499,141 results match your criteria Stem Cells and Cloning: Advances and Applications [Journal]


Controllable fabrication of hydroxybutyl chitosan/oxidized chondroitin sulfate hydrogels by 3D bioprinting technique for cartilage tissue engineering.

Biomed Mater 2018 Dec 17. Epub 2018 Dec 17.

Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, CHINA.

Biological regeneration of articular cartilage continues to be a challenge at present. Functional engineered implants with patient-specific sizes are difficult to achieve. The aim of this study is to fabricate a biocompatible cell-laden hydrogel with a designable structure. Read More

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December 2018

Apatinib inhibits tumor growth and angiogenesis in PNET models.

Endocr Connect 2018 Dec 1. Epub 2018 Dec 1.

Z Wang, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.

Angiogenesis has a pivotal role in the growth and metastasis of pancreatic neuroendocrine tumors (PNETs). Apatinib inhibits angiogenesis as a highly selective VEGFR2 inhibitor and has been used to treat advanced gastric cancer and malignancies in clinical settings. However, the efficacy of apatinib in PNETs remains unclear. Read More

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December 2018

Antibacterial and photocatalytic activity of hydrothermally synthesized SnO doped GO and CNT under visible light irradiation.

J Photochem Photobiol B 2018 Dec 12;191:18-25. Epub 2018 Dec 12.

Department of Civil Engineering, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea. Electronic address:

Bacterial and dye pollution are major problems with wastewater treatment. An increasing number of photocatalysts are being used in industry to kill bacterial and reduce pollution. In the present study, highly stable SnO-doped nanocomposites have been prepared successively by a hydrothermal method. Read More

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December 2018

A mouse model for intellectual disability caused by mutations in the X-linked 2'‑O‑methyltransferase Ftsj1 gene.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany.

Mutations in the X chromosomal tRNA 2'‑O‑methyltransferase FTSJ1 cause intellectual disability (ID). Although the gene is ubiquitously expressed affected individuals present no consistent clinical features beyond ID. In order to study the pathological mechanism involved in the aetiology of FTSJ1 deficiency-related cognitive impairment, we generated and characterized an Ftsj1 deficient mouse line based on the gene trapped stem cell line RRD143. Read More

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December 2018

Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology.

Acta Biomater 2018 Dec 14. Epub 2018 Dec 14.

National & Regional United Engineering Lab of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China; Center of Regenerative and Reconstructive Engineering Technology in Chongqing City, Chongqing, China. Electronic address:

Among various bone tissue engineering strategies, selective cell retention (SCR) technology has been used as a practical clinical method for bone graft manufacturing in real time. The more mesenchymal stem cells (MSCs) are retained, the better the osteoinductive microenvironment provided by the scaffold, which in turn promotes the osteogenesis of the SCR-fabricated bone grafts. Integrin receptors are crucial to cell-matrix adhesion and signal transduction. Read More

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December 2018

Various strategies to improve efficacy of stem cell transplantation in multiple sclerosis: Focus on mesenchymal stem cells and neuroprotection.

J Neuroimmunol 2018 Dec 1;328:20-34. Epub 2018 Dec 1.

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS) which predominantly affect young adults and undergo heavy socioeconomic burdens. Conventional therapeutic modalities for MS mostly downregulate aggressive immune responses and are almost insufficient for management of progressive course of the disease. Mesenchymal stem cells (MSCs), due to both immunomodulatory and neuroprotective properties have been known as practical cells for treatment of neurodegenerative diseases like MS. Read More

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December 2018

5-methylcytosine and 5-hydroxymethylcytosine in brains of patients with multiple system atrophy and patients with Parkinson's disease.

J Chem Neuroanat 2018 Dec 14. Epub 2018 Dec 14.

Department of Neurology, University of Bonn, Germany; German Center for Neurodegenerative Diseases (DZNE), University of Bonn, Germany.

