2,762 results match your criteria Skin Tissue Expansion


Autologous Platelet-Rich Plasma (CuteCellTM PRP) Safely Boosts In Vitro Human Fibroblast Expansion.

Tissue Eng Part A 2019 Mar 21. Epub 2019 Mar 21.

University Hospitals of Geneva, Department of Plastic, Reconstructive & Aesthetic Surgery, , Rue Gabrielle-Perret-Gentil , Geneva, Switzerland , 1211.

Nowadays autologous fibroblast application for skin repair presents an important clinical interest. In most cases, in vitro skin cell culture is mandatory. However, cell expansion using xenogeneic or allogenic culture media presents some disadvantages, such as the risk of infection transmission or slow cell expansion. Read More

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http://dx.doi.org/10.1089/ten.TEA.2018.0335DOI Listing
March 2019
1 Read

PD-1 blockade following isolated limb perfusion with vaccinia virus prevents local and distant relapse of soft-tissue sarcoma.

Clin Cancer Res 2019 Mar 18. Epub 2019 Mar 18.

Skin Unit, Royal Marsden Hospital.

Purpose: The prevention and treatment of metastatic sarcoma are areas of significant unmet need. Immune checkpoint inhibitor monotherapy has shown little activity in sarcoma and there is great interest in identifying novel treatment combinations that may augment responses. and , we investigated the potential for an oncolytic vaccinia virus (GLV-1h68) delivered using isolated limb perfusion (ILP) to promote anti-tumour immune responses and augment response to PD-1 blockade in sarcoma. Read More

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http://clincancerres.aacrjournals.org/lookup/doi/10.1158/107
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http://dx.doi.org/10.1158/1078-0432.CCR-18-3767DOI Listing
March 2019
1 Read

A Rare Chest Wall Deformity after Usage of a Tissue Expander for Breast Reconstruction.

Plast Reconstr Surg Glob Open 2018 Nov 1;6(11):e1950. Epub 2018 Nov 1.

Department of Plastic and Reconstructive Surgery, Cancer Institute Hospital, Tokyo, Japan.

A 2-stage breast reconstruction using a breast tissue expander and prosthesis is a simple method of breast reconstruction with little donor-site morbidity and short surgery time. In this report, we present a rare case of chest wall deformity, which appeared during breast skin expansion with tissue expander. We present a case of a 31-year-old woman who underwent a 2-stage breast reconstruction with a tissue expander and breast prosthesis. Read More

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http://dx.doi.org/10.1097/GOX.0000000000001950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414119PMC
November 2018

Manufacturing of human Wharton's jelly stem cells for clinical use: selection of serum is important.

Cytotherapy 2019 Mar 15. Epub 2019 Mar 15.

Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore. Electronic address:

Background: Human Wharton's jelly-derived mesenchymal stromal cells (hWJSCs) have gained considerable attention for their use in cell therapy. Many of these applications would require manufacturing of millions of hWJSCs. It is, therefore, necessary to develop a Good Manufacturing Practice (GMP)-compliant hWJSC expansion protocol, allowing the generation of a large quantity of cells to meet both clinical and regulatory requirements. Read More

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http://dx.doi.org/10.1016/j.jcyt.2019.02.008DOI Listing

In vivo expansion and regeneration of full-thickness functional skin with an autologous homologous skin construct: Clinical proof of concept for chronic wound healing.

Int Wound J 2019 Mar 13. Epub 2019 Mar 13.

Department of Research and Development, PolarityTE, Inc., Salt Lake City, Utah.

A new cell-tissue technology uses a patient's skin to create an in vivo expanding and self-organising full-thickness skin autograft derived from potent cutaneous appendages. This autologous homologous skin construct (AHSC) is manufactured from a small full-thickness skin harvest obtained from an uninjured area of the patient. All the harvested tissue is incorporated into the AHSC including the endogenous regenerative cellular populations responsible for skin maintenance and repair, which are activated during the manufacturing process. Read More

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http://dx.doi.org/10.1111/iwj.13109DOI Listing

Inflammation and Lymphatic Function.

Front Immunol 2019 26;10:308. Epub 2019 Feb 26.

Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland.

The lymphatic vasculature plays a crucial role in regulating the inflammatory response by influencing drainage of extravasated fluid, inflammatory mediators, and leukocytes. Lymphatic vessels undergo pronounced enlargement in inflamed tissue and display increased leakiness, indicating reduced functionality. Interfering with lymphatic expansion by blocking the vascular endothelial growth factor C (VEGF-C)/vascular endothelial growth factor receptor 3 (VEGFR-3) signaling axis exacerbates inflammation in a variety of disease models, including inflammatory bowel disease (IBD), rheumatoid arthritis and skin inflammation. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399417PMC
February 2019
3 Reads

Sweat gland organoids contribute to cutaneous wound healing and sweat gland regeneration.

