231,567 results match your criteria Stem cell research & therapy[Journal]


Leptomeningeal dissemination: a sinister pattern of medulloblastoma growth.

J Neurosurg Pediatr 2019 Feb 15:1-9. Epub 2019 Feb 15.

3Cancer Research Program, Research Institute of the McGill University Health Center and Department of Surgery, McGill University, Montreal, Quebec, Canada.

Leptomeningeal dissemination (LMD) is the defining pattern of metastasis for medulloblastoma. Although LMD is responsible for virtually 100% of medulloblastoma deaths, it remains the least well-understood part of medulloblastoma pathogenesis. The fact that medulloblastomas rarely metastasize outside the CNS but rather spread almost exclusively to the spinal and intracranial leptomeninges has fostered the long-held belief that medulloblastoma cells spread directly through the CSF, not the bloodstream. Read More

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http://dx.doi.org/10.3171/2018.11.PEDS18506DOI Listing
February 2019

MSC exosomes alleviate temporomandibular joint osteoarthritis by attenuating inflammation and restoring matrix homeostasis.

Biomaterials 2019 Feb 8;200:35-47. Epub 2019 Feb 8.

Faculty of Dentistry, National University of Singapore, Singapore; Tissue Engineering Program, Life Sciences Institute, National University of Singapore, Singapore. Electronic address:

The efficacy of mesenchymal stem cell (MSC) therapies is increasingly attributed to paracrine secretion, particularly exosomes. In this study, we investigated the role of MSC exosomes in the regulation of inflammatory response, nociceptive behaviour, and condylar cartilage and subchondral bone healing in an immunocompetent rat model of temporomandibular joint osteoarthritis (TMJ-OA). We observed that exosome-mediated repair of osteoarthritic TMJs was characterized by early suppression of pain and degeneration with reduced inflammation, followed by sustained proliferation and gradual improvements in matrix expression and subchondral bone architecture, leading to overall joint restoration and regeneration. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.02.006DOI Listing
February 2019

Zscan10 suppresses osteoclast differentiation by regulating expression of Haptoglobin.

Bone 2019 Feb 13. Epub 2019 Feb 13.

Department of Pathophysiology, Graduate School of Medicine, Ehime University, Toon, Ehime, Japan; Division of Laboratory Animal Research, Advanced Research Support Center, Ehime University, Toon, Ehime, Japan; Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, Toon, Ehime, Japan. Electronic address:

Zinc finger and SCAN domain containing 10 (Zscan10) was identified as a novel transcription factor that is involved in osteoclast differentiation in our previous report. However, the biological functions of Zscan10 are not fully understood except its roles in the maintenance of genome stability and pluripotency of embryonic stem cells. Therefore, the purpose of this study was to clarify the function of Zscan10 in somatic cells, especially during osteoclast differentiation. Read More

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http://dx.doi.org/10.1016/j.bone.2019.02.011DOI Listing
February 2019

CXCL11 promotes self-renewal and tumorigenicity of α2δ1 liver tumor-initiating cells through CXCL11/CXCR3/ERK1/2 signaling.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Cell Biology, Peking University Cancer Hospital and Institute, 52 Fucheng Road, Beijing, 100142, China. Electronic address:

Tumor-initiating cells (TICs), which are responsible for sustaining tumor growth and recurrence, rely on several regulatory factors. However, the mechanism of inflammation-related molecules in the acquisition and maintenance of TIC properties in hepatocellular carcinoma (HCC) remains elusive. We previously demonstrated that the voltage-gated calcium channel α2δ1 subunit is a functional surface marker of HCC TICs. Read More

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http://dx.doi.org/10.1016/j.canlet.2019.02.016DOI Listing
February 2019

Aegle marmelos leaf extract ameliorates the cognitive impairment and oxidative stress induced by intracerebroventricular streptozotocin in male rats.

Life Sci 2019 Feb 13. Epub 2019 Feb 13.

Amity Institute of Molecular Medicine & Stem Cell Research, Amity University, Sector-125, Noida 201313, India. Electronic address:

Aims: Aegle marmelos (L.) Correa (A. marmelos) has been used in Ayurvedic medicine as a brain tonic however its neuroprotective effect against streptozotocin (STZ) induced cognitive impairment and oxidative stress has not been reported yet in vivo. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.02.032DOI Listing
February 2019

Strategies for controlling egress of therapeutic cells from hydrogel microcapsules.

J Tissue Eng Regen Med 2019 Feb 16. Epub 2019 Feb 16.

