3,951 results match your criteria Reproductive BioMedicine Online [Journal]


The effect of different temperature conditions on human embryosin vitro: two sibling studies.

Reprod Biomed Online 2018 Dec 20. Epub 2018 Dec 20.

Universitair Ziekenhuis Brussel, Centre for Reproductive Medicine, Laarbeeklaan 101, 1090 Brussels, Belgium.

Research Question: What temperature is optimal for human embryo development up to day 5 or 6?

Design: Two prospective sibling oocyte studies on culture temperature were conducted in a university-based tertiary referral centre. Eligibility critera for both studies: Study 1: 50 cycles between August and October 2015, with culture at a stable temperature (37.0°C ± 0. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.020DOI Listing
December 2018

Evaluation of sperm telomere length in infertile men with failed/low fertilization after intracytoplasmic sperm injection.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Department of Reproductive Biotechnology, Reproductive Biomedicine Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan Fertility and Infertility Centre, Isfahan, Iran. Electronic address:

Research Question: Telomeres are non-coding, repetitive DNA sequences (TTAGGG repeats) that play an important role in maintaining genome integrity. Unlike in somatic cells, telomere length in spermatozoa increases with male age and is considered as a molecular marker of sperm quality. The aetiology of failed fertilization following intracytoplasmic sperm injection (ICSI) is multifactorial; perhaps one of the reasons for this failure in these individuals is shortened sperm telomere length. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.022DOI Listing
December 2018

Assessment of a double freezing approach in the management of surplus embryos in IVF.

Reprod Biomed Online 2018 Dec 8. Epub 2018 Dec 8.

Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Infertility and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Ramat Gan; Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation, Sackler Faculty of Medicine, Tel-Aviv University, Israel. Electronic address:

Research Question: What pregnancy rates are achieved after transfer of cryopreserved double slow-frozen embryos in IVF cycles? Patients in whom surplus thawed cleaved embryos (day 2 or 3) were grown to the blastocyst stage, re-frozen and then re-thawed for transfer (double freezing) were included.

Design: Data were collected on all patients who had undergone the above procedure at the IVF unit of Assuta Ramat Hachayal Hospital, Tel Aviv, during a 7-year period. For each patient in the study group, the two-consecutive, matched-by-age patients treated with frozen-thawed single blastocyst transfer were selected to form a 2:1 ratio control group. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.010DOI Listing
December 2018

Distinctive subpopulations of the intestinal microbiota are present in women with unexplained chronic anovulation.

Reprod Biomed Online 2018 Dec 22. Epub 2018 Dec 22.

Department of Obstetrics and Gynecology, Keio University Graduate School of Medicine, 35 Shinanomachi, -Shinjyuku-ku, Tokyo 160-8582, Japan.

Research Question: Do gut microbiota associate with the ovulatory cycle in women showing normogonadotrophic anovulation? In humans, the gut microbiota affects diverse physiological functions and dysbiosis (microbial imbalance) may lead to pathological syndromes. However, there is comparatively little information on the relevance of gut microbiota to reproductive functions in women. Here, a group of women with idiopathic chronic anovulation were examined, who do not exhibit any apparent endocrinological disorder, as they are suitable for investigating the relationship between intestinal bacteria and ovulatory disorders. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.026DOI Listing
December 2018

Pro-endometriotic niche in endometriosis.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Department of Obstetrics and Gynaecology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. Electronic address:

Endometriosis is a complex and heterogeneous disorder of unknown aetiology. This benign disease possesses special biological behaviours that mimic those of malignant tumours. A pro-endometriotic niche established by an existing lesion is a supportive micro-environment for the progression of endometriosis. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.025DOI Listing
December 2018

The effect of serum vitamin D levels in couples on embryo development and clinical outcomes.

Reprod Biomed Online 2018 Dec 26. Epub 2018 Dec 26.

Reproductive Medicine Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Research Question: Do serum vitamin D levels in men and women undergoing IVF/intracytoplasmic sperm injection (ICSI) affect embryo development and clinical outcomes?

Design: A large retrospective observational cohort study conducted between March 2016 and March 2017; plasma 25-OHD levels were measured before ovarian stimulation in couples undergoing IVF/ICSI (including 1883 women and 1720 men).

Results: Women were divided into four groups in terms of serum 25-OHD quartiles: <14.8 ng/ml (Q1), 14. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.036DOI Listing
December 2018
1 Read

Hormonal medication in medically assisted reproduction: a systematic review of assessments from patients.

