11,235 results match your criteria Serratia


qPCR quantification of Carnobacterium maltaromaticum, Brochothrix thermosphacta, and Serratia liquefaciens growth kinetics in mixed culture.

J Microbiol Methods 2020 May 29:105961. Epub 2020 May 29.

Tasmanian Institute of Agriculture, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia. Electronic address:

Quantifying growth kinetics of specific spoilage microorganisms in mixed culture is required to describe the evolution of food microbiomes. A qPCR method was developed to selectively amplify individual meat spoilage bacteria, Carnobacterium maltaromaticum, Brochothrix thermosphacta and Serratia liquefaciens, within a broth medium designed to simulate the composition of beef. An optimized method of DNA extraction was produced for standard curve construction. Read More

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http://dx.doi.org/10.1016/j.mimet.2020.105961DOI Listing

Altered Gut Microbiota and Immunity Defines Survival in .

Front Immunol 2020 14;11:609. Epub 2020 May 14.

Laboratory of Host-Parasite Interaction Studies, ICMR-National Institute of Malaria Research, New Delhi, India.

Blood-feeding enriched gut-microbiota boosts mosquitoes' anti- immunity. Here, we ask how alters gut-microbiota, anti- immunity, and impacts tripartite -mosquito-microbiota interactions in the gut lumen. We used a metagenomics and RNAseq strategy to address these questions. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240202PMC

Short communication: Effects of moringa extract on adhesion and invasion of Escherichia coli O55 in bovine mammary epithelial cells.

J Dairy Sci 2020 May 28. Epub 2020 May 28.

Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea. Electronic address:

The objective of this study was to evaluate the antibacterial activities of extract derived from moringa leaves. In particular, the effect of moringa extract (Mor) on adhesion and invasion of Escherichia coli O55, Enterococcus faecalis, Staphylococcus simulans, and Serratia liquefaciens was evaluated in bovine mammary epithelial cells (MAC-T). Broth microdilution method, minimum inhibitory concentration and minimum bactericidal concentration assays, adhesion and invasion assays, and real-time PCR were performed. Read More

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http://dx.doi.org/10.3168/jds.2019-17774DOI Listing

Chitinase system of , and characterization of enzymes involved in chitin degradation.

Biosci Biotechnol Biochem 2020 May 29:1-12. Epub 2020 May 29.

Graduate School of Science and Technology, Niigata University , Niigata, Japan.

The genes encoding chitin-degrading enzymes in SWSY-1.411 were identified and cloned in . The strain contained two glycoside hydrolase (GH) families 18 chitinases: AsChiA and AsChiB, two GH19 chitinases: AsChiC and AsChiD, and an auxiliary activities family 10 protein, lytic polysaccharide monooxygenase: AsLPMO10A. Read More

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http://dx.doi.org/10.1080/09168451.2020.1771539DOI Listing

The Hypopiezotolerant Bacterium, , Failed to Grow in Mars Analog Soils under Simulated Martian Conditions at 7 hPa.

Life (Basel) 2020 May 26;10(6). Epub 2020 May 26.

Space Life Sciences Laboratory, Department of Plant Pathology, University of Florida, 505 Odyssey Way, Exploration Park, Merritt Island, FL 32953, USA.

The search for life on Mars is predicated on the idea that Earth and Mars life (if present) should be both carbon- and water-based with similar forms of evolution. However, the astrobiology community can currently only investigate plausible Martian microbial ecosystems by using Terran life-forms as proxies. In order to examine how life might persist on Mars, we used a hypopiezotolerant bacterium (def. Read More

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http://dx.doi.org/10.3390/life10060077DOI Listing

Genetically modified entomopathogenic bacteria, recent developments, benefits and impacts: A review.

Sci Total Environ 2020 May 11;734:139169. Epub 2020 May 11.

Department of Crop and Animal Production, Safiye Cikrikcioglu Vocational College, Kayseri University, Kayseri, Turkey.

Entomopathogenic bacteria (EPBs), insect pathogens that produce pest-specific toxins, are environmentally-friendly alternatives to chemical insecticides. However, the most important problem with EPBs application is their limited field stability. Moreover, environmental factors such as solar radiation, leaf temperature, and vapor pressure can affect the pathogenicity of these pathogens and their toxins. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139169DOI Listing

iTRAQ-Based Comparative Proteomic Analysis of Larval Midgut From the Beet Armyworm, (Hübner) (Lepidoptera: Noctuidae) Challenged With the Entomopathogenic Bacteria .