Department of Physics, University of Ottawa, Ottawa, Canada.

Endothelial progenitor cells (EPCs) and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focused on cell-encapsulation, to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules. Read More

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http://dx.doi.org/10.1002/term.2818DOI Listing
February 2019

Mitochondria-centric bioenergetic characteristics in cancer stem-like cells.

Arch Pharm Res 2019 Feb 15. Epub 2019 Feb 15.

Department of Surgery, Yonsei University Health System, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Korea.

Metabolic and genotoxic stresses that arise during tumor progression and anti-cancer treatment, respectively, can impose a selective pressure to promote cancer evolution in the tumor microenvironment. This process ultimately selects for the most "fit" clones, which generally have a cancer stem cell like phenotype with features of drug resistance, epithelial-mesenchymal transition, invasiveness, and high metastatic potential. From a bioenergetics perspective, these cancer stem-like cells (CSCs) exhibit mitochondria-centric energy metabolism and are capable of opportunistically utilizing available nutrients such as fatty acids to generate ATP and other metabolic substances, providing a selective advantage for their survival in an impermissible environment and metabolic context. Read More

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http://dx.doi.org/10.1007/s12272-019-01127-yDOI Listing
February 2019

Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.

Methods Mol Biol 2019 Feb 16. Epub 2019 Feb 16.

Cell Biology Group, Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED), Barcelona, Spain.

The maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Read More

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http://dx.doi.org/10.1007/7651_2019_210DOI Listing
February 2019

Stem Cells from Human Extracted Deciduous Teeth Expanded in Foetal Bovine and Human Sera Express Different Paracrine Factors After Exposure to Freshly Prepared Human Serum.

Adv Exp Med Biol 2019 Feb 16. Epub 2019 Feb 16.

Regenerative Dentistry Research Group, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.

Background: The response of stem cells to paracrine factors within the host's body plays an important role in the regeneration process after transplantation. The aim of this study was to determine the viability and paracrine factor profile of stem cells from human extracted deciduous teeth (SHED) pre-cultivated in media supplemented with either foetal bovine serum (FBS) or pooled human serum (pHS) in the presence of individual human sera (iHS).

Methods: SHED (n = 3) from passage 4 were expanded in FBS (FBS-SHED) or pHS (pHS-SHED) supplemented media until passage 7. Read More

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http://dx.doi.org/10.1007/5584_2018_299DOI Listing
February 2019

Evidence for personalised medicine: mechanisms, correlation, and new kinds of black box.

Theor Med Bioeth 2019 Feb 15. Epub 2019 Feb 15.

Macquarie University, North Ryde, NSW, Australia.

Personalised medicine (PM) has been discussed as a medical paradigm shift that will improve health while reducing inefficiency and waste. At the same time, it raises new practical, regulatory, and ethical challenges. In this paper, we examine PM strategies epistemologically in order to develop capacities to address these challenges, focusing on a recently proposed strategy for developing patient-specific models from induced pluripotent stem cells (iPSCs) so as to make individualised treatment predictions. Read More

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http://dx.doi.org/10.1007/s11017-019-09482-zDOI Listing
February 2019

SCMCIE94: an intensified pilot treatment protocol known to be associated with cure in CD 56-negative non-pelvic isolated Ewing sarcoma (EWS) is also associated with no early relapses in non-metastatic extremity EWS.

Cancer Chemother Pharmacol 2019 Feb 15. Epub 2019 Feb 15.

The Rina Zaizov Hematology-Oncology Division, Schneider Children's Medical Center of Israel, Petach Tikva, Israel.

Purpose: We report the unexpected absence of early relapse (before 30 months) in 24 consecutive patients with isolated limb primary Ewing sarcoma treated with an intensified pilot protocol, SCMCIE94.

Methods: Clinical data for the study were collected retrospectively from the patient files. The protocol included 6 courses of chemotherapy, split radiation, and limb salvage surgery. Read More

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http://dx.doi.org/10.1007/s00280-019-03789-3DOI Listing
February 2019

A functional subset of CD8 T cells during chronic exhaustion is defined by SIRPα expression.

Nat Commun 2019 Feb 15;10(1):794. Epub 2019 Feb 15.

Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, MT, 59840, USA.

