2,669 results match your criteria Stem Cells and Development [Journal]


Induction of mouse peritoneum mesenchymal stem cells into germ cell-like cells using follicular fluid and cumulus cells conditioned media.

Stem Cells Dev 2019 Feb 15. Epub 2019 Feb 15.

Royan Institute, 48499, Embryology, Tehran, Iran (the Islamic Republic of) ;

The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell like cells using human follicular fluid (HFF) and human cumulus conditioned medium (HCCM). Anterior abdominal wall and intestinal peritoneum explants were used for cells isolation and cultured in DMEM. Read More

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http://dx.doi.org/10.1089/scd.2018.0149DOI Listing
February 2019

Isolation and characterization of cerebellum-derived stem cells in post-stroke human brain.

Stem Cells Dev 2019 Feb 15. Epub 2019 Feb 15.

Hyogo College of Medicine, 12818, Nishinomiya, Hyogo, Japan ;

There is compelling evidence that the mature central nervous system (CNS) harbors stem cell populations outside conventional neurogenic regions. We previously demonstrated that brain pericytes (PCs) in both mouse and human exhibit multipotency to differentiate into various neural lineages following cerebral ischemia. Pericytes are found throughout the CNS, including cerebellum, but it remains unclear whether cerebellar PCs also form ischemia-induced multipotent stem cells (iSCs). Read More

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http://dx.doi.org/10.1089/scd.2018.0232DOI Listing
February 2019

Biodistribution of glial progenitors in a 3D printed model of the piglet cerebral ventricular system.

Stem Cells Dev 2019 Feb 14. Epub 2019 Feb 14.

Mossakowski Medical Research Centre, Polish Academy of Sciences, NeuroRepair Department, Warsaw, Mazowsze, Poland.

White matter damage persists in hypoxic-ischemic newborns even when treated with hypothermia. We have previously shown that intra-ventricular delivery of human glial progenitors (GPs) at the neonatal stage is capable of replacing abnormal host glia and rescuing the lifespan of dysmyelinated mice. However, such transplantation in the human brain poses significant challenges as related to high-volume ventricles and long cell migration distances. Read More

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http://dx.doi.org/10.1089/scd.2018.0172DOI Listing
February 2019

Proteomic analysis reveals temporal changes in protein expression in human induced pluripotent stem cell-derived cardiomyocytes in vitro.

Stem Cells Dev 2019 Feb 13. Epub 2019 Feb 13.

Imperial College, NHLI , Dovehouse St , London, United Kingdom of Great Britain and Northern Ireland , SW3 6LY ;

Human induced pluripotent stem cell-derived cardiomyocytes hold great promise for regenerative medicine and in vitro screening. Despite displaying key cardiomyocyte phenotypic characteristics, they more closely resemble foetal/neonatal cardiomyocytes and further characterisation is necessary. Combining the use of tandem mass tags to label cell lysates, followed by multiplexing, we have determined the effects of short term (30 day) in vitro culture on human induced pluripotent stem cell derived cardiomyocyte protein expression. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0210
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http://dx.doi.org/10.1089/scd.2018.0210DOI Listing
February 2019
3 Reads

Comparison of cultured human cardiomyocyte clusters obtained from embryos/fetuses or derived from human embryonic stem cells.

Stem Cells Dev 2019 Feb 13. Epub 2019 Feb 13.

University Hospital of Copenhagen, Laboratory of Reproductive Biology, Copenhagen, Denmark ;

Cardiomyocytes (CM) derived from human embryonic stem cells (hESC) or induced pluripotent stem cells (iPSC) are used to study cardiogenesis and mechanisms of heart disease and are advancing as a method for toxicological screening of drugs. The phenotype of stem cell-derived CMs should ideally resemble native CMs. Here we compare embryonic/fetal CMs with hESC-derived CMs according to function and morphology. Read More

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http://dx.doi.org/10.1089/scd.2018.0231DOI Listing
February 2019

Human bone marrow mesenchymal stem cells functionalized by hybrid baculovirus-adeno-associated viral vectors for targeting hypopharyngeal carcinoma.

Stem Cells Dev 2019 Feb 12. Epub 2019 Feb 12.

Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Department of Otolaryngology , No. 197, Ruijin 2nd Road , Shanghai, China , 200025 ;

Hypopharyngeal carcinoma is a common malignant tumor of the head and neck with a very poor prognosis; the median survival time for curatively treated patients was 17.2 months in India. However, cell-based gene therapy holds promise to improve patient outcomes. Read More

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http://dx.doi.org/10.1089/scd.2018.0252DOI Listing
February 2019
2 Reads

Meta-analysis of the safety and efficacy of stem cell therapies for ischemic stroke in preclinical and clinical studies.

Stem Cells Dev 2019 Feb 11. Epub 2019 Feb 11.

