2,688 results match your criteria Stem Cells and Development [Journal]


Menstrual blood-derived stem cells as delivery vehicles for oncolytic adenovirus virotherapy for colorectal cancer.

Stem Cells Dev 2019 Apr 16. Epub 2019 Apr 16.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China ;

Oncolytic adenoviruses (Ads) have potential applications in cancer therapy due to their ability to replicate and induce tumor cell death. However, their clinical application has been limited by the lack of efficient cell based delivery systems that can provide protection from immune attack and prevent virus clearance by neutralizing antibodies. We previously demonstrated that menstrual blood-derived mesenchymal stem cells (MenSCs) can specifically target tumor cells and serve as a novel drug delivery platform. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0222
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http://dx.doi.org/10.1089/scd.2018.0222DOI Listing
April 2019
1 Read

Human pluripotent stem cells as tools for predicting developmental neural toxicity of chemicals: strategies, applications and challenges.

Stem Cells Dev 2019 Apr 16. Epub 2019 Apr 16.

China ;

The human central nervous system (CNS) is very sensitive to perturbations, since it performs sophisticated biological processes and requires cooperation from multiple neural cell types. Subtle interference from exogenous chemicals, such as environmental pollutants, industrial chemicals, drug components, food additives and cosmetics' constituents, may initiate severe developmental neural toxicity (DNT). Human pluripotent stem cell (hPSC)-based neural differentiation assays provide effective and promising tools to help evaluate potential DNT caused by those toxicants. Read More

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http://dx.doi.org/10.1089/scd.2019.0007DOI Listing

Clinical features of chronic graft-versus-host disease following haploidentical transplantation combined with infusion of a cord blood.

Stem Cells Dev 2019 Apr 12. Epub 2019 Apr 12.

Department of Respiratory Medicine, the Fifth People's Hospital of Suzhou, Suzhou, China, China ;

Our previous studies demonstrated promising outcomes after haploidentical donor transplant combined with unrelated umbilical cord blood (haplo-cord-HSCT) for hematological disorders. However, clinical profiling regarding chronic graft-versus-host disease (cGVHD) has not yet been fully described under this protocol. This study analyzed the clinical characteristics of cGVHD among 300 patients with hematological malignancies who received haplo-cord-HSCT between January 2012 and July 2016 at our center. Read More

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http://dx.doi.org/10.1089/scd.2018.0259DOI Listing
April 2019
1 Read

Dental Follicle Stem Cells: Tissue Engineering and Immunomodulation.

Stem Cells Dev 2019 Apr 10. Epub 2019 Apr 10.

Department of Orthodontics, the Affiliated Hospital of Qingdao University; School of Stomatology, Qingdao University, Qingdao, Shandong, China ;

Dental follicle stem cells (DFSCs), which are the stem cells present in the dental follicle tissue of the tooth germ and are derived from the neural crest, are the direct precursor cells of periodontal tissues and can form the periodontal ligament, cementum and alveolar bone proper in the late stage of tooth development. DFSCs have the ability to achevie osteogenic, adipogenic, chondrogenic, neural, and cardiomyocytic differentiation in a specific induced environment. Recently, isolated dental follicle epithelial stem cells from DFSCs were also found to have the ability to form salivary gland cells and ductal cells. Read More

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http://dx.doi.org/10.1089/scd.2019.0012DOI Listing

miR-384-5p targets Gli2 and negatively regulates age-related osteogenic differentiation of rat bone marrow mesenchymal stem cells.

Stem Cells Dev 2019 Apr 5. Epub 2019 Apr 5.

Changhai Hospital, 12520, Department of Orthopedic, Shanghai, China , 200433 ;

Aberrant microRNA (miRNA) expression correlates with age-related osteoporosis, which impairs bone formation by regulating osteoblastic activity, thus leading to age-related bone loss. In this study, we observed that miR-384-5p was significantly upregulated in bone marrow mesenchymal stem cells (BMSCs) from aged rats as compared with BMSCs from young rats. In vitro functional assays revealed that overexpression of miR-384-5p in young BMSCs inhibited osteogenic differentiation and accelerated senescence, whereas a knockdown of miR-384-5p in aged BMSCs had the opposite effects. Read More

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http://dx.doi.org/10.1089/scd.2019.0044DOI Listing
April 2019
2 Reads

LncRNA H19 knockdown in human amniotic mesenchymal stem cells suppresses angiogenesis by associating with EZH2 and activating VASH1.

Stem Cells Dev 2019 Apr 2. Epub 2019 Apr 2.

Suzhou University, 12582, The Affiliated stomatological Hospital of Soochow University,Suzhou Stomatological Hospital, Suzhou, Jiangsu, China.