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

Centre for Reproductive Medicine, Women's and Children's Hospital, Academic Medical Centre, University of Amsterdam Amsterdam, the Netherlands; University of Leuven, Department of Development and Regeneration, Leuven, Belgium; University of Leuven, Department of Public Health and Primary Care, Leuven, Belgium; Research Foundation Flanders, Belgium. Electronic address:

Several hormonal fertility medications have comparable effectiveness. A literature review was conducted into patients' assessments regarding seven medication characteristics including 'side effects' and 'ease of use'. Medline, CINAHL and PsycINFO were searched for female fertility patients' written assessments of a hormonal medication. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.047DOI Listing
January 2019
1 Read

Effect of human embryonic morphological development on fetal growth parameters: the Rotterdam Periconceptional Cohort (Predict Study).

Reprod Biomed Online 2018 Dec 21. Epub 2018 Dec 21.

Department of Obstetrics and Gynaecology, Erasmus MC, University Medical Centre, PO Box 2040, Rotterdam 3000CA, the Netherlands. Electronic address:

Research Question: Is embryonic morphological development according to the Carnegie stages associated with pregnancy outcome?

Design: In a tertiary hospital-based cohort, 182 singleton non-malformed pregnancies were selected. Serial transvaginal three-dimensional ultrasound (3D-US) scans were carried out between 6 and 10 gestational weeks. Embryonic development was annotated according to the morphological criteria of the Carnegie classification using a virtual reality system. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.016DOI Listing
December 2018

Assessment of the calcium releasing machinery in oocytes that failed to fertilize after conventional ICSI and assisted oocyte activation.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium. Electronic address:

Research Question: Can oocyte-related activation deficiencies be evaluated in oocytes that failed to fertilize after intracytoplasmic sperm injection (ICSI) combined with assisted oocyte activation (AOA)?

Design: Evaluation of the spindle-chromosome complexes and intracellular distribution of inositol trisphosphate type 1 receptors (IP3R1) in in-vitro matured (IVM) and failed-to-fertilize oocytes from patients undergoing AOA. Assessment of the oocyte-related Ca releasing capacity in response to Ca ionophores and sperm microinjection in oocytes that failed to fertilize after ICSI or ICSI-AOA.

Results: IVM oocytes from patients undergoing conventional ICSI (control) and ICSI-AOA (study group) revealed a similar normalcy of spindle-chromosome complexes and distribution patterns of IP3R1. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.035DOI Listing
December 2018

Protective effect of estrogen receptor α-PvuII transition against idiopathic male infertility: A case-control study and meta-analysis.

Reprod Biomed Online 2019 Jan 23. Epub 2019 Jan 23.

Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran. Electronic address:

Research Question: Is there any genetic association between estrogen receptor alpha [ERα]-PvuII polymorphism and idiopathic male infertility?

Design: A total of 226 infertile and 213 fertile men participated in the present case-control study. ERα-PvuII genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism [PCR-RFLP] method. Meta-analysis was also performed by pooling data collected from seven other eligible studies identified by searches of PubMed, Embase, Google Scholar, and Science Direct databases. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.008DOI Listing
January 2019

Double stimulation in poor responders: a cause for concern?

Reprod Biomed Online 2019 Jan 18. Epub 2019 Jan 18.

King's College London NHS Foundation Trust, London, UK.

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http://dx.doi.org/10.1016/j.rbmo.2019.01.001DOI Listing
January 2019

Overnight ovarian tissue transportation for centralized cryobanking: a feasible option.

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

University Women's Hospital Bern, Division of Gynecological Endocrinology and Reproductive Medicine, Effingerstrasse 102, Bern 3010, Switzerland.

Research Question: Is overnight transportation of ovarian tissue before cryopreservation in a centralized cryobank from the FertiPROTEKT network feasible?

Design: Data from 1810 women with cryopreserved ovarian tissue after overnight transportation from December 2000 to December 2017 were analysed with a focus on transportation, tissue activity parameters and pregnancy, and delivery rates after transplantation.

Results: A total of 92.4% of tissue samples arrived at ideal temperatures of 2-8°C, 0. Read More

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http://dx.doi.org/10.1016/j.rbmo.2019.01.006DOI Listing
January 2019
1 Read

Prognostic value of repeated serum kisspeptin measurements in early first trimester pregnancy: a preliminary study.

Reprod Biomed Online 2018 Dec 7. Epub 2018 Dec 7.

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan Shandong 250012, People's Republic of China. Electronic address:

Research Question: What is the diagnostic value of maternal kisspeptin in patients with asymptomatic first-trimester pregnancies, and what is the prognostic significance of kisspeptin versus beta-HCG in early pregnancies.