Front Physiol 2020 8;11:442. Epub 2020 May 8.

Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China.

Entomopathogenic bacteria is widely used as an environmentally friendly biocontrol agent against various pests, including . Understanding the immune defense mechanism of through comparative proteomic analysis can identify the key proteins expressed in response to the microbial infection. Here, we employed the as isobaric tags for relative and absolute quantification (iTRAQ) technique to investigate the effects of on the proteomic expression of Based on the molecular functional analysis, the differentially expressed proteins (DEPs) were mainly involved in the binding process and catalytic activities. Read More

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http://dx.doi.org/10.3389/fphys.2020.00442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227483PMC

Long-lasting nosocomial persistence of chlorhexidine-resistant Serratia marcescens in a veterinary hospital.

Vet Microbiol 2020 Jun 15;245:108686. Epub 2020 Apr 15.

Unité Antibiorésistance et Virulence Bactériennes, Université de Lyon - ANSES, 31 avenue Tony Garnier, 69007 Lyon cedex, France. Electronic address:

Healthcare-associated infections (HAIs) are often overlooked in veterinary medicine. Serratia marcescens isolates were recovered over a ten-year period from companion animals in a French veterinary hospital. The pets were sampled either for diagnostic purposes or to monitor colonization. Read More

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http://dx.doi.org/10.1016/j.vetmic.2020.108686DOI Listing

In vitro conjugation kinetics of AmpC, broad spectrum and extended-spectrum beta-lactamase-producing Escherichia coli donors and various Enterobacteriaceae recipients.

BMC Microbiol 2020 May 25;20(1):133. Epub 2020 May 25.

Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise-Str. 49, 14195, Berlin, Germany.

Background: Extended spectrum beta-lactamase (ESBL)-producing enterobacteria pose a major hazard to public health. Due to the possibility of genetic transfer, ESBL genes might spread to pathogenic enterobacterial strains. Thus, information on possible genetic transfer between enterobacteria is of high interest. Read More

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http://dx.doi.org/10.1186/s12866-020-01787-7DOI Listing

Serratia marcescens outbreak in the intensive care unit during the COVID-19 pandemic: A paradoxical risk?

Med Mal Infect 2020 May 21. Epub 2020 May 21.

Department of Medical and Toxicological Critical Care, Lariboisière Hospital, University of Paris, Inserm UMRS-1144, Paris, France. Electronic address:

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http://dx.doi.org/10.1016/j.medmal.2020.05.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241335PMC

A new bacteria-free strategy induced by MaGal2 facilitates pinewood nematode escape immune response from its vector beetle.

Insect Sci 2020 May 22. Epub 2020 May 22.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Symbiotic microbes play a crucial role in regulating parasite-host interactions; however, the role of bacterial associates in parasite-host interactions requires elucidation. In this study, we showed that, instead of introducing numerous symbiotic bacteria, dispersal of fourth-stage juvenile (J ) pinewood nematodes (PWNs), Bursaphelenchus xylophilus, only introduced few bacteria to its vector beetle, Monochamus alternatus (Ma). J showed weak binding ability to five dominant bacteria species isolated from the beetles' pupal chamber. Read More

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http://dx.doi.org/10.1111/1744-7917.12823DOI Listing

Prevalence of Extended-Spectrum Beta-Lactamase-Producing Causing Bloodstream Infections in Cancer Patients from Southwest of Iran.

Infect Drug Resist 2020 6;13:1319-1326. Epub 2020 May 6.

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Introduction: This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran.

Materials And Methods: In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35-37ºC for 24 hours and then sub-cultured on routine microbiology culture media. Read More

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http://dx.doi.org/10.2147/IDR.S254357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212769PMC

Metallo-β-Lactamase and Extended-Spectrum-β-Lactamase Production by Strains [Letter].

Infect Drug Resist 2020 5;13:1295-1297. Epub 2020 May 5.

Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, India.

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http://dx.doi.org/10.2147/IDR.S257872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221414PMC

Application of novel bacterial consortium for biodegradation of aromatic amine 2-ABS using response surface methodology.

J Microbiol Methods 2020 May 17;174:105941. Epub 2020 May 17.