Multiple system atrophy (MSA) is a sporadic neurodegenerative disorder of unknown etiology, characterized pathologically by α-synuclein aggregates preferentially found in oligodendroglial cells. DNA methylation has emerged as a mechanism of regulation of α-synuclein expression. Reduced 5-methylcytosine (5-mC) DNA methylation of α-synuclein has been found in the brains of patients with Parkinson's disease (PD). Read More

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December 2018

KIT as a therapeutic target for non-oncological diseases.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

Aix Marseille Université, INSERM, INRA, C2VN, Marseille, France; Clinique des Bronches, Allergies et Sommeil, Hôpital Nord, AP-, HM, Marseille, France.. Electronic address:

KIT is a receptor tyrosine kinase that after binding to its ligand stem cell factor activates signaling cascades linked to biological processes such as proliferation, differentiation, migration and cell survival. Based on studies performed on SCF and/or KIT mutant animals that presented anemia, sterility, and/or pigmentation disorders, KIT signaling was mainly considered to be involved in the regulation of hematopoiesis, gametogenesis, and melanogenesis. More recently, novel animal models and ameliorated cellular and molecular techniques have led to the discovery of a widen repertoire of tissue compartments and functions that are being modulated by KIT. Read More

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December 2018
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Fine structure of the midgut epithelium of Thulinius ruffoi (Tardigrada, Eutardigrada, Parachela) in relation to oogenesis and simplex stage.

Arthropod Struct Dev 2018 Dec 14. Epub 2018 Dec 14.

University of Silesia in Katowice, Department of Animal Histology and Embryology, Bankowa 9, 40-007 Katowice, Poland. Electronic address:

Thulinius ruffoi is a small freshwater tardigrade that lives in both non-polluted and polluted freshwater environments. As a result of tardigradan body miniaturization, the digestive system is reduced and simplified. It consists of a short fore- and hindgut, and the midgut in the shape of a short tube is lined with a simple epithelium. Read More

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December 2018

Induced Pluripotent Stem Cells: A New Strategy to Model Human Cancer.

Int J Biochem Cell Biol 2018 Dec 14. Epub 2018 Dec 14.

Laboratory for Cancer Biology, Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, India. Electronic address:

Induced pluripotent stem cells are derived from adult somatic cells by ectopic expression of stem cell factors OCT4, SOX2, MYC and KLF4. These cells have characteristic features similar to embryonic stem cells. Although there exists in vitro and in vivo models of cancer, recapitulating the earliest events in the pathogenesis remain challenging. Read More

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December 2018
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Revised version - Correction unmarked effective elimination of biofilm formed with waterborne pathogens using copper nanoparticles.

Microb Pathog 2018 Dec 14. Epub 2018 Dec 14.

Department of Chemistry, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, 613401, India. Electronic address:

In this paper, the self assembling properties of taurolipids were used to prepare stable copper nanoparticles (CuNPs), and demonstrated the ability of CuNPs to eradicate the biofilms formed by waterborne pathogens. The synthesized CuNPs display wine red color and exhibited surface plasmon resonance with a maximum at 590 nm. Transmission electron microscopy showed that the CuNPs are well-dispersed with spherical morphology and the size range between 5 and 12 nm. Read More

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December 2018

Development of a Graft Inoculation Method and a Real-Time RT-PCR Assay for Monitoring Tomato chlorosis virus Infection in Tomato.

J Virol Methods 2018 Dec 14. Epub 2018 Dec 14.

Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Agricultural Biotechnology, 32260, Isparta, Turkey.

A graft inoculation method coupled with RT-qPCR was developed for monitoring ToCV infection in tomato plants. Ten seed-grown tomato seedlings were graft inoculated with phloem tissue-containing stem segments from a ToCV-infected tomato plants. Another group of tomato seedling were grafted with similar stem segments from a healthy tomato plant as mock inoculated control. Read More

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December 2018

Corneal regeneration by conditioned medium of human uterine cervical stem cells is mediated by TIMP-1 and TIMP-2.

Exp Eye Res 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, Faculty of Science, University of A Coruña, Spain. Electronic address:

The aim off the present study was to evaluate the effect and the mechanism of action of the conditioned medium from human uterine cervical stem cells (CM-hUCESC) on corneal wound healing in a rabbit dry eye model. To do this, dry eye and corneal epithelial injuries were induced in rabbits by topical administration of atropine sulfate and NaOH. Hematoxylin-Eosin (H&E) and Ki-67 immunostaining were carried out to evaluate corneal damage and cell proliferation, and real-time PCR was used to evaluate proinflammatory cytokines in the cornea. Read More

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December 2018

Pluripotency markers in tissue and cultivated cells in vitro of different regions of human amniotic epithelium.