Human amniotic mesenchymal stem cells (HAMSCs) are promising seed cells with great advantages in promoting angiogenesis. However, the mechanisms underlying angiogenesis facilitated by HAMSCs are still unclear. Long noncoding RNA (lncRNA) H19 is involved in many biological processes, such as enhancing angiogenesis and proliferation, invasion, and migration of cancer cells. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2019.0014
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http://dx.doi.org/10.1089/scd.2019.0014DOI Listing
April 2019
6 Reads

Glycan profiling analysis of equine amniotic progenitor mesenchymal cells and their derived extracellular microvesicles.

Stem Cells Dev 2019 Mar 22. Epub 2019 Mar 22.

Università degli Studi di Milano, 9304, Dipartimento di Medicina Veterinaria, Milano, Lombardia, Italy ;

Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes. Their secreted vesicles are important effectors in a wide array of biological processes and contribute to in vivo healing of equine tendon lesions and endometrial inflammation. Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells. Read More

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http://dx.doi.org/10.1089/scd.2019.0013DOI Listing

Sublethal Doses of Zinc Protect Neural Stem Cells Against Hypoxia through Activation of the PI3K Pathway.

Stem Cells Dev 2019 Mar 21. Epub 2019 Mar 21.

Hanyang University, Neurology , 17 Haengdang-dong , Seonggong-gu , Seoul, Korea, Republic of , 133-791.

Cerebral infarction is one of the major causes of severe morbidity and mortality, and thus, research has focused on developing treatment options for this condition. Zinc (Zn) is an essential element in the central nervous system and has several neuroprotective effects in the brain. In the present study, we examined the neuroprotective effects of Zn on neural stem cells (NSCs) exposed to hypoxia. Read More

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http://dx.doi.org/10.1089/scd.2018.0138DOI Listing

Exosomes Derived from human umbilical cord Mesenchymal Stem Cells Promote Fibroblast-to-Myofibroblast Differentiation in Inflammatory Environments and Benefit Cardioprotective Effects.

Stem Cells Dev 2019 Mar 21. Epub 2019 Mar 21.

Jiangsu University, School of Medical Science and Laboratory Medicine, China ;

Cardioprotective effects of exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-exosomes) post-myocardial infarction (post-MI) have been reported in our previous study. It is known that fibroblasts are pro-inflammatory phenotypes while myofibroblasts are anti-inflammatory phenotypes. This study aimed to investigate whether hucMSC-exosomes promoted cardiac fibroblast-to-myofibroblast differentiation in inflammatory environments and protected cardiomyocytes. Read More

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http://dx.doi.org/10.1089/scd.2018.0242DOI Listing
March 2019
1 Read
3.727 Impact Factor

Single-Cell RNA-Sequencing and Optical Electrophysiology of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Reveal Discordance Between Cardiac Subtype-Associated Gene Expression Patterns and Electrophysiological Phenotypes.

Stem Cells Dev 2019 Apr 17. Epub 2019 Apr 17.

1 Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota.

The ability to accurately phenotype cells differentiated from human induced pluripotent stem cells (hiPSCs) is essential for their application in modeling developmental and disease processes, yet also poses a particular challenge without the context of anatomical location. Our specific objective was to determine if single-cell gene expression was sufficient to predict the electrophysiology of iPSC-derived cardiac lineages, to evaluate the concordance between molecular and functional surrogate markers. To this end, we used the genetically encoded voltage indicator ArcLight to profile hundreds of hiPSC-derived cardiomyocytes (hiPSC-CMs), thus identifying patterns of electrophysiological maturation and increased prevalence of cells with atrial-like action potentials (APs) between days 11 and 42 of differentiation. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2019.0030
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http://dx.doi.org/10.1089/scd.2019.0030DOI Listing
April 2019
12 Reads

Inhibition of Ezh2 in vitro and the decline of Ezh2 in developing midbrain promotes dopaminergic neurons differentiation through modifying H3K27me3.

Stem Cells Dev 2019 Mar 19. Epub 2019 Mar 19.

Shanghai Institute of Materia Medica Chinese Academy of Sciences, 58298, Department of Neuropharmacology , 555, Zu Chong Zhi Road, Shanghai, People's Republic of China , Shanghai, China , 201203.

Epigenetic modifications play an important role in neural development. Trimethylated histone H3 at lysine 27 (H3K27me3) is a repressive epigenetic mark that mediates tissue development. Here, we demonstrated that H3K27me3 and histone methyl-transferase Ezh2 regulated the development of dopaminergic neurons in vitro and in vivo. Read More

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http://dx.doi.org/10.1089/scd.2018.0258DOI Listing

In vitro conditioning determines the capacity of Dental Pulp Stem Cells to function as pericyte-like cells.

Stem Cells Dev 2019 Mar 19. Epub 2019 Mar 19.

Rieti University Hub "Sabina Universitas", Laboratory of Experimental Medicine and Environmental Pathology, Rieti, Italy.