Southern Medical University, 70570, Zhujiang Hospital, Guangzhou, Guangdong, China ;

Recent studies have indicated that stem cell transplantation may be effective in the treatment of ischemic stroke. Therefore, we performed a meta-analysis to evaluate the safety and efficacy of stem cell therapy for ischemic stroke in preclinical and clinical studies. In accordance with PRISMA guidelines, we searched the PubMed, Cochrane Library, Embase, Web of science, and Ovid databases from inception through May 2018. Read More

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http://dx.doi.org/10.1089/scd.2018.0218DOI Listing
February 2019
1 Read

Effects of gastric cancer cell-derived exosomes on the immune regulation of mesenchymal stem cells by the NF-kB signaling pathway.

Stem Cells Dev 2019 Feb 4. Epub 2019 Feb 4.

Chinese Academy of Medical Science and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine , 5# Dongdansantiao Beijing 100005. PR China , Beijing, Beijing, China , 100005 ;

Mesenchymal stem cells (MSCs) are an important component of the tumor microenvironment, which play an important role in tumor development. Exosomes derived from tumor cells can affect the biological characteristics of MSCs. Our study examined the effects of exosomes derived from gastric cancer cells on MSC immunomodulatory functions. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0125
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http://dx.doi.org/10.1089/scd.2018.0125DOI Listing
February 2019
1 Read

Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.

Stem Cells Dev 2019 Feb 2. Epub 2019 Feb 2.

Shandong Eye Institute, 92297, Shandong Provincial Key Laboratory of Ophthalmology, Qingdao, China ;

Eye is a complex organ with highly specialized tissue structure. The establishment of human pluripotent stem cells (hPSCs) has allowed the simulation of eye development in vitro. Most differentiation works of hPSC-derived ocular cells focus on a single, tissue-specific lineage, however, which face the difficulty in reflecting the complexity of eye development. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0176
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http://dx.doi.org/10.1089/scd.2018.0176DOI Listing
February 2019
3 Reads

Mechanisms of the immunomodulation effects of bone marrow-derived mesenchymal stem cells on facial nerve injury in SD rats.

Stem Cells Dev 2019 Feb 1. Epub 2019 Feb 1.

Chinese Academy of Medical Science and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine , 5# Dongdansantiao Beijing 100005. PR China , Beijing, Beijing, China , 100005 ;

Normal facial nerve (FN) function is very important for human being. However, if injured, FN function is difficult to restore completely. Recently, many studies reported the immune regulation function of stem cells (SCs). Read More

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http://dx.doi.org/10.1089/scd.2018.0104DOI Listing
February 2019
1 Read

Combinations of Growth Factors Regulating LIF/STAT3, WNT, and FGF2 Pathways Sustain Pluripotency-Related Proteins in Cat Embryonic Cells.

Stem Cells Dev 2019 Jan 30. Epub 2019 Jan 30.

2 Center for Species Survival, Smithsonian Conservation Biology Institute (SCBI), Washington, District of Columbia.

Propagation of pluripotent cells from early stage embryos in mouse and human highly depend on leukemia inhibitory factor (LIF)/signal transducer and activator of transcription 3 (STAT3) and FGF2/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathways. However, mechanisms for maintaining pluripotency in embryonic stem cells using various combinations of growth factors (targeting LIF or FGF2 pathways) and inhibitors (targeting WNT/GSK3 or FGF2 pathways) still have to be deciphered in other models, including the domestic cat. Our objective was to understand how cytokines influence pluripotency in the cat inner cell mass (ICM) outgrowths. Read More

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http://dx.doi.org/10.1089/scd.2018.0109DOI Listing
January 2019

Mesenchymal stromal cells derived from normal cervix and cervical cancer tumors increase CD73 expression in cervical cancer cells through TGF-β1 production.

Stem Cells Dev 2019 Jan 30. Epub 2019 Jan 30.

Instituto Mexicano del Seguro Social, Laboratorio de Inmunología y Cáncer. UIMEO, H Oncología, CMN SXXI, Ciudad de México, Mexico.

Mesenchymal stromal cells (MSCs) in the tumor microenvironment (TME) participate together with tumor cells to suppress antitumor effector cells through the production of immunosuppressive factors such as TGF-β1. Furthermore, TGF-β1 can induce 5'-nucleotidase (CD73) expression in various cell types; this functional activity is associated with the production of adenosine (Ado), which is an immunosuppressive nucleoside. In this study, we provide evidence that coculture of MSCs derived from cervical tumors (CeCa-MSC) with CeCa tumor cells increases CD73 expression in tumor cells and the capacity of these cells to generate Ado in a MSC-ratio-dependent manner. Read More

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http://dx.doi.org/10.1089/scd.2018.0183DOI Listing
January 2019

Behavior of stem-like cells, precursors for tissue regeneration in Urodela, under conditions of microgravity.

Stem Cells Dev 2019 Jan 30. Epub 2019 Jan 30.

