2,949 results match your criteria Skin Tissue Expansion


[Clinical effect of pre-expanded deltopectoral flap in the repair of faciocervical lesion and defect].

Zhonghua Shao Shang Za Zhi 2020 May;36(5):363-369

Department of Plastic Surgery, the First Affiliated Hospital, Air Force Medical University, Xi'an 710032, China.

To explore the clinical effect of pre-expanded deltopectoral flap in the repair of faciocervical lesion and defect. From July 2004 to August 2018, 355 patients with faciocervical lesion and defect were admitted to the First Affiliated Hospital of Air Force Medical University, including 200 males and 155 females aged 4 to 48 years with major conditions including thermal burn scars, and type Ⅲ and Ⅳ facial-cervical deformities. During the stage Ⅰ skin soft tissue expander implantation surgery, according to the size and location of lesion and defect, expanders with appropriate volume were placed to expand the deltopectoral area. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn501120-20200113-00019DOI Listing

Free tissue transfer for burns reconstruction: A single-site experience.

Burns 2020 Apr 24. Epub 2020 Apr 24.

Department of Plastic and Reconstructive and Burns Surgery, Salisbury NHS Foundation Trust, Odstock Road, Salisbury SP2 8BJ, United Kingdom.

This study reviews free tissue transfer (FTT) surgery for both acute wound and reconstructive scar management of burn injuries at a UK burns unit over a 10-year period. Thirty eight patients underwent 46 FTTs, or free flaps, as part of their burn injury pathway. For the cohort of patients, there was one flap failure, which occurred for a secondary scar reconstruction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.burns.2020.04.001DOI Listing

Thermal injury initiates pervasive fibrogenesis in skeletal muscle.

Am J Physiol Cell Physiol 2020 May 20. Epub 2020 May 20.

Department of Athletic Training and Clinical Nutrition, University of Kentucky, Lexington, KY, USA; Center for Muscle Biology, University of Kentucky, Lexington, Kentucky, USA; Shriners Hospitals for Children, Galveston, TX, USA.

Severe burn injury induces a myriad of deleterious effects to skeletal muscle, resulting in impaired function and delayed recovery. Following burn, catabolic signaling and myofiber atrophy are key fiber-intrinsic determinants of weakness; less well understood are alterations in the interstitial environment surrounding myofibers. Muscle quality, specifically alterations in the extracellular matrix (ECM), modulates force transmission and strength. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajpcell.00337.2019DOI Listing
May 2020
3.780 Impact Factor

From acute management to ultimate reconstruction: a 15-year follow-up of a pediatric patient with necrotizing fasciitis secondary to varicella infection.

Ann Burns Fire Disasters 2019 Dec;32(4):331-334

American University of Beirut Medical Center, Beirut, Lebanon.

Necrotizing fasciitis is a rapidly progressive, life-threatening soft tissue infection. The usually aggressive surgical debridement often leaves disfiguring sequelae.We hereby present the case of a 4-yearold boy who presented with post varicella zoster necrotizing fasciitis over his trunk, and follow his journey post reconstruction with split thickness skin grafts and the sequelae of grafting over the trunk. Read More

View Article

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197905PMC
December 2019

Establishment of a Novel Mouse Model for Soft Tissue Expansion.

J Surg Res 2020 May 5;253:238-244. Epub 2020 May 5.

Department of Plastic Surgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi Province, China. Electronic address:

Background: Despite its increasing use, not much is known about tissue expansion, and its complication rates are significantly high. Thus, there is an urgent need to establish a stable animal model to overcome the limitations and complications of tissue expansion. Although the mouse model has shown several advantages in the in-depth studies, an appropriate mouse expansion model has rarely been reported, likely because of its loose skin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2020.03.005DOI Listing

Non-invasive delivery of biological macromolecular drugs into the skin by iontophoresis and its application to psoriasis treatment.

J Control Release 2020 Apr 28;323:323-332. Epub 2020 Apr 28.

Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi 1, Tokushima 770-8505, Japan. Electronic address:

Biological macromolecular drugs, such as antibodies and fusion protein drugs, have been widely employed for the treatment of various diseases. Administration routes are typically via invasive intravenous or subcutaneous injection with needles; the latter is challenging for applications involving inflamed skin (e.g. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2020.04.044DOI Listing

Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold.

Biomolecules 2020 Apr 28;10(5). Epub 2020 Apr 28.