Department of Chemistry, COMSATS University Islamabad, Lahore Campus, Pakistan. Electronic address:

There is a strong need to develop purification methods for textile industrial wastewater containing toxic azo dyes. The reductive cleavage of azo dyes can be made by anaerobic bacteria, but the products of aromatic amines require an aerobic process. In this study a novel bacterial dye degrading consortium (DDC) of five isolated strains identified with 16S rRNA sequence: Proteus mirabilis (KR732288), Bacillus anthracis (KR732289), Enterobacter hormaechei (KR732290), Pseudomonas aeruginosa (KR732293) and Serratia rubidaea (KR732296) were used to aerobically decompose metabolite 2-aminobenxenesulfonic acid (2-ABS), as a model compound. Read More

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http://dx.doi.org/10.1016/j.mimet.2020.105941DOI Listing
May 2020
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Endophytic bacterial and fungal microbiota in different cultivars of cassava (Manihot esculenta Crantz).

J Microbiol 2020 May 18. Epub 2020 May 18.

Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, P. R. China.

Endophytes colonize tissues of healthy host plants and play a crucial role in plant growth and development. However, little attention has been paid to the endophytes of tuber crops such as cassava, which is used as a staple food by approximately 800 million people worldwide. This study aimed to elucidate the diversity and composition of endophytic bacterial and fungal communities in different cassava cultivars using high-throughput sequencing. Read More

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http://dx.doi.org/10.1007/s12275-020-9565-xDOI Listing

Multicenter, Observational Cohort Study Evaluating Third-Generation Cephalosporin Therapy for Bloodstream Infections Secondary to , , and Species.

Antibiotics (Basel) 2020 May 14;9(5). Epub 2020 May 14.

Department of Clinical Pharmacy and Outcomes Sciences, University of South Carolina College of Pharmacy, Columbia, SC 29208, USA.

Objectives: There is debate on whether the use of third-generation cephalosporins (3GC) increases the risk of clinical failure in bloodstream infections (BSIs) caused by chromosomally-mediated AmpC-producing Enterobacterales (CAE). This study evaluates the impact of definitive 3GC therapy versus other antibiotics on clinical outcomes in BSIs due to Enterobacter, Serratia, or Citrobacter species.

Methods: This multicenter, retrospective cohort study evaluated adult hospitalized patients with BSIs secondary to Enterobacter, Serratia, or Citrobacter species from 1 January 2006 to 1 September 2014. Read More

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http://dx.doi.org/10.3390/antibiotics9050254DOI Listing

Early signs of gut microbiome aging: biomarkers of inflammation, metabolism, and macromolecular damage in young adulthood.

J Gerontol A Biol Sci Med Sci 2020 May 18. Epub 2020 May 18.

Department of Epidemiology, The Gillings School of Global Public Health, UNC-Chapel Hill, Chapel Hill, NC, USA.

Emerging links between gut microbiota and diseases of aging point to possible shared immune, metabolic, and cellular damage mechanisms, operating long before diseases manifest. We conducted 16S rRNA sequencing of fecal samples collected from a subsample (n=668) of Add Health Wave V, a nationally representative longitudinal study of adults aged 32-42. An overlapping subsample (n=345) included whole blood RNA-seq. Read More

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http://dx.doi.org/10.1093/gerona/glaa122DOI Listing

Lipophilic Metabolites from Five-needle Pines, Pinus armandii and Pinus kwangtungensis, Exhibiting Antibacterial Activity.

Chem Biodivers 2020 May 15. Epub 2020 May 15.

Institute of Cytology and Genetics, Laboratory of Genetic Engineering, Acad. Lavrentyev Ave. 10, Novosibirsk, RUSSIAN FEDERATION.

Lipophilic extractive metabolites from needles and defoliated twigs of Pinus armandii and P. kwangtungensis were studied by GC-MS. Needles of P. Read More

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http://dx.doi.org/10.1002/cbdv.202000201DOI Listing

Whole-Genome Sequences of Three Plant Growth-Promoting Rhizobacteria Isolated from Solanum tuberosum L. Rhizosphere in Tanzania.

Microbiol Resour Announc 2020 May 14;9(20). Epub 2020 May 14.

Georg-August University of Göttingen, Institute of Microbiology and Genetics Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Göttingen, Germany.

