3,274 results match your criteria Reproduction[Journal]


Discovering Science and the Ovary: A Career of Joy.

Authors:
JoAnne Richards

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

J Richards, Molecular and Cellular Biology, Baylor College of Medicine, Houston, United States.

My career has been about discovering science and learning the joys of the discovery process itself. It has been a challenging but rewarding process filled with many exciting moments and wonderful colleagues and students. Although I went to college to become a French major, I ultimately stumbled into research while pursuing a Masters Degree in teaching. Read More

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http://dx.doi.org/10.1530/REP-18-0513DOI Listing
February 2019

The contribution of epididymosomes to the sperm small RNA profile.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

B Nixon, School of Environmental and Life Sciences, The University of Newcastle, Priority Research Centre for Reproductive Science, Callaghan, Australia.

It is now well established that mature spermatozoa harbour a rich and diverse profile of small non-protein coding regulatory RNAs (sRNAs). There is also growing appreciation that this sRNA profile displays considerable plasticity, being altered in response to paternal exposure to a variety of environmental stressors. Coupled with evidence that upon delivery to the oocyte at the moment of fertilisation, sperm-borne sRNAs are able to influence both early embryonic development and the subsequent health of the offspring, there is now interest in both the timing and degree of change in the composition of the sRNA cargo of sperm. Read More

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http://dx.doi.org/10.1530/REP-18-0480DOI Listing
February 2019

H19 regulates trophoblastic spheroid adhesion by competitively binding to let-7.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

N Liu, reproductive center of medicine, Xiangya Hospital Central South University, Changsha, China.

Integrin β3 (ITGB3), which is the target gene of the miRNA let-7 that can be antagonized by long noncoding RNA (lncRNA) H19, is well-known to have a critical role in endometrium receptivity. However, the regulation of ITGB3 in cell-cell or cell-extracellular matrix adhesion and invasion for the maintenance of early pregnancy remains unknown. This study aimed to explore the role of the H19/let-7/ITGB3 axis in regulating trophoblastic spheroid adhesion and in vitro invasion ability using the HTR-8/SVneo cell line and to investigate the expression levels of lncRNA H19 and ITGB3 in human products of conception. Read More

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http://dx.doi.org/10.1530/REP-18-0339DOI Listing
February 2019

Progesterone alters the bovine uterine fluid lipidome during the period of elongation.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

P Lonergan, School of Agriculture and Food Science, University College Dublin, Dublin 4, Ireland.

Successful bovine pregnancy establishment hinges on conceptus elongation, a key reproductive phenomenon coinciding with the period during which most pregnancies fail. Elongation has yet to be recapitulated in vitro whereas in vivo it is directly driven by uterine secretions and indirectly influenced by prior circulating progesterone levels. To better understand the microenvironment evolved to facilitate this fundamental developmental event, uterine fluid was recovered on Days 12-14 of the estrous cycle - the window of conceptus elongation initiation - from cycling heifers supplemented, or not, with progesterone. Read More

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http://dx.doi.org/10.1530/REP-18-0615DOI Listing
February 2019

Cyclin-dependent kinase 21 is a novel regulator of proliferation and meiosis in the male germ line of zebrafish.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

K Siegfried, Biology Department, University of Massachusetts Boston, Boston, 02125, United States.

Germ cell differentiation and maintenance relies on complex regulation of mitotic and meiotic progression. Cyclin-dependent kinases (Cdks) and their activating Cyclin partners are known to have specialized roles in regulating cell-cycle progression across tissues, including in germ cells. Very little is known about Cdk/Cyclin function in zebrafish, or the regulation of germ cell maintenance and differentiation. Read More

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http://dx.doi.org/10.1530/REP-18-0386DOI Listing
February 2019

Serum and tissue pregnanes and pregnenes after dexamethasone treatment of cows in late gestation.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

L Reynolds, Department of Animal Sciences, North Dakota State University, Fargo, United States.

Dexamethasone (DEX) initiates parturition by inducing progesterone withdrawal and affecting placental steroidogenesis, but the effects of DEX in fetal and maternal tissue steroid synthetic capacity remains poorly investigated. Blood was collected from cows at 270 days of gestation before DEX or saline (SAL) treatment, and blood and tissues were collected at slaughter 38 hours later. Steroid concentrations were determined by liquid chromatography tandem mass spectrometry to detect multiple steroids including 5α-reduced pregnane metabolites of progesterone. Read More

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http://dx.doi.org/10.1530/REP-18-0558DOI Listing
February 2019

New insights of the role of β-NGF in the ovulation mechanism of induced ovulating species.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

G Adams, Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.