Tissue Engineering Research Laboratory, Department of Bioengineering, IQS-School of Engineering, Ramon Llull University, 08017 Barcelona, Spain.

Hair follicle dermal papilla cells (HFDPC) are a specialized cell population located in the bulge of the hair follicle with unique characteristics such as aggregative behavior and the ability to induce new hair follicle formation. However, when expanded in conventional 2D monolayer culture, their hair inductive potency is rapidly lost. Different 3D culture techniques, including cell spheroid formation, have been described to restore, at least partially, their original phenotype, and therefore, their hair inductive ability once transplanted into a recipient skin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom10050684DOI Listing

Consensus on Contouring Primary Breast Tumors on MRI in the Setting of Neoadjuvant Partial Breast Irradiation in Trials.

Pract Radiat Oncol 2020 Apr 18. Epub 2020 Apr 18.

Department of Radiation Oncology, Amsterdam University Medical Center, Amsterdam, the Netherlands.

Purpose: Our purpose was to present and evaluate expert consensus on contouring primary breast tumors on magnetic resonance imaging (MRI) in the setting of neoadjuvant partial breast irradiation in trials.

Methods And Materials: Expert consensus on contouring guidelines for target definition of primary breast tumors on contrast-enhanced MRI in trials was developed by an international team of experienced breast radiation oncologists and a dedicated breast radiologist during 3 meetings. At the first meeting, draft guidelines were developed through discussing and contouring 2 cases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.prro.2020.03.011DOI Listing

Two-stage surgical treatment of giant congenital melanocytic nevus around the auricle.

J Cosmet Dermatol 2020 Apr 21. Epub 2020 Apr 21.

Department of Auricular Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: This study presents the results of complete excision of giant congenital melanocytic nevi (GCMN) on the auricle, forehead, or periorbital area combined with tissue expansion, and skin graft transplantation performed in two stages based on 10 years of experience.

Aims: To solve the giant congenital melanocytic nevi on the auricle, forehead, or periorbital area with two-stage operation.

Patients/methods: A total of 21 patients with GCMN were included in this study. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jocd.13416DOI Listing

The new bioabsorbable sheet for the sling method in immediate breast reconstruction with expander-implant: a study protocol for interventional prospective study.

Nagoya J Med Sci 2020 Feb;82(1):39-45

Department of Plastic and Reconstructive Surgery, Okayama University Hospital.

The popularity of a sling method using biomaterial sheets for immediate breast reconstruction based on prosthesis has been increasing in western countries. However, acellular dermal matrix, which is representative of the biomaterial sheet, is not available and the sling method also has not been accepted in Japan. We focused on a new bioabsorbable sheet (NEOVEIL sheet) as a substitute for the sling method and report a prospective study protocol to assess the safety and effectiveness of this material. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.18999/nagjms.82.1.39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103866PMC
February 2020

Platelet lysate promotes the expansion of T regulatory cells that favours in vitro wound healing by increasing keratinocyte migration and fibroblast production of extracellular matrix components.

Eur J Dermatol 2020 Feb;30(1):3-11

National Institute for Health, Migration and Poverty, INMP, via di S.Gallicano, 25, Roma, Italy.

Background: Platelet lysate (PL) contains a cocktail of growth factors and cytokines that promote tissue repair and regeneration. In vitro studies have shown that PL may affect the reparative function of keratinocytes and fibroblasts, but little is known about the effect of PL on immune cells involved in wound healing.

Objectives: To analyse the effects of PL on T cells involved in the wound repair process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1684/ejd.2020.3711DOI Listing
February 2020

Centriole Number and the Accumulation of Microtubules Modulate the Timing of Apical Insertion during Radial Intercalation.

Curr Biol 2020 May 2;30(10):1958-1964.e3. Epub 2020 Apr 2.

Department of Cell and Developmental Biology, Lurie Comprehensive Cancer Center, Northwestern University, Feinberg School of Medicine, 303 E. Chicago Ave, Chicago, IL 60611, USA. Electronic address:

Centrioles are microtubule (MT)-based structures that provide important functions during cell migration, cell division, and cell signaling [1]. Modulating centriole number in 3D cell cultures has been shown to influence protrusive behavior [2-5]. Here, we address in vivo the role of centrioles and the accumulation of MTs on the protrusive behavior required during the initiation of radial intercalation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cub.2020.03.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239760PMC

Poly-4-Hydroxybutyric Acid Mesh Compares Favorably With Acellular Dermal Matrix in Tissue Expander-Based Breast Reconstruction.

