82,654 results match your criteria Scientific reports[Journal]


Isolation of Biopsy-Derived, Human Cervical Keratinocytes Propagated as Monolayer and Organoid Cultures.

Sci Rep 2018 Dec 14;8(1):17869. Epub 2018 Dec 14.

Department of Biology, Lakehead University, Thunder Bay ON, P7B 5E1, Canada.

The successful isolation and propagation of patient-derived keratinocytes from cervical lesions constitute a more appropriate model of cervical disease than traditional cervical cancer-derived cell lines such as SiHa and CaSki. Our aim was to streamline the growth of patient-obtained, cervical keratinocytes into a reproducible process. We performed an observational case series study with 60 women referred to colposcopy for a diagnostic biopsy. Read More

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http://dx.doi.org/10.1038/s41598-018-36150-4DOI Listing
December 2018

Niacin, alkaloids and (poly)phenolic compounds in the most widespread Italian capsule-brewed coffees.

Sci Rep 2018 Dec 14;8(1):17874. Epub 2018 Dec 14.

Human Nutrition Unit, Department of Food and Drugs, University of Parma, Parma, Italy.

Coffee is one of the most popular beverages worldwide and, nowadays, one of the most practical way for its preparation is by prepacked capsules. The aim of this study was comparing the content in caffeine, trigonelline, N-methylpyridinium (NMP), niacin, and chlorogenic acids of 65 different capsule-brewed coffees, commercialised by 5 of the most representative brands in Italy. Coffees were prepared from capsules following manufacturer's instructions and analysed with an optimized UHPLC-MS/MS method able to assess all these phytochemicals in one single run. Read More

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http://dx.doi.org/10.1038/s41598-018-36291-6DOI Listing
December 2018

Deep sequencing and transcriptome analysis to identify genes related to biosynthesis of aristolochic acid in Asarum heterotropoides.

Sci Rep 2018 Dec 14;8(1):17850. Epub 2018 Dec 14.

Traditional Chinese Medicine College, Jilin Agricultural University, Changchun City, Jilin Province, 130118, China.

Asarum spp. are important medicinal plants that have the potential for use in treating various types of fevers. Aristolochic acid is one of the main toxic compounds present in these plants. Read More

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http://www.nature.com/articles/s41598-018-36316-0
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http://dx.doi.org/10.1038/s41598-018-36316-0DOI Listing
December 2018
1 Read

Author Correction: Comparison of 1D and 3D Models for the Estimation of Fractional Flow Reserve.

Sci Rep 2018 Dec 14;8(1):17962. Epub 2018 Dec 14.

National Laboratory for Scientific Computing, LNCC/MCTIC, Av. Getúlio Vargas, 333, Petrópolis-RJ, 25651-075, Brazil.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

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http://dx.doi.org/10.1038/s41598-018-37078-5DOI Listing
December 2018
1 Read

Distinct patterns of transcriptional and epigenetic alterations characterize acute and chronic kidney injury.

Sci Rep 2018 Dec 14;8(1):17870. Epub 2018 Dec 14.

UW Medicine South Lake Union, University of Washington, Seattle, WA, 98109, USA.

Acute kidney injury (AKI) and chronic kidney disease (CKD) are considered early and late phases of a pathologic continuum of interconnected disease states. Although changes in gene expression patterns have recently been elucidated for the transition of AKI to CKD, the epigenetic regulation of key kidney injury related genes remains poorly understood. We used multiplex RT-qPCR, ChIP-qPCR and integrative analysis to compare transcriptional and epigenetic changes at renal disease-associated genes across mouse AKI and CKD models. Read More

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http://dx.doi.org/10.1038/s41598-018-35943-xDOI Listing
December 2018
1 Read

Winged forelimbs of the small theropod dinosaur Caudipteryx could have generated small aerodynamic forces during rapid terrestrial locomotion.

Sci Rep 2018 Dec 14;8(1):17854. Epub 2018 Dec 14.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing, 100044, P. R. China.

Pennaceous feathers capable of forming aerodynamic surfaces are characteristic of Pennaraptora, the group comprising birds and their closest relatives among non-avian dinosaurs. However, members of the basal pennaraptoran lineage Oviraptorosauria were clearly flightless, and the function of pennaceous feathers on the forelimb in oviraptorosaurs is still uncertain. In the basal oviraptorosaur Caudipteryx both the skeleton and the plumage, which includes pennaceous feathers forming wing-like arrangements on the forelimbs, are well known. Read More

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http://dx.doi.org/10.1038/s41598-018-35966-4DOI Listing
December 2018

Cognitively Demanding Object Negotiation While Walking and Texting.

Sci Rep 2018 Dec 14;8(1):17880. Epub 2018 Dec 14.

University of Texas at Austin, Department of Kinesiology & Health Education, Austin, TX, 78712, USA.

