235,518 results match your criteria Stem cell research & therapy[Journal]


Generation of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p.L43_A44delLA in the SMPD1 gene.

Stem Cell Res 2019 Apr 12;37:101436. Epub 2019 Apr 12.

National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA. Electronic address:

Niemann-Pick disease type B (NPB) is a rare autosomal recessive lysosomal storage disease caused by mutations in the SMPD1 gene, which encodes for acid sphingomyelinase. A human induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts of a 1-year old male patient with NPB that has a heterozygous mutation of a p.L43_A44delLA of SMPD1 using non-integrating Sendai virus technique. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101436DOI Listing

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation.

Stem Cell Res 2019 Apr 13;37:101437. Epub 2019 Apr 13.

Coronary Care Unit, Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Molecular Medicine, Unit of Cardiology, Università degli studi di Pavia, Pavia, Italy; Department of Medicine, University of Cape Town, Cape Town, South Africa. Electronic address:

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18735061193006
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http://dx.doi.org/10.1016/j.scr.2019.101437DOI Listing
April 2019
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Stathmin regulates the proliferation and odontoblastic/ osteogenic differentiation of human dental pulp stem cells through Wnt/β-catenin signaling pathway.

J Proteomics 2019 Apr 19. Epub 2019 Apr 19.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, PR China; College of Stomatology, Southern Medical University, Guangzhou, PR China. Electronic address:

Osteogenic/ odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is a key factor in tooth and pulp regeneration, but its mechanism still remains unknown. The purpose of this research is to look into the mechanism by which Stathmin affects the proliferation and odontoblastic/ osteogenic differentiation of hDPSCs, and whether the Wnt/β- catenin is related to this regulation. First, the Stathmin expression was inhibited by lentiviral vector, after that the transcriptome sequencing technology was used to screen the differentially expressed genes, Wnt5a which related to the regulation of Wnt/β-catenin was regulated. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.014DOI Listing

Metabolic and epigenetic reprogramming in the arsenic-induced cancer stem cells.

Semin Cancer Biol 2019 Apr 19. Epub 2019 Apr 19.

Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI, 48201, USA. Electronic address:

At present, the belief that genetic mutations control every aspect of tumorigenesis is still very popular. Even for the highly debated "bad luck" theory of cancers, it ascertained that random mutation of genes during the self-renewal of somatic stem cells is responsible for cancer initiation. Logically, most of the new therapeutic strategies so far, from molecular targeting to precision medicine or personalized medicine, are genome-obsessed and focused on identifying and targeting these mutated genes. Read More

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http://dx.doi.org/10.1016/j.semcancer.2019.04.003DOI Listing

Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation.

PLoS One 2019 22;14(4):e0215678. Epub 2019 Apr 22.

Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Mesenchymal stem cells (MSCs) are of special interest due their potential clinical use in cell-based therapy. Therapies engaging MSCs are showing increasing promise in the cancer treatment and anticancer drug screening applications. A multitude of growth factors and cytokines secreted from these cells are known to give such multifunctional properties, but details of their role are yet to be absolutely demonstrated. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215678PLOS

Biological responses of human bone mesenchymal stem cells to Ti and TiZr implant materials.

Clin Implant Dent Relat Res 2019 Apr 22. Epub 2019 Apr 22.

Department of Implant Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai JiaoTong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.

Background: Titanium-zirconium alloy (TiZr1317) is a new material used for biological implants. There are several studies on the effects of TiZr implants on the biological characteristics of human bone mesenchymal stem cells (hBMSCs).

Purpose: The purpose of this study was to investigate the biological responses of hBMSCs to implant holes affected by the physicochemical properties of oral implants (Ti , Ti , TiZr , and TiZr ). Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cid.12756
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http://dx.doi.org/10.1111/cid.12756DOI Listing
April 2019
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Tissue engineering approaches for treating pelvic organ prolapse using a novel source of stem/stromal cells and new materials.

Curr Opin Urol 2019 Apr 16. Epub 2019 Apr 16.

The Ritchie Centre and Centre for Reproductive Health, Hudson institute of Medical Research.

Purpose Of Review: Nondegradable transvaginal polypropylene meshes for treating pelvic organ prolapse (POP) are now generally unavailable or banned. In this review, we summarize recent developments using tissue engineering approaches combining alternate degradable scaffolds with a novel source of mesenchymal stem/stromal cells from human endometrium mesenchymal stem cell (eMSC).

