3,996 results match your criteria Reproductive BioMedicine Online [Journal]


The relative importance of genetic parenthood.

Reprod Biomed Online 2019 Mar 12. Epub 2019 Mar 12.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Amsterdam University Medical Center, University of Amsterdam Amsterdam, The Netherlands; Department of Development and Regeneration, KU Leuven-University of Leuven, Leuven, Belgium.

Research Question: How much do patients with severe infertility and their gynaecologists value genetic parenthood relative to other key treatment characteristics?

Design: A discrete choice experiment included the following treatment characteristics: genetic parenthood, pregnancy rate, curing infertility, maternal health, child health and costs. The questionnaire was disseminated between 2015 and 2016 among Dutch and Belgian patients with severe infertility and their gynaecologists.

Results: The questionnaire was completed by 173 patients and 111 gynaecologists. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.02.008DOI Listing

Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study.

Reprod Biomed Online 2019 Mar 12. Epub 2019 Mar 12.

Biology of the Testis Lab, Department of Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel (VUB), Brussels, Belgium.

Research Question: Does recombinant human vascular endothelial growth factor (VEGF-165) improve the efficiency of human immature testis tissue (ITT) xenotransplantation?

Design: ITT fragments from three prepubertal boys were cultured for 5 days with VEGF-165 or without (control) before xenotransplantation into the testes of immunodeficient mice. Xenotransplants were recovered at 4 and 9 months post-transplantation and vascularization, seminiferous tubule integrity, number of spermatogonia and germ cell differentiation were evaluated by histology and immunohistochemistry.

Results: Transplants from donor 1 and donor 2 treated with VEGF demonstrated higher vascular surface (P = 0. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.02.012DOI Listing

Mutational analysis of theFAM175A gene in patients with premature ovarian insufficiency.

Reprod Biomed Online 2019 Feb 28. Epub 2019 Feb 28.

Centre for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, China. Electronic address:

Research Question: The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause. However, it remains poorly understood whether sequence variants in FAM175A are causative for premature ovarian insufficiency (POI). The aim of this study was to investigate whether mutations in the gene FAM175A were present in patients with POI. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483193010
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http://dx.doi.org/10.1016/j.rbmo.2019.02.006DOI Listing
February 2019
1 Read

Follicle stimulating hormone or clomiphene citrate in intrauterine insemination with ovarian stimulation for unexplained subfertility: a role for treatment selection markers?

Reprod Biomed Online 2019 Mar 2. Epub 2019 Mar 2.

Centre for Reproductive Medicine, Academic Medical Centre, Amsterdam. Electronic address:

Research Question: Can women be identified, on the basis of baseline patient characteristics, as having better chances of an ongoing pregnancy with FSH instead of clomiphene citrate as stimulation agent in intrauterine insemination for unexplained subfertility?

Design: A secondary analysis of a multicentre randomized controlled superiority trial; the SUPER study. Between July 2013 and March 2016, couples with unexplained subfertility undergoing intrauterine inemination (IUI) were allocated to an FSH or clomiphene citrate group. Female age, body mass index, duration of subfertility, primary versus secondary subfertility, antral follicle count and total motile count were assessed. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.014DOI Listing

Association between FOXP3 regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts.

Reprod Biomed Online 2019 Jan 31. Epub 2019 Jan 31.

Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.

Research Question: Is there any relationship between numbers of FOXP3 regulatory T-cells (Treg) and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts?

Design: Retrospective and prospective case-controlled cohort study. Peritoneal lesions were collected from 27 women with ovarian endometrioma and 25 women with dermoid cysts. Peritoneal fluid was collected from 36 women with ovarian endometrioma and 42 women with dermoid cysts. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.011DOI Listing
January 2019
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Association between sperm DNA fragmentation and idiopathic recurrent pregnancy loss: a systematic review and meta-analysis.

Reprod Biomed Online 2018 Dec 22. Epub 2018 Dec 22.

Department of Obstetrics and Gynaecology, University of British Columbia and the Children's and Women's Hospital and Health Centre of British Columbia, Vancouver, BC, Canada. Electronic address:

Sperm DNA fragmentation (sDF) has emerged as a valuable tool for evaluating male fertility, yet the relationship between DNA fragmentation in the male gamete and idiopathic recurrent pregnancy loss (RPL) remains a topic of ongoing debate. Hence, a meta-analysis was conducted of 12 prospective and 2 retrospective studies involving 530 men with a history of RPL who underwent sDF testing compared with 639 fertile control participants. The main outcome measures were sDF measured by comet assay, TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL), sperm chromatin dispersion (SCD) or sperm chromatin structure assay. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.029DOI Listing
December 2018

Effects of hyperhomocysteinaemia and metabolic syndrome on reproduction in women with polycystic ovary syndrome: a secondary analysis.

Reprod Biomed Online 2019 Jan 18. Epub 2019 Jan 18.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China. Electronic address:

Research Question: What is the association between hyperhomocysteinaemia (HHCY), metabolic syndrome, and reproductive outcomes among women with polycystic ovary syndrome (PCOS).

