Toxicol In Vitro 2022 Jun 25:105425. Epub 2022 Jun 25.
National Institute of Metrology, Quality and Technology (Inmetro), Directorate of Scientific Metrology applied to Life Sciences, Av. Nossa Senhora das Graças, 50, Xerém, CEP 25250-020 Duque de Caxias, RJ, Brazil.
Usually, if percutaneous absorption tests are conducted in accordance with OECD Guideline 428, in vitro determination is accepted by mainly regulatory agencies. In this paper, we focus on the lack of comparability of the results regarding the permeation parameter/flow rate, although it is widely discussed in the literature. This work sought to evaluate the absorption of caffeine using Franz-type diffusion cell with porcine ear skin samples, varying the storage duration and the way to handle them. Read More