We present here the complete genome sequences of plant growth-promoting sp. strain MPUS7, sp. strain NGAS9, and sp. Read More

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http://dx.doi.org/10.1128/MRA.00371-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225545PMC

Novel thiazolinyl-picolinamide based palladium(II) complex-impregnated urinary catheters quench the virulence and disintegrate the biofilms of uropathogens.

Biofouling 2020 Mar 13;36(3):351-367. Epub 2020 May 13.

Biofilm Biology Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA Deemed University, Tirumalaisamudram, Tamil Nadu, India.

and are prominent members belonging to the group of ESKAPE pathogens responsible for Urinary Tract Infections (UTI) and nosocomial infections. Both the pathogens regulate several virulence factors, including biofilm formation through quorum sensing (QS), an intercellular communication mechanism. The present study describes the anti-biofilm and QS quenching effect of thiazolinyl-picolinamide based palladium(II) complexes against and Palladium(II) complexes showed quorum sensing inhibitory potential in inhibiting swarming motility behaviour, pyocyanin production and other QS mediated virulence factors in both and . Read More

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http://dx.doi.org/10.1080/08927014.2020.1765159DOI Listing

Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation.

Environ Sci Pollut Res Int 2020 May 12. Epub 2020 May 12.

Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan, 316021, Zhejiang, China.

A biosurfactant (BS) is a surface-active metabolite that is secreted by microbial metabolism, and can be used as a substitute for chemically synthesized surfactants. The first and most critical step to the successful application of BSs is to isolate bacterial strains with strong BS-producing capabilities. In this study, a BS-producing Serratia marcescens ZCF25 was isolated from the sludge of an oil tanker. Read More

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http://dx.doi.org/10.1007/s11356-020-09006-6DOI Listing

A tri-modular bacterial enzyme combining hydrolytic activity with oxidative glycosidic bond cleavage efficiently degrades chitin.

J Biol Chem 2020 May 12. Epub 2020 May 12.

Chemistry, Biotechnology, and Food Science, The Norwegian University of Life Sciences, Norway.

Findings from recent studies have indicated that enzymes containing more than one catalytic domain may be particularly powerful in the degradation of recalcitrant polysaccharides such as chitin and cellulose. Some known multi-catalytic enzymes contain several glycoside hydrolase (GH) domains and one or more carbohydrate-binding modules (CBMs). Here, using bioinformatics and biochemical analyses, we identified an enzyme, 1381 from the actinobacterium , that uniquely combines two different polysaccharide-degrading activities. Read More

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http://dx.doi.org/10.1074/jbc.RA120.013040DOI Listing

Outbreak investigation of Serratia marcescens neurosurgical site infections associated with a contaminated shaving razors.

Antimicrob Resist Infect Control 2020 May 12;9(1):64. Epub 2020 May 12.

Center for Infection Control and Prevention, Seoul National University Hospital, Seoul, South Korea.

Background: Surgical site infection (SSI) is the most common healthcare-associated infection. We report an outbreak of neurosurgical site infections caused by Serratia marcescens after craniotomy in a tertiary care hospital.

Methods: Between August 6 and 21, 2018, five cases of early-onset SSI caused by S. Read More

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http://dx.doi.org/10.1186/s13756-020-00725-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216399PMC

The siderophore, serratiochelin, is necessary for full virulence during bloodstream infection.

Infect Immun 2020 May 11. Epub 2020 May 11.

University of Michigan Medical School, Department of Microbiology and Immunology, Ann Arbor, Michigan, USA

is a bacterium frequently found in the environment, but over the last several decades has evolved into a concerning clinical pathogen, causing fatal bacteremia. To establish these infections, pathogens require specific nutrients-one very limited but essential nutrient is iron. We sought to characterize the iron acquisition systems in isolate UMH9, which was recovered from a clinical bloodstream infection. Read More

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http://dx.doi.org/10.1128/IAI.00117-20DOI Listing

Genetic diversity and in vitro activity of ceftazidime/avibactam and aztreonam/avibactam against imipenem-resistant Enterobacteriaceae isolates in Southwest China: a single-centre study.

J Glob Antimicrob Resist 2020 May 5. Epub 2020 May 5.

Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Objectives: This study aims to investigate the molecular mechanisms of imipenem-resistant Enterobacteriaceae and assess the antimicrobial activities of ceftazidime/avibactam (CAZ/AVI) and aztreonam/avibactam (ATM/AVI) against the imipenem-resistant isolates in a tertiary hospital in China.