Design: A secondary analysis of PCOSAct with 21 sites in China. A total of 1000 women with PCOS were enrolled; 936 women with baseline homocysteine (HCY) were analysed. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.046DOI Listing
January 2019
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Comparison of perinatal outcomes following blastocyst and cleavage-stage embryo transfer: analysis of 10 years' data from a single centre.

Reprod Biomed Online 2019 Jan 25. Epub 2019 Jan 25.

Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Centre, Xi'an, China. Electronic address:

Research Question: Are there greater risks of adverse perinatal outcomes, in particular of congenital malformations, after blastocyst transfer compared with cleavage-stage embryo transfer in IVF?

Design: This was a retrospective cohort analysis from a centre for assisted reproduction at a public hospital in China over the period 2006-2015. The analysis covered all women who conceived (15,254) and newborns (16,213) from IVF/intracytoplasmic sperm injection (ICSI) cycles with cleavage-stage embryo transfer or blastocyst transfer. The principal outcome measures were congenital malformations, preterm birth (PTB), low birthweight (LBW), small for gestational age (SGA) and large for gestational age (LGA). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.031DOI Listing
January 2019
2 Reads
3.015 Impact Factor

Human growth hormone for poor responders: a randomized placebo-controlled trial provides no evidence for improved live birth rate.

Reprod Biomed Online 2019 Feb 27. Epub 2019 Feb 27.

Monash IVF, Monash Surgical Private Hospital, Clayton, VIC 3168, Australia.

Research Question: Does the addition of human growth hormone (HGH) to an IVF cycle improve the live birth rate in previously documented poor responders to FSH?

Design: Double-blind, placebo-controlled, randomized clinical trial comparing HGH to placebo in maximal stimulation in an IVF cycle. The study was stopped after 4 years. Women receiving ovarian stimulation in one IVF cycle, having failed to produce more than 5 eggs in a previous cycle with more than 250 IU/day of FSH were included. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.02.003DOI Listing
February 2019

Live birth rate and neonatal outcome following cleavage-stage embryo transfer versus blastocyst transfer using the freeze-all strategy.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Zhizaoju Road No.639, Huangpu District, Shanghai 200011, People's Republic of China. Electronic address:

Research Question: What are the live birth rates and neonatal outcomes following cleavage-stage embryo transfer and blastocyst transfer in a freeze-all treatment scenario?

Design: This was a retrospective cohort study. All good-quality embryos were frozen on the third day; the remaining embryos were grown on until they reached blastocyst stage and then frozen. Between 2007 and 2016, 11,801 patients underwent cleavage-stage embryo transfer and 1009 patients underwent blastocyst transfer in the first treatment cycle using the freeze-all strategy. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.034DOI Listing
December 2018
3 Reads

Assessment of reflectance confocal microscopy for non-invasive selection of optimal ovarian cortex fragments for autotransplantation.

Reprod Biomed Online 2018 Dec 21. Epub 2018 Dec 21.

Department of Obstetrics and Gynecology, Radboud university medical center, PO Box 9101, Nijmegen HB 6500, The Netherlands.

Research Question: Can reflectance confocal microscopy (RCM) be used to determine follicle density in human ovarian cortex fragments that are intended for fertility restoration?

Design: RCM was used on living cortex tissue fragments derived from five bovine ovaries and 13 human ovaries. All tissue fragments were cryopreserved and thawed before RCM analysis. Follicle numbers and distribution were determined by RCM and histology. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.024DOI Listing
December 2018

Beneficial effects of dietary nitrate supplementation on testicular injury in streptozotocin-induced diabetic male rats.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Deptartment of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Research Question: Do low doses of dietary nitrate help to attenuate the progression of diabetic reproductive disorders in streptozotocin-induced diabetic male rats?

Design: Fifty male Wistar rats were divided into five groups: controls receiving distilled water; controls receiving 100 mg/l nitrate in distilled water; diabetic rats receiving distilled water; diabetic rats receiving insulin 2-4 U/day of neutral protamine hagedorn insulin; and diabetic rats receiving 100 mg/l nitrate in distilled water. Diabetes was induced by 45 mg/kg streptozotocin. Nitrate and insulin treatment were started 4 weeks after diabetes induction for 8 weeks. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.027DOI Listing
January 2019
3.015 Impact Factor

Association between progesterone to number of mature oocytes index and live birth in GnRH antagonist protocols.

Reprod Biomed Online 2019 Jan 23. Epub 2019 Jan 23.

Department of Reproductive Medicine, Toulouse University Hospital, Toulouse, France; EA 3694 Human Fertility Research Group, Paul Sabatier University, Toulouse, France.

Research Question: What is the association between blood progesterone level alone, progesterone to mature oocytes index (PMOI) and IVF outcomes?