The type of stimuli triggering GnRH secretion has been used to classify mammalian species into two categories: spontaneous or induced ovulators. In the former, ovarian steroids produced by a mature follicle elicit the release of GnRH from the hypothalamus, but in the latter, GnRH secretion requires coital stimulation. However, the mechanism responsible for eliciting the preovulatory LH surge in induced ovulators is still not well understood and seems to vary among species. Read More

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http://dx.doi.org/10.1530/REP-18-0305DOI Listing
February 2019

Notch activity mediates oestrogen-induced stromal cell invasion in endometriosis.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

W Xiong, Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wu Han, China.

Oestrogen has been reported to control the invasiveness of endometrial stromal cells in endometriosis. Notch signalling, a master regulator of cell invasion in tumours, is regulated by oestrogen in other diseases and hyperactivated in endometriotic stromal cells. Therefore, we hypothesized that an interaction between Notch signalling and oestrogen may exist in the regulation of endometrial stromal cell invasion, which is essential for the development of endometriosis. Read More

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https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-
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http://dx.doi.org/10.1530/REP-18-0326DOI Listing
February 2019
2 Reads
3.174 Impact Factor

DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos.

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

Z Wu, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Somatic cell nuclear transfer in mammalian cloning suffers from a faulty epigenetic reprogramming, which is believed to cause developmental failures in cloned embryos. Regulating the epigenetic-modifying enzymes can rescue the chromatin of cloned embryos from aberrant epigenetic status, thereby potentially promoting cloning efficiency. In this study, we investigated the effect of two histone methyltransferase inhibitors, namely, DZNep and UNC0642, on the in vitro developmental competence of cloned pig embryos. Read More

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http://dx.doi.org/10.1530/REP-18-0571DOI Listing
February 2019

Update on mammalian sperm capacitation: How much does the horse differ from other species?

Reproduction 2019 Feb 1. Epub 2019 Feb 1.

B Gadella, Faculty of Veterinary Medicine Department Bicohemistry and Cell Biology, Utrecht University, Utrecht, Netherlands.

In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fail to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) under in vitro conditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Read More

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http://dx.doi.org/10.1530/REP-18-0541DOI Listing
February 2019
3.174 Impact Factor

OVARIAN STIMULATION FOR PREIMPLANTATION GENETIC TESTING.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

F Martinez, Reproductive Medicine Service, Hospital Universitari Dexeus, Barcelona, Spain.

A narrative review of the management of controlled ovarian stimulation in patients undergoing preimplantation genetic testing is presented. An electronic search was performed to identify research publications that addressed ovarian stimulation and preimplantation genetic testing published until December 2017. Studies were classified in decreasing categories: randomized controlled trials, prospective controlled trials, prospective non-controlled trials, retrospective studies, and experimental studies. Read More

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https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-
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http://dx.doi.org/10.1530/REP-18-0475DOI Listing
January 2019
3 Reads

Do Prenatal Exposures Pose a Real Threat to Ovarian Function? BPA as a Case Study.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

S Mahalingaiah, Department of Obstetrics and Gynecology, Boston Medical Center/Boston University School of Medicine, Boston, United States.

Fetal development represents a time of potential vulnerability due to rapid cell division, organ development, and limited fetal kidney/liver activity for detoxification and metabolism of exposures. Health effects of prenatal toxicant exposure have previously been described, but there is little cohesive evidence surrounding effects on ovarian function. Using bisphenol A (BPA) as a case study, we seek to examine whether a prominent prenatal environmental exposure can pose a real threat to human ovarian function. Read More

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http://dx.doi.org/10.1530/REP-17-0734DOI Listing
January 2019
1 Read

Ejaculate-mediated paternal effects: evidence, mechanisms and evolutionary implications.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

F Garcia-Gonzalez, ., Estacion Biologica de Donana CSIC, Sevilla, Spain.

Despite serving the primary objective of ensuring that at least one sperm cell reaches and fertilizes an ovum, the male ejaculate (i.e. spermatozoa and seminal fluid) is a compositionally complex 'trait' that can respond phenotypically to subtle changes in conditions. Read More

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http://dx.doi.org/10.1530/REP-18-0524DOI Listing
January 2019
1 Read

Evidence that growth hormone can improve mitochondrial function in oocytes from aged mice.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

Y Qin, Obstetrics and Gynecology, Characteristic Medical Center of PAP, Tianjin, China.