Methods: A total of 91 imipenem-resistant Enterobacteriaceae were collected and genes encoding carbapenemases, ESBLs, AmpCs, and porins were detected using PCR. MICs and susceptibility were determined using in-house-prepared broth microdilution panels and interpreted with CLSI breakpoints. Read More

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http://dx.doi.org/10.1016/j.jgar.2020.04.023DOI Listing

Complete Genome Sequence of Tetracycline-Resistant Serratia liquefaciens S1, Isolated from Mixed Greens, Obtained Using Illumina MiSeq and Oxford Nanopore MinION Sequencing.

Microbiol Resour Announc 2020 May 7;9(19). Epub 2020 May 7.

Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, Germany.

strain S1 was isolated from mixed greens and sequenced with short-read Illumina MiSeq technology and long-read MinION technology. Hybrid sequence assembly generated a complete single circular chromosome and two extrachromosomal contigs, which together encoded 5,098 proteins, 85 tRNAs, and 22 rRNAs. Read More

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http://dx.doi.org/10.1128/MRA.00156-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206483PMC

Prevalence of Asymptomatic Bacteriuria and Antibiotic Susceptibility Patterns of Bacterial Isolates among Cancer Patients and Healthy Blood Donors at the University of Gondar Specialized Hospital.

Int J Microbiol 2020 16;2020:3091564. Epub 2020 Apr 16.

Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, University of Gondar, Gondar, Ethiopia.

Background: Urinary tract infections are the common types of infections in the community and health care settings. Despite the widespread availability of antibiotics, urinary tract infection remains a worldwide therapeutic problem. It is a continuous and significant problem in cancer patients. Read More

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http://dx.doi.org/10.1155/2020/3091564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183528PMC

Ready-to-eat vegetable salads served in Nigerian restaurants: a potential source of multidrug-resistant bacteria.

J Appl Microbiol 2020 May 6. Epub 2020 May 6.

Department of Biological Sciences, College of Natural and Applied Sciences, Igbinedion University Okada, Edo State, Nigeria.

Aim: Public health promotion of fruit and vegetables as healthier sources of nutrition in Nigeria has led to an increase in consumption of fruit and vegetables, particularly in the form of ready-to-eat (RTE) salads. However, the continual association of drug-resistant bacteria with fruit and vegetables creates an additional challenge for consumer safety. Hence this study was carried out to determine the prevalence of multidrug-resistant bacteria (MRB) in, and characterization of MRB isolated from, RTE salads. Read More

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http://dx.doi.org/10.1111/jam.14693DOI Listing

Valorization of xylose-rich hydrolysate from rice straw, an agroresidue, through biosurfactant production by the soil bacterium Serratia nematodiphila.

Sci Total Environ 2020 Aug 25;729:138933. Epub 2020 Apr 25.

Hindustan Petroleum Green R&D Centre, KIADB Industrial Area, Tarabanahalli, Devanagonthi, Hoskote, Bengaluru 560067, India. Electronic address:

Biosurfactants, amphiphilic compounds that reduce interfacial tension in oil-aqueous mixtures, are used in the petroleum, pharmaceutical, food, and agriculture industries. Fermentative production of biosurfactants requires expensive sugar or lipid substrates. Lignocellulosic biomass is a relatively cheap and abundant agricultural residue that can be used as an alternative substrate. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.138933DOI Listing

Microbiological Characteristics of Carbapenem-Resistant Clinical Isolates Collected from County Hospitals.

Infect Drug Resist 2020 22;13:1163-1169. Epub 2020 Apr 22.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Objective: To investigate the molecular characteristics of carbapenem-resistant (CRE) from county hospitals in China.

Materials and methods: Forty-three sequential non-duplicate CRE strains (including 33 isolates, 4 isolates, 3 isolates, 1 , 1 and 1 ) were collected from 4 county hospitals and 2 municipal hospitals. Antimicrobial susceptibility testing was conducted by broth microdilution method, using 3-aminophenylboronic acid and EDTA and the modified carbapenem inactivation method (mCIM) to screen phenotype of carbapenemase. Read More

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http://dx.doi.org/10.2147/IDR.S248147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183775PMC

Screening of Heavy Metal-Immobilizing Bacteria and Its Effect on Reducing Cd and Pb Concentrations in Water Spinach ( Forsk.).

Int J Environ Res Public Health 2020 Apr 30;17(9). Epub 2020 Apr 30.