Recent Findings: Tissue engineering constructs comprising immunomodulatory, reparative eMSC and biomimetic materials with nanoarchitecture are a promising approach for vaginal repair and improving outcomes of POP surgery. Read More

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http://dx.doi.org/10.1097/MOU.0000000000000634DOI Listing

Stem-cell regenerative medicine as applied to the penis.

Curr Opin Urol 2019 Apr 16. Epub 2019 Apr 16.

Department of Urology.

Purpose Of Review: Tissue engineering and regenerative medicine has emerged as a new scientific interdisciplinary field focusing on developing new strategies to repair or recreate tissues and organs. This review gathers findings on erectile dysfunction and, Peyronie's disease from recent preclinical and clinical studies under heading of stem-cell regenerative medicine.

Recent Findings: Over the last 2 years, preclinical studies on rat models demonstrated the tangible beneficial role of stem cells and stromal vascular fraction in the context of preventing fibrosis and restoring erectile function in different animal models of Erectile dysfunction and Peyronie's disease. Read More

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http://dx.doi.org/10.1097/MOU.0000000000000636DOI Listing

Integrin α9 depletion promotes β-catenin degradation to suppress triple negative breast cancer tumor growth and metastasis.

Int J Cancer 2019 Apr 22. Epub 2019 Apr 22.

Department of Toxicology and Cancer Biology, and Center for Research on Environment Disease, College of Medicine, University of Kentucky, Lexington, Kentucky.

Although integrin α9 (ITGA9) is known to be involved in cell adhesion and motility, its expression in cancer and its role in tumor growth and metastasis remain largely unknown. This study was designed to investigate the role of ITGA9 in triple negative breast cancer (TNBC). ITGA9 expression in TNBC cells were knocked out (KO) using CRISPR/Cas9 technology. Read More

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http://dx.doi.org/10.1002/ijc.32359DOI Listing

Variant Classification in Precision Oncology.

Int J Cancer 2019 Apr 22. Epub 2019 Apr 22.

Institute of Pathology, University Hospital Heidelberg, Germany.

Next-generation sequencing has become a cornerstone of therapy guidance in cancer precision medicine and an indispensable research tool in translational oncology. Its rapidly increasing use during the last decade has expanded the options for targeted tumor therapies, and molecular tumor boards have grown accordingly. However, with increasing detection of genetic alterations, their interpretation has become more complex and error-prone, potentially introducing biases and reducing benefits in clinical practice. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.32358
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http://dx.doi.org/10.1002/ijc.32358DOI Listing

[Advances in the roles and mechanisms of nonsense-mediated mRNA decay in embryonic development].

Sheng Li Xue Bao 2019 Apr;71(2):327-335

Institute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.

Nonsense-mediated mRNA decay (NMD) is originally identified as a widespread mRNA surveillance machinery in degrading 'aberrant' mRNA species with premature termination codons (PTCs) rapidly, which protects the cells from the accumulation of truncated proteins. Recent studies show that NMD can also regulate the degradation of normal gene transcripts, which execute important cellular and physiological functions. Therefore, NMD is considered as a highly conserved post-transcriptional regulatory mechanism in eukaryotes. Read More

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[The role of stem cell-derived exosomes in repairing myocardial injury].

Sheng Li Xue Bao 2019 Apr;71(2):205-215

School of Pharmaceutical Sciences of Guangzhou Medical University, Guangdong Key Laboratory of Molecular Target & Clinical Pharmacology, State Key Laboratory of Respiratory Diseases Pharmacology Group, Guangzhou 511436, China.

At present, it is generally believed that the paracrine effect of stem cells in the repair of myocardial injury is one of the important ways for stem cell therapy. Exosomes are phospholipid bilayer-enclosed nanovesicles that secreted by cells under physiological and pathological conditions. Cargo loaded into exosomes including protein, lipids and nucleic acids can be delivered to recipient cells. Read More

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April 2019
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Allogenic chondrocyte/osteoblast-loaded β-tricalcium phosphate bioceramic scaffolds for articular cartilage defect treatment.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):1570-1576

c Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product Processing , Ministry of Agriculture and Rural Affairs of the People's Republic of China , Shanghai , People's Republic of China.

The medical community has expressed significant interest in the treatment of cartilage defect. Successful repair of articular cartilage defects remains a challenge in clinics. Due to the huge advantages of 3D micro/nanomaterials, 3D artificial micro/nano scaffolds have been widely developed and explored in the tissue repair of articular joints. Read More

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http://dx.doi.org/10.1080/21691401.2019.1604534DOI Listing
December 2019

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):1533-1542

c Centre national de la recherche scientifique, LAGEP-UMR 5007, University Claude Bernard Lyon-1 , University of Lyon , Lyon , France.

