10,821 results match your criteria Serratia


The Effects of a Bacterial Endotoxin on Behavior and Sensory-CNS-Motor Circuits in .

Insects 2019 Apr 22;10(4). Epub 2019 Apr 22.

Department of Biology, University of Kentucky, Lexington, KY 40506-0225, USA.

The effect of bacterial sepsis on animal behavior and physiology is complex due to direct and indirect actions. The most common form of bacterial sepsis in humans is from gram-negative bacterial strains. The endotoxin (lipopolysaccharide, LPS) and/or associated peptidoglycans from the bacteria are the key agents to induce an immune response, which then produces a cascade of immunological consequences. Read More

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http://dx.doi.org/10.3390/insects10040115DOI Listing

Environmental potassium regulates bacterial flotation, antibiotic production and turgor pressure in Serratia through the TrkH transporter.

Environ Microbiol 2019 Apr 22. Epub 2019 Apr 22.

University of Cambridge, Department of Biochemistry., Hopkins Building, Downing Site. Cambridge, CB2 1QW, UK.

Serratia sp. strain ATCC 39006 (S39006) can float in aqueous environments due to natural production of gas vesicles (GVs). Expression of genes for GV morphogenesis is stimulated in low oxygen conditions, thereby enabling migration to the air-liquid interface. Read More

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http://dx.doi.org/10.1111/1462-2920.14637DOI Listing

An optimized cocktail of chitinolytic enzymes to produce N,N'-diacetylchitobiose and N-acetyl-d-glucosamine from defatted krill by-products.

Int J Biol Macromol 2019 Apr 17. Epub 2019 Apr 17.

State Key Laboratory of Fine Chemical Engineering and School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

Defatted krill powder (DKP), the byproduct of krill oil industry, is a resource of biological macromolecules. Here, one bacterial protease, three bacterial chitinases and one insect N-acetyl-d-hexosaminidase were integratively used to produce peptide, N,N'-diacetylchitobiose [(GlcNAc)] and N-acetyl-d-glucosamine (GlcNAc) from DKP. First, alkaline protease was found to outperform neutral protease in deproteinizing DKP and the resultant krill peptides were rich in essential amino acids (41. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.114DOI Listing
April 2019
1 Read

The plant host induces antibiotic production to select the most beneficial colonizers.

Appl Environ Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Molecular Genetics, Weizmann Institute of Science

Microbial ecosystems tightly associated with a eukaryotic host are wide-spread in nature. The genetic and metabolic networks of the eukaryotic hosts and the associated microbes have co-evolved to form a symbiotic relationship. Both the Gram-positive and the Gram-negative can form biofilms on plant roots, and thus can serve as a model system for the study of interspecies interactions in a host-associated ecosystem. Read More

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http://aem.asm.org/lookup/doi/10.1128/AEM.00512-19
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http://dx.doi.org/10.1128/AEM.00512-19DOI Listing
April 2019
1 Read
3.668 Impact Factor

Assessing the efficiency in assisted depuration of coffee processing wastewater from mixed wild microbial selected inoculum.

Environ Monit Assess 2019 Apr 17;191(5):284. Epub 2019 Apr 17.

Department of Biology, Universidade Federal de Lavras. Campus Universitário, CEP: 37.200-000, Lavras, MG, Brazil.

This work evaluated the efficiency of bacterial bio-augmentation to the biological treatment of coffee processing wastewater (CPWW) in a pilot wastewater treatment plant (WTP). Biochemical oxygen demand (BOD) and chemical oxygen demand (COD) values were the basis for the treatment efficiency. Serratia marcescens CCMA 1010 and CCMA 1013, Corynebacterium flavescens CCMA 1006 and Acetobacter indonesiensis CCMA 1002 were previously selected. Read More

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http://dx.doi.org/10.1007/s10661-019-7398-zDOI Listing
April 2019
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Identification of CTX-M-15 and CTX-M-27 in Antibiotic-Resistant Gram-Negative Bacteria Isolated from Three Rivers Running in Central Italy.

Microb Drug Resist 2019 Apr 17. Epub 2019 Apr 17.

2 Department of Medical, Oral and Biotechnological Sciences, and Center of Excellence on Aging and Translational Medicine (CeSI-MeT), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.

The main goal of this study was to identify Gram-negative bacteria resistant to antibiotics, in particular β-lactams, in stream waters and effluents from urban wastewater treatment plants draining into Fino, Tavo, and Saline rivers of the Abruzzo region, Italy. Eight sampling sites were selected because they were the most contaminated by coliforms during previous sampling campaign. One sample for each site was collected for the detection of total and fecal coliforms, and species by Colilert-18 and Enterolert-E Quanti-Tray/2000. Read More

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http://dx.doi.org/10.1089/mdr.2019.0016DOI Listing

SME-4-producing Serratia marcescens from Argentina belonging to clade 2 of the S. marcescens phylogeny.

