted palmitoleated glycoproteins that play key roles in cell to cell communication during development and regulate stem cell compartments in adults. Wnt receptors, downstream signaling cascades and target pathways have been extensively studied while less is known about how Wnts are secreted and move from producing cells to receiving cells. We used the synchronization system called Retention Using Selective Hook (RUSH) to study Wnt trafficking from endoplasmic reticulum to Golgi and then to plasma membrane and filopodia in real time. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212711PLOS
February 2019

HEWL interacts with dissipated oleic acid micelles, and decreases oleic acid cytotoxicity.

PLoS One 2019 22;14(2):e0212648. Epub 2019 Feb 22.

State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Institute of Photonics and Photon-Technology, Northwest University, Xi'an, China.

Senile plaques are well-known hallmarks of Alzheimer's Diseases (AD). However, drugs targeting tangles of the protein tau and plaques of β-amyloid have no significant effect on disease progression, and the studies on the underlying mechanism of AD remain in high demand. Growing evidence supports the protective role of senile plaques in local inflammation driven by S100A9. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212648PLOS
February 2019

Earlier relapse detection after allogeneic haematopoietic stem cell transplantation by chimerism assays: Digital PCR versus quantitative real-time PCR of insertion/deletion polymorphisms.

PLoS One 2019 22;14(2):e0212708. Epub 2019 Feb 22.

Haematology Department, Hospital Regional Universitario de Málaga, Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain.

Background: The analysis of molecular haematopoietic chimerisms (HC) has become a well-established method to monitor the transplant evolution and to assess the risk of relapse after allogeneic stem cells transplantation (allo-STC). Different techniques and molecular markers are being used for chimerism surveillance after transplantation, including quantitative real-time PCR (qPCR) and the recently developed digital PCR (dPCR). This study aims to compare the sensitivity and accuracy of both methods to quantify HC and predict early relapse. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212708PLOS
February 2019

Autophagic flux modulation by Wnt/β-catenin pathway inhibition in hepatocellular carcinoma.

PLoS One 2019 22;14(2):e0212538. Epub 2019 Feb 22.

Department of Surgery, Transplant Center, College of Medicine, University of Kentucky, Lexington, Kentucky, United States of America.

Autophagy targets cellular components for lysosomal-dependent degradation in which the products of degradation may be recycled for protein synthesis and utilized for energy production. Autophagy also plays a critical role in cell homeostasis and the regulation of many physiological and pathological processes and prompts this investigation of new agents to effect abnormal autophagy in hepatocellular carcinoma (HCC). 2,5-Dichloro-N-(2-methyl-4-nitrophenyl) benzenesulfonamide (FH535) is a synthetic inhibitor of the Wnt/β-catenin pathway that exhibits anti-proliferative and anti-angiogenic effects on different types of cancer cells. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212538PLOS
February 2019

Capturing the diversity of working age life-courses: A European perspective on cohorts born before 1945.

PLoS One 2019 22;14(2):e0212400. Epub 2019 Feb 22.

Faculty of Social Sciences, University of Helsinki, Helsinki, Finland.

Life-courses describe people's activities from the cradle to the grave. Because life-courses are typically complex, models are used to simplify their description. The most commonly used model is tripartite, representing lives in subsequent periods of education, work, and retirement. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212400PLOS
February 2019

Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies.

PLoS Biol 2019 Feb 22;17(2):e3000016. Epub 2019 Feb 22.

Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Studying aneuploidy during organism development has strong limitations because chronic mitotic perturbations used to generate aneuploidy usually result in lethality. We developed a genetic tool to induce aneuploidy in an acute and time-controlled manner during Drosophila development. This is achieved by reversible depletion of cohesin, a key molecule controlling mitotic fidelity. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000016DOI Listing
February 2019

Microglia exit the CNS in spinal root avulsion.

PLoS Biol 2019 Feb 22;17(2):e3000159. Epub 2019 Feb 22.

Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, United States of America.

Microglia are central nervous system (CNS)-resident cells. Their ability to migrate outside of the CNS, however, is not understood. Using time-lapse imaging in an obstetrical brachial plexus injury (OBPI) model, we show that microglia squeeze through the spinal boundary and emigrate to peripheral spinal roots. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000159DOI Listing
February 2019

IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment.

J Histochem Cytochem 2019 Feb 22:22155419833334. Epub 2019 Feb 22.

UD of Biochemistry and Molecular Biology.