Poly (methyl methacrylate) (PMMA) is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and the most important characteristics of PMMA is that it does not produce any toxicity. There is not much information about PMMA action on the colon cancer cells. In the present study, we have synthesized PMMA nanoparticles. Read More

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https://www.tandfonline.com/doi/full/10.1080/21691401.2019.1
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http://dx.doi.org/10.1080/21691401.2019.1577886DOI Listing
December 2019
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Transplantation of mouse iPSCs into testis of azoospermic mouse model: in vivo and in vitro study.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):1585-1594

c Department of Hematology and Stem Cell Therapy, Faculty of Medical Science , Tarbiat Modares University , Tehran , Iran.

This study aimed to induce spermatogenesis in azoospermic testis through induced pluripotent stem cells (iPSCs) derived spermatogonial stem cell-like cells (SSCLCs) after iPSCs in vivo and in vitro transplantation and three-dimensional organ culture. DiI-labelled mouse iPSCs were transplanted to azoospermic testis mouse model (pretreated by busulfan 40 mg/kg). This study was designed based on two experimental groups. Read More

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https://www.tandfonline.com/doi/full/10.1080/21691401.2019.1
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http://dx.doi.org/10.1080/21691401.2019.1594854DOI Listing
December 2019

MicroRNAs as regulators and effectors of hematopoietic transcription factors.

Wiley Interdiscip Rev RNA 2019 Apr 21:e1537. Epub 2019 Apr 21.

Department of Physiology, Center for Stem Cell Biology and Regenerative Medicine, Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland.

Hematopoiesis is a highly-regulated development process orchestrated by lineage-specific transcription factors that direct the generation of all mature blood cells types, including red blood cells, megakaryocytes, granulocytes, monocytes, and lymphocytes. Under homeostatic conditions, the hematopoietic system of the typical adult generates over 10 blood cells daily throughout life. In addition, hematopoiesis must be responsive to acute challenges due to blood loss or infection. Read More

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http://dx.doi.org/10.1002/wrna.1537DOI Listing

Educational attainment of childhood cancer survivors: A systematic review.

Cancer Med 2019 Apr 21. Epub 2019 Apr 21.

Institute of Health & Society, Newcastle University, Newcastle upon Tyne, United Kingdom.

Background: Advances in treatment mean that most children diagnosed with cancer during childhood survive. Therefore, it is increasingly important to examine the long-term consequences of childhood cancer, including educational attainment. This systematic review investigated whether the educational attainment of childhood cancer survivors differ from the cancer-free population. Read More

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http://dx.doi.org/10.1002/cam4.2154DOI Listing

A comparison of risk factors for metastasis at diagnosis in humans and dogs with osteosarcoma.

Cancer Med 2019 Apr 21. Epub 2019 Apr 21.

Division of Pediatric Epidemiology and Clinical Research, School of Medicine, University of Minnesota, Minneapolis, Minnesota.

Background: Canine osteosarcoma (OS) is a relevant spontaneous model for human OS. Identifying similarities in clinical characteristics associated with metastasis at diagnosis in both species may substantiate research aimed at using canine OS as a model for identifying mechanisms driving distant spread in the human disease.

Methods: This retrospective study included dog OS cases from three academic veterinary hospitals and human OS cases from the Surveillance, Epidemiology, and End Results program. Read More

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http://dx.doi.org/10.1002/cam4.2177DOI Listing

Cytokine-induced hematopoietic stem and progenitor cell mobilization: unraveling interactions between stem cells and their niche.

Ann N Y Acad Sci 2019 Apr 21. Epub 2019 Apr 21.

Section of Stem Cell Biology, Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands.

Peripheral blood hematopoietic stem and progenitor cells (HSPCs), mobilized by granulocyte colony-stimulating factor, are widely used as a source for both autologous and allogeneic stem cell transplantation. The use of mobilized HSPCs has several advantages over traditional bone marrow-derived HSPCs, including a less invasive harvesting process for the donor, higher HSPC yields, and faster hematopoietic reconstitution in the recipient. For years, the mechanisms by which cytokines and other agents mobilize HSPCs from the bone marrow were not fully understood. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/nyas.14059
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http://dx.doi.org/10.1111/nyas.14059DOI Listing
April 2019
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A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology.

DNA Res 2019 Apr 22. Epub 2019 Apr 22.

Department of Biomedical Sciences, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.