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Laboratory of Regeneration, Moscow ;

We summarize data from our experiments on stem-like cell dependent regeneration in amphibians in microgravity. Considering its deleterious effect on many tissues, we asked whether microgravity is compatible with reparative processes, specifically activation and proliferation of source cells. Experiments were conducted using tailed amphibians, which combine profound regenerative capabilities with high robustness, allowing in vivo study of lens, retina, limb and tail regeneration in challenging settings of spaceflight. Read More

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http://dx.doi.org/10.1089/scd.2018.0220DOI Listing
January 2019

A Free Radical Scavenger Ameliorates Teratogenic Activity of a DNA Hypomethylating Hematological Therapeutic.

Stem Cells Dev 2019 Jan 23. Epub 2019 Jan 23.

University of Zagreb, School of Medicine, Department of Medical Biology, Zagreb, Croatia.

The spin-trap free radical scavenger N-tert-butyl-α-phenylnitron (PBN) ameliorated effects of several teratogens involving reactive oxygen species. We investigated for the first time whether PBN could ameliorate teratogenesis induced by a DNA hypomethylating hematological therapeutic 5-azacytidine (5azaC). At day 12 and 13 day of gestation Fisher rat dams were pretreated by i. Read More

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http://dx.doi.org/10.1089/scd.2018.0194DOI Listing
January 2019
1 Read

A SMN2 Splicing Modifier Rescues the Disease Phenotypes in an In Vitro Human Spinal Muscular Atrophy Model.

Stem Cells Dev 2019 Feb 20. Epub 2019 Feb 20.

1 Stem Cell Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

Spinal muscular atrophy (SMA) is caused by the mutation or deletion of the survival motor neuron 1 (SMN1) gene. Only ∼10% of the products of SMN2, a paralogue of SMN1, are functional full-length SMN (SMN-FL) proteins, whereas SMN2 primarily produces alternatively spliced transcripts lacking exon 7. Reduced SMN protein levels in SMA patients lead to progressive degeneration of spinal motor neurons (MNs). Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0181
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http://dx.doi.org/10.1089/scd.2018.0181DOI Listing
February 2019
6 Reads

Cerebral Cortex Generated from Pluripotent Stem Cells to Model Corticogenesis and Rebuild Cortical Circuits: In Vitro Veritas?

Stem Cells Dev 2019 Feb 20. Epub 2019 Feb 20.

Institut de Génomique Fonctionnelle, IGF, CNRS, INSERM, Université de Montpellier, Montpellier, France.

Organoids and cells generated in vitro from pluripotent stem cells (PSCs) are considered to be robust models of development and a conceivable source of transplants for putative cell therapy. However, a fundamental question about organoids and cells generated from PSCs is as follows: do they faithfully reproduce the in vivo tissue they are supposed to mimic and replace? This question is particularly relevant to complex tissues such as the cerebral cortex. In this review, we have tackled this issue by comparing cerebral cortices generated in vitro from PSCs to the in vivo cortex, with a particular focus on their respective cellular composition, molecular and epigenetic signatures, and brain connectivity. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0233
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http://dx.doi.org/10.1089/scd.2018.0233DOI Listing
February 2019
5 Reads

Spaceflight Promoted Myocardial Differentiation of Induced Pluripotent Stem Cells - Results from Tianzhou-1 Mission.

Stem Cells Dev 2019 Jan 18. Epub 2019 Jan 18.

Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing, Beijing, China ;

During space travel, exposure to microgravity may have profound influence on the physiological function of mammalian cells. In this study, we took opportunity of the TZ-1 mission to investigate how spaceflight may affect cardiac differentiation of mouse induced pluripotent stem cells (iPSCs). A bioreactor was engineered to perform cell culture and the time-lapse imaging experiments on-obit. Read More

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http://dx.doi.org/10.1089/scd.2018.0240DOI Listing
January 2019
2 Reads

Identification of a Novel Transcription Factor Required For Osteogenic Differentiation Of Mesenchymal Stem Cells.

Stem Cells Dev 2019 Jan 18. Epub 2019 Jan 18.

Università degli Studi di Napoli Federico II, Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Naples, Italy.

Osteogenic differentiation is a complex and still poorly understood biological process regulated by intrinsic cellular signals and extrinsic micro-environmental cues. Following appropriate stimuli, mesenchymal stem cells (MSCs) differentiate into osteoblasts through a tightly regulated multi-step process driven by several transcription factors and characterized by the expression of a number of bone-specific proteins. Here, we describe a novel transcription factor that we named Osteoblast Inducer (ObI)-1, involved in MSC differentiation towards the osteogenic lineage. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0152
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http://dx.doi.org/10.1089/scd.2018.0152DOI Listing
January 2019
7 Reads

Activin Receptor 2 Antagonization Impairs Adipogenic and Enhances Osteogenic Differentiation in Mouse Adipose-Derived Stem Cells and Mouse Bone Marrow-Derived Stem Cells In Vitro and In Vivo.

Stem Cells Dev 2019 Feb 20. Epub 2019 Feb 20.

Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany.