Design: Clinical data from 960 couples undergoing their first fresh embryo transfer after an intracytoplasmic sperm injection attempt carried out between September 2012 and July 2017 were analysed. All patients underwent ovarian stimulation combining recombinant FSH and gonadotrophin releasing hormone antagonist. Progesterone was measured on the day on which ovulation was triggered. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.009DOI Listing
January 2019
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Follicular fluid progesterone concentration is associated with fertilization outcome after IVF: a systematic review and meta-analysis.

Reprod Biomed Online 2019 Jan 17. Epub 2019 Jan 17.

Obstetrics and Gynaecology, Medical School, University of Pécs, Hungary; MTA-PTE, Human Reproduction Scientific Research Group, Hungary.

Follicular fluid is a key biochemical environment for oocyte development. The potential effect of follicular progesterone level on successful fertilization is a subject of debate, and so the aim of this study was to provide a summary of the currently available evidence on the association between follicular fluid progesterone level and fertilization outcome. To do so, a systematic review and a meta-analysis were performed, with the literature searches being conducted in three databases (PubMed, Embase and the Cochrane Library) to identify all relevant studies published up to 19 August 2017. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.045DOI Listing
January 2019
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The high-risk human papillomavirus continuum along the female reproductive tract and its relationship to infertility and endometriosis.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Clinical Cytology and STD Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, PR, Brazil. Electronic address:

Research Question: Is there an association between the presence of sexually transmitted pathogens in the lower (LGT) and upper (UGT) female genital tract with endometriosis and infertility?

Design: Case-control study with 60 women submitted to gynaecological laparoscopic surgery. Samples from the UGT and LGT were collected and analysed by single polymerase chain reaction (PCR) for human papillomavirus (HPV) and by multiplex PCR for other sexually transmitted infections (STI). Patients were initially divided into two clinical groups: infertile patients (n = 25) with conjugal infertility and fertile control patients (n = 35). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.032DOI Listing
January 2019
3 Reads

A comparison of two months pretreatment with GnRH agonists with or without an aromatase inhibitor in women with ultrasound-diagnosed ovarian endometriomas undergoing IVF.

Reprod Biomed Online 2019 Apr 22;38(4):520-527. Epub 2018 Dec 22.

McGill University Health Centre Reproductive Centre, 888 de Maisonneuve East, Suite 200, Montreal Quebec H2L 4S8, Canada.

Research Question: Does the addition of an aromatase inhibitor improve IVF outcomes in women with endometriomas when pretreating them with gonadotrophin-releasing hormone agonists?

Design: Retrospective two-centre cohort study involving 126 women aged 21-39 years who failed a previous IVF cycle and all subsequent embryo transfers and had sonographic evidence of endometriomas. Women were non-randomly assigned to either 3.75 mg intramuscular depo-leuprolide treatment alone or in combination with 5 mg of oral letrozole daily for 60 days prior to undergoing a fresh IVF cycle. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183065
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http://dx.doi.org/10.1016/j.rbmo.2018.12.028DOI Listing
April 2019
12 Reads

A multicentre evaluation of the Elecsys anti-Müllerian hormone immunoassay for prediction of antral follicle count.

Reprod Biomed Online 2019 May 8;38(5):845-852. Epub 2019 Jan 8.

Heartland Center for Reproductive Medicine, 7308 South 142nd Street, Omaha NE 68138, USA.

Research Question: What concentration of anti-Müllerian hormone (AMH) corresponds to an antral follicle count (AFC) >15 for determination of ovarian reserve?

Design: A prospective study conducted at 13 US fertility clinics in women aged 21-44 years who presented for AFC evaluation by transvaginal ultrasound. Serum samples were collected at the time of AFC evaluation (menstrual cycle day 2-4). AMH concentrations were measured by the Elecsys AMH immunoassay; oestradiol and follicle-stimulating hormone (FSH) concentrations were also measured. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.041DOI Listing
May 2019
1 Read

Chief editor's 2018 annual report.

Reprod Biomed Online 2019 May 19;38(5):655-658. Epub 2019 Mar 19.

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http://dx.doi.org/10.1016/j.rbmo.2019.03.206DOI Listing

Consultation and treatment behaviour of infertile couples in China: a population-based study.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; National Clinical Research Centre for Obstetrics and Gynecology, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing 100191, China; Beijing Advanced Innovation Centre for Genomics, Beijing 100871, China; Peking-Tsinghua Centre for Life Sciences, Peking University, Beijing 100871, China. Electronic address:

Research Question: The prevalence of infertility in couples actively trying to conceive is 25%. What is the consultation-seeking behaviour, diagnosis and related treatment in infertile couples across China?

Design: Large cross-sectional population-based study in 2010-2011, in which 25,270 couples from eight provinces/municipalities in China were approached by a multistage stratified cluster sampling strategy.

Results: Among the 2680 couples reporting infertility, 1246 infertile couples consulted a fertility doctor. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.034DOI Listing
January 2019
3 Reads

Is polycystic ovary syndrome associated with risk of female sexual dysfunction? A systematic review and meta-analysis.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Department of Urology and Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong 510230, China. Electronic address:

The aim of this study was to evaluate whether polycystic ovary syndrome (PCOS) is a risk factor for female sexual dysfunction (FSD) by conducting a systematic review and meta-analysis. The databases PubMed, EMBASE and the Cochrane Library were searched for relevant studies. The association between PCOS and risk of FSD was assessed by relative risk or standard mean differences with 95% confidence interval. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.030DOI Listing
January 2019
1 Read

Analysis of chromosome microstructures in products of conception associated with recurrent miscarriage.