Glioblastoma (GB) is the most frequently occurring and aggressive primary brain tumor. Glioma stem cells (GSCs) and astrocytoma cells are the predominant malignant cells occurring in GB besides a highly heterogeneous population of migrating, neovascularizing and infiltrating myeloid cells that forms a complex tumor microenvironment (TME). Cross talk between the TME cells is pivotal in the biology of this tumor and, consequently, adaptor proteins at critical junctions of signaling pathways may be crucial. Read More

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http://dx.doi.org/10.1369/0022155419833334DOI Listing
February 2019

Methylation-based biological age and breast cancer risk.

J Natl Cancer Inst 2019 Feb 22. Epub 2019 Feb 22.

Epidemiology Branch, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC.

Background: Age is one of the strongest predictors of cancer, chronic disease and mortality, but biological responses to aging differ among people. Epigenetic DNA modifications have been used to estimate "biological age," which may be a useful predictor of disease risk. We test this hypothesis for breast cancer. Read More

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http://dx.doi.org/10.1093/jnci/djz020DOI Listing
February 2019

Can We Avoid Implant-selection Errors in Total Joint Arthroplasty?

Clin Orthop Relat Res 2019 Jan;477(1):130-133

M. P. Ast, Hospital for Special Surgery, New York, NY, USA D. J. Mayman, M. P. Bostrom, A. Gonzalez Della Valle, S. B. Haas, Hospital for Special Surgery, New York, NY.

Background: Implant selection in the operating room is a manual process. This manual process combined with complex compatibility rules and inconsistent implant labeling may lead to implant-selection errors. These might be reduced using an automated process; however, little is known about the efficacy of available automated error-reduction systems in the operating room. Read More

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http://dx.doi.org/10.1097/CORR.0000000000000536DOI Listing
January 2019

Understanding Youths' Ability to Interpret 3D-Printed Physical Activity Data and Identify Associated Intensity Levels: Mixed-Methods Study.

J Med Internet Res 2019 Feb 22;21(2):e11253. Epub 2019 Feb 22.

Applied Sports Science Technology and Medicine Research Centre (A-STEM), Swansea University, Swansea, United Kingdom.

Background: A significant proportion of youth in the United Kingdom fail to meet the recommended 60 minutes of moderate-to-vigorous physical activity every day. One of the major barriers encountered in achieving these physical activity recommendations is the perceived difficulty for youths to interpret physical activity intensity levels and apply them to everyday activities. Personalized physical activity feedback is an important method to educate youths about behaviors and associated outcomes. Read More

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http://dx.doi.org/10.2196/11253DOI Listing
February 2019

The proneural wave in the optic lobe is driven by an excitable reaction-diffusion mechanism.

Elife 2019 Feb 22;8. Epub 2019 Feb 22.

Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, United Kingdom.

In living organisms, self-organised waves of signalling activity propagate spatiotemporal information within tissues. During the development of the largest component of the visual processing centre of the brain, a travelling wave of proneural gene expression initiates neurogenesis in the larval optic lobe primordium and drives the sequential transition of neuroepithelial cells into neuroblasts. Here, we propose that this 'proneural wave' is driven by an excitable reaction-diffusion system involving epidermal growth factor receptor (EGFR) signalling interacting with the proneural gene . Read More

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http://dx.doi.org/10.7554/eLife.40919DOI Listing
February 2019

Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells.

Tissue Eng Part C Methods 2019 Feb 22. Epub 2019 Feb 22.

Swansea University, 7759, Swansea, West Glamorgan, United Kingdom of Great Britain and Northern Ireland ;

The umbilical cord offers a source of readily available mesenchymal stromal cells for use in research and ultimately therapeutic application. However, methods of isolating these cells vary between investigators, and no standard method has been adopted. The aims of this work were to i) develop a methodology for the isolation of umbilical cord matrix cells without the use of enzymatic digestion or complicated dissection; ii) investigate the use of pooled maternal serum as a media supplement; and iii) to demonstrate that the cells isolated were mesenchymal stromal cells. Read More

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http://dx.doi.org/10.1089/ten.TEC.2019.0008DOI Listing
February 2019

Partial Transformation of Imogolite by Decylphosphonic Acid Yields an Interface Active Composite Material.

Langmuir 2019 Feb 22. Epub 2019 Feb 22.