Prolonged exposure of CD8 T cells to antigenic stimulation, as in chronic viral infections, leads to a state of diminished function termed exhaustion. We now demonstrate that even during exhaustion there is a subset of functional CD8 T cells defined by surface expression of SIRPα, a protein not previously reported on lymphocytes. On SIRPα CD8 T cells, expression of co-inhibitory receptors is counterbalanced by expression of co-stimulatory receptors and it is only SIRPα cells that actively proliferate, transcribe IFNγ and show cytolytic activity. Read More

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http://dx.doi.org/10.1038/s41467-019-08637-9DOI Listing
February 2019

Fhit-Fdxr interaction in the mitochondria: modulation of reactive oxygen species generation and apoptosis in cancer cells.

Cell Death Dis 2019 Feb 15;10(3):147. Epub 2019 Feb 15.

Department of Cancer Biology and Genetics, The Ohio State University Comprehensive Cancer Center, Columbus, OH, 43210, USA.

Fhit protein is lost in cancers of most, perhaps all, cancer types; when restored, it can induce apoptosis and suppress tumorigenicity, as shown in vitro and in mouse tumor models in vivo. Following protein cross-linking and proteomics analyses, we characterized a Fhit protein complex involved in triggering Fhit-mediated apoptosis. The complex includes the heat-shock chaperonin pair, HSP60/10, which is likely involved in importing Fhit into the mitochondria, where it interacts with ferredoxin reductase, responsible for transferring electrons from NADPH to cytochrome P450 via ferredoxin, in electron transport chain complex III. Read More

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http://dx.doi.org/10.1038/s41419-019-1414-7DOI Listing
February 2019

Platelet-rich plasma improves therapeutic effects of menstrual blood-derived stromal cells in rat model of intrauterine adhesion.

Stem Cell Res Ther 2019 Feb 15;10(1):61. Epub 2019 Feb 15.

Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodeling of Liaoning Province, Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital affiliated to China Medical University, No. 39 Huaxiang Road, Tiexi District, Shenyang, Liaoning, China.

Background: Intrauterine adhesion (IUA) is a major cause of female secondary infertility. We previously demonstrated that menstrual blood-derived stromal cell (MenSC) transplantation helped severe IUA patients have pregnancy and endometrium regeneration. We also initiated platelet-rich plasma (PRP) acted as a beneficial supplement in MenSC culturing and a potential endometrial receptivity regulator. Read More

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http://dx.doi.org/10.1186/s13287-019-1155-7DOI Listing
February 2019

8-chloro-adenosine activity in FLT3-ITD acute myeloid leukemia.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Hematology Malignancies and Stem Cell Transplantation Institute, Gehr Family Center for Leukemia Research, City of Hope National Medical Center, Duarte, California.

Nucleoside analogs represent the backbone of several distinct chemotherapy regimens for acute myeloid leukemia (AML) and combination with tyrosine kinase inhibitors has improved survival of AML patients, including those harboring the poor-risk FLT3-ITD mutation. Although these compounds are effective in killing proliferating blasts, they lack activity against quiescent leukemia stem cells (LSCs), which contributes to initial treatment refractoriness or subsequent disease relapse. The reagent 8-chloro-adenosine (8-Cl-Ado) is a ribose-containing, RNA-directed nucleoside analog that is incorporated into newly transcribed RNA rather than in DNA, causing inhibition of RNA transcription. Read More

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http://dx.doi.org/10.1002/jcp.28294DOI Listing
February 2019

The roles of signaling pathways in liver repair and regeneration.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

The liver has remarkable regeneration potency that restores liver mass and sustains body hemostasis. Liver regeneration through signaling pathways following resection or moderate damages are well studied. Various cell signaling, growth factors, cytokines, receptors, and cell types implicated in liver regeneration undergo controlled hypertrophy and proliferation. Read More

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http://dx.doi.org/10.1002/jcp.28336DOI Listing
February 2019

Use of stem cells as carriers of oncolytic viruses for cancer treatment.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Therapeutic application of stem cells and oncolytic viruses in cancer treatment has rapidly increased in the last decade. Oncolytic viruses are considered as a new class of anticancer agents because of their ability to selectively infect and destroy cancer cells. Furthermore, regarding the specific migratory capacity of stem cells, they can be used as carriers or vectors targeting metastatic cancer. Read More

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http://dx.doi.org/10.1002/jcp.28320DOI Listing
February 2019

Meeting report - Building the Cell 2018.

J Cell Sci 2019 Feb 15;132(5). Epub 2019 Feb 15.