J Antimicrob Chemother 2019 Apr 16. Epub 2019 Apr 16.

EA7361 'Structure, dynamic, function and expression of broad spectrum β-lactamases', Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France.

Background: SME carbapenemases are increasingly reported, especially from North and South America. Here, we describe an SME-4-producing Serratia marcescens (SME-Sm) clinical isolate from Argentina and compare its genome with other SME-Sm and Sm isolates recovered from public databases.

Methods: Sm isolates were characterized by WGS using Illumina technology, susceptibility testing and MIC determination. Read More

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https://academic.oup.com/jac/advance-article/doi/10.1093/jac
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http://dx.doi.org/10.1093/jac/dkz115DOI Listing
April 2019
1 Read

Lead binding to wild metal-resistant bacteria analyzed by ITC and XAFS spectroscopy.

Environ Pollut 2019 Apr 9;250:118-126. Epub 2019 Apr 9.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A&F University, Yangling, 712100, China; CAS Center for Excellence in Quaternary Science and Global Change, Chinese Academy of Sciences, Xian, 710061, China. Electronic address:

Metal-resistant bacteria can survive exposure to high metal concentrations without any negative impact on their growth. Biosorption is considered to be one of the more effective detoxification mechanisms acting in most bacteria. However, molecular-scale characterization of metal biosorption by wild metal-resistant bacteria has been limited. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.03.123DOI Listing
April 2019
3 Reads

Functional and Genetic Diversity of Bacteria Associated with the Surfaces of Agronomic Plants.

Authors:
Basharat Ali

Plants (Basel) 2019 Apr 4;8(4). Epub 2019 Apr 4.

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590, Pakistan.

The main objective of this study was to evaluate the genetic diversity and agricultural significance of bacterial communities associated with the surfaces of selected agronomic plants (carrot, cabbage and turnip). The bacterial diversity of fresh agricultural produce was targeted to identify beneficial plant microflora or opportunistic human pathogens that may be associated with the surfaces of plants. Bacterial strains were screened in vitro for auxin production, biofilm formation and antibiotic resistance. Read More

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https://www.mdpi.com/2223-7747/8/4/91
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http://dx.doi.org/10.3390/plants8040091DOI Listing
April 2019
2 Reads

A Novel Mutation in Chronic Granulomatous Disease: Treating the Family, Not Just the Patient.

Front Pediatr 2019 28;7:107. Epub 2019 Mar 28.

Division of Pediatric Allergy and Immunology, Department of Pediatrics, Penn State Milton S. Hershey Medical Center, Hershey, PA, United States.

Chronic Granulomatous Disease (CGD) is caused by genetic defects in the phagocyte NADPH oxidase leading to potentially severe infections with catalase positive micro-organisms. With the innate immune system being affected this disease usually presents before the age of 5 years with infections involving the skin, lung, liver or lymphnodes. Infections with specific catalase positive organisms, especially and prompt a workup for CGD in affected patients. Read More

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https://www.frontiersin.org/article/10.3389/fped.2019.00107/
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http://dx.doi.org/10.3389/fped.2019.00107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447646PMC
March 2019
3 Reads

Metal Resistance in Bacteria from Contaminated Arctic Sediment is Driven by Metal Local Inputs.

Arch Environ Contam Toxicol 2019 Apr 13. Epub 2019 Apr 13.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166, Messina, Italy.

Anthropogenic impact over the Pasvik River (Arctic Norway) is mainly caused by emissions from runoff from smelter and mine wastes, as well as by domestic sewage from the Russian, Norwegian, and Finnish settlements situated on its catchment area. In this study, sediment samples from sites within the Pasvik River area with different histories of metal input were analyzed for metal contamination and occurrence of metal-resistant bacteria in late spring and summer of 2014. The major differences in microbial and chemical parameters were mostly dependent on local inputs than seasonality. Read More

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http://link.springer.com/10.1007/s00244-019-00628-7
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http://dx.doi.org/10.1007/s00244-019-00628-7DOI Listing
April 2019
1 Read

Production optimization, characterization, and covalent immobilization of a thermophilic Serratia rubidaea lipase isolated from an Algerian oil waste.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sidi Mansour Road Km 6, P.O. Box 1177, Sfax, 3018, Tunisia.