Exp Cell Res 2018 Dec 14. Epub 2018 Dec 14.

Instituto Nacional de Perinatología, Departamento de Fisiología y Desarrollo Celular, Ciudad de México, México. Electronic address:

Studies have described the presence of pluripotent markers in vivo and in vitro in human amnion. However, the amnion can be divided into reflected, placental and umbilical regions that are anatomically and functionally heterogeneous. Here, we evaluated the expression of pluripotency markers in tissue and cultivated cells in vitro of different regions of human amnion. Read More

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December 2018

Lung cancer stem cells: origin, features, maintenance mechanisms and therapeutic targeting.

Biochem Pharmacol 2018 Dec 14. Epub 2018 Dec 14.

Department of Medical Oncology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. Electronic address:

Lung cancer remains the leading cause of cancer-related deaths despite recent breakthroughs in immunotherapy. The widely embraced cancer stem cell (CSC) theory has also been applied for lung cancer, postulating that an often small proportion of tumor cells with stem cell properties are responsible for tumor growth, therapeutic resistance and metastasis. The identification of these CSCs and underlying molecular maintenance mechanisms is considered to be absolutely necessary for developing therapies for their riddance, hence achieving remission. Read More

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December 2018

Fabrication of scaffold-free tubular cardiac constructs using a Bio-3D printer.

PLoS One 2018 17;13(12):e0209162. Epub 2018 Dec 17.

Department of Regenerative Medicine and Biomedical Engineering, Faculty of Medicine, Saga University, Saga, Japan.

A major challenge in cardiac tissue engineering is the host's immune response to artificial materials. To overcome this problem, we established a scaffold-free system for assembling cell constructs using an automated Bio-3D printer. This printer has previously been used to fabricate other three-dimensional (3D) constructs, including liver, blood vessels, and cartilage. Read More

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December 2018

Small-molecule induction of Aβ-42 peptide production in human cerebral organoids to model Alzheimer's disease associated phenotypes.

PLoS One 2018 17;13(12):e0209150. Epub 2018 Dec 17.

Service d'Etude des Prions et Infections Atypiques (SEPIA), Institut François Jacob, CEA, Université Paris-Saclay, Fontenay-aux-Roses, France.

Human mini-brains (MB) are cerebral organoids that recapitulate in part the complexity of the human brain in a unique three-dimensional in vitro model, yielding discrete brain regions reminiscent of the cerebral cortex. Specific proteins linked to neurodegenerative disorders are physiologically expressed in MBs, such as APP-derived amyloids (Aβ), whose physiological and pathological roles and interactions with other proteins are not well established in humans. Here, we demonstrate that neuroectodermal organoids can be used to study the Aβ accumulation implicated in Alzheimer's disease (AD). Read More

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December 2018
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A graph-based evidence synthesis approach to detecting outbreak clusters: An application to dog rabies.

PLoS Comput Biol 2018 Dec 17;14(12):e1006554. Epub 2018 Dec 17.

MRC Centre for Outbreak Analysis and Modelling, Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, United Kingdom.

Early assessment of infectious disease outbreaks is key to implementing timely and effective control measures. In particular, rapidly recognising whether infected individuals stem from a single outbreak sustained by local transmission, or from repeated introductions, is crucial to adopt effective interventions. In this study, we introduce a new framework for combining several data streams, e. Read More

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December 2018

The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea.

PLoS Genet 2018 Dec 17;14(12):e1007828. Epub 2018 Dec 17.

Max Planck Institute for Molecular Biomedicine, Münster, Germany.

In multicellular organisms, cell type diversity and fate depend on specific sets of transcript isoforms generated by post-transcriptional RNA processing. Here, we used Schmidtea mediterranea, a flatworm with extraordinary regenerative abilities and a large pool of adult stem cells, as an in vivo model to study the role of Uridyl-rich small nuclear RNAs (UsnRNAs), which participate in multiple RNA processing reactions including splicing, in stem cell regulation. We characterized the planarian UsnRNA repertoire, identified stem cell-enriched variants and obtained strong evidence for an increased rate of UsnRNA 3'-processing in stem cells compared to their differentiated counterparts. Read More

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December 2018
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Stem cell therapies for Alzheimer's disease: is it time?

Curr Opin Psychiatry 2018 Dec 14. Epub 2018 Dec 14.