Decline in successful conception decreases more rapidly after 38 years of age owing to follicular depletion and decreased oocyte quality. However, limited information is available regarding the underlying mechanism and the useful treatment. This study aimed to evaluate the effects of growth hormone supplementation on oocyte maturation in vivo in aged and young mice and to determine its effect on mitochondrial function. Read More

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http://dx.doi.org/10.1530/REP-18-0529DOI Listing
January 2019
1 Read
3.174 Impact Factor

Adverse reproductive outcomes associated with fetal alcohol exposure: A systematic review.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

N Reid, Child Health Research Centre, University of Queensland, South Brisbane, Australia.

Fetal alcohol exposure results in well-characterised neuro-behavioural deficits in offspring, which form the basis for diagnosing fetal alcohol spectrum disorder. However, there is increasing interest in the full range of health complications that can arise in children and adults with this disorder. We used a systematic review approach to locate all clinical and preclinical studies across a broad range of health outcomes in offspring exposed to prenatal alcohol. Read More

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https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-
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http://dx.doi.org/10.1530/REP-18-0607DOI Listing
January 2019
6 Reads

Association of foetal size and sex with porcine feto-maternal interface integrin expression.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

C Ashworth, Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom of Great Britain and Northern Ireland.

Integrins regulate adhesion at the feto-maternal interface by interacting with secreted phosphoprotein 1 (SPP1) and fibronectin (FN). It is hypothesised that impaired foetal growth of 'runt' piglets is linked to altered integrin signalling at the feto-maternal interface. Placental and endometrial samples associated with the lightest and closest to mean litter weight (CTMLW) (gestational day (GD18, 30, 45, 60 and 90), of both sex (GD30, 45, 60 and 90) (n=5-8 litters/GD), Large White X Landrace conceptuses or foetuses were obtained. Read More

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https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-
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http://dx.doi.org/10.1530/REP-18-0520DOI Listing
January 2019
5 Reads

Oocyte-specific deletion of Hdac8 in mice reveals stage-specific effects on fertility.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

F Duncan, Obstetrics and Gynecology, Northwestern University, Chicago, United States.

Eighteen histone deacetylases exist in mammals. The class 1 histone deacetylases HDAC1 and HDAC2 are important for oogenesis and fertility in mice, likely via their effects on histones. The reproductive function of HDAC8, another class 1 enzyme, has not been explored. Read More

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http://dx.doi.org/10.1530/REP-18-0560DOI Listing
January 2019
1 Read

Regulation of GDNF expression in Sertoli cells.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

M Hofmann , Endocrine Neoplasia and Hormonal Disorders, University of Texas MD Anderson Cancer Center, Houston, United States.

Sertoli cells regulate male germ cell proliferation and differentiation and are a critical component of the spermatogonial stem cell (SSC) niche, where homeostasis is maintained by the interplay of several signaling pathways and growth factors. These factors are secreted by Sertoli cells located within the seminiferous epithelium, and by interstitial cells residing between the seminiferous tubules. Sertoli cells and peritubular myoid cells produce glial cell line-derived neurotrophic factor (GDNF), which binds to the RET/GFRA1 receptor complex at the surface of undifferentiated spermatogonia. Read More

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http://dx.doi.org/10.1530/REP-18-0239DOI Listing
January 2019
1 Read

Ubiquitin-proteasome system participates in the de-aggregation of spermadhesin and DQH protein during boar sperm capacitation.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

P Sutovsky, S141 ASRC, University of Missouri-Columbia, Columbia, 65211-5300, United States.

We studied the participation of the ubiquitin proteasome system (UPS) in spermadhesin release during in vitro capacitation (IVC) of domestic boar spermatozoa. At ejaculation, boar spermatozoa acquire low molecular weight (8-16 kDa) seminal plasma proteins, predominantly spermadhesins, aggregated on the sperm surface. Due to their arrangement, such aggregates are relatively inaccessible to antibody labeling. Read More

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http://dx.doi.org/10.1530/REP-18-0413DOI Listing
January 2019
1 Read

CXCL16/CXCR6 interaction Promotes Endometrial Decidualization via the PI3K/ AKT Pathway.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

H Sun, nanjing drum tower hospital, Nanjing Univ, nanjing, China.