Centre for Stem Cells & Regenerative Medicine, King's College London, London SE1 9RT, UK

Cell biologists from all around the world gathered in Paris on the 26 to 28 September 2018 to participate in the 3rd international meeting 'Building the Cell'. It was organized by Hélène Barelli, Arnaud Echard, Thierry Galli, Florence Niedergang, Manuel Théry and Marie Hélène Verlhac on behalf of the French Society for Cell Biology (SBCF) at the Institut Pasteur. Around 230 participants joined the meeting for stimulating talks, discussions, poster sessions, and a gala dinner on the Seine that included a music performance by the rock group 'Membrane Band'. Read More

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http://dx.doi.org/10.1242/jcs.229765DOI Listing
February 2019

Motor neuron disease-associated loss of nuclear TDP-43 is linked to DNA double-strand break repair defects.

Proc Natl Acad Sci U S A 2019 Feb 15. Epub 2019 Feb 15.

Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX 77030;

Genome damage and their defective repair have been etiologically linked to degenerating neurons in many subtypes of amyotrophic lateral sclerosis (ALS) patients; however, the specific mechanisms remain enigmatic. The majority of sporadic ALS patients feature abnormalities in the transactivation response DNA-binding protein of 43 kDa (TDP-43), whose nucleo-cytoplasmic mislocalization is characteristically observed in spinal motor neurons. While emerging evidence suggests involvement of other RNA/DNA binding proteins, like FUS in DNA damage response (DDR), the role of TDP-43 in DDR has not been investigated. Read More

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http://dx.doi.org/10.1073/pnas.1818415116DOI Listing
February 2019

Voices: The Importance of Fetal Tissue Research.

Authors:

Cell Stem Cell 2019 Feb 5. Epub 2019 Feb 5.

The value of fetal tissue research cannot be understated and has led to remarkable advances in understanding human development, therapeutic discovery, and stem cell research across many organ systems. We asked 9 researchers to reflect on the impact and key contributions of fetal tissue in their field of expertise. Read More

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http://dx.doi.org/10.1016/j.stem.2019.01.012DOI Listing
February 2019

Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex.

Neuron 2019 Feb 7. Epub 2019 Feb 7.

Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Neurology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address:

In the developing human neocortex, progenitor cells generate diverse cell types prenatally. Progenitor cells and newborn neurons respond to signaling cues, including neurotransmitters. While single-cell RNA sequencing has revealed cellular diversity, physiological heterogeneity has yet to be mapped onto these developing and diverse cell types. Read More

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http://dx.doi.org/10.1016/j.neuron.2019.01.027DOI Listing
February 2019

Bioactivity Determination of a Therapeutic Recombinant Human Keratinocyte Growth Factor by a Validated Cell-based Bioassay.

Molecules 2019 Feb 15;24(4). Epub 2019 Feb 15.

National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing 100050, China.

The therapeutic recombinant human keratinocyte growth factor 1 (rhKGF-1) was approved by the FDA for oral mucositis resulting from hematopoietic stem cell transplantation for hematological malignancies in 2004. However, no recommended bioassay for rhKGF-1 bioactivity has been recorded in the U.S. Read More

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http://dx.doi.org/10.3390/molecules24040699DOI Listing
February 2019

The Contribution of Pluripotent Stem Cell (PSC)-Based Models to the Study of Fragile X Syndrome (FXS).

Brain Sci 2019 Feb 15;9(2). Epub 2019 Feb 15.

Stem Cell Research Laboratory, Medical Genetics Institute, Shaare Zedek Medical Center, Jerusalem 91031, Israel.

Fragile X syndrome (FXS) is the most common heritable form of cognitive impairment. It results from a deficiency in the fragile X mental retardation protein (FMRP) due to a CGG repeat expansion in the 5'-UTR of the X-linked gene. When CGGs expand beyond 200 copies, they lead to epigenetic gene silencing of the gene. Read More

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http://dx.doi.org/10.3390/brainsci9020042DOI Listing
February 2019

Current Therapeutic Results and Treatment Options for Older Patients with Relapsed Acute Myeloid Leukemia.

Cancers (Basel) 2019 Feb 14;11(2). Epub 2019 Feb 14.

Division of Hematology, Spedali Civili, 25123 Brescia, Italy.