The phosphonic acid moiety is commonly used as an anchoring group for the surface modification of imogolite. However, the impact of the reaction on its structure has never been clearly analyzed before. We study the reaction of imogolite and decylphosphonic acid by combining infrared spectroscopy, X-ray scattering, scanning electron microscopy, transmission electron microscopy and solid-state nuclear magnetic resonance spectroscopy. Read More

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http://dx.doi.org/10.1021/acs.langmuir.8b04242DOI Listing
February 2019

A disease-causing intronic point mutation C19G alters tau exon 10 splicing via RNA secondary structure rearrangement.

Biochemistry 2019 Feb 22. Epub 2019 Feb 22.

Alternative splicing of the MAPT cassette exon 10 produces tau isoforms with 4 microtubule-binding repeat domains (4R) upon exon inclusion, or 3 repeats (3R) upon exon skipping. In human neurons, deviations from the physiological 4R:3R ratio of ~1:1 lead to Frontotemporal dementia with Parkinsonism linked to chromosome-17 (FTDP-17). Certain FTDP-17-associated mutations affect a regulatory hairpin that sequesters the exon 10 5' splice site (5'ss, located at the exon 10-intron 10 junction). Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00001DOI Listing
February 2019

Scaffolding kidney organoids on silk.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Maine Medical Center Research Institute, Scarborough, ME.

End stage kidney disease affects hundreds of thousands of patients in the United States. The therapy of choice is kidney replacement, but availability of organs is limited and alternative sources of tissue are needed. Generation of new kidney tissue in the laboratory has been made possible through pluripotent cell reprogramming and directed differentiation. Read More

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http://dx.doi.org/10.1002/term.2830DOI Listing
February 2019

Investigating Nrg1 signaling in the regenerating axolotl spinal cord using multiplexed FISH.

Dev Neurobiol 2019 Feb 22. Epub 2019 Feb 22.

Northeastern University, Department of Biology, 360 Huntington Ave 134 Mugar Hall, Boston, MA, 02115.

Amputation of a salamander tail leads to functional spinal cord regeneration through activation of endogenous stem cells. Identifying the signaling pathways that control cell proliferation in these neural stem cells will help elucidate the mechanisms underlying the salamander's regenerative ability. Here, we show that Neuregulin 1 (Nrg1)/ErbB2 signaling is an important pathway in the regulation of neural stem cell proliferation in the spinal cord of the axolotl salamander (Ambystoma mexicanum). Read More

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http://dx.doi.org/10.1002/dneu.22670DOI Listing
February 2019

Efficacy of pulsed electromagnetic fields and electromagnetic fields tuned to the ion cyclotron resonance frequency of Ca on chondrogenic differentiation.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Microsystems Laboratory, Institute of Microengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Previous studies provide strong evidence for the therapeutic effect of electromagnetic fields (EMFs) on different tissues including cartilage. Diverse exposure parameters applied in scientific reports and the unknown interacting mechanism of EMF with biological systems, make EMF studies challenging. In 1985, Liboff proposed that when magnetic fields are tuned to the cyclotron resonance frequencies of critical ions, the motion of ions through cell membranes is enhanced, and thus biological effects appear. Read More

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http://dx.doi.org/10.1002/term.2829DOI Listing
February 2019

Tumor-derived exosomes: Implication in angiogenesis and antiangiogenesis cancer therapy.

J Cell Physiol 2019 Feb 22. Epub 2019 Feb 22.

Department of Clinical Genetics, Linköping University Hospital, Linköping, Sweden.

Tumor cells utilize different strategies to communicate with neighboring tissues for facilitating tumor progression and invasion, one of these strategies has been shown to be the release of exosomes. Exosomes are small nanovesicles secreted by all kind of cells in the body, especially cancer cells, and mediate cell to cell communications. Exosomes play an important role in cancer invasiveness by harboring various cargoes that could accelerate angiogenesis. Read More

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http://dx.doi.org/10.1002/jcp.28374DOI Listing
February 2019

Ebp1 p48 promotes oncogenic activities in human colon cancer cells through regulation of TIF-90-mediated ribosomal RNA synthesis.

J Cell Physiol 2019 Feb 22. Epub 2019 Feb 22.

Department of Medical Biotechnology, Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam.