A new thermophilic non-induced lipase producer named Serratia rubidaea strain Nehal-mou was isolated from oil waste in Tissemsilat, Algeria. The most influential lipase production parameters were screened by the Plackett-Burman design for enhancing enzyme yield. An optimum condition of a 1. Read More

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http://dx.doi.org/10.1007/s11033-019-04774-yDOI Listing

Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle .

Biotechnol Biofuels 2019 1;12:70. Epub 2019 Apr 1.

1Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, No. 778, Riverside Road, Central District, Leshan, 614000 Sichuan China.

Background: Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, , a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to date, no studies have evaluated the role of gut symbiotic microbiota of the snout beetle on bamboo lignocellulose degradation. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1411-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442426PMC
April 2019
2 Reads

Characteristics of the Intestinal Microbiota in Very Low Birth Weight Infants With Extrauterine Growth Restriction.

Front Pediatr 2019 26;7:99. Epub 2019 Mar 26.

Shenzhen Children's Hospital, Shenzhen, China.

Very low birth weight (VLBW) infants, which experience significant postnatal growth restriction at the time of discharge, are at high risk of later growth failure and long-term consequences. This study aims to characterize the structure of intestinal microbiome community in VLBW infants with extrauterine growth restriction (EUGR). Twenty-three VLBW infants appropriate for gestational age (GA) hospitalized at the neonatal intensive care unit of the BaoAn Maternal and Child Care Hospital (Shenzhen, China) were enrolled in this study, which were divided into the growth restriction group (EUGR; = 12) and the normal growth group (AGA; = 11). Read More

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https://www.frontiersin.org/article/10.3389/fped.2019.00099/
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http://dx.doi.org/10.3389/fped.2019.00099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443964PMC
March 2019
4 Reads

Mind the Coverage Gap: A PK-PD Evaluation of Ertapenem for Patients with Hospital-Acquired or Ventilator-Associated Bacterial Pneumonia.

Antimicrob Agents Chemother 2019 Apr 8. Epub 2019 Apr 8.

Institute for Clinical Pharmacodynamics, Inc., Schenectady, NY, USA.

Ertapenem provides activity against many pathogens commonly associated with hospital-acquired and ventilator-associated bacterial pneumoniae (HABP and VABP, respectively), including methicillin-susceptible and numerous Gram-negative pathogens with one major gap in coverage - Pharmacokinetic-pharmacodynamic (PK-PD) target attainment analyses were conducted to evaluate ertapenem against the most prevalent Enterobacteriaceae causing HABP/VABP. The objective of these analyses was to provide dose selection support for and demonstrate the appropriateness of ertapenem to empirically treat patients with HABP/VABP when administered with murepavadin, a novel targeted antimicrobial exhibiting a highly specific spectrum of activity against A previously-developed population pharmacokinetic model, total-drug epithelial lining fluid to free-drug serum penetration ratio, contemporary surveillance data for ertapenem against Enterobacteriaceae, and %T>MIC targets associated with efficacy were used to conduct Monte Carlo simulations for five ertapenem regimens administered over short or prolonged durations of infusion. Overall total-drug ELF percent probabilities of PK-PD target attainment based on a %T>MIC target of 35% among simulated patients with HABP/VABP arising from Enterobacteriaceae based on pathogen prevalence data for nosocomial pneumonia ranged from 89. Read More

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http://aac.asm.org/lookup/doi/10.1128/AAC.00318-19
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http://dx.doi.org/10.1128/AAC.00318-19DOI Listing
April 2019
2 Reads

A survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries.

Microbiologyopen 2019 Apr 4:e839. Epub 2019 Apr 4.

Division of Microbiology, Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland.

In recent years, the rise in antimicrobial resistance (AR) in the healthcare setting as well as the environment has been recognized as a growing public health problem. The Chesapeake Bay (CB) and its upper tributaries (UT) is a large and biologically diverse estuary. This pilot study evaluated the presence of AR of gram-negative bacteria isolated from water samples collected at various sites of the Chesapeake Bay. Read More

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http://dx.doi.org/10.1002/mbo3.839DOI Listing

Draft Genome Sequences of 38 Serratia marcescens Isolates Associated with Acroporid Serratiosis.

Microbiol Resour Announc 2019 Apr 4;8(14). Epub 2019 Apr 4.

Department of Life Sciences, Texas A&M University at Corpus Christi, Corpus Christi, Texas, USA

is a Gram-negative bacterium causally linked to acroporid serratiosis, a form of white pox disease implicated in the decline of elkhorn corals. We report draft genomes of 38 isolates collected from host and nonhost sources. The availability of these genomes will aid future analyses of acroporid serratiosis. Read More

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http://dx.doi.org/10.1128/MRA.00194-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449557PMC
April 2019
1 Read

Quorum sensing inimical activity of against gram negative bacterial pathogens by signalling interference.