Urodele newts have unique biological properties, notably including prominent regeneration ability. The Iberian ribbed newt, Pleurodeles waltl, is a promising model amphibian distinguished by ease of breeding and efficient transgenic and genome editing methods. However, limited genetic information is available for P. Read More

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http://dx.doi.org/10.1093/dnares/dsz003DOI Listing

Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells.

Clin Hemorheol Microcirc 2019 Apr 19. Epub 2019 Apr 19.

Translational Center Regenerative Therapies (TLZ-RT), Wuerzburg branch of the Fraunhofer Institute for Silicate Research (ISC), Wuerzburg, Germany.

Background: Prolyl hydroxylase inhibitors (PHIs) are promising compounds to promote angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α), a master regulator of angiogenesis. Increased HIF-1α presence induces expression of proangiogenic genes such as vascular endothelial growth factor (VEGF).

Objective: We investigated the pharmacological induction of hypoxia via the PHI ciclopirox olamine (CPX) as angiogenesis strategy on human dermal microvascular endothelial cell (hd-mvEC) spheroids directly and indirectly via activating human mesenchymal stem cells (hMSCs). Read More

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http://dx.doi.org/10.3233/CH-190559DOI Listing

Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion.

Dev Cell 2019 Apr 9. Epub 2019 Apr 9.

Department of Molecular Pathophysiology, Institute of Advanced Medical Sciences, Nippon Medical School, 1-396 Kosugi-machi, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan. Electronic address:

Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium (HE) within the ventral portion of the dorsal aorta during vertebrate development. In zebrafish, Notch signaling induces HE specification from posterior lateral plate mesoderm (PLPM) cells as they migrate over the ventral surface of the somite. During migration, PLPM cells make close contact with Notch-ligand-expressing somitic cells to acquire HE identity. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.023DOI Listing

An SH3PX1-Dependent Endocytosis-Autophagy Network Restrains Intestinal Stem Cell Proliferation by Counteracting EGFR-ERK Signaling.

Dev Cell 2019 Apr 12. Epub 2019 Apr 12.

Huntsman Cancer Institute and Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112, USA. Electronic address:

The effect of intracellular vesicle trafficking on stem-cell behavior is largely unexplored. We screened the Drosophila sorting nexins (SNXs) and discovered that one, SH3PX1, profoundly affects gut homeostasis and lifespan. SH3PX1 restrains intestinal stem cell (ISC) division through an endocytosis-autophagy network that includes Dynamin, Rab5, Rab7, Atg1, 5, 6, 7, 8a, 9, 12, 16, and Syx17. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.03.029DOI Listing

Pathological ASXL1 Mutations and Protein Variants Impair Neural Crest Development.

Stem Cell Reports 2019 Apr 4. Epub 2019 Apr 4.

Institute for Stem Cell Research, Helmholtz Zentrum München GmbH, 85764 Neuherberg, Germany. Electronic address:

The neural crest (NC) gives rise to a multitude of fetal tissues, and its misregulation is implicated in congenital malformations. Here, we investigated molecular mechanisms pertaining to NC-related symptoms in Bohring-Opitz syndrome (BOS), a developmental disorder linked to mutations in the Polycomb group factor Additional sex combs-like 1 (ASXL1). Genetically edited human pluripotent stem cell lines that were differentiated to NC progenitors and then xenotransplanted into chicken embryos demonstrated an impairment of NC delamination and emigration. Read More

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http://dx.doi.org/10.1016/j.stemcr.2019.03.006DOI Listing

PAX3 Confers Functional Heterogeneity in Skeletal Muscle Stem Cell Responses to Environmental Stress.

Cell Stem Cell 2019 Apr 12. Epub 2019 Apr 12.

Inserm, IMRB U955-E10, 94000 Créteil, France; Faculté de Médecine, Université Paris Est Créteil, 94000 Créteil, France; Ecole Nationale Vétérinaire d'Alfort, 94700 Maisons-Alfort, France; Etablissement Francais du Sang, 94017 Créteil, France; APHP, Hopital Henri Mondor, DHU Pepsy & Centre de Référence des Maladies Neuromusculaires GNMH, 94000 Créteil, France. Electronic address:

Muscle satellite cells (MuSCs) are the quiescent muscle stem cells required for adult skeletal muscle repair. The impact of environmental stress such as pollution on MuSC behavior remains unexplored. We evaluated the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure, a ubiquitous and highly toxic pollutant, on MuSCs by combining in vivo mouse molecular genetic models with ex vivo studies. Read More

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http://dx.doi.org/10.1016/j.stem.2019.03.019DOI Listing

Lineage Tracing Reveals a Subset of Reserve Muscle Stem Cells Capable of Clonal Expansion under Stress.