Design: Case-control study in academic medical centres. Patients with no confounding co-morbidities who conceived by IVF and intracytoplasmic sperm injection were analysed. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.014DOI Listing
December 2018
1 Read

Individualization of the starting dose of follitropin delta reduces the overall OHSS risk and/or the need for additional preventive interventions: cumulative data over three stimulation cycles.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Ferring Pharmaceuticals, US Development, Parsippany, NJ 07054, USA.

Research Question: Is individualization of dosing with follitropin delta in sequential ovarian stimulation cycles an effective preventive strategy for ovarian hyperstimulation syndrome risk? If so, for which patients does an individualized strategy provide the greatest OHSS risk reduction and/or the need for additional preventive interventions?

Design: A secondary analysis of three ovarian stimulation cycles in IVF/intracytoplasmic sperm injection patients included in one randomized, assessor-blinded trial comparing two recombinant FSH preparations (ESTHER-1, NCT01956110), and a second trial in women undergoing up to two additional cycles (ESTHER-2, NCT01956123). Of 1326 women (aged 18-40 years) randomized and treated with follitropin delta or alfa in cycle 1, 513 continued to cycle 2 and 188 to cycle 3. Follitropin delta and alfa doses were maintained/adjusted according to ovarian response in the previous cycle. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.032DOI Listing
December 2018
1 Read

Live birth rates with a freeze-only strategy versus fresh embryo transfer: secondary analysis of a randomized clinical trial.

Reprod Biomed Online 2018 Dec 21. Epub 2018 Dec 21.

Department of Obstetrics and Gynaecology, School of Medicine, Monash University, Melbourne, Australia.

Research Question: What are the roles of serum progesterone and endometrial thickness as biomarkers in the decision between a freeze-only and fresh embryo transfer in IVF for women without polycystic ovary syndrome (PCOS)?

Design: This was a secondary analysis of a randomized controlled trial including 782 couples who were followed up until the end of the first completed cycle. Couples scheduled for their first or second IVF cycle with a FSH/gonadotrophin-releasing hormone antagonist protocol were randomized to a freeze-only (n = 391) or fresh embryo transfer (n = 391) strategy. The endpoint for this analysis was live birth rate (LBR) after the first embryo transfer. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.012DOI Listing
December 2018
1 Read

Meet our new editor: ProfessorFelice Petraglia.

Authors:

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

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http://dx.doi.org/10.1016/j.rbmo.2019.01.005DOI Listing
January 2019

Does theFMR1 gene affect IVF success?

Reprod Biomed Online 2018 Dec 10. Epub 2018 Dec 10.

AdvaGenix, Rockville MD, USA.

FMR1 CGG trinucleotide repeat expansions are associated with Fragile X syndrome (full mutations) and primary ovarian insufficiency (premutation range); the effect of FMR1 on the success of fertility treatment is unclear. The effect of FMR1 CGG repeat lengths on IVF outcomes after ovarian stimulation was reviewed. PubMed was searched for studies on IVF-related outcomes reported by FMR1 trinucleotide repeat length published between 2002 and December 2017. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.009DOI Listing
December 2018
1 Read

Variation analysis of theTMEM150B gene in Chinese women with premature ovarian insufficiency.

Reprod Biomed Online 2018 Dec 21. Epub 2018 Dec 21.

Centre for Reproductive Medicine, Shandong University, National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan 250021, China.

Research Question: The TMEM150B gene, which promotes cell survival under stress conditions by modulating autophagy, is closely associated with age at natural menopause, early menopause and premature ovarian insufficiency (POI) in European women. However, whether gene variants of TMEM150B contribute to the pathogenesis of POI needs to be determined.

Design: A case-control genetic study in 408 Han Chinese women with non-syndromic POI, in which all exons and exon-intron boundaries of the TMEM150B gene were screened by Sanger sequencing; the results were analysed by statistics and bioinformatics. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.009DOI Listing
December 2018
2 Reads

Bureaucratic overheating is a parasite hampering modern clinical research - a viewpoint from the 'belly of the beast'.

Reprod Biomed Online 2019 Jan 2. Epub 2019 Jan 2.

Department of Clinical Medicine, Aarhus University, Denmark; Fertility Clinic Skive, Skive Regional Hospital, Denmark.

Clinical researchers in Europe are experiencing an increasing amount of bureaucracy, which in our eyes has now reached surrealistic heights, hampering the future of research, and thus, the development of new treatment regimens and advances in clinical research. In the long term, the current approval and control processes will negatively impact patient care, education, academia and the health economy of our countries. Thus, a debate is necessary including not only healthcare workers, but also politicians and patients. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.024DOI Listing
January 2019

Regulating ART: time for a re-think in the light of increasing efficacy, safety and efficiency.

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK.