Cell Death Dis 2019 Mar 11;10(3):238. Epub 2019 Mar 11.

Stem Cell and Tissue Engineering Lab, Institute of Health Service and Transfusion Medicine, 100850, Beijing, China.

Sweat glands perform a vital thermoregulatory function in mammals. Like other skin components, they originate from epidermal progenitors. However, they have low regenerative potential in response to injury. Read More

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http://www.nature.com/articles/s41419-019-1485-5
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http://dx.doi.org/10.1038/s41419-019-1485-5DOI Listing
March 2019
1 Read

Use of Vacuum-Assisted Wound Closure and Tissue Expansion in Revision Cranioplasty for a Large-Sized Composite Defect in a Child.

J Craniofac Surg 2019 Feb 9. Epub 2019 Feb 9.

Department No. 16 of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Cranioplasty is generally defined as the procedure to reconstruct cranial bone defect arising from congenital or acquired process. As a surgical remedy, it can restore the function of original anatomical structure and produce a satisfactory cosmetic outcome. Although with available options for treating pediatric cranioplasty, large format (ie, >25 cm) cranioplasty of the aborted one with a composite defect is still a challenging procedure for plastic surgeons. Read More

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http://dx.doi.org/10.1097/SCS.0000000000005181DOI Listing
February 2019

Single stage, direct to implant pre-pectoral breast reconstruction.

Gland Surg 2019 Feb;8(1):53-60

Department of Surgery, Rush University Medical Center, Chicago, IL, USA.

Background: Given the current trends in skin preservation during mastectomy, improved biofilm reduction algorithms, and advancements in tissue bioengineering and perfusion assessment, acellular dermal matrix (ADM)-reinforced single stage, direct to implant insertion in the pre-pectoral space has become a viable alternative to two-stage expander-based, sub-pectoral reconstruction.

Methods: The authors performed a retrospective review of their pre-pectoral cases evaluating outcomes for all single stage, direct to implant procedures. Outcomes reviewed included hematoma, seroma, infection, device loss or change, animation deformity and revisional procedures such as fat grafting. Read More

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http://dx.doi.org/10.21037/gs.2018.10.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378250PMC
February 2019

The Future of Facial Fat Grafting.

J Craniofac Surg 2019 Feb 21. Epub 2019 Feb 21.

Department of Plastic Surgery.

Fat grafting was first described in the early 20th century but for many years remained a relatively underused technique due to the unreliability of long-term volume expansion. Significant improvements in reliability have been made in the last 2 decades and there is a large body of literature pertaining to extraction, processing and injection methods to obtain more lasting effects. However, volume loss and graft resorption remain a major challenge in the long term and lead to unpredictability in results. Read More

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http://dx.doi.org/10.1097/SCS.0000000000005274DOI Listing
February 2019
1 Read

TSA restores hair follicle-inductive capacity of skin-derived precursors.

Sci Rep 2019 Feb 27;9(1):2867. Epub 2019 Feb 27.

State Key Laboratory of Chemical Oncogenomics, and the the Shenzhen Key Laboratory of Health Sciences and Technology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.

The genesis of the hair follicle relies on signals derived from mesenchymal cells in the dermis during skin morphogenesis and regeneration. Multipotent skin-derived precursors (SKPs), which exhibit long term proliferation potential when being cultured in spheroids, have been shown to induce hair genesis and hair follicle regeneration in mice, implying a therapeutic potential of SKPs in hair follicle regeneration and bioengineering. However, the hair-inductive property of SKPs declines progressively upon ex vivo culture expansion, suggesting that the expressions of the genes responsible for hair induction are epigenetically unstable. Read More

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http://dx.doi.org/10.1038/s41598-019-39394-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393485PMC
February 2019
2 Reads

Histological changes of immediate skin expansion of the distal limb of rats.

Vet World 2018 Dec 20;11(12):1706-1711. Epub 2018 Dec 20.

Department of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Aim: Tissue expansion is an applicable technique to reconstruct many surgical defects. The aim of this research was to evaluate the histological changes caused by immediate skin tissue expansion in rats as an animal model.

Materials And Methods: Immediate skin tissue expansion in 18 adult female rats was performed using three different sizes (small, medium, and big) of polymethylmethacrylate tissue expanders at the dorsal surface of the metatarsal area of the right limb. Read More

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http://dx.doi.org/10.14202/vetworld.2018.1706-1711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362334PMC
December 2018
1 Read

Leukoencephalopathy in RIN2 syndrome: Novel mutation and expansion of clinical spectrum.

Eur J Med Genet 2019 Feb 13. Epub 2019 Feb 13.