Reprod Biomed Online 2019 May 21;38(5):787-795. Epub 2018 Dec 21.

Department of Maternal-Fetal Biology, National Center Institute for Child Health and Development, Tokyo 157-8535, Japan. Electronic address:

Research Question: The causes of almost half of all miscarriages are unknown. Genetic alterations undetectable by conventional methods may cause some cases of recurrent miscarriage. The study aimed to identify candidate genetic alterations associated with recurrent miscarriage. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.010DOI Listing

Evaluation of the cost-effectiveness of ovulation suppression with progestins compared with GnRH analogs in assisted reproduction cycles.

Reprod Biomed Online 2019 May 12;38(5):691-698. Epub 2019 Jan 12.

Program in Reproductive Endocrinology and Gynecology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda MD, USA.

Research Question: Is ovulation suppression with progestins, requiring a freeze-all approach and subsequent frozen embryo transfer resulting from progestenic endometrial changes, cost-effective compared with gonadotropin releasing hormone analogues (GnRH) during assisted reproduction cycles.

Design: Cost-effectiveness analysis derived from a PubMed literature search of average US costs of GnRH agonist and antagonist IVF cycles.

Results: In all fresh IVF cycle models, progestin cycles were more expensive owing to the additional costs of increased gonadotropin use, embryo freezing and subsequent frozen embryo transfer (FET). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.044DOI Listing
May 2019
1 Read

Maternal β-HCG concentrations in early IVF pregnancy: association with the embryo development stage of blastocysts.

Reprod Biomed Online 2019 May 8;38(5):683-690. Epub 2019 Jan 8.

Department of Obstetrics & Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin Hong Kong, China. Electronic address:

Research Question: Does the blastocyst development stage on embryo transfer day affect serum β-human chorionic gonadotrophin (β-HCG) concentration 9 and 16 days after embryo transfer in women whose pregnancy progresses beyond 13 weeks?

Design: Retrospective study involving 204 cases where pregnancy progressed beyond 13 weeks of single blastocyst transfer in fresh (n = 66) or frozen embryo transfer (FET) (n = 138) cycles from January 2015 to September 2016.

Results: β-HCG concentrations 9 and 16 days after embryo transfer in FET cycles (median 223 IU/l and 4378 IU/l, respectively) were significantly (P < 0.001) higher than in fresh cycles (157 IU/l and 2813 IU/l). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.035DOI Listing

Larger oocyte cohorts maximize fresh IVF cycle birth rates and availability of surplus high-quality blastocysts for cryopreservation.

Reprod Biomed Online 2019 May 21;38(5):711-723. Epub 2018 Dec 21.

Shady Grove Fertility Reproductive Science Centre, Rockville MD, USA.

Research Question: How does oocyte cohort size affect IVF treatment outcomes?

Design: Retrospective cohort analysis of 10,193 fresh autologous oocyte retrievals among good-prognosis patients <35 years from 2009 to 2015. The primary outcome was live birth from a fresh transfer; secondary outcomes included cumulative live birth potential from the retrieved cohort and frequency of severe ovarian hyperstimulation syndrome (OHSS).

Results: Live birth per fresh transfer increased as the oocyte cohort increased up to 11-15 oocytes, then plateaued. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.007DOI Listing
May 2019
1 Read

Laser technology in the ART laboratory: a narrative review.

Reprod Biomed Online 2019 May 21;38(5):725-739. Epub 2018 Dec 21.

Nuffield Department of Women's and Reproductive Health, University of Oxford, John Radcliffe Hospital, Oxford, UK.. Electronic address:

To improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that the best quality gametes and embryos can be identified and manipulated, thus improving clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as assisted hatching, embryo biopsy, intracytoplasmic sperm injection cryopreservation and sperm immobilization/selection. This review examines the current status of cutting-edge laser-assisted reproductive technologies, investigates experimental techniques that are increasingly being applied clinically. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.011DOI Listing

Knowledge, attitudes and practice of physicians towards fertility and pregnancy-related issues in youngBRCA-mutated breast cancer patients.

Reprod Biomed Online 2019 May 10;38(5):835-844. Epub 2019 Jan 10.

Fertility Clinic, Research Laboratory on Human Reproduction, CUB-Erasme and Université Libre de Bruxelles, Brussels, Belgium.

Research Question: This study explored the knowledge, attitudes and practice of physicians towards fertility and pregnancy-related issues in young BRCA-mutated breast cancer patients.