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http://dx.doi.org/10.1016/j.rbmo.2019.01.002DOI Listing
January 2019
3.015 Impact Factor

Novel inactivating mutations in the FSH receptor cause premature ovarian insufficiency with resistant ovary syndrome.

Reprod Biomed Online 2018 Dec 8. Epub 2018 Dec 8.

Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan 410078, People's Republic of China; Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan 410078, People's Republic of China; NHC Key Laboratory of human stem cell and reproductive engineering, Changsha, Hunan 410078, People's Republic of China. Electronic address:

Research Question: What is the genetic aetiology of three resistant ovary syndrome (ROS) pedigrees from 13 Chinese Han families with non-syndromic premature ovarian insufficiency (POI).

Design: The proband in each family was subjected to whole-exome sequencing. Bioinformatic and in-vitro functional analyses were performed for the functional characterization of the FSHR mutations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183059
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http://dx.doi.org/10.1016/j.rbmo.2018.11.011DOI Listing
December 2018
4 Reads

Meet our new editor: Professor Christopher Barratt.

Authors:

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

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http://dx.doi.org/10.1016/j.rbmo.2019.01.003DOI Listing
January 2019
1 Read

Meet our new editor: Professor Marinus JC Eijkemans.

Authors:

Reprod Biomed Online 2019 Jan 19. Epub 2019 Jan 19.

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http://dx.doi.org/10.1016/j.rbmo.2019.01.004DOI Listing
January 2019
1 Read

The IVF-LUBE trial - a randomized trial to assess Lipiodol uterine bathing effect in women with endometriosis or repeat implantation failure undergoing IVF.

Reprod Biomed Online 2018 Dec 7. Epub 2018 Dec 7.

Repromed Auckland and Auckland Gynaecology Group, 105 Remuera Road, Auckland, New Zealand; The Robinson Research Institute, School of Medicine, University of Adelaide, Australia; The University of Auckland, New Zealand.

Research Question: Does pre-IVF Lipiodol increase the success of IVF treatment in women with endometriosis or repeat implantation failure (RIF) compared with IVF alone?

Design: Lipiodol is known to enhance natural fertility, especially amongst women with endometriosis. The effect of Lipiodol may accrue through an impact on the endometrium that enhances receptivity to implantation. A randomized controlled trial (RCT) was carried out on 70 women due to undergo IVF. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.015DOI Listing
December 2018
1 Read

Hydrosalpinx and pregnancy loss: a systematic review and meta-analysis.

Reprod Biomed Online 2018 Dec 25. Epub 2018 Dec 25.

Tommy's National Centre for Miscarriage Research, Institute of Metabolism and Systems Research (IMSR), University of Birmingham, Academic Department, Birmingham Women's Hospital, Edgbaston, Birmingham B15 2TG, UK.

Research Question: What is the association between hydrosalpinx and pregnancy loss and the effects of treatment for hydrosalpinx?

Design: A systematic review and meta-analysis was conducted to investigate the risk of pregnancy loss, and the benefit of treatment in women with hydrosalpinx. Searches were conducted on MEDLINE, Embase, the Cochrane Library and Web of Science.

Results: A meta-analysis of 14 observational studies showed a 74% relative increase in the risk of pregnancy loss in women with hydrosalpinx compared with women without hydrosalpinx (relative risk [RR] = 1. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.020DOI Listing
December 2018
3 Reads

Obstetric and perinatal outcomes of pregnancies according to initial maternal serum HCG concentrations after vitrified-warmed single blastocyst transfer.

Reprod Biomed Online 2019 Jan 5. Epub 2019 Jan 5.

Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, Guangdong, People's Republic of China. Electronic address:

Research Question: Do pregnancy, obstetric and perinatal outcomes differ according to initial maternal serum human chorionic gonadotrophin (HCG) level measured on day 11 after single blastocyst transfer?

Design: Vitrified-warmed single blastocyst transfer cycles (n = 640) were collected between 1 January 2013 and 30 April 2017 with positive HCG values and retrospectively analysed by receiver operating characteristic curves to predict clinical pregnancy, ongoing pregnancy and delivery. Cycles were divided into a low HCG group (n = 155) and high HCG group (n = 485) based on cut-off value of live birth prediction. Cycles in the HCG group were subdivided into a low-high subgroup (n = 162), medium-high subgroup (n = 162) and high-high subgroup (n = 161) based on tertile points. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.040DOI Listing
January 2019
3 Reads

Autologous endometrial cell co-culture improves human embryo development to high-quality blastocysts: a randomized controlled trial.

Reprod Biomed Online 2019 Jan 5. Epub 2019 Jan 5.

