2,730 results match your criteria Skin Tissue Expansion


Correction of Tanzer Type IIB Constricted Ears via Z-shaped Double V-Y Advancement Flaps.

Ann Plast Surg 2019 Jan 9. Epub 2019 Jan 9.

From the Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: Correction of Tanzer type IIB constricted ears requires sufficient expansion of the auricular skin to cover the reconstructed cartilaginous framework. To obtain an adequately sized skin cover, skin flap transposition from periauricular donor sites has been described; however, it remains challenging to obtain enough skin to enable satisfactory reconstruction. Herein, we describe a novel method that uses 2 local flaps comprising superoauricular, retroauricular, and preauricular soft tissue to expand the skin cover and correct Tanzer type IIB deformity. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001750DOI Listing
January 2019

Transplantation of Adipose-Derived Stem Cells Before Flap Expansion and After Expanded Flap Elevation Result in Different Effects.

Ann Plast Surg 2019 Feb;82(2):237-244

Columbia University Mailman School of Public Health, New York, NY.

Background: Studies of using mesenchymal stem cells to assist skin and soft tissue expansion have shown that stem cells can improve expansion efficiency through promoting tissue regeneration. However, the issue that whether the flap viability is also improved is unknown.

Methods: Sixteen pigs were equally divided into 2 groups. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001736DOI Listing
February 2019

Quantitative assessment and risk factors for chest wall deformity resulting from tissue expansion for breast reconstruction.

Breast Cancer 2019 Jan 2. Epub 2019 Jan 2.

Department of Oral and Maxillofacial Surgery, and Plastic Surgery, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma, 371-8511, Japan.

Background: Chest wall deformity after tissue expansion for breast reconstruction is less recognized than complications such as infection, hematoma, skin necrosis and capsular contracture. However, the condition should not be discounted because pain, rib fracture and dyspnea may occur in severe cases. The aim of this study is to evaluate the extent of chest wall deformity quantitatively using computed tomography (CT) and to identify risk factors for this condition after tissue expansion. Read More

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http://dx.doi.org/10.1007/s12282-018-00942-3DOI Listing
January 2019

Full Thickness Skin Expansion in a Newly Developed Reactor and Evaluation of Auto-Grafting Efficiency of the Expanded Skin Using Yucatan Pig Model.

Tissue Eng Regen Med 2018 Oct 1;15(5):629-638. Epub 2018 Sep 1.

Biomedical Research Institute, Joint Institute for Regenerative Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, 41944 Republic of Korea.

Background: Skin grafts are required in numerous clinical procedures, such as reconstruction after skin removal and correction of contracture or scarring after severe skin loss caused by burns, accidents, and trauma. The current standard for skin defect replacement procedures is the use of autologous skin grafts. However, donor-site tissue availability remains a major obstacle for the successful replacement of skin defects and often limits this option. Read More

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http://dx.doi.org/10.1007/s13770-018-0154-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171704PMC
October 2018

Staged reconstructive treatment for extensive irregular cicatricial alopecia after burn.

Medicine (Baltimore) 2018 Dec;97(52):e13522

Department of Plastic Surgery, Affiliated Hangzhou First People's Hospital.

For extensive irregular cicatricial alopecia after burn, effective and pleasing restoration of hair-bearing scalp remains challenging. In this article, the authors presented staged reconstructive treatment for extensive irregular cicatricial alopecia with the goal to achieve better and reliable results. A retrospective review of staged reconstructive treatment performed in 16 patients with extensive irregular cicatricial alopecia after burn was conducted. Read More

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http://Insights.ovid.com/crossref?an=00005792-201812280-0000
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http://dx.doi.org/10.1097/MD.0000000000013522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314779PMC
December 2018
2 Reads

Advances in diagnosis of mastocytosis and hypereosinophilic syndrome.

Semin Hematol 2019 Jan 28;56(1):22-29. Epub 2018 May 28.

Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508.

Mastocytosis and hypereosinophilic syndrome is very rare neoplastic hematopoietic diseases. Mastocytosis is characterized by expansion and accumulation of clonal tissue mast cells in skin and/or various internal organs, while hypereosinophilic syndrome manifests with an increased number of eosinophils in the peripheral blood and tissue damage. These diseases represent a diagnostic challenge, since they can have overlapping clinical and pathologic features. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00371963183003
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http://dx.doi.org/10.1053/j.seminhematol.2018.05.005DOI Listing
January 2019
5 Reads

Use of Tissue Expander for Contracted Scarred Saddle Deformity Rhinoplasty.

