4,665 results match your criteria Rhodococcus equi


Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective -Estradiol-Degrading Bacterium.

Int J Genomics 2018 28;2018:3505428. Epub 2018 Oct 28.

School of Environment, Northeast Normal University, No. 2555 Jingyue Avenue, Changchun City, Jilin Province, China.

We screened bacteria that use E2 as its sole source of carbon and energy for growth and identified them as Rhodococcus, and we named them DSSKP-R-001. For a better understanding of the metabolic potential of the strain, whole genome sequencing of Rhodococcus DSSKP-R-001 and annotation of the functional genes were performed. The genomic sketches included a predicted protein-coding gene of approximately 5. Read More

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October 2018

Genomic Analysis of sp. Br-6, a Bromate Reducing Bacterium Isolated From Soil in Chiba, Japan.

J Genomics 2018 15;6:122-126. Epub 2018 Nov 15.

Graduate School of Horticulture, Chiba University, Matsudo City, Chiba, Japan.

Bromate is a byproduct of the ozone disinfection of drinking water. It is a genotoxic carcinogen and causes renal cell tumors in rats. Physicochemical removal of bromate is very difficult, making microbial reduction of bromate to bromide a promising approach to eliminate bromate from water. Read More

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November 2018

Optimizing the metribuzin degrading potential of a novel bacterial consortium based on Taguchi design of experiment.

J Hazard Mater 2018 Nov 16;366:1-9. Epub 2018 Nov 16.

Department Environmental Biotechnology, Helmholtz Centre for Environmental Research, Permoserstr. 15, Leipzig, Germany.

Metribuzin (MB) is used for control of weeds in crops like potato, maize and sugarcane. Its extensive and unjudicial use has resulted in various environmental issues; hence it is very critical to remediate this herbicide at the respective point source. Plant associated, MB degrading bacterial strains, Rhodococcus rhodochrous sp. Read More

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November 2018

Poplar phyllosphere harbors disparate isoprene-degrading bacteria.

Proc Natl Acad Sci U S A 2018 Nov 29. Epub 2018 Nov 29.

School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.

The climate-active gas isoprene (2-methyl-1,3-butadiene) is released to the atmosphere in huge quantities, almost equaling that of methane, yet we know little about the biological cycling of isoprene in the environment. Although bacteria capable of growth on isoprene as the sole source of carbon and energy have previously been isolated from soils and sediments, no microbiological studies have targeted the major source of isoprene and examined the phyllosphere of isoprene-emitting trees for the presence of degraders of this abundant carbon source. Here, we identified isoprene-degrading bacteria in poplar tree-derived microcosms by DNA stable isotope probing. Read More

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November 2018

The effect of bioaugmentation of petroleum-contaminated soil with Rhodococcus erythropolis strains on removal of petroleum from soil.

Ecotoxicol Environ Saf 2018 Nov 26;169:615-622. Epub 2018 Nov 26.

Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland. Electronic address:

The aim of the study was to assess the impact of inoculation of petroleum-contaminated soil with the hydrocarbon-degrading bacterial strains Rhodococcus erythropolis CD 130 and CD 167 or their consortium on the removal of hydrocarbons from the soil. Additionally, changes in the activity and structure of soil autochthonous bacterial communities were studied. At the end of the experiment, the fastest hydrocarbon removal was seen in the soil treated with the CD 167 strain (38. Read More

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November 2018
1 Read

Characterization of a Carbonyl Reductase from WZ010 and Its Variant Y54F for Asymmetric Synthesis of ()--Boc-3-Hydroxypiperidine.

Molecules 2018 Nov 28;23(12). Epub 2018 Nov 28.

Grain and Oil Products Quality Inspection Center of Zhejiang Province, Hangzhou 310012, China.

The recombinant carbonyl reductase from WZ010 (ReCR) demonstrated strict ()-stereoselectivity and catalyzed the irreversible reduction of -Boc-3-piperidone (NBPO) to ()--Boc-3-hydroxypiperidine [()-NBHP], a key chiral intermediate in the synthesis of ibrutinib. The NAD(H)-specific enzyme was active within broad ranges of pH and temperature and had remarkable activity in the presence of higher concentration of organic solvents. The amino acid residue at position 54 was critical for the activity and the substitution of Tyr54 to Phe significantly enhanced the catalytic efficiency of ReCR. Read More

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November 2018

Microbial life beyond the grave: 16S rRNA gene-based metagenomic analysis of bacteria diversity and their functional profiles in cemetery environments.

