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Microbiol Spectr 2022 Jun 27:e0227221. Epub 2022 Jun 27.

Toho University, Tokyo, Japan.

Although community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged worldwide, no nationwide CA-MRSA surveillance has been conducted in Japan to determine the changes in its molecular characteristics over time. We aimed to characterize the molecular epidemiology of Panton-Valentine leucocidin (PVL)-positive CA-MRSA strains collected from across Japan in the past decade. We isolated 1,770 MRSA strains from the skin and pus samples of outpatients of 244 medical facilities in 31 prefectures between 2010 and 2018 (2010, 2012, 2014, 2016, and 2018). Read More

Microbiol Spectr 2022 Jun 27:e0064022. Epub 2022 Jun 27.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Konggrid.194645.b, Pok Fu Lam, Hong Kong.

Antibiotics are widely used for the treatment of bacterial infections. However, injudicious use of antibiotics based on an empirical method may lead to the emergence of resistant strains. Despite appropriate administration of antibiotics, their concentrations may remain subinhibitory in the body, due to individual variations in tissue distribution and metabolism rates. Read More

Front Microbiol 2022 9;13:930629. Epub 2022 Jun 9.

Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Antibiotics play a vital role in saving millions of lives from fatal infections; however, the inappropriate use of antibiotics has led to the emergence and propagation of drug resistance worldwide. Multidrug-resistant bacteria represent a significant challenge to treating infections due to the limitation of available antibiotics, necessitating the investigation of alternative treatments for combating these superbugs. Under such circumstances, antimicrobial peptides (AMPs), including human-derived AMPs and bacteria-derived AMPs (so-called bacteriocins), are considered potential therapeutic drugs owing to their high efficacy against infectious bacteria and the poor ability of these microorganisms to develop resistance to them. Read More

Yonsei Med J 2022 Jul;63(7):611-618

Department of Internal Medicine, Division of Infectious Disease, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea.

Purpose: This study aimed to provide compelling evidence of anti-staphylococcal beta-lactam use for methicillin-susceptible bloodstream infection (MSSA BSI).

Materials And Methods: We retrospectively collected data on patients with MSSA BSI who were admitted to two academic tertiary-care hospitals from 2010 to 2018. Only patients who received nafcillin, cefazolin, vancomycin, or teicoplanin as definitive therapy were included. Read More

Interdiscip Perspect Infect Dis 2022 14;2022:6503929. Epub 2022 Jun 14.

Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

Clindamycin can serve as an alternative treatment for staphylococcal infections. Routine susceptibility tests may fail to determine inducible type clindamycin resistance and can be a source of failure in clinical therapeutics. Therefore, this study aimed to determine () prevalence, inducible clindamycin resistance pattern, and associated factors among patients attending the University of Gondar Comprehensive Specialized Hospital, Gondar, northwest Ethiopia. Read More

Viruses 2022 Jun 20;14(6). Epub 2022 Jun 20.

Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Center for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, Germany.

has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complicated by specific antibiotic resistance genes and the formation of biofilms. Hence, novel therapeutic strategies are needed to fight these infections. Read More

Viruses 2022 May 31;14(6). Epub 2022 May 31.

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.

The study of bacteriophages is experiencing a resurgence owing to their antibacterial efficacy, lack of side effects, and low production cost. Nonetheless, the interactions between bacteriophages and their hosts remain unexplored. In this study, whole-genome sequences of 188 bacteriophages-20 , 56 , and 112 -were obtained from the National Center for Biotechnology Information (NCBI, USA) genome database. Read More

Int J Mol Sci 2022 Jun 9;23(12). Epub 2022 Jun 9.

