1,871 results match your criteria Reoviruses


Discovery and characterization of a novel insect-specific reovirus isolated from .

J Gen Virol 2020 May 29. Epub 2020 May 29.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.

A novel double-stranded RNA (dsRNA) virus designated reovirus (PARV) was found in the leafhopper in China. Spherical particles approximately 70 nm in diameter arranged in a crystalline array were observed in the salivary gland tissues of infected leafhoppers by transmission electron microscopy. Some viral particles were also encased in tubules, similar to those of previously described reoviruses. Read More

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http://dx.doi.org/10.1099/jgv.0.001442DOI Listing

A viral fusogen hijacks the actin cytoskeleton to drive cell-cell fusion.

Elife 2020 May 22;9. Epub 2020 May 22.

UC Berkeley-UC San Francisco Graduate Group in Bioengineering, Berkeley, United States.

Cell-cell fusion, which is essential for tissue development and used by some viruses to form pathological syncytia, is typically driven by fusogenic membrane proteins with tall (>10 nm) ectodomains that undergo conformational changes to bring apposing membranes in close contact prior to fusion. Here we report that a viral fusogen with a short (<2 nm) ectodomain, the reptilian orthoreovirus p14, accomplishes the same task by hijacking the actin cytoskeleton. We show that phosphorylation of the cytoplasmic domain of p14 triggers N-WASP-mediated assembly of a branched actin network. Read More

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http://dx.doi.org/10.7554/eLife.51358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244324PMC

Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats () in Singapore.

Viruses 2020 May 14;12(5). Epub 2020 May 14.

Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. Read More

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http://dx.doi.org/10.3390/v12050539DOI Listing

The dynamics of molecular evolution of emerging avian reoviruses through accumulation of point mutations and genetic re-assortment.

Virus Evol 2020 Jan 10;6(1):veaa025. Epub 2020 May 10.

Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada.

In the last decade, the emergence of variant strains of avian reovirus (ARV) has caused enormous economic impact in the poultry industry across Canada and USA. ARVs are non-enveloped viruses with ten segments of double-stranded RNA genome. So far, only six genotyping cluster groups are identified worldwide based on sequence analysis of the σC protein encoded by the S1 segment. Read More

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http://dx.doi.org/10.1093/ve/veaa025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211400PMC
January 2020

Sequencing and phylogenetic analysis of partial S1 genes of avian orthoreovirus isolates in Shandong province during 2015-2017.

Poult Sci 2020 May 28;99(5):2416-2423. Epub 2020 Feb 28.

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 276018, People's Republic of China. Electronic address:

Outbreaks of avian orthoreovirus (ARV) infection with primary symptoms of arthritis/tenosynovitis syndrome have been occurring more frequently in broiler flocks in China in recent years. This study aimed to investigate the genetic characteristics of ARV field strains in broiler flocks exhibiting arthritis/tenosynovitis syndrome from 9 cities in Shandong province during 2015 to 2017. A total of 64 synovial and tendon samples were obtained from broilers with significant arthritis/tenosynovitis syndrome, and 21 ARV field strains were obtained. Read More

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http://dx.doi.org/10.1016/j.psj.2019.11.067DOI Listing

A modified lysosomal organelle mediates nonlytic egress of reovirus.

J Cell Biol 2020 Jul;219(7)

Cell Structure Laboratory, National Center for Biotechnology, Spanish National Research Council, Madrid, Spain.

Mammalian orthoreoviruses (reoviruses) are nonenveloped viruses that replicate in cytoplasmic membranous organelles called viral inclusions (VIs) where progeny virions are assembled. To better understand cellular routes of nonlytic reovirus exit, we imaged sites of virus egress in infected, nonpolarized human brain microvascular endothelial cells (HBMECs) and observed one or two distinct egress zones per cell at the basal surface. Transmission electron microscopy and 3D electron tomography (ET) of the egress zones revealed clusters of virions within membrane-bound structures, which we term membranous carriers (MCs), approaching and fusing with the plasma membrane. Read More

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http://dx.doi.org/10.1083/jcb.201910131DOI Listing

A method for the unbiased quantification of reassortment in segmented viruses.