Collaborative Innovation of Water Security for the Water Source Region of Mid-line of the South-to-North Diversion Project of Henan Province, College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.

Microbial immobilization is considered as a novel and environmentally friendly technology that uses microbes to reduce heavy metals accumulation in plants. To explore microbial resources which are useful in these applications, three water spinach rhizosphere soils polluted by different levels of heavy metals (heavy pollution (CQ), medium pollution (JZ), and relative clean (NF)) were collected. The community composition of heavy metal-immobilizing bacteria in rhizosphere soils and its effects on reducing the Cd and Pb concentrations in water spinach were evaluated. Read More

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http://dx.doi.org/10.3390/ijerph17093122DOI Listing

Proteomic profiling of by high-resolution mass spectrometry.

Bioimpacts 2020 26;10(2):123-135. Epub 2020 Mar 26.

Institute of Bioinformatics, International Technology Park, Bangalore-560066, Karnataka, India.

an opportunistic human pathogen, is reported as an important cause of nosocomial infection and outbreaks. Although the genome of (ATCC 13880) was completely sequenced by 2014, there are no studies on the proteomic profile of the organism. The objective of the present study is to analyze the protein profile of (ATCC 13880) using a high resolution mass spectrometry (MS). Read More

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http://dx.doi.org/10.34172/bi.2020.15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186543PMC

Characterization of bacterial communities associated with the pinewood nematode insect vector Monochamus alternatus Hope and the host tree Pinus massoniana.

BMC Genomics 2020 May 1;21(1):337. Epub 2020 May 1.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350000, China.

Background: Monochamus alternatus Hope is one of the insect vectors of pinewood nematode (Bursaphelenchus xylophilus), which causes the destructive pine wilt disease. The microorganisms within the ecosystem, comprising plants, their environment, and insect vectors, form complex networks. This study presents a systematic analysis of the bacterial microbiota in the M. Read More

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http://dx.doi.org/10.1186/s12864-020-6718-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195709PMC

Whole-Genome Sequence of a Plant Growth-Promoting Strain, Serratia marcescens BTL07, Isolated from the Rhizoplane of Capsicum annuum L.

Microbiol Resour Announc 2020 Apr 30;9(18). Epub 2020 Apr 30.

Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh

strain BTL07, which has the ability to promote growth and suppress plant diseases, was isolated from the rhizoplane of a chili plant. The draft genome sequence data of the strain will contribute to advancing our understanding of the molecular mechanisms underlying plant growth promotion and tolerance to different stresses. Read More

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http://dx.doi.org/10.1128/MRA.01484-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193939PMC

Genome Sequence of Resistant sp. Strain HRI, Isolated from a Bottle of Didecyldimethylammonium Chloride-Based Disinfectant.

Microbiol Resour Announc 2020 Apr 30;9(18). Epub 2020 Apr 30.

Department of Biochemical, Microbial and Food Biotechnology, University of the Free State, Bloemfontein, South Africa

Antimicrobial resistance is a significant issue, and it threatens the prevention and effective treatment of a range of bacterial infections. Here, we report the whole-genome sequence of the multidrug-resistant isolate sp. strain HRI. Read More

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http://dx.doi.org/10.1128/MRA.00095-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193921PMC

High variability of plasmid uptake rates in Escherichia coli isolated from sewage and river sediments.

PLoS One 2020 30;15(4):e0232130. Epub 2020 Apr 30.

Institute of Hydrobiology, TU Dresden, Dresden, Germany.

The horizontal transfer of plasmids is a key mechanism behind the spread of antibiotic resistance in bacteria. So far, transfer rate constants were measured for a variety of plasmids, donors and recipients. The employed strains typically had a long history in laboratories. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232130PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192377PMC

The FloR master regulator controls flotation, virulence and antibiotic production in Serratia sp. ATCC 39006.

Environ Microbiol 2020 Apr 30. Epub 2020 Apr 30.

Department of Biochemistry, University of Cambridge, Hopkins Building, Downing Site, Cambridge, CB2 1QW, UK.

Serratia sp. ATCC 39006 produces intracellular gas vesicles to enable upward flotation in water columns. It also uses flagellar rotation to swim through liquid and swarm across semi-solid surfaces. Read More

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http://dx.doi.org/10.1111/1462-2920.15048DOI Listing

Occurrence, Phenotypic and Molecular Characterization of Extended-Spectrum- and AmpC- β-Lactamase Producing Enterobacteriaceae Isolated From Selected Commercial Spinach Supply Chains in South Africa.

