513,733 results match your criteria Stem Cells and Cloning: Advances and Applications [Journal]


Corneal Endothelial Decompensation after Ocular Chemical Burn: Description of a New Finding.

Klin Monbl Augenheilkd 2019 Apr 18;236(4):371-376. Epub 2019 Apr 18.

Augenklinik, Universitatsspital Basel, Basel, Switzerland.

Background: Ocular chemical burn is an ophthalmological emergency. Therefore, chemical injuries should be promptly addressed in order to initiate the appropriate treatment as soon as possible and optimize the visual prognosis. We present a retrospective study of ten cases with ocular chemical burn including one with superglue and one with a liquid plaster material injury and describe their clinical course. Read More

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http://dx.doi.org/10.1055/a-0857-6538DOI Listing

Screening for mutations in selected miRNA genes in hypogonadotropic hypogonadism patients.

Endocr Connect 2019 May;8(5):506-509

Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland.

In approximately half of congenital hypogonadotropic hypogonadism (cHH) patients, the genetic cause remains unidentified. Since the lack of certain miRNAs in animal models has led to cHH, we sequenced human miRNAs predicted to regulate cHH-related genes (MIR7-3, MIR141, MIR429 and MIR200A-C) in 24 cHH patients with Sanger sequencing. A heterozygous variant in MIR200A (rs202051309; general population frequency of 0. Read More

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http://dx.doi.org/10.1530/EC-19-0080DOI Listing

Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation.

Stem Cell Res 2019 Apr 10;37:101434. Epub 2019 Apr 10.

Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA. Electronic address:

Patient-derived induced pluripotent stem cells (iPSCs) have become a promising resource for exploring genetics of complex diseases, discovering new drugs, and advancing regenerative medicine. Increasingly, laboratories are creating their own banks of iPSCs derived from diverse donors. However, there are not yet standardized guidelines for qualifying these cell lines, i. Read More

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http://dx.doi.org/10.1016/j.scr.2019.101434DOI Listing

Effects of perfluorooctanoic acid on stem Leydig cell functions in the rat.

Environ Pollut 2019 Apr 9;250:206-215. Epub 2019 Apr 9.

Department of Pediatrics, Peking University First Hospital, No.1 Xi'an Men Street, West District, Beijing, 100034, China; Department of Pediatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China. Electronic address:

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic (PFOS) are two perfluorinated chemical products widely existing in the environment. Evidence suggested that PFOA might relate to male reproductive dysfunction in rats and humans. PFOA exposure inhibited the function of Leydig cells. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.03.120DOI Listing

A novel neutral and thermophilic endoxylanase from Streptomyces ipomoeae efficiently produced xylobiose from agricultural and forestry residues.

Bioresour Technol 2019 Mar 27;285:121293. Epub 2019 Mar 27.

College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, PR China; Guangxi Key Laboratory of Biorefinery, Nanning 530003, Guangxi, PR China. Electronic address:

Endoxylanases capable of producing high ratios of xylobiose from agricultural and forestry residues in neutral and high temperature conditions are attractive for the prebiotic and alternative sweetener industries. In this study, a putative glycosyl hydrolase gene from Streptomyces ipomoeae was cloned and expressed in Escherichia coli. The recombinant enzyme, named as SipoEnXyn10A, hydrolyzed beechwood xylan in endo-action mode releasing xylobiose as its main end product. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.03.132DOI Listing

Superparamagnetic iron oxide nanoparticles of curcumin enhance gemcitabine therapeutic response in pancreatic cancer.

Biomaterials 2019 Apr 8;208:83-97. Epub 2019 Apr 8.

Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, USA. Electronic address:

Pancreatic cancer is a complex disease accounting for fibrotic tumors and an aggressive phenotype. Gemcitabine (GEM) is used as a standard therapy, which develops chemoresistance leading to poor patient outcome. We have recently developed a superparamagnetic iron oxide nanoparticle (SPION) formulation of curcumin (SP-CUR), which is a nontoxic, bioactive anti-inflammatory/anti-cancer agent for its enhanced delivery in tumors. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.005DOI Listing

Strontium-incorporated mineralized PLLA nanofibrous membranes for promoting bone defect repair.

Colloids Surf B Biointerfaces 2019 Apr 6;179:363-373. Epub 2019 Apr 6.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address:

Mineralized scaffolds, which are fabricated by electrodeposition, mimic the chemistry of natural bone and have attracted a great amount of attention due to their rapid and simple production. In this study, mineralized electrospun polylactic acid (PLLA) nanofibrous membranes containing different amounts of strontium (Sr) were fabricated by an electrodeposition method for potential use in bone regeneration applications. In vitro assays, including cell proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) grown on these membranes and in vivo cranial bone defect repair assays, were carried out. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.011DOI Listing

Developing biocompatible silver nanoparticles using epigallocatechin gallate for dental use.