Ann Plast Surg 2020 Apr 1. Epub 2020 Apr 1.

From the NewYork-Presbyterian Hospital/Weill Cornell Medicine, New York, NY.

Background: Acellular dermal matrix (ADM) is commonly used during immediate expander-based breast reconstruction, with potential advantages of greater intraoperative expansion, decreased time to complete expansion, and decreased rates of capsular contracture. However, ADM is associated with increased infection rate, seroma, and subsequent reconstructive failure. Poly-4-hydroxybutyric acid (P4HB) mesh is a large pore, biosynthetic scaffold shown to fully resorb and incorporate host tissues within 18 months. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/SAP.0000000000002339DOI Listing

[Cutaneous Manifestations in Mastocytosis: Update].

Acta Med Port 2020 Apr 1;33(4):275-281. Epub 2020 Apr 1.

Serviço de Dermatologia. Hospital de Santo António. Centro Hospitalar e Universitário do Porto. Porto. Unidade de Investigação em Dermatologia. Hospital de Santo António. Centro Hospitalar e Universitário do Porto. Porto. Portugal.

Introduction: Mastocytosis is characterized by the clonal expansion of morphological and immunophenotypically abnormal mast cells in different organs. The skin is the most frequently affected tissue. Virtually all children and more than 80% of adult patients with mastocytosis show cutaneous lesions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.20344/amp.12189DOI Listing

Reconstruction of post-burn anterior neck contractures using a butterfly design free anterolateral thigh perforator flap.

Arch Plast Surg 2020 Mar 15;47(2):194-197. Epub 2020 Mar 15.

Department of Plastic Reconstructive Surgery, European Georges Pompidou Hospital (AP-HP), Paris Descartes University, Paris, France.

Anterior neck burns represent a major reconstructive challenge due to severe sequalae including restriction in movement and poor aesthetic outcomes. Common treatment options include skin grafting with/without dermal matrices, and loco-regional and distant free flap transfers with/without prior tissue expansion. Such variation in technique is largely influenced by the extent of burn injury requiring resurfacing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.5999/aps.2019.00591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093264PMC

[Dose Distribution Combinations of Different Electron Beam Energy for Treatment Region Expansion in High-energy Electron Beam Radiation Therapy: A Feasibility Study].

Nihon Hoshasen Gijutsu Gakkai Zasshi 2020 ;76(3):259-269

Department of Radiotherapy, Nagoya University Hospital.

Introduction: External electron beams have excellent distributions in treatment for superficial tumors while suppressing influence deeper normal tissue. However, the skin surface cannot be given a sufficient dose due to the build-up effect. In this study, we have investigated the combination of electron beams to expand the treatment region by keeping the dose gradient beyond d. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.6009/jjrt.2020_JSRT_76.3.259DOI Listing
January 2020

Directional dependent variation in mechanical properties of planar anisotropic porcine skin tissue.

J Mech Behav Biomed Mater 2020 Apr 11;104:103693. Epub 2020 Feb 11.

Department of Mechanical Engineering, Indian Institute of Technology Ropar, Rupnagar, 140001, Punjab, India. Electronic address:

Nonlinear and anisotropic mechanical behavior of skin is essential in various applications such as dermatology, cosmetic products, forensic science, and computational studies. The present study quantifies the mechanical anisotropy of skin using the bulge method and full-field imaging technique. In bulging, the saline solution at 37 °C mimics the in vivo body temperature and fluid conditions, and all experiments were performed in the control environment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmbbm.2020.103693DOI Listing

Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing.

Front Bioeng Biotechnol 2020 21;8:110. Epub 2020 Feb 21.

Stem Cell and Tissue Engineering Lab, Institute of Health Service and Transfusion Medicine, Beijing, China.

Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.00110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046802PMC
February 2020

ECDI-fixed donor splenocytes prolong skin allograft survival by promoting M2 macrophage polarization and inducing regulatory T cells.

FASEB Bioadv 2019 Nov 17;1(11):706-718. Epub 2019 Oct 17.

Department of Immunology Fourth Military Medical University Xi'an China.