Facial Plast Surg 2018 Dec 19. Epub 2018 Dec 19.

Division of Facial Plastic Surgery, Department of Otolaryngology, Loma Linda University Health, Loma Linda, California.

The main purpose of this article is to present an alternative technique for the reconstruction of saddle nose deformity in patients with severely scarred or contracted soft tissue envelopes. In this single surgeon case series, the authors present a two-staged reconstruction performed on four adult patients with saddle nose deformities and contracted soft tissue envelope stemming from a variety of etiologic factors including vasculitis, sarcoidosis, and trauma. Stage one involved placement of a 1 × 4 cm tissue expander along the nasal dorsum through anterior scalp and endonasal incisions. Read More

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http://dx.doi.org/10.1055/s-0038-1675632DOI Listing
December 2018

"Delivery of External Volume Expansion (EVE) through Micro-deformational Interfaces Safely Induces Angiogenesis in a Murine Model of Intact Diabetic Skin with Endothelial Cell Dysfunction (ECD)".

Plast Reconstr Surg 2018 Nov 26. Epub 2018 Nov 26.

Tissue Engineering and Wound Healing Laboratory, Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital-Harvard Medical School, Boston, USA.

Introduction: External Volume Expansion (EVE) promotes angiogenesis in tissues by inducing a sub-critical hypoxia, mechanically stimulating endothelia, and creating mild inflammation. Application of EVE to less-vascularized tissues with endothelial cell dysfunction (ECD) (e.g. Read More

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http://dx.doi.org/10.1097/PRS.0000000000005267DOI Listing
November 2018

Treatment With Topical Deferoxamine Improves Cutaneous Vascularity and Tissue Pliability in an Irradiated Animal Model of Tissue Expander-Based Breast Reconstruction.

Ann Plast Surg 2019 Jan;82(1):104-109

Division of Plastic Surgery, University of Kentucky, Lexington, KY.

Purpose: Postmastectomy radiation therapy is an important component of the multimodality approach to later-stage breast cancers. Unfortunately, despite its proven survival benefits, postmastectomy radiation therapy is deleterious to the skin and soft tissue, causing increased complications and worse aesthetic outcomes after breast reconstruction.There is currently no effective pharmaceutical agent to mitigate the soft tissue fibrosis and hypovascularity associated with soft tissue radiation. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001655DOI Listing
January 2019

Immediate breast reconstruction in high-risk cases using an anatomically shaped permanent expandable implant.

J Plast Reconstr Aesthet Surg 2018 Nov 13. Epub 2018 Nov 13.

Plastic and Reconstructive Breast Surgery Unit, Department of Plastic Surgery, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, 6 Weizmann St., Tel-Aviv 6423906, Israel. Electronic address:

Background: Anatomically shaped permanent expandable implants are dual-chambered devices, which allow their postoperative expansion for the adjustment of volume. Their use is optional when 1-stage immediate breast reconstruction (IBR) direct to permanent implant is not feasible. The aim of this study is to present one center's long-term experience with the anatomical Becker expandable implant in a large series of patients who underwent either IBR or a salvage procedure for IBR with the device. Read More

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http://dx.doi.org/10.1016/j.bjps.2018.10.030DOI Listing
November 2018

Experiences with a temporary synthetic skin substitute after decompressive craniectomy: a retrospective two-center analysis.

Acta Neurochir (Wien) 2018 Dec 4. Epub 2018 Dec 4.

Department of Neurosurgery, Trauma Center Murnau, Professor-Küntscher Straße 8, 82418, Murnau, Germany.

Background: Decompressive craniectomy is a commonly performed procedure. It reduces intracranial pressure, improves survival, and thus might have a positive impact on several neurosurgical diseases and emergencies. Sometimes primary skin closure is not possible due to cerebral herniation or extensive skin defects. Read More

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http://dx.doi.org/10.1007/s00701-018-3748-9DOI Listing
December 2018
2 Reads

Assisting Rapid Soft-Tissue Expansion with Adipose-Derived Stem Cells: An Experimental Study in a Pig Model.

Plast Reconstr Surg 2018 Nov;142(5):674e-684e

Beijing, People's Republic of China From the Head & Neck Aesthetic and Plastic Surgery Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Background: Rapid tissue expansion has been attempted, aiming at shortening the period of conventional expansion. However, it has scarcely been clinically applied because of its drawbacks such as low expansion efficiency and tissue destruction. Adipose-derived stem cell transplantation is a promising therapeutic method in regenerative medicine. Read More

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http://dx.doi.org/10.1097/PRS.0000000000004884DOI Listing
November 2018
4 Reads

Microtia Reconstruction in Hemifacial Microsomia Patients: Three Framework Coverage Techniques.