Dental pulp has revealed as an accessible and rich source of mesenchymal stem cells (MSCs) and its biological potential is currently under intense investigation. MSCs from dental pulp (DPSCs) have been indicated as a heterogeneous population oriented not only in repairing dentine but also in maintaining vascular and nervous homeostasis of the teeth. We sought to verify the phenotype of cells isolated from dental pulp of young donors and to investigate in vitro their role as pericyte-like cells. Read More

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http://dx.doi.org/10.1089/scd.2018.0192DOI Listing

Generation of anterior hindbrain-specific, glial-restricted progenitor-like cells from human pluripotent stem cells.

Stem Cells Dev 2019 Mar 18. Epub 2019 Mar 18.

Korea University, The Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology , Korea University, 1, 5-ka, Anam-dong, Sungbuk-Gu, Seoul 136-701 , Seoul, Korea, Republic of , 136-701.

Engraftment of oligodendrocyte progenitor cells (OPCs), which form myelinating oligodendrocytes, has the potential to treat demyelinating diseases such as multiple sclerosis. However, conventional strategies for generating oligodendrocytes have mainly focused on direct differentiation into forebrain- or spinal cord-restricted oligodendrocytes without establishing or amplifying stem/progenitor cells. Taking advantage of a recently established culture system, we generated expandable EN1- and GBX2-positive glial-restricted progenitor-like cells (GPLCs) near the anterior hindbrain. Read More

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http://dx.doi.org/10.1089/scd.2019.0033DOI Listing
March 2019
1 Read

WNT Inhibition and Increased Fibroblast Growth Factor Signaling Promotes Derivation of Less Heterogeneous Primed Human Embryonic Stem Cells, Compatible with Differentiation.

Stem Cells Dev 2019 Apr 2. Epub 2019 Apr 2.

1 Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.

Human embryonic stem cells (hESCs) hold great value for future clinical applications. However, standard culture conditions maintain hESCs in a primed state, which bears heterogeneity in pluripotency and a tendency for spontaneous differentiation. To counter these drawbacks, primed hESCs have been converted to a naive state, but this has restricted the efficiency of existing directed differentiation protocols. Read More

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http://dx.doi.org/10.1089/scd.2018.0199DOI Listing

Dentinogenesis and Tooth-Alveolar Bone Complex Defects in BMP9/GDF2 Knockout Mice.

Stem Cells Dev 2019 Apr 2. Epub 2019 Apr 2.

1 Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.

Tooth development is regulated by sequential and reciprocal epithelium-mesenchymal interactions and their related molecular signaling pathways, such as bone morphogenetic proteins (BMPs). Among the 14 types of BMPs, BMP9 (also known as growth differentiation factor 2) is one of the most potent BMPs to induce osteogenic differentiation of mesenchymal stem cells. The purpose of this study was to examine potential roles of BMP9 signaling in tooth development. Read More

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http://dx.doi.org/10.1089/scd.2018.0230DOI Listing
April 2019
2 Reads

Lipopolysaccharides Improve Mesenchymal Stem Cell-Mediated Cardioprotection by MyD88 and stat3 Signaling in a Mouse Model of Cardiac Ischemia/Reperfusion Injury.

Stem Cells Dev 2019 Apr 11. Epub 2019 Apr 11.

1 Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana.

Bone marrow-derived mesenchymal stem cells (MSCs) improve cardiac function after ischemia/reperfusion injury, in part, due to the release of cytoprotective paracrine factors. Toll-like receptor 4 (TLR4) is expressed in MSCs and regulates the expression of cytoprotective factors, cytokines, and chemokines. Lipopolysaccharide (LPS) stimulation of TLR4 activates two distinct signaling pathways that are either MyD88 dependent or MyD88 independent/TIR-domain-containing adapter-inducing interferon-β (TRIF) dependent. Read More

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http://dx.doi.org/10.1089/scd.2018.0213DOI Listing
April 2019
2 Reads

Human Bone Marrow Mesenchymal Stem Cells Rescue Endothelial Cells Experiencing Chemotherapy Stress by Mitochondrial Transfer via Tunnelling Nanotubes.

Stem Cells Dev 2019 Feb 27. Epub 2019 Feb 27.

Beijing Key Laboratory of Haematopoietic Stem Cell Transplantation, Institute of Haematology, Peking University People's Hospital, Beijing, China ;

Tunnelling nanotubes (TNTs) are newly discovered tubular structures between two distant cells that facilitate the intercellular exchange of signals and components. Recent reports show that mesenchymal stem cells (MSCs) can rescue injured target cells and promote recovery from a variety of stresses via TNT-mediated mitochondrial transfer. In this study, we explored how TNTs form between bone marrow MSCs and endothelial cells by using a human umbilical cord vein endothelial cell (HUVEC) cell model. Read More

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http://dx.doi.org/10.1089/scd.2018.0248DOI Listing
February 2019
1 Read

Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential.

