68,774 results match your criteria Staphylococcal Infections


Inhibition of the ATP synthase sensitizes Staphylococcus aureus towards human antimicrobial peptides.

Sci Rep 2020 Jul 9;10(1):11391. Epub 2020 Jul 9.

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, 1870, Frederiksberg C, Denmark.

Antimicrobial peptides (AMPs) are an important part of the human innate immune system for protection against bacterial infections, however the AMPs display varying degrees of activity against Staphylococcus aureus. Previously, we showed that inactivation of the ATP synthase sensitizes S. aureus towards the AMP antibiotic class of polymyxins. Read More

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http://dx.doi.org/10.1038/s41598-020-68146-4DOI Listing

Resident macrophages acquire innate immune memory in staphylococcal skin infection.

Elife 2020 Jul 8;9. Epub 2020 Jul 8.

Institute for Immunodeficiency, Center for Chronic Immunodeficiency, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

is a common colonizer of healthy skin and mucous membranes. At the same time, is the most frequent cause of skin and soft tissue infections. Dermal macrophages (Mφ) are critical for the coordinated defense against invading yet they have a limited life span with replacement by bone marrow derived monocytes. Read More

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http://dx.doi.org/10.7554/eLife.55602DOI Listing

The Molecular Complex between Staphylococcal Adhesin SpsD and Fibronectin Sustains Mechanical Forces in the Nanonewton Range.

mBio 2020 Jul 7;11(4). Epub 2020 Jul 7.

Louvain Institute of Biomolecular Science and Technology, UCLouvain, Louvain-la-Neuve, Belgium

The bacterial pathogen is involved in canine otitis externa and pyoderma as well as in surgical wound and urinary tract infections. Invasion of canine epithelial cells is promoted by fibronectin (Fn)-binding proteins SpsD and SpsL through molecular interactions that are currently unknown. By means of single-molecule experiments, we discover that both adhesins have distinct molecular mechanisms for binding to Fn. Read More

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http://dx.doi.org/10.1128/mBio.00371-20DOI Listing

Genetic Characterization of Methicillin-Resistant Isolates from Human Bloodstream Infections: Detection of MLS Resistance.

Antibiotics (Basel) 2020 Jul 3;9(7). Epub 2020 Jul 3.

Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.

In this study we aimed to characterize antimicrobial resistance in methicillin-resistant (MRSA) isolated from bloodstream infections as well as the associated genetic lineages of the isolates. Sixteen MRSA isolates were recovered from bacteremia samples from inpatients between 2016 and 2019. The antimicrobial susceptibility of these isolates was tested by the Kirby-Bauer disk diffusion method against 14 antimicrobial agents. Read More

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http://dx.doi.org/10.3390/antibiotics9070375DOI Listing

Eradicating Infecting Bacteria while Maintaining Tissue Integration on Photothermal Nanoparticle-coated Titanium Surfaces.

ACS Appl Mater Interfaces 2020 Jul 7. Epub 2020 Jul 7.

Photothermal nanoparticles locally release heat, when NIR-irradiated. Clinical application initially involved tumor treatment, but currently extends towards bacterial infection-control. Application towards much smaller, micrometer-sized bacterial infections however, bears the risk of collateral damage by heat dissipating into tissue surrounding an infection site. Read More

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http://dx.doi.org/10.1021/acsami.0c08592DOI Listing

Staphylococcal enterotoxin M induced inflammation and impairment of bovine mammary epithelial cells.

J Dairy Sci 2020 Jul 1. Epub 2020 Jul 1.

Key Laboratory of Qinghai-Tibetan Plateau, Animal Genetic Resource Reservation and Utilization of Ministry of Education, College of Life Science and Technology, Southwest Minzu University, Chengdu, China, 610041.

Staphylococcus aureus is one of the major etiological pathogens of bovine mastitis. Its invasion into mammary epithelial cells has been proven to be a key event in the pathogenesis of mastitis. However, the specific pathogenic factors have not been clearly identified. Read More

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http://dx.doi.org/10.3168/jds.2019-17444DOI Listing

New acridine derivatives as promising agents against methicillin-resistant staphylococci - From tests to in silico analysis.

Comput Biol Chem 2020 Jun 25;88:107321. Epub 2020 Jun 25.