Plast Reconstr Surg 2018 Dec;142(6):1558-1570

Seoul, Republic of Korea From the Seoul Center for Developmental Ear Anomalies and the Department of Plastic and Reconstructive Surgery, Korea University Anam Hospital.

Background: Microtia with hemifacial microsomia is difficult to treat because of skin volume deficiency. To provide further information for coverage techniques in microtia reconstruction, the authors have reviewed and analyzed patients who underwent surgery at their center.

Methods: A total 52 patients with microtia with hemifacial microsomia who underwent reconstruction between 2006 and 2016 were involved. Read More

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http://dx.doi.org/10.1097/PRS.0000000000005063DOI Listing
December 2018
1 Read

Reconstruction of a surgical defect in the popliteal fossa: A case report.

Int J Surg Case Rep 2018 1;53:228-230. Epub 2018 Nov 1.

Columbia University Medical Center, Department of Dermatology, New York, NY, 10032, United States. Electronic address:

Introduction: Surgical defects of the popliteal fossa pose a reconstructive challenge to the surgeon, given the intimate relationship of this area with the knee joint and underlying vital neuro-vasculature.

Case Presentation: An 88-year-old woman was treated for a biopsy proven invasive squamous cell carcinoma of the right popliteal fossa with Mohs micrographic surgery. The resultant defect (4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22102612183049
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http://dx.doi.org/10.1016/j.ijscr.2018.10.070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232581PMC
November 2018
13 Reads

Reconstruction of the Forehead and Scalp.

Facial Plast Surg Clin North Am 2019 Feb;27(1):85-94

Department of Otolaryngology-Head and Neck Surgery, New York University School of Medicine, 240 East 38th Street, 14th Floor, New York, NY 10016, USA. Electronic address:

Tissue inelasticity and the hair-bearing nature of the scalp and forehead pose unique challenges during reconstruction. A thorough understanding of the surgical anatomy of the scalp and forehead is paramount for optimal reconstructive outcomes. Primary wound closure is usually preferred over secondary intention healing and skin grafting. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10647406183007
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http://dx.doi.org/10.1016/j.fsc.2018.08.009DOI Listing
February 2019
12 Reads

The proliferation and clonal migration of B cells in the systemic and mucosal tissues of channel catfish suggests there is an interconnected mucosal immune system.

Fish Shellfish Immunol 2019 Jan 7;84:1134-1144. Epub 2018 Nov 7.

Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216-4505, USA.

IgM transcripts from different mucosal and systemic tissues from a single adult channel catfish have been evaluated. Arrayed heavy chain cDNA libraries from each of these different mucosal and systemic tissues were separately constructed, hybridized with VH family specific probes and a variety of approaches were used to define their structural relationships. Baseline hybridization studies indicated that the tissue libraries had different VH expression patterns, and sequencing studies indicated this was not simply due to varying proportions of the same B cell population. Read More

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http://dx.doi.org/10.1016/j.fsi.2018.11.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335153PMC
January 2019
3 Reads

Additive effect of bFGF and selenium on expansion and paracrine action of human amniotic fluid-derived mesenchymal stem cells.

Stem Cell Res Ther 2018 Nov 8;9(1):293. Epub 2018 Nov 8.

Laboratory of Cell Function Regulation, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-go, Seoul, 02841, Republic of Korea.

Background: Mesenchymal stem cell-derived conditioned medium (MSC-CM) has emerged as a promising cell-free tool for restoring degenerative diseases and treating traumatic injuries. The present study describes the effect of selenium as a reactive oxygen species (ROS) scavenger and its additive effect with basic fibroblast growth factor (bFGF) on in vitro expansion of amniotic fluid (AF)-MSCs and the paracrine actions of AF-MSC-CM as well as the associated cellular and molecular mechanisms.

Methods: In this study, we obtained CM from human AF-MSCs cultured with selenium. Read More

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http://dx.doi.org/10.1186/s13287-018-1058-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225588PMC
November 2018

Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin.

JCI Insight 2018 Nov 2;3(21). Epub 2018 Nov 2.

Department of Dermatology, UCSD, La Jolla, California, USA.