Stem Cells Dev 2019 Apr 3. Epub 2019 Apr 3.

2 Institute of Genetic Medicine, Cardiovascular Research Centre, International Centre for Life, Newcastle University, Newcastle upon Tyne, United Kingdom.

Cardiac stem/progenitors are being used in the clinic to treat patients with a range of cardiac pathologies. However, improvements in heart function following treatment have been reported to be variable, with some showing no response. This discrepancy in response remains unresolved. Read More

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http://dx.doi.org/10.1089/scd.2018.0170DOI Listing
April 2019
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Induction of Mouse Peritoneum Mesenchymal Stem Cells into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media.

Stem Cells Dev 2019 Apr 30;28(8):554-564. Epub 2019 Mar 30.

2 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell-like cells using human follicular fluid (HFF) and human cumulus-conditioned medium (HCCM). Anterior abdominal wall and intestinal peritoneum explants were used for cells isolation and cultured in Dulbecco's modified Eagle's medium. Read More

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http://dx.doi.org/10.1089/scd.2018.0149DOI Listing
April 2019
3 Reads

Isolation and Characterization of Cerebellum-Derived Stem Cells in Poststroke Human Brain.

Stem Cells Dev 2019 Apr 18;28(8):528-542. Epub 2019 Mar 18.

1 Department of Neurosurgery, Hyogo College of Medicine, Nishinomiya, Japan.

There is compelling evidence that the mature central nervous system (CNS) harbors stem cell populations outside conventional neurogenic regions. We previously demonstrated that brain pericytes (PCs) in both mouse and human exhibit multipotency to differentiate into various neural lineages following cerebral ischemia. PCs are found throughout the CNS, including cerebellum, but it remains unclear whether cerebellar PCs also form ischemia-induced multipotent stem cells (iSCs). Read More

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http://dx.doi.org/10.1089/scd.2018.0232DOI Listing

Biodistribution of Glial Progenitors in a Three Dimensional-Printed Model of the Piglet Cerebral Ventricular System.

Stem Cells Dev 2019 Apr 28;28(8):515-527. Epub 2019 Mar 28.

1 Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

White matter damage persists in hypoxic-ischemic newborns even when treated with hypothermia. We have previously shown that intraventricular delivery of human glial progenitors (GPs) at the neonatal stage is capable of replacing abnormal host glia and rescuing the lifespan of dysmyelinated mice. However, such transplantation in the human brain poses significant challenges as related to high-volume ventricles and long cell migration distances. Read More

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http://dx.doi.org/10.1089/scd.2018.0172DOI Listing
April 2019
1 Read

Proteomic Analysis Reveals Temporal Changes in Protein Expression in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes In Vitro.

Stem Cells Dev 2019 Mar 20. Epub 2019 Mar 20.

1 Myocardial Function, National Heart and Lung Institute, Imperial College, London, United Kingdom.

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) hold great promise for regenerative medicine and in vitro screening. Despite displaying key cardiomyocyte phenotypic characteristics, they more closely resemble fetal/neonatal cardiomyocytes, and further characterization is necessary. By combining the use of tandem mass tags to label cell lysates, followed by multiplexing, we have determined the effects of short-term (30 day) in vitro culture on hiPSC-CM protein expression. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0210
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http://dx.doi.org/10.1089/scd.2018.0210DOI Listing
March 2019
10 Reads

Comparison of Cultured Human Cardiomyocyte Clusters Obtained from Embryos/Fetuses or Derived from Human Embryonic Stem Cells.

Stem Cells Dev 2019 Mar 26. Epub 2019 Mar 26.

2 Laboratory of Reproductive Biology, University Hospital of Copenhagen, Copenhagen, Denmark.

Cardiomyocytes (CMs) derived from human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) are used to study cardiogenesis and mechanisms of heart disease, and are being used in methods for toxiological screening of drugs. The phenotype of stem-cell-derived CMs should ideally resemble native CMs. Here, we compare embryonic/fetal CMs with hESC-derived CMs according to function and morphology. Read More

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http://dx.doi.org/10.1089/scd.2018.0231DOI Listing
March 2019
1 Read

Human Bone Marrow Mesenchymal Stem Cells Functionalized by Hybrid Baculovirus-Adeno-Associated Viral Vectors for Targeting Hypopharyngeal Carcinoma.

Stem Cells Dev 2019 Apr 11;28(8):543-553. Epub 2019 Mar 11.

1 Department of Otolaryngology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Hypopharyngeal carcinoma is a common malignant tumor of the head and neck with a very poor prognosis; the median survival time for curatively treated patients was 17.2 months in India. However, cell-based gene therapy holds promise to improve patient outcomes. Read More

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http://dx.doi.org/10.1089/scd.2018.0252DOI Listing
April 2019
3 Reads

Meta-Analysis of the Safety and Efficacy of Stem Cell Therapies for Ischemic Stroke in Preclinical and Clinical Studies.