Arch Oral Biol 2019 Mar 28;102:106-112. Epub 2019 Mar 28.

Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address:

Objective: To develop silver nanoparticles (AgNPs) using epigallocatechin gallate (EGCG) and evaluate its biocompatibility and inhibition effect on Streptococcus mutans biofilm growth.

Design: AgNPs were synthesized using EGCG as a reducing agent. Cytotoxicity was assessed using half-maximal inhibitory concentration (IC) against human gingival fibroblast (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Read More

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http://dx.doi.org/10.1016/j.archoralbio.2019.03.022DOI Listing

Mechanisms underlying a critical period of respiratory development in the rat.

Respir Physiol Neurobiol 2019 Apr 15. Epub 2019 Apr 15.

Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive, SE, Albuquerque, New Mexico 87108, USA.

Twenty-five years ago, Filiano and Kinney (1994) proposed that a critical period of postnatal development constitutes one of the three risk factors for sudden infant death syndrome (SIDS). The underlying mechanism was poorly understood. In the last 17 years, much has been uncovered on this period in the rat. Read More

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http://dx.doi.org/10.1016/j.resp.2019.04.006DOI Listing

Egg white coated alginate nanoparticles with electron sprayer for potential anticancer application.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, PR China; Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address:

To development biomimetic strategy for enhanced cancer therapy, the paclitaxel loaded egg white/sodium alginate nanoparticles were prepared by electronic spray method. Their appearance and particle size were observed by transmission electron microscope and particle size analyzer. The drug release behavior of nanoparticles was determined by high performance liquid chromatography (HPLC). Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.045DOI Listing

Intravenous Infusion of Mesenchymal Stem Cells Improves Impaired Cognitive Function in a Cerebral Small Vessel Disease Model.

Neuroscience 2019 Apr 15. Epub 2019 Apr 15.

Department of Neural Regenerative Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan; Department of Neurology, Yale University School of Medicine, New Haven, CT, 06510, USA; Center for Neuroscience and Regeneration Research, VA Connecticut Healthcare System, West Haven, CT, 06516, USA.

Cerebral small vessel disease (CSVD) is not only a cause of vascular dementia (VD) but also a contributing factor to Alzheimer's disease (AD). The essential pathological feature of CSVD is the disruption of blood-brain barrier (BBB). Dysfunction of BBB due to degeneration of both endothelial cells and pericytes in capillaries leads to neuronal damage and progressive brain atrophy. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.04.018DOI Listing

New anti-HBV norbisabolane sesquiterpenes from Phyllantus acidus.

Fitoterapia 2019 Apr 15. Epub 2019 Apr 15.

Center for Pharmaceutical Sciences, Faculty of Life Science and Technology, Kunming University of Science and Technology, Chenggong Campus, Kunming 650500, People's Republic of China. Electronic address:

The norbisabolane-type sesquiterpenoids bearing a spiroketal functionality have been found in Phyllanthus spp. and showed anti-HBV activities. As part of an ongoing effort to search for promising anti-HBV sesquiterpenes from Phyllanthus plants, we report four new norbisabolane-type sesquiterpenoids, phyacidusin A (1), phyacidusin B (2), phllanthacidoid A1 (3) and phllanthacidoid N1 (4), from stem of P. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.04.006DOI Listing

Sex-Specific Neurogenic Deficits and Neurocognitive Disorders in Middle-Aged HIV-1 Tg26 Transgenic Mice.

Brain Behav Immun 2019 Apr 15. Epub 2019 Apr 15.

Center for Metabolic Disease Research, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, United States; Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, United States. Electronic address:

Varying degrees of cognitive deficits affect over half of all HIV-1 infected patients. Because of antiretroviral treatment (ART) regimens, the HIV-1 patient population is increasing in age. Very few epidemiological studies have focused on sex-specific differences in HIV-1-associated neurocognitive disorders (HAND). Read More

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http://dx.doi.org/10.1016/j.bbi.2019.04.029DOI Listing

Leaves and stems of Capparis erythrocarpos, more sustainable than roots, show antiarthritic effects.

J Ethnopharmacol 2019 Apr 15:111890. Epub 2019 Apr 15.

Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Ethnopharmacological Relevance: Capparis erythrocarpos is a medicinal plant used widely in many parts of Africa for the management of pain and inflammatory conditions such as rheumatoid arthritis. Its wide range of use, popularity and high value, make C. erythrocarpos containing products a target for economically driven adulteration. Read More

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http://dx.doi.org/10.1016/j.jep.2019.111890DOI Listing

Evaluation of the schistosomicidal, antioxidant and anti-inflammatory activities of the ethyl acetate fraction from Ozoroa pulcherrima Schweinf. Roots on Schistosoma mansoni-induced liver pathology in mice and its phytochemical characterization.

J Ethnopharmacol 2019 Apr 15:111883. Epub 2019 Apr 15.

Laboratory of Biology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; Centre for Schistosomiasis and Parasitology, P.O. Box 7244, Yaoundé, Cameroon. Electronic address:

Ethnopharmacological Relevance: Ozoroa pulcherrima Schweinf. (syn.: Heeria pulcherrrima Schweinf. Read More

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http://dx.doi.org/10.1016/j.jep.2019.111883DOI Listing

High-fat Diet Accelerates Carcinogenesis in a Mouse Model of Barrett's Esophagus via IL8 and Alterations to the Gut Microbiome.

Gastroenterology 2019 Apr 15. Epub 2019 Apr 15.

Department of Internal Medicine, Technical University of Munich, Germany. Electronic address:

Background & Aims: Barrett's esophagus (BE) is a precursor to esophageal adenocarcinoma (EAC). Progression from BE to cancer is associated with obesity, possibly due to increased abdominal pressure and gastroesophageal reflux disease, although this pathogenic mechanism has not been proven. We investigated whether environmental or dietary factors associated with obesity contribute to progression of BE to EAC in mice. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.04.013DOI Listing
April 2019
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Epigenetic regulation of immediate-early gene Nr4a2/Nurr1 in the medial habenula during reinstatement of cocaine-associated behavior.

Neuropharmacology 2019 Apr 15. Epub 2019 Apr 15.

Department of Neurobiology and Behavior, School of Biological Sciences University of California, Irvine, USA; UC Irvine Center for Addiction Neuroscience, School of Biological Sciences, University of California, Irvine, USA; Center for the Neurobiology of Learning and Memory, School of Biological Sciences, University of California, Irvine, USA. Electronic address:

Propensity to relapse following long-periods of abstinence is a key feature of substance use disorder. Drugs of abuse, such as cocaine, cause long-term changes in the neural circuitry regulating reward, motivation, and memory processes through dysregulation of various molecular mechanisms, including epigenetic regulation of activity-dependent gene expression. Underlying drug-induced changes to neural circuit function are the molecular mechanisms regulating activity-dependent gene expression. Read More

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http://dx.doi.org/10.1016/j.neuropharm.2019.04.016DOI Listing

Stress responses in stromal cells and tumor homeostasis.

Pharmacol Ther 2019 Apr 15. Epub 2019 Apr 15.

Department of Radiation Oncology, Weill Cornell Medical College, New York, NY, USA; Sandra and Edward Meyer Cancer Center, New York, NY, USA; Department of Dermatology, Yale School of Medicine, New Haven, CT, USA; Université Paris Descartes/Paris V, Paris, France. Electronic address:

In most (if not all) solid tumors, malignant cells are outnumbered by their non-malignant counterparts, including immune, endothelial and stromal cells. However, while the mechanisms whereby cancer cells adapt to microenvironmental perturbations have been studied in great detail, relatively little is known on stress responses in non-malignant compartments of the tumor microenvironment. Here, we discuss the mechanisms whereby cancer-associated fibroblasts and other cellular components of the tumor stroma react to stress in the context of an intimate crosstalk with malignant, endothelial and immune cells, and how such crosstalk influences disease progression and response to treatment. Read More

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http://dx.doi.org/10.1016/j.pharmthera.2019.04.004DOI Listing

Mesenchymal Stem Cells: from Regeneration to Cancer.

Pharmacol Ther 2019 Apr 15. Epub 2019 Apr 15.

The Jackson Laboratory, Bar Harbor, ME 04609, USA. Electronic address:

Mesenchymal stem cells (MSCs) are multipotent tissue stem cells that differentiate into a number of mesodermal tissue types, including osteoblasts, adipocytes, chondrocytes and myofibroblasts. MSCs were originally identified in the bone marrow (BM) of humans and other mammals, but recent studies have shown that they are multilineage progenitors in various adult organs and tissues. MSCs that localize at perivascular sites function to rapidly respond to external stimuli and coordinate with the vascular and immune systems to accomplish the wound healing process. Read More

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http://dx.doi.org/10.1016/j.pharmthera.2019.04.005DOI Listing

Disruption of RNA Metabolism in Neurological Diseases and Emerging Therapeutic Interventions.

