1,756 results match your criteria Reoviruses


Real-Time Dissecting the Entry and Intracellular Dynamics of Single Reovirus Particle.

Front Microbiol 2018 20;9:2797. Epub 2018 Nov 20.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Reoviruses are non-enveloped viruses with wide host range, can cause serious infections in animals, plants and microorganism, e.g., aquareovirus, which is capable of causing serious haemorrhagic in aquatic animals. Read More

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November 2018
1 Read

Isolation and genomic characterization of a novel chicken-orign orthoreovirus causing goslings hepatitis.

Vet Microbiol 2018 Dec 25;227:69-77. Epub 2018 Oct 25.

College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai'an, Shandong Province, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, 271018, China. Electronic address:

A severe infectious disease characterized by nephritis, hepatitis and splenitis has attacked goslings around Shandong province in China since 2016. A novel chicken-origin avian orthoreovirus (ARV) was isolated with LMH cells from affected goslings named Reo/Goose/SDPY/1116/17 (SDPY-ARV) strain, and the infection was successfully reproduced experimentally. The ARV-SDPY full genome sequencing was conducted using Next-Generation Sequencing (NGS) technique on Illumina HiSeq platform. Read More

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December 2018
1 Read

Arming oncolytic reovirus with GM-CSF gene to enhance immunity.

Cancer Gene Ther 2018 Nov 23. Epub 2018 Nov 23.

Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands.

Oncolytic reovirus administration has been well tolerated by cancer patients in clinical trials. However, its anti-cancer efficacy as a monotherapy remains to be augmented. We and others have previously demonstrated the feasibility of producing replication-competent reoviruses expressing a heterologous transgene. Read More

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November 2018
4 Reads

Oncolytic viruses and checkpoint inhibitors: combination therapy in clinical trials.

Clin Transl Med 2018 Nov 14;7(1):35. Epub 2018 Nov 14.

Division of Surgical Oncology, Department of Surgery, City of Hope National Medical Center, 1500 E Duarte Road, Duarte, CA, 91010, USA.

Advances in the understanding of cancer immunotherapy and the development of multiple checkpoint inhibitors have dramatically changed the current landscape of cancer treatment. Recent large-scale phase III trials (e.g. Read More

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November 2018
4 Reads

Serological evidence of human infection by bat orthoreovirus in Singapore.

J Med Virol 2018 Nov 8. Epub 2018 Nov 8.

Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays. Our findings confirm that PRV spillover into human populations is relatively common in this region of the world. Read More

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November 2018
5 Reads

DNA vaccine expressing the non-structural proteins of Piscine orthoreovirus delay the kinetics of PRV infection and induces moderate protection against heart -and skeletal muscle inflammation in Atlantic salmon (Salmo salar).

Vaccine 2018 Nov 2;36(50):7599-7608. Epub 2018 Nov 2.

Department of Food Safety and Infectious Biology, Norwegian University of Life Sciences, 0454 Oslo, Norway. Electronic address:

Piscine orthoreovirus (PRV) causes heart- and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). Erythrocytes are the main target cells for PRV. HSMI causes significant economic losses to the salmon aquaculture industry, and there is currently no vaccine available. Read More

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November 2018
4 Reads

Components of the reovirus capsid differentially contribute to stability.

J Virol 2018 Oct 31. Epub 2018 Oct 31.

Department of Biology, Indiana University, Bloomington, Indiana, USA

The mammalian orthoreovirus (reovirus) outer capsid is composed of 200 μ1-σ3 heterohexamers and a maximum of 12 σ1 trimers. During cell entry, σ3 is degraded by luminal or intracellular proteases to generate the infectious subviral particle (ISVP). When ISVP formation is prevented, reovirus fails to establish a productive infection, suggesting proteolytic priming is required for entry. Read More

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October 2018
5 Reads

Impact of Two Reoviruses and Their Coinfection on the Rice RNAi System and vsiRNA Production.

Viruses 2018 Oct 30;10(11). Epub 2018 Oct 30.

Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.