Sci Total Environ 2018 Nov 22;655:831-841. Epub 2018 Nov 22.

Engineering Geology and Hydrogeology, Department of Geology, University of Pretoria, Pretoria 0028, South Africa. Electronic address:

Recent studies have identified cemeteries as potential environmental reservoirs of multi-drug resistant pathogenic bacteria that could contaminate groundwater sources posing public health threats. However, these findings were based on the identification of culturable bacteria and at times not below burial grounds. Investigation on the bacterial diversity and functional profiles of bacterial communities above and below burial grounds in human cemeteries are few. Read More

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November 2018
4 Reads

Second line molecular diagnosis for bovine tuberculosis to improve diagnostic schemes.

PLoS One 2018 26;13(11):e0207614. Epub 2018 Nov 26.

University Paris-Est, French Agency for Food, Environmental and Occupational Health and Safety (Anses), Laboratory for Animal Health, Maisons-Alfort, France.

Surveillance of bovine tuberculosis (bTB) is partly based on the sanitary inspection of carcasses at the abattoir to detect bTB-like lesions which, in compliance with EU recommendations, are analysed by bacteriology and histopathology to disclose Mycobacterium bovis (or M. caprae) infection. Moreover, since 2012, a PCR method with similar sensitivity and specificity values of histopathology and bacteriology respectively is additionally employed in France, partially compensating for the weaknesses of classical diagnostic methods. Read More

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November 2018

Cloning, characterization and paper pulp applications of a newly isolated DyP type peroxidase from Rhodococcus sp. T1.

Mol Biol Rep 2018 Nov 24. Epub 2018 Nov 24.

Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.

A newly identified ligninolytic Rhodococcus strain (Rhodococcus sp. T1) was isolated from forestry wastes (Trabzon/Turkey). The DyP type peroxidase of Rhodococcus sp. Read More

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November 2018
2 Reads

A novel integrated biodegradation-microfiltration system for sustainable wastewater treatment and energy recovery.

J Hazard Mater 2018 Nov 11;365:707-715. Epub 2018 Nov 11.

Center for the Environment, Indian Institute Technology Guwahati, Guwahati, Assam 781039, India; Department of Chemical Engineering, Indian Institute Technology Guwahati, Guwahati, Assam 781039, India.

This work assessed the treatment of wastewater generated from three different industries viz., paper and pulp, biomass gasification and dairy by biodegradation followed by membrane filtration. Batch biodegradation was first carried out using wastewater as the potential substrate for oleaginous Rhodococcus opacus with lipid accumulation intracellular; subsequently, a microfiltration system was applied to recover the bacterial biomass grown as well as for residual chemical oxygen demand (COD) removal from the effluent. Read More

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November 2018

Rhodococcus daqingensis sp. nov., isolated from petroleum-contaminated soil.

Antonie Van Leeuwenhoek 2018 Nov 22. Epub 2018 Nov 22.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

A novel Gram-stain positive, aerobic, non-motile bacterial strain, designated Z1, was isolated from a sample of petroleum-contaminated soil collected in Daqing, Heilongjiang province, China and characterised with a series of taxonomic approaches. The morphological and chemotaxonomic properties of the isolate were typical of those of members of the genus Rhodococcus. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Z1 belongs to the genus Rhodococcus and clustered with Rhodococcus maanshanensis DSM 44675 (99. Read More

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November 2018
3 Reads

Chronic cavitary pneumonia by Rhodococcus equi in a highly prevalent tuberculosis country: a diagnosis challenge.

Rev Inst Med Trop Sao Paulo 2018 Nov 14;60:e74. Epub 2018 Nov 14.

Universidade Federal do Rio Grande do Norte, Departamento de Infectologia, Natal, Rio Grande do Norte, Brazil.