Decidualization renders the endometrium transiently receptive to an implanting blastocyst although the underlying mechanisms remain incompletely understood. The aim of this study was to determine the role of chemokine CXCL16 and its receptor CXCR6 in the decidualization during pregnancy. Here, the expression of CXCL16 was investigated in endometrial tissues, decidua, and placenta in this study. Read More

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http://dx.doi.org/10.1530/REP-18-0417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365678PMC
January 2019
1 Read

Cytotoxic effects of Shiga toxin-2 on human extravillous trophoblast cell lines.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

F Sacerdoti, Departamento de Ciencias Fisiológicas, Instituto de Fisiología y Biofísica (IFIBIO) Houssay, Facultad de Medicina, Universidad de Buenos Aires, CABA, Argentina.

Shiga toxin (Stx2) producing Escherichia coli infections during early gestation may impair placentation through a Stx2 damage of extravillous trophoblast (EVT) cells. We have previously demonstrated that Stx2 injected in rats in the early stage of pregnancy causes spontaneous abortion by a direct cytotoxic effect in the highly perfused feto-uteroplacental unit. The main aim was to evaluate the effects of Stx2 on EVT in order to understand the possible adverse effects that the toxin may cause on trophoblast cells during early pregnancy. Read More

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http://dx.doi.org/10.1530/REP-18-0581DOI Listing
January 2019
2 Reads

Cervical mucus sialic acid content determines the progression of thawed ram sperm through the cervix.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

S Fair, Life Sciences, University of Limerick, limerick, Ireland.

The aim of this study was to investigate the properties and to functionally characterise the cervical mucus that modulates sperm transport through the cervix by using ewe breeds with a divergent pregnancy rate (Belclare and Suffolk; high and low, respectively) following cervical insemination using frozen-thawed semen. Sperm number, as well as sialic acid and fucose content in both the channels and in the lumen of different regions of the cervix were quantified in inseminated Belclare and Suffolk ewes. Expression of glycosyltransferase and MUC genes, glycosidase activity and sialic acid speciation in follicular phase cervical tissue and mucus were assessed. Read More

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http://dx.doi.org/10.1530/REP-18-0547DOI Listing
January 2019
2 Reads

Emerging Role for SRC family kinases in junction dynamics during spermatogenesis.

Reproduction 2019 Jan 1. Epub 2019 Jan 1.

Y Ni, Department of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, China.

SRC family kinases (SFKs) are known regulators of multiple cellular events, including cell movement, differentiation, proliferation, survival, and apoptosis. SFKs are expressed virtually by all mammalian cells. They are non-receptor protein kinases that phosphorylate a variety of cellular proteins on tyrosine, leading to activation of protein targets in response to environmental stimuli. Read More

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https://rep.bioscientifica.com/view/journals/rep/aop/rep-18-
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http://dx.doi.org/10.1530/REP-18-0440DOI Listing
January 2019
6 Reads

Trophoblast-derived hyaluronan promotes the regulatory phenotype of decidual macrophages.

Reproduction 2019 Feb;157(2):189-198

The Affiliated Suzhou Hospital of Nanjing Medical University/Suzhou Municipal Hospital, Suzhou, China.

There is delicate crosstalk between fetus-derived trophoblasts (Tros) and maternal cells during normal pregnancy. Dysfunctions in interaction are highly linked to some pregnancy complications, such as recurrent spontaneous abortion (RSA), pre-eclampsia and fetal growth restriction. Hyaluronan (HA), the most abundant component of extracellular matrix, has been reported to act as both a pro- and an anti-inflammatory molecule. Read More

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https://rep.bioscientifica.com/view/journals/rep/157/2/REP-1
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http://dx.doi.org/10.1530/REP-18-0450DOI Listing
February 2019
10 Reads

In vitro and in vivo mouse follicle development in ovaries and reaggregated ovaries.

Reproduction 2019 Feb;157(2):135-148

Nuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, Level 3, John Radcliffe Hospital, Oxford, United Kingdom.

Follicle development requires complex and coordinated interactions between both the oocyte and its associated somatic cells. In ovarian dysfunction, follicle development may be abnormal due to defective somatic cell function; for example, premature ovarian insufficiency or malignancies. Replacing defective somatic cells, using the reaggregated ovary (RO) technique, may 'rescue' follicle development. Read More

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http://dx.doi.org/10.1530/REP-18-0115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347279PMC
February 2019
3 Reads

Acute restraint stress triggers progesterone withdrawal and endometrial breakdown and shedding through corticosterone stimulation in mouse menstrual-like model.