Department of Pharmaceutical Chemistry, Drug Analyses and Radiopharmacy, Medical University, ul. Muszyńskiego 1, 90-151 Lodz, Poland. Electronic address:

The present study describes the antibacterial activity of several new derivatives of tacrine or cyclopentaquinoline bound to either 6-hydrazinenicotinic acid or 4-fluorobenzoic acid through an aliphatic chain against methicillin-resistant staphylococcal strains. All derivatives showed antibacterial activity against all tested methicillin-resistant staphylococci. Of these, compounds 6, 18, 23 and 24 exhibited the highest activity, ranging from 4. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2020.107321DOI Listing

Tofacitinib treatment aggravates Staphylococcus aureus septic arthritis, but attenuates sepsis and enterotoxin induced shock in mice.

Sci Rep 2020 Jul 2;10(1):10891. Epub 2020 Jul 2.

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy at University of Gothenburg, Guldhedsgatan 10A, 413 46, Göteborg, Sweden.

Tofacitinib, a janus kinase inhibitor, is a novel immunosuppressive drug for treatment of rheumatoid arthritis (RA). Septic arthritis (SA) and sepsis caused by Staphylococcus aureus (S. aureus), for which RA patients are at risk, are infections with high mortality. Read More

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http://dx.doi.org/10.1038/s41598-020-67928-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331611PMC

Integra dermal regeneration template as the nidus of staphylococcal toxic shock syndrome: A case report.

JPRAS Open 2020 Sep 29;25:24-29. Epub 2020 May 29.

Professor, Chief of Plastic Surgery Division, Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

The literature describes numerous successful applications using dermal regeneration templates such as Integra. Despite widespread use for burns, trauma, and chronic wounds among others, Integra has associated complications which need continued investigation. Large multi-center studies designed to investigate its safety have shown that infections are the most common complications. Read More

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http://dx.doi.org/10.1016/j.jpra.2020.05.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321779PMC
September 2020

Using minimum inhibitory concentration values of common topical antibiotics to investigate emerging antibiotic resistance: A retrospective study of 134 dogs and 20 horses with ulcerative keratitis.

Vet Ophthalmol 2020 Jul 1. Epub 2020 Jul 1.

College of Optometry, The Ohio State University, Columbus, OH, USA.

Objectives: To identify the minimum inhibitory concentration (MIC) distribution for commonly used topical antibiotics from isolates of dogs and horses with ulcerative bacterial keratitis, and to investigate changes in MIC values over time and following treatment with topical fluoroquinolones.

Animals Studied: One hundred thirty-four client-owned dogs and 20 client-owned horses with bacterial ulcerative keratitis.

Procedure: Minimum inhibitory concentration values for 14 topical antibiotics were reported for canine and equine cases of bacterial ulcerative keratitis between 2013 and 2018. Read More

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http://dx.doi.org/10.1111/vop.12801DOI Listing

Maternal-Fetal Perinatal Transmission of Staphylococcal Infections: A Report of Two Neonates.

Case Rep Pediatr 2020 16;2020:8886049. Epub 2020 Jun 16.

Department of Pediatrics, Base Hospital, Maha Oya, Sri Lanka.

Staphylococcal infection in terms of fetus is rare and is associated with either maternal staphylococcal sepsis or colonization that leads to vertical transmission. Antenatal invasive procedures are a recognized risk factor. Most reported newborns with fetal-onset staphylococcal infections have had a worse outcome. Read More

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http://dx.doi.org/10.1155/2020/8886049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315269PMC

induces cell-surface expression of immune stimulatory NKG2D ligands on human monocytes.

J Biol Chem 2020 Jun 30. Epub 2020 Jun 30.

Experimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Experimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

is among the leading causes of bacterial infections worldwide. The pathogenicity and establishment of infections are tightly linked to its ability to modulate host immunity. Persistent infections are often associated with mutant staphylococcal strains that have decreased susceptibility to antibiotics; however, little is known about how these mutations influence bacterial interaction with the host immune system. Read More

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http://dx.doi.org/10.1074/jbc.RA120.012673DOI Listing

Staphylococcus pseudintermedius 5'-nucleotidase suppresses canine phagocytic activity.

Vet Microbiol 2020 Jul 16;246:108720. Epub 2020 May 16.

Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA. Electronic address:

Staphylococcus pseudintermedius is a major opportunistic bacterial pathogen and the leading cause of pyoderma in dogs. In canines it is also often associated with infections of the urinary system and wounds and occasionally infects people. Widespread antimicrobial resistance has made the development of alternative treatments a high priority. Read More

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http://dx.doi.org/10.1016/j.vetmic.2020.108720DOI Listing

Community-acquired pneumonia caused by methicillin-resistant Staphylococcus aureus in a Chinese adult: A case report.