Rhodococcus equi is a facultative aerobic, intracellular, non-motile, non-spore-forming, Gram-positive, weakly acid-fast coccobacillus belonging to the group of nocardioform actinomycetes. R. equi infections are rare opportunistic illnesses in patients with Acquired Immunodeficiency Syndrome (AIDS), associated with a high mortality rate. Read More

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November 2018
3 Reads

iTRAQ-based comparative proteomic analysis of differentially expressed proteins in Rhodococcus sp. BAP-1 induced by fluoranthene.

Ecotoxicol Environ Saf 2018 Nov 17;169:282-291. Epub 2018 Nov 17.

College of Environment and Resource, Guangxi Normal University, 541004 Guilin, Guangxi, China. Electronic address:

To reveal the molecular mechanism at the level of regulation of proteins in Rhodococcus sp. BAP-1 induced by fluoranthene comparative proteomic analysis was performed on proteins extracted from fluoranthene-exposed cells on 1 d, 3 d, 6 d and 8 d compared with control cells using isobaric tags for relative and absolute quantization (iTRAQ) labeling and LC-MS/MS analysis to access differentially expressed proteins. As a result, we detected a total of 897 significantly differentially expressed proteins, including 30 shared proteins in four comparison clusters. Read More

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November 2018
1 Read

Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms.

Environ Sci Pollut Res Int 2018 Nov 20. Epub 2018 Nov 20.

Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.

This study investigates the biodegradation of crude oil by a mixed culture of bacteria isolated from the Qinghai-Tibet plateau using gas chromatography-mass spectrometer (GC-MS) and the gravimetric method. The results showed that a mixed culture has a stronger ability to degrade hydrocarbon than pure cultures. Once both Nocardia soli Y48 and Rhodococcus erythropolis YF28-1 (8) were present in a culture, the culture demonstrated the highest crude oil removal efficiency of almost 100% after 10 days of incubation at 20 °C. Read More

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November 2018
2 Reads

Fabrication of MgO nanostructures and its efficient photocatalytic, antibacterial and anticancer performance.

J Photochem Photobiol B 2018 Nov 12;190:8-20. Epub 2018 Nov 12.

Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappali 620 024, Tamilnadu, India.

Magnesium oxide (MgO) nanostructures were prepared using microwave-assisted (M 1) and hydrothermal (M 2) methods and characterized by XRD, SEM and FT-IR. It exhibits cubic structure with an average crystallite size of 20 nm (M 1) and 14 nm (M 2) and the lattice strain (WH plot) is 0.0017 (M 1), 0. Read More

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November 2018
3 Reads

Isolation and characterization of aerobic, culturable, arsenic-tolerant bacteria from lead-zinc mine tailing in southern China.

World J Microbiol Biotechnol 2018 Nov 16;34(12):177. Epub 2018 Nov 16.

College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People's Republic of China.

Bioremediation of arsenic (As) pollution is an important environmental issue. The present investigation was carried out to isolate As-resistant novel bacteria and characterize their As transformation and tolerance ability. A total of 170 As-resistant bacteria were isolated from As-contaminated soils at the Kangjiawan lead-zinc tailing mine, located in Hunan Province, southern China. Read More

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November 2018
3 Reads

The Enhancement of Pyridine Degradation by Rhodococcus KDPy1 in Coking Wastewater.

FEMS Microbiol Lett 2018 Nov 15. Epub 2018 Nov 15.

College of Life Science, University of Chinese Academy of Sciences, A19 Yuquan Road, Beijing 100049, China.

Pyridine is a typical nitrogen heterocyclic and recalcitrant organic compound in coking wastewater. The pyridine-degrading bacterial strain KDPy1 was isolated from aerobic sludge in a coking wastewater treatment plant. The homology analysis based on 16S rDNA sequences suggested that KDPy1 belonged to Rhodococcus sp. Read More

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November 2018

Construction and Evaluation of a Korean Native Microbial Consortium for the Bioremediation of Diesel Fuel-Contaminated Soil in Korea.

Front Microbiol 2018 30;9:2594. Epub 2018 Oct 30.

Department of Life Science, Chung-Ang University, Seoul, South Korea.