Myelin Disorders Clinic, Pediatric Neurology Division, Children's Medical Center, Pediatrics Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

RIN2 syndrome also known as MACS syndrome is a rare autosomal recessive connective tissue disorder caused by RIN2 mutations and is accompanied by following clinical features: macrocephaly, coarsening of facial features, downward slanting palpebral fissures, Puffy droopy eyelids, full everted lips, soft redundant skin especially in face, gum hypertrophy, irregular dentition, sparse scalp hair, skeletal problems, joint hypermobility and scoliosis. RIN2 gene encodes the RAS and RAB interactor 2 and biallelic mutations in this gene cause cell trafficking dysfunction. Here we reported the eleventh patient of RIN2 syndrome in a 4 yr-old boy, from Tehran, Iran as the youngest reported patient so far. Read More

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http://dx.doi.org/10.1016/j.ejmg.2019.02.002DOI Listing
February 2019

Serial Excision for the Treatment of Giant Congenital Melanocytic Nevus: The Vietnamese Way.

Open Access Maced J Med Sci 2019 Jan 26;7(2):231-233. Epub 2019 Jan 26.

University of Rome G. Marconi, Rome, Italy.

Aim: To investigate the efficacy of plastic surgery in the treatment of giant congenital melanocytic nevus (GCMN).

Methods: We enrolled 20 patients with 44 lesions and performed one of the following procedures: serial excision, skin grafting, tissue expansion, primary skin closure, distant flap, and adjacent flap. We assessed the outcome at 10 days and 6 months after surgery. Read More

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http://dx.doi.org/10.3889/oamjms.2019.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364729PMC
January 2019

Passot-Type Immediate Breast Reconstruction Obviates the Use of Aceullular Dermal Matrix in Grades 2 and 3 Ptosis.

Ann Plast Surg 2019 Jan 28. Epub 2019 Jan 28.

From the Department of Plastic Surgery, University of Tennessee College of Medicine, Chattanooga, TN.

Purpose: Acellular dermal matrices (ADMs) are commonly used for immediate breast reconstruction after skin-sparing mastectomy (SSM). Regnault grades 2 and 3 ptotic breasts feature significant mastectomy flap redundancy that may be incorporated into the reconstruction. This allows surgeons to use patients' de-epithelialized inferior dermal pedicle instead of ADM. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001792DOI Listing
January 2019

The Nematode Caenorhabditis elegans as a Model Organism to Study Metabolic Effects of ω-3 Polyunsaturated Fatty Acids in Obesity.

Adv Nutr 2019 Jan;10(1):165-178

Departments of Plant and Soil Science, Texas Tech University, Lubbock, TX.

Obesity is a complex disease that is influenced by several factors, such as diet, physical activity, developmental stage, age, genes, and their interactions with the environment. Obesity develops as a result of expansion of fat mass when the intake of energy, stored as triglycerides, exceeds its expenditure. Approximately 40% of the US population suffers from obesity, which represents a worldwide public health problem associated with chronic low-grade adipose tissue and systemic inflammation (sterile inflammation), in part due to adipose tissue expansion. Read More

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http://dx.doi.org/10.1093/advances/nmy059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370270PMC
January 2019
1 Read

Soft-Tissue Expansion beforeTotal Knee Arthroplasty:A Report of Two Cases.

J Orthop Case Rep 2018 Jul-Aug;8(4):20-24

Department of Orthopedics and Traumatology, Institute of Orthopedics "Carlos E. Ottolenghi," Hospital Italiano deBuenos Aires, Potosi 4215, and 1199 Buenos Aires, Argentina.

Introduction: Wound complications are a feared complication following total knee arthroplasty (TKA). Furthermore, it is important to avoid underestimation in the pre-operative planning as it may lead to catastrophic TKA failure. Soft-tissue expansion (STE) has been indicated when insufficient or inadequate soft-tissue coverage is present before TKA as an alternative to optimize soft-tissue management. Read More

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http://dx.doi.org/10.13107/jocr.2250-0685.1140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343565PMC
January 2019
6 Reads

Tissue expanders; review of indications, results and outcome during 15 years' experience.

Burns 2019 Jan 23. Epub 2019 Jan 23.

Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

Background: Tissue expanders (TE) are frequently used worldwide. In this study we surveyed outcome of our patients retrospectively during 15 years.

Materials And Methods: We had 1105 patients for whom 3059 TEs have been used. Read More

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http://dx.doi.org/10.1016/j.burns.2018.11.017DOI Listing
January 2019
1 Read

Xeno-free expansion of adult keratinocytes for clinical application: the use of human-derived feeder cells and serum.

Cell Tissue Res 2019 Jan 21. Epub 2019 Jan 21.

Skin Bioengineering Laboratory, Victorian Adult Burns Service, Alfred Hospital, 89 Commercial Road, Melbourne, Victoria, 3181, Australia.