In this study we evaluated the role of hyaluronan (HA) in reactive adipogenesis, a local expansion of preadipocytes that provides host defense by release of antimicrobial peptides. We observed that HA accumulated during maturation of adipocytes in vitro and was associated with increased expression of preadipocyte factor 1, zinc finger protein 423, and early B cell factor 1. Although HA is normally abundant in the extracellular matrix, a further increase in HA staining occurred in mice at sites of reactive adipogenesis following injury of colon by dextran sodium sulfate or injury of skin from infection with Staphylococcus aureus. Read More

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http://dx.doi.org/10.1172/jci.insight.123072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238730PMC
November 2018

Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes.

Pediatr Surg Int 2019 Jan 31;35(1):121-127. Epub 2018 Oct 31.

Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032, Zurich, Switzerland.

Aim Of The Study: The use of autologous bio-engineered dermo-epidermal skin substitutes (DESS) yields a pivotal opportunity to cover large skin defects in human patients. These skin grafts consist of both epidermal and dermal compartments necessary for robust and permanent functional wound closure. In this study, we investigated the impact of mesenchymal cells derived from different body site origins on the expression pattern of diverse markers within DESS. Read More

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http://dx.doi.org/10.1007/s00383-018-4383-5DOI Listing
January 2019

Oncogenic activation of PI3K induces progenitor cell differentiation to suppress epidermal growth.

Nat Cell Biol 2018 Nov 22;20(11):1256-1266. Epub 2018 Oct 22.

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Oncogenic lesions are surprisingly common in morphologically and functionally normal human skin. However, the cellular and molecular mechanisms that suppress their cancer-driving potential to maintain tissue homeostasis are unknown. By employing assays for the direct and quantitative assessment of cell fate choices in vivo, we show that oncogenic activation of PI3K-AKT, the most commonly activated oncogenic pathway in cancer, promotes the differentiation and cell cycle exit of epidermal progenitors. Read More

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http://dx.doi.org/10.1038/s41556-018-0218-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291208PMC
November 2018

High-Salt Diet Causes Expansion of the Lymphatic Network and Increased Lymph Flow in Skin and Muscle of Rats.

Arterioscler Thromb Vasc Biol 2018 Sep;38(9):2054-2064

From the Department of Biomedicine, University of Bergen, Norway (T.V.K., E.N., J.H., T.R., O.T., H.W.).

Objective- A commonly accepted pivotal mechanism in fluid volume and blood pressure regulation is the parallel relationship between body Na and extracellular fluid content. Several recent studies have, however, shown that a considerable amount of Na can be retained in skin without commensurate water retention. Here, we asked whether a salt accumulation shown to result in VEGF (vascular endothelial growth factor)-C secretion and lymphangiogenesis had any influence on lymphatic function. Read More

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http://dx.doi.org/10.1161/ATVBAHA.118.311149DOI Listing
September 2018
7 Reads

Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling.

Cell Rep 2018 Oct;25(3):598-610.e5

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63108, USA; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108, USA. Electronic address:

Despite substantial self-renewal capability in vivo, epithelial stem and progenitor cells located in various tissues expand for a few passages in vitro in feeder-free condition before they succumb to growth arrest. Here, we describe the EpiX method, which utilizes small molecules that inhibit PAK1-ROCK-Myosin II and TGF-β signaling to achieve over one trillion-fold expansion of human epithelial stem and progenitor cells from skin, airway, mammary, and prostate glands in the absence of feeder cells. Transcriptomic and epigenomic studies show that this condition helps epithelial cells to overcome stresses for continuous proliferation. Read More

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http://dx.doi.org/10.1016/j.celrep.2018.09.072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6284236PMC
October 2018
3 Reads

New economical and simple device for intraoperative expansion on small and medium sized soft tissue defects.

Arch Craniofac Surg 2018 Sep 20;19(3):235-239. Epub 2018 Sep 20.

Department of Plastic and Reconstructive Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.

Intraoperative expansion has been used to cover small to large defects without disadvantages of the conventional tissue expanders. Various materials, for example, expanders and Foley catheters are being used. We introduce a new, convenient and economical device immediately available in the operating room, according to the defect size for intraoperative expansion, with latex gloves or balloons. Read More

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http://e-acfs.org/journal/view.php?doi=10.7181/acfs.2018.019
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http://dx.doi.org/10.7181/acfs.2018.01998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177679PMC
September 2018
7 Reads

A novel technique for large and ptotic breast reconstruction using a latissimus dorsi myocutaneous flap set at the posterior aspect, combined with a silicone implant, following tissue expander surgery.

Arch Plast Surg 2018 Sep 15;45(5):484-489. Epub 2018 Sep 15.

Department of Plastic and Reconstructive Surgery, Keio University, Tokyo, Japan.