J Virol Methods 2020 Jun 28;280:113878. Epub 2020 Apr 28.

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, United States; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), United States. Electronic address:

Reassortment of segmented viruses can be an important source of genetic diversity underlying viral evolution and emergence. Methods for the quantification of reassortment have been described but are often cumbersome and best suited for the analysis of reassortment between highly divergent parental strains. While it is useful to understand the potential of divergent parents to reassort, outcomes of such heterologous reassortment are driven by differential selection acting on the progeny and are typically strain specific. Read More

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http://dx.doi.org/10.1016/j.jviromet.2020.113878DOI Listing

Isolation and identification of two new strains of mammalian orthoreovirus from Chinese tree shrews.

Arch Virol 2020 Apr 25. Epub 2020 Apr 25.

Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, 935 Jiao Ling Road, Kunming, China.

Chinese tree shrews have been used extensively in studies of different types of cancer and for the modeling of viral infections. In the present study, we report the isolation and characterization of two strains of mammalian orthoreovirus (MRV), MRV1/TS/2011 and MRV3/TS/2012, which were isolated from the feces of tree shrews in Yunnan, China. These two strains of MRV were isolated and cultured in both primary tree shrew intestinal epithelial cells (pTIECs) and primary tree shrew alveolar epithelial cells (pTAECs). Read More

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http://dx.doi.org/10.1007/s00705-020-04635-1DOI Listing

Immunopathological characterization of red focal changes in Atlantic salmon (Salmo salar) white muscle.

Vet Immunol Immunopathol 2020 Apr 12;222:110035. Epub 2020 Mar 12.

Department of Basic Science and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway. Electronic address:

Farmed Atlantic salmon (Salmo salar) are prone to various conditions affecting the quality of the fillet. A well-known but so far poorly understood condition is the focal red changes in muscle, often referred to as haemorrhages. Such changes are characterized by muscle necrosis, haemorrhages and acute inflammation. Read More

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http://dx.doi.org/10.1016/j.vetimm.2020.110035DOI Listing

Viral Vector-Based Melanoma Gene Therapy.

Biomedicines 2020 Mar 16;8(3). Epub 2020 Mar 16.

PanTherapeutics, CH 1095 Lutry, Switzerland.

Gene therapy applications of oncolytic viruses represent an attractive alternative for cancer treatment. A broad range of oncolytic viruses, including adenoviruses, adeno-associated viruses, alphaviruses, herpes simplex viruses, retroviruses, lentiviruses, rhabdoviruses, reoviruses, measles virus, Newcastle disease virus, picornaviruses and poxviruses, have been used in diverse preclinical and clinical studies for the treatment of various diseases, including colon, head-and-neck, prostate and breast cancer as well as squamous cell carcinoma and glioma. The majority of studies have focused on immunotherapy and several drugs based on viral vectors have been approved. Read More

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http://dx.doi.org/10.3390/biomedicines8030060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148454PMC

Interaction between Translocation-associated membrane protein 1 and σC protein of novel duck reovirus controls virus infectivity.

Virus Genes 2020 Jun 16;56(3):347-353. Epub 2020 Mar 16.

National Engineering Research Center for Poultry, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai, 200241, China.

Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectrometry found that σC coprecipitated with some host proteins including Translocation-associated membrane protein 1 (TRAM1). Read More

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http://dx.doi.org/10.1007/s11262-020-01750-8DOI Listing

Author Correction: Piscine orthoreovirus demonstrates high infectivity but low virulence in Atlantic salmon of Pacific Canada.

Sci Rep 2020 Mar 10;10(1):4731. Epub 2020 Mar 10.

Pacific Biological Station, Fisheries and Oceans Canada, Nanaimo, V9T 6N7, Canada.

An amendment to this paper has been published and can be accessed via a link at the top of the paper. Read More

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http://dx.doi.org/10.1038/s41598-020-61781-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062880PMC

Pathogenicity of a variant duck orthoreovirus strain in Cherry Valley Ducklings.