Cultured epithelial autograft (CEA) was the birth of skin tissue engineering and encompassed methodologies for the isolation and expansion of autologous basal keratinocytes for burn treatment that are still practiced at some specialised units around the world. One of the limitations of CEA, however, is the reliance on animal-derived material during the manufacturing process and despite all efforts to date, no xeno-free alternative with proven efficacy has been reported. Here, we investigate whether human-derived fibroblast feeder cells and human serum can sufficiently and effectively provide a suitable microenvironment for adult keratinocyte isolation and expansion. Read More

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http://dx.doi.org/10.1007/s00441-018-02986-5DOI Listing
January 2019
3.565 Impact Factor

Macrophages are necessary for skin regeneration during tissue expansion.

J Transl Med 2019 Jan 21;17(1):36. Epub 2019 Jan 21.

Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, 127 Chanle West Road, Xi'an, 710032, Shaanxi Province, China.

Background: Tissue expansion is a procedure that promotes skin regeneration by mechanical stretch. During the stress and relaxation cycle, the skin undergoes a repeated microtrauma which triggers an immune response leading to the recruitment of macrophages to repair the damaged tissue. Macrophages have been found to be necessary for tissue repair and wound healing, but their effects on skin regeneration during mechanical stretch remain unclear. Read More

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https://translational-medicine.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12967-019-1780-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341552PMC
January 2019
10 Reads

Correction of Tanzer Type IIB Constricted Ears via Z-shaped Double V-Y Advancement Flaps.

Ann Plast Surg 2019 Mar;82(3):284-288

From the Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: Correction of Tanzer type IIB constricted ears requires sufficient expansion of the auricular skin to cover the reconstructed cartilaginous framework. To obtain an adequately sized skin cover, skin flap transposition from periauricular donor sites has been described; however, it remains challenging to obtain enough skin to enable satisfactory reconstruction. Herein, we describe a novel method that uses 2 local flaps comprising superoauricular, retroauricular, and preauricular soft tissue to expand the skin cover and correct Tanzer type IIB deformity. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001750DOI Listing
March 2019
4 Reads

Transplantation of Adipose-Derived Stem Cells Before Flap Expansion and After Expanded Flap Elevation Result in Different Effects.

Ann Plast Surg 2019 Feb;82(2):237-244

Columbia University Mailman School of Public Health, New York, NY.

Background: Studies of using mesenchymal stem cells to assist skin and soft tissue expansion have shown that stem cells can improve expansion efficiency through promoting tissue regeneration. However, the issue that whether the flap viability is also improved is unknown.

Methods: Sixteen pigs were equally divided into 2 groups. Read More

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http://Insights.ovid.com/crossref?an=00000637-201902000-0002
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http://dx.doi.org/10.1097/SAP.0000000000001736DOI Listing
February 2019
3 Reads

Quantitative assessment and risk factors for chest wall deformity resulting from tissue expansion for breast reconstruction.

Breast Cancer 2019 Jan 2. Epub 2019 Jan 2.

Department of Oral and Maxillofacial Surgery, and Plastic Surgery, Gunma University Graduate School of Medicine, 3-39-22, Showa-machi, Maebashi, Gunma, 371-8511, Japan.

Background: Chest wall deformity after tissue expansion for breast reconstruction is less recognized than complications such as infection, hematoma, skin necrosis and capsular contracture. However, the condition should not be discounted because pain, rib fracture and dyspnea may occur in severe cases. The aim of this study is to evaluate the extent of chest wall deformity quantitatively using computed tomography (CT) and to identify risk factors for this condition after tissue expansion. Read More

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http://dx.doi.org/10.1007/s12282-018-00942-3DOI Listing
January 2019
1 Read

Full Thickness Skin Expansion in a Newly Developed Reactor and Evaluation of Auto-Grafting Efficiency of the Expanded Skin Using Yucatan Pig Model.

Tissue Eng Regen Med 2018 Oct 1;15(5):629-638. Epub 2018 Sep 1.

Biomedical Research Institute, Joint Institute for Regenerative Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, 41944 Republic of Korea.

Background: Skin grafts are required in numerous clinical procedures, such as reconstruction after skin removal and correction of contracture or scarring after severe skin loss caused by burns, accidents, and trauma. The current standard for skin defect replacement procedures is the use of autologous skin grafts. However, donor-site tissue availability remains a major obstacle for the successful replacement of skin defects and often limits this option. Read More

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http://dx.doi.org/10.1007/s13770-018-0154-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171704PMC
October 2018
1 Read

Staged reconstructive treatment for extensive irregular cicatricial alopecia after burn.

Medicine (Baltimore) 2018 Dec;97(52):e13522

Department of Plastic Surgery, Affiliated Hangzhou First People's Hospital.