Cell Stem Cell 2019 Apr 12. Epub 2019 Apr 12.

The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Orthopedic Surgery, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address:

Stem cell heterogeneity is recognized as functionally relevant for tissue homeostasis and repair. The identity, context dependence, and regulation of skeletal muscle satellite cell (SC) subsets remains poorly understood. We identify a minor subset of Pax7 SCs that is indelibly marked by an inducible Mx1-Cre transgene in vivo, is enriched for Pax3 expression, and has reduced ROS (reactive oxygen species) levels. Read More

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http://dx.doi.org/10.1016/j.stem.2019.03.020DOI Listing

Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs.

Cell Stem Cell 2019 Mar 27. Epub 2019 Mar 27.

School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address:

Expansion of transposable elements (TEs) coincides with evolutionary shifts in gene expression. TEs frequently harbor binding sites for transcriptional regulators, thus enabling coordinated genome-wide activation of species- and context-specific gene expression programs, but such regulation must be balanced against their genotoxic potential. Here, we show that Krüppel-associated box (KRAB)-containing zinc finger proteins (KZFPs) control the timely and pleiotropic activation of TE-derived transcriptional cis regulators during early embryogenesis. Read More

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http://dx.doi.org/10.1016/j.stem.2019.03.012DOI Listing

The molecular basis and genotype-phenotype correlations of congenital adrenal hyperplasia (CAH) in Anatolian population.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Department of Medical Genetics, Erciyes University, Kayseri, 38039, Turkey.

Congenital adrenal hyperplasia (CAH) is an autosomal recessive genetic disorder due to presence of mutations in the genes involved in the metabolism of steroid hormones in adrenal gland. There are two main forms of CAH, classic form and non-classic form. While classic form stands for the severe form, the non-classic form stands for the moderate and more frequent form of CAH. Read More

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http://link.springer.com/10.1007/s11033-019-04809-4
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http://dx.doi.org/10.1007/s11033-019-04809-4DOI Listing
April 2019
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Spinal Cord Injury: Animal Models, Imaging Tools and the Treatment Strategies.

Neurochem Res 2019 Apr 20. Epub 2019 Apr 20.

Inserm, U-1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, Université de Lille, 59000, Lille, France.

Spinal cord injury (SCI) often leads to irreversible neuro-degenerative changes with life-long consequences. While there is still no effective therapy available, the results of past research have led to improved quality of life for patients suffering from partial or permanent paralysis. In this review we focus on the need, importance and the scientific value of experimental animal models simulating SCI in humans. Read More

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http://link.springer.com/10.1007/s11064-019-02800-w
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http://dx.doi.org/10.1007/s11064-019-02800-wDOI Listing
April 2019
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Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways.

Phytomedicine 2018 Nov 14;59:152755. Epub 2018 Nov 14.

Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. Electronic address:

Background: Kaempferia parviflora Wall. ex Baker (KP) has long been used in traditional medicine to treat various diseases because active compounds in rhizome extracts are important anti-inflammatory agents.

Purpose: This study aims to investigate the effects of an ethanolic extract of KP on the molecular mechanisms associated with rheumatoid arthritis (RA), which was induced by a combination of proinflammatory cytokines (IL-1β or TNF-α with IL-17A) in a human synovial sarcoma cell line (SW982) culture model. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.11.015DOI Listing
November 2018

Green propolis extract promotes in vitro proliferation, differentiation, and migration of bone marrow stromal cells.

Biomed Pharmacother 2019 Apr 18;115:108861. Epub 2019 Apr 18.

Tissue Regeneration Laboratory, Department of Large Animal Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA. Electronic address:

Background: Propolis is a resinous material extracted from bee glue with a complex chemical composition. The unique biological properties of propolis have led to its use in alternative medicine and as a nutritional supplement. Recent research shows that propolis could affect the immune system by decreasing the production of inflammatory cytokines and potentiating an effect on resident stem cells. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108861DOI Listing

Cyclic tensile stress promotes osteogenic differentiation of adipose stem cells via ERK and p38 pathways.