Medicine (Baltimore) 2020 Jun;99(26):e20914

Department of Respiratory Medicine.

Rationale: Methicillin-resistant Staphylococcus aureus (MRSA) has been established as an important cause of severe community-acquired pneumonia (CAP) with very high mortality. Panton-Valentine leukocidin (PVL) producing MRSA has been reported to be associated with necrotizing pneumonia and worse outcome. The incidence of community-acquired MRSA (CA-MRSA) pneumonia is very low, as only a few CA-MRSA pneumonia cases were reported in the last few years. Read More

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http://dx.doi.org/10.1097/MD.0000000000020914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329007PMC

Deep surgical site infection after posterior instrumented fusion for rheumatoid upper cervical subluxation treated with antibiotic-loaded bone cement: Three case reports.

Medicine (Baltimore) 2020 Jun;99(26):e20892

Department of Orthopaedic Surgery, Saitama Medical Center, Saitama Medical University, Kawagoe, Saitama, Japan.

Introduction: Patients with rheumatoid arthritis (RA) tend to be immunosuppressed due to RA itself and the therapeutic drugs administered. The management of surgical site infection (SSI) following upper cervical spinal instrumented fusion in RA patients is challenging; however, literature on the treatment for such conditions is scarce. We report 3 consecutive patients with RA, who developed deep SSI following upper cervical posterior fusion and were treated using antibiotic-loaded bone cement (ALBC). Read More

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http://dx.doi.org/10.1097/MD.0000000000020892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328963PMC

Staphylococcus saccharolyticus infection: case series with a PRISMA-compliant systemic review.

Medicine (Baltimore) 2020 Jun;99(26):e20686

Department of Respiratory and Critical Care Medicine.

Background: Staphylococcus saccharolyticus is a rare cause of human infectious disease. The clinical characteristics and treatment of patients with S saccharolyticus infections remain largely unknown.

Objectives: We present the first reported case of empyema due to S saccharolyticus. Read More

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http://dx.doi.org/10.1097/MD.0000000000020686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329013PMC

Pre-operative methicillin resistant Staphylococcus aureus results do not predict surgical site infections in children undergoing varus derotational osteotomy.

Medicine (Baltimore) 2020 Jun;99(26):e20517

Keck School of Medicine, University of Southern California.

Literature regarding the value of pre-operative nasal methicillin resistant Staphylococcus aureus (MRSA) swabs to predict surgical site infections (SSIs) in children undergoing lower extremity surgery is limited. The purpose of our study was to determine if pre-operative nasal MRSA swab results were predictive of SSI development in children undergoing a femoral varus derotational osteotomy (VDRO).Patients who underwent VDRO between 2004-2016 were reviewed to determine pre-operative MRSA colonization rates and SSI devolvement rates. Read More

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http://dx.doi.org/10.1097/MD.0000000000020517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329002PMC
June 2020
5.723 Impact Factor

Lytic Activity of Polyvalent Staphylococcal Bacteriophage PhiSA012 and Its Endolysin Lys-PhiSA012 Against Antibiotic-Resistant Staphylococcal Clinical Isolates From Canine Skin Infection Sites.

Front Med (Lausanne) 2020 10;7:234. Epub 2020 Jun 10.

Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.

The spread of antibiotic-resistant bacteria (ARB) in human and veterinary medicine is of global concern. Notably, the emergence of methicillin-resistant has become a serious problem. In this context, bacteriophages and their lytic enzymes, endolysins, have received considerable attention as therapeutics for infectious diseases in place of antibiotics. Read More

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http://dx.doi.org/10.3389/fmed.2020.00234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298730PMC

Case report of a child with influenza and fatal community-associated methicillin-resistant Staphylococcus aureus sepsis.

Rev Soc Bras Med Trop 2020 22;53:e20200050. Epub 2020 Jun 22.

Universidade Federal de São Paulo, Departamento de Medicina, Laboratório Especial de Microbiologia Clinica, São Paulo, SP, Brasil.

In the present study, we report the incidence of septic shock syndrome associated with methicillin-resistant Staphylococcus aureus in a child who initially presented influenza-like illness and developed septic shock shortly after 48 h of hospitalization, and eventually died within a few hours of the onset of sepsis. S. aureus isolated from the blood culture was characterized as the community-associated strain carrying the staphylococcal cassette chromosome mec (SCCmec) type IV element. Read More

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http://dx.doi.org/10.1590/0037-8682-0050-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310360PMC

Nanomechanics of the molecular complex between staphylococcal adhesin SpsD and elastin.