Clinique Ovo, Ovo Labo, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; Clinique Ovo, Ovo R and D, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; Clinique Ovo, Ovo Fertility, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; University of Montréal, Department of Obstetrics and Gynecology, Saint-Luc Hospital (CHUM), 1051 Sanguinet Street, Montréal Quebec H2 × 0C1, Canada.

Research Question: Does autologous endometrial cell co-culture (AECC) improve the number of good-quality blastocysts obtained by IVF/intracytoplasmic sperm injection (ICSI), compared with conventional embryo culture medium in a broad group of patients referred to assisted reproductive technology (ART)?

Design: This interventional, randomized, double-blind study took place at Clinique Ovo from March 2013 to October 2015 and included 207 healthy patients undergoing an IVF or ICSI protocol, of which 71 were excluded before randomization. On the previous cycle, all participants underwent an endometrial biopsy at D5 to D7 post-ovulation, following which the endometrial cells were prepared for AECC.

Results: The data demonstrated that AECC significantly increased the incidence of good-quality blastocysts compared with culture in conventional media (42. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483193000
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http://dx.doi.org/10.1016/j.rbmo.2018.12.039DOI Listing
January 2019
4 Reads

Omega-3 polyunsaturated fatty acids and IVF treatment.

Reprod Biomed Online 2019 Jan 6;38(1):95-99. Epub 2018 Dec 6.

Department of Medical and Surgical Sciences, St. Orsola-Malpighi Hospital, University of Bologna 40138, Italy.

Omega-3 polyunsaturated fatty acids (PUFAs) are essential fatty acids, derived mostly from fish oil, that have a significant anti-inflammatory effect. Data from animal studies support their role in the reproductive mechanism, and recent human studies suggest a positive effect on sperm quality and natural conception. Their general role in human fertility, and specifically in IVF treatment, however, is not clear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183058
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http://dx.doi.org/10.1016/j.rbmo.2018.10.008DOI Listing
January 2019
5 Reads

Preconditioning of sperm with sublethal nitrosative stress: a novel approach to improve frozen-thawed sperm function.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR Tehran, Iran.

Research Question: Can sublethal stress induced by nitric oxide on fresh human spermatozoa protect the functional properties of post-thaw human spermatozoa?

Design: Semen samples were obtained from 46 donors. Twenty semen samples were used to determine toxicity level of nitric oxide by incubation of semen with different concentrations of nitric oxide (0.01 to 400 μM). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.029DOI Listing
January 2019
2 Reads

Acupuncture performed around the time of embryo transfer: a systematic review and meta-analysis.

Reprod Biomed Online 2019 Jan 2. Epub 2019 Jan 2.

Robinson Research Institute, University of Adelaide and Fertility SA, Adelaide SA 5000, Australia; Repromed Auckland, 105 Remuera Road, Auckland 1050, New Zealand.

This was a systematic review and meta-analysis to examine the efficacy, effectiveness and safety of acupuncture as an adjunct to embryo transfer compared with controls to improve reproductive outcomes. The primary outcome was clinical pregnancy. Twenty trials and 5130 women were included in the review. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.12.038DOI Listing
January 2019
5 Reads

C-type natriuretic peptide regulates sperm capacitation by the cGMP/PKG signalling pathway via Ca influx and tyrosine phosphorylation.

Reprod Biomed Online 2019 Jan 5. Epub 2019 Jan 5.

Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address:

Research Question: What is the effect of C-type natriuretic peptide (CNP) on human sperm capacitation in vitro and what is the mechanism of this effect?

Design: CNP/NPR-B expression in the female rat genital tract was examined by immunohistochemistry and western blot assay, and then the role of CNP in human sperm capacitation was determined. The signal transduction pathway of CNP in the process was determined to elucidate the regulation mechanism of CNP by enzyme-linked immunosorbent assay and flow cytometry.

Results: Both CNP and NPR-B were expressed in the genital tract of female rats, especially in the mucosa epithelium cell of the oviduct; the CNP level in the rat oviduct was higher than that in the cervix. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.025DOI Listing
January 2019
1 Read
3.015 Impact Factor

Reconceiving egg freezing: insights from an analysis of 5 years of data from a UK clinic.

Reprod Biomed Online 2019 Feb 11;38(2):272-282. Epub 2018 Dec 11.

London Women's Clinic, 113-115 Harley Street, London W1PG 6AP, UK.

Research Question: What can we learn from 5 years of egg-freezing practice in the UK? What are the different categories of egg freezing, and what are the social and demographic characteristics of patients, and their decisions regarding subsequent storage or thawing?

Design: A retrospective analysis of clinical and laboratory data of all 514 cycles of 'own' egg freezing conducted at the London Women's Clinic in the 5-year period from the start of 2012 to the end of 2016.