Rejection is a common complication of allogeneic tissue transplantation. Fixation of splenocytes (SP) with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) induces immune tolerance in recipients post-transplantation; however, the mechanism underlying this effect remains unclear. Here, we determined the mechanisms of ECDI-fixed donor SP (ECDI-SP) in inducing tolerance in skin allograft transplantation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fba.2019-00029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996306PMC
November 2019

Reconstruction of burn contractures of the anterior neck with pre-expanded free anterolateral thigh flaps.

Injury 2020 Feb 24. Epub 2020 Feb 24.

University of Pittsburgh, Department of Plastic Surgery, 3550 Terrace Street/6B Scaife Hall, Pittsburgh, PA 15261, USA.

Burn contractures of the anterior neck result in severe functional and aesthetic deformities. The release of wide contractures leads to defects that cannot be closed with local flaps. In these cases, tissue expansion of local tissues may be a solution. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.injury.2020.02.112DOI Listing
February 2020

Isolating stem cells from skin: designing a novel highly efficient non-enzymatic approach.

Physiol Res 2019 12;68(Suppl 4):S385-S388

Department of Biomedical Sciences, University of Sassari, Sassari, Italy.

Stem cells are undifferentiated elements capable to acquire a specific cellular phenotype under the influence of specific stimuli, thus being involved in tissue integrity and maintenance. In the skin tissue self-renewal and wound healing after injury is a complex process, especially in adulthood, due to the aging process and the continuous exposure to damaging agents. The importance of stem cells in regenerative medicine is well known and defining or improving their isolation methods is therefore a primary and crucial step. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.33549/physiolres.934373DOI Listing
December 2019

[Tissue expansion in child's burn reconstruction. Management principles and eleven years retrospective study of 42 patients].

Ann Chir Plast Esthet 2020 Apr 8;65(2):131-140. Epub 2020 Feb 8.

Centre des brûlés de Lyon Pierre-Colson, hôpital Edouard-Herriot, 5, place d'Arsonval, 69003 Lyon, France; Service de chirurgie plastique esthétique et reconstructrice, hôpital de la Croix-Rousse, 103, grande rue de la Croix-Rousse, 69004 Lyon, France.

Purpose: With constraints and a risk of complication, tissue expansion in child's burn sequelae need a controlled surgical procedure, and a therapeutic plan appropriate to the specific pediatric healing, growth, and development.

Material And Methods: Our principles of management and technical points are described. A retrospective study of tissue expansion in child's burn sequelae between 2005 and 2016 is submitted. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.anplas.2020.01.001DOI Listing

Tumescent Injections in Subcutaneous Pig Tissue Disperse Fluids Volumetrically and Maintain Elevated Local Concentrations of Additives for Several Hours, Suggesting a Treatment for Drug Resistant Wounds.

Pharm Res 2020 Feb 10;37(3):51. Epub 2020 Feb 10.

Department of Physics and Astronomy, University of California Los Angeles, California, Los Angeles, 90095, USA.

Purpose: Bolus injection of fluid into subcutaneous tissue results in accumulation of fluid at the injection site. The fluid does not form a pool. Rather, the injection pressure forces the interstitial matrix to expand to accommodate the excess fluid in its volume, and the fluid becomes bound similar to that in a hydrogel. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11095-020-2769-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010616PMC
February 2020

Single-cell transcriptome analysis of human skin identifies novel fibroblast subpopulation and enrichment of immune subsets in atopic dermatitis.

J Allergy Clin Immunol 2020 Feb 7. Epub 2020 Feb 7.

Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY. Electronic address:

Background: Atopic dermatitis (AD) is a prevalent inflammatory skin disease with a complex pathogenesis involving immune cell and epidermal abnormalities. Despite whole tissue biopsy studies that have advanced the mechanistic understanding of AD, single cell-based molecular alterations are largely unknown.

Objective: Our aims were to construct a detailed, high-resolution atlas of cell populations and assess variability in cell composition and cell-specific gene expression in the skin of patients with AD versus in controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaci.2020.01.042DOI Listing
February 2020

Regulated in Development and DNA Damage Responses 1 Prevents Dermal Adipocyte Differentiation and Is Required for Hair Cycle-Dependent Dermal Adipose Expansion.

J Invest Dermatol 2020 Feb 4. Epub 2020 Feb 4.