Neuron 2019 Apr;102(2):294-320

Department of Neurology, The Sean M. Healey and AMG Center for ALS at Mass General, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA; Broad Institute of Harvard University and MIT, Cambridge, MA 02142, USA. Electronic address:

RNA binding proteins are critical to the maintenance of the transcriptome via controlled regulation of RNA processing and transport. Alterations of these proteins impact multiple steps of the RNA life cycle resulting in various molecular phenotypes such as aberrant RNA splicing, transport, and stability. Disruption of RNA binding proteins and widespread RNA processing defects are increasingly recognized as critical determinants of neurological diseases. Read More

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http://dx.doi.org/10.1016/j.neuron.2019.03.014DOI Listing

Sister, Sister: Ependymal Cells and Adult Neural Stem Cells Are Separated at Birth by Geminin Family Members.

Neuron 2019 Apr;102(2):278-279

Department of Pathology, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA. Electronic address:

The adult subventricular zone (SVZ) stem cell niche is comprised of multi-ciliated ependymal cells that line the brain ventricular system and adult stem cells. Papers in Neuron (Ortiz-Álvarez et al., 2019) and Cell Reports (Redmond et al. Read More

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http://dx.doi.org/10.1016/j.neuron.2019.02.040DOI Listing

Golden Palladium Zinc Ordered Intermetallics as Oxygen Reduction Electrocatalysts.

ACS Nano 2019 Apr 18. Epub 2019 Apr 18.

Exploring Pt-free electrocatalysts with high activity and long durability for the oxygen reduction reaction (ORR) has been long pursued by the renewable energy material community. In this work, we have designed an ordered intermetallics PdZn/C (O-PdZn) with a several atomic-layer Pd shell, which achieved a three-fold enhancement in ORR mass activities (MA) in alkaline media, relative to Pd/C and Pt/C. Further Au incorporation in O-PdZn/C (Au-O-PdZn/C) yielded a catalyst with superior durability with less than 10% loss in MA after 30,000 potential cycles. Read More

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http://dx.doi.org/10.1021/acsnano.9b01961DOI Listing

Therapeutic Window for Phosphodiesterase 6-Related Retinitis Pigmentosa.

JAMA Ophthalmol 2019 Apr 18. Epub 2019 Apr 18.

Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Departments of Ophthalmology and Pathology and Cell Biology, Columbia Stem Cell Initiative, Institute of Human Nutrition, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, New York.

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http://dx.doi.org/10.1001/jamaophthalmol.2018.6381DOI Listing

Frequency of hematologic and solid malignancies in the family history of 50 patients with acute myeloid leukemia - a single center analysis.

PLoS One 2019 18;14(4):e0215453. Epub 2019 Apr 18.

Department of Medicine III, University Hospital Munich, Ludwig-Maximilians-University Munich-Campus Großhadern, Munich, Germany.

Background And Objective: The revised World Health Organization classification of 2016 for myeloid neoplasms and acute leukemia added a section of myeloid neoplasms with germline predisposition. The main objective of our study was to evaluate the frequency of hematologic and solid malignancies in the family history of patients with acute myeloid leukemia (AML) by using a systemic pedigree interview. The family history was taken of 50 patients between 24 and 80 years. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215453PLOS

Purging of malignant cell contamination prior to spermatogonia stem cell autotransplantation to preserve fertility: progress & prospects.

Curr Opin Endocrinol Diabetes Obes 2019 Apr 16. Epub 2019 Apr 16.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

Purpose Of Review: This systematic review evaluates the state of the art in terms of strategies used to detect and remove contaminated malignant cells from testicular biopsy prior to spermatogonia stem cells (SSCs) autotransplantation to restore fertility.

Recent Findings: Several trials have been done in past two decades to determine the reliable methods of detecting and purging cancer cells prior to SSCs autotransplantation.

Summary: The success in treating childhood cancer has dramatically increased over the past few decades. Read More

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http://dx.doi.org/10.1097/MED.0000000000000481DOI Listing

Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B.

JCI Insight 2019 Apr 18;5. Epub 2019 Apr 18.

The bone marrow microenvironment (BMME) contributes to the regulation of hematopoietic stem cell (HSC) function, though its role in age-associated lineage skewing is poorly understood. Here we show that dysfunction of aged marrow macrophages (Mφs) directs HSC platelet-bias. Mφs from the marrow of aged mice and humans exhibited an activated phenotype, with increased expression of inflammatory signals. Read More

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http://dx.doi.org/10.1172/jci.insight.124213DOI Listing

Identification, Pathogenicity, and Spore Trapping of Associated with Twig and Shoot Dieback in California.