Considerable progress has been made in the treatment of acute myeloid leukemia (AML). However, current therapeutic results are still unsatisfactory in untreated high-risk patients and poorer in those with primary refractory or relapsed disease. In older patients, reluctance by clinicians to treat unfit patients, higher AML cell resistance related to more frequent adverse karyotype and/or precedent myelodysplastic syndrome, and preferential involvement of chemorefractory early hemopoietic precursors in the pathogenesis of the disease further account for poor prognosis, with median survival lower than six months. Read More

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http://dx.doi.org/10.3390/cancers11020224DOI Listing
February 2019

Stem Cell-Derived Extracellular Vesicles for Treating Joint Injury and Osteoarthritis.

Nanomaterials (Basel) 2019 Feb 14;9(2). Epub 2019 Feb 14.

Raymond Purves Bone and Joint Research Laboratories, Institute of Bone and Joint Research, Kolling Institute, Northern Sydney Local Health District, Faculty of Medicine and Health, University of Sydney, St Leonards, NSW 2065, Australia.

Extracellular vesicles (EVs) are nanoscale particles secreted by almost all cell types to facilitate intercellular communication. Stem cell-derived EVs theoretically have the same biological functions as stem cells, but offer the advantages of small size, low immunogenicity, and removal of issues such as low cell survival and unpredictable long-term behaviour associated with direct cell transplantation. They have been an area of intense interest in regenerative medicine, due to the potential to harness their anti-inflammatory and pro-regenerative effects to induce healing in a wide variety of tissues. Read More

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http://dx.doi.org/10.3390/nano9020261DOI Listing
February 2019

Unveiling Mesenchymal Stromal Cells' Organizing Function in Regeneration.

Int J Mol Sci 2019 Feb 14;20(4). Epub 2019 Feb 14.

Institute for Regenerative Medicine, Medical research and education center, Lomonosov Moscow State University, Moscow 119192, Russia.

Regeneration is a fundamental process attributed to the functions of adult stem cells. In the last decades, delivery of suspended adult stem cells is widely adopted in regenerative medicine as a leading means of cell therapy. However, adult stem cells cannot complete the task of human body regeneration effectively by themselves as far as they need a receptive microenvironment (the niche) to engraft and perform properly. Read More

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http://dx.doi.org/10.3390/ijms20040823DOI Listing
February 2019

Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles.

Cancers (Basel) 2019 Jan 30;11(2). Epub 2019 Jan 30.

School of Medical and Health Sciences, Edith Cowan University, Perth, WA 6027, Australia.

Cutaneous melanoma circulating tumour cells (CTCs) are phenotypically and molecularly heterogeneous. We profiled the gene expression of CTC subpopulations immunomagnetic-captured by targeting either the melanoma-associated marker, MCSP, or the melanoma-initiating marker, ABCB5. Firstly, the expression of a subset of melanoma genes was investigated by RT-PCR in MCSP-enriched and ABCB5-enriched CTCs isolated from a total of 59 blood draws from 39 melanoma cases. Read More

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http://dx.doi.org/10.3390/cancers11020157DOI Listing
January 2019

Soybean Stem Lignin Concentration Relates to Resistance to Sclerotinia sclerotiorum.

Plant Dis 2009 Feb;93(2):149-154

Department of Plant Pathology, University of Wisconsin - Madison; 1630 Linden Dr., Madison, WI 53706.

Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is an economically important disease of soybean (Glycine max) in the north-central United States and other temperate regions throughout the world. The occurrence and severity of Sclerotinia stem rot in the field is highly dependent upon prevailing environmental conditions, which can prove problematic when evaluating soybean accessions for resistance. The identification of an environmentally stable plant trait associated with resistance to S. Read More

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http://dx.doi.org/10.1094/PDIS-93-2-0149DOI Listing
February 2009

Generation of two isogenic iPSC lines with either a heterozygous or a homozygous E280A mutation in the PSEN1 gene.

Stem Cell Res 2019 Feb 7;35:101403. Epub 2019 Feb 7.

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark. Electronic address:

Alzheimer's disease (AD) is the most common form of dementia. Mutations in the gene PSEN1 encoding Presenilin1 are known to cause familial forms of AD with early age of onset. The most common mutation in the PSEN1 gene is the E280A mutation. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101403DOI Listing
February 2019

Assessment of PCL/carbon material scaffolds for bone regeneration.

J Mech Behav Biomed Mater 2019 Jan 31;93:52-60. Epub 2019 Jan 31.