Both (SRBSDV) and (RRSV) belong to the family , and synergistic infection of these two viruses commonly occurs in the field. This study revealed that both SRBSDV and RRSV affect the RNA interference (RNAi) pathway and form different virus-derived interfering RNA (vsiRNA) profiles in rice. Co-infection of rice by SRBSDV and RRSV up-regulated the expression of rice DICER-like (DCL) proteins but down-regulated the expression of rice RNA-dependent RNA polymerases (RDRs), and the accumulation of vsiRNAs of either RBSDV or RRSV was decreased compared with that in singly infected plants. Read More

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October 2018
1 Read

Identification of a piscine reovirus-related pathogen in proliferative darkening syndrome (PDS) infected brown trout (Salmo trutta fario) using a next-generation technology detection pipeline.

PLoS One 2018 22;13(10):e0206164. Epub 2018 Oct 22.

Aquatic Systems Biology Unit, Department of Ecology and Ecosystem Management, Technical University of Munich, Freising, Germany.

The proliferative darkening syndrome (PDS) is an annually recurring disease that causes species-specific die-off of brown trout (Salmo trutta fario) with a mortality rate of near 100% in pre-alpine rivers of central Europe. So far the etiology and causation of this disease is still unclear. The objective of this study was to identify the cause of PDS using a next-generation technology detection pipeline. Read More

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October 2018

Comparative proteomic analysis revealed complex responses to classical/novel duck reovirus infections in the spleen tissue of Cairna moschata.

J Proteomics 2018 Oct 16. Epub 2018 Oct 16.

Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address:

Duck reovirus (DRV), a member of the genus Orthoreovirus in the family Reoviridae, was first isolated from Muscovy ducks. The disease associated with DRV causes great economic losses to the duck industry. However, the responses of duck (Cairna moschata) to the classical/novel DRV (C/NDRV) infections are largely unknown. Read More

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October 2018
6 Reads

Mass spectrometry-based identification and whole-genome characterisation of the first pteropine orthoreovirus isolated from monkey faeces in Thailand.

BMC Microbiol 2018 Oct 17;18(1):135. Epub 2018 Oct 17.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.

Background: The pteropine orthoreovirus (PRV) was isolated from monkey (Macaca fascicularis) faecal samples collected from human-inhabited areas in Lopburi Province, Thailand. These samples were initially obtained to survey for the presence of hepatitis E virus (HEV).

Results: Two virus isolates were retrieved by virus culture of 55 monkey faecal samples. Read More

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October 2018
1 Read

Evaluation of the pathogenicity of mammalian orthoreovirus type 3 (MRV3) in germ-free gnotobiotic pigs and of the efficacy of an inactivated vaccine against MRV3 infection in neonatal conventional piglets.

Vet Microbiol 2018 Oct 22;224:23-30. Epub 2018 Aug 22.

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States. Electronic address:

A novel U.S. strain of mammalian orthoreovirus type 3 (MRV3) isolated from diarrheic pigs in 2015 was reportedly highly pathogenic in pigs. Read More

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October 2018
5 Reads

Isolation of Pteropine orthoreovirus from Pteropus vampyrus in Garut, Indonesia.

Virus Genes 2018 Dec 19;54(6):823-827. Epub 2018 Sep 19.

Laboratory of Animal Morphology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.

Flying foxes belonging to the genus Pteropus are known to be reservoirs of zoonotic viruses. In this study, we describe the isolation of Pteropine orthoreovirus (PRV) from rectal swab samples of Pteropus vampyrus in Indonesia. PRV is an emerging zoonotic respiratory virus that can be transmitted from bats to humans. Read More

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December 2018
6 Reads

Complete genome sequence of a novel avian orthoreovirus isolated from gosling, China.

Arch Virol 2018 Sep 12. Epub 2018 Sep 12.

College of Animal Science and Veterinary Medicine, Shandong Agriculture University, No. 61 Daizong Street, Tai'an, 271018, China.

Avian orthoreovirus (ARV) has been considered as a significant pathogen causing great infectious diseases to the avian, like broiler and waterfowl. The genome of this novel ARV(Reo/SDPY/Goose) was completely sequenced by next-generation sequencing. The complete genome was found to be 23517 bp in length with 10 segments. Read More

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September 2018
5 Reads

Reovirus Neurotropism and Virulence Are Dictated by Sequences in the Head Domain of the Viral Attachment Protein.

J Virol 2018 Dec 12;92(23). Epub 2018 Nov 12.

Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Viral capsid components that bind cellular receptors mediate critical functions in viral tropism and disease pathogenesis. Mammalian orthoreoviruses (reoviruses) spread systemically in newborn mice to cause serotype-specific disease in the central nervous system (CNS). Serotype 1 (T1) reovirus infects ependymal cells to cause nonlethal hydrocephalus, whereas serotype 3 (T3) reovirus infects neurons to cause fulminant and lethal encephalitis. Read More

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December 2018

Capsid Structure of dsRNA Fungal Viruses.

Viruses 2018 09 7;10(9). Epub 2018 Sep 7.

Department of Structure of Macromolecules, Centro Nacional de Biotecnología (CNB-CSIC), Campus Cantoblanco, 28049 Madrid, Spain.

Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. Read More

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September 2018

Genomic sequence and phylogenetic analyses of two novel orthoreovirus strains isolated from Pekin ducks in 2014 in Germany.

Virus Res 2018 09 5;257:57-62. Epub 2018 Sep 5.

Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.

Complete genomic sequences of two orthoreovirus strains, D2533/4/1-10 and D2533/6/1-10, isolated from Pekin ducklings in Germany have been determined. Pairwise sequence comparisons and phylogenetic analyses indicated that strain D2533/4/1-10 might have acquired its genomic segments from three different origins, from classical and novel waterfowl reoviruses, and a yet unknown orthoreovirus strain. D2533/6/1-10 proved to be only distantly related to previously described orthoreoviruses. Read More

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September 2018
10 Reads

Identification of novel reassortant mammalian orthoreoviruses from bats in Slovenia.

BMC Vet Res 2018 Sep 3;14(1):264. Epub 2018 Sep 3.

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška cesta 4, SI-1000, Ljubljana, Slovenia.

Background: Recently, mammalian orthoreoviruses (MRVs) were detected for the first time in European bats, and the closely related strain SI-MRV01 was isolated from a child with severe diarrhoea in Slovenia. Genetically similar strains have also been reported from other mammals, which reveals their wide host distribution. The aim of this study was to retrospectively investigate the occurrence and genetic diversity of MRVs in bats in Slovenia, from samples obtained throughout the country in 2008 to 2010, and in 2012 and to investigate the occurrence of the novel SI-MRV01 MRV variant in Slovenian bats. Read More

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September 2018
12 Reads

Reovirus σNS and μNS Proteins Remodel the Endoplasmic Reticulum to Build Replication Neo-Organelles.

MBio 2018 Aug 7;9(4). Epub 2018 Aug 7.

Cell Structure Laboratory, National Center for Biotechnology, CNB-CSIC, Campus UAM, Madrid, Spain

Like most viruses that replicate in the cytoplasm, mammalian reoviruses assemble membranous neo-organelles called inclusions that serve as sites of viral genome replication and particle morphogenesis. Viral inclusion formation is essential for viral infection, but how these organelles form is not well understood. We investigated the biogenesis of reovirus inclusions. Read More

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August 2018
1 Read

The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses.

Curr Opin Virol 2018 Aug 3;33:55-65. Epub 2018 Aug 3.

Institute of Plant Science and Resources (IPSR), Okayama University, Chuou 2-20-1, Kurashiki Okayama 710-0046, Japan. Electronic address:

Many fungal viruses or mycoviruses have multi-segmented, rather than single-segmented, genomes. This multi-segment nature is frequently possessed by double-stranded RNA viruses, which include members of the Chrysoviridae, Quadriviridae, Megabirnaviridae, Partitiviridae, and Reoviridae families, and unassigned groups. Their genome segments are often packaged separately with the exception of mycoreoviruses, which are multi-segmented but mono-particulate viruses. Read More

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August 2018
1 Read

Structures of the Polymerase Complex and RNA Genome Show How Aquareovirus Transcription Machineries Respond to Uncoating.

J Virol 2018 Nov 12;92(21). Epub 2018 Oct 12.

Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA

Reoviruses carry out genomic RNA transcription within intact viruses to synthesize plus-sense RNA strands, which are capped prior to their release as mRNA. The structures of the transcriptional enzyme complex (TEC) containing the RNA-dependent RNA polymerase (RdRp) and NTPase are known for the single-layered reovirus cytoplasmic polyhedrosis virus (CPV), but not for multilayered reoviruses, such as aquareoviruses (ARV), which possess a primed stage that CPV lacks. Consequently, how the RNA genome and TEC respond to priming in reoviruses is unknown. Read More

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November 2018
1 Read

Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1.