MML Centre, Bagno 2, 00-112 Warsaw, Poland.

  is an opportunistic pathogen found in the healthy human skin microbiome bacterial community that is able to cause infections of diverse localization, manifestation, and course, including laryngological infections, such as necrotizing sinusitis. Chronic maxillary sinusitis is a disease present in up to one third of European and American populations, and its etiology is not fully described. Within this study, we aimed to characterize 18 strains recovered from maxillary sinuses and evaluate them as etiological agents of chronic disease. Read More

Int J Mol Sci 2022 Jun 9;23(12). Epub 2022 Jun 9.

Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Krakow, 30-387 Krakow, Poland.

Whilst a large number of regulatory mechanisms for gene expression have been characterised to date, transcription regulation in bacteria still remains an open subject. In clinically relevant and opportunistic pathogens, such as , transcription regulation is of great importance for host-pathogen interactions. In our study we investigated an operon, exclusive to staphylococci, that we name . Read More

Int J Mol Sci 2022 Jun 8;23(12). Epub 2022 Jun 8.

Surgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, Australia.

is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infected devices. In this study, we performed in-solution digestion followed by TMT-based high-throughput mass spectrometry and investigated what changes occur in the proteome of biofilm grown for 3-days and 12-days in comparison with 24 h planktonic. Read More

Antibiotics (Basel) 2022 Jun 17;11(6). Epub 2022 Jun 17.

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

can cause a plethora of life-threatening infections. Antibiotics have been extensively used to treat infections. However, when antibiotics are used at sub-inhibitory concentrations, especially for β-lactam antibiotics, they may enhance staphylococcal pathogenicity and exacerbate the infection. Read More

Antibiotics (Basel) 2022 May 27;11(6). Epub 2022 May 27.

Yale Department of Dermatology, Yale School of Medicine, New Haven, CT 06520, USA.

Tetracycline class antibiotics are widely used for multiple skin diseases, including acne vulgaris, acne rosacea, cutaneous infections, inflammatory dermatoses, and autoimmune blistering disorders. Concerns about antibiotic resistance and protecting the human/host microbiome beg the question whether broad-spectrum tetracyclines such as doxycycline and minocycline should be prescribed at such a high rate by dermatologists when a narrow-spectrum tetracycline derivative, sarecycline, exists. We evaluated the clinical effectiveness of oral sarecycline against cutaneous staphylococcal infections, eyelid stye, and mucous membrane pemphigoid to determine whether sarecycline is a viable option for clinicians to practice improved antibiotic stewardship. Read More

Commun Biol 2022 Jun 23;5(1):618. Epub 2022 Jun 23.

Department of Laboratory Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Pneumonia caused by community-associated Staphylococcus aureus (CA-SA) has high morbidity and mortality, but its pathogenic mechanism remains to be further investigated. Herein, we identify that staphylokinase (SAK) is significantly induced in CA-SA and inhibits biofilm formation in a plasminogen-dependent manner. Importantly, SAK can enhance CA-SA-mediated pneumonia in both wild-type and cathelicidins-related antimicrobial peptide knockout (CRAMP) mice, suggesting that SAK exacerbates pneumonia in a CRAMP-independent manner. Read More

PLoS One 2022 23;17(6):e0270393. Epub 2022 Jun 23.

Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, Hanover, New Hampshire, United States of America.

Patients with cystic fibrosis (CF) often suffer recurrent bronchial bacterial infections that lead to deterioration of lung function over time. The infections in CF patients are often due to S. aureus and P. Read More

Vet Sci 2022 Jun 16;9(6). Epub 2022 Jun 16.

Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine Timișoara, Calea Aradului 119, 300645 Timișoara, Romania.

Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of strains to exhibit primary resistance to various drugs in vitro is increasing. Read More

Ned Tijdschr Geneeskd 2022 05 4;166. Epub 2022 May 4.

Albert Schweitzer Ziekenhuis, afd. Kindergeneeskunde, Dordrecht.

Background: An umbilical infection (omphalitis) is frequent in de neonatal period. The infection usually presents as a relatively mild cellulitis. However, in rare cases omphalitis has a complicated course. Read More

J Bacteriol 2022 Jun 23:e0061721. Epub 2022 Jun 23.