A native microbial consortium for the bioremediation of soil contaminated with diesel fuel in Korea was constructed and its biodegradation ability was assessed. Microbial strains isolated from Korean terrestrial environments, with the potential to biodegrade aliphatic hydrocarbons, PAHs, and resins, were investigated and among them, eventually seven microbial strains, DR1, sp. KSS-2, sp. Read More

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October 2018
2 Reads

Metallochaperone Function of the Self-Subunit Swapping Chaperone Involved in the Maturation of Subunit-Fused Cobalt Type Nitrile Hydratase.

Biotechnol Bioeng 2018 Nov 12. Epub 2018 Nov 12.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

The transition metal (iron or cobalt) is a mandatory part that constitute the catalytic center of Nitrile hydratase (NHase). The incorporation of cobalt ion into cobalt containing NHase (Co-NHase) was reported to depend on self-subunit swapping, and the activator of the Co-NHase acts as a self-subunit swapping chaperone for subunit exchange. Here we discovered that the activator acted as a metallochaperone transferred cobalt ion into subunit-fused Co-NHase. Read More

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November 2018

Antimicrobial agent isolated from Coptidis rhizome extract incubated with Rhodococcus sp. strain BD7100.

J Antibiot (Tokyo) 2018 Nov 9. Epub 2018 Nov 9.

Department of Organic Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan.

Coptidis rhizome (CR) is a widely used herbal medicine that contains protoberberine-type alkaloids. CR extract exhibits various pharmacologic activities. A previous study reported the isolation of Rhodococcus sp. Read More

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November 2018
2 Reads

4-Chlorophenol Oxidation Depends on the Activation of an AraC-Type Transcriptional Regulator, CphR, in sp. Strain YH-5B.

Front Microbiol 2018 23;9:2481. Epub 2018 Oct 23.

Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, Singapore, Singapore.

4-Chlorophenol (4-CP) oxidation plays an essential role in the detoxification of 4-CP. However, oxidative regulation of 4-CP at the genetic and biochemical levels has not yet been studied. To explore the regulation mechanism of 4-CP oxidation, a novel gene cluster, , involved in biodegradation of 4-CP was identified and cloned from sp. Read More

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October 2018
1 Read
3.940 Impact Factor

Social microbial inocula confer functional stability in a methyl tert-butyl ether extractive membrane biofilm bioreactor.

Environ Pollut 2019 Jan 25;244:855-860. Epub 2018 Oct 25.

Environmental Microbiology Group, Institute of Water Research, University of Granada, Granada, Spain; Department of Microbiology, University of Granada, Granada, Spain.

Methyl tert-butyl ether (MTBE) degradation technologies based on two-phase partitioning systems such as extractive membrane biofilm reactors (EMBFR) permit separation of biological and contaminant compartments, thus allowing optimization of the biological section. In this study, we set-up an EMBFR with three MTBE-degrading and cooperating strains (termed social biofilm: Agrobacterium sp. MS2, Paenibacillus etheri SH7 and Rhodococcus ruber EE6). Read More

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January 2019
1 Read

A new functional biofilm biocatalyst for the simultaneous removal of dibenzothiophene and quinoline using and curli amyloid overproducer mutants derived from sp. strain MM1IDA2H-1.

Biotechnol Rep (Amst) 2018 Dec 12;20:e00286. Epub 2018 Oct 12.

Escuela de Nutrición, Facultad de Farmacia, Universidad de Valparaíso, Casilla, 5001, Valparaíso, Chile.

Biocatalyst systems based on biofilms were developed to remove nitrogen and sulfur-containing heterocyclic hydrocarbons using sp. strain MM1IDA2H-1 and . The overproducers mutants CM and CM were derived from C sp. Read More

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December 2018
4 Reads

Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.

Front Microbiol 2018 16;9:2441. Epub 2018 Oct 16.

Department of Biotransformation, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.

BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product was identified as the trehalose lipid with a molecular mass of 848 g mol. The purified biosurfactant reduced the surface tension of water from 72 to 34 mN m. Read More

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October 2018
10 Reads

Biocompatible metal-assisted C-C cross-coupling combined with biocatalytic chiral reductions in a concurrent tandem cascade.