3 Biotech 2019 Apr 1;9(4):163. Epub 2019 Apr 1.

1Bioresource Technology Lab, Department of Biotechnology, Thiruvalluvar University, Vellore, TN India.

Bacterial virulence is controlled by a cascade of genes influenced by quorum sensing alias bacterial signalling.The present study was intended to develop an effective module that could constrain bacterial communication without harming the host. Quorum quenching ability of was screened upon chromogenic reporter strains such as , and . Read More

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http://dx.doi.org/10.1007/s13205-019-1695-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443769PMC

Prodigiosin isolated from Serratia marcescens in the Periplaneta americana gut and its apoptosis‑inducing activity in HeLa cells.

Oncol Rep 2019 Jun 2;41(6):3377-3385. Epub 2019 Apr 2.

School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, P.R. China.

Serratia marcescens are considered to be abundant and optimal resources for obtaining prodigiosin, which can be isolated from soil, water, plants and air but rarely from insects. In the present study, a strain of Serratia marcescens named WA12‑1‑18 was isolated from the gut of Periplaneta americana, which was capable of producing high levels of pigment reaching 2.77 g/l via solid fermentation and was identified as prodigiosin by ultraviolet, high performance liquid chromatography (LC), Fourier‑transform infrared spectroscopy, LC‑mass spectroscopy and nuclear magnetic resonance. Read More

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http://dx.doi.org/10.3892/or.2019.7089DOI Listing
June 2019
2 Reads

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms.

MBio 2019 04 2;10(2). Epub 2019 Apr 2.

Center for Medical Mycology, Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA

Dysbiosis of the gut microbiome has been implicated in inflammatory bowel diseases. We have shown that levels of , along with those of and , are significantly elevated in Crohn's disease (CD) patients. Here, we evaluated the ability of a novel probiotic to prevent and treat polymicrobial biofilms (PMB) formed by with and Since has been reported to be elevated in CD patients, we investigated the interactions of with these bacterial species in biofilm formation. Read More

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http://dx.doi.org/10.1128/mBio.00338-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456750PMC
April 2019
3 Reads

The composition and abundance of bacterial communities residing in the gut of Glossina palpalis palpalis captured in two sites of southern Cameroon.

Parasit Vectors 2019 Apr 2;12(1):151. Epub 2019 Apr 2.

INTERTRYP, Institut de Recherche pour le Développement, University of Montpellier, Montpellier, France.

Background: A number of reports have demonstrated the role of insect bacterial flora on their host's physiology and metabolism. The tsetse host and vector of trypanosomes responsible for human sleeping sickness (human African trypanosomiasis, HAT) and nagana in animals (African animal trypanosomiasis, AAT) carry bacteria that influence its diet and immune processes. However, the mechanisms involved in these processes remain poorly documented. Read More

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http://dx.doi.org/10.1186/s13071-019-3402-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444424PMC
April 2019
3 Reads

Pyrosequencing Uncovers a Shift in Bacterial Communities Across Life Stages of (Coleoptera: Chrysomelidae).

Front Microbiol 2019 12;10:466. Epub 2019 Mar 12.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.

Bacterial symbionts of insects affect a wide array of host traits including fitness and immunity. (Maulik), commonly known as hispid leaf beetle is a destructive palm pest around the world. Understanding the dynamics of microbiota is essential to unravel the complex interplay between and its bacterial symbionts. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424052PMC
March 2019
1 Read

Severe and Progressive Cellulitis Caused by Serratia marcescens Following a Dog Scratch.

J Investig Med High Impact Case Rep 2019 Jan-Dec;7:2324709619832330

1 Medical College of Georgia, Augusta University, Augusta, GA, USA.

Soft tissue infections occur in over 30% of patients with chemotherapy-induced neutropenia. Gram-positive bacterial infections predominate early in neutropenia, and likelihood of infection by resistant bacteria and fungi increases with prolonged neutropenia. Prior infections and exposures influence the risk of rare pathogens. Read More

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http://dx.doi.org/10.1177/2324709619832330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444764PMC
April 2019
2 Reads

Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle.

Ann Agric Environ Med 2019 Mar 21;26(1):39-45. Epub 2018 Feb 21.

Department of Microbiology, Nicolaus Copernicus University, Toruń, Poland; Collegium Medicum of L. Rydygier, Bydgoszcz, Poland.

Introduction: Mastitis may result in physical, chemical and microbiological changes in milk and pathological lesions in the glandular tissue. Milk derived from cows with mastitis may become a cause of infections in humansw and animals.