Department of Dermatology, Northwestern University, Chicago, Illinois, USA. Electronic address:

Dermal white adipose tissue (dWAT) expansion is associated with important homeostatic and pathologic processes in skin. Even though mTOR/protein kinase B signaling is important for adipogenesis, the role of regulated development of DNA damage responses 1 (REDD1), a negative regulator of mTOR/protein kinase B, is poorly understood. Loss of REDD1 in mice resulted in reduction of body mass, total fat, size of gonadal white adipose tissue, and interscapular brown adipose tissue. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jid.2019.12.033DOI Listing
February 2020

The role of the interleukin-23/Th17 pathway in cardiometabolic comorbidity associated with psoriasis.

J Eur Acad Dermatol Venereol 2020 Feb 5. Epub 2020 Feb 5.

Psoriasis-Center at the Department of Dermatology, University Medical Center Schleswig-Holstein, Kiel, Germany.

Alterations in the innate and adaptive immunity underpin psoriasis pathophysiology, with the Th17 cells subset now recognized as the fundamental cells in the key controlling pathway involved in its pathogenesis. Since psoriasis is a systemic disease with important comorbidity, further knowledge on the interleukin (IL)-23/Th17 axis led to the hypothesis that there may be shared pathogenic pathways between primary skin disease and comorbidity. Psoriasis has been identified as a risk factor for cardiovascular and metabolic disease, and increasing evidence gives support to this epidemiological observation from the clinical-pathologically field. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jdv.16273DOI Listing
February 2020

Cell-free fat extract promotes tissue regeneration in a tissue expansion model.

Stem Cell Res Ther 2020 Feb 4;11(1):50. Epub 2020 Feb 4.

Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, 639 ZhiZaoJu Road, Shanghai, 200011, China.

Background: Tissue expansion techniques play an important role in plastic surgery. How to improve the quality of the expanded skin and shorten the expansion period are still worth investigating. Our previous studies found that a cell-free fat extract (CEFFE) possessed pro-angiogenic and pro-proliferative activities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-020-1564-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001260PMC
February 2020
3.368 Impact Factor

Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates.

Nat Commun 2020 01 31;11(1):665. Epub 2020 Jan 31.

Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, 15213, USA.

Injury, surgery, and disease often disrupt tissues and it is the process of regeneration that aids the restoration of architecture and function. Regeneration can occur through multiple strategies including stem cell expansion, transdifferentiation, or proliferation of differentiated cells. We have identified a case of regeneration in Xenopus embryonic aggregates that restores a mucociliated epithelium from mesenchymal cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-14385-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994656PMC
January 2020

Early histological and ultrastructural changes in expanded murine scalp.

Ultrastruct Pathol 2020 Jan 28;44(1):141-152. Epub 2020 Jan 28.

Department of Plastic Surgery, Xijing Hospital; Air Force Medical University, Xi'an, Shaanxi Province, China.

Tissue expansion has been widely used for plastic, reconstructive, and esthetic surgeries. A mouse scalp expansion model can effectively mimic the characteristics of human skin expansion. However, a detailed study of the histological features and ultrastructural characteristics of expanded scalp is lacking, especially early ultrastructural changes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/01913123.2020.1720876DOI Listing
January 2020

Follow-Up Study: One-Step Salvage of Infected Prosthetic Breast Reconstructions Using Antibiotic-Impregnated Polymethylmethacrylate Plates and Concurrent Tissue Expander Exchange.

Plast Reconstr Surg 2020 Feb;145(2):240e-250e

Houston, Texas From the Division of Plastic Surgery, Baylor College of Medicine; and the Department of Plastic Surgery, Kelsey Seybold Clinic.

Background: Periprosthetic infections represent major complications in breast reconstruction, frequently leading to expander-implant loss. No consensus regarding a management algorithm for attempted salvage currently exists. This study assessed outcomes of the authors' salvage protocol using an antibiotic-impregnated polymethylmethacrylate implant with expander device exchange. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/PRS.0000000000006501DOI Listing
February 2020

The Broad Application of Prepectoral Direct-to-Implant Breast Reconstruction with Acellular Dermal Matrix Drape and Fluorescent Imaging in a Community Setting.

Plast Reconstr Surg 2020 Feb;145(2):291-300

Wilmington, N.C. From the Department of Surgery, New Hanover Regional Medical Center.

Background: Much has been written regarding the new paradigm of prepectoral direct-to-implant reconstruction, but patient selection continues to be unclear. Prepectoral direct-to-implant (PDTI) reconstruction with acellular dermal matrix drape and fluorescent imaging (ADFI) was offered to all patients.