School of Mechanical, Aerospace and Civil Engineering, The University of Manchester, Manchester M13 9PL, UK. Electronic address:

Biomanufacturing is a relatively new research domain focusing on the use of additive manufacturing technologies, biomaterials, cells and biomolecular signals to produce tissue constructs for tissue engineering. For bone regeneration, researchers are focusing on the use of polymeric and polymer/ceramic scaffolds seeded with osteoblasts or mesenchymal stem cells. However, the design of high-performance scaffolds in terms of mechanical, cell-stimulation and biological performance is still required. Read More

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http://dx.doi.org/10.1016/j.jmbbm.2019.01.020DOI Listing
January 2019

Access to Hematopoietic Stem Cell Transplantation among Pediatric Patients with Acute Lymphoblastic Leukemia: A Population-based Analysis.

Biol Blood Marrow Transplant 2019 Feb 12. Epub 2019 Feb 12.

Division of Hematology/Oncology, Hospital for Sick Children, Toronto, Ontario.

Introduction: Access to hematopoietic stem cell transplantation (HSCT) in pediatric acute lymphoblastic leukemia (ALL) is primarily dependent on disease-related factors but may be influenced by social and economic determinants.

Methods: We included all children < 15 years old with newly diagnosed ALL in Canada between 2001 and 2018 using the Cancer in Young People in Canada (CYP-C) national registry. We examined factors potentially associated with the likelihood of receiving HSCT using univariate and multivariable logistic regression models. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10838791193013
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http://dx.doi.org/10.1016/j.bbmt.2019.02.009DOI Listing
February 2019
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miR-3099 promotes neurogenesis and inhibits astrogliogenesis during murine neural development.

Gene 2019 Feb 12. Epub 2019 Feb 12.

Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address:

MicroRNA-3099 is highly expressed during neuronal differentiation and development of the central nervous system. Here we characterised the role of miR-3099 during neural differentiation and embryonic brain development using a stable and regulatable mouse embryonic stem cell culture system for miR-3099 expression and in utero electroporation of miR-3099 expression construct into E15.5 embryonic mouse brains. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.014DOI Listing
February 2019

Stem extract of Tabebuia chrysantha induces apoptosis by targeting sEGFR in Ehrlich Ascites Carcinoma.

J Ethnopharmacol 2019 Feb 12. Epub 2019 Feb 12.

Pharmaceutical Analysis Laboratory, SIMS College of Pharmacy, Guntur, India. Electronic address:

Ethnopharmacological Relevance: The plant Tabebuia chrysantha (Jaq.) Nicholson (Bignoniaceae) is commonly known as "Golden Goddess" in the Southern part of India and "Golden Trumpet Tree " in Central America. Stems of this plant have been traditionally used for antioxidant, anti-inflammatory, antimicrobial and anticancer actions. Read More

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http://dx.doi.org/10.1016/j.jep.2019.02.023DOI Listing
February 2019

Special issue on pluripotent stem cells and hematopoiesis.

Exp Hematol 2019 Feb 12. Epub 2019 Feb 12.

Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA; Division of Pediatric Hematology-Oncology, Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, CA; Broad Stem Cell Research Center, University of California, Los Angeles, CA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA. Electronic address:

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http://dx.doi.org/10.1016/j.exphem.2019.02.001DOI Listing
February 2019

Collagen XIII and other ECM components in the assembly and disease of the neuromuscular junction.

Anat Rec (Hoboken) 2019 Feb 15. Epub 2019 Feb 15.

Oulu Center for Cell-Matrix Research, Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, P.O. Box 5400, 90014 University of Oulu, Oulu, Finland.

Alongside playing structural roles, the extracellular matrix (ECM) acts as an interaction platform for cellular homeostasis, organ development, and maintenance. The necessity of the ECM is highlighted by the diverse, sometimes very serious diseases that stem from defects in its components. The neuromuscular junction (NMJ) is a large peripheral motor synapse differing from its central counterparts through the ECM included at the synaptic cleft. Read More

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http://dx.doi.org/10.1002/ar.24092DOI Listing
February 2019

Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions.

Stem Cells Transl Med 2019 Feb 15. Epub 2019 Feb 15.

Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, USA.

Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e. Read More

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http://dx.doi.org/10.1002/sctm.18-0189DOI Listing
February 2019

Development and characterization of novel clinical grade neonatal porcine bone marrow-derived mesenchymal stem cells.

Xenotransplantation 2019 Feb 15:e12501. Epub 2019 Feb 15.

Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Japan.