Plant Dis 2019 Apr 17:PDIS08181425RE. Epub 2019 Apr 17.

5 Department of Plant Pathology, University of California, Davis, CA 95616.

Corda, 1831 species are well-documented pathogens of citrus that are associated with leaf and fruit anthracnose diseases. However, their role in twig and shoot dieback diseases of citrus has recently become more prominent. Recent surveys of orchards in the Central Valley of California have revealed . Read More

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http://dx.doi.org/10.1094/PDIS-08-18-1425-REDOI Listing

Favorable Bioactivity of the SDHI Fungicide Benzovindiflupyr Against Mycelial Growth, Sclerotial Production, and Myceliogenic and Carpogenic Germination of Sclerotia.

Plant Dis 2019 Apr 17:PDIS05180729RE. Epub 2019 Apr 17.

1 Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, P. R. China.

, which can cause Sclerotinia stem rot, is a prevalent plant pathogen. This study aims to evaluate the application potential of benzovindiflupyr, a new generation of succinate dehydrogenase inhibitor (SDHI), against . In our study, 181 isolates collected from different crops (including eggplant [ = 34], cucumber [ = 27], tomato [ = 29], pepper [ = 35], pumpkin [ = 32], and kidney bean [ = 25]) in China were used to establish baseline sensitivity to benzovindiflupyr. Read More

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http://dx.doi.org/10.1094/PDIS-05-18-0729-REDOI Listing

Intravesical electromotive administration of botulinum toxin type A in improving the bladder and bowel functions: Evidence for novel mechanism of action.

J Spinal Cord Med 2019 Apr 18:1-7. Epub 2019 Apr 18.

b Department of pathology , Shariati Hospital, Tehran University of Medical Sciences , Tehran , Iran.

Objective: To examine the hypothesis that what is the concomitant mechanism of action botulinum toxin type A (BoNTA) administration by intravesical electromotive into the bladder resulting in bladder function improvement. We also tried to confirm the possibility of retrograde trans-axonal transportation of toxin.

Design: Animal study. Read More

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http://dx.doi.org/10.1080/10790268.2019.1603490DOI Listing

Transplantation of Hearts Donated After Circulatory-Determined Death.

Circ Heart Fail 2019 Apr;12(4):e005991

Scottish National Advanced Heart Failure Service, Golden Jubilee National Hospital, Glasgow, United Kingdom (M.C.P.).

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http://dx.doi.org/10.1161/CIRCHEARTFAILURE.119.005991DOI Listing

Deficiency of X-ray repair cross-complementing group 1 in primordial germ cells contributes to male infertility.

FASEB J 2019 Apr 18:fj201801962RR. Epub 2019 Apr 18.

State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China.

X-ray repair cross-complementing group 1 (), a key DNA repair gene, plays a vital role in maintaining genomic stability and is highly expressed in the early stages of spermatogenesis, but the exact functions remain elusive. Here we generated primordial germ cell-specific knockout (c) mice to elucidate the effects of on spermatogenesis. We demonstrated that deficiency results in infertility in male mice due to impaired spermatogenesis. Read More

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http://dx.doi.org/10.1096/fj.201801962RRDOI Listing

Screening Therapeutic Agents Specific to Breast Cancer Stem Cells Using a Microfluidic Single-Cell Clone-Forming Inhibition Assay.

Small 2019 Apr 18:e1901001. Epub 2019 Apr 18.

Department of Laboratory Medicine, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.

Screens of cancer stem cells (CSCs)-specific agents present significant challenges to conventional cell assays due to the difficulty in preparing CSCs ready for drug testing. To overcome this limitation, developed is a microfluidic single-cell assay for screening breast cancer stem cell-specific agents. This assay takes advantage of the single-cell clone-forming capability of CSCs, which can be specifically inhibited by CSC-targeting agents. Read More

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http://dx.doi.org/10.1002/smll.201901001DOI Listing

Vessel Tortuosity Cutoff Values Using the Modified ROPtool May Predict Need for Treatment in Retinopathy of Prematurity.

Ophthalmic Surg Lasers Imaging Retina 2019 Apr;50(4):215-220

Background And Objective: To quantify vessel tortuosity among infants with retinopathy of prematurity (ROP).

Patients And Methods: This was a retrospective study including 61 RetCam images from 33 infants. The laser treatment (LT) group included 17 infants who underwent laser for ROP. Read More

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http://dx.doi.org/10.3928/23258160-20190401-03DOI Listing

[Donor-Specific Antibodies in Allogeneic Hematopoietic Stem Cell Transplantation-Review].