Methods: The PDTI with ADFI protocol is (1) fluorescent imaging following mastectomy, (2) acellular dermal matrix prepectoral drape construction, (3) direct-to-implant placement beneath drape, and (4) repeated fluorescent imaging with implant in place. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/PRS.0000000000006496DOI Listing
February 2020

Predominance of Central Memory T Cells with High T-Cell Receptor Repertoire Diversity is Associated with Response to PD-1/PD-L1 Inhibition in Merkel Cell Carcinoma.

Clin Cancer Res 2020 May 13;26(9):2257-2267. Epub 2020 Jan 13.

Translational Skin Cancer Research, German Consortium for Translational Cancer Research (Deutsches Konsortium für Translationale Krebsforschung; DKTK), Essen, Germany.

Purpose: Merkel cell carcinoma (MCC) is an aggressive neuroendocrine skin cancer, which can be effectively controlled by immunotherapy with PD-1/PD-L1 checkpoint inhibitors. However, a significant proportion of patients are characterized by primary therapy resistance. Predictive biomarkers for response to immunotherapy are lacking. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-19-2244DOI Listing
May 2020
8.722 Impact Factor

Relationship between shape of peripheral initial lymphatics and efficiency of mechanical stimulation-induced lymph formation.

Microcirculation 2020 Jan 13. Epub 2020 Jan 13.

National Defense Medical College Research Institute, Tokorozawa, Japan.

Objective: The present study aimed to combine the physiological significance of irregularly shaped initial lymphatics and mechanisms of mechanical stimulation-induced lymph formation.

Methods: To confirm stretch-induced expansion of initial lymphatics, a finite element model that simulated morphological changes on a computer and fluorescent image and immunohistochemical analyses in mouse skin were adopted. Next, to quantitatively analyze the stretch-induced expansion, a simulation study was performed using a viscoelastic model of the tissue including initial lymphatics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/micc.12606DOI Listing
January 2020

Cultured Epidermal Autograft for Total Scalp Reconstruction in a Neonate Following Necrotizing Fasciitis.

Ann Plast Surg 2020 Jan 8. Epub 2020 Jan 8.

Division of Pediatric Plastic Surgery, Children's Memorial Hermann Hospital, Houston, TX.

Necrotizing fasciitis in the pediatric population is a particularly difficult diagnostic and management challenge. Options for soft tissue reconstruction of wounds following surgical debridement have been historically limited, yet recent advancements in bioengineered tissue and matrices have introduced alternative methods of treatment for these patients. We present a case of neonatal necrotizing fasciitis of the scalp requiring full-thickness surgical debridement, which was successfully reconstructed using Epicel cultured epidermal autograft (CEA). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/SAP.0000000000002235DOI Listing
January 2020

Serially expanded flap use to treat large hairless scalp lesions.

Arch Craniofac Surg 2019 Dec 20;20(6):408-411. Epub 2019 Dec 20.

Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, Seoul, Korea.

Hairless scalp areas can occur due to trauma, tumors, or congenital disease. This aesthetically unpleasing condition can lead to psychosocial distress, and thin skin flaps may be prone to scarring. Treating the hairless scalp by simple excision is challenging because of skin tension. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.7181/acfs.2019.00500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949505PMC
December 2019

Reconstruction of Large Ptotic Breasts After Nipple-Sparing Mastectomy: A Modified Buttonhole Technique.

Ann Plast Surg 2020 Jan 7. Epub 2020 Jan 7.

Department of Plastic and Reconstructive Surgery, Holy Redeemer Hospital, Meadowbrook, PA.

As nipple-sparing mastectomies continue to gain popularity, the reconstruction of a large ptotic breast remains a challenge. The purpose of this article is to introduce the senior author's modifications of a novel technique previously described for addressing the redundant skin envelope in ptotic breasts during immediate reconstruction after nipple-sparing mastectomy. In this review, 15 patients (29 breasts) were reconstructed using our modified "buttonhole" technique. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/SAP.0000000000002161DOI Listing
January 2020

Regeneration and expansion of autologous full-thickness skin through a self-propagating autologous skin graft technology.

Clin Case Rep 2019 Dec 6;7(12):2449-2455. Epub 2019 Nov 6.