Chem Commun (Camb) 2018 Nov;54(92):12978-12981

Institute of Applied Synthetic Chemistry, 163-OC, TU Wien, 1060 Vienna, Austria.

In this study, we present a concurrent chemo/biocatalytic one pot reaction cascade by combining a metal (Pd/Cu) assisted Liebeskind-Srogl (L-S) coupling with an enantioselective enzymatic reduction for the production of chiral amines and alcohols. The latter transformation was realized by applying enantiocomplementary alcohol dehydrogenases from Lactobacillus kefir (R-selective) and Rhodococcus ruber (S-selective). Compatibility issues were solved by investigating first the L-S-coupling protocol in water at room temperature. Read More

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November 2018
2 Reads

Microbial Degradation of Epoxy.

Materials (Basel) 2018 Oct 29;11(11). Epub 2018 Oct 29.

Mass Spectrometry Laboratory, School of Chemistry, Faculty of Exact Sciences, Tel-Aviv University, Ramat Aviv, Tel Aviv 6997801, Israel.

Epoxy resins have a wide range of applications, including in corrosion protection of metals, electronics, structural adhesives, and composites. The consumption of epoxy resins is predicted to keep growing in the coming years. Unfortunately, thermoset resins cannot be recycled, and are typically not biodegradable. Read More

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October 2018
2 Reads

Two dcm Gene Clusters Essential for the Degradation of Diclofop-methyl in a Microbial Consortium of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9.

J Agric Food Chem 2018 Nov 6;66(46):12217-12226. Epub 2018 Nov 6.

Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute , Nanyang Technological University , 1 Cleantech Loop , Singapore 637141 , Singapore.

The metabolism of widely used aryloxyphenoxypropionate herbicides has been extensively studied in microbes. However, the information on the degradation of diclofop-methyl (DCM) is limited, with no genetic and biochemical investigation reported. The consortium L1 of Rhodococcus sp. Read More

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November 2018
3 Reads
2.912 Impact Factor

Emergence of resistance to macrolides and rifampicin in clinical isolates of from foals in central Kentucky, USA: 1995 to 2017.

Antimicrob Agents Chemother 2018 Oct 29. Epub 2018 Oct 29.

Veterinary Diagnostic Laboratory, University of Kentucky, Lexington, Kentucky, USA.

To determine the prevalence of strains resistant to macrolides and rifampicin over time in clinical samples from foals submitted to diagnostic laboratories in central Kentucky. Retrospective observational study of all clinical samples from foals that were submitted to veterinary diagnostics laboratories in Kentucky between January 1995 and December 2017. Samples were included if was cultured and tested for susceptibility to erythromycin or rifampicin. Read More

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October 2018
3 Reads
4.480 Impact Factor

Increasing lipid production using an NADP-dependent malic enzyme from Rhodococcus jostii.

Microbiology 2018 Oct 29. Epub 2018 Oct 29.

Facultad de Ciencias Naturales, Instituto de Biociencias de la Patagonia, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de la Patagonia San Juan Bosco, Ruta Provincial no. 1, Km 4-Ciudad Universitaria, 9000 Comodoro Rivadavia, Chubut, Argentina.

The occurrence of NADP-dependent malic enzymes (NADP-MEs) in several Rhodococcus strains was analysed. The NADP-ME number in Rhodococcus genomes seemed to be a strain-dependent property. Total NADP-ME activity increased by 1. Read More

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October 2018
11 Reads

Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants.

Front Microbiol 2018 12;9:2410. Epub 2018 Oct 12.

Microbial Biotechnology, Department of Biology and Biotechnology, Ruhr-University Bochum, Bochum, Germany.

Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from 1CP (WT) as well as its engineered variants C25A, C25S and C25G. OYERo2a in its redox resting state (oxidized form) is irreversibly inactivated by -methylmaleimide. Read More

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October 2018
6 Reads
3.940 Impact Factor

Structural and kinetic characterization of (S)-1-amino-2-propanol kinase from the aminoacetone utilization microcompartment of .

J Biol Chem 2018 Oct 25. Epub 2018 Oct 25.

Molecular and Cellular Biology, University of Guelph, Canada.