Authors:
Li Wang

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):629-632

Department of Hematology, The 923th Hospital of the People's Liberation Army of China, Nanning 530021, Guangxi Zhuang Autonomous Region, China E-mail: wl923yy@ 163.com.

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is widely used in the treatment of hematological diseases, HLA incompatibility is displayed in most cases. Some patients have prestored donor-specific antibodies (DSA) or new emerging antibodies after transplantation. Donor-specific antibodies may cause graft failure and poor graft function ,and affect transplant outcome. Read More

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http://dx.doi.org/10.19746/j.cnki.issn1009-2137.2019.02.052DOI Listing

[Establishment of STO Cell Lines Expressing Green Fluorescent Protein and Mouse Leukemia Inhibitory Factor].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):606-612

Department of Pathophysiology, Bengbu Medical College, Bengbu 233030, Anhui Province, China E-mail:

Objective: To establish the STO cell lines expressing green fluorescent protein (GFP) and mouse leukemia inhibitory factor (LIF) , and try to culture the mouse embryonic stem cells (mESCs) by using the established STO-GFP-mLIF cells as the feeder layer.

Methods: The lentiviral particles containing GFP and mLIF and puromycin-resistance gene were constructed and transduced into STO cell lines. The cell lines stably expressing GFP and mLIF genes were screened out. Read More

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http://dx.doi.org/10.19746/j.cnki.issn1009-2137.2019.02.048DOI Listing

[Role of Rictor in Hematopoietic Stem Cells during Fetal Liver Hematopoiesis].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):600-605

State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.E-mail:

Objective: To investigate the effect of Rictor on the hematopoiesis of fetal liver by specific knock-out of Rictor in hematopoietic cells of Vav-Cre mice.

Methods: E12.5 0. Read More

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http://dx.doi.org/10.19746/j.cnki.issn1009-2137.2019.02.047DOI Listing

[Effect of Microvascular Endothelial Cells on the Proliferation of Bone Marrow Hematopoietic Stem Cells under Different Culture Conditions].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):594-599

Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan Province, China,E-mail:

Objective: To investigate the effect of microvascular endothelial cells (MEC) on the proliferation of hematopoictic stem cells (HSC) under different culture conditions in vitro.

Methods: The MEC from lung tissue of C57BL/6 mice and the HSC from bone marrow of GFP mice were used for non-contact co-culture, 2 D contact co-culture, at same time the single MEC and single HSC culture were seted up and were used as control group. The cell counting and CCK-8 method were used to detect and compare the proliferation levels of suspension cells in different groups on day 1, 3, 5 and 7. Read More

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http://dx.doi.org/10.19746/j.cnki.issn1009-2137.2019.02.046DOI Listing

[Screening and Verification of Antioxidant Small Molecular Compounds for Expansion of Human Hematopoietic Stem Cells Ex Vitro].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):585-593

Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China

Objective: To screen the antioxidant small molecular compounds with optimal efficiency of expansing the human hematopoietic stem cells (hHSC) In vitro based on antioxidant small molecular compound database of LKT laboratory, and to verify the effects of these compounds on the biological functions of hHSC.

Methods: The umbilial cord blood CD34 cells were enriched by using the MACS beads; the absolute number and percentage of CD34 cells and CD34 CD49f cells were detected by high throughput flow cytometry after culture of hHSC with compounds in vitro for 1 week, the SR1 (1 μmol/L) was used as positive control, the candidate compounds were screened out; then 4 compounds were selected for follow-up experiments by comprehensive evaluation of concentration, safety and expansion efficacy, the optimal used concentrations of selected compounds were determined through the concentration gradient analysis, and CFC short-term colony-forming cell test was performed by using the determined concentration so as to verify the effect of compounds on the self-renewal, multilineage differentiation.

Results: Out of 85 antioxidant small molecular compounds, 4 compounds (C2968, D3331, B1753 and B3358) with obvious expansion efficacy for CD34 cells and CD34 CD49f cells were screened out by high throughput flow cytometry; their optimal concentrations of 4 compounds were 0. Read More

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http://dx.doi.org/10.19746/j.cnki.issn1009-2137.2019.02.045DOI Listing

[Association between IL-10 Gene -592(C→A) (rs1800872) SNP and GVHD after Allogeneic Hematopoietic Stem Cell Transplantation in Children].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):573-579

Hematology Oncology Center of Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing Key Laboratory of Pediatric Hematology Oncology; National Key Discipline of Pediatrics (Capital Medical University); Key Laboratory of Major Diseases in Children, Ministry of Education; Beijing 100045, ChinaE-mail:

Objective: To study the association between IL-10 gene-592(C→A) (rs1800872) single nucleotide polymorphism (SNP) and the graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in children.