For extensive irregular cicatricial alopecia after burn, effective and pleasing restoration of hair-bearing scalp remains challenging. In this article, the authors presented staged reconstructive treatment for extensive irregular cicatricial alopecia with the goal to achieve better and reliable results. A retrospective review of staged reconstructive treatment performed in 16 patients with extensive irregular cicatricial alopecia after burn was conducted. Read More

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http://Insights.ovid.com/crossref?an=00005792-201812280-0000
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http://dx.doi.org/10.1097/MD.0000000000013522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314779PMC
December 2018
4 Reads

Advances in diagnosis of mastocytosis and hypereosinophilic syndrome.

Semin Hematol 2019 Jan 28;56(1):22-29. Epub 2018 May 28.

Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508.

Mastocytosis and hypereosinophilic syndrome is very rare neoplastic hematopoietic diseases. Mastocytosis is characterized by expansion and accumulation of clonal tissue mast cells in skin and/or various internal organs, while hypereosinophilic syndrome manifests with an increased number of eosinophils in the peripheral blood and tissue damage. These diseases represent a diagnostic challenge, since they can have overlapping clinical and pathologic features. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00371963183003
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http://dx.doi.org/10.1053/j.seminhematol.2018.05.005DOI Listing
January 2019
6 Reads

Use of Tissue Expander for Contracted Scarred Saddle Deformity Rhinoplasty.

Facial Plast Surg 2019 Feb 19;35(1):68-72. Epub 2018 Dec 19.

Division of Facial Plastic Surgery, Department of Otolaryngology, Loma Linda University Health, Loma Linda, California.

The main purpose of this article is to present an alternative technique for the reconstruction of saddle nose deformity in patients with severely scarred or contracted soft tissue envelopes. In this single surgeon case series, the authors present a two-staged reconstruction performed on four adult patients with saddle nose deformities and contracted soft tissue envelope stemming from a variety of etiologic factors including vasculitis, sarcoidosis, and trauma. Stage one involved placement of a 1 × 4 cm tissue expander along the nasal dorsum through anterior scalp and endonasal incisions. Read More

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http://dx.doi.org/10.1055/s-0038-1675632DOI Listing
February 2019
3 Reads

Delivery of External Volume Expansion through Microdeformational Interfaces Safely Induces Angiogenesis in a Murine Model of Intact Diabetic Skin with Endothelial Cell Dysfunction.

Plast Reconstr Surg 2019 Feb;143(2):453-464

Boston, Mass.; and Beijing, People's Republic of China From the Tissue Engineering and Wound Healing Laboratory, Department of Surgery, Division of Plastic Surgery, Brigham and Women's Hospital and Harvard Medical School; Peking University People's Hospital; and the Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Background: External volume expansion promotes angiogenesis in tissues by inducing a subcritical hypoxia, mechanically stimulating endothelia, and creating mild inflammation. Application of external volume expansion to less-vascularized tissues with endothelial cell dysfunction poses a risk for ineffectiveness or ischemic damage. The authors investigated the effects of external volume expansion on a murine model of type 2 diabetes mellitus and tested whether the adoption of microdeformational interfaces optimizes its angiogenic properties while limiting complications to tissues. Read More

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http://dx.doi.org/10.1097/PRS.0000000000005267DOI Listing
February 2019
2 Reads

Treatment With Topical Deferoxamine Improves Cutaneous Vascularity and Tissue Pliability in an Irradiated Animal Model of Tissue Expander-Based Breast Reconstruction.

Ann Plast Surg 2019 Jan;82(1):104-109

Division of Plastic Surgery, University of Kentucky, Lexington, KY.

Purpose: Postmastectomy radiation therapy is an important component of the multimodality approach to later-stage breast cancers. Unfortunately, despite its proven survival benefits, postmastectomy radiation therapy is deleterious to the skin and soft tissue, causing increased complications and worse aesthetic outcomes after breast reconstruction.There is currently no effective pharmaceutical agent to mitigate the soft tissue fibrosis and hypovascularity associated with soft tissue radiation. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001655DOI Listing
January 2019
6 Reads

Immediate breast reconstruction in high-risk cases using an anatomically shaped permanent expandable implant.

J Plast Reconstr Aesthet Surg 2019 Mar 13;72(3):401-409. Epub 2018 Nov 13.

Plastic and Reconstructive Breast Surgery Unit, Department of Plastic Surgery, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, 6 Weizmann St., Tel-Aviv 6423906, Israel. Electronic address:

Background: Anatomically shaped permanent expandable implants are dual-chambered devices, which allow their postoperative expansion for the adjustment of volume. Their use is optional when 1-stage immediate breast reconstruction (IBR) direct to permanent implant is not feasible. The aim of this study is to present one center's long-term experience with the anatomical Becker expandable implant in a large series of patients who underwent either IBR or a salvage procedure for IBR with the device. Read More

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http://dx.doi.org/10.1016/j.bjps.2018.10.030DOI Listing

Experiences with a temporary synthetic skin substitute after decompressive craniectomy: a retrospective two-center analysis.