Tumors, traumata, burn injuries or surgeries can lead to critical-sized bony defects which need to be reconstructed. Mesenchymal stem cells (MSCs) have the ability to differentiate into multiple cell lineages and thus present a promising alternative for use in tissue engineering and reconstruction. However, there is an ongoing debate whether all MSCs are equivalent in their differentiation and proliferation ability. Read More

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http://dx.doi.org/10.1089/scd.2018.0155DOI Listing
February 2019
1 Read

Exosomes Derived from Human Primed Mesenchymal Stem Cells Induce Mitosis and Potentiate Growth Factor Secretion.

Stem Cells Dev 2019 Jan 14. Epub 2019 Jan 14.

University of California Davis Department of Internal Medicine, 158565, Stem Cell Program , 2129 Stockton Blvd , Room 1300 , Sacramento, California, United States , 95817-1460 ;

Mesenchymal stem cells (MSCs) facilitate functional recovery in numerous animal models of inflammatory and ischemic tissue related diseases with a growing body of research suggesting that exosomes mediate many of these therapeutic effects. It remains unclear, however, which types of proteins are packaged into exosomes as compared to the cells from which they are derived. Here, using comprehensive proteomic analysis, we demonstrated that human primed MSCs secrete exosomes (pMEX) that are packaged with markedly higher fractions of specific protein subclasses as compared to their cells of origin, indicating regulation of their contents. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0200
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http://dx.doi.org/10.1089/scd.2018.0200DOI Listing
January 2019
4 Reads

Equine Allogeneic Chondrogenic Induced Mesenchymal Stem Cells Are an Effective Treatment for Degenerative Joint Disease in Horses.

Stem Cells Dev 2019 Feb 13. Epub 2019 Feb 13.

1 Global Stem cell Technology NV, Anacura Group, Evergem, Belgium.

Degenerative joint disease is one of the main causes of equine early retirement from pleasure riding or a performance career. The disease is initially triggered by an abnormal loading of normal cartilage or a normal loading of abnormal cartilage. This primary insult is accompanied with joint inflammation, which leads to further progressive degeneration of the articular cartilage and changes in the surrounding tissues. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0061
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http://dx.doi.org/10.1089/scd.2018.0061DOI Listing
February 2019
3 Reads

Human Fetal Cardiac Mesenchymal Stromal Cells Differentiate In Vivo into Endothelial Cells and Contribute to Vasculogenesis in Immunocompetent Mice.

Stem Cells Dev 2019 Feb 5. Epub 2019 Feb 5.

1 Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.

Mesenchymal stromal cells (MSCs) have shown great potential as a treatment for systemic inflammatory diseases, but their local regenerative properties are highly tissue- and site specific. Previous studies have demonstrated that adult human MSCs respond to inflammatory cytokines through the release of paracrine factors that stimulate angiogenesis, but they do not themselves differentiate into vascular structures in vivo. In this study, we used human fetal cardiac MSCs (hfcMSCs) harvested during the first trimester of heart development and injected them into the subcutaneous tissue of normal immunocompetent mice treated with short-term costimulation blockade for tolerance induction. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0198
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http://dx.doi.org/10.1089/scd.2018.0198DOI Listing
February 2019
10 Reads

Attempts for Generation of Embryonic Stem Cells from Human Embryos Following In Vitro Embryo Twinning.

Stem Cells Dev 2019 Jan 31. Epub 2019 Jan 31.

1 Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences , Yazd, Iran .

In vitro embryo twinning can be used to increase the number of the human embryos available for production of human embryonic stem cell (hESC) lines. The aim of this study was to generate hESCs following the production of the twin embryos by in vitro embryo splitting procedures. In total 21 chromosomally abnormal (three pronuclei) embryos underwent in vitro embryo twinning and were allowed to develop to the blastocyst stage. Read More

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http://dx.doi.org/10.1089/scd.2018.0168DOI Listing
January 2019
1 Read

Diabetic Human Adipose-Derived Stem Cells Accelerate Pressure Ulcer Healing by Inducing Angiogenesis and Neurogenesis.

Stem Cells Dev 2019 Feb 4. Epub 2019 Feb 4.

Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.

Adipose-derived stem cells (ASCs) possess a well-characterized capacity to enhance cutaneous wound healing. However, many controversies exist regarding ASCs from diabetic patients (dASCs). No report exists on the administration of dASCs for the treatment of pressure ulcers. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0245
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http://dx.doi.org/10.1089/scd.2018.0245DOI Listing
February 2019
5 Reads

BCR-Abl Silencing by Short Interfering RNA: A Potent Approach to Sensitize Chronic Myeloid Leukemia Cells to Tyrosine Kinase Inhibitor Therapy.

Stem Cells Dev 2019 Jan 24. Epub 2019 Jan 24.

1 Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, Canada.