Nanoscale 2020 Jul 24;12(26):13996-14003. Epub 2020 Jun 24.

Louvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, B-1348 Louvain-la-Neuve, Belgium.

Staphylococcus pseudintermedius surface protein SpsD binds to extracellular matrix proteins to invade canine epithelial cells. Using single-molecule experiments, we show that SpsD engages in two modes of interaction with elastin that are tightly controlled by physical stress. Binding is weak (∼100 pN) at low tensile force (i. Read More

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http://dx.doi.org/10.1039/d0nr02745fDOI Listing

Community-Acquired Methicillin-Resistant Staphylococcus aureus Infections in Acutely Ill Children: A Retrospective Case-Control Study.

Indian J Pediatr 2020 Jun 24. Epub 2020 Jun 24.

Division of Medical Microbiology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India.

Objectives: To test if admission clinical and laboratory variables could reliably discriminate community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections from methicillin-sensitive Staphylococcus aureus (MSSA) infections in acutely ill children, and to describe the epidemiology, clinical features and outcomes among children with Staphylococcal infections admitted to the hospital.

Methods: The authors conducted this retrospective case-control study comparing children with CA-MRSA and MSSA infections admitted to hospital between June 2014 and June 2017. They describe the evolving epidemiology and attempt to identify clinical and laboratory variables that can differentiate MRSA cases from MSSA controls. Read More

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http://dx.doi.org/10.1007/s12098-020-03391-zDOI Listing

Epidemiological and Clinical Evidence for the Role of Toxins in Human Disease.

Toxins (Basel) 2020 Jun 19;12(6). Epub 2020 Jun 19.

Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

asymptomatically colonizes approximately 30-50% of the population and is a leading cause of bacteremia, bone/joint infections, and skin infections in the US. has become a major public health threat due to antibiotic resistance and an increasing number of failed vaccine attempts. To develop new anti-staphylococcal preventive therapies, it will take a more thorough understanding of the current role virulence factors play in contributing to human disease. Read More

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http://dx.doi.org/10.3390/toxins12060408DOI Listing

Dynamics and conformational propensities of staphylococcal CntA.

J Biomol Struct Dyn 2020 Jun 23:1-13. Epub 2020 Jun 23.

Department of Bioinformatics, Hazara University, Mansehra, KPK, Pakistan.

Enzymes use transition metals as co-factors for catalytic roles in biological processes. Notably, manganese, iron, cobalt, nickel, copper and zinc are abundantly used. a commensal bacterium asymptomatically, lies on the human body causing variety of infections. Read More

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http://dx.doi.org/10.1080/07391102.2020.1782263DOI Listing

Minocycline Combined with Vancomycin for the Treatment of Methicillin-Resistant Coagulase-Negative Staphylococcal Prosthetic Joint Infection Managed with Exchange Arthroplasty.

J Bone Jt Infect 2020 12;5(3):110-117. Epub 2020 Apr 12.

Centre de Référence des Infections Ostéo-Articulaires Complexes, Groupe Hospitalier Diaconesses-Croix Saint-Simon, Paris, France.

Treatment of methicillin-resistant (MR) staphylococcal prosthetic joint infections (PJIs) remains a matter of discussion, with vancomycin-rifampin combination therapy being the preferred treatment for DAIR and one-stage exchange arthroplasty strategies. This study analyzes the outcomes of patients with chronic methicillin-resistant coagulase-negative staphylococcal PJIs treated with vancomycin-minocycline combination therapy. This prospective, single center cohort study included all chronic MR coagulase-negative staphylococcal PJIs (01/2004-12/2014) treated with exchange arthroplasty and at least 4 weeks of minocycline-vancomycin. Read More

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http://dx.doi.org/10.7150/jbji.43254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295649PMC

Prevalence and Molecular Genetics of Methicillin-Resistant Colonization in Nursing Homes in Saudi Arabia.

Can J Infect Dis Med Microbiol 2020 3;2020:2434350. Epub 2020 Jun 3.

Department of Pathology, School of Medicine, King Saud University, Riyadh, Saudi Arabia.

Objective: Methicillin-resistant (MRSA) is one of the main causative agents of nosocomial infections that has posed a major threat to those with compromised immune systems such as nursing home residents. The aim of this study was to determine the rates of MRSA strains and the types of )in nursing homes in Saudi Arabia.

Methods: A total of 188 nasal swabs were collected from the residents and nursing staff in two nursing homes in Riyadh, Saudi Arabia. Read More

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http://dx.doi.org/10.1155/2020/2434350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290903PMC

Antimicrobial Activity of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 Against Staphylococcus aureus Biofilms Isolated from Wound Infection.