Department of Psychiatry, Yeouido St. Mary's Hospital.

Purpose Of Review: Stem cell therapy has the potential to modify the disease of Alzheimer's disease. This article aims to describe the mechanisms of action, preclinical animal studies, human clinical trials, and challenges for the future direction of stem cell therapy for Alzheimer's disease.

Recent Findings: Stem cells of diverse origins (embryonic, placental or umbilical cord blood, and induced pluripotent stem cells) and cell types (neural and mesenchymal stem cells) are widely studied in both animals and humans. Read More

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December 2018

Immature electrophysiological properties of human-induced pluripotent stem cell-derived neurons transplanted into the mouse cortex for 7 weeks.

Neuroreport 2018 Dec 15. Epub 2018 Dec 15.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo.

The transplantation of human-induced pluripotent stem cell (hiPSC)-derived cells has emerged as a potential clinical approach for the treatment of brain diseases. Recent studies with animal disease models have shown that hiPSC-derived neurons transplanted into the brain, especially the nigrostriatal area, could restore degenerated brain functions. Further works are required to test whether hiPSC-derived neurons can also gain functional properties for other cortical areas. Read More

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December 2018

Differences of Immune Reconstitution of Dendritic Cells in Pediatric GvHD Patients After Allogenic Stem Cell Transplantation.

J Pediatr Hematol Oncol 2018 Nov 30. Epub 2018 Nov 30.

Department of Pediatric Hematology and Oncology, University Hospital Würzburg, Germany.

Background: Hematopoietic stem cell transplantation (HSCT) is a life-saving procedure for children with a variety of (non) malignant conditions. GvHD is a severe complication with high morbidity and mortality. The pathogenesis remains unclear. Read More

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November 2018

Evaluation of mature miR398 family, expression analysis and the post transcriptional regulation evidence in gamma-irradiated and nitrogen stressed Medicago sativa seedlings.

Int J Radiat Biol 2018 Dec 17:1-36. Epub 2018 Dec 17.

a Department of Biotechnology, School of Life Sciences , Pondicherry University , Kalapet , Puducherry - 605014 , India.

Background: The miRNA398 (microRNA398) post transcriptionally regulates the superoxide dismutase (Cu/Zn-SOD).

Materials And Methods: The miRNA398 level were quantitated in gamma irradiated 20Gy and 200Gy and nitrogen stressed seedlings by stem-loop RT-PCR (Real Time- Polymerase chain reaction). The positional preference of nucleotide for miR398 families and new targets was done. Read More

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December 2018
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Reversibility of castration resistance status after Radium-223 dichloride treatment: Clinical evidence and Review of the literature.

Int J Radiat Biol 2018 Dec 17:1-29. Epub 2018 Dec 17.

a Department of Radiological Sciences, Oncology and Anatomical Pathology , Sapienza University of Rome , Viale Regina Elena 324 , 00161 , Rome , Italy.

Purpose: In the history of prostate cancer, some of patients progresses to castration resistant prostate cancer (CRPC) stage and, although new drugs and treatment protocols have been introduced, CRPC presents poor prognosis. This review focused on biological mechanisms, underlying CRPC described in scientific literature in order to explain the reversion of resistance to castration. We present the case of a 73 years-old man, affected by bone metastatic CRPC, early treated with Radium-223 with a complete response. Read More

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December 2018

Kinetics of the topochemical transformation of (PbSe)m(TiSe2)n(SnSe2)m(TiSe2)n to (Pb0.5Sn0.5Se)m(TiSe2)n.

J Am Chem Soc 2018 Dec 17. Epub 2018 Dec 17.

Solid state reaction kinetics on atomic length scales have not been heavily investigated due to the long times, high reaction temperatures, and small reaction volumes at interfaces in solid state reactions. All of these conditions present significant analytical challenges in following reaction pathways. Herein we use in-situ and ex-situ x-ray diffraction, in-situ x-ray reflectivity, high angle annular dark field scanning transmission electron microscopy, and energy dispersive x-ray spectroscopy to investigate the mechanistic pathways for the formation of a layered (Pb0. Read More

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December 2018

A Computational Simulation of Cyclic Stretch of an Individual Stem Cell Using a Nonlinear Model.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Division of Biomedical Engineering, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.