Large and ptotic breast reconstruction in patients who are not candidates for a transverse rectus abdominalis myocutaneous flap and revision surgery for the contralateral breast remains challenging. We developed a novel breast reconstruction technique using a latissimus dorsi myocutaneous (LD m-c) flap set at the posterior aspect of the reconstructed breast, combined with an anatomical silicone breast implant (SBI), following tissue expander surgery. We performed the proposed technique in four patients, in whom the weight of the resected tissue during mastectomy was >500 g and the depth of the inframammary fold (IMF) was >3 cm. Read More

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http://e-aps.org/journal/view.php?doi=10.5999/aps.2017.01781
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http://dx.doi.org/10.5999/aps.2017.01781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177628PMC
September 2018
1 Read

Mid-axillary pacemaker re-implantation after contralateral pocket infection in an emaciated elderly case.

J Cardiol Cases 2018 Aug 9;18(2):70-73. Epub 2018 Jun 9.

Department of Cardiovascular Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.

The number of implantations of cardiac implantable electrophysiological devices (CIEDs) has increased over the past several years. However, the aging population and expansion of indications for CIEDs have led to an increase in associated infections. We experienced a case of a 99-year-old man presenting with skin erosion at the pocket site, where a 6-month-old implantable pacemaker was replaced. Read More

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http://dx.doi.org/10.1016/j.jccase.2018.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149616PMC
August 2018
2 Reads

Changes in the Biomechanical Properties of Human Skin in Hyperthermic and Hypothermic Ranges.

Wounds 2018 Sep;30(9):257-262

Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tuebingen, Germany.

Introduction: The prevalence of thermal skin injuries is high. Despite new research findings, skin burns and acute cold-contact injuries, together with resulting tissue damage, are not entirely understood. In particular, little is known about how these types of injuries alter the biomechanical properties of skin. Read More

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September 2018
14 Reads

Body Wall Reconstruction for Conjoined Twins: Our Experience and Lessons Learned.

Ann Plast Surg 2018 Dec;81(6S Suppl 1):S66-S70

From the Department of Plastic Surgery, Union Hospital, and.

Background: Conjoined twinning is a rare congenital malformation, and the resultant huge body wall defects after separation of conjoined twins represent a real challenge to surgeons.

Methods: From 2004 to 2009, authors performed body wall reconstruction for 2 pairs of thoraco-omphalopagus twins and 1 pair of ischiopagus tetrapus twins. Before separation, the techniques of tissue expansion and tractive training were adopted to acquire sufficient skin for final coverage. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001609DOI Listing
December 2018
3 Reads

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope.

Eur Surg Res 2018 21;59(3-4):265-275. Epub 2018 Sep 21.

IZKF Research Group Tissue Regeneration in Musculoskeletal Diseases, University Clinics Würzburg, Würzburg,

Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Read More

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https://www.karger.com/Article/FullText/492415
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http://dx.doi.org/10.1159/000492415DOI Listing
September 2018
3 Reads

Fluid absorption by skin tissue during intradermal injections through hollow microneedles.

Sci Rep 2018 Sep 13;8(1):13749. Epub 2018 Sep 13.

Department of Mechanical Engineering, The University of British Columbia, 2054-6250 Applied Science Lane, Vancouver, British Columbia, V6T 1Z4, Canada.

Hollow microneedles are an emerging technology for delivering drugs and therapeutics, such as vaccines and insulin, into the skin. Although the benefits of intradermal drug delivery have been known for decades, our understanding of fluid absorption by skin tissue has been limited due to the difficulties in imaging a highly scattering biological material such as skin. Here, we report the first real-time imaging of skin tissue at the microscale during intradermal injections through hollow microneedles, using optical coherence tomography. Read More

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http://dx.doi.org/10.1038/s41598-018-32026-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137045PMC
September 2018

Large Scalp Defect Repair with Flap Reconstruction Using Tissue Expander After Combined Bypass in Case of Moyamoya Disease.

World Neurosurg 2018 Dec 7;120:185-189. Epub 2018 Sep 7.

Department of Neurosurgery, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Bucheon-si, Gyeonggi-do, Republic of Korea. Electronic address:

Background: Combined revascularization is the preferred surgical management of adult Moyamoya disease. However, postoperative flap necrosis of the scalp is not an uncommon complication. We investigated the role of scalp incision design on the basis of the course of the superficial temporal artery (STA) to prevent postoperative scalp necrosis. Read More

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http://dx.doi.org/10.1016/j.wneu.2018.08.221DOI Listing
December 2018
6 Reads

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue.

J Vis Exp 2018 08 25(138). Epub 2018 Aug 25.

Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University; Suzhou Institute of Shandong University;

Primary human keratinocytes isolated from fresh skin tissues and their expansion in vitro have been widely used for laboratory research and for clinical applications. The conventional isolation method of human keratinocytes involves a two-step sequential enzymatic digestion procedure, which has been proven to be inefficient in generating primary cells from adult tissues due to the low cell recovery rate and reduced cell viability. We recently reported an advanced method to isolate human primary epidermal progenitor cells from skin tissues that utilizes the Rho kinase inhibitor Y-27632 in the medium. Read More

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https://www.jove.com/video/57784/a-simplified-efficient-meth
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http://dx.doi.org/10.3791/57784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231857PMC
August 2018
10 Reads

Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.

Nat Commun 2018 09 4;9(1):3592. Epub 2018 Sep 4.

Department of Molecular, Cellular and Developmental Biology, Yale University, 219 Prospect St., New Haven, CT, 06520, USA.

Adipocytes undergo pronounced changes in size and behavior to support diverse tissue functions, but the mechanisms that control these changes are not well understood. Mammary gland-associated white adipose tissue (mgWAT) regresses in support of milk fat production during lactation and expands during the subsequent involution of milk-producing epithelial cells, providing one of the most marked physiological examples of adipose growth. We examined cellular mechanisms and functional implications of adipocyte and lipid dynamics in the mouse mammary gland (MG). Read More

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http://dx.doi.org/10.1038/s41467-018-05911-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123393PMC
September 2018
1 Read

Advances in Wound Management.

J Am Acad Orthop Surg 2018 Dec;26(23):833-843

From the Department of Surgery, Albany Medical Center, Albany, NY.

Wound management is a notable healthcare and financial burden, accounting for >$10 billion in annual healthcare spending in the United States. A multidisciplinary approach involving orthopaedic and plastic surgeons, wound care nursing, and medical and support staff is often necessary to improve outcomes. Orthopaedic surgeons must be familiar with the fundamental principles and evidenced-based concepts for the management of acute and chronic wounds. Read More

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http://Insights.ovid.com/crossref?an=00124635-900000000-9960
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http://dx.doi.org/10.5435/JAAOS-D-17-00024DOI Listing
December 2018
7 Reads

Tissue Contraction-A New Paradigm in Breast Reconstruction.

Plast Reconstr Surg Glob Open 2018 Jul 13;6(7):e1865. Epub 2018 Jul 13.

Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Fla.

Background: Skin expansion is commonly needed in order to reconstruct the breast following modified radical mastectomy. With the advent of skin-sparing and nipple-sparing techniques, expansion is no longer necessary. The natural ability of healing tissues to contract can be advantageously used to improve the outcomes of immediate prepectoral breast reconstruction. Read More

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http://dx.doi.org/10.1097/GOX.0000000000001865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110695PMC

A Penetrating Facial Wound With Burn Injury.

J Craniofac Surg 2018 Oct;29(7):1900-1902

Department of Plastic & Reconstructive Surgery.

A patient presented with a complex penetrating facial wound by high temperature steel. The hot steel penetrated right temple, ethmoid bone, and maxillary sinus, and then exited from his left cheek. He kept his right eye but lost his sight. Read More

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http://dx.doi.org/10.1097/SCS.0000000000004923DOI Listing
October 2018
20 Reads

[New progress in the treatment of chronic wound of diabetic foot].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2018 Jul;32(7):832-837

Department of Orthopaedics, Beijing Shijitan Hospital, CMU, Beijing, 100038, P.R.China.

Diabetic foot is one of the serious complications of diabetic patients. It is caused by diabetes combined with different degrees of lower extremity vascular lesions and neuropathy, and the wound can not heal for a long time. The serious results can cause bone marrow infection, bone destruction, and have high disability and death rate. Read More

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http://www.rrsurg.com/article/10.7507/1002-1892.201806058
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http://dx.doi.org/10.7507/1002-1892.201806058DOI Listing
July 2018
16 Reads

Increment of growth factors in mouse skin treated with non-thermal plasma.

Int J Med Sci 2018 30;15(11):1203-1209. Epub 2018 Jul 30.

Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea.

Non-thermal plasma (NTP) has several beneficial effects, and can be applied as a novel instrument for skin treatment. Recently, many types of NTP have been developed for potential medical or clinical applications, but their direct effects on skin activation remain unclear. In this study, the effect of NTP on the alteration of mouse skin tissue was analyzed. Read More

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http://www.medsci.org/v15p1203.htm
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http://dx.doi.org/10.7150/ijms.26342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097260PMC
January 2019
18 Reads
2.003 Impact Factor

Remote Effects of Transplanted Perivascular Adipose Tissue on Endothelial Function and Atherosclerosis.