Results: This analysis, the first of its kind, develops a clearer picture of egg-freezing trends in the UK and fills in the details behind the Human Fertilisation and Embryology Authority's national figures. Four different categories of egg freezing are identified and the appropriate category allocated to each of the 514 cycles undertaken by 352 patients. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.003DOI Listing
February 2019
1 Read

Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes.

Reprod Biomed Online 2019 Jan 8. Epub 2019 Jan 8.

Instituto Valenciano de Infertilidad (IVI), University of Valencia, Pl. Policía Local 3, Valencia, Spain. Electronic address:

Research Question: Does the presence of dysmorphisms affect post-warming survival and embryo development in vitrified autologous oocytes?

Design: A retrospective study comparing post-warming survival, fertilization and embryo development between morphologically normal (n = 269) and dysmorphic oocytes (n = 147).

Results: The survival rate was 81.4% in the morphologically normal oocytes and 87. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.028DOI Listing
January 2019
1 Read

Germ cell arrest associated with aSETX mutation in ataxia oculomotor apraxia type 2.

Reprod Biomed Online 2019 Jan 9. Epub 2019 Jan 9.

Department of Obstetrics and Gynecology, Monash University, Clayton 3168, Melbourne, Australia; Monash IVF Group Pty Ltd, Richmond 3121, Melbourne, Australia.

Ataxia with oculomotor apraxia type 2 (AOA2) is a rare autosomal recessive neurodegenerative disorder characterized by cerebellar atrophy, peripheral neuropathy and oculomotor apraxia. It is caused by mutations in the SETX gene that encodes senataxin, a ubiquitously expressed protein that mediates processes, including transcription, transcription termination, DNA repair, RNA processing, DNA-RNA hybrid (R-loop) elimination and telomere stability. In mice, senataxin is essential for male germ cell development and fertility through its role in meiotic recombination and sex chromosome inactivation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483193001
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http://dx.doi.org/10.1016/j.rbmo.2018.12.042DOI Listing
January 2019
4 Reads

Serum progesterone concentration and live birth rate in frozen-thawed embryo transfers with hormonally prepared endometrium.

Reprod Biomed Online 2019 Jan 5. Epub 2019 Jan 5.

Service de Médecine de la Reproduction et Préservation de la Fertilité, Hôpital Jean-Verdier, Avenue du 14 Juillet, Bondy 93140, France.

Research Question: Is serum progesterone measurement on the day of embryo transfer associated with outcome of frozen-thawed embryo transfer (FET) in cycles using hormonal replacement therapy (HRT) for endometrium preparation?

Design: This single-centre retrospective study assessed the relationship between serum progesterone on embryo transfer day and live birth rates in 227 FET cycles. Endometrial preparation was performed by sequential administration of vaginal oestradiol until endometrial thickness was >7 mm, followed by transdermal oestradiol combined with 600 mg vaginal micronized progesterone.

Results: Mean serum embryo transfer day progesterone was 11. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.026DOI Listing
January 2019
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Incidence and origin of meiotic whole and segmental chromosomal aneuploidies detected by karyomapping.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Repromeda, Clinic for Reproductive Medicine and Preimplantation Genetic Diagnosis, Biology Park, Studentská 812/6, 625 00 Brno, Czech Republic.

Research Question: What is the incidence and origin of meiotic whole and segmental aneuploidies detected by karyomapping at a blastocyst stage in human-derived IVF embryos? What is the distribution of various types of errors, including rare chromosomal abnormalities?

Design: The incidence of chromosomal aneuploidies was assessed in 967 trophectoderm biopsies from 180 couples who underwent 215 cycles of IVF with preimplantation genetic testing for monogenetic disease with a known causal mutation with a mean maternal age of 32.7 years. DNA from both parents and a reference sample was genotyped together with the analysed trophectoderm samples by karyomapping (single-nucleotide-polymorphism-based array). Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.023DOI Listing
December 2018
2 Reads

Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model.

Reprod Biomed Online 2018 Dec 8. Epub 2018 Dec 8.

Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Federal University of Uberlândia, 38400-902, Uberlândia, Minas Gerais, Brazil. Electronic address:

Research Question: Does a three-dimensional culture system based on magnetic levitation with nanoparticles assembly maintain the follicular structure and viability with adequate growth rates leading to oocyte maturation after long-term culture?