Cell phone related pedestrian injuries are increasing, but the underlying causes remain unclear. Here, we studied how cell phone use directly affected obstacle avoidance ability. Thirty healthy adults participated. Read More

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http://dx.doi.org/10.1038/s41598-018-36230-5DOI Listing
December 2018

Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates.

Sci Rep 2018 Dec 14;8(1):17868. Epub 2018 Dec 14.

Ph.D. Program in Clinical Drug Development of Chinese Herbal Medicine, Taipei Medical University, Taipei, Taiwan.

The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells on microplates to improve the coating amount and displayed orientation of capture antibodies. One or eight repeated fragment crystallisable (Fc) binding domains of protein G are stably expressed on the surface of BALB/c 3T3 cells (termed 1pG cells or 8pG cells), which then act as highly antibody-trapping microparticles. Read More

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http://dx.doi.org/10.1038/s41598-018-36192-8DOI Listing
December 2018

Temporal trend and spatial clustering of cholera epidemic in Kumasi-Ghana.

Sci Rep 2018 Dec 14;8(1):17848. Epub 2018 Dec 14.

Faculty of Geo-Information Science and Earth Observation (ITC), University of Twente, Enschede, Netherlands.

Knowledge of the temporal trends and spatial patterns will have significant implications for effective preparedness in future epidemics. Our objective was to investigate the temporal trends and the nature of the spatial interaction of cholera incidences, dwelling on an outbreak in the Kumasi Metropolis, Ghana. We developed generalized nonparametric and segmented regression models to describe the epidemic curve. Read More

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http://www.nature.com/articles/s41598-018-36029-4
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http://dx.doi.org/10.1038/s41598-018-36029-4DOI Listing
December 2018
2 Reads

Impact of ezetimibe on plasma lipoprotein(a) concentrations as monotherapy or in combination with statins: a systematic review and meta-analysis of randomized controlled trials.

Sci Rep 2018 Dec 14;8(1):17887. Epub 2018 Dec 14.

Weill Cornell Medicine Qatar, Doha, Qatar.

The aim of this meta-analysis of randomized placebo-controlled clinical trials was to assess the effect of ezetimibe on plasma lipoprotein(a) concentrations. Only randomized placebo-controlled trials investigating the impact of ezetimibe treatment on cholesterol lowering that include lipoprotein(a) measurement were searched in PubMed-Medline, SCOPUS, Web of Science and Google Scholar databases (from inception to February 26, 2018). A random-effects model and generic inverse variance method were used for quantitative data synthesis. Read More

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http://dx.doi.org/10.1038/s41598-018-36204-7DOI Listing
December 2018
2 Reads

Visualizing the heterogeneous breakdown of a fractal microstructure during compaction by neutron dark-field imaging.

Sci Rep 2018 Dec 14;8(1):17845. Epub 2018 Dec 14.

Laboratory for Neutron Scattering and Imaging, Paul Scherrer Institut, Zurich, Switzerland.

Structural properties of cohesive powders are dominated by their microstructural composition. Powders with a fractal microstructure show particularly interesting properties during compaction where a microstructural transition and a fractal breakdown happen before compaction and force transport. The study of this phenomenon has been challenging due to its long-range effect and the subsequent necessity to characterize these microstructural changes on a macroscopic scale. Read More

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http://www.nature.com/articles/s41598-018-35845-y
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http://dx.doi.org/10.1038/s41598-018-35845-yDOI Listing
December 2018
2 Reads

Individual spindle detection and analysis in high-density recordings across the night and in thalamic stroke.

Sci Rep 2018 Dec 14;8(1):17885. Epub 2018 Dec 14.

Department of Neurology, University Hospital (Inselspital) and University of Bern, Bern, Switzerland.

Sleep spindles are thalamocortical oscillations associated with several behavioural and clinical phenomena. In clinical populations, spindle activity has been shown to be reduced in schizophrenia, as well as after thalamic stroke. Automatic spindle detection algorithms present the only feasible way to systematically examine individual spindle characteristics. Read More

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http://dx.doi.org/10.1038/s41598-018-36327-xDOI Listing
December 2018

Diverse Electronic and Magnetic Properties of Chlorination-Related Graphene Nanoribbons.

Sci Rep 2018 Dec 14;8(1):17859. Epub 2018 Dec 14.

Hi-GEM/Quantum Topology Research Center, National Cheng Kung University, Tainan, 701, Taiwan.

The dramatic changes in electronic and magnetic properties are investigated using the first-principles calculations for halogen(X: Cl, Br, I, At)-adsorbed graphene nanoribbons. The rich and unique features are clearly revealed in the atoms-dominated electronic band structures, spin arrangement/magnetic moment, spatial charge distribution, and orbital- and spin-projected density of states. Halogen adsorptions can create the non-magnetic, ferromagnetic or anti-ferromagnetic metals, being mainly determined by concentrations and edge structures. Read More

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http://dx.doi.org/10.1038/s41598-018-35627-6DOI Listing
December 2018

Plasmodium falciparum infected erythrocytes can bind to host receptors integrins αVβ3 and αVβ6 through DBLδ1_D4 domain of PFL2665c PfEMP1 protein.