Cardiovasc Drugs Ther 2018 Oct;32(5):503-510

Department of Medicine, Cardiology Division, Vascular Biology Center, Medical College of Georgia at Augusta University, 1460 Laney Walker Blvd, Augusta, GA, 30912, USA.

Purpose: Perivascular adipose tissue (PVAT) surrounds the arterial adventitia and plays an important role in vascular homeostasis. PVAT expands in obesity, and inflamed PVAT can locally promote endothelial dysfunction and atherosclerosis. Here, using adipose tissue transplantation, we tested the hypothesis that expansion of PVAT can also remotely exacerbate vascular disease. Read More

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http://dx.doi.org/10.1007/s10557-018-6821-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292666PMC
October 2018
3 Reads
3.190 Impact Factor

Use of expanded deltopectoral skin flaps for facial reconstruction after sizeable benign tumor resections.

Am J Transl Res 2018 15;10(7):2158-2163. Epub 2018 Jul 15.

Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University Xi'an 710032, Shaanxi Province, China.

The overall unsightliness of expansive benign facial tumors imposes both physical and mental suffering. Although excision is generally the optimal recourse in such instances, reconstructing the subsequent surgical defects is always a critical issue. Herein, we have described our experiences using expanded deltopectoral skin flaps to manage large facial wounds after excising benign tumors. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079131PMC
July 2018
3.230 Impact Factor

Tissue expansion of the lower limb: Retrospective study of 141 procedures in burn sequelae.

Burns 2018 Nov 26;44(7):1851-1857. Epub 2018 Jul 26.

Plastic, Reconstructive and Cosmetic Unit, Burn Care Center, Hôpital Saint Louis, Paris, France.

Introduction: Skin expansion in the lower limb is a difficult procedure with a significant rate of complications. We propose, in this retrospective mono centric study, a systematic review of our skin expansion procedures on the lower limb in burn sequelae with a 30-years follow-up. The objective was to evaluate the overall result of our procedure and present its technical specificities. Read More

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http://dx.doi.org/10.1016/j.burns.2018.03.021DOI Listing
November 2018

Novel Insights into Adult and Cancer Stem Cell Biology.

Stem Cells Dev 2018 Nov 29;27(22):1527-1539. Epub 2018 Aug 29.

Stem Cell Biology Department, ICMR-National Institute for Research in Reproductive Health , Mumbai, India .

Adult tissues are thought to harbor two populations of "dormant" and "actively dividing" stem cells. Quiescent stem cells undergo rare asymmetric cell divisions (ACDs) through which they self-renew and give rise to tissue-committed "progenitors" of distinct fate and "progenitors" in turn undergo symmetric cell divisions (SCDs) and clonal expansion. However, quiescent stem cells have not been demonstrated in adult tissues such as skin, testis, liver, and brain. Read More

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http://dx.doi.org/10.1089/scd.2018.0118DOI Listing
November 2018
10 Reads
1 Citation
3.730 Impact Factor

[Breast reconstruction with temporary tissue expander].

Ann Chir Plast Esthet 2018 Nov 21;63(5-6):545-558. Epub 2018 Jul 21.

Service de chirurgie plastique, reconstructrice, et esthétique, hôpital civil, hôpitaux universitaires de Strasbourg, 1, place de l'Hôpital, 67000 Strasbourg, France.

Tissue expansion in breast reconstruction is a technique based on a simple principle but subjected to numerous traps. Its success relies on the patient selection, a good preparation of the skin pocket, no rush during the inflating time, and a specific care during the definitive implant placement inside the skin pocket. This article aims to list and detail point-by-point the different surgical timings, their practical application, the pitfalls and the possible traps to avoid, in order to succeed in breast reconstruction using tissue expander. Read More

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http://dx.doi.org/10.1016/j.anplas.2018.06.002DOI Listing
November 2018
1 Read

Capsular Biofilm Formation at the Interface of Textured Expanders and Human Acellular Dermal Matrix.

Plast Reconstr Surg 2018 10;142(4):587e

Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.

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http://Insights.ovid.com/crossref?an=00006534-201810000-0006
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http://dx.doi.org/10.1097/PRS.0000000000004781DOI Listing
October 2018
1 Read

Reply: Capsular Biofilm Formation at the Interface of Textured Implants and Acellular Dermal Matrix: A Comparative Scanning Electron Microscopy Study.