Probiotics Antimicrob Proteins 2020 Jun 17. Epub 2020 Jun 17.

Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA.

Staphylococcal wound infections range from mild to severe with life-threatening complications. The challenge of controlling such infections is related to bacterial biofilm formation, which is a major factor contributing to antibiotic resistance and infection recurrence. In this study, four clinical isolates of staphylococci species; two isolates of methicillin-resistant Staphylococcus aureus (MRSA) and two methicillin-sensitive Staphylococcus aureus (MSSA) isolates. Read More

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http://dx.doi.org/10.1007/s12602-020-09673-4DOI Listing

Rifabutin Use in Biofilm Infections: A Case Series.

Antibiotics (Basel) 2020 Jun 13;9(6). Epub 2020 Jun 13.

Department of Medicine, University of Maryland Medical Center Midtown Campus, Baltimore, MD 21201, USA.

This is a case series of 10 patients who had staphylococcal biofilm infections that were treated with adjuvant rifabutin therapy instead of rifampin therapy. In these cases, rifampin was contraindicated secondary to drug-drug interactions with the patients' chronic medications. Rifabutin therapy was well tolerated with no side effects. Read More

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http://dx.doi.org/10.3390/antibiotics9060326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345564PMC

Innate Immune Response against Preincubated with Subinhibitory Concentration of -Anethole.

Int J Mol Sci 2020 Jun 11;21(11). Epub 2020 Jun 11.

Department of Laboratory Medicine, Chair of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.

The study aimed to analyze morphological and functional changes of cells due to -anethole (a terpenoid and the major constituent of fennel, anise, or star anise essential oils) exposition, and their consequences for human neutrophils phagocytic activity as well as IL-8 production (recognized as the major chemoattractant). The investigation included the evaluation of changes occurring in cultures, i.e. Read More

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http://dx.doi.org/10.3390/ijms21114178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312609PMC

Development and Evaluation of a Sensitive Bacteriophage-Based MRSA Diagnostic Screen.

Viruses 2020 Jun 11;12(6). Epub 2020 Jun 11.

Laboratory Corporation of America Holdings, New Brighton, MN 55112, USA.

Engineered luciferase reporter bacteriophages provide specific, sensitive, rapid and low-cost detection of target bacteria and address growing diagnostic needs in multiple industries. Detection of methicillin-resistant (MRSA) nasal colonization and antibiotic susceptibility play a critical supportive role in preventing hospital-acquired infections and facilitating antibiotic stewardship. We describe the development and evaluation of a novel phage-based MRSA diagnostic screen for nasal swab specimens. Read More

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http://dx.doi.org/10.3390/v12060631DOI Listing

Tryptic Shaving of Unveils Immunodominant Epitopes on the Bacterial Cell Surface.

J Proteome Res 2020 Jun 25. Epub 2020 Jun 25.

Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, P. O. Box 30001, 9700 RB Groningen, the Netherlands.

The opportunistic pathogen has become a major threat for human health and well-being by developing resistance to antibiotics and by fast evolution into new lineages that rapidly spread within the healthy human population. This calls for development of active or passive immunization strategies to prevent or treat acute phase infections. Since no such anti-staphylococcal immunization approaches are available for clinical implementation, the present studies were aimed at identifying new leads for their development. Read More

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http://dx.doi.org/10.1021/acs.jproteome.0c00043DOI Listing

Role of microRNAs in Staphylococcus aureus infection: Potential biomarkers and mechanism.

IUBMB Life 2020 Jun 9. Epub 2020 Jun 9.

Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Staphylococcus aureus is known as a common pathogen that colonizes 30% of healthy humans. Additionally, this bacterium can cause a number of serious infections, that is, endocarditis, bacteremia, pneumonia, wound, skin infections, and tissue abscesses. A variety of cellular and molecular pathways and targets are involved in response against S. Read More

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http://dx.doi.org/10.1002/iub.2325DOI Listing
June 2020
3.143 Impact Factor

Catheter-Associated Urinary Tract Infection, Clostridioides difficile Colitis, Central Line-Associated Bloodstream Infection, and Methicillin-Resistant Staphylococcus aureus.

Med Clin North Am 2020 Jul 12;104(4):663-679. Epub 2020 May 12.