Sci Rep 2018 Dec 14;8(1):17871. Epub 2018 Dec 14.

Charles E. Schmidt College of Medicine, Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL, USA.

Major complications and mortality from Plasmodium falciparum malaria are associated with cytoadhesion of parasite-infected erythrocytes (IE). The main parasite ligands for cytoadhesion are members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. Read More

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http://www.nature.com/articles/s41598-018-36071-2
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http://dx.doi.org/10.1038/s41598-018-36071-2DOI Listing
December 2018
1 Read

Fine mapping and discovery of candidate genes for seed size in watermelon by genome survey sequencing.

Sci Rep 2018 Dec 14;8(1):17843. Epub 2018 Dec 14.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, The Laboratory of Melon Crops, Zhengzhou, Henan Province, 450009, China.

Fine mapping and discovery of candidate genes underlying seed size are important for modern watermelon breeding. Here, by using a high-resolution genetic map and whole-genome genetic variation detection aided by genome survey sequencing, we fine mapped and discovered candidate genes for seed size in watermelon. QTL (quantitative trait locus) mapping identified two pleiotropic QTLs for seed size, namely, qSS4 and qSS6, using a high-density genetic map constructed by specific length amplified fragment sequencing. Read More

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http://dx.doi.org/10.1038/s41598-018-36104-wDOI Listing
December 2018

Niche Partitioning in Theropod Dinosaurs: Diet and Habitat Preference in Predators from the Uppermost Cedar Mountain Formation (Utah, U.S.A.).

Sci Rep 2018 Dec 14;8(1):17872. Epub 2018 Dec 14.

Department of Biology, University of Oklahoma, Norman, Oklahoma, 73072, USA.

We explore hypothetical ecologies to explain diversity among predatory dinosaurs in North America's medial Cretaceous, based on occurrence, tooth morphology, and stable isotope analysis. The Mussentuchit local fauna, Utah, USA, is among the best-known terrestrial vertebrate assemblages from the Cretaceous. Study samples include teeth from six microvertebrate sites, ranging in depositional setting from distal floodplain to channel lags. Read More

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http://dx.doi.org/10.1038/s41598-018-35689-6DOI Listing
December 2018

On the role of eye contact in gaze cueing.

Sci Rep 2018 Dec 14;8(1):17842. Epub 2018 Dec 14.

Istituto Italiano di Tecnologia, Social Cognition in Human-Robot Interaction, via Enrico Melen 83, 16152, Genova, Italy.

Most experimental protocols examining joint attention with the gaze cueing paradigm are "observational" and "offline", thereby not involving social interaction. We examined whether within a naturalistic online interaction, real-time eye contact influences the gaze cueing effect (GCE). We embedded gaze cueing in an interactive protocol with the iCub humanoid robot. Read More

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http://www.nature.com/articles/s41598-018-36136-2
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http://dx.doi.org/10.1038/s41598-018-36136-2DOI Listing
December 2018
4 Reads

Gender-specific discrepancy in subjective global assessment for mortality in hemodialysis patients.

Sci Rep 2018 Dec 14;8(1):17846. Epub 2018 Dec 14.

Department of Internal Medicine, College of Medicine, Ewha Womans University, Seoul, Korea.

Although subjective global assessment (SGA) is a widely used representative tool for nutritional investigations even among dialysis patients, no studies have examined gender-specific differences in the ability of SGA to predict mortality in hemodialysis (HD) patients. A total of 2,798 dialysis patients were enrolled from clinical research center for end-stage renal disease (CRC for ESRD) between 2009 and 2015. The cohort was divided into two groups based on nutritional status as evaluated by SGA: 'good nutrition' and 'mild to severe malnutrition'. Read More

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http://www.nature.com/articles/s41598-018-35967-3
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http://dx.doi.org/10.1038/s41598-018-35967-3DOI Listing
December 2018
1 Read

Comparative metabolomics of scab-resistant and susceptible apple cell cultures in response to scab fungus elicitor treatment.

Sci Rep 2018 Dec 14;8(1):17844. Epub 2018 Dec 14.

Plant Molecular Biology Group, Biotechnology Department, Indian Institute of Technology Roorkee, Roorkee, 247667, India.

Apple scab disease caused by the fungus Venturia inaequalis is a devastating disease that seriously affects quality and yield of apples. In order to understand the mechanisms involved in scab resistance, we performed gas chromatography-mass spectrometry based metabolomics analysis of the cell culture of scab resistant cultivar 'Florina' and scab susceptible cultivar 'Vista Bella' both prior -to and -following treatment with V. inaequalis elicitor (VIE). Read More

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http://www.nature.com/articles/s41598-018-36237-y
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http://dx.doi.org/10.1038/s41598-018-36237-yDOI Listing
December 2018
2 Reads

Author Correction: Telomere length and survival in primary cutaneous melanoma patients.