Bacterial microcompartments encapsulate enzymatic pathways that generate small, volatile, aldehyde intermediates. The Rhodococcus and Mycobacterium microcompartment (RMM) operon from Mycobacterium smegmatis encodes four enzymes, including S-1-amino-2-propanol dehydrogenase and a likely propionaldehyde dehydrogenase. We show here that a third enzyme (and its non-microcompartment-associated paralog) is a moderately specific (S)-1-amino-2-propanol kinase. Read More

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October 2018

Characterization of MK₈(H₂) from sp. B7740 and Its Potential Antiglycation Capacity Measurements.

Mar Drugs 2018 Oct 18;16(10). Epub 2018 Oct 18.

Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK₂), which is mainly produced through the fermentation of microorganisms. MK₈(H₂) was identified to be the main menaquinone from sp. B7740, a bacterium isolated from the arctic ocean. Read More

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October 2018
1 Read

Studying NAD(P)H cofactor-binding to alcohol dehydrogenases through global analysis of circular dichroism spectra.

Phys Chem Chem Phys 2018 Oct 17. Epub 2018 Oct 17.

Institute of Pharmaceutical Sciences, University of Freiburg, Albertstraße 25, 79104 Freiburg, Germany.

The initial step in reactions catalyzed by NAD(P)H-dependent alcohol dehydrogenases (ADHs) is the binding of the cofactor to the active site. To study this process, we measured NAD(P)H concentration-dependent circular dichroism (CD) in the presence of purified enzymes (ADH from horse liver, HLADH; ADH-A from Rhodococcus ruber; YGL157w from Saccharomyces cerevisiae) or enzyme-containing whole cell extract (ADH from Lactobacillus brevis, LbADH). We determined the proportions of binding and non-binding NAD(P)H and the associated dissociation constants (Kd) from matrix least-squares global fitting of law of mass action-derived model. Read More

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October 2018
1 Read

Biodegradation of di-(2-ethylhexyl) phthalate by a halotolerant consortium LF.

PLoS One 2018 15;13(10):e0204324. Epub 2018 Oct 15.

Institute of Sustainable Development in Agriculture and Rural Area, Henan University, Kaifeng, Henan, China.

A halotolerant bacterial consortium capable of degrading di-(2-ethylhexyl) phthalate (DEHP) was enriched from activated sludge. Community analysis revealed that LF contained seven families and seven genera of bacteria. The predominant species was Gordonia sp. Read More

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October 2018
5 Reads

The gut bacteria across life stages in the synanthropic fly Chrysomya megacephala.

BMC Microbiol 2018 Oct 11;18(1):131. Epub 2018 Oct 11.

Hubei International Scientific and Technological Cooperation Base of Waste Conversion by Insects, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Gut bacteria are closely associated with host. Chrysomya megacephala, as a vector and resource insect, can transmit various pathogenic bacteria and consume manure to produce biofertilizer and larva biomass. However, the gut bacteria composition and abundance of C. Read More

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October 2018
3 Reads

Characterization and use of a bacterial lignin peroxidase with an improved manganese-oxidative activity.

Appl Microbiol Biotechnol 2018 Dec 9;102(24):10579-10588. Epub 2018 Oct 9.

Department of Biotechnology and Life Sciences, University of Insubria, via Dunant 3, 21100, Varese, Italy.

Peroxidases are well-known biocatalysts produced by all organisms, especially microorganisms, and used in a number of biotechnological applications. The enzyme DypB from the lignin-degrading bacterium Rhodococcus jostii was recently shown to degrade solvent-obtained fractions of a Kraft lignin. In order to promote the practical use, the N246A variant of DypB, named Rh_DypB, was overexpressed in E. Read More

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December 2018
1 Read
3.340 Impact Factor

Isolation and Transcriptome Analysis of Phenol-Degrading Bacterium From Carbon-Sand Filters in a Full-Scale Drinking Water Treatment Plant.

Front Microbiol 2018 21;9:2162. Epub 2018 Sep 21.

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China.