The Division of Hospital Medicine, Department of Medicine, University of Michigan, 2800 Plymouth Road, Building 16 #432W, Ann Arbor, MI 48109, USA. Electronic address:

Hospital-acquired infections increase cost, morbidity, and mortality for patients across the United States and the world. Principal among these infections are central line-associated bloodstream infection, catheter-associated urinary tract infection, Clostridioides difficile, and methicillin-resistant Staphylococcus aureus colonization and infections. This article provides succinct summaries of the background, epidemiology, diagnosis, and treatment of these conditions. Read More

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http://dx.doi.org/10.1016/j.mcna.2020.02.004DOI Listing

[Negative pressure wound therapy for deep sternal wound infections: microbiological characteristics and antibiotic resistance].

Khirurgiia (Mosk) 2020 (5):34-41

Petrovsky Russian Research Center of Surgery, Moscow, Russia.

Objective: To evaluate bacterial flora in patients with deep sternal wound infection and the effect of negative pressure wound therapy on eradication of the pathogen.

Material And Methods: There were 102 patients with deep wound infection after cardiac surgery. Mean age was 66. Read More

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http://dx.doi.org/10.17116/hirurgia202005134DOI Listing

Prediction of skull base osteomyelitis in necrotising otitis externa with diffusion-weighted imaging.

J Laryngol Otol 2020 May 5;134(5):404-408. Epub 2020 Jun 5.

Department of Diagnostic Radiology, Mansoura University Faculty of Medicine, Mansoura, Egypt.

Objective: To predict skull base osteomyelitis in patients with necrotising otitis externa using diffusion-weighted imaging.

Methods: A retrospective analysis was conducted of 25 necrotising otitis externa patients with skull base osteomyelitis (n = 10) or without skull base involvement (n = 14) who underwent a single-shot diffusion-weighted imaging of the skull base.

Results: The respective mean apparent diffusion coefficient values of the skull base, as determined by two reviewers, were 0. Read More

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http://dx.doi.org/10.1017/S0022215120001073DOI Listing

Staphylococcal Vaccine Antigens related to biofilm formation.

Hum Vaccin Immunother 2020 Jun 4:1-11. Epub 2020 Jun 4.

Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science , Zanjan, Iran.

The number and frequency of multidrug-resistant (MDR) strains as a frequent cause of nosocomial infections have increased, especially for , in part due to device-related infections. The transition to antibiotic-resistance in related bacterial genes and the capability for immune escape have increased the sustainability of biofilms produced by these bacteria. The formation and changes in biofilms have been suggested as a target to prevent or treat staphylococcal infections. Read More

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http://dx.doi.org/10.1080/21645515.2020.1767449DOI Listing

Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non-refrigerated raw chicken livers.

Int J Food Microbiol 2020 Sep 20;328:108669. Epub 2020 May 20.

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections and staphylococcal food poisoning (SFP). This study was conducted to investigate the prevalence and enterotoxigenicity of MRSA in broiler chicken meat and giblets. A total of 5. Read More

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http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108669DOI Listing
September 2020

Potent, specific MEPicides for treatment of zoonotic staphylococci.

PLoS Pathog 2020 Jun 4;16(6):e1007806. Epub 2020 Jun 4.

Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri, United States of America.

Coagulase-positive staphylococci, which frequently colonize the mucosal surfaces of animals, also cause a spectrum of opportunistic infections including skin and soft tissue infections, urinary tract infections, pneumonia, and bacteremia. However, recent advances in bacterial identification have revealed that these common veterinary pathogens are in fact zoonoses that cause serious infections in human patients. The global spread of multidrug-resistant zoonotic staphylococci, in particular the emergence of methicillin-resistant organisms, is now a serious threat to both animal and human welfare. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297381PMC

Staphylococcus warneri: Skin Commensal and a Rare Cause of Urinary Tract Infection.

Cureus 2020 May 28;12(5):e8337. Epub 2020 May 28.

Internal Medicine, Indiana University Health, Indianapolis, USA.

Coagulase negative Staphylococci often grow in cultures and form one of the most abundant flora among skin microbiome. It is important and challenging to identify and treat clinically significant infections caused by these organisms. Prosthetic devices, catheters and conditions causing immunocompromised states are the risk factors for such infections. Read More

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http://dx.doi.org/10.7759/cureus.8337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263002PMC

Addressing prosthetic joint infections via gentamicin-eluting UHMWPE spacer.

Bone Joint J 2020 Jun;102-B(6_Supple_A):151-157

Harris Orthopaedic Laboratory, Massachusetts General Hospital.

Aims: We propose a state-of-the-art temporary spacer, consisting of a cobalt-chrome (CoCr) femoral component and a gentamicin-eluting ultra-high molecular weight polyethylene (UHMWPE) tibial insert, which can provide therapeutic delivery of gentamicin, while retaining excellent mechanical properties. The proposed implant is designed to replace conventional spacers made from bone cement.