Stem Cells Dev 2019 Apr;28(8):497-514

Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Haizhu District, Guangzhou, China.

Recent studies have indicated that stem cell transplantation may be effective in the treatment of ischemic stroke. Therefore, we performed a meta-analysis to evaluate the safety and efficacy of stem cell therapy for ischemic stroke in preclinical and clinical studies. In accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we searched the PubMed, Cochrane Library, Embase, Web of science, and Ovid databases from inception through May 2018. Read More

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http://dx.doi.org/10.1089/scd.2018.0218DOI Listing
April 2019
5 Reads
3.727 Impact Factor

Effects of Gastric Cancer Cell-Derived Exosomes on the Immune Regulation of Mesenchymal Stem Cells by the NF-kB Signaling Pathway.

Stem Cells Dev 2019 Apr 5;28(7):464-476. Epub 2019 Mar 5.

1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing Key Laboratory (No. BZO381), Beijing, People's Republic of China.

Mesenchymal stem cells (MSCs) are important components of the tumor microenvironment, which play an important role in tumor development. Exosomes derived from tumor cells can affect the biological characteristics of MSCs. Our study examined the effects of exosomes derived from gastric cancer cells on MSC immunomodulatory functions. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0125
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http://dx.doi.org/10.1089/scd.2018.0125DOI Listing
April 2019
15 Reads

Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.

Stem Cells Dev 2019 Apr 5;28(7):454-463. Epub 2019 Mar 5.

1 Medical College, Qingdao University, Qingdao, China.

Eye is a complex organ with a highly specialized tissue structure. The establishment of human pluripotent stem cells (hPSCs) has allowed the simulation of eye development in vitro. Most differentiation works of hPSC-derived ocular cells focus on a single, tissue-specific lineage, however, that faces difficulty in reflecting the complexity of eye development. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0176
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http://dx.doi.org/10.1089/scd.2018.0176DOI Listing
April 2019
8 Reads

Mechanisms of the Immunomodulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Facial Nerve Injury in Sprague-Dawley Rats.

Stem Cells Dev 2019 Apr 26;28(7):489-496. Epub 2019 Feb 26.

4 Department of Cell Biology, Center of Excellence in Tissue Engineering, Key Laboratory of Beijing, Institute of Basic Medical Sciences and School of Basic Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Normal facial nerve (FN) function is very important for human being. However, if injured, FN function is difficult to restore completely. Recently, many studies reported the immune regulation function of stem cells (SCs). Read More

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http://dx.doi.org/10.1089/scd.2018.0104DOI Listing
April 2019
1 Read

Combinations of Growth Factors Regulating LIF/STAT3, WNT, and FGF2 Pathways Sustain Pluripotency-Related Proteins in Cat Embryonic Cells.

Stem Cells Dev 2019 Mar 30;28(5):329-340. Epub 2019 Jan 30.

2 Center for Species Survival, Smithsonian Conservation Biology Institute (SCBI), Washington, District of Columbia.

Propagation of pluripotent cells from early stage embryos in mouse and human highly depend on leukemia inhibitory factor (LIF)/signal transducer and activator of transcription 3 (STAT3) and FGF2/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathways. However, mechanisms for maintaining pluripotency in embryonic stem cells using various combinations of growth factors (targeting LIF or FGF2 pathways) and inhibitors (targeting WNT/GSK3 or FGF2 pathways) still have to be deciphered in other models, including the domestic cat. Our objective was to understand how cytokines influence pluripotency in the cat inner cell mass (ICM) outgrowths. Read More

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http://dx.doi.org/10.1089/scd.2018.0109DOI Listing

Mesenchymal Stromal Cells Derived from Normal Cervix and Cervical Cancer Tumors Increase CD73 Expression in Cervical Cancer Cells Through TGF-β1 Production.

Stem Cells Dev 2019 Apr 5;28(7):477-488. Epub 2019 Mar 5.

1 Laboratorio de Inmunología y Cáncer, Unidad de Investigación Médica en Enfermedades Oncológicas, CMN SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.

Mesenchymal stromal cells (MSCs) in the tumor microenvironment (TME) participate together with tumor cells to suppress antitumor effector cells through the production of immunosuppressive factors, such as transforming growth factor-beta 1 (TGF-β1). Furthermore, TGF-β1 can induce 5'-nucleotidase (CD73) expression in various cell types; this functional activity is associated with the production of adenosine (Ado), which is an immunosuppressive nucleoside. In this study, we provide evidence that coculture of MSCs derived from cervical tumors (CeCa-MSC) with CeCa tumor cells increases CD73 expression in tumor cells and the capacity of these cells to generate Ado in a MSC ratio-dependent manner. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0183
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http://dx.doi.org/10.1089/scd.2018.0183DOI Listing
April 2019
4 Reads

Behavior of Stem-Like Cells, Precursors for Tissue Regeneration in Urodela, Under Conditions of Microgravity.