Many experiments have shown that mechanical stimuli like cyclic strains might be helpful in stem cell differentiation. To maximize such differentiations efficiency, it is imperative to detect the cellular mechanical responses to these stimuli. The purpose of this research was to show that a newly presented hyper-viscoelastic model could correctly predict the level of stresses required to obtain a different response from a single mesenchymal stem cell cultured in a fibrin hydrogel block under a 10 percent cyclic strain at a frequency of 1 Hertz, employing finite element method. Read More

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December 2018
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Antimicrobial effects of the ginsenoside Rh2 on monospecies and multispecies cariogenic biofilms.

J Appl Microbiol 2018 Dec 17. Epub 2018 Dec 17.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan, 430079, China.

Aims: To investigate the effects of the ginsenoside Rh2 on monospecies and multispecies cariogenic biofilms and explore the mechanism of the antibiofilm effect of Rh2 in vitro.

Methods And Results: Streptococcus mutans, Streptococcus sobrinus, and Streptococcus sanguinis were chosen to form the monospecies or multispecies biofilms. Crystal violet staining and laser scanning confocal microscopy were used to observe the effect of Rh2 on biofilms in vitro. Read More

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December 2018

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

J Cell Physiol 2018 Dec 17. Epub 2018 Dec 17.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Our previous study showed that knocking down integrin α5 (ITGA5) expression by using a lentiviral vector in human dental pulp stem cells (DPSCs) led to weakening proliferation and migration capacity while enhanced odontogenic differentiation. To seek for possible clinical application, we investigated the effect of the ITGA5 priming synthetic cyclic peptide (SCP; GA-CRRETAWAC-GA) on proliferation, migration, and the odontogenic differentiation of DPSCs. Remarkably, the involved mechanism was explored by isobaric tag for relative and absolute quantitation proteomic technique, and the in vivo effect of ITGA5 was investigated by nude mice subcutaneous transplantation of cell and hydroxyapatite/β-tricalcium phosphate complex. Read More

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December 2018

Comparing Hydroxyapatite with Osteogenic Medium for the Osteogenic Differentiation of Mesenchymal Stem Cells on PHBV Nanofibrous Scaffolds.

J Biomater Sci Polym Ed 2018 Dec 17:1-21. Epub 2018 Dec 17.

a Emergency Center of the Affiliated Hospital of Xuzhou Medical University, Institute of Emergency Rescue Medicine, Key Laboratory of New Drugs and Clinical Application , Xuzhou Medical University , Xuzhou , 221000 , China.

Having advantageous biocompatibility and osteoconductive properties known to enhance the osteogenic differentiation of mesenchymal stem cells (MSCs), hydroxyapatite (HA) is a commonly used material for bone tissue engineering. What remains unclear, however, is whether HA holds a similar potential for stimulating the osteogenic differentiation of MSCs to that of a more frequently used osteogenic-inducing medium (OIM). To that end, we used PHBV electrospun nanofibrous scaffolds to directly compare the osteogenic capacities of HA with OIM over MSCs. Read More

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December 2018

CD73 as a potential opportunity for cancer immunotherapy.

Expert Opin Ther Targets 2018 Dec 17. Epub 2018 Dec 17.

i Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran .

Introduction: Cancer cells apply various mechanisms to induce and enhance immune escape. The complex network of immune-response modulating factors in the tumor microenvironment is a reason for the difficulties encountered when attempting to treat many cancers. Adenosine is a potent immune-modulating factor that can be generated through the degradation of ATP by cooperative action of NTPDase1 (CD39) and ecto-5'-nucleotidase (CD73) molecules. Read More

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December 2018

The value of the post-thaw CD34+ count with and without DMSO removal in the setting of autologous stem cell transplantation.

Transfusion 2018 Dec 17. Epub 2018 Dec 17.

Division of Hematology, University Hospital Basel, Switzerland.

Background: CD34+ cell count correlates with engraftment potency after autologous stem cell transplantation. Assessment of CD34+ mainly occurs after apheresis and before cryopreservation with dimethyl sulfoxide (DMSO). The influence of postthaw CD34+ cell numbers over time to engraftment is not well studied, and determination of postthaw CD34+ cell counts is challenging for a variety of reasons. Read More

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December 2018

Logical stimuli-triggered delivery of small molecules from hydrogel biomaterials.

Biomater Sci 2018 Dec 17. Epub 2018 Dec 17.