Acta Neurochir (Wien) 2019 Mar 4;161(3):493-499. Epub 2018 Dec 4.

Department of Neurosurgery, Trauma Center Murnau, Professor-Küntscher Straße 8, 82418, Murnau, Germany.

Background: Decompressive craniectomy is a commonly performed procedure. It reduces intracranial pressure, improves survival, and thus might have a positive impact on several neurosurgical diseases and emergencies. Sometimes primary skin closure is not possible due to cerebral herniation or extensive skin defects. Read More

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http://dx.doi.org/10.1007/s00701-018-3748-9DOI Listing
March 2019
4 Reads

Assisting Rapid Soft-Tissue Expansion with Adipose-Derived Stem Cells: An Experimental Study in a Pig Model.

Plast Reconstr Surg 2018 11;142(5):674e-684e

Beijing, People's Republic of China From the Head & Neck Aesthetic and Plastic Surgery Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College.

Background: Rapid tissue expansion has been attempted, aiming at shortening the period of conventional expansion. However, it has scarcely been clinically applied because of its drawbacks such as low expansion efficiency and tissue destruction. Adipose-derived stem cell transplantation is a promising therapeutic method in regenerative medicine. Read More

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http://dx.doi.org/10.1097/PRS.0000000000004884DOI Listing
November 2018
7 Reads

Microtia Reconstruction in Hemifacial Microsomia Patients: Three Framework Coverage Techniques.

Plast Reconstr Surg 2018 12;142(6):1558-1570

Seoul, Republic of Korea From the Seoul Center for Developmental Ear Anomalies and the Department of Plastic and Reconstructive Surgery, Korea University Anam Hospital.

Background: Microtia with hemifacial microsomia is difficult to treat because of skin volume deficiency. To provide further information for coverage techniques in microtia reconstruction, the authors have reviewed and analyzed patients who underwent surgery at their center.

Methods: A total 52 patients with microtia with hemifacial microsomia who underwent reconstruction between 2006 and 2016 were involved. Read More

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http://dx.doi.org/10.1097/PRS.0000000000005063DOI Listing
December 2018
1 Read

Reconstruction of a surgical defect in the popliteal fossa: A case report.

Int J Surg Case Rep 2018 1;53:228-230. Epub 2018 Nov 1.

Columbia University Medical Center, Department of Dermatology, New York, NY, 10032, United States. Electronic address:

Introduction: Surgical defects of the popliteal fossa pose a reconstructive challenge to the surgeon, given the intimate relationship of this area with the knee joint and underlying vital neuro-vasculature.

Case Presentation: An 88-year-old woman was treated for a biopsy proven invasive squamous cell carcinoma of the right popliteal fossa with Mohs micrographic surgery. The resultant defect (4. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22102612183049
Publisher Site
http://dx.doi.org/10.1016/j.ijscr.2018.10.070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232581PMC
November 2018
17 Reads

Reconstruction of the Forehead and Scalp.

Facial Plast Surg Clin North Am 2019 Feb;27(1):85-94

Department of Otolaryngology-Head and Neck Surgery, New York University School of Medicine, 240 East 38th Street, 14th Floor, New York, NY 10016, USA. Electronic address:

Tissue inelasticity and the hair-bearing nature of the scalp and forehead pose unique challenges during reconstruction. A thorough understanding of the surgical anatomy of the scalp and forehead is paramount for optimal reconstructive outcomes. Primary wound closure is usually preferred over secondary intention healing and skin grafting. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10647406183007
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http://dx.doi.org/10.1016/j.fsc.2018.08.009DOI Listing
February 2019
20 Reads

The proliferation and clonal migration of B cells in the systemic and mucosal tissues of channel catfish suggests there is an interconnected mucosal immune system.

Fish Shellfish Immunol 2019 Jan 7;84:1134-1144. Epub 2018 Nov 7.

Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS, 39216-4505, USA.

IgM transcripts from different mucosal and systemic tissues from a single adult channel catfish have been evaluated. Arrayed heavy chain cDNA libraries from each of these different mucosal and systemic tissues were separately constructed, hybridized with VH family specific probes and a variety of approaches were used to define their structural relationships. Baseline hybridization studies indicated that the tissue libraries had different VH expression patterns, and sequencing studies indicated this was not simply due to varying proportions of the same B cell population. Read More

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http://dx.doi.org/10.1016/j.fsi.2018.11.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335153PMC
January 2019
3 Reads

Additive effect of bFGF and selenium on expansion and paracrine action of human amniotic fluid-derived mesenchymal stem cells.

Stem Cell Res Ther 2018 11 8;9(1):293. Epub 2018 Nov 8.

Laboratory of Cell Function Regulation, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-go, Seoul, 02841, Republic of Korea.