Reproduction 2019 Feb;157(2):149-161

Reproductive Physiology Laboratory, National Research Institute for Family Planning, Beijing, China.

Stress impacts the reproductive axis at the level of the hypothalamus and the pituitary gland, which exert an effect on the ovary. Menstruation is regulated by the hypothalamic-pituitary-ovary (HPO) axis. However, the role of stress in menstruation remains unclear. Read More

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https://rep.bioscientifica.com/view/journals/rep/157/2/REP-1
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http://dx.doi.org/10.1530/REP-18-0163DOI Listing
February 2019
7 Reads

Stearoyl-coenzyme A desaturase 1 is required for lipid droplet formation in pig embryo.

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

C Lee, Food and Animal Biotechnology, Seoul National University, Seoul, Korea (the Republic of).

Lipid droplets (LD) provide a source of energy, and their importance during embryogenesis has been increasingly recognized. In particular, pig embryos have larger amounts of intercellular lipid bilayers than other mammalian species, suggesting that porcine embryos are more dependent on lipid metabolic pathways. The objective of the present study was to detect the effect of stearoyl-coenzyme A desaturase 1 (SCD1) on LD formation and to associate these effects with the mRNA abundance of LD formation-related genes (SREBP, ARF1, COPG2, PLD1, and ERK2) in in vitro-produced porcine embryos. Read More

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http://dx.doi.org/10.1530/REP-18-0556DOI Listing
December 2018
1 Read

Blastocysts depict sex-specific signalling of IFNT transcription, translation and activity.

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

S Ulbrich, Animal Physiology, Eidgenossische Technische Hochschule Zurich Institut fur Agrarwissenschaften, Zurich, Switzerland.

Preimplantation bovine blastocyst supernatants exhibit sex-dependent antiviral activity, due to the ruminant pregnancy recognition signal Interferon tau (IFNT). Differing potencies of IFNT variants have been supposed as cause, although evidence remains scarce. Here, we aimed at quantifying the sex-dependent IFNT production on transcriptional, translational, and biological activity level in bovine blastocysts, to elucidate the origin of differences in antiviral activity between male and female blastocysts. Read More

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http://dx.doi.org/10.1530/REP-18-0312DOI Listing
December 2018
1 Read

The Cytoskeleton in Spermatogenesis.

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

L O'Donnell, Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Australia.

As germ cells progress through spermatogenesis, they undergo a dramatic transformation, wherein a single, diploid spermatogonial stem cell ultimately produces thousands of highly specialised, haploid spermatozoa. The cytoskeleton is an integral aspect of all eukaryotic cells. It concomitantly provides both structural support and functional pliability, performing key roles in many fundamental processes including, motility, intracellular trafficking, differentiation and cell division. Read More

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http://dx.doi.org/10.1530/REP-18-0457DOI Listing
December 2018
10 Reads

Med20 is essential for early embryogenesis and regulates Nanog expression.

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

J Mager, Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, United States.

Mediator is an evolutionarily conserved multi-subunit complex, bridging transcriptional activators and repressors to the general RNA polymerase II (Pol II) initiation machinery. Though the Mediator complex is crucial for transcription of almost all Pol II promoters in eukaryote organisms, the phenotypes of individual Mediator subunit mutants are each distinct. Here we report for the first time, the essential role of subunit Med20 in early mammalian embryo development. Read More

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http://dx.doi.org/10.1530/REP-18-0508DOI Listing
December 2018
1 Read

Thrombospondin-1 at the crossroads of corpus luteum fate decisions.

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

R Meidan, Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.

The multimodular matricellular protein thrombospondin-1 (THBS1) was among the first identified endogenous antiangiogenic molecules. Recent studies have shown THBS1-mediated suppression of angiogenesis and other critical activities for corpus luteum (CL) regression. THBS1 is specifically induced by prostaglandin F2alpha in mature CL undergoing regression, whereas luteinizing signals such as luteinizing hormone and insulin reduced its expression. Read More

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http://dx.doi.org/10.1530/REP-18-0530DOI Listing
December 2018
1 Read

My Womban's Life: Understanding Human Endometrial Function.