Virol J 2018 07 27;15(1):114. Epub 2018 Jul 27.

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Leipzig, Germany.

Background: Reovirus type-3 infections cause severe pathologies in young mice and thus influence animal experiments in many ways. Therefore, the Federation of Laboratory Animal Science Associations (FELASA) recommends an annual screening in laboratory mice as part of a thorough health monitoring program. Based on the high protein sequence homology among the different reovirus serotypes, immunofluorescence antibody assay and other indirect methods relying on the whole virus are presumably cross-reactive to antibodies triggered by mammalian orthoreovirus infections independent of the serotype. Read More

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July 2018
9 Reads

Molecular characterization of emerging avian reovirus variants isolated from viral arthritis cases in Western Canada 2012-2017 based on partial sigma (σ)C gene.

Virology 2018 09 18;522:138-146. Epub 2018 Jul 18.

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 2C53, 3330 Hospital Drive NW, Calgary, AB, Canada T2N 4N1. Electronic address:

Viral Arthritis (VA), a disease caused by Avian Reovirus (ARV), has emerged as a significant cause of economic losses in broiler chicken flocks in Western Canada. These outbreaks were characterized by 4-13% morbidity, followed by a spike in mortality/culling that in extreme cases required total flock depopulation. From 2012-2017, 38 ARV isolates were recovered. Read More

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September 2018
8 Reads

Characterization of a replicating expanded tropism oncolytic reovirus carrying the adenovirus E4orf4 gene.

Gene Ther 2018 08 16;25(5):331-344. Epub 2018 Jul 16.

Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands.

While the mammalian orthoreovirus type 3 dearing (reovirus T3D) infects many different tumour cells, various cell lines resist the induction of reovirus-mediated cell death. In an effort to increase the oncolytic potency, we introduced transgenes into the S1 segment of reovirus T3D. The adenovirus E4orf4 gene was selected as transgene since the encoded E4orf4 protein induces cell death in transformed cells. Read More

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August 2018
1 Read

Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil.

Poult Sci 2018 Oct;97(10):3550-3555

Faculdade de Medicina Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul 95320-000, Brasil.

Avian reoviruses (ARVs) can infect a variety of species worldwide. Birds can present stunting syndrome, respiratory and/or enteric diseases, immunosuppression, malabsorption, viral arthritis/tenosynovitis, and even secondary infections by other microorganisms. Flaws in conventional vaccines and the increase in the diagnostic rate of disease in the last 5 yr suggest the emergence of pathogenic ARVs in the poultry flocks worldwide. Read More

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October 2018
22 Reads

Detection of Avian Reoviruses in Wild Birds in Poland.

J Vet Res 2017 Sep 19;61(3):239-245. Epub 2017 Sep 19.

Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland.

Introduction: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos.

Material And Methods: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. Read More

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September 2017
1 Read

Comparative proteomic analysis revealed complex responses to classical/novel duck reovirus infections in Cairna moschata.

Sci Rep 2018 Jul 4;8(1):10079. Epub 2018 Jul 4.

Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Duck reovirus (DRV) is an typical aquatic bird pathogen belonging to the Orthoreovirus genus of the Reoviridae family. Reovirus causes huge economic losses to the duck industry. Although DRV has been identified and isolated long ago, the responses of Cairna moschata to classical/novel duck reovirus (CDRV/NDRV) infections are largely unknown. Read More

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July 2018
4 Reads

Current understanding of reovirus oncolysis mechanisms.

Oncolytic Virother 2018 14;7:53-63. Epub 2018 Jun 14.

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Atlanta, GA, USA.

Mammalian orthoreovirus (reovirus) is under development as a cancer virotherapy. Clinical trials demonstrate that reovirus-based therapies are safe and tolerated in patients with a wide variety of cancers. Although reovirus monotherapy has proven largely ineffective, reovirus sensitizes cancer cells to existing chemotherapeutic agents and radiation. Read More

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June 2018
1 Read

Structure of RNA polymerase complex and genome within a dsRNA virus provides insights into the mechanisms of transcription and assembly.

Proc Natl Acad Sci U S A 2018 07 25;115(28):7344-7349. Epub 2018 Jun 25.