Methods: Gentamicin-loaded UHMWPE was prepared using phase-separated compression moulding, and its drug elution kinetics, antibacterial, mechanical, and wear properties were compared with those of conventional gentamicin-loaded bone cement. Read More

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http://dx.doi.org/10.1302/0301-620X.102B6.BJJ-2019-1593.R1DOI Listing

Epidemiology and risk factors of Staphylococcus aureus CC398 bone and joint infections.

BMC Infect Dis 2020 May 29;20(1):384. Epub 2020 May 29.

Maladies infectieuses et tropicales, Centre Hospitalier Régional Universitaire, 25030, Besancon, France.

Background: A particular ability of the Staphylococcus aureus clonal complex 398 (CC398) to cause bone and joint infections (BJI) remains questionable, since some studies have described high prevalence of MSSA CC398 in prosthetic joint infection (PJI) and diabetic foot ostemolyelitis (DFO). Here, we described the long-term epidemiology of CC398 among S. aureus isolated from BJI and identified risk factors associated with CC398. Read More

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http://dx.doi.org/10.1186/s12879-020-05098-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260739PMC

Epidemiologic and Molecular Characteristics of Staphylococcus aureus Strains Isolated From Hospitalized Pediatric Patients.

Pediatr Infect Dis J 2020 May 26. Epub 2020 May 26.

Department of Infectious Diseases, Hacettepe University Faculty of Medicine, Ankara.

Background: We aimed to determine molecular characteristics of Staphylococcus aureus isolates cultured from hospitalized pediatric patients.

Methods: All accessible S. aureus isolates cultured from hospitalized pediatric patients were analyzed for staphylococcal cassette chromosome mec (SCCmec) types, Panton-Valentine Leukocidin (PVL) encoding genes and antibiotic resistance patterns. Read More

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http://dx.doi.org/10.1097/INF.0000000000002764DOI Listing

Enhanced bacterial killing by vancomycin in staphylococcal biofilms disrupted by novel, DMMA-modified carbon dots depends on EPS production.

Colloids Surf B Biointerfaces 2020 Sep 12;193:111114. Epub 2020 May 12.

University of Groningen and University Medical Center of Groningen Department of Orthodontics, Hanzeplein 1, 9700 RB Groningen, the Netherlands.

Alternatives for less and less effective antibiotic treatment of bacterial infections, are amongst others based on nanotechnological innovations, like carbon-dots. However, with a focus on chemistry, important characteristics of bacterial strains, like (in-)ability to produce extracellular-polymeric-substances (EPS) are often neglected. EPS is the glue that certain bacterial strains produce to keep a biofilm together. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2020.111114DOI Listing
September 2020
4.152 Impact Factor

Effect of itraconazole on Staphylococcus aureus biofilm and extracellular vesicles formation.

Microb Pathog 2020 May 25;147:104267. Epub 2020 May 25.

Institute of Clinical Physiology, CNR, Pisa, Italy. Electronic address:

Staphylococcus aureus is a leading cause of a wide range of clinical chronic infections mainly due to the establishment of a biofilm. Biofilm, a population of bacteria within a self-produced matrix of extracellular polymeric substance, decreases the susceptibility to antibiotics, immune defenses and contributes to antimicrobial resistance. To date antibiotic combination has been considered a strategy to combat S. Read More

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http://dx.doi.org/10.1016/j.micpath.2020.104267DOI Listing

Complete genome sequence of a methicillin-resistant Staphylococcus lugdunensis strain and characteristics of its staphylococcal cassette chromosome mec.

Sci Rep 2020 May 26;10(1):8682. Epub 2020 May 26.

Center of Excellence for Infection Control Science, Graduate School of Medicine, Juntendo University, Tokyo, Japan.

Symptoms of Staphylococcus lugdunensis infection are often similar to those of Staphylococcus aureus infection, including skin and soft-tissue lesions, bacteremia and infective endocarditis. Despite the severity of these infections, S. lugdunensis is regarded as a less important pathogen than drug-resistant S. Read More

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http://dx.doi.org/10.1038/s41598-020-65632-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251135PMC

Roles of the Site 2 Protease Eep in Staphylococcus aureus.

J Bacteriol 2020 Jul 9;202(15). Epub 2020 Jul 9.

