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Poult Sci 2022 May 21;101(8):101968. Epub 2022 May 21.

Facultad de Veterinaria, Instituto de Ciencias Biomédicas, Universidad Cardenal Herrera-CEU, CEU Universities, Alfara del Patriarca, Spain.

Salmonellosis remains one of the main foodborne zoonoses in Europe, with poultry products as the main source of human infections. The slaughterhouse has been identified as a potential source for Salmonella contamination of poultry meat. Despite the mandatory programme of the EU, there are companies with persistent Salmonella that are unable to remove the bacteria from their processing environment, compromising the entire production line. Read More

PLoS One 2022 27;17(6):e0269850. Epub 2022 Jun 27.

Reference Laboratory for Microbiology, Executive Department of Reference Laboratories, Research and Laboratories Sector, Saudi Food and Drug Authority (SFDA), Riyadh, Saudi Arabia.

The incidence of hospital- and community-acquired infections has been dramatically increased worldwide. Accordingly, hands hygiene and the use of disinfectants have been increased leading to the expansion in hand sanitizers production to meet public demand. This study was conducted to assess the efficiency of common disinfectants in the market of Riyadh, Saudi Arabia in inhibiting the microbial growth during the time of Coronavirus disease 2019 (COVID-19) pandemic. Read More

Front Microbiol 2022 9;13:899024. Epub 2022 Jun 9.

The Second Clinical Medical College of Nanchang University, Nanchang, China.

is a major zoonotic pathogen, which usually contaminates food resulting in salmonellosis in humans. Exploring the characteristics and origins of is essential in formulating prevention and control measures for infection. We used slide agglutination, antimicrobial susceptibility testing, and whole-genome sequencing to analyze and compare 's phenotype, genotyping diversity, and genetic relatedness from livestock meat and diarrhea patients in Hanzhong, China, from 2018 to 2020. Read More

Front Cell Infect Microbiol 2022 10;12:864087. Epub 2022 Jun 10.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Thrombotic thrombocytopenic purpura (TTP) is one of the two classic thrombotic microangiopathy (TMA) diseases which could be induced by infections. To the best of our knowledge, this is the first report of an acquired immunodeficiency syndrome (AIDS) patient with acquired TTP induced by infection with serovar Typhimurium (hereafter, . Typhimurium) isolate, . Read More

Cureus 2022 May 24;14(5):e25288. Epub 2022 May 24.

Clinical Research, Michigan State University School of Osteopathic Medicine, Detroit, USA.

Giardiasis, a feco-oral route parasitic intestinal infection, and Salmonellosis, a foodborne enteric and extraintestinal bacterial infection, remain major public health issues in countries that lack adequate sanitation, safe water supply, and proper food handling. Here we report a case of and invasive non-typhoidal Salmonella disease co-infection in a patient with a history of recent travel to Mexico. Read More

IJID Reg 2022 Jun 25;3:248-255. Epub 2022 Apr 25.

University of Zambia, School of Veterinary Medicine, Department of Disease Control, Lusaka, Zambia.

Objectives: This study investigated antimicrobial susceptibility and genomic profiling of isolated from bloodstream infections at a tertiary referral hospital in Lusaka, Zambia, 2018-2019.

Method: This was a prospective hospital-based study involving routine blood culture samples submitted to the microbiology laboratory at the University Teaching Hospital. Identification of and determination of antimicrobial susceptibility profiles was achieved through conventional and automated methods. Read More

Poult Sci 2022 May 27;101(8):101977. Epub 2022 May 27.

College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, Shandong, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China. Electronic address:

In order to enrich the knowledge of chicken transcriptomic response to Salmonella enterica serovar Enteritidis infection, 2-day-old chicks were orally inoculated with this bacteria (1.0 × 10 cfu/mL), and then the cecum tissues of 3 days post-inoculation were utilized for RNA sequencing (6 replicates each for treatment group and control group). After analysis, we found a variety of inflammatory genes were triggered at the mRNA level upon infection. Read More

Lancet Infect Dis 2022 Jul;22(7):939

Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address:

BMC Vet Res 2022 Jun 24;18(1):240. Epub 2022 Jun 24.

Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Sciences, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Hefei, 230031, China.

Background: Pullorum disease caused by Salmonella pullorum is one of the most important infectious diseases in the poultry industry, responsible for causing substantial economic losses globally. On farms, the traditional method to detect S. pullorum infection mainly involves the collection of feces and sera to test for antigens and antibodies, respectively, but the regularity of Salmonella pullorum dissemination in internal organs and shedding patterns and antibody production in infected chickens remains unclear. Read More

Lancet Microbe 2022 Jun 21. Epub 2022 Jun 21.

Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA. Electronic address:

Background: The emergence of increasingly antimicrobial-resistant Salmonella enterica serovar Typhi (S Typhi) threatens to undermine effective treatment and control. Understanding where antimicrobial resistance in S Typhi is emerging and spreading is crucial towards formulating effective control strategies.

Methods: In this genomic epidemiology study, we sequenced the genomes of 3489 S Typhi strains isolated from prospective enteric fever surveillance studies in Nepal, Bangladesh, Pakistan, and India (between 2014 and 2019), and combined these with a global collection of 4169 S Typhi genome sequences isolated between 1905 and 2018 to investigate the temporal and geographical patterns of emergence and spread of antimicrobial-resistant S Typhi. Read More

Lancet Microbe 2022 Jun 21. Epub 2022 Jun 21.

Center for Celiac Research and Treatment, MassGeneral Hospital for Children, Boston, MA, USA; Division of Pediatric Gastroenterology and Nutrition, MassGeneral Hospital for Children, Boston, MA, USA; Harvard Medical School, Harvard University, Boston, MA, USA.

Background: The incidence of enteric fever, an invasive bacterial infection caused by typhoidal Salmonellae (Salmonella enterica serovars Typhi and Paratyphi), is largely unknown in regions without blood culture surveillance. The aim of this study was to evaluate whether new diagnostic serological markers for typhoidal Salmonella can reliably estimate population-level incidence.

Methods: We collected longitudinal blood samples from patients with blood culture-confirmed enteric fever enrolled from surveillance studies in Bangladesh, Nepal, Pakistan, and Ghana between 2016 and 2021 and conducted cross-sectional serosurveys in the catchment areas of each surveillance site. Read More

Pharmaceuticals (Basel) 2022 Jun 20;15(6). Epub 2022 Jun 20.

P4T Group, Food and Drug Department, University of Parma, 43124 Parma, Italy.

Antibacterial adjuvants are of great significance, since they allow one to downscale the therapeutic dose of conventional antibiotics and reduce the insurgence of antibacterial resistance. Herein, we report that -acetylserine sulfhydrylase (OASS) inhibitors could be used as colistin adjuvants to treat infections caused by critical pathogens spreading worldwide, , serovar Typhimurium, and . Starting from a hit compound endowed with a nanomolar dissociation constant, we have rationally designed and synthesized a series of derivatives to be tested against Typhimurium OASS isoenzymes, StOASS-A and StOASS-B. Read More

Pathogens 2022 Jun 8;11(6). Epub 2022 Jun 8.

Department of Human Pathology, University of Messina, 98125 Messina, Italy.

Infections caused by bacteria have a major impact on public health-related morbidity and mortality. Despite major advances in the prevention and treatment of bacterial infections, the latter continue to represent a significant economic and social burden worldwide. The WHO compiled a list of six highly virulent multidrug-resistant bacteria named ESKAPE (, , , , , and species) responsible for life-threatening diseases. Read More

Microorganisms 2022 Jun 11;10(6). Epub 2022 Jun 11.

Institute for Food Safety and Hygiene, University of Zurich, CH-8057 Zurich, Switzerland.

subsp. serovar Bovismorbificans has caused multiple outbreaks involving the consumption of produce, hummus, and processed meat products worldwide. To elucidate the intra-serovar genomic structure of . Read More

Int J Mol Sci 2022 Jun 17;23(12). Epub 2022 Jun 17.