Stem Cell Res 2019 Apr 8;37:101433. Epub 2019 Apr 8.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address:

The present study aimed to elucidate whether extracellular signal-regulated kinases 1/2 (ERK1/2) and p38 mitogen-activated protein kinases pathways participate in the transduction of mechanical stretch exerted on adipose stem cells (ASCs) into intracellular osteogenic signals, and if so whether both pathways have time-dependent feature. Rat ASCs were cultured in osteogenic medium for 72 h and assigned into three sets, namely ERK1/2 inhibitor treated set, p38 inhibitor treated set, and the control set. After inhibitor treatment, all cells were subjected to cyclic stretch(2000 με, 1 Hz) on a four-point bending mechanical loading device. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18735061193006
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http://dx.doi.org/10.1016/j.scr.2019.101433DOI Listing
April 2019
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Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.

Stem Cell Res 2019 Apr 15;37:101439. Epub 2019 Apr 15.

Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Japan; Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Japan. Electronic address:

Cynomolgus monkey ES (Cyn ES) cells can be generated in a similar manner as human ES cells. However, Cyn ES cells are difficult to maintain in an undifferentiated state by untrained researchers. For easier culture, we generated an OCT3/4-P2A tdTomato IRES Zeocin Cyn ES cell line using CRISPR/Cas9 genome editing technology. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101439DOI Listing

Anti-metastasis activity of curcumin against breast cancer via the inhibition of stem cell-like properties and EMT.

Phytomedicine 2018 Nov 12;58:152740. Epub 2018 Nov 12.

The Research Center of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address:

Background: Curcumin is a polyphenolic compound with potent chemopreventive and anti-cancer efficacy.

Purpose: To explore the potential anti-metastasis efficacy of curcumin in breast cancer stem-like cells (BCSCs), which are increasingly considered to be the origin of the recurrence and metastasis of breast cancer.

Methods: A CCK8 assay was performed to evaluate cell viability, and a colony formation assay was conducted to determine cell proliferation in MCF-7 and MDA-MB-231 adherent cells. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.11.001DOI Listing
November 2018

Engraftment of Human Stem Cell-Derived Otic Progenitors in the Damaged Cochlea.

Mol Ther 2019 Apr 2. Epub 2019 Apr 2.

CNRS UMR 7260, Aix-Marseille Université, Marseille, France; Laboratory of Bioengineering and Nanoscience, LBN, University of Montpellier, Montpellier, France. Electronic address:

Most cases of sensorineural deafness are caused by degeneration of hair cells. Although stem/progenitor cell therapy is becoming a promising treatment strategy in a variety of organ systems, cell engraftment in the adult mammalian cochlea has not yet been demonstrated. In this study, we generated human otic progenitor cells (hOPCs) from induced pluripotent stem cells (iPSCs) in vitro and identified these cells by the expression of known otic markers. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15250016193011
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http://dx.doi.org/10.1016/j.ymthe.2019.03.018DOI Listing
April 2019
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Survivability of rabbit amniotic fluid-derived mesenchymal stem cells post slow-freezing or vitrification.

Acta Histochem 2019 Apr 17. Epub 2019 Apr 17.

Research Institute for Animal Production in Nitra, NAFC, Hlohovecká 2, 951 41 Lužianky, Slovak Republic; Faculty of Biotechnology and Food Science, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic; Faculty of Animal Breeding and Biology, University of Technology and Life Sciences, Al. prof. S. Kaliskiego 7, 85 796 Bydgoszcz, Poland.

This work aimed to evaluate the effect of two distinct cryopreservation procedures - conventional slow-freezing and vitrification, on survivability and mesenchymal marker expression stability of rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs). Cells at passage 2 were slowly frozen, using 10% of dimethylsulfoxide, or vitrified, using 40% of ethylene glycol, 0.5 M sucrose and 18% Ficoll 70. Read More

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http://dx.doi.org/10.1016/j.acthis.2019.03.008DOI Listing

So You Want to Give Stem Cells to Babies? Neonatologists and Parents' Views to Optimize Clinical Trials.

J Pediatr 2019 Apr 17. Epub 2019 Apr 17.

Clinical Epidemiology Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada; School of Epidemiology and Public Health, University of Ottawa, Ottawa, Ontario, Canada.

Objective: To identify barriers and enablers that may influence parents' and neonatologists' participation in clinical trials of mesenchymal stromal cells for bronchopulmonary dysplasia.

Study Design: This qualitative study involved one-on-one semistructured interviews with parents of extremely preterm infants (n = 18) and neonatologists (n = 16). Interview guides and directed content analysis were framed using the theoretical domains framework, a tool specifically developed for implementation research to identify influences on behavior. Read More

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http://dx.doi.org/10.1016/j.jpeds.2019.03.005DOI Listing
April 2019
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Generation of induced pluripotent stem cell line-NTUHi001-A from a premature ovarian failure patient with Turner's syndrome mosaicism.