Nonviral gene therapy with specific short interfering RNAs (siRNAs) against BCR-Abl can be an alternative and/or supportive therapy of chronic myeloid leukemia (CML) with tyrosine kinase inhibitors (TKIs), given the often observed resistance to TKIs in clinical setting. In this study, we explored the feasibility of BCR-Abl siRNA therapy in CML K562 cells in vitro by employing a cationic polymer derived from cholesterol (Chol) grafted low-molecular weight polyethyleneimine (PEI). The first generation TKI imatinib upregulated the expression of BCR-Abl in K562 cells as expected. Read More

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http://dx.doi.org/10.1089/scd.2018.0196DOI Listing
January 2019
5 Reads

Breast Tumor Microenvironment Can Transform Naïve Mesenchymal Stem Cells into Tumor Forming Cells in Nude Mice.

Stem Cells Dev 2018 Dec 20. Epub 2018 Dec 20.

Tulane University, Medicine , 1430 Tulane Avenue , New Orleans, Louisiana, United States , 70112 ;

How mesenchymal stem cells (MSCs) interact with tumor cells and promote tumor growth is not well understood. Here, we demonstrate that when naïve MSCs and malignant breast cancer cells (MDA-MB231) were injected into opposing mammary glands of an immunodeficient nude mouse, both cell types formed tumor-like masses within 8 weeks at the injected site. Surprisingly, MDA-MB231 cells were detected in the opposing mammary gland injected with the naïve MSCs, indicating migration and crosstalk between naïve MSCs and MDA-MB231 cells. Read More

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http://dx.doi.org/10.1089/scd.2018.0110DOI Listing
December 2018
1 Read

Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells Toward Hepatocytes.

Stem Cells Dev 2019 Feb 28;28(4):278-289. Epub 2019 Jan 28.

1 Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York.

There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP). Currently, the vast majority of primary hHEP are obtained from human tissue through cadavers. Recent advances in stem cell differentiation have opened up the possibility to obtain fully functional hepatocytes from embryonic or induced pluripotent stem cells, or adult stem cells. Read More

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http://dx.doi.org/10.1089/scd.2018.0197DOI Listing
February 2019
1 Read

Clonal Analysis of Mouse Mammary Luminal Epithelial Cell Transplants.

Stem Cells Dev 2019 Jan 31. Epub 2019 Jan 31.

1 Terry Fox Laboratory, British Columbia Cancer Agency , Vancouver, Canada .

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https://www.liebertpub.com/doi/10.1089/scd.2018.0158
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January 2019
7 Reads

Compromised Antibacterial Function of Multipotent Stromal Cells in Diabetes.

Stem Cells Dev 2019 Feb 22;28(4):268-277. Epub 2019 Jan 22.

Orthobiologic Laboratory, MedStar Union Memorial Hospital, Baltimore, Maryland.

In diabetes, multipotent stromal cells (MSCs) are functionally deficient. It is unknown, however, whether their antibacterial function is compromised. In this study, MSCs were isolated from the bone marrow samples provided by nine diabetic and six nondiabetic donors and treated with or without Escherichia coli lipopolysaccharides (LPS). Read More

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http://dx.doi.org/10.1089/scd.2018.0219DOI Listing
February 2019
2 Reads

Transplantation of Retinal Progenitor Cells from Optic Cup-Like Structures Differentiated from Human Embryonic Stem Cells In Vitro and In Vivo Generation of Retinal Ganglion-Like Cells.

Stem Cells Dev 2019 Feb;28(4):258-267

2 Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Human embryonic stem cells (hESCs) have the potential to differentiate along the retinal lineage. We have efficiently differentiated human pluripotent stem cells into optic cup-like structures by using a novel retinal differentiation medium (RDM). The purpose of this study was to determine whether the retinal progenitor cells (RPCs) derived from hESCs can integrate into the host retina and differentiate into retinal ganglion cells (RGCs) in vivo. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0076
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http://dx.doi.org/10.1089/scd.2018.0076DOI Listing
February 2019
13 Reads

The Differentiation Potency of Trophoblast Stem Cells from Mouse Androgenetic Embryos.

Stem Cells Dev 2019 Feb 14;28(4):290-302. Epub 2019 Jan 14.

Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

In mice, trophoblast stem (TS) cells are derived from the polar trophectoderm of blastocysts. TS cells cultured in the presence of fibroblast growth factor 4 (Fgf4) are in an undifferentiated state and express undifferentiated marker genes such as Cdx2. After removing Fgf4 from the culture medium, TS cells drastically reduce the expression of undifferentiated marker genes, stop cell proliferation, and differentiate into all trophoblast cell subtypes. Read More

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http://dx.doi.org/10.1089/scd.2018.0068DOI Listing
February 2019
2 Reads

The Reparative Effects of Human Adipose-Derived Mesenchymal Stem Cells in the Chemotherapy-Damaged Thymus.

Stem Cells Dev 2019 Feb 10;28(3):186-195. Epub 2019 Jan 10.