Phenol is a typical organic contaminant in the environment. To date, the biodegradation of phenol by microorganisms remains the preferred method for its removal and remediation, but data on phenol removal by drinking water biofilters are lacking. In this study, we used high-throughput sequencing to investigate the microbial community structure in a carbon-sand biofilter. Read More

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September 2018
3 Reads

Identification of a VapA virulence factor functional homolog in Rhodococcus equi isolates housing the pVAPB plasmid.

PLoS One 2018 4;13(10):e0204475. Epub 2018 Oct 4.

Department of Infectious Disease, University of Georgia, Athens, Georgia, United States of America.

Rhodococcus equi is a facultative intracellular bacterium of macrophages and is an important pathogen of animals and immunocompromised people wherein disease results in abcessation of the lungs and other sites. Prior work has shown that the presence of the major virulence determinant, VapA, encoded on the pVAPA-type plasmid, disrupts normal phagosome development and is essential for bacterial replication within macrophages. pVAPA- type plasmids are typical of R. Read More

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October 2018

Response of microbial community structure and metabolic profile to shifts of inlet VOCs in a gas-phase biofilter.

AMB Express 2018 Oct 3;8(1):160. Epub 2018 Oct 3.

Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing, China.

The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. Read More

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October 2018
1 Read

Biodegradation of high-molecular weight PAHs by Rhodococcus wratislaviensis strain 9: Overexpression of amidohydrolase induced by pyrene and BaP.

Sci Total Environ 2019 Feb 18;651(Pt 1):813-821. Epub 2018 Sep 18.

Global Centre for Environmental Remediation (GCER), Faculty of Science, The University of Newcastle, Callaghan NSW 2308, Australia, and CRC CARE, Newcastle University LPO, PO Box 18, Callaghan, NSW 2308, Australia. Electronic address:

A Gram-positive bacterium, Rhodococcus wratislaviensis strain 9, completely degraded 280 μM of phenanthrene, 40% of 50 μM pyrene or 28% of 40 μM benzo[a]pyrene (BaP), each supplemented in M9 medium, within 7 days. PCR screening with gene-specific primers indicated that the strain 9 harbors genes which code for 2,3-dihydroxybiphenyl 1,2-dioxygenase (bphC), 4-nitrophenol 2-monooxygenase component B (npcB) as well as oxygenase component (nphA1), 4-hydroxybenzoate 3-monooxygenase (phbH), extradiol dioxygenase (edo), and naphthalene dioxygenase (ndo), all of which are largely implicated in biodegradation of several aromatic hydrocarbons. An orthogonal design experiment revealed that BaP biodegradation was greatly enhanced by surfactants such as Tween 80, Triton X-100 and linoleic acid, suggesting that bioavailability is the major limiting factor in bacterial metabolism of BaP. Read More

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February 2019
4 Reads

Molecular analysis of the chromosomal 16S rRNA gene and vapA plasmid gene of Polish field strains of R. equi.

PLoS One 2018 25;13(9):e0204024. Epub 2018 Sep 25.

Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.

Rhodococcus equi (R. hoagii) is an opportunistic pathogen commonly found in foals up to 6 months old and animal environment. The R. Read More

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September 2018
2 Reads

Virulence-associated protein A from Rhodococcus equi is an intercompartmental pH-neutralising virulence factor.

Cell Microbiol 2018 Sep 24:e12958. Epub 2018 Sep 24.

Division of Biophysics, Cell Biology Institute, University of Bonn, Bonn, Germany.

Professional phagocytic cells such as macrophages are a central part of innate immune defence. They ingest microorganisms into membrane-bound compartments (phagosomes), which acidify and eventually fuse with lysosomes, exposing their contents to a microbicidal environment. Gram-positive Rhodococcus equi can cause pneumonia in young foals and in immunocompromised humans. Read More

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September 2018
3 Reads

Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous.

Appl Microbiol Biotechnol 2018 Dec 22;102(23):10055-10065. Epub 2018 Sep 22.

Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, PO Wits 2050, South Africa.

Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase inducer, dimethylformamide (DMF), during cultivation of two Rhodococcus rhodochrous strains (ATCC BAA-870 and PPPPB BD-1780), as well as a closely related organism (Pimelobacter simplex PPPPB BD-1781). Benzonitrile, a known nitrilase inducer, was ineffective in these strains. Read More

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December 2018
1 Read

A novel dehydrogenase 17β-HSDx from Rhodococcus sp. P14 with potential application in bioremediation of steroids contaminated environment.