Division of Plastic and Reconstructive Surgery Department of Surgery Louisiana State University Health Sciences Center New Orleans Louisiana.

New autologous skin regeneration technology yielded full-thickness skin as evidenced by clinical observation and skin biopsy 5 months after surgery, providing relief for debilitating split-thickness skin graft contracture in a pediatric burn case. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/ccr3.2533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935643PMC
December 2019

[Clinical effects of expanded forehead flaps in repairing midfacial defects].

Zhonghua Shao Shang Za Zhi 2019 Dec;35(12):855-858

Institute of Plastic Surgery of PLA, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.

To explore the clinical effects of expanded forehead flaps in repairing midfacial defects. From January 2003 to December 2018, 19 patients with midfacial defects were admitted to our unit, including 8 males and 11 females, aged 7 to 52 years. One cylindrical expander with rated capacity ranged from 100 to 170 mL was placed in the forehead of patients in the first stage of expansion, and the total water injection volume was about 2 times of the rated capacity of the expander during 1 to 2 months. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.issn.1009-2587.2019.12.005DOI Listing
December 2019

Innate lymphoid cells. New players in tissue homeostasis and inflammatory responses.

Medicina (B Aires) 2019 ;79(Spec 6/1):564-569

Laboratorio de Fisiopatología de la Inmunidad Innata, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Argentina.

In recent times, our understanding of the role of the immune system in different physiopathological situations has increased markedly. A new set of cells, generically known as innate lymphoid cells (ILC), has been discovered in the lymphoid compartment. Five ILC subsets can be recognized according to phenotypic and functional similarities with different subpopulations of T lymphocytes. Read More

View Article

Download full-text PDF

Source
February 2020

Pre-Expanded Latissimus Dorsi Myocutaneous Flap for Total Scalp Defect Reconstruction.

J Craniofac Surg 2020 Mar/Apr;31(2):e151-e153

Department of Plastic and Burn Surgery, West China Hospital of Sichuan University, Chengdu.

Scalp defects can be caused by various factors, and reconstruction options for scalp defects include skin grafts, local flaps, tissue expanders, and free flaps. However, currently, it is widely accepted that the use of free flaps is the most feasible method for extensive scalp defect reconstruction. While multiple flaps have been used to reconstruct scalp defects, the reconstruction of total scalp defects still remains challenging. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/SCS.0000000000006084DOI Listing
December 2019

Pemphigus vulgaris possibly associated with application of a tissue expander in a patient with Crohn's disease and primary sclerosing cholangitis.

Acta Dermatovenerol Alp Pannonica Adriat 2019 Dec;28(4):173-176

Center for Dermatologic Diseases, Limassol, Cyprus.

Pemphigus vulgaris (PV) is an autoimmune disease of the skin and mucous membranes characterized by suprabasal acantholysis and formation of blisters and erosions due to generation of IgG autoantibodies directed against desmosomal proteins. Tissue expanders are devices that, through controlled mechanical overstretch, are capable of generating new skin that is used to cover wounds or extended surgical defects. We report the case of a 13-year-old girl suffering from Crohn's disease (CD) and primary sclerosing cholangitis (PSC) who developed PV after application of a tissue expander for surgical removal of a giant congenital melanocytic nevus (GCMN). Read More

View Article

Download full-text PDF

Source
December 2019

Cell Intrinsic Deregulated ß-Catenin Signaling Promotes Expansion of Bone Marrow Derived Connective Tissue Type Mast Cells, Systemic Inflammation, and Colon Cancer.

Front Immunol 2019 3;10:2777. Epub 2019 Dec 3.

Department of Immunology, Mayo Clinic, Rochester, MN, United States.

Mast cells constitutively express ß-catenin and expand in solid tumors such as colon and skin cancer. However, the role of ß-catenin signaling in mast cells and the cause or effect of mast cell expansion and tumor growth has yet to be established. In earlier studies we used mast cell depletion and protease staining approaches, to provide evidence for a causative role of mast cells in small bowel polyposis, and related specific phenotypes and distributions of tumor infiltrating mast cells to stages of tumor growth. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2019.02777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902090PMC
December 2019

Loss of LRP1 promotes acquisition of contractile-myofibroblast phenotype and release of active TGF-β1 from ECM stores.

Matrix Biol 2020 Jun 11;88:69-88. Epub 2019 Dec 11.