Vet Microbiol 2020 Mar 3;242:108546. Epub 2019 Dec 3.

College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, China. Electronic address:

Since 2017, a disease that is characterized by spleen necrosis and swelling has emerged in China's main meat duck breeding provinces, this disease generally causes a large number of ducks to develop a poor mental state and either an increase or loss of appetite, as well as potentially resulting in death. Necrosis of spleen in this disease weakens the duck's immunity, therefore often leading to secondary infection. The net result of this is significant economic loss to China's duck breeding industry. Read More

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http://dx.doi.org/10.1016/j.vetmic.2019.108546DOI Listing

Dissemination of (PRV-1) in Atlantic Salmon () during the Early and Regenerating Phases of Infection.

Pathogens 2020 Feb 20;9(2). Epub 2020 Feb 20.

Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, 0454 Oslo, Norway.

(PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Read More

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http://dx.doi.org/10.3390/pathogens9020143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169402PMC
February 2020

Novel human reovirus isolated from children and its long-term circulation with reassortments.

Sci Rep 2020 Jan 22;10(1):963. Epub 2020 Jan 22.

Division of Public Health, Osaka Institute of Public Health, Osaka, 537-0025, Japan.

Mammalian orthoreovirus (MRV), also known as reovirus, was discovered in the 1950s and became the first reported segmented double-stranded RNA virus. MRVs have since been found in a variety of animal species, including humans. However, reports on MRV infections are scarce due to the rarity of their symptomatic occurrence. Read More

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http://dx.doi.org/10.1038/s41598-020-58003-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6976588PMC
January 2020

Partial Molecular Characterization and Pathogenicity Study of an Avian Reovirus Causing Tenosynovitis in Commercial Broilers.

Avian Dis 2019 09;63(3):452-460

Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616,

This study describes the molecular characterization of avian reoviruses (ARVs) isolated during an outbreak in commercial chickens between 2015 and 2016. In addition, a pathogenicity study of a selected ARV strain isolated from a field case of viral tenosynovitis in commercial broiler chickens was performed. On the basis of phylogenetic analysis of a 1088-bp fragment of the ARV S1 gene, the investigated sequences were differentiated into five distinct genotypic clusters (GCs), namely GC1, GC2, GC3, GC4, and GC6. Read More

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http://dx.doi.org/10.1637/12013-121418-Reg.1DOI Listing
September 2019

Forces during cellular uptake of viruses and nanoparticles at the ventral side.

Nat Commun 2020 01 2;11(1):32. Epub 2020 Jan 2.

Max Planck Institute for Medical Research, Jahnstraße 29, 69120, Heidelberg, Germany.

Many intracellular pathogens, such as mammalian reovirus, mimic extracellular matrix motifs to specifically interact with the host membrane. Whether and how cell-matrix interactions influence virus particle uptake is unknown, as it is usually studied from the dorsal side. Here we show that the forces exerted at the ventral side of adherent cells during reovirus uptake exceed the binding strength of biotin-neutravidin anchoring viruses to a biofunctionalized substrate. Read More

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http://dx.doi.org/10.1038/s41467-019-13877-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940367PMC
January 2020

Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (Rousettus aegyptiacus).

J Vet Med Sci 2020 Feb 20;82(2):162-167. Epub 2019 Dec 20.

Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan.

Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Read More

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http://dx.doi.org/10.1292/jvms.19-0424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041985PMC
February 2020

Kinetics of Oncolytic Reovirus T3D Replication and Growth Pattern in Mesenchymal Stem Cells.

Cell J 2020 Oct 15;22(3):283-292. Epub 2019 Dec 15.

Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA.

Objective: Currently, application of oncolytic-virus in cancer treatment of clinical trials are growing. Oncolytic-reovirus is an attractive anti-cancer therapeutic agent for clinical testing. Many studies used mesenchymal stem cells (MSCs) as a carrier cell to enhance the delivery and quality of treatment with oncolytic-virotherapy. Read More

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http://dx.doi.org/10.22074/cellj.2020.6686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947011PMC
October 2020

Reviewing the risk of feed as a vehicle for swine pathogen transmission.