Department of Chemical Engineering, University of Washington, 3781 Okanogan Lane NE, Seattle, WA 98195, USA. and Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA 98105, USA and Institute of Stem Cell & Regenerative Medicine, University of Washington, 850 Republican St., Seattle, WA 98109, USA and Molecular Engineering & Sciences Institute, University of Washington, 3946 W Stevens Way NE, Seattle, WA 98105, USA.

Stimuli-responsive biomaterials are useful platforms for environmentally triggered drug delivery. By varying the molecular architecture of orthogonal stimuli-labile linkages between small molecules and non-degradable materials, we demonstrate the Boolean logic-based release of model therapeutics from gels. Programmable responses are demonstrated for materials sensitive to input combinations involving enzymes, chemical reductants, and light via YES, OR, and AND logic gates. Read More

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December 2018

MEG3 affects the progression and chemoresistance of T-cell lymphoblastic lymphoma by suppressing epithelial-mesenchymal transition via the PI3K/mTOR pathway.

J Cell Biochem 2018 Dec 16. Epub 2018 Dec 16.

Department of Hematology, Hematopoietic Stem Cell Transplantation/Cell Immunotherapy Center, Cheng Du Military General Hospital of PLA, Cheng Du, China.

Long noncoding RNAs (lncRNA) are emerging as integral functional and regulatory components in the development of different diseases including cancer. Maternally expressed gene 3 (MEG3), is a lncRNA, that has a depressed expression in multiple tumor types, including T-cell lymphoblastic lymphoma (T-LBL). However, the molecular mechanisms that regulate the tumorigenic functions of MEG3 in T-LBL remain largely unknown. Read More

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December 2018

Packaging functionally-important plasma proteins into the α-granules of human induced pluripotent stem cell-derived megakaryocytes.

J Tissue Eng Regen Med 2018 Dec 17. Epub 2018 Dec 17.

Blood Center of Wisconsin, Blood Research Institute, Milwaukee, WI.

The contents of platelet α-granules arrive via a number of pathways; some are synthesized by megakaryocytes (MKs), e.g., von Willebrand Factor (VWF), while others are endocytosed from plasma, e. Read More

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December 2018

Lower gamma globulin level before conditioning is a risk factor for cytomegalovirus antigenemia after pediatric allogeneic stem cell transplantation.

Pediatr Blood Cancer 2018 Dec 17:e27586. Epub 2018 Dec 17.

Department of Hematology/Oncology for Children and Adolescents, Sapporo Hokuyu Hospital, Hokkaido, Japan.

Background: Despite the development of early detection methods and new antiviral drugs, cytomegalovirus (CMV) infection remains a persistent and sometimes severe complication of stem cell transplantation (SCT). CMV antigenemia has become widely used for early detection of CMV infection after SCT.

Procedure: We retrospectively analyzed risk factors for CMV antigenemia in pediatric patients following allogeneic SCT. Read More

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December 2018

Promising immunotherapy: Highlighting cytokine-induced killer cells.

J Cell Biochem 2018 Dec 16. Epub 2018 Dec 16.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

For many years, cancer therapy has appeared to be a challenging issue for researchers and physicians. By the introduction of novel methods in immunotherapy, the prospect of cancer therapy even more explained than before. Cytokine-induced killer (CIK) cell-based immunotherapy demonstrated to have potentiality in improving clinical outcomes and relieving major side effects of standard treatment options. Read More

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December 2018
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Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin.

J Cell Biochem 2018 Dec 16. Epub 2018 Dec 16.

Combat Trauma and Burn Injury Research, United States Army Institute of Surgical Research, Fort Sam Houston, Texas.

This study demonstrates that adipose-derived stem cells from debrided skin (dsASCs) of burn patients can be isolated in sufficient quantities and differentiated into cytokeratin-expressing cells by treating them with all-trans retinoic acid (ATRA) and the peroxisome proliferator-activated receptor-α (PPARα) specific activator fenofibrate. Differentiation of dsASCs with ATRA and a combination of growth factors induced expression of simple epithelial markers (KRT7, KRT8, KRT18, and KRT19), along with low levels of stratified epithelial markers (KRT5, KRT10, KRT13, and KRT14). We have optimized a condition to induce dsASCs differentiation to epithelial cells by treatment with ATRA and fenofibrate alone. Read More

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December 2018
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Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium.