The ErbB3-binding protein 1 (Ebp1) has been reported as either an oncogenic regulator or a tumor suppressor in a variety of cancers. Here, we show that Ebp1 p48, a predominant expression isoform, is highly expressed in the majority of human colon tumor cells compared with normal adjacent tissues and its expression is required for the oncogenic activities of these cells. Depletion of Ebp1 expression in primary colon cancer cells inhibits cell proliferation, colony forming, and invasion in vitro as well as tumor formation in vivo and enhances cell sensitivity to irradiation. Read More

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http://dx.doi.org/10.1002/jcp.28385DOI Listing
February 2019

A fibrinous and allogeneic fibroblast-enriched membrane as a biocompatible material can improve diabetic wound healing.

Biomater Sci 2019 Feb 22. Epub 2019 Feb 22.

Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran. and Department of Hematology and Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran.

The application of conventional approaches to diabetic wound regeneration has some limitations. Thus, skin substitutes could be a new therapeutic possibility. In this regard, fibrin scaffolds are promising materials due to their desirable characteristics. Read More

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http://dx.doi.org/10.1039/c8bm01377bDOI Listing
February 2019

Human Lacrimal Drainage System Reconstruction, Recanalization, and Regeneration.

Curr Eye Res 2019 Feb 22:1-12. Epub 2019 Feb 22.

a Institute of Functional and Clinical Anatomy , Friedrich-Alexander-University of Erlangen-Nürnberg , Erlangen , Germany.

Purpose: This review examines the broad contexts of the reconstructive and recanalization strategies of the human lacrimal drainage system.

Materials And Methods: A PubMed search was performed using individually and combination of the terms "lacrimal," "reconstruction", "recanalization," "canaliculus," "canalicular," "stenosis," "obstruction," "block," "drainage," "disorder," "disease," "nasolacrimal duct (NLD)," "endoscopy," "dacryoendoscopy," "trauma," "laceration," "stents," "repair," "tubes," "tear," "eyelid," "Sisler," "trephine," and "trephination". Selections from these lists were the basis of examination of reconstruction and recanalization strategies of multiple lacrimal disorders and their outcomes. Read More

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http://dx.doi.org/10.1080/02713683.2019.1580376DOI Listing
February 2019

The effects of dynamic compressive loading on human mesenchymal stem cell osteogenesis in the stiff layer of a bilayer hydrogel.

J Tissue Eng Regen Med 2019 Feb 22. Epub 2019 Feb 22.

Chemical and Biological Engineering, University of Colorado, Boulder, CO.

Bilayer hydrogels with a soft cartilage-like layer and a stiff bone-like layer embedded with human mesenchymal stem cells (hMSCs) are promising for osteochondral tissue engineering. The goals of this work were to evaluate the effects of dynamic compressive loading (2.5% applied strain, 1 Hz) on osteogenesis in the stiff layer and spatially map local mechanical responses (strain, stress, hydrostatic pressure and fluid velocity). Read More

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http://dx.doi.org/10.1002/term.2827DOI Listing
February 2019

Neutrophils Derived from Genetically Modified Human Induced Pluripotent Stem Cells Circulate and Phagocytose Bacteria In Vivo.

Stem Cells Transl Med 2019 Feb 21. Epub 2019 Feb 21.

Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.

Bacterial and fungal infections are a major cause of morbidity and mortality in neutropenic patients. Donor derived neutrophil transfusions have been used for prophylaxis or treatment for infection in neutropenic patients. However, the short half-life and the limited availability of large numbers of donor derived neutrophils for transfusion remain a significant hurdle in the implementation of neutrophil transfusion therapy. Read More

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http://dx.doi.org/10.1002/sctm.18-0255DOI Listing
February 2019

Presence, localization and morphology of TELOCYTES in developmental gonads of fishes.

J Morphol 2019 Feb 21. Epub 2019 Feb 21.

Department of Morphology, Botucatu Biosciences Institute, State University of São Paulo (UNESP), Botucatu, São Paulo, Brazil.

Telocytes are a new defined type of interstitial cells, considered as a stem cell, with very long and thin cytoplasmic extensions. They are present in the vertebrates, and may participate in tissue remodeling. In fish, during gonadal development, the events that culminate with the germinal epithelium formation are well known. Read More

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http://dx.doi.org/10.1002/jmor.20972DOI Listing
February 2019

Abyssinone V, a prenylated flavonoid isolated from the stem bark of Erythrina melanacantha increases oxidative stress and decreases stress resistance in Caenorhabditis elegans.

J Pharm Pharmacol 2019 Feb 21. Epub 2019 Feb 21.

Institute of Agricultural and Nutritional Sciences, Biofunctionality of Secondary Plant Compounds, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.