Design: Physicians attending two international breast cancer conferences completed a 26-item questionnaire exploring fertility preservation, pregnancy during (BCP) or after breast cancer. A statistical comparison was carried out of the responses exploring the same issues in young breast cancer patients overall or specifically in those with BRCA mutations. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.031DOI Listing

Inferring biallelism of two FSH receptor mutations associated with spontaneous ovarian hyperstimulation syndrome by evaluating FSH, LH and HCG cross-activity.

Reprod Biomed Online 2019 May 23;38(5):816-824. Epub 2018 Dec 23.

Unit of Endocrinology, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; Centre for Genomic Research, University of Modena and Reggio Emilia, Modena, Italy. Electronic address:

Research Question: What is the cumulative effect of two follicle-stimulating hormone receptor (FSHR) mutations in spontaneous ovarian hyperstimulation syndrome (sOHSS) pathogenesis? Are these mutations in the mono- or biallelic state?

Design: Two FSHR mutations were found in a pregnant patient affected by sOHSS with no predisposing conditions. While the p.Asn106His mutation is novel, the p. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.021DOI Listing
May 2019
1 Read

'Is blood thicker than water?' Donor conceived offspring's subjective experiences of the donor: a systematic narrative review.

Reprod Biomed Online 2019 May 8;38(5):797-807. Epub 2019 Jan 8.

Department of Psychology, Family Studies and Research University Centre, Catholic University of Milan, Milan, Italy.

The aim of this study was to provide a systematic narrative review of the published research articles on donor conceived offspring's subjective experiences of their donor. A systematic search of electronic databases (PsycINFO and PubMed) provided 29 published quantitative and qualitative studies matching the inclusion criteria. The analysis of the studies outcomes led to the identification of four main themes: (i) views/representations of the donor; (ii) desire to contact the donor (in terms of desire to meet the donor, reasons for wanting to contact the donor, type and frequency of contact); (iii) reactions to discovering the donor's identity and contacting the donor; (iv) identity issues. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.033DOI Listing

NovelCFAP43 andCFAP44 mutations cause male infertility with multiple morphological abnormalities of the sperm flagella (MMAF).

Reprod Biomed Online 2019 May 23;38(5):769-778. Epub 2018 Dec 23.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; Anhui Province Key Laboratory of Reproductive Health and Genetics, Anhui Medical University, Hefei 230022, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230022, China. Electronic address:

Research Question: Multiple morphological abnormalities of the sperm flagella (MMAF) comprise a rare congenital disease that can cause primary male infertility. Several pathogenic genes (e.g. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.037DOI Listing

Absence of predictable long-term molecular effect of ulipristal acetate (UPA) on the endometrium.

Reprod Biomed Online 2019 May 21;38(5):825-834. Epub 2018 Dec 21.

Service de gynécologie obstétrique et médecine de la reproduction, Hôpital Tenon, AP-HP, Sorbonne Université, 4 rue de la Chine, Paris 75020, France; INSERM UMRS 938, Faculté de médecine Pierre et Marie Curie, Site Saint-Antoine, 27 rue Chaligny, PARIS cedex 12 75571, France. Electronic address:

Research Question: What are the effects of ulipristal acetate (UPA) on the expression of endometrial proliferation and maturation markers?

Design: A total of 45 endometrium-containing blocks of hysterectomy samples from non-menopausal women with a diagnosis of moderate to severe symptoms of uterine fibroids: 14 women operated on at the end of a 3-month course of UPA; four women who had discontinued UPA treatment 1-12 months before surgery; 27 control unexposed samples (14 in the proliferative and 13 in the secretory phase). Immunohistochemical staining of Ki67, vascular endothelial growth factor-receptor 2 (VEGFR2), oestradiol receptor, progesterone receptor, interleukin-15 (IL-15), indoleamin-2,3-dioxygenase (IDO) and C-C motif chemokine ligand-2 (CCL2) markers were analysed in both endometrial compartments and layers.

Results: Under UPA, oestradiol receptor and progesterone receptor expression is similar to the proliferative phase in both layers, although with a decrease in cell proliferation. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.013DOI Listing
May 2019
7 Reads

Evaluation of preimplantation genetic testing based on next-generation sequencing for balanced reciprocal translocation carriers.

Reprod Biomed Online 2019 May 8;38(5):669-675. Epub 2019 Jan 8.

Reproductive Medicine Centre, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing Jiangsu 210008, China; Reproductive Medicine Centre, Nanjing Drum Tower Hospital, The Affiliated Hospital to Nanjing University Medical School, Nanjing Jiangsu 210008, China. Electronic address:

Research Question: Can next-generation sequencing (NGS) based on copy number variation sequencing (CNV-Seq) identify normal/balanced embryos in balanced reciprocal translocation carriers and what are their reproductive outcomes?

Design: One hundred couples with balanced reciprocal translocation who underwent a total of 134 preimplantation genetic testing (PGT) cycles between January 2015 and October 2017 were evaluated. Trophectoderm cells of blastocysts were biopsied for CNV-Seq-based NGS. All the balanced/normal blastocysts were vitrified and cryopreserved. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.043DOI Listing
May 2019
1 Read

Early life environment influences the trajectory of post-partum weight loss in adult female rats.