1 Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Hematological patients who accept chemotherapy always develop secondary tumor or even die of severe infections. As an important central lymphoid organ, the thymus is frequently damaged during chemotherapy. Previous studies showed that the mesenchymal stem cells (MSCs) can promote the proliferation and repair of epithelial cells in thymus. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0142
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http://dx.doi.org/10.1089/scd.2018.0142DOI Listing
February 2019
6 Reads

Biomaterials and Gene Manipulation in Stem Cell-Based Therapies for Spinal Cord Injury.

Stem Cells Dev 2019 Feb 28;28(4):239-257. Epub 2019 Jan 28.

1 Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical University, Dalian, China.

Spinal cord injury (SCI), a prominent health issue, represents a substantial portion of the global health care burden. Stem cell-based therapies provide novel solutions for SCI treatment, yet obstacles remain in the form of low survival rate, uncontrolled differentiation, and functional recovery. The application of engineered biomaterials in stem cell therapy provides a physicochemical microenvironment that mimics the stem cell niche, facilitating self-renewal, stem cell differentiation, and tissue reorganization. Read More

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http://dx.doi.org/10.1089/scd.2018.0169DOI Listing
February 2019
3 Reads

Human Wharton's Jelly Mesenchymal Stem Cells Show Unique Gene Expression Compared with Bone Marrow Mesenchymal Stem Cells Using Single-Cell RNA-Sequencing.

Stem Cells Dev 2019 Feb 7;28(3):196-211. Epub 2019 Jan 7.

1 Department of Obstetrics and Gynaecology, National University of Singapore, Singapore, Singapore.

Human Wharton's jelly stem cells (hWJSCs) isolated from the human umbilical cord are a unique population of mesenchymal stem cells (MSCs) with significant clinical utility. Their broad differentiation potential, high rate of proliferation, ready availability from discarded cords, and prolonged maintenance of stemness properties in culture make them an attractive alternative source of MSCs with therapeutic value compared with human bone marrow MSCs (hBMMSCs). We aimed to characterize the differences in gene expression profiles between these two stem cell types using single-cell RNA sequencing (scRNA-Seq) to determine which pathways are involved in conferring hWJSCs with their unique properties. Read More

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http://dx.doi.org/10.1089/scd.2018.0132DOI Listing
February 2019
8 Reads

Equine Cord Blood Mesenchymal Stromal Cells Have Greater Differentiation and Similar Immunosuppressive Potential to Cord Tissue Mesenchymal Stromal Cells.

Stem Cells Dev 2019 Feb 14;28(3):227-237. Epub 2019 Jan 14.

Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Canada.

Mesenchymal stromal cells (MSCs) are the most common cell population studied for therapeutic use in veterinary medicine. MSCs obtained from neonatal sources such as umbilical cord tissue (CT-MSCs) or cord blood (CB-MSCs) are appealing due to the non-invasive nature of procurement and the time allowed for characterization of cells before use. However, it remains unclear as to whether CB- or CT-MSCs have equivalent progenitor and non-progenitor functions. Read More

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http://dx.doi.org/10.1089/scd.2018.0135DOI Listing
February 2019
1 Read

Characterization and Therapeutic Uses of Exosomes: A New Potential Tool in Orthopedics.

Stem Cells Dev 2019 Jan 18;28(2):141-150. Epub 2018 Dec 18.

3 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee.

In recent years, regenerative medicine has directed its interests onto the use of stem cells to heal human tissues. One specific class of cells that has been used in this field of research is mesenchymal stem cells (MSCs). Because of difficulties with the usage of whole stem cells, researchers have turned to an alternative, the secretome of the MSCs. Read More

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http://dx.doi.org/10.1089/scd.2018.0205DOI Listing
January 2019
1 Read

VE-Cadherin and ACE Co-Expression Marks Highly Proliferative Hematopoietic Stem Cells in Human Embryonic Liver.

Stem Cells Dev 2019 Feb 14;28(3):165-185. Epub 2019 Jan 14.

5 André Lwoff Institute (IFR89), Villejuif, France.

Despite advances to engineer transplantable hematopoietic stem and progenitor cells (HSPCs) for research and therapy, an in-depth characterization of the developing human hematopoietic system is still lacking. The human embryonic liver is at the crossroad of several hematopoietic sites and harbors a complex hematopoietic hierarchy, including the first actively dividing HSPCs that will further seed the definitive hematopoietic organs. However, few are known about the phenotypic and functional HSPC organization operating at these stages of development. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0154
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http://dx.doi.org/10.1089/scd.2018.0154DOI Listing
February 2019
10 Reads

Platypus Induced Pluripotent Stem Cells: The Unique Pluripotency Signature of a Monotreme.

Stem Cells Dev 2019 Feb 9;28(3):151-164. Epub 2019 Jan 9.

2 Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Australia.