Stem Cells Dev 2019 Apr 28;28(7):423-437. Epub 2019 Feb 28.

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.

We summarize data from our experiments on stem-like cell-dependent regeneration in amphibians in microgravity. Considering its deleterious effect on many tissues, we asked whether microgravity is compatible with reparative processes, specifically activation and proliferation of source cells. Experiments were conducted using tailed amphibians, which combine profound regenerative capabilities with high robustness, allowing an in vivo study of lens, retina, limb, and tail regeneration in challenging settings of spaceflight. Read More

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http://dx.doi.org/10.1089/scd.2018.0220DOI Listing

A Free Radical Scavenger Ameliorates Teratogenic Activity of a DNA Hypomethylating Hematological Therapeutic.

Stem Cells Dev 2019 Mar 11. Epub 2019 Mar 11.

2 Center of Excellence in Reproductive and Regenerative Medicine, School of Medicine, Zagreb, Croatia.

The spin-trap free radical scavenger N-tert-butyl-α-phenylnitron (PBN) ameliorated effects of several teratogens involving reactive oxygen species (ROS). We investigated for the first time whether PBN could ameliorate teratogenesis induced by a DNA hypomethylating hematological therapeutic 5-azacytidine (5azaC). At days 12 and 13 of gestation, Fisher rat dams were pretreated by an i. Read More

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http://dx.doi.org/10.1089/scd.2018.0194DOI Listing
March 2019
2 Reads

A SMN2 Splicing Modifier Rescues the Disease Phenotypes in an In Vitro Human Spinal Muscular Atrophy Model.

Stem Cells Dev 2019 Apr 20;28(7):438-453. Epub 2019 Feb 20.

1 Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

Spinal muscular atrophy (SMA) is caused by the mutation or deletion of the survival motor neuron 1 (SMN1) gene. Only ∼10% of the products of SMN2, a paralogue of SMN1, are functional full-length SMN (SMN-FL) proteins, whereas SMN2 primarily produces alternatively spliced transcripts lacking exon 7. Reduced SMN protein levels in SMA patients lead to progressive degeneration of spinal motor neurons (MNs). Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0181
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http://dx.doi.org/10.1089/scd.2018.0181DOI Listing
April 2019
16 Reads

Cerebral Cortex Generated from Pluripotent Stem Cells to Model Corticogenesis and Rebuild Cortical Circuits: In Vitro Veritas?

Stem Cells Dev 2019 Mar 20;28(6):361-369. Epub 2019 Feb 20.

Institut de Génomique Fonctionnelle, IGF, CNRS, INSERM, Université de Montpellier, Montpellier, France.

Organoids and cells generated in vitro from pluripotent stem cells (PSCs) are considered to be robust models of development and a conceivable source of transplants for putative cell therapy. However, a fundamental question about organoids and cells generated from PSCs is as follows: do they faithfully reproduce the in vivo tissue they are supposed to mimic and replace? This question is particularly relevant to complex tissues such as the cerebral cortex. In this review, we have tackled this issue by comparing cerebral cortices generated in vitro from PSCs to the in vivo cortex, with a particular focus on their respective cellular composition, molecular and epigenetic signatures, and brain connectivity. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0233
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http://dx.doi.org/10.1089/scd.2018.0233DOI Listing
March 2019
11 Reads

Spaceflight Promoted Myocardial Differentiation of Induced Pluripotent Stem Cells: Results from Tianzhou-1 Space Mission.

Stem Cells Dev 2019 Mar 25;28(6):357-360. Epub 2019 Feb 25.

1 Tissue Engineering Research Center, Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing, China.

During space travel, exposure to microgravity may have profound influence on the physiological function of mammalian cells. In this study, we took opportunity of the Tianzhou-1 (TZ-1) mission to investigate how spaceflight may affect cardiac differentiation of mouse induced pluripotent stem cells (iPSCs). A bioreactor was engineered to perform cell culturing and the time-lapse imaging experiments on-orbit. Read More

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http://dx.doi.org/10.1089/scd.2018.0240DOI Listing
March 2019
8 Reads

Identification of a Novel Transcription Factor Required for Osteogenic Differentiation of Mesenchymal Stem Cells.

Stem Cells Dev 2019 Mar 28;28(6):370-383. Epub 2019 Feb 28.