J Cell Biochem 2018 Dec 16. Epub 2018 Dec 16.

Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran.

We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM-MSC) individually and/or in combination on the stereological parameters and the expression of basic fibroblast growth factor (bFGF), hypoxia-inducible factor (HIF-1α), and stromal cell-derived factor-1α (SDF-1α) in a wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) in diabetic rats. CM was provided by culturing hBM-MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups, harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM + PBMT. Read More

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December 2018
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Risk of solid subsequent malignant neoplasms after childhood Hodgkin lymphoma-identification of high-risk populations to guide surveillance: A report from the Late Effects Study Group.

Cancer 2018 Dec 17. Epub 2018 Dec 17.

Institute for Cancer Outcomes and Survivorship, University of Alabama at Birmingham, Birmingham, Alabama.

Background: Survivors of Hodgkin lymphoma (HL) in childhood have an increased risk of subsequent malignant neoplasms (SMNs). Herein, the authors extended the follow-up of a previously reported Late Effects Study Group cohort and identified patients at highest risk for SMNs to create evidence for risk-based screening recommendations.

Methods: The standardized incidence ratio was calculated using rates from the Surveillance, Epidemiology, and End Results program as a reference. Read More

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December 2018

Chemical composition, antioxidant and antimicrobial potential of essential oils from different parts of Daphne mucronata Royle.

Chem Cent J 2018 Dec 17;12(1):135. Epub 2018 Dec 17.

Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

This research work was executed to determine chemical composition, anti-oxidant and anti-microbial potential of the essential oils extracted from the leaves and stem of Daphne mucronata Royle. From leaves and stem oils fifty-one different constituents were identified through GC/MS examination. The antioxidant potential evaluated through DPPH free radical scavenging activity and %-inhibition of peroxidation in linoleic acid system. Read More

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December 2018

Prevalence of growth and endocrine disorders in Malaysian children with transfusion-dependent thalassaemia.

Singapore Med J 2018 Dec 17. Epub 2018 Dec 17.

Department of Paediatrics, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia.

Introduction: Endocrine dysfunction due to iron overload secondary to frequent blood transfusions is a common complication in children with transfusion-dependent thalassaemia (TDT). We ascertained the prevalence of endocrine dysfunction in children with TDT seen in a hospital setting in Malaysia.

Methods: We reviewed all patients with TDT who had ≥ 8 blood transfusions per year. Read More

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December 2018

Phytotoxic Potential of and Its Major Compound Linarin as a Possible Natural Herbicide.

ACS Omega 2018 Nov 2;3(11):14779-14787. Epub 2018 Nov 2.

Centro de Investigaciones Químicas, IICBA, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico.

Four compounds, the flavone linarin (), the triterpene lupenone (), the tocopherol (vitamin E, ), and the new natural alkaloid 1,2,3,4-tetrahydro-1,1-dimethyl-6,7-isoquinolindiol (affineine, ), were the major natural products isolated from (syn. , Rutaceae). Compound is highly abundant in this plant and was isolated as a white precipitate obtained from the acetone and methanol extracts. Read More

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November 2018

Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration.

ACS Omega 2018 Nov 1;3(11):14620-14633. Epub 2018 Nov 1.

Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai625021, India.

Presently, tissue engineering approaches have been focused toward finding new potential scaffolds with osteoconductivity on bone-disease-affected cells. This work focused on the cisplatin (CDDP)-loaded graphene oxide (GO)/hydroxyapatite (HAP)/chitosan (CS) composite for enhancing the growth of osteoblast cells and prevent the development of osteosarcoma cells. The prepared composites were characterized for the confirmation of composite formation using Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray diffraction techniques. Read More

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November 2018
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Experimental data in support of characterization of the CePO dispersion into transparent PMMA/PU IPNs by the sequential route.

Data Brief 2018 Dec 15;21:2350-2359. Epub 2018 Nov 15.

Department of Materials, Loughborough University, Loughborough LE11 3TU, United Kingdom.