School of Life Sciences, Tsinghua University, Beijing 100084, China;

Most double-stranded RNA (dsRNA) viruses transcribe RNA plus strands within a common innermost capsid shell. This process requires coordinated efforts by RNA-dependent RNA polymerase (RdRp) together with other capsid proteins and genomic RNA. Here we report the near-atomic resolution structure of the RdRp protein VP2 in complex with its cofactor protein VP4 and genomic RNA within an aquareovirus capsid using 200-kV cryoelectron microscopy and symmetry-mismatch reconstruction. Read More

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July 2018
13 Reads

Identification and characterization of novel mosquito-borne (Kammavanpettai virus) and tick-borne (Wad Medani) reoviruses isolated in India.

J Gen Virol 2018 Aug 25;99(8):991-1000. Epub 2018 Jun 25.

1​Maximum Containment Laboratory, National Institute of Virology, Pune, Maharashtra, India.

In 1954, a virus named Wad Medani virus (WMV) was isolated from Hyalomma marginatum ticks from Maharashtra State, India. In 1963, another virus was isolated from Sturnia pagodarum birds in Tamil Nadu, India, and named Kammavanpettai virus (KVPTV) based on the site of its isolation. Originally these virus isolates could not be identified with conventional methods. Read More

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August 2018
1 Read
3.180 Impact Factor

Inactivated Piscine orthoreovirus vaccine protects against heart and skeletal muscle inflammation in Atlantic salmon.

J Fish Dis 2018 Sep 21;41(9):1411-1419. Epub 2018 Jun 21.

Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.

Heart- and skeletal muscle inflammation (HSMI) caused by infection with Piscine orthoreovirus (PRV) is one of the most common viral diseases in farmed Atlantic salmon (Salmo salar) in Norway, and disease outbreaks have been reported in most countries with large-scale Atlantic salmon aquaculture. Currently there is no vaccine available for protection against HSMI, partly due to the lack of a cell line for efficient virus propagation. Erythrocytes are the primary target cells for PRV in vivo and a potential source for isolation of PRV particles. Read More

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September 2018
11 Reads

First Complete Genome Sequence of Piscine Orthoreovirus Variant 3 Infecting Coho Salmon (Oncorhynchus kisutch) Farmed in Southern Chile.

Genome Announc 2018 Jun 14;6(24). Epub 2018 Jun 14.

Laboratorio de Diagnóstico y Biotecnología, ADL Diagnostic Chile SpA, Puerto Montt, Chile

We report here the complete genome of an isolate of piscine orthoreovirus variant 3 sequenced from a moribund coho salmon with jaundice that was reared in a seawater farm in southern Chile. The genome consists of 23,627 bp, including 10 segments that range from 1,052 bp (segment S4) to 4,014 bp (segment L1). Read More

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June 2018
5 Reads

Little evidence to suggest salmonid freshwater reservoirs of piscine orthoreovirus (PRV).

J Fish Dis 2018 Jun 11. Epub 2018 Jun 11.

Norwegian Veterinary Institute, Oslo, Norway.

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Reovirus Nonstructural Protein σNS Acts as an RNA Stability Factor Promoting Viral Genome Replication.

J Virol 2018 08 17;92(15). Epub 2018 Jul 17.

Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Viral nonstructural proteins, which are not packaged into virions, are essential for the replication of most viruses. Reovirus, a nonenveloped, double-stranded RNA (dsRNA) virus, encodes three nonstructural proteins that are required for viral replication and dissemination in the host. The reovirus nonstructural protein σNS is a single-stranded RNA (ssRNA)-binding protein that must be expressed in infected cells for production of viral progeny. Read More

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August 2018
1 Read

study on role of σB protein in avian reovirus pathogenesis.

Oncotarget 2018 Apr 13;9(28):19569-19583. Epub 2018 Apr 13.

Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.

Avian reoviruses, members of genus was known to cause diseases like tenosynovitis, runting-stunting syndrome in chickens. Among eight structural proteins, the proteins of S-class are mainly associated with viral arthritis but the significance of σB protein in arthritis is not established till date. In this infection pathological condition together with infection of joints often leads to arthritis because joints consists of cartilage which forms lubricating surface between two bones, and has limited metabolic, replicative and repair capacity. Read More

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April 2018
14 Reads
6.360 Impact Factor

Structural and Functional Features of the Reovirus σ1 Tail.

J Virol 2018 07 29;92(14). Epub 2018 Jun 29.

Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany

Mammalian orthoreovirus attachment to target cells is mediated by the outer capsid protein σ1, which projects from the virion surface. The σ1 protein is a homotrimer consisting of a filamentous tail, which is partly inserted into the virion; a body domain constructed from β-spiral repeats; and a globular head with receptor-binding properties. The σ1 tail is predicted to form an α-helical coiled coil. Read More

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July 2018
7 Reads

Development of a recombinant σB protein based dot-ELISA for the diagnosis of avian reovirus (ARV).

J Virol Methods 2018 07 13;257:69-72. Epub 2018 Apr 13.

Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, U.P., India. Electronic address:

Avian reovirus (ARV) causes significant economic losses to the poultry industry worldwide. The ARV proteins fall into three different classes based on their sizes:λ (large); μ (medium) and σ (small). σB, an outer capsid protein of the ARV contains group specific neutralizing epitopes and induces strong immune response in naturally infected chickens. Read More

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July 2018
1 Read

Development of a serum neutralization assay to detect Pteropine Orthoreovirus Indonesia/2010 neutralizing antibodies.

Vet Ital 2018 Apr 5. Epub 2018 Apr 5.

Giardino Zoologico di Pistoia, Via Pieve a Celle, Pistoia, Italy.

Pteropine Orthoreoviruses (PRVs) are fusogenic bat-borne orthoreoviruses that cause flu-like upper respiratory tract infections in humans. The presence of this group of viruses in bats and humans has been well documented in areas where their biological reservoirs - fruit bats (family Pteropodidae) - live densely. In the present study, a serum neutralization (SN) assay to detect neutralizing antibodies against PRV Indonesia/2010 isolate was set up and used to assess the seroprevalence of this virus in Italian domestic animals. Read More

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Molecular and Antigenic Characterization of Piscine orthoreovirus (PRV) from Rainbow Trout (Oncorhynchus mykiss).

Viruses 2018 04 2;10(4). Epub 2018 Apr 2.

Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 0454 Oslo, Norway.

(PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout () with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. Read More

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April 2018
2 Reads

Transcriptome Analysis of Epithelioma Papulosum Cyprini Cells Infected by Reovirus Isolated from Allogynogenetic Silver Crucian Carp.

Viruses 2018 03 18;10(3). Epub 2018 Mar 18.

Animal Science and Technology, Jilin Agriculture University, Changchun 130118, China.

The present study aimed to identify differentially expressed genes (DEGs) and major signal transduction pathways that were related to the immune response of epithelioma papulosum cyprinid (EPC) cells to reoviruses isolated from allogynogenetic silver crucian carp. The study also lays a theoretical foundation for the pathogenesis and immunity of the reovirus, which is helpful to the breeding of cyprinids fish. Reovirus infected and uninfected EPC cells were analyzed by using a new-generation high-throughput sequencing technology. Read More

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March 2018
2 Reads

Infectious Subviral Particle-induced Hemolysis Assay for Mammalian Orthoreovirus.

Bio Protoc 2018 Jan;8(2)

Department of Biology, Indiana University, Bloomington, Indiana, USA.

Mammalian orthoreovirus (reovirus) utilizes pore forming peptides to penetrate host cell membranes. This step is essential for delivering its genome containing core particle during viral entry. This protocol describes an assay for measuring reovirus-induced pore formation. Read More

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January 2018
1 Read

An Orchestra of Reovirus Receptors: Still Searching for the Conductor.

Adv Virus Res 2018 13;100:223-246. Epub 2017 Nov 13.

University of Pittsburgh School of Medicine, Pittsburgh, PA, United States. Electronic address:

Viruses are constantly engaged in a molecular arms race with the host, where efficient and tactical use of cellular receptors benefits critical steps in infection. Receptor use dictates initiation, establishment, and spread of viral infection to new tissues and hosts. Mammalian orthoreoviruses (reoviruses) are pervasive pathogens that use multiple receptors to overcome protective host barriers to disseminate from sites of initial infection and cause disease in young mammals. Read More

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November 2017

Piscine orthoreovirus infection in Atlantic salmon (Salmo salar) protects against subsequent challenge with infectious hematopoietic necrosis virus (IHNV).

Vet Res 2018 03 13;49(1):30. Epub 2018 Mar 13.

National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.