Department of Laboratory Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

In , the site 2 protease Eep generates sex pheromones, including cAM373. Intriguingly, in , a peptide similar to cAM373, named cAM373_SA, is produced from the gene. Here, we report that the staphylococcal Eep homolog is not only responsible for the production of cAM373_SA but also critical for staphylococcal virulence. Read More

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http://dx.doi.org/10.1128/JB.00046-20DOI Listing
July 2020
2.808 Impact Factor

Assessment of a Multiplex Serological Test for the Diagnosis of Prosthetic Joint Infection: a Prospective Multicentre Study.

J Bone Jt Infect 2020 30;5(2):89-95. Epub 2020 Mar 30.

Bacteriology Department, CHU Nantes, Nantes Université, Nantes, France.

The diagnosis of prosthetic joint infections (PJIs) can be difficult in the chronic stage and is based on clinical and paraclinical evidence. A minimally invasive serological test against the main pathogens encountered during PJI would distinguish PJI from mechanical loosening. We performed a prospective, multicentre, cross-sectional study to assess the contribution of serology in the diagnosis of PJI. Read More

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http://dx.doi.org/10.7150/jbji.42076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242409PMC

Glycolytic inhibitor 2-deoxyglucose suppresses inflammatory response in innate immune cells and experimental staphylococcal endophthalmitis.

Exp Eye Res 2020 May 23;197:108079. Epub 2020 May 23.

Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University, Detroit, MI, USA; Department of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI, USA; Graduate School, Wayne State University, Detroit, MI, USA. Electronic address:

Previously, we have shown that Staphylococcus (S) aureus induces a glycolytic response in retinal residential (microglia) and infiltrated cells (neutrophils and macrophages) during endophthalmitis. In this study, we sought to investigate the physiological role of glycolysis in bacterial endophthalmitis using a glycolytic inhibitor, 2-deoxyglucose (2DG). Our data showed that 2DG treatment attenuated the inflammatory responses of mouse bone marrow-derived macrophages (BMDM) and neutrophils (BMDN) when challenged with either live or heat-killed S. Read More

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http://dx.doi.org/10.1016/j.exer.2020.108079DOI Listing
May 2020
2.709 Impact Factor

In Vitro Antimicrobial Activity of a New Ophthalmic Solution Containing Hexamidine Diisethionate 0.05% (Keratosept).

Cornea 2020 May 25. Epub 2020 May 25.

Department of Biomedical Sciences, University of Sassari, Sassari, Italy.

Purpose: To assess the in vitro antimicrobial activity of a new commercial ophthalmic solution containing hexamidine diisethionate 0.05% (Keratosept).

Methods: Staphylococcus aureus American Type Culture Collection (ATCC 43300), Pseudomonas aeruginosa ATCC 27853, 3 ocular bacterial isolates (1 Staphylococcus epidermidis, 1 S. Read More

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http://dx.doi.org/10.1097/ICO.0000000000002375DOI Listing

Dual Surgical Setup May Improve Infection Control Rate of Debridement and Implant Retention Procedures for Periprosthetic Infections of the Hip and Knee.

J Arthroplasty 2020 Apr 27. Epub 2020 Apr 27.

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA; Department of Orthopaedic Surgery, Newton-Wellesley Hospital, Newton, MA.

Background: Debridement, antibiotics, and implant retention (DAIR) is an appealing treatment option for periprosthetic joint infection (PJI) due to its low cost and low morbidity. There are many nonmodifiable risk factors for DAIR failure that have previously been established. A dual DAIR setup constitutes establishing a new, sterile field after the initial debridement. Read More

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http://dx.doi.org/10.1016/j.arth.2020.04.068DOI Listing

Multidrug-resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolated from a subtropical river contaminated by nearby livestock industries.

Ecotoxicol Environ Saf 2020 Sep 22;200:110724. Epub 2020 May 22.

Department of Earth and Environmental Sciences, National Chung Cheng University, 168 University Road, Minhsiung Township, Chiayi County, 62102, Taiwan, ROC, Taiwan, ROC.

Methicillin-resistant Staphylococcus aureus (MRSA) is a major threat to public health that causes infections in hospitals, communities, and animal husbandry. Livestock-associated MRSA (LA-MRSA) is defined as MRSA possessing staphylococcal cassette chromosome mec (SCCmec) IV or V, both of which lacks the Panton-Valentine leukocidin (PVL) gene but has variable combinations of antimicrobial susceptibility. This study focused on Taiwan's subtropical river basin and the Puzih River, which converges from tributaries flowing through downtown and animal husbandry areas. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2020.110724DOI Listing
September 2020
2.762 Impact Factor