1 CEINGE-Biotecnologie Avanzate, Naples, Italy.

Osteogenic differentiation is a complex and still poorly understood biological process regulated by intrinsic cellular signals and extrinsic microenvironmental cues. Following appropriate stimuli, mesenchymal stem cells (MSCs) differentiate into osteoblasts through a tightly regulated multistep process driven by several transcription factors and characterized by the expression of a number of bone-specific proteins. In this study, we describe a novel transcription factor that we named osteoblast inducer (ObI)-1, involved in MSC differentiation toward the osteogenic lineage. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0152
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http://dx.doi.org/10.1089/scd.2018.0152DOI Listing
March 2019
13 Reads

Activin Receptor 2 Antagonization Impairs Adipogenic and Enhances Osteogenic Differentiation in Mouse Adipose-Derived Stem Cells and Mouse Bone Marrow-Derived Stem Cells In Vitro and In Vivo.

Stem Cells Dev 2019 Mar 20;28(6):384-397. Epub 2019 Feb 20.

Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany.

Tumors, traumata, burn injuries or surgeries can lead to critical-sized bony defects which need to be reconstructed. Mesenchymal stem cells (MSCs) have the ability to differentiate into multiple cell lineages and thus present a promising alternative for use in tissue engineering and reconstruction. However, there is an ongoing debate whether all MSCs are equivalent in their differentiation and proliferation ability. Read More

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http://dx.doi.org/10.1089/scd.2018.0155DOI Listing
March 2019
1 Read

Exosomes Derived from Human Primed Mesenchymal Stem Cells Induce Mitosis and Potentiate Growth Factor Secretion.

Stem Cells Dev 2019 Mar 26;28(6):398-409. Epub 2019 Feb 26.

1 Department of Otolaryngology, University of California, Davis, Davis, California.

Mesenchymal stem cells (MSCs) facilitate functional recovery in numerous animal models of inflammatory and ischemic tissue-related diseases with a growing body of research suggesting that exosomes mediate many of these therapeutic effects. It remains unclear, however, which types of proteins are packaged into exosomes compared with the cells from which they are derived. In this study, using comprehensive proteomic analysis, we demonstrated that human primed MSCs secrete exosomes (pMEX) that are packaged with markedly higher fractions of specific protein subclasses compared with their cells of origin, indicating regulation of their contents. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0200
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http://dx.doi.org/10.1089/scd.2018.0200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441283PMC
March 2019
7 Reads

Equine Allogeneic Chondrogenic Induced Mesenchymal Stem Cells Are an Effective Treatment for Degenerative Joint Disease in Horses.

Stem Cells Dev 2019 Mar 13;28(6):410-422. Epub 2019 Feb 13.

1 Global Stem cell Technology NV, Anacura Group, Evergem, Belgium.

Degenerative joint disease is one of the main causes of equine early retirement from pleasure riding or a performance career. The disease is initially triggered by an abnormal loading of normal cartilage or a normal loading of abnormal cartilage. This primary insult is accompanied with joint inflammation, which leads to further progressive degeneration of the articular cartilage and changes in the surrounding tissues. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0061
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http://dx.doi.org/10.1089/scd.2018.0061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441287PMC
March 2019
9 Reads

Human Fetal Cardiac Mesenchymal Stromal Cells Differentiate In Vivo into Endothelial Cells and Contribute to Vasculogenesis in Immunocompetent Mice.

Stem Cells Dev 2019 Mar 5;28(5):310-318. Epub 2019 Feb 5.

1 Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.

Mesenchymal stromal cells (MSCs) have shown great potential as a treatment for systemic inflammatory diseases, but their local regenerative properties are highly tissue- and site specific. Previous studies have demonstrated that adult human MSCs respond to inflammatory cytokines through the release of paracrine factors that stimulate angiogenesis, but they do not themselves differentiate into vascular structures in vivo. In this study, we used human fetal cardiac MSCs (hfcMSCs) harvested during the first trimester of heart development and injected them into the subcutaneous tissue of normal immunocompetent mice treated with short-term costimulation blockade for tolerance induction. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0198
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http://dx.doi.org/10.1089/scd.2018.0198DOI Listing
March 2019
13 Reads

Attempts for Generation of Embryonic Stem Cells from Human Embryos Following In Vitro Embryo Twinning.

Stem Cells Dev 2019 Mar 31;28(5):303-309. Epub 2019 Jan 31.

1 Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

In vitro embryo twinning can be used to increase the number of the human embryos available for production of human embryonic stem cell (hESC) lines. The aim of this study was to generate hESCs following the production of the twin embryos by in vitro embryo splitting procedures. In total 21 chromosomally abnormal (three pronuclei) embryos underwent in vitro embryo twinning and were allowed to develop to the blastocyst stage. Read More

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http://dx.doi.org/10.1089/scd.2018.0168DOI Listing
March 2019
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Diabetic Human Adipose-Derived Stem Cells Accelerate Pressure Ulcer Healing by Inducing Angiogenesis and Neurogenesis.

Stem Cells Dev 2019 Mar 4;28(5):319-328. Epub 2019 Feb 4.

Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Adipose-derived stem cells (ASCs) possess a well-characterized capacity to enhance cutaneous wound healing. However, many controversies exist regarding ASCs from diabetic patients (dASCs). No report exists on the administration of dASCs for the treatment of pressure ulcers. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0245
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http://dx.doi.org/10.1089/scd.2018.0245DOI Listing
March 2019
5 Reads

BCR-Abl Silencing by Short Interfering RNA: A Potent Approach to Sensitize Chronic Myeloid Leukemia Cells to Tyrosine Kinase Inhibitor Therapy.

Stem Cells Dev 2019 Jan 24. Epub 2019 Jan 24.

1 Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Canada.

Nonviral gene therapy with specific short interfering RNAs (siRNAs) against BCR-Abl can be an alternative and/or supportive therapy of chronic myeloid leukemia (CML) with tyrosine kinase inhibitors (TKIs), given the often observed resistance to TKIs in clinical setting. In this study, we explored the feasibility of BCR-Abl siRNA therapy in CML K562 cells in vitro by employing a cationic polymer derived from cholesterol (Chol) grafted low-molecular weight polyethyleneimine (PEI). The first generation TKI imatinib upregulated the expression of BCR-Abl in K562 cells as expected. Read More

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http://dx.doi.org/10.1089/scd.2018.0196DOI Listing
January 2019
6 Reads

Breast Tumor Microenvironment Can Transform Naive Mesenchymal Stem Cells into Tumor-Forming Cells in Nude Mice.

Stem Cells Dev 2019 Mar;28(5):341-352

1 Applied Stem Cell Laboratory, Heart and Vascular Institute, Department of Medicine, Tulane University Health Science Center, New Orleans, Louisiana.

How mesenchymal stem cells (MSCs) interact with tumor cells and promote tumor growth is not well understood. In this study, we demonstrate that when naive MSCs and malignant breast cancer cells (MDA-MB231) were injected into opposing mammary glands of an immunodeficient nude mouse, both cell types formed tumor-like masses within 8 weeks at the injected site. Surprisingly, MDA-MB231 cells were detected in the opposing mammary gland injected with the naive MSCs, indicating migration and crosstalk between naive MSCs and MDA-MB231 cells. Read More

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http://dx.doi.org/10.1089/scd.2018.0110DOI Listing
March 2019
1 Read

Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.

Stem Cells Dev 2019 Feb 28;28(4):278-289. Epub 2019 Jan 28.

1 Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York.

There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP). Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers. Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells. Read More

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http://dx.doi.org/10.1089/scd.2018.0197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389772PMC
February 2019
1 Read

Compromised Antibacterial Function of Multipotent Stromal Cells in Diabetes.

Stem Cells Dev 2019 Feb 22;28(4):268-277. Epub 2019 Jan 22.

Orthobiologic Laboratory, MedStar Union Memorial Hospital, Baltimore, Maryland.

In diabetes, multipotent stromal cells (MSCs) are functionally deficient. It is unknown, however, whether their antibacterial function is compromised. In this study, MSCs were isolated from the bone marrow samples provided by nine diabetic and six nondiabetic donors and treated with or without Escherichia coli lipopolysaccharides (LPS). Read More

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http://dx.doi.org/10.1089/scd.2018.0219DOI Listing
February 2019
2 Reads

Transplantation of Retinal Progenitor Cells from Optic Cup-Like Structures Differentiated from Human Embryonic Stem Cells In Vitro and In Vivo Generation of Retinal Ganglion-Like Cells.

Stem Cells Dev 2019 Feb;28(4):258-267

2 Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Human embryonic stem cells (hESCs) have the potential to differentiate along the retinal lineage. We have efficiently differentiated human pluripotent stem cells into optic cup-like structures by using a novel retinal differentiation medium (RDM). The purpose of this study was to determine whether the retinal progenitor cells (RPCs) derived from hESCs can integrate into the host retina and differentiate into retinal ganglion cells (RGCs) in vivo. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0076
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http://dx.doi.org/10.1089/scd.2018.0076DOI Listing
February 2019
25 Reads
3.727 Impact Factor

The Differentiation Potency of Trophoblast Stem Cells from Mouse Androgenetic Embryos.

Stem Cells Dev 2019 Feb 14;28(4):290-302. Epub 2019 Jan 14.

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

In mice, trophoblast stem (TS) cells are derived from the polar trophectoderm of blastocysts. TS cells cultured in the presence of fibroblast growth factor 4 (Fgf4) are in an undifferentiated state and express undifferentiated marker genes such as Cdx2. After removing Fgf4 from the culture medium, TS cells drastically reduce the expression of undifferentiated marker genes, stop cell proliferation, and differentiate into all trophoblast cell subtypes. Read More

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http://dx.doi.org/10.1089/scd.2018.0068DOI Listing
February 2019
2 Reads