This article is focused on the complementary data referring to the article "Dispersion of upconverting nanostructures of CePO using rod and semi-spherical morphologies into transparent PMMA/PU IPNs by the sequential route". It contains the XPS data of CePO photographs and DSC thermograms of transparent PMMA/PU IPNs as well as with CePO dispersed in different wt.%, Confocal laser scanning micrographs, transmission electron microscopy (TEM), optical images of surface, and visual inspection (photographs) before and after aging of hybrid materials. Read More

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December 2018

The orphan nuclear receptor EAR-2 (NR2F6) inhibits hematopoietic cell differentiation and induces myeloid dysplasia in vivo.

Biomark Res 2018 7;6:36. Epub 2018 Dec 7.

3Department of Medical Biophysics, University of Toronto, Sunnybrook Research Institute, Toronto, ON M4N 3M5 Canada.

Background: In patients with myelodysplastic syndrome (MDS), bone marrow cells have an increased predisposition to apoptosis, yet MDS cells outcompete normal bone marrow (BM)-- suggesting that factors regulating growth potential may be important in MDS. We previously identified v-Erb A related-2 (EAR-2, NR2F6) as a gene involved in control of growth ability.

Methods: Bone marrow obtained from C57BL/6 mice was transfected with a retrovirus containing EAR-2-IRES-GFP. Read More

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December 2018

Alarming levels of antimicrobial resistance among sepsis patients admitted to ICU in a tertiary care hospital in India - a case control retrospective study.

Antimicrob Resist Infect Control 2018 7;7:150. Epub 2018 Dec 7.

1Dr. B. R. Ambedkar Centre for Biomedical Research (ACBR), University of Delhi (North Campus), Delhi, 110007 India.

Background: Hospital acquired infections (HAI) are principal threats to the patients of intensive care units. An increase in the antimicrobial resistance (AMR) observed in gram negative bacteria is a great challenge to deal with. HAI and AMR lead to prolonged hospitalization and additional doses of anti-microbial treatment affecting patient's fitness and finances. Read More

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December 2018

Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):202-207

Department of Medical Biotechnology, Semnan University of Medical Sciences, Semnan, Iran.

Background: Bone Morphogenetic Protein-2 (BMP-2) is a cysteine rich growth factor expressed in homodimeric form and has a pivotal role in osteochondral development and fracture healing. Recent studies have benefited more from recombinant BMP-2 in osteochondral tissue engineering. Cost-effective and easy production at large scale makes () the first choice for recombinant protein expression programs. Read More

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December 2018

Prostaglandin E2 produced by myeloid-derived suppressive cells induces cancer stem cells in uterine cervical cancer.

Oncotarget 2018 Nov 20;9(91):36317-36330. Epub 2018 Nov 20.

Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Osaka, Japan.

Myeloid-derived suppressor cells (MDSCs) enhance tumor progression by suppressing tumor-specific T cell responses, stimulating tumor angiogenesis, or promoting tumor cell metastasis. However, the biology of MDSCs have not been fully investigated. In the current study, we investigated the role of MDSCs in inducing cancer stem-like cells and explored a clinically feasible approach for targeting MDSCs-mediated cancer stem-like cells induction. Read More

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November 2018

The estrogen receptor variants β2 and β5 induce stem cell characteristics and chemotherapy resistance in prostate cancer through activation of hypoxic signaling.

Oncotarget 2018 Nov 20;9(91):36273-36288. Epub 2018 Nov 20.

University of Houston, Department of Biology and Biochemistry, Center for Nuclear, Receptors and Cell Signaling, Science and Engineering Research Center, Houston, Texas, USA.

Chemotherapy resistant prostate cancer is a major clinical problem. When the prostate cancer has become androgen deprivation resistant, one of the few treatment regimens left is chemotherapy. There is a strong connection between a cancer's stem cell like characteristics and drug resistance. Read More

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November 2018

Adoptive Cell Transfer: Is it a Promising Immunotherapy for Colorectal Cancer?

Theranostics 2018 10;8(20):5784-5800. Epub 2018 Nov 10.

Department of Pharmacology, College of Medicine, The Pennsylvania State University, Hershey, PA, USA.

The last decade has witnessed significant advances in the adoptive cell transfer (ACT) technique, which has been appreciated as one of the most promising treatments for patients with cancer. Utilization of ACT can enhance the function of the immune system or improve the specificity and persistence of transferred cells. Various immune cells including T lymphocytes, natural killer cells, dendritic cells, and even stem cells can be used in the ACT despite their different functional mechanisms. Read More

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November 2018