Department of Internal Medicine II, Medical University of Innsbruck, 6020 Innsbruck, Austria.

Macrophages are at the center of innate pathogen control and iron recycling. Divalent metal transporter 1 (DMT1) is essential for the uptake of non-transferrin-bound iron (NTBI) into macrophages and for the transfer of transferrin-bound iron from the endosome to the cytoplasm. As the control of cellular iron trafficking is central for the control of infection with siderophilic pathogens such as Typhimurium, a Gram-negative bacterium residing within the phagosome of macrophages, we examined the potential role of DMT1 for infection control. Read More

Int J Mol Sci 2022 Jun 9;23(12). Epub 2022 Jun 9.

Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, Malaysia.

serovar Typhi () is an intracellular pathogen belonging to the Enterobacteriaceae family, where biofilm (aggregation and colonization of cells) formation is one of their advantageous traits. typhi is the causative agent of typhoid fever in the human body and is exceptionally host specific. It is transmitted through the fecal-oral route by consuming contaminated food or water. Read More

Biology (Basel) 2022 May 29;11(6). Epub 2022 May 29.

Department of Anatomy and Physiology, Lithuanian University of Health Sciences, Tilžės g. 18, LT-47181 Kaunas, Lithuania.

  is one of the best adapted bacterial pathogens causing infections in a wide variety of vertebrate species. The aim of this study was to investigate the prevalence of in different reptile species and to evaluate their serological variety and patterns of antimicrobial resistance. In total, 97 samples from 25 wild and domesticated reptile species were investigated in Lithuania. Read More

Diagnostics (Basel) 2022 May 27;12(6). Epub 2022 May 27.

Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.

Recently, CRISPR-Cas system-based assays for bacterial detection have been developed. The aim of this scoping review is to map existing evidence on the utilization of CRISPR-Cas systems in the development of bacterial detection assays. A literature search was conducted using three databases (PubMed, Scopus, and Cochrane Library) and manual searches through the references of identified full texts based on a PROSPERO-registered protocol (CRD42021289140). Read More

Antibiotics (Basel) 2022 Jun 9;11(6). Epub 2022 Jun 9.

Área de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo (UO), 33006 Oviedo, Spain.

serovar Infantis (. Infantis) is a broiler-associated pathogen which ranks in the fourth position as a cause of human salmonellosis in the European Union. Here, we report a comparative genomic analysis of two clinical . Read More

Antibiotics (Basel) 2022 May 28;11(6). Epub 2022 May 28.

Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy.

Nontyphoidal salmonellosis (NTS) is the second most commonly reported gastrointestinal infection in humans and an important cause of food-borne outbreaks in Europe. The use of antimicrobial agents for animals, plants, and food production contributes to the development of antibiotic-resistant strains that are transmissible to humans through food. The aim of this study was to investigate the presence and the potential dissemination of multidrug-resistant (MDR) strains isolated in the Marche Region (Central Italy) via the food chain. Read More

Antioxidants (Basel) 2022 May 24;11(6). Epub 2022 May 24.

Millenium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.

An important virulence trait of serovar Typhimurium ( Typhimurium) is the ability to avoid the host immune response, generating systemic and persistent infections. Host cells play a crucial role in bacterial clearance by expressing the enzyme heme oxygenase 1 (Hmox1), which catalyzes the degradation of heme groups into Fe, biliverdin, and carbon monoxide (CO). The role of Hmox1 activity during Typhimurium infection is not clear and previous studies have shown contradictory results. Read More

Clin Microbiol Infect 2022 Jun 20. Epub 2022 Jun 20.

Department of Clinical Sciences, Institute of Tropical Medicine - Antwerp Belgium.

Objectives: Azithromycin is an alternative to treat invasive non-typhoidal Salmonella (iNTS) infections. We determined its epidemiological cut-off (ECOFF) and compared azithromycin susceptibility testing methods for iNTS.

Methods: We used EUCAST ECOFFinder to determine the minimum inhibitory concentrations (MIC; obtained by broth microdilution) ECOFF and corresponding disk zone diameters of 515 iNTS from blood cultures in DR Congo, Burkina Faso, Rwanda, and Cambodia. Read More

Vet Sci 2022 May 31;9(6). Epub 2022 May 31.