Sci Rep 2018 Dec 14;8(1):17963. Epub 2018 Dec 14.

Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

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http://dx.doi.org/10.1038/s41598-018-36657-wDOI Listing
December 2018

Photonic topological phases in dispersive metamaterials.

Sci Rep 2018 Dec 14;8(1):17881. Epub 2018 Dec 14.

Institute of Applied Mechanics, National Taiwan University, Taipei, 106, Taiwan.

We analyze the photonic topological phases in dispersive metamaterials which satisfy the degenerate condition at a reference frequency. The electromagnetic duality allows for the hybrid modes to be decoupled and described by the spin-orbit Hamiltonians with pseudospin 1, which result in nonzero spin Chern numbers that characterize the topological phases. In particular, the combined Hamiltonian of the hybrid modes complies with a fermionic-like pseudo time-reversal symmetry that ensures the Kramers degeneracy, leading to the topological protection of helical edge states. Read More

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http://dx.doi.org/10.1038/s41598-018-36170-0DOI Listing
December 2018

Publisher Correction: Antimicrobial spray nanocoating of supramolecular Fe(III)-tannic acid metal-organic coordination complex: applications to shoe insoles and fruits.

Sci Rep 2018 Dec 14;8(1):17961. Epub 2018 Dec 14.

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon, 34141, Korea.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

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http://dx.doi.org/10.1038/s41598-018-37133-1DOI Listing
December 2018

Comparison of three variant callers for human whole genome sequencing.

Sci Rep 2018 Dec 14;8(1):17851. Epub 2018 Dec 14.

Computational Biology Unit, Institute of Informatics, University of Bergen, Bergen, Norway.

Testing of patients with genetics-related disorders is in progress of shifting from single gene assays to gene panel sequencing, whole-exome sequencing (WES) and whole-genome sequencing (WGS). Since WGS is unquestionably becoming a new foundation for molecular analyses, we decided to compare three currently used tools for variant calling of human whole genome sequencing data. We tested DeepVariant, a new TensorFlow machine learning-based variant caller, and compared this tool to GATK 4. Read More

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http://dx.doi.org/10.1038/s41598-018-36177-7DOI Listing
December 2018

Miniscule differences between sex chromosomes in the giant genome of a salamander.

Sci Rep 2018 Dec 14;8(1):17882. Epub 2018 Dec 14.

Department of Biology, University of Kentucky, Lexington, KY, USA.

In the Mexican axolotl (Ambystoma mexicanum), sex is determined by a single Mendelian factor, yet its sex chromosomes do not exhibit morphological differentiation typical of many vertebrate taxa that possess a single sex-determining locus. As sex chromosomes are theorized to differentiate rapidly, species with undifferentiated sex chromosomes provide the opportunity to reconstruct early events in sex chromosome evolution. Whole genome sequencing of 48 salamanders, targeted chromosome sequencing and in situ hybridization were used to identify the homomorphic sex chromosome that carries an A. Read More

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http://dx.doi.org/10.1038/s41598-018-36209-2DOI Listing
December 2018

Plasmodium falciparum gametocyte-infected erythrocytes do not adhere to human primary erythroblasts.

Sci Rep 2018 Dec 14;8(1):17886. Epub 2018 Dec 14.

Inserm U1016, Institut Cochin, Paris, France.

Plasmodium falciparum gametocytes, the sexual stages responsible for malaria parasite transmission, develop in the human bone marrow parenchyma in proximity to the erythroblastic islands. Yet, mechanisms underlying gametocytes interactions with these islands are unknown. Here, we have investigated whether gametocyte-infected erythrocytes (GIE) adhere to erythroid precursors, and whether a putative adhesion may be mediated by a mechanism similar to the adhesion of erythrocytes infected with P. Read More

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http://dx.doi.org/10.1038/s41598-018-36148-yDOI Listing
December 2018

Publisher Correction: Obestatin controls skeletal muscle fiber-type determination.

Sci Rep 2018 Dec 14;8(1):17978. Epub 2018 Dec 14.

Área de Endocrinología Celular y Molecular, Instituto de Investigación Sanitaria de Santiago (IDIS), Complejo Hospitalario Universitario de Santiago (CHUS), Servicio Gallego de Salud (SERGAS), Santiago de Compostela, Spain.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. Read More

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http://dx.doi.org/10.1038/s41598-018-36660-1DOI Listing
December 2018

Development of transgenic Caenorhabditis elegans expressing human transthyretin as a model for drug screening.

Sci Rep 2018 Dec 14;8(1):17884. Epub 2018 Dec 14.

Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, Japan.

Familial amyloid polyneuropathy is a hereditary systemic amyloidosis caused by a mutation in the transthyretin (TTR) gene. Amyloid deposits in tissues of patients contain not only full-length TTR but also C-terminal TTR fragments. However, in vivo models to evaluate the pathogenicity of TTR fragments have not yet been developed. Read More

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http://dx.doi.org/10.1038/s41598-018-36357-5DOI Listing
December 2018

Selective overexpression of cytoglobin in stellate cells attenuates thioacetamide-induced liver fibrosis in mice.

Sci Rep 2018 Dec 14;8(1):17860. Epub 2018 Dec 14.

Departments of Hepatology, Graduate School of Medicine, Osaka City University, Osaka, Japan.

Cytoglobin (CYGB), discovered in hepatic stellate cells (HSCs), is known to possess a radical scavenger function, but its pathophysiological roles remain unclear. Here, for the first time, we generated a new transgenic (TG) mouse line in which both Cygb and mCherry reporter gene expression were under the control of the native Cygb gene promoter. We demonstrated that the expression of Cygb-mCherry was related to endogenous Cygb in adult tissues by tracing mCherry fluorescence together with DNA, mRNA, and protein analyses. Read More

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http://www.nature.com/articles/s41598-018-36215-4
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http://dx.doi.org/10.1038/s41598-018-36215-4DOI Listing
December 2018
2 Reads

Repeatability and Reproducibility of Quantification of Superficial Peri-papillary Capillaries by four Different Optical Coherence Tomography Angiography Devices.

Sci Rep 2018 Dec 14;8(1):17866. Epub 2018 Dec 14.

Doheny Image Reading Center, Doheny Eye Institute, Los Angeles, CA, USA.

This study was performed to test the repeatability and reproducibility of measurements of peri-papillary capillaries from four optical coherence tomography angiography (OCTA) devices. 109 healthy eyes were imaged with four OCTA devices (Spectralis, Optovue, Triton and Cirrus). A 3 × 3 mm scan pattern centered on the disc was repeated twice by each device. Read More

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http://dx.doi.org/10.1038/s41598-018-36279-2DOI Listing
December 2018

Probing the dielectric, piezoelectric and magnetic behavior of CoFeO/BNT-BT composite thin film fabricated by sol-gel and spin-coating methods.

Sci Rep 2018 Dec 14;8(1):17883. Epub 2018 Dec 14.

CNR-ISTEC, Institute of Science and Technology for Ceramics, Via Granarolo 64, I-, 48018, Faenza, Italy.

We investigated in this paper a novel bilayer composite obtained by sol-gel and spin coating of the ferroelectric 0.92NaBiTiO-0.08BaTiO (abbreviated as BNT-BT) and ferromagnetic CoFeO phases, for miniature low-frequency magnetic sensors and piezoelectric sensors. Read More

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http://dx.doi.org/10.1038/s41598-018-36232-3DOI Listing
December 2018

Avian Toll-like receptor allelic diversity far exceeds human polymorphism: an insight from domestic chicken breeds.

Sci Rep 2018 Dec 14;8(1):17878. Epub 2018 Dec 14.

Charles University, Faculty of Science, Department of Zoology, Viničná 7, Prague, 12843, Czech Republic.

Immune genes show remarkable levels of adaptive variation shaped by pathogen-mediated selection. Compared to humans, however, population polymorphism in animals has been understudied. To provide an insight into immunogenetic diversity in birds, we sequenced complete protein-coding regions of all Toll-like receptor (TLR) genes with direct orthology between mammals and birds (TLR3, TLR4, TLR5 and TLR7) in 110 domestic chickens from 25 breeds and compared their variability with a corresponding human dataset. Read More

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http://dx.doi.org/10.1038/s41598-018-36226-1DOI Listing
December 2018

Essential oils sensory quality and their bioactivity against the mosquito Aedes albopictus.

Sci Rep 2018 Dec 14;8(1):17857. Epub 2018 Dec 14.

Department of Agriculture, Food and Environment - University of Pisa, Pisa, Italy.

Repellents are a main tool to prevent the outbreak of mosquito-borne diseases that represents a threat for millions of people worldwide. Plant-based products are very promising, low-toxic and eco-friendly alternative to synthetic repellents. Here, we performed an olfactory screening of the essential oils (EOs) of Artemisia verlotiorum Lamotte (Asteraceae), Lavandula dentata L. Read More

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http://dx.doi.org/10.1038/s41598-018-36158-wDOI Listing
December 2018
1 Read

Nucleus-specific X-ray stain for 3D virtual histology.

Sci Rep 2018 Dec 14;8(1):17855. Epub 2018 Dec 14.

Department of Physics and Munich School of BioEngineering, Technical University of Munich, 85748, Garching, Germany.