Authors:
Lois Salamonsen

Reproduction 2018 Dec 1. Epub 2018 Dec 1.

L Salamonsen, Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Australia.

The focus of my life in science, has been to improve reproductive health for women, with an emphasis on the endometrium, the most dynamic tissue in the human body: its remarkable cyclical remodelling is essential for establishment of pregnancy. The most notable events in a woman's endometrial cycle are menstruation and endometrial repair, regeneration of the endometrium during the proliferative phase, attainment of receptivity by the mid-secretory phase of the cycle and the embryo-maternal interactions that initiate peri-implantation events within the microenvironment of the uterine cavity. I have contributed to understanding the molecular and cellular changes underpinning these events, and how disturbance of them leads to menstrual disorders, infertility and endometrial diseases including abnormal uterine bleeding, endometriosis and endometrial cancer. Read More

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http://dx.doi.org/10.1530/REP-18-0518DOI Listing
December 2018
2 Reads

Estradiol promotes EMT in endometriosis via MALAT1/miR200s sponge function.

Reproduction 2018 Nov 1. Epub 2018 Nov 1.

L Zhang, Department of Obstetrics and Gynecology, Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Endometriosis is an estrogen-dependent benign gynecological disease that shares some common features of malignancy. Epithelial-mesenchymal transition (EMT) has been recognized as a core mechanism of endometriosis. MALAT1 is widely known as EMT promoter, while miR200 family members (miR200s) are considered as EMT inhibitors. Read More

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http://dx.doi.org/10.1530/REP-18-0424DOI Listing
November 2018
3 Reads
3.174 Impact Factor

It Takes Two (Centrioles) to Tango.

Reproduction 2018 Nov 1. Epub 2018 Nov 1.

E Fishman, Biological Sciences, University of Toledo, Toledo, United States.

Cells that divide during embryo development require precisely two centrioles during interphase and four centrioles during mitosis. This precise number is maintained by allowing each centriole to nucleate only one centriole per cell cycle (i.e. Read More

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http://dx.doi.org/10.1530/REP-18-0350DOI Listing
November 2018
23 Reads

Inhibition of RNA synthesis during Scriptaid exposure enhances gene reprogramming in SCNT embryos.

Reproduction 2018 Nov 1. Epub 2018 Nov 1.

V Bordignon, Department of Animal Sciences, McGill University, Ste-Anne-de-Bellevue, H9X3V9, Canada.

Insufficient epigenetic reprogramming is incompatible with normal development of embryos produced by somatic cell nuclear transfer (SCNT), but treatment with histone deacetylases inhibitors (HDACi) enhance development of SCNT embryos. However, the mechanisms underpinning HDACi benefits in SCNT embryos remain largely uncharacterized. We hypothesized that, in addition to enhancing reprogramming, HDACi treatment may promote expression of genes not required for early development of SCNT embryos. Read More

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http://dx.doi.org/10.1530/REP-18-0366DOI Listing
November 2018
12 Reads

Different pre-implantation phenotypes of bovine blastocysts produced in vitro.

Reproduction 2018 Nov 1. Epub 2018 Nov 1.

M Sirard, Faculté des sciences de l'agriculture et de l'alimentation, Université Laval, Centre de Recherche en Biologie de la Reproduction, Département des Sciences Animales, Quebec City, Canada.

Embryo transfer in cattle is performed with blastocysts produced in vivo or in vitro using defined media. However, outdated systems such as those that use serum and co-culture remain of interest for research purposes. Here, we investigated the effect of additional culture time on in vitro-produced embryos. Read More

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http://dx.doi.org/10.1530/REP-18-0439DOI Listing
November 2018
7 Reads

Slo3 K+ channel blocker clofilium extends bull and mouse sperm-fertilizing competence.

Reproduction 2018 Dec;156(6):463-476

Université Grenoble Alpes, Grenoble, France.

For artificial insemination (AI) to be successful, it is essential that sperm delivery be perfectly timed relative to ovulation, as sperm lifespan is limited due to oxidative metabolism induced by capacitation. Extending the window of sperm capacitation could therefore increase sperm lifespan, prolong sperm-fertilizing competence and increase AI efficiency. Hyperpolarization of sperm is a crucial step in capacitation and is induced by activation of the potassium calcium-activated channel subfamily U member 1 (KCNU1, also named Slo3 or KSper). Read More

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https://rep.bioscientifica.com/view/journals/rep/156/6/REP-1
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http://dx.doi.org/10.1530/REP-18-0075DOI Listing
December 2018
14 Reads
3.174 Impact Factor

Identification and characterization of ERV transcripts in goat embryos.