Vet Med Sci 2019 Dec 18. Epub 2019 Dec 18.

Department of Grain Science and Industry, Kansas State University, Manhattan, KS, USA.

Background: While porcine biological hazards have had the potential to be transmitted through feed and feed mills for decades, the emerging threat of foreign animal disease has elevated the concern that these may enter or be transmitted throughout the domestic swine herd via a feed vehicle.

Objective: The goal of this review was to describe the current classification for emerging porcine biological pathogen transmission through the feed supply chain so resources can be best directed towards those of highest risk.

Methods: By assessing the pathogen severity to pigs and the probability of pathogen transmission through feed, an overall risk can be established using a hazard analysis matrix. Read More

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http://dx.doi.org/10.1002/vms3.227DOI Listing
December 2019

First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil.

Sci Rep 2019 12 9;9(1):18599. Epub 2019 Dec 9.

Institute of Biological Sciences, Federal University of Para, Para, 66075-000, Brazil.

Diarrhea remains one of the most common causes of deaths in children. Although many studies have investigated the prevalence of enteric pathogens around the globe some diarrheal episodes remain unexplained. It is possible that some yet-unidentified viral agents could be related to these cases of gastroenteritis. Read More

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http://dx.doi.org/10.1038/s41598-019-55216-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901473PMC
December 2019
5.078 Impact Factor

Molecular detection of Mycoplasma synoviae and avian reovirus infection in arthritis and tenosynovitis lesions of broiler and breeder chickens in Santa Catarina State, Brazil.

J S Afr Vet Assoc 2019 Nov 29;90(0):e1-e5. Epub 2019 Nov 29.

Department of Animal Production, Center for Agricultural Sciences, Santa Catarina State University, Lages, Brazil; and, VERTÀ, Research and Veterinary Diagnostic Institute, Curitibanos, Santa Catarina.

Infectious arthritis or tenosynovitis in broiler and breeder chickens results in major loss of productivity because of reduced growth and downgrading at processing plants. The most common causative agents of avian infectious arthritis are the bacterium Mycoplasma synoviae and avian reoviruses (ARVs) (family Reoviridae, genus Orthoreovirus). In this study, we evaluated the occurrence of these two pathogens in arthritis or tenosynovitis lesions of broilers and breeder flocks in southern Brazil using molecular detection. Read More

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http://dx.doi.org/10.4102/jsava.v90i0.1970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893155PMC
November 2019

What are the limits of the packaging capacity for genomic RNA in the cores of rotaviruses and of other members of the Reoviridae?

Virus Res 2020 Jan 26;276:197822. Epub 2019 Nov 26.

Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK. Electronic address:

Species A rotaviruses (RVAs) can package additional homologous genomic sequences which have emerged by partial duplication events (genome rearrangements). Due to the availability of a plasmid only-based reverse genetics system for RVs, various heterologous sequences have been rescued as fusion genes into viable RVAs. Using cryo-electron microscopy, the native structures of the dsRNA genome of Cytoplasmic Polyhedrosis Virus, a member of the Cypovirus genus of the Reoviridae, and partially of Rotavirus and Orbivirus, have been determined. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.197822DOI Listing
January 2020
5 Reads

[Molecular Mechanisms of Apoptosis of Leukemia Cell Induced by Reovirus].

Sichuan Da Xue Xue Bao Yi Xue Ban 2019 Sep;50(5):649-653

Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of Medical Sciences, Guiyang 550004, China.

Objective: To investigate the molecular mechanism of apoptosis of HL60 cells induced by oncolytic virus Reovirus type 3 (Reo3).

Methods: HL60 cells were infected with Reo3 at different multiplicity of infection (MOI) with the uninfected HL60 cells as control group. After 48 h of infection, the activity of HL60 cells infected with virus at different MOI was detected by CCK8 method to investigate the influence of MOI to cell activity. Read More

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September 2019

Single Amino Acid Differences between Closely Related Reovirus T3D Lab Strains Alter Oncolytic Potency and .