Histological investigations are indispensable with regards to the identification of structural tissue details but are limited to two-dimensional images, which are often visualized in one and the same plane for comparison reasons. Nondestructive three-dimensional technologies such as X-ray micro- and nanoCT have proven to provide valuable benefits for the understanding of anatomical structures as they allow visualization of structural details in 3D and from arbitrary viewing angles. Nevertheless, low attenuation of soft tissue has hampered their application in the field of 3D virtual histology. Read More

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http://dx.doi.org/10.1038/s41598-018-36067-yDOI Listing
December 2018
1 Read

Aluminum-ceramic composites for thermal management in energy-conversion systems.

Sci Rep 2018 Dec 14;8(1):17852. Epub 2018 Dec 14.

Next-Generation Materials Co., Ltd. (NGM), 1401, Centum Science Park, 79 Centum jungang-ro, Haeundae-gu, Busan, 48058, Republic of Korea.

The most important property of energy-conversion ceramics in high-power lighting devices based on laser diodes (LDs) is thermal durability because high-energy LDs act as excitation and heat sources for ceramics. Herein, aluminum-ceramic composites (ACCs) are introduced for the manipulation of heat generated during high-power lighting. The cerium-doped aluminum garnet (YAG:Ce) phosphor is selected as the energy-conversion ceramic material. Read More

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http://dx.doi.org/10.1038/s41598-018-36270-xDOI Listing
December 2018

Novel Polyheteroarylene Membranes for Separation of Methanol‒Hexane Mixture by Pervaporation.

Sci Rep 2018 Dec 14;8(1):17849. Epub 2018 Dec 14.

Department of Chemical Thermodynamics and Kinetics, Saint Petersburg State University, Saint Petersburg, 198504, Russia.

Polymer membranes with improved transport properties are required for effective separation of organic mixtures (such as methanol‒hexane system) by combination of pervaporation and azeotropic distillation. The present work is devoted to comparative study of two types of membranes based on poly(amidoimide acid) with 2,2'-biquinoline-6,6' units in the backbone; the objects were prepared (i) from the initial polymer and (ii) from the polymer-metal complex (with Cu(I)). Thermo-mechanical and mass spectrometric investigations demonstrated good operational properties of the samples. Read More

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http://dx.doi.org/10.1038/s41598-018-36118-4DOI Listing
December 2018
1 Read

Occurrence and diversity of cyanotoxins in Greek lakes.

Sci Rep 2018 Dec 14;8(1):17877. Epub 2018 Dec 14.

Institute of Nanoscience and Nanotechnology, National Center for Scientific Research "Demokritos", Patr. Grigoriou E' & Neapoleos 27, 15341, Athens, Greece.

Toxic cyanobacteria occur in Greek surface water bodies. However, studies on the occurrence of cyanotoxins (CTs) are often limited to mainly microcystins (MCs), with use of screening methods, such as ELISA, that are not conclusive of the chemical structure of the CT variants and can be subject to false positive results. A multi-lake survey in Greece (14 lakes) was conducted in water and biomass, targeted to a wide range of multi-class CTs including MCs, nodularin-R (NOD), cylindrospermopsin (CYN), anatoxin-a (ANA-a) and saxitoxins (STXs), using multi-class/variant LC-MS/MS analytical workflows, achieving sensitive detection, definitive identification and accurate quantitation. Read More

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http://dx.doi.org/10.1038/s41598-018-35428-xDOI Listing
December 2018

F-doped TiO microporous coating on titanium with enhanced antibacterial and osteogenic activities.

Sci Rep 2018 Dec 14;8(1):17858. Epub 2018 Dec 14.

State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.

To enhance bacterial resistance and osteogenesis of titanium (Ti) -based implants, TiO/calcium-phosphate coatings (TiCP) doped with various amounts of fluorine (F) (designated as TiCP-F1, TiCP-F6, and TiCP-F9) were prepared on Ti by micro-arc oxidation. The F doped TiCP coatings possess a microporous structure (pore size of 3-4 μm in average diameter) which is evenly covered by nano-grains of 30-60 nm in size. Successful F incorporation into TiCP was determined by X-ray photoelectron spectroscopy, and it shows weak influence on the microstructure, phase compositions, surface roughness and wettability of TiCP. Read More

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http://dx.doi.org/10.1038/s41598-018-35875-6DOI Listing
December 2018
1 Read

A novel Lab-on-Fiber Radiation Dosimeter for Ultra-high Dose Monitoring.

Sci Rep 2018 Dec 14;8(1):17841. Epub 2018 Dec 14.

Optoelectronics Group - Department of Engineering, University of Sannio, I-82100, Benevento, Italy.