Objectives: The aim of this study was to assess the effectiveness of selected dipping agents in the inactivation of several bacteria that may cause mastitis in cattle. Read More

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http://dx.doi.org/10.26444/aaem/82626DOI Listing

Efficient removal of Pb(II) from aqueous solution by a novel ion imprinted magnetic biosorbent: Adsorption kinetics and mechanisms.

PLoS One 2019 27;14(3):e0213377. Epub 2019 Mar 27.

Xiang Ya School of Public Health, Central South University, Changsha,Hunan, China.

It is vital to understand the adsorption mechanisms and identify the adsorption kinetics when applying an adsorbent to remove heavy metals from aqueous solution. A Pb(II) imprinted magnetic biosorbent (Pb(II)-IMB) was developed for the removal of Pb2+ via lead ion imprinting technology and crosslinking reactions among chitosan (CTS), Serratia marcescens and Fe3O4. The effect of different parameters such as solution pH, adsorbent dosage, selectivity sorption and desorption were investigated on the absorption of lead ion by Pb(II)-IMB. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0213377PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437713PMC
March 2019
1 Read

How Hosts Taxonomy, Trophy, and Endosymbionts Shape Microbiome Diversity in Beetles.

Microb Ecol 2019 Mar 27. Epub 2019 Mar 27.

Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Krakow, Poland.

Bacterial communities play a crucial role in the biology, ecology, and evolution of multicellular organisms. In this research, the microbiome of 24 selected beetle species representing five families (Carabidae, Staphylinidae, Curculionidae, Chrysomelidae, Scarabaeidae) and three trophic guilds (carnivorous, herbivorous, detrivorous) was examined using 16S rDNA sequencing on the Illumina platform. The aim of the study was to compare diversity within and among species on various levels of organization, including evaluation of the impact of endosymbiotic bacteria. Read More

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http://dx.doi.org/10.1007/s00248-019-01358-yDOI Listing

Detection and geographic distribution of seven facultative endosymbionts in two Rhopalosiphum aphid species.

Microbiologyopen 2019 Mar 25:e00817. Epub 2019 Mar 25.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Study of the mutualistic associations between facultative symbionts and aphids are developed only in a few models. That survey on the situation and distribution of the symbionts in a certain area is helpful to obtain clues for the acquisition and spread of them as well as their roles played in host evolution. To understand the infection patterns of seven facultative symbionts (Serratia symbiotica, Hamiltonella defensa, Regiella insecticola, Rickettsia, Spiroplasma, Wolbachia, and Arsenophonus) in Rhopalosiphum padi (Linnaeus) and Rhopalosiphum maidis (Fitch), we collected 882 R. Read More

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http://dx.doi.org/10.1002/mbo3.817DOI Listing

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds.

J Sci Food Agric 2019 Mar 24. Epub 2019 Mar 24.

Área de Microbiología. Departamento de Ciencias de la Salud. Facultad de Ciencias Experimentales, Universidad de Jaén, 23071, Jaén, Spain.

Background: Heavy metal pollution may act as persistent selective pressure that favours the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal tolerant bacteria from soils in olive tree fields routinely treated with copper derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics.

Results: 595 bacterial isolates from 45 olive tree agricultural fields were studied. Read More

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http://dx.doi.org/10.1002/jsfa.9708DOI Listing

Rapid detection of coliform bacteria using a lateral flow test strip assay.

Authors:
Tatsuya Tominaga

J Microbiol Methods 2019 Mar 20;160:29-35. Epub 2019 Mar 20.

Saitama Industrial Technology Center North Institute, 2-133, Suehiro, Kumagayashi, Saitama 360-0031, Japan. Electronic address:

Coliform bacteria in foods are enumerated at food processing plants and are used as sanitary and quality indicators. To detect coliform bacteria rapidly, seven Lateral Flow Test Strips (LFTSs) that can detect the genera Aeromonas, Citrobacter, Enterobacter, Hafnia, Klebsiella/Raoultella, Pantoea and Serratia were developed. For 55 tested food isolates, the detection rate of each individual LFTS assay was only 38% to 76%, but the detection rate of the 7 combined assays was 100%. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01677012193008
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http://dx.doi.org/10.1016/j.mimet.2019.03.013DOI Listing
March 2019
2 Reads

Gut microbiota specific signatures are related to the successful rate of bariatric surgery.

Am J Transl Res 2019 15;11(2):942-952. Epub 2019 Feb 15.

Unidad de Gestión Clínica de Endocrinología y Nutrición del Hospital Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga (IBIMA), Universidad de Málaga Málaga, Spain.