Departments of Biochemistry and Internal Medicine, Universities of Giessen and Marburg Lung Center, Giessen, Germany. Electronic address:

In healing tissue, fibroblasts differentiate to α-smooth muscle actin (SMA)-expressing contractile-myofibroblasts, which pull the wound edges together ensuring proper tissue repair. Uncontrolled expansion of the myofibroblast population may, however, lead to excessive tissue scarring and finally to organ dysfunction. Here, we demonstrate that the loss of low-density lipoprotein receptor-related protein (LRP) 1 overactivates the JNK1/2-c-Jun-Fra-2 signaling pathway leading to the induction of α-SMA and periostin expression in human lung fibroblasts (hLF). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.matbio.2019.12.001DOI Listing
June 2020
5.074 Impact Factor

Correlations Between Interleukin-11 Expression and Hypertensive Kidney Injury in a Rat Model of Renovascular Hypertension.

Am J Hypertens 2020 Apr;33(4):331-340

Department of Nephrology and Hypertension, University Hospital of Erlangen, Erlangen, Germany.

Background: Interleukin-11 (IL-11) is a pleiotropic cytokine of the interleukin-6 family. Recent studies revealed its crucial role in the development of cardiovascular fibrosis. In this study we examined IL-11 expression levels in the heart and the kidney exposed to high blood pressure in renovascular hypertensive rats and their correlations to fibrotic markers and kidney injury. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/ajh/hpz194DOI Listing

Erbium fractional laser irradiation combined with autologous platelet-rich plasma and platelet-poor plasma application for facial rejuvenation.

J Cosmet Dermatol 2019 Dec 13. Epub 2019 Dec 13.

Department of Plastic Surgery, General Hospital of Southern Theater Command, PLA, Guangzhou, China.

Background: Erbium fractional laser treatment has immense skin rejuvenation effects, but it is associated with side effects such as erythema and pigmentation. Platelet-rich plasma (PRP) can enhance the restorative effects of Erbium fractional laser, but there are few studies on this combined treatment in the Asian population, and no study has examined the effects of adding platelet-poor plasma (PPP) to the combination.

Objective: This study aims to investigate the effects and safety of Erbium fractional laser irradiation combined with autologous PRP and PPP therapy for facial rejuvenation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jocd.13241DOI Listing
December 2019
5 Reads

In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics.

Sci Rep 2019 12 6;9(1):18561. Epub 2019 Dec 6.

School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University, Bentley, WA, 6102, Australia.

The long-term expansion of keratinocytes under conditions that avoid xenogeneic components (i.e. animal serum- and feeder cell-free) generally causes diminished proliferation and increased terminal differentiation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-54793-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897920PMC
December 2019
5.078 Impact Factor

Targeted Expansion of Tissue-Resident CD8 T Cells to Boost Cellular Immunity in the Skin.

Cell Rep 2019 12;29(10):2990-2997.e2

Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR 97239, USA; Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, OR 97239, USA; Department of Radiation Medicine, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address:

Tissue-resident memory (T) CD8 T cells are positioned within environmental barrier tissues to provide a first line of defense against pathogen entry, but whether these specialized T cell populations can be readily boosted to increase protective immunity is ill defined. Here, we demonstrate that repeated activation of rare, endogenous T CD8 T cells, using only topical application of antigenic peptide causes delayed-type hypersensitivity and increases the number of antigen-specific T CD8 T cells, specifically in the challenged skin by ∼15-fold. Expanded T CD8 T cells in the skin are derived from memory T cells recruited out of the circulation that became CD69 tissue residents following a local antigen encounter. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2019.10.126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914228PMC
December 2019

Implant-sparing Mastectomy: An Alternative for Women Undergoing Mastectomy With Retropectoral Implants.

Clin Breast Cancer 2020 Feb 3;20(1):e14-e19. Epub 2019 Sep 3.

Breast Department, H. Lee Moffitt Cancer Center, Tampa, FL. Electronic address:

Introduction: Implant-sparing mastectomy (ISM) is a skin-sparing mastectomy that preserves a retropectoral implant and potentially eliminates the need for tissue expansion or complex reconstruction. This study aimed to determine oncologic and surgical outcomes and reconstructive patterns in patients undergoing ISM.

Patients And Methods: A single-institution, retrospective review of patients undergoing ISM from 2006 to 2018 was performed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clbc.2019.08.007DOI Listing
February 2020