Reprod Biomed Online 2019 May 12;38(5):779-786. Epub 2018 Dec 12.

University of Cambridge Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge CB2 0QQ, United Kingdom.

Research Question: The physiological processes of pregnancy and lactation require profound changes in maternal metabolism and energy balance. The timescale of metabolic reversion after pregnancy, in particular post-partum weight loss, is highly variable between individuals. Currently, mechanisms influencing post-partum metabolic recovery are not well understood. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.002DOI Listing
May 2019
2 Reads

Endometrial injury increases expression of hypoxia-inducible factor and angiogenesis in the endometrium of women with recurrent implantation failure.

Reprod Biomed Online 2019 May 22;38(5):761-767. Epub 2018 Dec 22.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China. Electronic address:

Research Question: The aim of this study was to compare expression of hypoxia-inducible factor 1-alpha (HIF-1α), angiogenesis and apoptosis in endometrial tissue near the implantation window of women with recurrent implantation failure (RIF) and in fertile control women, and to describe possible mechanisms of endometrial injury.

Design: A controlled clinical study was conducted. Endometrial tissue specimens were obtained from 20 women undergoing IVF who had had at least three previous failed treatment cycles; normal endometrial specimens were obtained from 10 fertile control women. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.027DOI Listing
May 2019
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Pituitary suppression protocol among Bologna poor responders undergoing ovarian stimulation using corifollitropin alfa: does it play any role?

Reprod Biomed Online 2018 Dec 25. Epub 2018 Dec 25.

Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium; University of Zagreb-School of Medicine, Department of Obstetrics and Gynecology Zagreb, Croatia. Electronic address:

Research Question: Does the type of pituitary suppression protocol influence cumulative live birth rate (LBR) in Bologna poor responders treated with corifollitropin alfa (CFA)?

Design: Retrospective cohort analysis including poor responder patients fulfilling the Bologna criteria who underwent their first intracytoplasmic sperm injection cycle using a CFA-based ovarian stimulation protocol between 2011 and 2017. The starting dose of CFA was 150 µg. The primary outcome was cumulative LBR, defined as the first delivery of a live born resulting from the fresh and all the subsequent frozen embryo transfers. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.030DOI Listing
December 2018

Vaginal microbiota profile at the time of embryo transfer does not affect live birth rate in IVF cycles with donated oocytes.

Reprod Biomed Online 2018 Dec 22. Epub 2018 Dec 22.

Clínica EUGIN, Barcelona 08029, Spain. Electronic address:

Research Question: What is the relationship between the vaginal microbiota profile at the time of embryo transfer and live birth rates in women undergoing IVF/intracytoplasmic sperm injection (ICSI) with donated oocytes?

Design: One hundred and fifty Caucasian women receiving donated oocytes were prospectively included in the study from March 2017 to January 2018. Samples of vaginal fluid were taken immediately before transfer of a fresh single blastocyst and genomic DNA (gDNA) was extracted. Bacterial load as well as the presence of four lactobacilli (L. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.019DOI Listing
December 2018
1 Read

Normalized second and fourth finger lengths in male and female partners and IVF cycle outcomes.

Reprod Biomed Online 2019 May 27;38(5):808-815. Epub 2019 Feb 27.

Universidad Rey Juan Carlos, Facultad de Ciencias de la Salud, Alcorcón (Madrid), Spain. Electronic address:

Research Question: Hox genes are involved in limb formation during normal embryological development. Their modulation by circulating maternal oestrogens and androgens determines the length of the second and fourth fingers of the adult hand. Do these same intrauterine hormone levels also determine fertility outcomes in the adult?

Design: To study the association between the length of the second and fourth fingers of both partners undergoing IVF (as a surrogate of their previous intrauterine exposure to oestrogens and androgens) with treatment outcome after IVF, data corresponding to 256 IVF cycles were analysed. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.02.001DOI Listing

Prolonged blastomere movement induced by the delay of pronuclear fading and first cell division adversely affects pregnancy outcomes after fresh embryo transfer on Day 2: a time-lapse study.

Reprod Biomed Online 2019 May 22;38(5):659-668. Epub 2018 Dec 22.

Kato Ladies Clinic, Tokyo 160-0023, Japan. Electronic address:

Research Question: What is the incidence, origin and clinical significance of blastomere movement after the first cell division in the human embryo?

Design: A total of 1096 embryos, cultured in the EmbryoScope+ ® time-lapse system and subjected to a single fresh cleaved embryo transfer, were retrospectively analysed. Type and duration of blastomere movement (dBMov) between the first (t2) and second cell division (t3) was monitored, and the ratio of dBMov during the 2-cell stage [dBMov/(t3-t2)] was calculated. Morphological evaluation of embryos was performed by referring to the size of the blastomere and fragmentation after first division in addition to Veeck's criteria on Day 2. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.014DOI Listing
May 2019
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Biological differences between focal and diffuse adenomyosis and response to hormonal treatment.