Objectives: Recent studies showed that distinct extracts of Erythrina species used in the traditional medicine of sub-Saharan Africa are protective against stress conditions. However, the underlying molecular mechanisms as well as relevant compounds remain unclear.

Methods: We used the model organism Caenorhabditis elegans to investigate compounds isolated from the stem bark of Erythrina melanacantha (abyssinone V (1), abyssinon-4'O-methylether (2), sigmoidin B-4'O-methylether (3), glabranin (4), 8-prenylnaringenin (5), citflavanone (6), exiguaflavanone (7) and homoeriodictyol (8)). Read More

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http://dx.doi.org/10.1111/jphp.13074DOI Listing
February 2019

Cyclin-dependent kinase 8/19 inhibition suppresses osteoclastogenesis by downregulating RANK and promotes osteoblast mineralization and cancellous bone healing.

J Cell Physiol 2019 Feb 21. Epub 2019 Feb 21.

Department of Clinical and Experimental Medicine, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden.

Cyclin-dependent kinase 8 (CDK8) is a mediator complex-associated transcriptional regulator that acts depending on context and cell type. While primarily under investigation as potential cancer therapeutics, some inhibitors of CDK8-and its paralog CDK19-have been reported to affect the osteoblast lineage and bone formation. This study investigated the effects of two selective CDK8/19 inhibitors on osteoclastogenesis and osteoblasts in vitro, and further evaluated how local treatment with a CDK8/19 inhibitor affects cancellous bone healing in rats. Read More

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http://dx.doi.org/10.1002/jcp.28321DOI Listing
February 2019

Outcome of transformed follicular lymphoma worsens according to the timing of transformation and to the number of previous therapies. A retrospective multicenter study on behalf of Fondazione Italiana Linfomi (FIL).

Br J Haematol 2019 Feb 21. Epub 2019 Feb 21.

Haematology, ASST Spedali Civili di Brescia, Brescia, Italy.

Optimal treatment for transformed follicular lymphoma (tFL) is not fully defined. Clinical characteristics and treatments that impact on post-transformation outcome of 176 biopsy-proven tFL were analysed. Transformation occurred at initial diagnosis in 52% (Group 1) and after a FL diagnosis in 48% (Group 2). Read More

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http://dx.doi.org/10.1111/bjh.15816DOI Listing
February 2019

Scaling of phloem hydraulic resistance in stems and leaves of the understory angiosperm shrub Illicium parviflorum.

Am J Bot 2019 Feb 21. Epub 2019 Feb 21.

Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA, 02138, USA.

Premise Of The Study: Recent studies in canopy-dominant trees revealed axial scaling of phloem structure. However, whether this pattern is found in woody plants of the understory, the environment of most angiosperms from the ANA grade (Amborellales-Nymphaeales-Austrobaileyales), is unknown.

Methods: We used seedlings and adult plants of the understory tropical shrub Illicium parviflorum, a member of the lineage Austrobaileyales, to explore the anatomy and physiology of the phloem in their aerial parts, including changes through ontogeny. Read More

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http://dx.doi.org/10.1002/ajb2.1241DOI Listing
February 2019

Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction.

Eur Cell Mater 2019 Feb 22;37:113-133. Epub 2019 Feb 22.

Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Krankenhausstraße 7, A-4010 Linz,

The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. Read More

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http://dx.doi.org/10.22203/eCM.v037a08DOI Listing
February 2019

Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells.

Bull Exp Biol Med 2019 Feb 22. Epub 2019 Feb 22.

National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.

Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis. Using proteomic analysis, we identified transgenic SCF in extracellular vesicles released by AAV-modified mesenchymal stromal cells and detected some proteins specific of extracellular vesicles secreted by transduced cells. Read More

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http://dx.doi.org/10.1007/s10517-019-04387-2DOI Listing
February 2019

Tegavivint and the β-catenin/ALDH Axis in Chemotherapy-Resistant and Metastatic Osteosarcoma.

J Natl Cancer Inst 2019 Feb 21. Epub 2019 Feb 21.

Department of Pediatrics, Texas Children's Cancer and Hematology Center, Baylor College of Medicine, Houston, Texas, USA.

Background: The Wnt/β-catenin pathway is closely associated with osteosarcoma (OS) development and metastatic progression. We investigated the antitumor activity of Tegavivint, a novel β-catenin/transducing β-like protein 1 (TBL1) inhibitor, against OS employing in vitro, ex vivo and in vivo cell line and patient-derived xenograft (PDX) models that recapitulate high risk disease.

