The sphingosine 1-phosphate breakdown product, (2E)-hexadecenal, forms protein adducts and glutathione conjugates in vitro.
- Fabian Schumacher,
- Corinna Neuber,
- Hannah Finke,
- Kai Nieschalke,
- Jessica Baesler,
- Erich Gulbins,
- Burkhard Kleuser
J Lipid Res 2017 Aug 6;58(8):1648-1660. Epub 2017 Jun 6.
Department of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, 14558 Nuthetal, Germany
Sphingosine 1-phosphate (S1P), a bioactive lipid involved in various physiological processes such as cell proliferation and apoptosis, can be irreversibly cleaved by S1P lyase, yielding phosphoethanolamine and (2E)-hexadecenal (2EHD). The latter metabolite, an α,β-unsaturated fatty aldehyde, may be susceptible to nucleophilic attack by cellular biomolecules. Hence, we studied whether 2EHD forms reaction products with GSH and proteins in vitro. Read More