Stem Cell Res 2019 Apr 2;37:101422. Epub 2019 Apr 2.

Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei, Taiwan. Electronic address:

Turner's syndrome (TS) is one of the main causes of premature ovarian failure (POF). However, the mechanisms underlying POF are difficult to study due to the lack of suitable disease models. Herein, we have generated a human induced pluripotent stem cell (hiPSC) line derived from the peripheral blood mononuclear cells of a female patient with Turner's syndrome mosaicism via integration-free Sendai-virus system. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101422DOI Listing
April 2019
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Generation of induced pluripotent stem cells (iPSCs) IRMBi002-A from an Alzheimer's disease patient carrying a D694N mutation in the APP gene.

Stem Cell Res 2019 Apr 15;37:101438. Epub 2019 Apr 15.

Institute for Regenerative Medicine and Biotherapy (IRMB), Montpellier, France; Hopital St Eloi, CHU Montpellier, 80 rue augustin Fliche, 30295 Montpellier, France. Electronic address:

Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 58 year-old woman suffering from Alzheimer's disease and carrying a D694N mutation on Amyloid precursor protein (APP). Fibroblasts were reprogrammed into iPSC using the integration-free Sendai Virus which allows the expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101438DOI Listing

A Microparticle Approach for Non-viral Gene Delivery within 3D Human Mesenchymal Stromal Cell Aggregates.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin, Madison, WI 53706, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA; AO Foundation Collaborative Research Center, Davos, Switzerland. Electronic address:

Three-dimensional (3D) multicellular aggregates, in comparison to two-dimensional monolayer culture, can provide tissue culture models that better recapitulate the abundant cell-cell and cell-matrix interactions found in vivo. In addition, aggregates are potentially useful building blocks for tissue engineering. However, control over the interior aggregate microenvironment is challenging due to inherent barriers for diffusion of biological mediators (e. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.04.038DOI Listing

Primary cilium and brain aging: role in neural stem cells, neurodegenerative diseases and glioblastoma.

Ageing Res Rev 2019 Apr 17. Epub 2019 Apr 17.

Cellular Oncology group, Biodonostia Health Research Institute, San Sebastian, Spain; CIBER de Fragilidad y Envejecimiento Saludable (CIBERfes), Madrid, Spain; IKERBASQUE, Basque Foundation, Bilbao, Spain. Electronic address:

Brain aging is characterized by a progressive loss of tissue integrity and function as a consequence of impaired homeostasis and regeneration capacities. The primary cilium is a highly conserved organelle that projects from the cell surface in a single copy in virtually all mammalian cell types including neural stem/progenitors cells and neurons. Increasing evidence in the last decade points out that primary cilium could be a relevant mediator of neural stem cell activity, neurogenesis, neuronal maturation and maintenance, and brain tumorigenesis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15681637183030
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http://dx.doi.org/10.1016/j.arr.2019.04.004DOI Listing
April 2019
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Differential Effects of Unipolar versus Bipolar Depression on Episodic Memory Updating.

Neurobiol Learn Mem 2019 Apr 17. Epub 2019 Apr 17.

National Center for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India; Centre for Brain Development and Repair, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India; Centre for Discovery Brain Sciences, Deanery of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, 15 George Square, Edinburgh EH89XD, UK.

Episodic memories, when reactivated, can be modified or updated by new learning. Since such dynamic memory processes remain largely unexplored in psychiatric disorders, we examined the impact of depression on episodic memory updating. Unipolar and bipolar depression patients, and age/education matched controls, first learned a set of objects (List-1). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10747427193007
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http://dx.doi.org/10.1016/j.nlm.2019.04.008DOI Listing
April 2019
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Evaluating laboratory criteria for combined immunodeficiency in adult patients diagnosed with common variable immunodeficiency.

Clin Immunol 2019 Apr 17. Epub 2019 Apr 17.

Department for Rheumatology and Clinical Immunology and Center for Chronic Immunodeficiency (CCI), Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany; Center for Chronic Immunodeficiency (CCI), Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany. Electronic address:

Some patients diagnosed with common variable immunodeficiency (CVID) actually suffer from combined immunodeficiency (CID) and therefore may require a different, CID-adapted treatment. Several CD4 T-cell-based criteria have been proposed in the past to identify patients with CID within the cohort of adult CVID patients. In this monocentric study, we used retrospective immunological and clinical data of 238 CVID patients to compare four different proposals of how to define CID among CVID patients. Read More

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http://dx.doi.org/10.1016/j.clim.2019.04.001DOI Listing

The patient-independent human iPSC model - a new tool for rapid determination of genetic variant pathogenicity in long QT syndrome.