Front Microbiol 2020 15;11:638. Epub 2020 Apr 15.

Department of Plant and Soil Sciences, University of Pretoria, Pretoria, South Africa.

The increasing occurrence of multidrug-resistant (MDR) extended-spectrum β-lactamase- (ESBL) and/or AmpC β-lactamase-producing Enterobacteriaceae in health care systems, the environment and fresh produce is a serious concern globally. Production practices, processing and subsequent consumption of contaminated raw fruit and vegetables represent a possible human transmission route. The purpose of this study was to determine the presence of ESBL/AmpC-producing Enterobacteriaceae in complete spinach supply chains and to characterize the isolated strains phenotypically (antimicrobial resistance profiles) and genotypically (ESBL/AmpC genetic determinants, detection of class 1, 2, and 3 integrons). Read More

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http://dx.doi.org/10.3389/fmicb.2020.00638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176360PMC

Multiple Factors Determine the Structure of Bacterial Communities Associated With Under Artificial Rearing Conditions.

Front Microbiol 2020 15;11:605. Epub 2020 Apr 15.

Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria.

Insect symbionts are major manipulators of host's behavior. Their effect on parameters such as fecundity, male mating competitiveness, and biological quality in general, can have a major influence on the effectiveness of the sterile insect technique (SIT). SIT is currently being developed and applied against human disease vectors, including , as an environment-friendly method of population suppression, therefore there is a renewed interest on both the characterization of gut microbiota and their exploitation in artificial rearing. Read More

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http://dx.doi.org/10.3389/fmicb.2020.00605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176356PMC

Effects of oil substrate supplementation on production of prodigiosin by Serratia nematodiphila for dye-sensitized solar cell.

J Biotechnol 2020 Jun 27;317:16-26. Epub 2020 Apr 27.

Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai, Johor, 81310, Malaysia; Institute of Bioproduct Development, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.

Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterization of the red pigment produced by the bacteria has confirmed the pigment as prodigiosin. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2020.04.011DOI Listing

Quantification of chitooligosaccharides by FACE method: Determination of combinatory effects of mouse chitinases.

MethodsX 2020 18;7:100881. Epub 2020 Apr 18.

Department of Chemistry and Life Science, Kogakuin University, Hachioji, Tokyo, Japan.

Fluorophore-assisted carbohydrate electrophoresis (FACE) enables detection and quantification of degradation products from artificial and natural chitin substrates such as 4-NP-(GlcNAc), (GlcNAc) and colloidal chitin. The FACE method has been improved by our group for analysis of chitooligosaccharides in the presence of several buffer systems commonly used in the biochemical evaluation of chitinolytic activities of enzymes at pH 2.0-8. Read More

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http://dx.doi.org/10.1016/j.mex.2020.100881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183225PMC

Aerobic degradation of dichlorinated dibenzo-p-dioxin and dichlorinated dibenzofuran by bacteria strains obtained from tropical contaminated soil.

Biodegradation 2020 Apr 27;31(1-2):123-137. Epub 2020 Apr 27.

Department of Civil and Environmental Engineering, University of Houston, Houston, TX, 77204-4003, USA.

Bacterial diversity and aerobic catabolic competence of dioxin-degrading bacterial strains isolated from a polluted soil in the tropics were explored. Isolation of bacteria occurred after 12 months of consecutive enrichment, with dioxin congeners serving as the only sources of carbon and energy. Seventeen strains that were isolated were subsequently screened for dioxin metabolic competence. Read More

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http://dx.doi.org/10.1007/s10532-020-09898-8DOI Listing

Corrigendum to 'Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens' [Syst. Appl. Microbiol. 43 (2020) 126055].

Syst Appl Microbiol 2020 May 23;43(3):126082. Epub 2020 Apr 23.

Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute for Food and Nutrition, Hermann-Weigmann-Straße 1, 24103 Kiel, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.syapm.2020.126082DOI Listing

Surveillance of antimicrobial resistance in Serratia marcescens in Mexico.

New Microbiol 2020 Jan 13;43(1):34-37. Epub 2019 Dec 13.

Departamento de Microbiología, Facultad de Medicina y Hospital Universitario "Dr José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.