Infectious hematopoietic necrosis virus (IHNV) is endemic in farmed rainbow trout in continental Europe and in various salmonid fish species at the Pacific coast of North America. IHN has never occurred in European Atlantic salmon (Salmo salar) farms, but is considered as a major threat for the European salmon industry. Another virus, Piscine orthoreovirus (PRV), is widespread in the sea phase of Atlantic salmon, and is identified as the causative agent of heart and skeletal muscle inflammation. Read More

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March 2018
4 Reads

Reovirus-Induced Apoptosis in the Intestine Limits Establishment of Enteric Infection.

J Virol 2018 05 27;92(10). Epub 2018 Apr 27.

Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Several viruses induce intestinal epithelial cell death during enteric infection. However, it is unclear whether proapoptotic capacity promotes or inhibits replication in this tissue. We infected mice with two reovirus strains that infect the intestine but differ in the capacity to alter immunological tolerance to new food antigen. Read More

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May 2018
5 Reads

High-Resolution Screening of Viral Communities and Identification of New Pathogens in Fish Using Next-Generation Sequencing.

Methods Mol Biol 2018 ;1746:151-159

Bioinformatics department, BaseClear B.V., Leiden, The Netherlands.

Discovery of viral genomes in fish has historically been based on viral enrichment, random priming, cloning, and Sanger sequencing. However, the development of next-generation sequencing has enabled the possibility to sequence the entire virome of a tissue sample. This has led to an enormous increase in discovery of new viruses. Read More

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January 2018
1 Read

Construction and characterization of an infectious molecular clone of novel duck reovirus.

J Gen Virol 2018 Apr 26;99(4):449-456. Epub 2018 Feb 26.

National Engineering Research Center for Poultry, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, PR China.

Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is currently an infectious agent for ducks. Studies on NDRV replication and pathogenesis have been hampered by the lack of an available reverse-genetics system. In this study, a plasmid-based reverse-genetics system that is free of helper viruses has been developed. Read More

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April 2018
5 Reads

The dynamics of both filamentous and globular mammalian reovirus viral factories rely on the microtubule network.

Virology 2018 05 20;518:77-86. Epub 2018 Feb 20.

Dept. of Molecular Genetics and Immunobiology, Harvard Medical School, Harvard University, 77 Av. Louis Pasteur, Boston, MA 02115, USA. Electronic address:

Mammalian reovirus viral factories (VFs) form filamentous or globular structures depending on the viral strain. In this study, we attempt to characterize the dynamics of both filamentous and globular VFs. Here, we present evidence demonstrating that globular VFs are dynamic entities coalescing between them, thereby gaining in size and concomitantly decreasing in numbers during the course of the infection. Read More

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May 2018
3 Reads

Differential Delivery of Genomic Double-Stranded RNA Causes Reovirus Strain-Specific Differences in Interferon Regulatory Factor 3 Activation.

J Virol 2018 05 13;92(9). Epub 2018 Apr 13.

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

Serotype 3 (T3) reoviruses induce substantially more type 1 interferon (IFN-I) secretion than serotype 1 (T1) strains. However, the mechanisms underlying differences in IFN-I production between T1 and T3 reoviruses remain undefined. Here, we found that differences in IFN-I production between T1 and T3 reoviruses correlate with activation of interferon regulatory factor 3 (IRF3), a key transcription factor for the production of IFN-I. Read More

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May 2018
3 Reads

Reducing uncertainty in estimating virus reduction by advanced water treatment processes.

Water Res 2018 04 3;133:282-288. Epub 2018 Feb 3.

University of Arizona, Department of Soil, Water and Environmental Science, Maricopa Agricultural Center, 37860, W. Smith-Enke Road, Maricopa, AZ 85138, USA. Electronic address:

Treatment of wastewater for potable reuse requires the reduction of enteric viruses to levels that pose no significant risk to human health. Advanced water treatment trains (e.g. Read More

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April 2018
28 Reads

Infectious Subviral Particle to Membrane Penetration Active Particle (ISVP-to-ISVP*) Conversion Assay for Mammalian Orthoreovirus.

Bio Protoc 2018 Jan;8(2)

Department of Biology, Indiana University, Bloomington, Indiana, USA.

The mammalian orthoreovirus (reovirus) outer capsid undergoes a series of conformational changes prior to or during viral entry. These transitions are necessary for delivering the genome-containing core across host cell membranes. This protocol describes an assay for monitoring the transition into a membrane penetration-active form (, ISVP*). Read More

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January 2018