Design: Randomized-controlled trial of treatments in a bovine model. Secondary follicles (n = 213) isolated from bovine ovaries were cultured in a two-dimensional system (two-dimensional control) or three-dimensional levitation system with different concentrations (three-dimensional 50 µl/ml, 100 µl/ml and 200 µl/ml) of magnetic nanoparticles. Follicular growth (diameter, daily growth and growth patterns), morphology (normal, degenerated and extruded follicles), antrum formation, oocyte viability and chromatin configuration were assessed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183059
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http://dx.doi.org/10.1016/j.rbmo.2018.11.013DOI Listing
December 2018
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Live births following genome editing in human embryos: a call for clarity, self-control and regulation.

Reprod Biomed Online 2019 Feb 14;38(2):131-132. Epub 2018 Dec 14.

ART Institute of Washington, Inc. Electronic address:

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http://dx.doi.org/10.1016/j.rbmo.2018.12.003DOI Listing
February 2019
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Choosing an expanded carrier screening panel: comparing two panels at a single fertility centre.

Reprod Biomed Online 2019 Feb 14;38(2):225-232. Epub 2018 Dec 14.

Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Northwell Health, 300 Community Dr, Manhasset NY 11030, USA; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, 500 Hofstra Blvd, empstead NY 11549, USA. Electronic address:

Research Question: What are the factors contributing to similarities and differences in carrier rates between two expanded carrier screening (ECS) panels?

Design: Retrospective cross-sectional study. A total of 7700 infertility patients who underwent ECS from one of two genetic testing laboratories (Panel A or Panel B) using a genotyping microarray were included in the study. Individuals presenting to the Centre between June 2013 and July 2015 underwent screening via Panel A. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.018DOI Listing
February 2019
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Endometrial stromal cell proteome mapping in repeated implantation failure and recurrent pregnancy loss cases and fertile women.

Reprod Biomed Online 2018 Dec 23. Epub 2018 Dec 23.

Department of Reproductive Medicine, Ghent Fertility and Stem Cell Team (G-Fast), Ghent University Hospital, Corneel Heymanslaan 10, Ghent 9000, Belgium.

Research Question: Are there proteomic differences between endometrial stromal cells of repeated implantation failure (RIF), recurrent pregnancy loss (RPL) and normal fertile women, and is there differential protein expression upon decidualization?

Design: This exploratory study investigated the proteome of in-vitro cultured endometrial stromal cells of women with RIF (n = 4), women with RPL (n = 3) and normal fertile women (n = 4), comparing day 0 with 5 days of decidualization. Total proteins extracted from cell lysates were analysed by high-definition mass spectrometry. Data analysis was performed using significance analysis of microarray in R (P < 0. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.022DOI Listing
December 2018
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The use of home remedies and complementary health approaches in endometriosis.

Reprod Biomed Online 2019 Feb 14;38(2):260-271. Epub 2018 Dec 14.

University Hospital Zürich, Department of Reproductive Endocrinology, Zürich 8910, Switzerland. Electronic address:

Research Question: Conventional treatments are often associated with adverse effects and endometriosis pain symptoms may reoccur despite treatment. Consequently, many women use complementary health approaches (CHA) and home remedies (HR) to relieve their pain. The aim of this study was to examine the frequency and the subjectively perceived efficacy of CHA/HR use by women affected by endometriosis. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.10.009DOI Listing
February 2019
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Ovarian stimulation and endometriosis progression or recurrence: a systematic review.

Reprod Biomed Online 2019 Feb 23;38(2):185-194. Epub 2018 Dec 23.

Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy; Department of Obstetrics and Gynecology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.

Available evidence on the impact of ovarian stimulation on the progression of endometriosis or its recurrence was systematically reviewed. Data from ovarian stimulation alone, or associated with intrauterine insemination (IUI) or IVF, were included. Sixteen studies were selected. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.021DOI Listing
February 2019
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Soluble LH-HCG receptor and oestradiol as predictors of pregnancy and live birth in IVF.

Reprod Biomed Online 2019 Feb 15;38(2):159-168. Epub 2018 Dec 15.

Origin Biomarkers, Biocity Scotland, B'oness Road, Newhouse, Lanarkshire ML1 5UH, UK.

Research Question: Circulating soluble LH-HCG receptor (sLHCGR) is a first-trimester marker for screening pregnancy pathologies and predicts premature or multiple births before fertility treatment. Oestradiol per oocyte at ovulation induction predicts IVF treatment outcomes. We asked whether sLHCGR levels are stable during fertility treatment and whether, alone or with oestradiol, they could improve prediction of fertility treatment outcomes. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.019DOI Listing
February 2019
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Dydrogesterone: pharmacological profile and mechanism of action as luteal phase support in assisted reproduction.

Reprod Biomed Online 2019 Feb 15;38(2):249-259. Epub 2018 Dec 15.

Department of Reproductive Medicine and Gynecology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.