In this work, we report on the first demonstration of Lab on Fiber (LOF) dosimeter for ionizing radiation monitoring at ultra-high doses. The new dosimeter consists in a metallo-dielectric resonator at sub-wavelength scale supporting localized surface plasmon resonances realized on the optical fiber (OF) tip. The resonating structure involves two gold gratings separated by a templated dielectric layer of poly(methyl methacrylate) (PMMA). Read More

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http://dx.doi.org/10.1038/s41598-018-35581-3DOI Listing
December 2018
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Connectivity of the Superficial Muscles of the Human Perineum: A Diffusion Tensor Imaging-Based Global Tractography Study.

Sci Rep 2018 Dec 14;8(1):17867. Epub 2018 Dec 14.

Department of Bioengineering, Medicine & Radiology, University of California, San Diego, CA, USA.

Despite the importance of pelvic floor muscles, significant controversy still exists about the true structural details of these muscles. We provide an objective analysis of the architecture and orientation of the superficial muscles of the perineum using a novel approach. Magnetic Resonance Diffusion Tensor Images (MR-DTI) were acquired in 10 healthy asymptomatic nulliparous women, and 4 healthy males. Read More

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http://www.nature.com/articles/s41598-018-36099-4
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http://dx.doi.org/10.1038/s41598-018-36099-4DOI Listing
December 2018
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Along-shelf connectivity and circumpolar gene flow in Antarctic silverfish (Pleuragramma antarctica).

Sci Rep 2018 Dec 14;8(1):17856. Epub 2018 Dec 14.

Department of Biology, University of Padua, Via G. Colombo 3, Padua, 35121, Italy.

The Antarctic silverfish (Pleuragramma antarctica) is a critically important forage species with a circumpolar distribution and is unique among other notothenioid species for its wholly pelagic life cycle. Previous studies have provided mixed evidence of population structure over regional and circumpolar scales. The aim of the present study was to test the recent population hypothesis for Antarctic silverfish, which emphasizes the interplay between life history and hydrography in shaping connectivity. Read More

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http://dx.doi.org/10.1038/s41598-018-36030-xDOI Listing
December 2018

Female adipose tissue-specific Bscl2 knockout mice develop only moderate metabolic dysfunction when housed at thermoneutrality and fed a high-fat diet.

Sci Rep 2018 Dec 14;8(1):17863. Epub 2018 Dec 14.

The Rowett Institute, University of Aberdeen, Aberdeen, UK.

Mutations affecting the BSCL2 gene cause the most severe form of congenital generalised lipodystrophy. Affected individuals almost completely lack adipose tissue and suffer from severe diabetes and metabolic complications. Likewise, mice lacking Bscl2 in all tissues have dramatically reduced adipose mass, glucose intolerance and hyperinsulinaemia. Read More

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http://dx.doi.org/10.1038/s41598-018-36078-9DOI Listing
December 2018

Identification of cytochrome P450 monooxygenase genes and their expression in response to high temperature in the alligatorweed flea beetle Agasicles hygrophila (Coleoptera: Chrysomelidae).

Sci Rep 2018 Dec 14;8(1):17847. Epub 2018 Dec 14.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Cytochrome P450 monooxygenases (P450s) are a large class of enzymes that play essential roles in metabolic processes such as hormone synthesis and the catabolism of toxins and other chemicals in insects. In the present study, we identified 82 P450 genes using comprehensive RNA sequencing in the flea beetle Agasicles hygrophila, and all of the sequences were validated by cloning and sequencing. Phylogenetic analysis showed that the P450 genes in A. Read More

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http://dx.doi.org/10.1038/s41598-018-35993-1DOI Listing
December 2018

Nanopore-based detection and characterization of yam viruses.

Sci Rep 2018 Dec 14;8(1):17879. Epub 2018 Dec 14.

CIRAD, BGPI, Montpellier, France.

We here assessed the capability of the MinION sequencing approach to detect and characterize viruses infecting a water yam plant. This sequencing platform consistently revealed the presence of several plant virus species, including Dioscorea bacilliform virus, Yam mild mosaic virus and Yam chlorotic necrosis virus. A potentially novel ampelovirus was also detected by a complimentary Illumina sequencing approach. Read More

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http://dx.doi.org/10.1038/s41598-018-36042-7DOI Listing
December 2018

Serum alkaline phosphatase relates to cardiovascular risk markers in children with high calcium-phosphorus product.

Sci Rep 2018 Dec 14;8(1):17864. Epub 2018 Dec 14.

Pediatric Endocrinology group, (Girona Biomedical Research Institute) IDIBGI, Girona, Spain.

Although alkaline phosphatase (ALP) correlates with cardiovascular risk in adults, there are no studies in children. We evaluated the association between serum ALP levels, calcium-phosphorus product (Ca*P) and cardiovascular risk markers in healthy children. Children aged 7. Read More

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http://www.nature.com/articles/s41598-018-35973-5
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http://dx.doi.org/10.1038/s41598-018-35973-5DOI Listing
December 2018
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Identification of the salusin-β receptor using proteoliposomes embedded with endogenous membrane proteins.