Reproduction 2018 Nov 1. Epub 2018 Nov 1.

J Liu, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest Agriculture and Forestry University, Yangling, China.

Endogenous retroviruses (ERVs), which are abundant in mammalian genomes, can modulate the expression of nearby genes, and their expression is dynamic and stage specific during early embryonic development in mice and humans. However, the functions and mechanisms of ERV elements in regulating embryonic development remain unclear. Here, we utilized several methods to determine the contribution of ERVs to the makeup and regulation of transcripts during embryonic genome activation (EGA). Read More

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http://dx.doi.org/10.1530/REP-18-0336DOI Listing
November 2018
11 Reads
3.174 Impact Factor

Decidual expression and regulation of fatty acid desaturase 3 during mouse decidualization.

Reproduction 2018 Nov;156(5):429-437

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Decidualization is required for the successful establishment of pregnancy in rodents and primates. Fatty acid desaturase 3 (Fads3) belongs to the fatty acid desaturase family, which is a crucial enzyme for highly unsaturated fatty acid biosynthesis. However, the expression, regulation and function of Fads3 during early pregnancy in mice are still unknown. Read More

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http://dx.doi.org/10.1530/REP-18-0145DOI Listing
November 2018
1 Read

Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

Y Sato, Obstetrics and Gynecology, Otsu Red Cross Hospital, Otsu, Japan.

The endometrium extracellular matrix (ECM) is essential for embryo implantation. Versican, a large chondroitin sulfate proteoglycan that binds hyaluronan and forms large ECM aggregates, can influence fundamental physiological phenomena, such as cell proliferation, adhesion, and migration. The present study investigated the possible role of versican in human embryo implantation. Read More

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http://dx.doi.org/10.1530/REP-18-0333DOI Listing
October 2018
8 Reads

Sertoli-immature spermatids disengagement during testis regression in the armadillo.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

S Garagna, Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, Pavia, Italy.

In Nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining spermatocytes. At the end of the regression period, only spermatogonia and Sertoli cells persist in the seminiferous epithelium. Read More

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http://dx.doi.org/10.1530/REP-18-0006DOI Listing
October 2018
8 Reads

Modification of sperm fatty acid composition during epididymal maturation in bats.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

P Christe, Department of Ecology and Evolution, Universite de Lausanne Departement d'ecologie et evolution, Lausanne, Switzerland.

Biochemical properties of polyunsaturated fatty acids (PUFAs) are fundamental to sperm movements. Amongst all adjustments operated during epididymal maturation, sperm membrane lipid composition is remodelled. Specifically, the proportion of PUFAs usually increases from the caput towards the cauda epididymidis. Read More

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http://dx.doi.org/10.1530/REP-18-0463DOI Listing
October 2018
10 Reads

How does chemotherapy treatment damage the prepubertal testis?

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

N Spears, Biomedical Sciences, University of Edinburgh, Edinburgh, United Kingdom of Great Britain and Northern Ireland.

Chemotherapy treatment is a mainstay of anticancer regimens, significantly contributing to the recent increase in childhood cancer survival rates. Conventional cancer therapy not only targets malignant but also healthy cells resulting in side effects including infertility. For prepubertal boys, there are currently no fertility preservation strategies in use, although several potential methods are under investigation. Read More

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http://dx.doi.org/10.1530/REP-18-0221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347281PMC
October 2018
1 Read

Gene expression in sheep fetal ovaries exposed to gestational under nutrition.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

J Stanton, Department of Anatomy, University of Otago, Dunedin, New Zealand.

A number of studies have demonstrated effects of gestational undernutrition on fetal ovarian development and postnatal female fertility. However the mechanism underlying these effects remains elusive. Using a cohort of animals in which altered gestational nutrition affected indicators of postnatal fertility, this study applies RNAseq to fetal ovaries to identify affected genes and pathways that may underlie the relationship between gestational plane of nutrition and postnatal fertility. Read More

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http://dx.doi.org/10.1530/REP-18-0358DOI Listing
October 2018
11 Reads

Follicle rupture during ovulation with an emphasis on recent progress in fish models.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

K Ogiwara, Reproductive and Developmental Biology, Hokkaido University Faculty of Science Graduate School of Science, Sapporo, Japan.

