J Virol Methods 2022 Jun 21;307:114571. Epub 2022 Jun 21.
Pathogen Research Laboratory, Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, South Africa; Division of Virology, National Health Laboratory Service, Bloemfontein, South Africa. Electronic address:
Serological assays for detection of IgG, IgM or IgA against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) play an important role in surveillance, antibody persistence, vaccine coverage and infection rate. Serological assays, including both ELISA and rapid lateral flow assays, are available commercially but the cost limits their accessibility for low resource countries. Although serological assays based on mammalian-expressed SARS-CoV-2 spike protein have been previously described these assays need to be validated using samples from local populations within the continent, or country, in which they will be used. Read More