The platypus (Ornithorhynchus anatinus) is an egg-laying monotreme mammal whose ancestors diverged ∼166 million years ago from the evolutionary pathway that eventually gave rise to both marsupial and eutherian mammals. Consequently, its genome is an extraordinary amalgam of both ancestral reptilian and derived mammalian features. To gain insight into the evolution of mammalian pluripotency, we have generated induced pluripotent stem cells from the platypus (piPSCs). Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0179
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February 2019
17 Reads

Extracellular Vesicles from Wharton's Jelly Mesenchymal Stem Cells Suppress CD4 Expressing T Cells Through Transforming Growth Factor Beta and Adenosine Signaling in a Canine Model.

Stem Cells Dev 2019 Feb 14;28(3):212-226. Epub 2019 Jan 14.

1 Regenerative Medicine Laboratory, Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts.

Mesenchymal stem cells (MSCs) are widely investigated as potential therapeutic agents due to their potent immunomodulatory capacity. Although specific mechanisms by which MSC acts on immune cells are emerging, many questions remain, including the potential of extracellular vesicles (EVs) to mediate biological activities. Canine MSCs are of interest for both veterinary and comparative models of disease and have been shown to suppress CD4 T cell proliferation. Read More

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http://dx.doi.org/10.1089/scd.2018.0097DOI Listing
February 2019
2 Reads
3.727 Impact Factor

Enhancing Retention of Human Bone Marrow Mesenchymal Stem Cells with Prosurvival Factors Promotes Angiogenesis in a Mouse Model of Limb Ischemia.

Stem Cells Dev 2019 Jan 18;28(2):114-119. Epub 2018 Dec 18.

1 Stem Cell Program, Department of Internal Medicine, University of California Davis Medical Center, Sacramento, California.

Mesenchymal stem/stromal cells (MSCs) offer great promise in the treatment of ischemic injuries, including stroke, heart infarction, and limb ischemia. However, poor cell survival after transplantation remains a major obstacle to achieve effective MSC therapies. To improve cell survival and retention, we transplanted human bone marrow MSCs with or without a specific prosurvival factor (PSF) cocktail consisting of IGF1, Bcl-X, a caspase inhibitor, a mitochondrial pathway inhibitor, and Matrigel into the limbs of immune deficient mice, after induction of hindlimb ischemia. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0090
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http://dx.doi.org/10.1089/scd.2018.0090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350414PMC
January 2019
17 Reads

Enhancement of Graft-Versus-Host Disease Control Efficacy by Adoptive Transfer of Type 1 Regulatory T Cells in Bone Marrow Transplant Model.

Stem Cells Dev 2019 Jan 1;28(2):129-140. Epub 2018 Dec 1.

1 Institute for Translational Research and Molecular Imaging, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Interleukin (IL)-10-producing type 1 regulatory T (Tr1) cells, which are Foxp3memory T lymphocytes, play important roles in peripheral immune tolerance. We investigated whether Tr1 cells exert immunoregulatory effects in a mouse model of acute graft-versus-host disease (GVHD). Mouse CD4 T cells were induced to differentiate in vitro into Tr1 cells using vitamin D3 and dexamethasone, and these donor-derived Tr1 cells were infused on the day of bone marrow transplantation. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0113
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January 2019
12 Reads

A Novel Human Pluripotent Stem Cell-Derived Neural Crest Model of Treacher Collins Syndrome Shows Defects in Cell Death and Migration.

Stem Cells Dev 2019 Jan 18;28(2):81-100. Epub 2018 Dec 18.

1 Anne McLaren Laboratory, Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Department of Medicine, University of Cambridge, Cambridge, United Kingdom.

The neural crest (NC) is a transient multipotent cell population present during embryonic development. The NC can give rise to multiple cell types and is involved in a number of different diseases. Therefore, the development of new strategies to model NC in vitro enables investigations into the mechanisms involved in NC development and disease. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2017.0234
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http://dx.doi.org/10.1089/scd.2017.0234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350417PMC
January 2019
14 Reads

Discovery of Natural Compounds Promoting Cardiomyocyte Differentiation.

Stem Cells Dev 2019 Jan 4;28(1):13-27. Epub 2018 Dec 4.

1 Department of Molecular Cell Biology and Sungkyunkwan University School of Medicine, Suwon, Korea.

The commitment of pluripotent stem cells to the cardiac lineage has enormous potential in regenerative medicine interventions for several cardiac diseases. Thus, it is necessary to understand and regulate this differentiation process for potential clinical application. In this study, we developed defined conditions with chemical inducers for effective cardiac lineage commitment and elucidated the mechanism for high-efficiency differentiation. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0153
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January 2019
14 Reads

Telomerase Reverse Transcriptase Expression in Mouse Endometrium During Reepithelialization and Regeneration in a Menses-Like Model.

Stem Cells Dev 2019 Jan 27;28(1):1-12. Epub 2018 Nov 27.