Ovulation, which is induced by the ovulatory luteinizing hormone (LH) surge, is a dynamic process that results in a discharge of one or more fertilizable oocytes from the ovarian follicle into the ovarian cavity or into the abdominal cavity. Follicle rupture is a core event of the ovulatory process and has been the subject of intensive investigation. Many studies have been performed in various vertebrate animals that focused on proteolysis during ovulation. Read More

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http://dx.doi.org/10.1530/REP-18-0251DOI Listing
October 2018
9 Reads

Endometrial mesenchymal stem/stromal cell modulation of T cell proliferation.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

J Deane, The Ritchie Centre, Hudson Institute of Medical Research, Clayton, Australia.

Perivascular mesenchymal stem/stromal cells can be isolated from the human endometrium using the surface marker SUSD2, and are being investigated for use in tissue repair. Mesenchymal stem/stromal cells from other tissues modulate T cell responses via mechanisms including interleukin-10, prostaglandin E2, TGF-β1 and regulatory T cells. Animal studies demonstrate that endometrial mesenchymal stem/stromal cells can also modify immune responses to implanted mesh, but the mechanism/s they employ have not been explored. Read More

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http://dx.doi.org/10.1530/REP-18-0266DOI Listing
October 2018
25 Reads

Effects of intravenous infusion of E.coli lipopolysaccharide in early pregnant cows.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

H Bollwein, Clinic of Reproductive Medicine, University of Zurich, Zurich, 8057, Switzerland.

The objective was to characterize effects of Escherichia coli LPS (given iv) on corpus luteum (CL) and embryonic viability in early pregnant cattle. Eight non-lactating German Holstein cows were given 0.5 µg/kg LPS on 35 ± 3 d (mean ± SEM) of pregnancy, whereas seven heifers, 41 ± 6 d pregnant, were given 10 ml saline (Control group). Read More

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http://dx.doi.org/10.1530/REP-18-0174DOI Listing
October 2018
14 Reads

Effects of androgen and oestrogen on the IGF pathways controlling phallus growth.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

M Renfree, School of BioSciences, The University of Melbourne, Melbourne, Australia.

The development of the mammalian phallus involves hormone-dependent mesenchymal-epithelial signalling mechanisms that contribute to urethral closure and regulation of phallus elongation and growth. In marsupials, most differentiation and growth of the phallus occurs post-natally, making them amenable to direct hormone treatment. Expression of IGFs, FGFs, EFNB2, MAFB, DLX5 and AP-1 mRNAs in the phallus at day 50 post-partum (pp) were altered after treatment of tammar wallaby young from day 20-40 pp with androgen, oestrogen or after castration at day 25pp. Read More

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http://dx.doi.org/10.1530/REP-18-0416DOI Listing
October 2018
2 Reads

Driving gonadotrophin hormone receptor signalling; the role of membrane trafficking.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

A Hanyaloglu, Institute of Reproductive and Developmental Biology, Imperial College London, London, United Kingdom of Great Britain and Northern Ireland.

Our understanding of G protein-coupled receptor (GPCR) signalling has significantly evolved over the past decade, whereby signalling not only occurs from the plasma membrane but continues, or is reactivated, following internalisation in to endosomal compartments. The spatial organisation of GPCRs is thus essential to decode dynamic and complex signals, and to activate specific downstream pathways that elicit the appropriate cellular response. For the gonadotrophin hormone receptors, membrane trafficking has been demonstrated to play a significant role in regulating its signal activity that in turn would impact at physiological and even pathophysiological level. Read More

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http://dx.doi.org/10.1530/REP-18-0423DOI Listing
October 2018
3 Reads

Connexin hemichannels and cell death as measures of bovine COC vitrification success.

Reproduction 2018 Oct 1. Epub 2018 Oct 1.

L Leybaert, Physiology group, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Universiteit Gent, Gent, Belgium.

Vitrification of immature germinal vesicle-stage oocytes is a promising method in assisted reproduction but is associated with reduced developmental potential and low birth rates. Cumulus-oocyte complexes (COCs) express several connexins that form hexameric hemichannels, which interact head-to-head to create a gap junction or exist as unopposed free hemichannels. The latter are normally closed but open under stress conditions and may exert detrimental effects. Read More

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http://dx.doi.org/10.1530/REP-18-0387DOI Listing
October 2018
1 Read