Methods: Ninety-seven childhood patients and seventy-one donors in the Hematology Oncology Center of Beijing Children's Hospital from Jan 2011 to Jul 2017 were enrolled in this study. The genomic DNA was extracted from peripheral blood cells and the SNP genotype was analyzed using TaqMan SNP genotyping assay. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.043DOI Listing

[Effects of a Traditinal Chinese Medicine Compound Regimen on Differentiation and Proliferation of Acute Myeloid Leukemia Cell Lines].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):403-408

Laboratory for Stem Cell and Regenerative Medicine,Affiliated Hospital of Weifang Medical College, Weifang 261042, Shandong Province,

Objective: To study the effect of traditional chinese medicine (TCM) compound on myeloid leukemia cells and to explore its anti-leukemic mechanism.

Methods: Myeloid leukemia cell lines were cultured in vitro and treated with TCM compound. The proliferation of the leukemia cells was measured by CCK8 method. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.015DOI Listing

[Clinical Efficacy of Modified BU/CY as Conditioning Regimen Combined with Autologous Peripheral Blood Hematopoietic Stem Cell Transplantation in Young Acute Myeloid Leukemia Patients with Low or Intermediate Risk].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):360-364

Second Clinical College, Shanxi Metical University; Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China,E-mail:

Objective: To investigate the safety and efficacy of autologous peripheral blood hematopoietic stem cell transplantation (auto-PBHSCT) using modified BU/CY conditioning regimen for young AML patients of low and middle risk in the first complete remission (CR1).

Methods: Ten young AML patients of low and middle risk who did not want to accept allogeneic hematopoietic stem cell transplantation(allo-HSCT)and underwent auto-PBHSCT in CR1 during May 2013 to December 2016 were retrospectively analyzed. From 3 months after auto-PBHSCT, the maintenance therapy with interleukin-2 (IL-2) or IL-2 combined with histamine dihydrochloride was performed for these patients in the next 18 months. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.008DOI Listing
April 2019
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[Clinical Characteristics of Patients with Ph Mixed Phenotype Acute Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):354-359

Blood Diseases Hospital, Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300020

Objective: To investigate the clinical biological characteristics and prognosis of the patients with mixed phenotype acute leukemia with t(9;22)(q34;q11.2) and/or BCRABL1 (Ph MPAL).

Methods: The morphological, immunological, cytogenetic, and molecular features of 33 in patients with Ph MPAL were retrospectively analyzed in our center from June 2002 to June 2016 according to the scoring proposal of European Group for the Classification of Acute Leukemia(EGIL )1998 and WHO 2008 criteria. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.007DOI Listing

[Bioinformatics Analysis and Verification of Aging-Related Genes of Bone Marrow Mesenchymal Stem Cells in Patients with Acute Myeloid Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):311-317

State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China,E-mail:

Objective: To screen and verify the differentially expressed genes related with aging of bone marrow mesenchymal stem cells (BM-MSCs) in acute myeloid leukemia (AML) patients by bioinformatics, so as to provide new molecular markers for the research and clinical treatment of AML.

Methods: The gene expression profiling chip related with BM-MSCs in AML patients in our hospital and the gene chip GSE84881 selected from NCBI database GEO were used for data analysis and exploration. The DAVID analysis software was used to perform gene ontology (GO) enrichment analysis and KEGG pathway enrichment analysis. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.001DOI Listing

An outreach program with hands-on, physiology-based exercises generates questions about STEM career expectations.

Adv Physiol Educ 2019 Jun;43(2):175-179

Department of Cellular and Integrative Physiology, University of Nebraska Medical Center , Omaha, Nebraska.

Scientific advocacy and outreach programs are encouraged to increase public understanding of scientific knowledge and generate interest in science, technology, engineering, and mathematics (STEM) careers. However, evaluation of these events' effectiveness is difficult and somewhat rare. This study's purpose was to better understand how effective an established physiology-based outreach program was in generating interest in STEM careers, while simultaneously providing information that can be used to increase the effectiveness of future events. Read More

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http://dx.doi.org/10.1152/advan.00013.2019DOI Listing

Beyond the single course: teaching research throughout the curriculum.

Adv Physiol Educ 2019 Jun;43(2):168-171

Department of Chemistry and Physical Science, Dakota Wesleyan University, Mitchell, South Dakota.