Facultad de Veterinaria, Instituto de Ciencias Biomédicas, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Alfara del Patriarca, Spain.

is mostly noted as a food-borne pathogen, but contact with chelonians has also been reported as a source of infection. Moreover, high levels of antimicrobial resistance (AMR) have been reported in isolated from wild and captive reptiles. The aim of this study was to assess the occurrence of AMR carriage by chelonians admitted to two zoological institutions in Spain, characterizing the isolates to assess the AMR epidemiology in wildlife. Read More

Bull Exp Biol Med 2022 Jun 23. Epub 2022 Jun 23.

Federal Research Center of Nutrition and Biotechnology, Moscow, Russia.

The review is devoted to the issues of improving the food biosafety system by developing and implementing modern methods of microbiological and molecular genetic analysis and new safety standards for the main groups of food products. The results of fundamental and applied microbiological research in the field of food safety conducted by the Federal Research Center for Nutrition and Biotechnology are presented. We demonstrated the necessity of a detailed study of the ecology and features of survival of new types of pathogenic microorganisms and evaluation of the role of technological factors in the formation of altered properties of these pathogens in food production environment. Read More

J Clin Microbiol 2022 Jun 23:e0002122. Epub 2022 Jun 23.

bioMérieux, La Balme les Grottes, France.

Fosfomycin is a phosphonic acid derivative active against a wide spectrum of Gram-positive and Gram-negative pathogens. It is used for the treatment of uncomplicated urinary tract infections (uUTI) or severe infections by oral or intravenous (i.v. Read More

Biosensors (Basel) 2022 May 26;12(6). Epub 2022 May 26.

DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad 500032, India.

Salmonellosis is a major cause of foodborne infections, caused by , posing a major health risk. It possesses the ability to infiltrate the food supply chain at any point throughout the manufacturing, distribution, processing or quality control process. infection has increased severely and requires effective and efficient methods for early monitoring and detection. Read More

Isr Med Assoc J 2022 Jun;24(6):360-363

Israel Center for Disease Control, Israel Ministry of Health, Ramat Gan, Israel.

Background: Salmonella, Shigella, and Campylobacter are highly prevalent among children. Reports on risk factors of patients infected with all three pathogens, not simultaneously, are scarce.

Objectives: To identify risk factors for multiple infection with Salmonella, Shigella, and Campylobacter in the same child. Read More

Gut Microbes 2022 Jan-Dec;14(1):2089007

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing, China.

Some serovars of are not or rare found to cause salmonellosis in human. In our clinic-based surveillance, three rare 4,5,12:a:- strains were recovered from three patients with diarrhea. To explore their genetic and epidemiological characteristics and pathogenesis, we conducted whole-genome sequencing, invasion assays in mammalian cells, and virulence assays in an animal model. Read More

Front Microbiol 2022 6;13:906238. Epub 2022 Jun 6.

IRSD - Institut de Recherche en Santé Digestive, Université́ de Toulouse, INSERM, INRAE, ENVT, UPS, Toulouse, France.

serovars are invasive gram-negative bacteria, causing a wide range of diseases from gastroenteritis to typhoid fever, representing a public health threat around the world. gains access to the intestinal lumen after oral ingestion of contaminated food or water. The crucial initial step to establish infection is the interaction with the intestinal epithelium. Read More

Vet Res 2022 Jun 22;53(1):46. Epub 2022 Jun 22.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

A universal vaccine protecting against multiple serotypes of Streptococcus suis is urgently needed to improve animal welfare and reduce the consumption of antibiotics. In this study, a dual antigen expression cassette consisting of SS2-SaoA and SS9-Eno was delivered by a recombinant Salmonella Choleraesuis vector to form the vaccine candidate rSC0016(pS-SE). SaoA and Eno were simultaneously synthesized in rSC0016(pS-SE) without affecting the colonization of the recombinant vector in the lymphatic system. Read More