1 The Ritchie Centre, Hudson Institute of Medical Research, Clayton, Australia.

The regenerative capacity of the endometrium has been attributed to resident stem/progenitor cells. A number of stem/progenitor markers have been reported for human endometrial stem/progenitor cells; however, the lack of convenient markers in the mouse has made experimental investigation into endometrial regeneration difficult. We recently identified endometrial epithelial, endothelial, and immune cells, which express a reporter for the stem/progenitor marker, mouse telomerase reverse transcriptase (mTert). Read More

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http://dx.doi.org/10.1089/scd.2018.0133DOI Listing
January 2019
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Transient Cell-to-Cell Signaling Before Mitosis in Cultures of Human Bone Marrow-Derived Mesenchymal Stem/Stromal Cells.

Stem Cells Dev 2019 Jan 20;28(2):120-128. Epub 2018 Dec 20.

1 Department of Pain Medicine, Anesthesiology, Critical Care and Pain Medicine Division, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Some types of cells, if not all, that undergo signal exchanges in culture need to contact other cells for various reasons, such as cell-to-cell contact for growth inhibition. However, signal exchanges by cell-to-cell contact before proliferation have never been reported. Using time-lapse recording, we discovered the emergence of several astonishing cell-to-cell contact modes in bone marrow-derived mesenchymal stem/stromal cells (MSCs) before the cells divided. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0165
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January 2019
6 Reads

Author Accountability in Biomedical Research.

Stem Cells Dev 2018 Nov 20. Epub 2018 Nov 20.

4 Department of Laboratory Medicine, Karolinska Institutet , Stockholm, Sweden .

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https://www.liebertpub.com/doi/10.1089/scd.2018.0214
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http://dx.doi.org/10.1089/scd.2018.0214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302908PMC
November 2018
8 Reads

Human Cortical Neuron Generation Using Cell Reprogramming: A Review of Recent Advances.

Stem Cells Dev 2018 Nov 22. Epub 2018 Nov 22.

Department of Pharmacology and Clinical Pharmacology, Centre for Brain Research, School of Medical Science, Faculty of Medical and Health Sciences, University of Auckland , Auckland, New Zealand .

The study and treatment of neurological disorders have been hampered by a lack of access to live, healthy, or disease-affected human neurons. The recent advances in the field of cell reprogramming offer exciting new possibilities for disease modeling, drug development, and cell-based therapies. Since the derivation of human embryonic stem cells (hESCs) and their differentiation into neurons, cell reprogramming technologies have built on these protocols to generate mature human neurons of disease-associated phenotypes from somatic cells. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0122
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http://dx.doi.org/10.1089/scd.2018.0122DOI Listing
November 2018
17 Reads

Therapeutic Effects of Human Umbilical Cord Mesenchymal Stromal Cells in Sprague-Dawley Rats with Percutaneous Exposure to Sulfur Mustard.

Stem Cells Dev 2019 Jan 20;28(1):69-80. Epub 2018 Nov 20.

2 Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention of PLA, Beijing, China.

Sulfur mustard (SM) exposure, whose symptoms are similar to radiation exposure, can lead to acute injury. Because mesenchymal stromal cells (MSCs) have been used to experimentally and clinically treat acute radiation syndrome, in this study, MSCs were intravenously injected into rats after percutaneous SM exposure. Then, we examined sternum and spleen samples by histopathological and immunohistochemical methods to observe pathological changes. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0143
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http://dx.doi.org/10.1089/scd.2018.0143DOI Listing
January 2019
10 Reads

Stress Forces First Lineage Differentiation of Mouse Embryonic Stem Cells; Validation of a High-Throughput Screen for Toxicant Stress.

Stem Cells Dev 2019 Jan 7;28(2):101-113. Epub 2019 Jan 7.

1 CS Mott Center for Human Growth and Development, Department of Ob/Gyn, Reproductive Endocrinology and Infertility, Wayne State University School of Medicine, Detroit, Michigan.

Mouse Embryonic Stem Cells (mESCs) are unique in their self-renewal and pluripotency. Hypothetically, mESCs model gestational stress effects or stresses of in vitro fertilization/assisted reproductive technologies or drug/environmental exposures that endanger embryos. Testing mESCs stress responses should diminish and expedite in vivo embryo screening. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0157
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http://dx.doi.org/10.1089/scd.2018.0157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350416PMC
January 2019
10 Reads

Human Fetal Liver Mesenchymal Stem Cell-Derived Exosomes Impair Natural Killer Cell Function.

Stem Cells Dev 2019 Jan;28(1):44-55

1 INSERM UMR-S1197, Villejuif, France.

Mesenchymal stem cells (MSCs) are powerful immunomodulators that regulate the diverse functions of immune cells involved in allogeneic reactions, such as T cells and natural killer (NK) cells, through cell-cell contact or secreted factors. Exosomes secreted by MSCs may be involved in their regulatory functions, providing new therapeutic tools. Here, we showed that fetal liver (FL) MSC-derived exosomes inhibit proliferation, activation, and cytotoxicity of NK cells. Read More

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https://www.liebertpub.com/doi/10.1089/scd.2018.0015
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http://dx.doi.org/10.1089/scd.2018.0015DOI Listing
January 2019
7 Reads