Plast Reconstr Surg 2018 10;142(4):587e-588e

Plastic and Reconstructive Surgery Department, University of Montreal Hospital Center, Montreal, Quebec, Canada.

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http://Insights.ovid.com/crossref?an=00006534-201810000-0006
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http://dx.doi.org/10.1097/PRS.0000000000004782DOI Listing
October 2018
6 Reads

Impact of Wnt signals on human intervertebral disc cell regeneration.

J Orthop Res 2018 Dec 6;36(12):3196-3207. Epub 2018 Aug 6.

Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown 26506-9196, West Virginia.

Although preconditioning strategies are growing areas of interest for therapies targeting intervertebral discs (IVDs), it is unknown whether the Wnt signals previously implicated in chondrogenesis, Wnt3A, Wnt5A, and Wnt11, play key roles in the promotion of human nucleus pulposus (NP) cell redifferentiation. In this study, NP cells isolated from herniated disc patients were transduced with lentiviral vectors to overexpress the WNT3A, WNT5A, or WNT11 genes, or CRISPR associated protein 9 (Cas9)/single-guide RNA (sgRNA) vectors to knock out these genes. Following expansion, transduced NP cells were induced for redifferentiation toward the NP phenotype. Read More

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http://dx.doi.org/10.1002/jor.24115DOI Listing
December 2018

Granulation tissue-derived mesenchymal stromal cells: a potential application for burn wound healing in pediatric patients.

J Stem Cells Regen Med 2018 30;14(1):53-58. Epub 2018 May 30.

Pediatric Unit, Department of Internal Medicine, University of Pavia and Department of Maternal and Children's Health, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Multipotential cells are mobilized into peripheral blood in response to trauma, in particular in severe burns. These cells migrate to the site of injury in response to chemotactic signals to modulate inflammation, repair damaged tissue and facilitate tissue regeneration. We evaluated the possibility of isolating and expand mesenchymal stromal cells (MSCs) from granulation tissue (GT) during debridement of a burn wound, as a persective strategy to improve skin regeneration. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6043658PMC

New Insights into Clonal Mast Cell Disorders Including Mastocytosis.

Immunol Allergy Clin North Am 2018 08 9;38(3):341-350. Epub 2018 Jun 9.

Division of Allergy and Clinical Immunology, Department of Internal Medicine, University of Michigan, 24 Frank Lloyd Wright Drive, PO Box 442, Suite H-2100, Ann Arbor, MI 48106-0442, USA. Electronic address:

Mastocytosis is a heterogeneous group of neoplasms that involve the clonal expansion of mast cells into one or more organ systems, which typically involves the skin and hematopoietic systems. Systemic mastocytosis consists of a multifocal infiltration of mast cells into various noncutaneous tissue sites, especially the bone marrow. Diagnosis requires tissue confirmation, and algorithms have been developed to assist clinicians in this process. Read More

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http://dx.doi.org/10.1016/j.iac.2018.04.014DOI Listing
August 2018
7 Reads

Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model.

Sci Rep 2018 Jul 3;8(1):10044. Epub 2018 Jul 3.

Radboud university medical center, department of Laboratory Medicine-Medical Immunology, Nijmegen, The Netherlands.

Recent regulatory T cell (Treg) based clinical trials support their therapeutic potential in transplantation and auto-inflammatory diseases. However, large numbers of Treg are needed to accomplish therapeutic efficacy. Local injection at the site of inflammation (targeted delivery) may lower the numbers needed for therapy. Read More

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http://dx.doi.org/10.1038/s41598-018-28346-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030170PMC
July 2018
6 Reads

Acellular Dermal Matrix in Immediate Expander/Implant Breast Reconstruction: A Multicenter Assessment of Risks and Benefits.

Plast Reconstr Surg 2018 09;142(3):414e

Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000004656DOI Listing
September 2018

Reconstruction of Microtia Using a Single Expanded Postauricular Flap without Skin Grafting: Experience of 683 Cases.

Plast Reconstr Surg 2018 07;142(1):170-179

Beijing and Chengdu, People's Republic of China From the Auricular Plastic and Reconstructive Surgery Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences Peking Union Medical College; and Bravou Plastic Surgery Hospital.

Background: Auricular reconstruction of congenital microtia patients is a huge challenge for plastic surgeons worldwide. Current techniques usually use a retroauricular fascial flap with skin grafting, often proving to be color mismatched, complex, and with visible scarring. An uncomplicated strategy that results in minimal scarring and a good color match is still required, especially for Asian patients. Read More

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http://dx.doi.org/10.1097/PRS.0000000000004493DOI Listing