Bariatric surgery (BS) success rates vary in the long-time. A better understanding of weight-loss response may help improve the outcomes of BS. Gut microbiome could be implicated in the successful rate of BS. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413284PMC
February 2019
2 Reads

Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10.

Sci Rep 2019 Mar 19;9(1):4854. Epub 2019 Mar 19.

Department of Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan.

We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Read More

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http://www.nature.com/articles/s41598-019-41237-7
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http://dx.doi.org/10.1038/s41598-019-41237-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425002PMC
March 2019
2 Reads

Epigenetic Mechanisms Are Involved in Sex-Specific -Generational Immune Priming in the Lepidopteran Model Host .

Front Physiol 2019 4;10:137. Epub 2019 Mar 4.

Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany.

Parents invest in their offspring by transmitting acquired resistance against pathogens that only the parents have encountered, a phenomenon known as -generational immune priming (TGIP). Examples of TGIP are widespread in the animal kingdom. Female vertebrates achieve TGIP by passing antibodies to their offspring, but the mechanisms of sex-specific TGIP in invertebrates are unclear despite increasing evidence suggesting that both male-specific and female-specific TGIP occurs in insects. Read More

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http://dx.doi.org/10.3389/fphys.2019.00137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410660PMC

Monitoring of volatile production in cooked poultry products using selected ion flow tube-mass spectrometry.

Food Res Int 2019 May 24;119:196-206. Epub 2019 Jan 24.

Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.

Cooked poultry products are nutritious and economically valuable products that are at risk of bacterial spoilage, which can be postponed by cooling and modified-atmosphere-packaging (MAP). In this study, a cooked chicken product was stored at three different temperature ranges (4-6 °C, 7-9 °C, and 11-13 °C) and volatile production was measured over time using selected ion flow tube-mass spectrometry (SIFT-MS). The identities of the volatiles formed were confirmed by headspace solid-phase microextraction coupled with gas chromatography time-of-flight mass spectrometry (HS-SPME-GC-TOF-MS) analysis. Read More

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http://dx.doi.org/10.1016/j.foodres.2019.01.063DOI Listing

Serratia sp. CP-13 augments the growth of cadmium (Cd) stressed Linum usitatissimum L. by limited Cd uptake, enhanced nutrient acquisition and anti-oxidative potential.

J Appl Microbiol 2019 Mar 18. Epub 2019 Mar 18.

Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91Stockholm, Sweden.

Aims: The current study was aimed to evaluate the beneficial effects and bioremediation potential of a Cd-tolerant bacterial strain, Serratia sp. CP-13, on the physiological and biochemical functions of Linum usitatissimum L., under Cd stress. Read More

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http://dx.doi.org/10.1111/jam.14252DOI Listing

Sub-inhibitory concentration of ertapenem induces overexpression of regulator of antibiotic resistance A in .

Indian J Med Microbiol 2018 Oct-Dec;36(4):569-571

Department of Microbiology, Assam University, Silchar, Assam, India.

AcrAB-TolC is a tripartite efflux pump system constitutively expressed which functions as an intrinsic-resistant mechanism found to be responsible for conferring resistance towards dyes, detergents and different compounds including various classes of antibiotics. One global regulator belonging to AraC-type regulator family, regulator of antibiotic resistance A (RarA) up-regulates the expression of AcrAB-TolC encoded in Klebsiella pneumoniae, Enterobacter sp. 638, Serratia proteamaculans 568 and Enterobacter cloacae resulting in multidrug-resistant phenotypes. Read More

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http://dx.doi.org/10.4103/ijmm.IJMM_18_436DOI Listing

Phylogenetic Studies on the Prodigiosin Biosynthetic Operon.

Curr Microbiol 2019 May 16;76(5):597-606. Epub 2019 Mar 16.

Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, 600 025, India.

Prodigiosin and undecylprodigiosin are tripyrrolic red pigmented antibiotics produced by certain bacteria. Many strains of Serratia and certain other Gammaproteobacteria produce prodigiosin and undecylprodigiosin is produced by certain strains of Streptomyces. This is a multistage process which involves the synthesis of a bipyrrolic compound from L-proline and its subsequent condensation with a mono pyrrole synthesized from 2-octenal in the case of prodigiosin and malonyl-CoA in the case of undecylprodigiosin respectively. Read More

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http://dx.doi.org/10.1007/s00284-019-01665-0DOI Listing

Identification of Pathogenic Bacteria from Public Libraries via Proteomics Analysis.

Int J Environ Res Public Health 2019 Mar 14;16(6). Epub 2019 Mar 14.

Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.