Reprod Biomed Online 2019 Apr 21;38(4):634-646. Epub 2018 Dec 21.

Department of Obstetrics and Gynecology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.

Research Question: Is there any difference in ovarian steroid receptor expression and pattern of fibrosis in focal and diffuse adenomyosis and response to hormonal treatment?

Design: Prospective controlled study where biopsy samples were prospectively collected after surgery from 30 women with focal adenomyosis, 21 women with diffuse adenomyosis and 20 women with uterine myoma. Some of these women underwent 3-6 months of treatment with gonadotrophin-releasing hormone agonist (GnRHa) before surgery. Tissue expression of oestrogen receptor (ER) and progesterone receptor (PR) was analysed by immunohistochemistry. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183064
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http://dx.doi.org/10.1016/j.rbmo.2018.12.015DOI Listing
April 2019
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Time-lapse imaging of inner cell mass splitting with monochorionic triamniotic triplets after elective single embryo transfer: a case report.

Reprod Biomed Online 2019 Apr 21;38(4):491-496. Epub 2018 Dec 21.

Assisted Conception Service, Glasgow Royal Infirmary, Glasgow, UK.

Research Question: Can detailed scrutiny of time-lapse imaging (TLI) of inner cell mass (ICM) splitting help to reduce the frequency of multiple pregnancies following elective single embryo transfer (eSET)?

Design: Retrospective analysis of time-lapse images of an embryo in vitro, which resulted in a monochorionic triamniotic pregnancy following eSET on Day 5 of development.

Results: A 37-year-old female patient underwent a frozen embryo transfer cycle whereby a single vitrified/warmed embryo was transferred at the hatching blastocyst stage. The subsequent pregnancy scan revealed a monochorionic triamniotic pregnancy. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.017DOI Listing
April 2019
2 Reads

Placental volume and other first-trimester outcomes: are there differences between fresh embryo transfer, frozen-thawed embryo transfer and natural conception?

Reprod Biomed Online 2019 Apr 23;38(4):538-548. Epub 2018 Dec 23.

Dijon University Hospital, Reproductive Biology Department, Dijon F-21000, France.

Research Question: Does mode of conception influence placental volume and other first-trimester outcomes?

Design: This retrospective single-centre case-control study led in Dijon University Hospital included 252 singleton pregnancies (84 IVF with either fresh embryo transfer or frozen-thawed embryo transfer [FET] and 168 natural conceptions). First-trimester placental volume, uterine artery pulsatility index and maternal serum PAPP-A and beta-HCG were measured. Statistical analyses were adjusted for gestational age, the newborn's gender, maternal age, parity, body mass index and smoking status. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.023DOI Listing
April 2019
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Maternal telomere length is shorter in intrauterine growth restriction versus uncomplicated pregnancies, but not in the offspring or in IVF-conceived newborns.

Reprod Biomed Online 2019 Apr 21;38(4):606-612. Epub 2018 Dec 21.

Telomere and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), 28029 Madrid, Spain.

Research Question: The study aimed to determine whether IVF or intrauterine growth restriction (IUGR) result in short neonatal telomeres, which could explain the higher risk of cardiovascular and metabolic disease described in these populations.

Design: This was an observational, analytical, cross-sectional, prospective study with controls in a tertiary hospital. The main outcome was to determine the leukocyte telomere length in 126 newborns and their mothers (n = 109). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.008DOI Listing

Pregnancy outcomes after recurrent pregnancy loss: a longitudinal cohort study on stress and depression.

Reprod Biomed Online 2019 Apr 20;38(4):599-605. Epub 2018 Dec 20.

Recurrent Pregnancy Loss Unit, Fertility Clinic 4071, University Hospital Copenhagen, Rigshospitalet, Blegdamsvej 9, Copenhagen Ø 2100, Denmark.

Research Question: Are self-reported symptoms of stress and depression associated with pregnancy outcomes within the first year after referral to a tertiary recurrent pregnancy loss unit?

Design: Prospective cohort study with online questionnaires using the Major Depression Inventory (MDI) and Cohen's Stress Scale (PSS) at referral and after 1 year. The study was conducted between 2010 and 2014. A total of 301 women who had experienced recurrent pregnancy loss completed the first questionnaire. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.006DOI Listing

Dual stimulation using corifollitropin alfa in 54 Bologna criteria poor ovarian responders - a case series.

Reprod Biomed Online 2019 May 22;38(5):677-682. Epub 2019 Jan 22.

The Fertility Clinic, Skive Regional Hospital, Denmark; Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark.

Research Question: What are the reproductive outcomes of Bologna criteria poor responders undergoing dual stimulation (DuoStim) and subsequent cryopreserved embryo transfer?

Design: Case series of patients treated during the period August 2015 to March 2018 in a public fertility clinic. The study included 54 Bologna criteria poor responder IVF patients younger than 42 years receiving a follicular stimulation (DuoStim 1) followed by a luteal phase stimulation (DuoStim 2) within the same cycle, both stimulations being performed with corifollitropin alfa followed by a subsequent cryopreserved embryo transfer cycle. The primary endpoint was the number of oocytes retrieved in DuoStim 1 compared with DuoStim 2. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.007DOI Listing
May 2019
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Changes in visual function and ocular morphology in women who have undergone ART treatment and children born as a result of ART treatment: a systematic review.