Background: Mesenchymal stem cell-derived conditioned medium (MSC-CM) has emerged as a promising cell-free tool for restoring degenerative diseases and treating traumatic injuries. The present study describes the effect of selenium as a reactive oxygen species (ROS) scavenger and its additive effect with basic fibroblast growth factor (bFGF) on in vitro expansion of amniotic fluid (AF)-MSCs and the paracrine actions of AF-MSC-CM as well as the associated cellular and molecular mechanisms.

Methods: In this study, we obtained CM from human AF-MSCs cultured with selenium. Read More

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http://dx.doi.org/10.1186/s13287-018-1058-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225588PMC
November 2018

Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin.

JCI Insight 2018 11 2;3(21). Epub 2018 Nov 2.

Department of Dermatology, UCSD, La Jolla, California, USA.

In this study we evaluated the role of hyaluronan (HA) in reactive adipogenesis, a local expansion of preadipocytes that provides host defense by release of antimicrobial peptides. We observed that HA accumulated during maturation of adipocytes in vitro and was associated with increased expression of preadipocyte factor 1, zinc finger protein 423, and early B cell factor 1. Although HA is normally abundant in the extracellular matrix, a further increase in HA staining occurred in mice at sites of reactive adipogenesis following injury of colon by dextran sodium sulfate or injury of skin from infection with Staphylococcus aureus. Read More

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http://dx.doi.org/10.1172/jci.insight.123072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6238730PMC
November 2018

Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes.

Pediatr Surg Int 2019 Jan 31;35(1):121-127. Epub 2018 Oct 31.

Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032, Zurich, Switzerland.

Aim Of The Study: The use of autologous bio-engineered dermo-epidermal skin substitutes (DESS) yields a pivotal opportunity to cover large skin defects in human patients. These skin grafts consist of both epidermal and dermal compartments necessary for robust and permanent functional wound closure. In this study, we investigated the impact of mesenchymal cells derived from different body site origins on the expression pattern of diverse markers within DESS. Read More

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http://dx.doi.org/10.1007/s00383-018-4383-5DOI Listing
January 2019

Oncogenic activation of PI3K induces progenitor cell differentiation to suppress epidermal growth.

Nat Cell Biol 2018 Nov 22;20(11):1256-1266. Epub 2018 Oct 22.

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Oncogenic lesions are surprisingly common in morphologically and functionally normal human skin. However, the cellular and molecular mechanisms that suppress their cancer-driving potential to maintain tissue homeostasis are unknown. By employing assays for the direct and quantitative assessment of cell fate choices in vivo, we show that oncogenic activation of PI3K-AKT, the most commonly activated oncogenic pathway in cancer, promotes the differentiation and cell cycle exit of epidermal progenitors. Read More

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http://dx.doi.org/10.1038/s41556-018-0218-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291208PMC
November 2018
1 Read

Reconstruction of Facial Defects with Three-Stage Frontal Expanded Bipedicled Flaps.

J Craniofac Surg 2019 Jan;30(1):175-177

Plastic Surgery Hospital, Peking Union Medical College, Beijing, China.

Background: The aim of the present study was the therapeutic effect of the frontal expanded bipedicled flaps that are used in the reconstruction of facial defects. Patients were treated with mostly unilateral facial defects which affected temple area and cross the midline with frontal expanded flaps to investigate the therapeutic effect of reconstruction of facial defects with frontal expanded bipedicled flaps from June 2012 to August 2017 and 2 patients were discussed in this study.

Methods: The surgery procedure was divided into 3 stages. Read More

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http://dx.doi.org/10.1097/SCS.0000000000004852DOI Listing
January 2019

High-Salt Diet Causes Expansion of the Lymphatic Network and Increased Lymph Flow in Skin and Muscle of Rats.

Arterioscler Thromb Vasc Biol 2018 Sep;38(9):2054-2064

From the Department of Biomedicine, University of Bergen, Norway (T.V.K., E.N., J.H., T.R., O.T., H.W.).

Objective- A commonly accepted pivotal mechanism in fluid volume and blood pressure regulation is the parallel relationship between body Na and extracellular fluid content. Several recent studies have, however, shown that a considerable amount of Na can be retained in skin without commensurate water retention. Here, we asked whether a salt accumulation shown to result in VEGF (vascular endothelial growth factor)-C secretion and lymphangiogenesis had any influence on lymphatic function. Read More

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http://dx.doi.org/10.1161/ATVBAHA.118.311149DOI Listing
September 2018
11 Reads

Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling.