Heart Rhythm 2019 Apr 17. Epub 2019 Apr 17.

(1)Vanderbilt University School of Medicine, Nashville, TN, USA; (2)Vanderbilt Center for Arrhythmia Research and Therapeutics, Department of Medicine, Nashville, TN, USA. Electronic address:

Background: Commercial genetic testing for Long QT Syndrome (LQTS) has rapidly expanded, but the inability to accurately predict whether a rare variant is pathogenic has limited its clinical benefit. Novel missense variants are routinely reported as "Variant of Unknown Significance (VUS)" and cannot be used to screen family members at-risk for sudden cardiac death. Better approaches to determine pathogenicity of VUS are needed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15475271193036
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http://dx.doi.org/10.1016/j.hrthm.2019.04.031DOI Listing
April 2019
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Human Erythroblasts with c-Kit Activating Mutations Have Reduced Cell Culture Costs and Remain Capable of Terminal Maturation.

Exp Hematol 2019 Apr 17. Epub 2019 Apr 17.

Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester, Rochester, NY. Electronic address:

A major barrier to the in vitro production of red blood cells for transfusion therapy is the cost of culture components, with cytokines making up greater than half of the culture costs. Cell culture cytokines also represent a major expense for in vitro studies of human erythropoiesis. HUDEP2 cells are an E6/E7 immortalized erythroblast line used for the in vitro study of human erythropoiesis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0301472X193013
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http://dx.doi.org/10.1016/j.exphem.2019.04.001DOI Listing
April 2019
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Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Ann Neurol 2019 Apr 20. Epub 2019 Apr 20.

Biomedical Science and Engineering Interdisciplinary Program, KAIST, Daejeon, Korea.

Objective: JBTS17 is a major gene mutated in ciliopathies such as Joubert syndrome and oral-facial-digital syndrome type VI. Most patients with loss of function mutations in JBTS17 exhibit cerebellar vermis hypoplasia and brain stem malformation. However, some patients with JBTS17 mutations show microcephaly and abnormal gyration. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/ana.25491
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http://dx.doi.org/10.1002/ana.25491DOI Listing
April 2019
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Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments.

Stem Cells Transl Med 2019 Apr 19. Epub 2019 Apr 19.

University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, Iowa, USA.

Subretinal delivery of stem cell-derived retinal cells as a strategy to treat retinal degenerative blindness holds great promise. Currently, two clinical trials are underway in which human fetal retinal progenitor cells (RPCs) are being delivered to patients by intravitreal or subretinal injection to preserve or restore vision, respectively. With the advent of the induced pluripotent stem cell (iPSC), and in turn three-dimensional derivation of retinal tissue, it is now possible to generate autologous RPCs for cell replacement. Read More

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http://dx.doi.org/10.1002/sctm.18-0210DOI Listing

Lung clearance index for early detection of pulmonary complications after allo-HSCT in children.

Pediatr Pulmonol 2019 Apr 19. Epub 2019 Apr 19.

Department of Pediatrics and Adolescent Medicine, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Background: Pulmonary chronic graft-vs-host disease (cGvHD) after hematopoietic stem cell transplantation (HSCT) is characterized by impairment of the small airways. Assessment of lung clearance index (LCI) gained from multiple breath washout (MBW) is more sensitive than spirometry in detection of small airways disease. The aim of this study was to describe the development of LCI during the first year after pediatric HSCT and how LCI relates to other pulmonary function parameters and cGvHD. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/ppul.24340
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http://dx.doi.org/10.1002/ppul.24340DOI Listing
April 2019
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Low-dose aspirin reduces hypoxia-induced sFlt1 release via the JNK/AP-1 pathway in human trophoblast and endothelial cells.

J Cell Physiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China.

Pre-eclampsia (PE) is a serious hypertensive disorder of pregnancy that remains a leading cause of perinatal and maternal morbidity and mortality worldwide. Placental ischemia/hypoxia and the secretion of soluble fms-like tyrosine kinase 1 (sFlt1) into maternal circulation are involved in the pathogenesis of PE. Although low-dose aspirin (LDA) has beneficial effects on the prevention of PE, the exact mechanisms of action of LDA, particularly on placental dysfunction, and sFlt1 release, have not been well investigated. Read More

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http://dx.doi.org/10.1002/jcp.28533DOI Listing

Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model.

J Cell Physiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28680
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http://dx.doi.org/10.1002/jcp.28680DOI Listing
April 2019
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