Reprod Biomed Online 2019 Apr 7;38(4):621-633. Epub 2018 Dec 7.

Department of Gynaecology and Obstetrics, Grande Ospedale Metropolitano of Reggio Calabria, Reggio Calabria, Italy. Electronic address:

As all the structures of the human eye are characterized by sex hormone receptors, this study tested the hypothesis that assisted reproductive technology (ART) treatment influences visual function and ocular morphology in women who have undergone ART treatment and children born as a result of ART treatment. A systematic literature search of all original articles published up to August 2018 was performed using the PubMed database, including all original studies available in the literature. Review articles, studies in which participants underwent mixed interventions (i. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.007DOI Listing
April 2019
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Two- and three-dimensional transvaginal ultrasonography for diagnosis of adenomyosis of the inner myometrium.

Reprod Biomed Online 2019 May 26;38(5):750-760. Epub 2018 Dec 26.

Department of Gynecology and Obstetrics, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, Aarhus 8200, Denmark.

Research Question: How diagnostically accurate is two-dimensional (2D-TVS) compared with three-dimensional transvaginal ultrasonography (3D-TVS) in diagnosing adenomyosis of the inner myometrium. What is the most accurate combination of ultrasonographic features?

Design: Premenopausal women (n = 110) scheduled for hysterectomy or transcervical resection of the endomyometrium owing to abnormal uterine bleeding were consecutively enrolled. All participants had real-time 2D-TVS and, later, blinded off-line 3D-TVS to diagnose adenomyosis. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.033DOI Listing
May 2019
2 Reads

The effect of different temperature conditions on human embryosin vitro: two sibling studies.

Reprod Biomed Online 2019 Apr 20;38(4):508-515. Epub 2018 Dec 20.

Universitair Ziekenhuis Brussel, Centre for Reproductive Medicine, Laarbeeklaan 101, 1090 Brussels, Belgium.

Research Question: What temperature is optimal for human embryo development up to day 5 or 6?

Design: Two prospective sibling oocyte studies on culture temperature were conducted in a university-based tertiary referral centre. Eligibility critera for both studies: Study 1: 50 cycles between August and October 2015, with culture at a stable temperature (37.0°C ± 0. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.020DOI Listing
April 2019
4 Reads

Evaluation of sperm telomere length in infertile men with failed/low fertilization after intracytoplasmic sperm injection.

Reprod Biomed Online 2019 Apr 23;38(4):579-587. Epub 2018 Dec 23.

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan Fertility and Infertility Centre, Isfahan, Iran. Electronic address:

Research Question: Telomeres are non-coding, repetitive DNA sequences (TTAGGG repeats) that play an important role in maintaining genome integrity. Unlike in somatic cells, telomere length in spermatozoa increases with male age and is considered as a molecular marker of sperm quality. The aetiology of failed fertilization following intracytoplasmic sperm injection (ICSI) is multifactorial; perhaps one of the reasons for this failure in these individuals is shortened sperm telomere length. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.022DOI Listing
April 2019
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Assessment of a double freezing approach in the management of surplus embryos in IVF.

Reprod Biomed Online 2019 Apr 8;38(4):517-519. Epub 2018 Dec 8.

Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Infertility and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan; Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation, Sackler Faculty of Medicine, Tel-Aviv University, Israel. Electronic address:

Research Question: What pregnancy rates are achieved after transfer of cryopreserved double slow-frozen embryos in IVF cycles? Patients in whom surplus thawed cleaved embryos (day 2 or 3) were grown to the blastocyst stage, re-frozen and then re-thawed for transfer (double freezing) were included.

Design: Data were collected on all patients who had undergone the above procedure at the IVF unit of Assuta Ramat Hachayal Hospital, Tel Aviv, during a 7-year period. For each patient in the study group, the two-consecutive, matched-by-age patients treated with frozen-thawed single blastocyst transfer were selected to form a 2:1 ratio control group. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.010DOI Listing

Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation.

Reprod Biomed Online 2019 Apr 22;38(4):570-578. Epub 2018 Dec 22.

Department of Obstetrics and Gynecology, Keio University Graduate School of Medicine, 35 Shinanomachi, -Shinjyuku-ku, Tokyo 160-8582, Japan.

Research Question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.026DOI Listing
April 2019
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Pro-endometriotic niche in endometriosis.

Reprod Biomed Online 2019 Apr 23;38(4):549-559. Epub 2018 Dec 23.

Department of Obstetrics and Gynaecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address:

Endometriosis is a complex and heterogeneous disorder of unknown aetiology. This benign disease possesses special biological behaviours that mimic those of malignant tumours. A pro-endometriotic niche established by an existing lesion is a supportive micro-environment for the progression of endometriosis. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.025DOI Listing
April 2019
2 Reads