Sci Rep 2018 Dec 14;8(1):17865. Epub 2018 Dec 14.

Protosera Inc., 4-3-22 Nishinakajima, Yodogawa-ku, Osaka, 532-0011, Japan.

Although orphan G protein-coupled receptors (GPCRs) have been used as targets to discover unidentified natural ligands, increasing numbers of non-GPCRs have been found to mediate important biological functions. Bioinformatics of genome and cDNA resources predict putative bioactive peptides, demanding an alternative approach to efficiently unravel cell surface targets. In silico analysis of a full-length cDNA library previously allowed us to identify salusin-β, a parasympathomimetic/pro-atherosclerotic peptide with unique physicochemical properties. Read More

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http://dx.doi.org/10.1038/s41598-018-35740-6DOI Listing
December 2018

DEBIO 1143, an IAP inhibitor, reverses carboplatin resistance in ovarian cancer cells and triggers apoptotic or necroptotic cell death.

Sci Rep 2018 Dec 14;8(1):17862. Epub 2018 Dec 14.

Institut Claudius Regaud - IUCT Oncopole, University Toulouse III, Toulouse, France.

The poor prognosis of ovarian cancer (it is the leading cause of death from gynecological cancers) is mainly due to the acquisition of resistance to carboplatin. Among the possible resistance pathways, resistance to apoptosis and especially the overexpression of inhibitor of apoptosis proteins (IAP) cIAP1 and X-linked IAP (XIAP), have been implicated. DEBIO 1143, a SMAC (second mitochondria-derived activator of caspase) mimetic, belongs to a new class of targeted agents currently being evaluated in clinical trials, which activate apoptotic cell death and block pro-survival signaling in cancer cells. Read More

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http://dx.doi.org/10.1038/s41598-018-35860-zDOI Listing
December 2018

Sleep selectively stabilizes contextual aspects of negative memories.

Sci Rep 2018 Dec 14;8(1):17861. Epub 2018 Dec 14.

Department of Psychiatry, Beth Israel Deaconess Medical Center, Boston, MA, USA.

Sleep and emotion are both powerful modulators of the long-term stability of episodic memories, but precisely how these factors interact remains unresolved. We assessed changes in item recognition, contextual memory, and affective tone for negative and neutral memories across a 12 h interval containing sleep or wakefulness in 71 human volunteers. Our data indicate a sleep-dependent stabilization of negative contextual memories, in a way not seen for neutral memories, item recognition, or across wakefulness. Read More

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http://www.nature.com/articles/s41598-018-35999-9
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http://dx.doi.org/10.1038/s41598-018-35999-9DOI Listing
December 2018
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Photochemically induced dynamic nuclear polarization NMR on photosystem II: donor cofactor observed in entire plant.

Sci Rep 2018 Dec 14;8(1):17853. Epub 2018 Dec 14.

University of Leiden, Leiden Institute of Chemistry, Einsteinweg 55, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.

The solid-state photo-CIDNP (photochemically induced dynamic nuclear polarization) effect allows for increase of signal and sensitivity in magic-angle spinning (MAS) NMR experiments. The effect occurs in photosynthetic reaction centers (RC) proteins upon illumination and induction of cyclic electron transfer. Here we show that the strength of the effect allows for observation of the cofactors forming the spin-correlated radical pair (SCRP) in isolated proteins, in natural photosynthetic membranes as well as in entire plants. Read More

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http://dx.doi.org/10.1038/s41598-018-36074-zDOI Listing
December 2018

The novel EHEC gene asa overlaps the TEGT transporter gene in antisense and is regulated by NaCl and growth phase.

Sci Rep 2018 Dec 14;8(1):17875. Epub 2018 Dec 14.

Lehrstuhl für Mikrobielle Ökologie, Wissenschaftszentrum Weihenstephan, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany.

Only a few overlapping gene pairs are known in the best-analyzed bacterial model organism Escherichia coli. Automatic annotation programs usually annotate only one out of six reading frames at a locus, allowing only small overlaps between protein-coding sequences. However, both RNAseq and RIBOseq show signals corresponding to non-trivially overlapping reading frames in antisense to annotated genes, which may constitute protein-coding genes. Read More

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http://dx.doi.org/10.1038/s41598-018-35756-yDOI Listing
December 2018
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Evaluation of relationships between onychomycosis and vascular diseases using sequential pattern mining.

Sci Rep 2018 Dec 14;8(1):17840. Epub 2018 Dec 14.

Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Onychomycosis (OM) is a common nail disease. Although controversial, vascular diseases are considered independent predictors of OM and vice versa. Sequential pattern mining (SPM) has not been previously used for statistical analysis in dermatology, but it is an efficient method for identifying frequent association rules in multiple sequential data sets. Read More

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http://dx.doi.org/10.1038/s41598-018-35909-zDOI Listing
December 2018
1 Read