Cell Rep 2018 Oct;25(3):598-610.e5

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63108, USA; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108, USA. Electronic address:

Despite substantial self-renewal capability in vivo, epithelial stem and progenitor cells located in various tissues expand for a few passages in vitro in feeder-free condition before they succumb to growth arrest. Here, we describe the EpiX method, which utilizes small molecules that inhibit PAK1-ROCK-Myosin II and TGF-β signaling to achieve over one trillion-fold expansion of human epithelial stem and progenitor cells from skin, airway, mammary, and prostate glands in the absence of feeder cells. Transcriptomic and epigenomic studies show that this condition helps epithelial cells to overcome stresses for continuous proliferation. Read More

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http://dx.doi.org/10.1016/j.celrep.2018.09.072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6284236PMC
October 2018
8 Reads

New economical and simple device for intraoperative expansion on small and medium sized soft tissue defects.

Arch Craniofac Surg 2018 Sep 20;19(3):235-239. Epub 2018 Sep 20.

Department of Plastic and Reconstructive Surgery, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.

Intraoperative expansion has been used to cover small to large defects without disadvantages of the conventional tissue expanders. Various materials, for example, expanders and Foley catheters are being used. We introduce a new, convenient and economical device immediately available in the operating room, according to the defect size for intraoperative expansion, with latex gloves or balloons. Read More

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http://e-acfs.org/journal/view.php?doi=10.7181/acfs.2018.019
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http://dx.doi.org/10.7181/acfs.2018.01998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177679PMC
September 2018
8 Reads

A novel technique for large and ptotic breast reconstruction using a latissimus dorsi myocutaneous flap set at the posterior aspect, combined with a silicone implant, following tissue expander surgery.

Arch Plast Surg 2018 Sep 15;45(5):484-489. Epub 2018 Sep 15.

Department of Plastic and Reconstructive Surgery, Keio University, Tokyo, Japan.

Large and ptotic breast reconstruction in patients who are not candidates for a transverse rectus abdominalis myocutaneous flap and revision surgery for the contralateral breast remains challenging. We developed a novel breast reconstruction technique using a latissimus dorsi myocutaneous (LD m-c) flap set at the posterior aspect of the reconstructed breast, combined with an anatomical silicone breast implant (SBI), following tissue expander surgery. We performed the proposed technique in four patients, in whom the weight of the resected tissue during mastectomy was >500 g and the depth of the inframammary fold (IMF) was >3 cm. Read More

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http://e-aps.org/journal/view.php?doi=10.5999/aps.2017.01781
Publisher Site
http://dx.doi.org/10.5999/aps.2017.01781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177628PMC
September 2018
3 Reads

Mid-axillary pacemaker re-implantation after contralateral pocket infection in an emaciated elderly case.

J Cardiol Cases 2018 Aug 9;18(2):70-73. Epub 2018 Jun 9.

Department of Cardiovascular Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.

The number of implantations of cardiac implantable electrophysiological devices (CIEDs) has increased over the past several years. However, the aging population and expansion of indications for CIEDs have led to an increase in associated infections. We experienced a case of a 99-year-old man presenting with skin erosion at the pocket site, where a 6-month-old implantable pacemaker was replaced. Read More

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http://dx.doi.org/10.1016/j.jccase.2018.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149616PMC
August 2018
2 Reads

Changes in the Biomechanical Properties of Human Skin in Hyperthermic and Hypothermic Ranges.

Wounds 2018 Sep;30(9):257-262

Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tuebingen, Germany.

Introduction: The prevalence of thermal skin injuries is high. Despite new research findings, skin burns and acute cold-contact injuries, together with resulting tissue damage, are not entirely understood. In particular, little is known about how these types of injuries alter the biomechanical properties of skin. Read More

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September 2018
17 Reads

Current Approaches to Prepectoral Breast Reconstruction.

Plast Reconstr Surg 2018 10;142(4):871-880

Washington, D.C. From Georgetown University Hospital.

Prepectoral prosthetic breast reconstruction has become an acceptable option for women following mastectomy. Benefits include no animation deformity, absence of pectoralis major muscle spasm, and less pain and discomfort. Important aspects of prepectoral reconstruction include working with breast surgeons that are adept at performing an optimal mastectomy. Read More

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http://dx.doi.org/10.1097/PRS.0000000000004802DOI Listing
October 2018

Body Wall Reconstruction for Conjoined Twins: Our Experience and Lessons Learned.

Ann Plast Surg 2018 Dec;81(6S Suppl 1):S66-S70

From the Department of Plastic Surgery, Union Hospital, and.

Background: Conjoined twinning is a rare congenital malformation, and the resultant huge body wall defects after separation of conjoined twins represent a real challenge to surgeons.

Methods: From 2004 to 2009, authors performed body wall reconstruction for 2 pairs of thoraco-omphalopagus twins and 1 pair of ischiopagus tetrapus twins. Before separation, the techniques of tissue expansion and tractive training were adopted to acquire sufficient skin for final coverage. Read More

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http://dx.doi.org/10.1097/SAP.0000000000001609DOI Listing
December 2018
4 Reads