Hazardous organisms may thrive on surfaces that are often exposed to human contact, including children's library books. In this study, swab samples were taken from 42 children's books collected from four public libraries in Texas and California. Samples were then cultivated in brain⁻heart infusion (BHI) medium and then in Luria broth (LB) medium containing either ampicillin or kanamycin. Read More

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http://dx.doi.org/10.3390/ijerph16060912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466425PMC
March 2019
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Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.

Sci Rep 2019 Mar 12;9(1):4270. Epub 2019 Mar 12.

School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.

In the present study, genomic analysis of a previously reported carbon dioxide (CO) sequestering bacterium Serratia sp. ISTD04 was performed along with exopolysaccharide (EPS) production. Genomic analysis identified key and accessory enzymes responsible for CO sequestration. Read More

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http://dx.doi.org/10.1038/s41598-019-41052-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414628PMC
March 2019
1 Read
5.078 Impact Factor

-acyl-homoserine lactone quorum sensing switching from acidogenesis to solventogenesis during the fermentation process in MG1.

J Microbiol Biotechnol 2019 Feb 28. Epub 2019 Feb 28.

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.

-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by MG1 and its isogenic with or without supplementing exogenous -hexanoyl-L-homoserine lactone (C-HSL) were systematically investigated. The results showed that disruption resulted in rapid pH drops from 7. Read More

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http://dx.doi.org/10.4014/jmb.1810.10026DOI Listing
February 2019

Fluoranthene Biodegradation by Serratia sp. AC-11 Immobilized into Chitosan Beads.

Appl Biochem Biotechnol 2019 Mar 11. Epub 2019 Mar 11.

Laboratory of Natural Organic Matter, Department of Chemistry, Federal University of Sergipe, Av. Marechal Rondon, Jardim Rosa Elze, Sao Cristovao, SE, 49100-000, Brazil.

The intensive production of polycyclic aromatic hydrocarbons by anthropogenic activities is a serious environmental problem. Therefore, new bioremediation methods are required to avoid widespread contamination. In this work, Serratia sp. Read More

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http://dx.doi.org/10.1007/s12010-019-02980-9DOI Listing

Infection pattern and negative effects of a facultative endosymbiont on its insect host are environment-dependent.

Sci Rep 2019 Mar 8;9(1):4013. Epub 2019 Mar 8.

Department of Entomology, Nanjing Agricultural University, Nanjing, 210095, China.

Regiella insecticola is a bacterial endosymbiont in insects that exhibits a negative effect on the fitness of hosts. Thus, it is not clear why this costly endosymbiont can persist in host populations. Here, we tested a hypothesis that the infection pattern and negative roles of the endosymbiont were not constant but environmentally dependent. Read More

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http://dx.doi.org/10.1038/s41598-019-40607-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408509PMC

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection.

Sci Rep 2019 Mar 8;9(1):4012. Epub 2019 Mar 8.

Institute of Systematics and Ecology of Animals, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630091, Russia.

Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation by the ectoparasitoid Habrobracon hebetor. In addition, spontaneous bacterioses occurred more often in envenomated larvae. Read More

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http://dx.doi.org/10.1038/s41598-019-40301-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408550PMC
March 2019
2 Reads

New Insights into the Nature of Symbiotic Associations in Aphids: Infection Process, Biological Effects and Transmission Mode of Cultivable Bacteria.

Appl Environ Microbiol 2019 Mar 8. Epub 2019 Mar 8.

Earth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, 1348, Louvain-la-Neuve, Belgium.

Symbiotic microorganisms are widespread in nature and can play a major role in the ecology and evolution of animals. The aphid- bacterium interaction provides a valuable model to study mechanisms behind these symbiotic associations. The recent discovery of cultivable strains having the possibility of free-living lifestyle allowed us to simulate their environmental acquisition by aphids to examine the mechanisms involved in this infection pathway. Read More

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http://dx.doi.org/10.1128/AEM.02445-18DOI Listing
March 2019
1 Read

Symbiont-mediated chemical defense in the invasive ladybird .

Ecol Evol 2019 Feb 25;9(4):1715-1729. Epub 2019 Jan 25.

Institute for Insect Biotechnology Justus-Liebig-University of Giessen Giessen Germany.

The volatile alkylpyrazines methyl- and methoxypyrazines (MPs) present in the reflex bleeds of coccinellid beetles such as the harlequin ladybird beetle are important semiochemicals that function in antipredatory defense behavior. Pyrazines have also been coadapted from a primarily defensive role into pheromones that function in intraspecific communication, attraction, and aggregation behavior. However, the biosynthesis of MPs in ladybird beetles is poorly understood. Read More

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http://dx.doi.org/10.1002/ece3.4840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392489PMC
February 2019
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Recombinant Expression of Outer Membrane Phospholipase A (A1) in and Immobilization With Graphene Oxide-Based FeO Nanoparticles for Rapeseed Oil Degumming.