Due to recent advances in research on mesenchymal stem cells (MSCs), MSCs are expected to be used in various clinical applications. However, securing adequate cadaveric donors and safety of living donors are major issues. To solve such issues, we have examined to develop clinical grade neonatal porcine bone marrow-derived MSCs (npBM-MSCs). Read More

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http://dx.doi.org/10.1111/xen.12501DOI Listing
February 2019

Survival and functional outcomes of molecularly defined childhood posterior fossa ependymoma: Cure at a cost.

Cancer 2019 Feb 15. Epub 2019 Feb 15.

Division of Haematology/Oncology, Hospital for Sick Children, Toronto, Ontario, Canada.

Background: Posterior fossa ependymoma (PFE) comprises 2 groups, PF group A (PFA) and PF group B (PFB), with stark differences in outcome. However, to the authors' knowledge, the long-term outcomes of PFA ependymoma have not been described fully. The objective of the current study was to identify predictors of survival and neurocognitive outcome in a large consecutive cohort of subgrouped patients with PFE over 30 years. Read More

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http://dx.doi.org/10.1002/cncr.31995DOI Listing
February 2019

N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide promotes bone formation via the canonical Wnt/β-catenin signaling pathway.

Phytother Res 2019 Feb 15. Epub 2019 Feb 15.

Key Laboratory of Mental Health of the Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.

Osteoporosis is characterized by low bone mineral density and microarchitectural deterioration of bone tissue. N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide (MBOC) is one of the macamides isolated from Maca (Lepidium meyenii Walp.), a cruciferous plant from the Andes of Peru. Read More

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http://dx.doi.org/10.1002/ptr.6301DOI Listing
February 2019

Platelet-rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF-β/SMAD signaling pathway.

J Cell Physiol 2019 Feb 15. Epub 2019 Feb 15.

Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Life Sciences Institute, Guangxi Medical University, Nanning, People's Republic of China.

The tissue engineering technique using mesenchymal stem cells (MSCs) and scaffolds is promising. Transforming growth factor-β1 (TGF-β1) is generally accepted as an chondrogenic agent, but immunorejection and unexpected side effects, such as tumorigenesis and heterogeneity, limit its clinical application. Autogenous platelet-rich plasma (PRP), marked by low immunogenicity, easy accessibility, and low-cost, may be favorable for cartilage regeneration. Read More

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http://dx.doi.org/10.1002/jcp.28211DOI Listing
February 2019

The rs61742690 (S783N) single nucleotide polymorphism is a suitable target for disrupting BCL11A-mediated foetal-to-adult globin switching.

PLoS One 2019 15;14(2):e0212492. Epub 2019 Feb 15.

Department of Genetic Research, Institute for Research and Medical Consultation (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Background: B-cell lymphoma/leukaemia 11A (BCL11A) is a C2H2-type zinc-finger transcription factor protein that is a critical modulator of haemoglobin switching and suppresses the production of foetal haemoglobin. Variation in the BCL11A gene ameliorates the severity of sickle cell disease (SCD) and β-thalassemia (β-thal). The BCL11A gene is located on chromosome 2p16. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212492PLOS
February 2019

Spatiotemporal Expression of Three Secretoglobin Proteins, SCGB1A1, SCGB3A1, and SCGB3A2, in Mouse Airway Epithelia.

J Histochem Cytochem 2019 Feb 15:22155419829050. Epub 2019 Feb 15.

Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

Secretoglobins (SCGBs) are cytokine-like small molecular weight secreted proteins with largely unknown biological functions. Three SCGB proteins, SCGB1A1, SCGB3A1, and SCGB3A2, are predominantly expressed in lung airways. To gain insight into the possible functional relationships among the SCGBs, their protein and mRNA expression patterns were examined in lungs during gestation and in adult mice, using Scgb3a1-null and Scgb3a2-null mice as negative controls, by immunohistochemistry and by qRT-PCR analysis, respectively. Read More

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http://dx.doi.org/10.1369/0022155419829050DOI Listing
February 2019

iPSC- and mesenchymoangioblast-derived mesenchymal stem cells provide greater protection against experimental chronic allergic airways disease compared with a clinically used corticosteroid.

FASEB J 2019 Feb 15:fj201802307R. Epub 2019 Feb 15.

Monash Biomedicine Discovery Institute Monash University, Clayton, Victoria, Australia.