The pharmacological and physiological profiles of progestogens used for luteal phase support during assisted reproductive technology are likely to be important in guiding clinical choice towards the most appropriate treatment option. Various micronized progesterone formulations with differing pharmacological profiles have been investigated for several purposes. Dydrogesterone, a stereoisomer of progesterone, is available in an oral form with high oral bioavailability; it has been used to treat a variety of conditions related to progesterone deficiency since the 1960s and has recently been approved for luteal phase support as part of an assisted reproductive technology treatment. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.017DOI Listing
February 2019
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3.015 Impact Factor

Follitropin delta in repeated ovarian stimulation for IVF: a controlled, assessor-blind Phase 3 safety trial.

Reprod Biomed Online 2019 Feb 14;38(2):195-205. Epub 2018 Dec 14.

Reproductive Health, Ferring Pharmaceuticals, Denmark.

Research Question: To evaluate the immunogenicity of follitropin delta in repeated ovarian stimulation.

Design: Controlled, assessor-blind trial in IVF/intracytoplasmic sperm injection patients undergoing repeated cycles of ovarian stimulation (cycles 2 and 3), following initial stimulation with follitropin delta or follitropin alfa (cycle 1) in a preceding randomized trial. In cycles 2 and 3, 513 and 188 women, respectively, were treated as randomized in cycle 1, with dosing based on ovarian response in the previous cycle. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.10.012DOI Listing
February 2019
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Towards the global coverage of a unified registry of IVF outcomes.

Authors:
Bart Cjm Fauser

Reprod Biomed Online 2019 Feb 14;38(2):133-137. Epub 2018 Dec 14.

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http://dx.doi.org/10.1016/j.rbmo.2018.12.001DOI Listing
February 2019
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Association between early embryo morphokinetics plus cumulus cell gene expression and assisted reproduction outcomes in polycystic ovary syndrome women.

Reprod Biomed Online 2019 Feb 14;38(2):139-151. Epub 2018 Dec 14.

Research and Clinical Centre for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Electronic address:

Research Question: Can a combination of time-lapse morphokinetic parameters and cumulus cell gene expression in polycystic ovary syndrome (PCOS) women be used to predict assisted reproductive treatment outcome?

Design: A total of 547 embryos from 100 intracytoplasmic sperm injection (ICSI) cycles were evaluated. Fifty women with PCOS and 50 women who were categorized as tubal factor infertility were recruited. Time-lapse records were annotated for time to pronuclear fading (tPNf), time to 2 to 8 cells (t2-t8), reverse cleavage, direct cleavage and also for the presence of multinucleation. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.10.010DOI Listing
February 2019
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Evaluating the benefit of measuring serum progesterone prior to the administration of hCG: effect of the duration of late-follicular elevated progesterone following ovarian stimulation on fresh embryo transfer live birth rates.

Reprod Biomed Online 2018 Dec 8. Epub 2018 Dec 8.

Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, Brussels 1090, Belgium; Department of Obstetrics and Gynaecology, School of Medicine, University of Zagreb, Šalata 3, Zagreb 10000, Croatia.

Research Question: progesterone (P) overproduction during ovarian stimulation is associated with lower live-birth rates (LBR) after fresh embryo transfer. Therefore, circulating P levels on the day of hCG administration are frequently measured in clinical practice and followed by an elective cryopreservation strategy whenever late-follicular elevated P (LFEP) occurs. A recent study concluded that the duration of LFEP >1. Read More

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http://dx.doi.org/10.1016/j.rbmo.2018.11.016DOI Listing
December 2018
1 Read

Low MFN2 expression related to ageing in granulosa cells is associated with assisted reproductive technology outcome.

Reprod Biomed Online 2019 Feb 14;38(2):152-158. Epub 2018 Dec 14.

Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, China; Centre of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address:

Research Question: Is low MFN2 expression associated with ageing in granulosa cells as well as assisted reproductive technology (ART) outcome, and what is the underlying mechanism of action of MFN2?

Design: In a prospective study, fresh granulosa cells were obtained from 161 women aged 20-40 years who underwent IVF with embryo transfer and who were divided into two groups: the diminished ovarian reserve (DOR) group (n = 51) and the control group (n = 110). Patient characteristics including age, infertility duration, body mass index, FSH, anti-Müllerian hormone (AMH), antral follicle count (AFC) and husband's semen parameters and granulosa cell MFN2 expression levels, cell apoptosis, mitochondrial membrane potential (ΔΨm) and ATP levels were analysed.

Results: There were no significant differences between the DOR and control groups in terms of age, infertility duration and husband'' semen parameters; however, significant (P< 0. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S14726483183060
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http://dx.doi.org/10.1016/j.rbmo.2018.10.011DOI Listing
February 2019
9 Reads