Antimicrobial resistance is a global public health threat. Therefore, surveillance studies are important tools to help direct antimicrobial use. The aim of this study was to investigate antimicrobial resistance in Serratia marcescens isolates collected in 2016-2017 at eight medical centers from two regions of Mexico. Read More

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January 2020

Genome Sequence of Serratia fonticola Strain S14, Isolated from the Mosquito Aedes triseriatus.

Microbiol Resour Announc 2020 Apr 23;9(17). Epub 2020 Apr 23.

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.

The bacterium strain S14, isolated from the midgut of a female mosquito, has a genome size of 6,176,978 bp. The genome includes genes responsible for acyl-homoserine lactone-mediated quorum sensing, enterobactin, and aerobactin. Read More

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http://dx.doi.org/10.1128/MRA.00099-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180273PMC

Comparative Analysis of Virulence Profiles of Isolated from Diverse Clinical Origins in Mexican Patients.

Surg Infect (Larchmt) 2020 Apr 23. Epub 2020 Apr 23.

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico.

is an enteric bacterium with increasing incidence in clinical settings, attributed mainly to the opportune expression of diverse virulence determinants plus a wide intrinsic and acquired antibiotic resistance. The aim of this study was to compare the virulence factor profiles of 185 isolates from different clinical origins. In vitro proteolytic and hemolytic activities, biofilm formation, and motility were assessed in each strain. Read More

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http://dx.doi.org/10.1089/sur.2020.029DOI Listing

Mobile genetic elements associated with carbapenemase genes in South American Enterobacterales.

Braz J Infect Dis 2020 Apr 21. Epub 2020 Apr 21.

Universidad San Francisco de Quito, Colegio de Ciencias Biológicas y Ambientales, Instituto de Microbiología, Quito, Ecuador.

Introduction: Carbapenem resistance in members of order Enterobacterales is a growing public health problem causing high mortality in developing and industrialized countries. Its emergence and rapid propagation worldwide was due to both intercontinental spread of pandemic strains and horizontal dissemination via mobile genetic elements (MGE) such as plasmids and transposons.

Objective: To describe MGE carrying carbapenem resistance genes in Enterobacterales which have been reported in South America. Read More

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http://dx.doi.org/10.1016/j.bjid.2020.03.002DOI Listing
April 2020
1.096 Impact Factor

New Insights Into Culturable and Unculturable Bacteria Across the Life History of Medicinal Maggots (Meigen) (Diptera: Calliphoridae).

Front Microbiol 2020 8;11:505. Epub 2020 Apr 8.

Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

Because of the nutritional ecology of dung- and carrion-feeding, bacteria are the integral part of life cycle. Nevertheless, the disinfected larvae of the blowfly are applied to treat human chronic wounds in a biosurgery named maggot debridement therapy (MDT). To realize the effects of location/diet on the gut bacteria, to infer the role of bacteria in the blowfly ecology plus in the MDT process, and to disclose bacteria circulating horizontally in and vertically between generations, bacterial communities associated with specimens from various sources were investigated using culture-based and culture-independent methods. Read More

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http://dx.doi.org/10.3389/fmicb.2020.00505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156559PMC
April 2020
3.941 Impact Factor

Mechanism of interaction of an endofungal bacterium Serratia marcescens D1 with its host and non-host fungi.

PLoS One 2020 22;15(4):e0224051. Epub 2020 Apr 22.

Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, India.

Association of bacteria with fungi is a major area of research in infection biology, however, very few strains of bacteria have been reported that can invade and reside within fungal hyphae. Here, we report the characterization of an endofungal bacterium Serratia marcescens D1 from Mucor irregularis SS7 hyphae. Upon re-inoculation, colonization of the endobacterium S. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224051PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176118PMC

Identifying the molecular targets of an anti-pathogenic hydroalcoholic extract of Punica granatum peel against multidrug resistant Serratia marcescens.

Curr Drug Discov Technol 2020 Apr 20. Epub 2020 Apr 20.

Institute of Science, Nirma University, Ahmedabad- 382481. India.

Background: Antibiotic-resistant members of the family Enterobacteriaceae are among the serious threats to human health globally. This study reports anti-pathogenic activity of Punica granatum peel extract (PGPE) against a multi-drug resistant, beta-lactamase producing member of this family i.e. Read More

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http://dx.doi.org/10.2174/1568009620666200421083120DOI Listing