The airway remodeling (AWR) associated with chronic allergic airways disease (AAD)/asthma contributes to irreversible airway obstruction. This study compared and combined the antiremodeling and other effects of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) with the corticosteroid dexamethasone (Dex) in experimental chronic AAD/asthma. Female BALB/c mice subjected to 11 wk of ovalbumin (Ova)-induced chronic AAD were intranasally administered MCA-MSCs (1 × 10 cells/mouse; once weekly on wk 10 and 11), Dex (0. Read More

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http://dx.doi.org/10.1096/fj.201802307RDOI Listing
February 2019

Bioenergetic deficits in Huntington's disease iPSC-derived neural cells and rescue with glycolytic metabolites.

Hum Mol Genet 2019 Feb 15. Epub 2019 Feb 15.

Division of Neurobiology, Departments of Psychiatry, Neurology, Pharmacology, and Neuroscience, Johns Hopkins University School of Medicine, 600 North Wolfe Street, CMSC 8-121, Baltimore, Maryland 21287 USA.

Altered cellular metabolism is believed to be an important contributor to pathogenesis of the neurodegenerative disorder Huntington's disease (HD). Research has primarily focused on mitochondrial toxicity, which can cause death of the vulnerable striatal neurons, but other aspects of metabolism have also been implicated. Most previous studies have been carried out using post-mortem human brain or non-human cells. Read More

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http://dx.doi.org/10.1093/hmg/ddy430DOI Listing
February 2019
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Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states.

Epigenetics Chromatin 2019 Feb 15;12(1):14. Epub 2019 Feb 15.

Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University, 6500 HB, Nijmegen, The Netherlands.

Background: Genomic imprinting, resulting in parent-of-origin specific gene expression, plays a critical role in mammalian development. Here, we apply allele-specific RNA-seq on isogenic B6D2F1 mice to assay imprinted genes in tissues from early embryonic tissues between E3.5 and E7. Read More

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http://dx.doi.org/10.1186/s13072-019-0259-8DOI Listing
February 2019

microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9.

Stem Cell Res Ther 2019 Feb 15;10(1):59. Epub 2019 Feb 15.

Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, China.

Background: The regulatory mechanism of insulin-producing cells (IPCs) differentiation from induced pluripotent stem cells (iPSCs) in vitro is very important in the phylogenetics of pancreatic islets, the molecular pathogenesis of diabetes, and the acquisition of high-quality pancreatic β-cells derived from stem cells for cell therapy.

Methods: miPSCs were induced for IPCs differentiation. miRNA microarray assays were performed by using total RNA from our iPCs-derived IPCs containing undifferentiated iPSCs and iPSCs-derived IPCSs at day 4, day 14, and day 21 during step 3 to screen the differentially expressed miRNAs (DEmiRNAs) related to IPCs differentiation, and putative target genes of DEmiRNAs were predicted by bioinformatics analysis. Read More

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http://dx.doi.org/10.1186/s13287-019-1154-8DOI Listing
February 2019

Induction of mouse peritoneum mesenchymal stem cells into germ cell-like cells using follicular fluid and cumulus cells conditioned media.

Stem Cells Dev 2019 Feb 15. Epub 2019 Feb 15.

Royan Institute, 48499, Embryology, Tehran, Iran (the Islamic Republic of) ;

The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell like cells using human follicular fluid (HFF) and human cumulus conditioned medium (HCCM). Anterior abdominal wall and intestinal peritoneum explants were used for cells isolation and cultured in DMEM. Read More

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http://dx.doi.org/10.1089/scd.2018.0149DOI Listing
February 2019

In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads.

Stem Cells Transl Med 2019 Feb 14. Epub 2019 Feb 14.

Biotechnology and Radiobiology Laboratory, Hadassah - Hebrew University Medical Center, Sharett Institute of Oncology, Jerusalem, Israel.

Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. Read More

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http://dx.doi.org/10.1002/sctm.18-0129DOI Listing
February 2019

Rap1 signal modulators control the maintenance of hematopoietic progenitors in bone marrow and adult long-term hematopoiesis.

Cancer Sci 2019 Feb 15. Epub 2019 Feb 15.

Medical Innovation Center, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.

Adult long-term hematopoiesis depends on sustaining hematopoietic stem/progenitor cells (HSPCs) in bone marrow (BM) niches, where their balance of quiescence, self-renewal, and hematopoietic differentiation is tightly regulated. While various BM stroma cells that produce niche factors have been identified, regulation of the intrinsic responsiveness of HSPCs to the niche factors remains elusive. We previously reported that mice deficient for Sipa1, a Rap1 GTPase-activating protein, develop diverse hematopoietic disorders of late onset. Read More

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http://dx.doi.org/10.1111/cas.13974DOI Listing
February 2019