J Hazard Mater 2019 Jan 10;362:170-177. Epub 2018 Sep 10.

Department of Biology, Shantou University, Shantou, Guangdong, 515063, China. Electronic address:

Steroids are endocrine disrupting compounds in human and are distributed in various environments. Our previous study showed that a marine bacterium Rhodococcus sp. P14 was able to efficiently degrade one typical steroid estradiol. Read More

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January 2019
4.530 Impact Factor

Enantio- and regioselective ene-reductions using FH-dependent enzymes.

Chem Commun (Camb) 2018 Oct;54(79):11208-11211

Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

In the past decade it has become clear that many microbes harbor enzymes that employ an unusual flavin cofactor, the F420 deazaflavin cofactor. Herein we show that F420-dependent reductases (FDRs) can successfully perform enantio-, regio- and chemoselective ene-reductions. For the first time, we have demonstrated that F420H2-driven reductases can be used as biocatalysts for the reduction of α,β-unsaturated ketones and aldehydes with good conversions (>99%) and excellent regioselectivities and enantiomeric excesses (>99% ee). Read More

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October 2018
3 Reads
6.830 Impact Factor

Lignin depolymerization and utilization by bacteria.

Bioresour Technol 2018 Dec 30;269:557-566. Epub 2018 Aug 30.

Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, People's Republic of China. Electronic address:

Lignin compound wastes are generated as a result of agricultural and industrial practices. Microorganism-mediated bio-catalytic processes can depolymerize and utilize lignin eco-friendly. Although fungi have been studied since several decades for their ability to depolymerize lignin, strict growth conditions of fungus limit it's industrial application. Read More

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December 2018
1 Read

Intracellular Metabolic Changes of Rhodococcus sp. LH During the Biodegradation of Diesel Oil.

Mar Biotechnol (NY) 2018 Sep 14. Epub 2018 Sep 14.

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

In recent years, some marine microbes have been used to degrade diesel oil. However, the exact mechanisms underlying the biodegradation are still poorly understood. In this study, a hypothermophilous marine strain, which can degrade diesel oil in cold seawater was isolated from Antarctic floe-ice and identified and named as Rhodococcus sp. Read More

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September 2018

Bacterial conversion of depolymerized Kraft lignin.

Biotechnol Biofuels 2018 5;11:240. Epub 2018 Sep 5.

1Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.

Background: Lignin is a potential feedstock for microbial conversion into various chemicals. However, the degradation rate of native or technical lignin is low, and depolymerization is needed to obtain reasonable conversion rates. In the current study, base-catalyzed depolymerization-using NaOH (5 wt%)-of softwood Kraft lignin was conducted in a continuous-flow reactor system at temperatures in the range 190-240 °C and residence times of 1 or 2 min. Read More

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September 2018
8 Reads

Steroid Degradation in Comamonas testosteroni TA441: Identification of Metabolites and the Genes Involved in the Reactions Necessary before D-Ring Cleavage.

Appl Environ Microbiol 2018 Nov 30;84(22). Epub 2018 Oct 30.

Environmental Molecular Biology Laboratory, RIKEN, Saitama, Japan.

Bacterial steroid degradation has been studied mainly with () and as representative steroid degradation bacteria for more than 50 years. The primary purpose was to obtain materials for steroid drugs, but recent studies showed that many genera of bacteria (, , , etc.) degrade steroids and that steroid-degrading bacteria are globally distributed and found particularly in wastewater treatment plants, the soil, plant rhizospheres, and the marine environment. Read More

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November 2018
7 Reads

Fecal shedding of Rhodococcus equi in mares and foals after experimental infection of foals and effect of composting on concentrations of R. equi in contaminated bedding.

Vet Microbiol 2018 Sep 21;223:42-46. Epub 2018 Jul 21.

Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.

Rhodococcus equi, a soil saprophyte, is a common cause of pneumonia in foals and a frequent opportunistic pathogen in immunosuppressed people. Because it is widespread in the environment, R. equi can be detected in the feces of most horses. Read More

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September 2018
10 Reads