Methods: The antitumor efficacy of Tegavivint was evaluated in vitro using established OS and PDX-derived cell lines. Read More

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http://dx.doi.org/10.1093/jnci/djz026DOI Listing
February 2019

Female genital tract chronic graft-versus-host disease: A narrative review.

Hematol Transfus Cell Ther 2019 Jan-Mar;41(1):69-75. Epub 2018 Jul 29.

Fundação Doutor Amaral Carvalho, Jaú, SP, Brazil.

Graft-versus-host disease is one of the main causes of morbidity and mortality in patients submitted to hematopoietic stem cell transplantation. This study reviewed the prevalence of lower female genital tract graft-versus-host disease following hematopoietic stem cell transplantation. A systematic search of the literature for articles published between 1982 and 2015 was performed. Read More

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http://dx.doi.org/10.1016/j.htct.2018.06.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371232PMC

Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue.

Hematol Transfus Cell Ther 2019 Jan-Mar;41(1):7-16. Epub 2018 Jun 19.

Transplant Immunology & Stem Cell Unit, Gleneagles Global Hospitals, Hyderabad, India.

Background: Mesenchymal stem cells have immense potential in stem cell-based therapies, however there is a pre-requisite to develop a curative cell dose. Adipose tissue-derived mesenchymal stem cells are promising mainly due to their potential abundance, immunomodulatory effect and remarkable differentiation potential. Nevertheless, senescence may develop during their expansion due to the incidence of genetic instability. Read More

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http://dx.doi.org/10.1016/j.htct.2018.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371406PMC

Assessing the impact of ABO incompatibility on major allogeneic hematopoietic stem cell transplant outcomes: a prospective, single-center, cohort study.

Hematol Transfus Cell Ther 2019 Jan-Mar;41(1):1-6. Epub 2018 Jul 10.

Hospital das Clínicas da Universidade Federal de Minas Gerais (HC UFMG), Belo Horizonte, MG, Brazil.

Background: ABO blood group incompatibility between donor and recipient is associated with a number of immunohematological complications, but is not considered a major contraindication to allogeneic hematopoietic stem cell transplantation. However, available evidence from the literature seems to be conflicting as to the impact of incompatibility on overall survival, event-free survival, transplant-related mortality, graft-versus-host disease, and time to neutrophil and platelet engraftment.

Methods: This single-center, prospective, cohort study included patients with hematological malignancies who underwent a first allogeneic hematopoietic stem cell transplantation between 2008 and 2014. Read More

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http://dx.doi.org/10.1016/j.htct.2018.05.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371230PMC

STEM faculty who believe ability is fixed have larger racial achievement gaps and inspire less student motivation in their classes.

Sci Adv 2019 Feb 15;5(2):eaau4734. Epub 2019 Feb 15.

Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, USA.

An important goal of the scientific community is broadening the achievement and participation of racial minorities in STEM fields. Yet, professors' beliefs about the fixedness of ability may be an unwitting and overlooked barrier for stigmatized students. Results from a longitudinal university-wide sample (150 STEM professors and more than 15,000 students) revealed that the racial achievement gaps in courses taught by more fixed mindset faculty were twice as large as the achievement gaps in courses taught by more growth mindset faculty. Read More

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http://dx.doi.org/10.1126/sciadv.aau4734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377274PMC
February 2019

Combination of pleuroparenchymal fibroelastosis with non-specific interstitial pneumonia and bronchiolitis obliterans as a complication of hematopoietic stem cell transplantation - Clues to a potential mechanism.

Respir Med Case Rep 2019 4;26:244-247. Epub 2019 Feb 4.

Department of Pathology, Nagasaki University Hospital, Nagasaki, Japan.

Pleuroparenchymal fibroelastosis (PPFE) is a newly described entity of interstitial lung disease, which has been recently recognized as a rare complication of bone marrow transplantation. We report a case of 30-year-old man who developed a unique combination of pleuroparenchymal fibroelastosis with cellular and fibrotic non-specific interstitial pneumonia (NSIP) and bronchiolitis obliterans (BO) sixteen years after hematopoietic stem cell transplantation. Histological examination revealed almost exclusive infiltration of CD3-positive T lymphocytes associated with lymphoepithelial lesions and multi-focal denudation of covering epithelial cells in all components. Read More

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http://dx.doi.org/10.1016/j.rmcr.2019.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370562PMC
February 2019
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