Department of Pathology and Microbiology, University of Nebraska Medical Centergrid.266813.8, Omaha, Nebraska, USA.

Staphylococcus aureus is a medically important pathogen with high metabolic versatility allowing it to infect various niches within a host. S. aureus utilizes two major transcriptional regulators, namely, CodY and CcpA, to remodel metabolic and virulence gene expression in response to changing environmental conditions. Read More

Pan Afr Med J 2022 30;41:259. Epub 2022 Mar 30.

Department of Biotechnology, Faculty of Agriculture, University for Development Studies, Tamale, Ghana.

Introduction: mobile phone plays an essential role in the lives of healthcare professionals in hospitals as far as communication is concerned. However, it can also serve as a source of nosocomial infections. This study aimed at determining the prevalence and antibiotic susceptibility of Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli isolated from mobile phones used by healthcare staff working in three public hospitals in Ghana. Read More

Sci Rep 2022 Jun 22;12(1):10541. Epub 2022 Jun 22.

Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Whole genome sequencing (WGS) of methicillin-resistant Staphylococcus aureus (MRSA) provides high-resolution typing, facilitating surveillance and outbreak investigations. The aim of this study was to evaluate the genomic variation rate in MRSA, by comparing commonly used core genome multilocus sequencing (cgMLST) against single nucleotide polymorphism (SNP) analyses. WGS was performed on 95 MRSA isolates, collected from 20 carriers during years 2003-2019. Read More

BMJ Case Rep 2022 Jun 22;15(6). Epub 2022 Jun 22.

Oncology & Metabolism, Univerisity of Sheffield, Sheffield, UK

A man in his 50s with type 2 diabetes mellitus (T2DM) presented with a nasal septal abscess 3 weeks following septoplasty. Diabetes mellitus has been reported in association with nasal septal abscess, thought to be due to a relative immunodeficient state. We present an unusual, delayed presentation of nasal septal abscess following septoplasty and performed a literature review. Read More

Microbiol Spectr 2022 Jun 22:e0034222. Epub 2022 Jun 22.

Division IV of Food Safety Standards, China National Center for Food Safety Risk Assessmentgrid.464207.3, Beijing, China.

Sequence type 88 (ST88) methicillin-resistant Staphylococcus aureus (MRSA) has been recognized as an important pathogen that causes infections in humans, especially when it has strong biofilm production and multidrug resistance (MDR). However, knowledge of the determinants of resistance or virulence and genomic characteristics of ST88 MRSA from China is still limited. In this study, we employed the antimicrobial resistance (AMR), biofilm formation, and genomic characteristics of ST88 MRSA collected from various foods in China and estimated the worldwide divergence of ST88 MRSA with publicly available ST88 genomes. Read More

MAbs 2022 Jan-Dec;14(1):2083467

Integrated BioTherapeutics, Rockville, USA.

carries an exceptional repertoire of virulence factors that aid in immune evasion. Previous single-target approaches for -specific vaccines and monoclonal antibodies (mAbs) have failed in clinical trials due to the multitude of virulence factors released during infection. Emergence of antibiotic-resistant strains demands a multi-target approach involving neutralization of different, non-overlapping pathogenic factors. Read More

Am J Case Rep 2022 Jun 22;23:e936058. Epub 2022 Jun 22.

Department of Emergency Medicine, Yamato Tokushukai Hospital, Yamato, Kanagawa, Japan.

BACKGROUND Vertebral osteomyelitis is a rare form of bone infection that requires prompt diagnosis and treatment; however, this is challenging because of the lack of specific symptoms and low sensitivity of diagnostic tests, especially in the early stages. Our case demonstrates the challenges in diagnosing vertebral osteomyelitis and provides relevant information for other physicians dealing with possible cases of vertebral osteomyelitis. CASE REPORT An 83-year-old man presented to the Emergency Department with severe low back pain of 2 weeks' duration. Read More

Microbiol Spectr 2022 Jun 21:e0241321. Epub 2022 Jun 21.

Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.

Infections caused by antibiotic-resistant are a global concern. This is true in the Middle East, where increasingly resistant Staphylococcus aureus and Staphylococcus haemolyticus strains have been detected. While extensive surveys have revealed the prevalence of infections caused by antibiotic-resistant staphylococci in Europe, Asia, and North America, the population structure of antibiotic-resistant staphylococci recovered from patients and clinical settings in Egypt remains uncharacterized. Read More

J Investig Med High Impact Case Rep 2022 Jan-Dec;10:23247096221105243

Department of Internal Medicine, Kern Medical, Bakersfield, CA, USA.

Erythrodermic psoriasis is a rare subtype of psoriasis vulgaris that presents with diffuse erythema and desquamation over greater than 75% of the body's surface area. We present a case of a 57-year-old male who was admitted with a diffuse, erythematous scaly rash covering his entire body, with associated subjective fevers. Skin biopsy revealed erythrodermic psoriasis, and blood cultures were positive for methicillin-sensitive . Read More

Assay Drug Dev Technol 2022 May-Jun;20(4):175-182

College of Pharmaceutical Science, Collaborative Innovation Center of Yangtza River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, China.

Staphylococcus aureus (MRSA) are challenging pathogenic bacteria that can cause severe infection leading to high mortality rates. We found that both the oxacillin- and cefoxitin-resistant strains isolated from clinic showed multidrug-resistant (MDR) characteristics. Through rapid high-throughput screen (HTS) of a compound library, gemcitabine and selen compounds were found to effectively inhibit growth. Read More

J Vis Exp 2022 Jun 1(184). Epub 2022 Jun 1.

Department of Molecular Biology and Biochemistry, University of California Irvine;

Volatile organic compounds (VOCs) from biological samples have unknown origins. VOCs may originate from the host or different organisms from within the host's microbial community. To disentangle the origin of microbial VOCs, volatile headspace analysis of bacterial mono- and co-cultures of Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii, and stable isotope probing in biological samples of feces, saliva, sewage, and sputum were performed. Read More

BMC Infect Dis 2022 Jun 17;22(1):555. Epub 2022 Jun 17.

Centre de Référence des Infections Ostéo-Articulaires Complexes, Groupe Hospitalier Diaconesses-Croix Saint-Simon, 125, rue d'Avron, 75020, Paris, France.

Background: Staphylococci and streptococci are the most frequent pathogens isolated from prosthetic joint infections (PJIs). The aim of this study was to analyze the outcome of streptococcal and methicillin-susceptible Staphylococcus aureus (MSSA) PJIs.

Methods: All monomicrobial streptococcal and MSSA PJIs managed in a French Referral Center (2010-2017) were sampled from the prospective PJIs cohort study. Read More

Sci Rep 2022 Jun 17;12(1):10161. Epub 2022 Jun 17.

Department of Midwifery, College of Medicine and Health Sciences, Injibara University, Injibara, Ethiopia.

Individuals with Methicillin-Resistant Staphylococcus aureus (MRSA) colonized nasal cavities were at greater risk of developing the infection and can serve as potential reservoirs of transmission. Aim of this study is to determine the extent of nasal carriage and associated factors linked to MRSA in medical and health science students of Arba Minch University (AMU), Ethiopia, who are much prone. An institution based cross-sectional study was conducted at AMU from 01st August through 30th November, 2020 by means of a systematic sampling technique using a structured questionnaire. Read More

Front Cell Infect Microbiol 2022 30;12:895022. Epub 2022 May 30.

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.

The leading cause of treatment failure in infections is the development of biofilms. Biofilms are highly tolerant to conventional antibiotics which were developed against planktonic cells. Consequently, there is a lack of antibiofilm agents in the antibiotic development pipeline. Read More