Front Microbiol 2019 21;10:334. Epub 2019 Feb 21.

Anhui Key Laboratory of Intensive Processing of Agricultural Products, College of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Enzymatic degumming is an effective approach to produce nutritional, safe, and healthy refined oil. However, the high cost and low efficiency of phospholipase limit the application of enzymatic degumming. In this study, an 879 bp outer membrane phospholipase A (A1) (OM-PLA1) gene encoding 292 amino acid residues was isolated from the genome of . Read More

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http://dx.doi.org/10.3389/fmicb.2019.00334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393389PMC
February 2019
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AmpC β-lactamase-producing Enterobacterales: what a clinician should know.

Infection 2019 Mar 6. Epub 2019 Mar 6.

Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.

Background: Enterobacterales are among the most common causes of bacterial infections in the community and among hospitalized patients, and multidrug-resistant (MDR) strains have emerged as a major threat to human health. Resistance to third-generation cephalosporins is typical of MDRs, being mainly due to the production of extended spectrum β-lactamases or AmpC-type β-lactamases.

Objective: The objective of this paper is to review the epidemiological impact, diagnostic issues and treatment options with AmpC producers. Read More

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http://dx.doi.org/10.1007/s15010-019-01291-9DOI Listing
March 2019
14 Reads

Distribution and Genetic Diversity of Genes Involved in Quorum Sensing and Prodigiosin Biosynthesis in the Complete Genome Sequences of Serratia marcescens.

Genome Biol Evol 2019 03;11(3):931-936

Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, Japan.

Quorum sensing is a cell density-dependent regulation of gene expression. N-acyl-l-homoserine lactone (AHL) is a major quorum-sensing signaling molecule in gram-negative bacteria and synthesized by the LuxI family protein. The genus Serratia is known as a producer of the red pigment, prodigiosin, whose biosynthesis is dependent on the pig gene cluster. Read More

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http://dx.doi.org/10.1093/gbe/evz046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433178PMC

Whole-genome-based analysis reveals multiclone Serratia marcescens outbreaks in a non-Neonatal Intensive Care Unit setting in a tertiary care hospital in India.

J Med Microbiol 2019 Apr 6;68(4):616-621. Epub 2019 Mar 6.

3 Department of Periodontology and Restorative Dentistry, Muenster University Hospital, Waldeyerstrasse 30, 48149 Muenster, Germany.

We report the use of next generation sequencing (NGS) for investigating an outbreak of 13 cases of Serratia marcescens blood stream infections in a non-Neonatal Intensive Care Unit (non-NICU) setting in a tertiary care hospital in India over 5 months. Thirteen cases of sepsis due to S. marcescens were identified in various Intensive Care Units (ICUs) over 5 months. Read More

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http://dx.doi.org/10.1099/jmm.0.000947DOI Listing
April 2019
2 Reads
2.248 Impact Factor

A Primer on AmpC Beta-Lactamases: Necessary Knowledge for an Increasingly Multidrug-Resistant World.

Clin Infect Dis 2019 Mar 6. Epub 2019 Mar 6.

Department of Pathology, Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, MD.

Understanding the nuances of AmpC β-lactamase mediated resistance can be challenging - even for the infectious diseases specialist. AmpC resistance can be classified into three categories: (a) inducible chromosomal resistance that emerges in the setting of a β-lactam compound, (b) stable derepression due to mutations in ampC regulatory genes, or (c) the presence of plasmid-mediated ampC genes. This review will mainly focus on inducible AmpC resistance in Enterobacteriaceae. Read More

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http://dx.doi.org/10.1093/cid/ciz173DOI Listing

Extending the 30-minute rule for red cell units - investigation of the bacterial risk of 60-minute exposures to ambient temperature.

Vox Sang 2019 Apr 4;114(3):189-197. Epub 2019 Mar 4.

National Bacteriology Laboratory, NHS Blood and Transplant, London, UK.

Background And Objectives: In the UK, a significant proportion of red cell units is discarded due to the 30-min rule governing out of temperature control. Studies have shown that repeated warming to ambient temperature has little impact on red cell quality or bacterial growth. We aimed to validate extension of the rule to 60 minutes by investigation of repeated same, and different, day exposures on bacterial growth. Read More

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http://dx.doi.org/10.1111/vox.12747DOI Listing
April 2019
5 Reads