Our STEM majors arrive on campus as rule followers-"Tell me what to do, and I'll do it," is their refrain. To break this mindset, we redesigned our suite of laboratory courses to scaffold research throughout the program. Every laboratory incorporates both discipline-specific and research-specific skills. Read More

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http://dx.doi.org/10.1152/advan.00215.2018DOI Listing

CRISPR-Cas9-Mediated Correction of the G189R-PAX2 Mutation in Induced Pluripotent Stem Cells from a Patient with Focal Segmental Glomerulosclerosis.

CRISPR J 2019 Apr;2:108-120

1 Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy; University of Milan, Milan, Italy.

Focal segmental glomerulosclerosis (FSGS) is defined by focal (involving few glomeruli) and segmental sclerosis of the glomerular tuft that manifests with nephrotic syndrome. Mutations in genes involved in the maintenance of structure and function of podocytes have been found in a minority of these patients. A family with adult-onset autosomal dominant FSGS was recently found to carry a new germline missense heterozygous mutation (p. Read More

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http://dx.doi.org/10.1089/crispr.2018.0048DOI Listing

Differentiating Human Induced Pluripotent Stem Cells (iPSCs) Into Lung Epithelial Cells.

Curr Protoc Stem Cell Biol 2019 Apr 18:e86. Epub 2019 Apr 18.

Eyestem Research, Centre for Cellular and Molecular Platforms (CCAMP), National Centre for Biological Sciences (NCBS) Campus, Bengaluru, India.

Human induced pluripotent stem cells (hiPSCs) not only offer great opportunities for the study of human development but also have tremendous potential for future clinical cell-based therapies. The protocol outlined here is used to differentiate hiPSCs into lung epithelial cell types through a process that faithfully recapitulates the stepwise events observed in vivo. From pluripotency, cells are differentiated to a definitive endoderm fate, followed by progression into anteriorized foregut endoderm that has the ability to give rise to both proximal and distal epithelial cells. Read More

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http://dx.doi.org/10.1002/cpsc.86DOI Listing

Reconsidering osteoconduction in the era of additive manufacturing.

Authors:
Franz E Weber

Tissue Eng Part B Rev 2019 Apr 18. Epub 2019 Apr 18.

Zentrum fur Zahnmedizin der Universitat Zurich, 30992, MKG/Oral Biotechnology&Bioengineering , Plattenstrasse 11 , Zurich, Switzerland , 8032 ;

Bone regeneration procedures in clinics and bone tissue-engineering stand on three pillars: osteoconduction, osteoinduction, and stem cells. In the last two decades, the focus in this field has been on osteoinduction, realized by the use of bone morphogenetic proteins (BMPs) and the application of mesenchymal stem cells to treat bone defects. Osteoconduction, however, was reduced to a surface phenomenon, since the supposedly ideal pore size of osteoconductive scaffolds was identified in the 1990s, and with 0. Read More

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https://www.liebertpub.com/doi/10.1089/ten.TEB.2019.0047
Publisher Site
http://dx.doi.org/10.1089/ten.TEB.2019.0047DOI Listing
April 2019
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A prospective non-interventional study on the impact of transfusion burden and related iron toxicity on outcome in myelodysplastic syndromes undergoing allogeneic hematopoietic cell transplantation.

Leuk Lymphoma 2019 Apr 18:1-10. Epub 2019 Apr 18.

u University Hospital Eppendorf , Hamburg , Germany.

Most myelodysplastic syndromes (MDS)-patients receive multiple red blood cell transfusions (RBCT). Transfusions may cause iron-related toxicity and mortality, influencing outcome after allogeneic HSCT. This prospective non-interventional study evaluated 222 MDS and CMML patients undergoing HSCT. Read More

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http://dx.doi.org/10.1080/10428194.2019.1594215DOI Listing

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting.

Cell Transplant 2019 Apr 18:963689719844260. Epub 2019 Apr 18.

2 W.M. Keck Center for Collaborative Neuroscience, Rutgers, the State University of New Jersey, Piscataway, New Jersey, USA.

Multilineage-differentiating stress-enduring (Muse) cells are a population of pluripotent stage-specific embryonic antigen 3 (SSEA3)+ mesenchymal stem cells first described by Mari Dezawa in 2010. Although some investigators have reported SSEA3+ mesenchymal cells in umbilical cord tissues, none have quantitatively compared SSEA3+ cells isolated from Wharton's jelly (WJ) and the cord lining (CL) of human umbilical cords (HUCs). We separated WJ and the CL from HUCs, cultured mesenchymal stromal cells (MSCs) isolated from these two tissues with collagenase, and quantified the percentage of SSEA3+ cells over three passages. Read More

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http://dx.doi.org/10.1177/0963689719844260DOI Listing
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