J Virol 2020 Jan 31;94(4). Epub 2020 Jan 31.

Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada

Little is known about how genetic variations in viruses affect their success as therapeutic agents. The type 3 Dearing strain of (T3D) is undergoing clinical trials as an oncolytic virotherapy. Worldwide, studies on reovirus oncolysis use T3D stocks propagated in different laboratories. Read More

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http://dx.doi.org/10.1128/JVI.01688-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997766PMC
January 2020

Review of infectious agent occurrence in wild salmonids in British Columbia, Canada.

J Fish Dis 2020 Feb 19;43(2):153-175. Epub 2019 Nov 19.

Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.

Wild Pacific salmonids (WPS) are economically and culturally important to the Pacific North region. Most recently, some populations of WPS have been in decline. Of hypothesized factors contributing to the decline, infectious agents have been postulated to increase the risk of mortality in Pacific salmon. Read More

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http://dx.doi.org/10.1111/jfd.13084DOI Listing
February 2020

Six Nearly Complete Genome Segments of a Novel Reovirus Identified in Laotian Batflies.

Microbiol Resour Announc 2019 Nov 14;8(46). Epub 2019 Nov 14.

Institut Pasteur, Biology of Infection Unit, Inserm U1117, Pathogen Discovery Laboratory, Paris, France

As part of the characterization of viral communities of Laotian batflies, we report here the sequencing of six nearly complete genome segments of a novel reovirus identified in Laotian batflies that is distantly related to reoviruses recently reported in various Diptera species. Read More

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http://dx.doi.org/10.1128/MRA.00733-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856264PMC
November 2019

Corrigendum: High-Load Reovirus Infections Do Not Imply Physiological Impairment in Salmon.

Front Physiol 2019;10:1354. Epub 2019 Oct 29.

Aquatic Diagnostics and Genomics Division, Pacific Biological Station, Fisheries and Oceans Canada, Nanaimo, BC, Canada.

[This corrects the article DOI: 10.3389/fphys.2019. Read More

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http://dx.doi.org/10.3389/fphys.2019.01354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830122PMC
October 2019

First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt.

Trop Anim Health Prod 2020 May 8;52(3):1049-1054. Epub 2019 Nov 8.

Department of Animal Medicine, Faculty of Veterinary Medicine, Alexandria University, Edfina, 22758, Egypt.

Recently, the Egyptian broiler industry has experienced an increased incidence of avian reovirus (ARV) infections. However, to date, no studies have been carried out to investigate the epidemiologic status of ARV infections as well as the genetic characteristics of the currently circulating ARV strains. The present study estimates the seroprevalence of ARV infections in Alexandria, El-Behera, Giza, Kafr El-Sheikh, and Gharbia governorates, Egypt, during the period 2017-2018. Read More

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http://dx.doi.org/10.1007/s11250-019-02100-zDOI Listing

Proliferative Kidney Disease and Proliferative Darkening Syndrome are Linked with Brown Trout () Mortalities in the Pre-Alpine Isar River.

Pathogens 2019 Oct 6;8(4). Epub 2019 Oct 6.

Institute for Infectious Diseases and Zoonoses, LMU Munich, 80539 Munich, Germany.

For many years, brown trout () mortalities within the pre-alpine Isar River in Germany were reported by the Bavarian Fisheries Association (Landesfischereiverband Bayern e.V.) and local recreational anglers during August and September. Read More

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http://dx.doi.org/10.3390/pathogens8040177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963635PMC
October 2019

Current status of fish vaccines in Japan.

Fish Shellfish Immunol 2019 Dec 3;95:236-247. Epub 2019 Oct 3.

Research Center of Fish Diseases, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Minami.-Ise, Mie, Japan. Electronic address:

Aquaculture is an important industry in Japan for the sustainable production of fish. It contributes to the diversity of Japanese traditional food culture, which uses fish such as "sushi" and "sashimi". In the recent aquaculture setting in Japan, infectious diseases have been an unavoidable problem and have caused serious economic losses. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.09.031DOI Listing
December 2019

Glycan-mediated enhancement of reovirus receptor binding.

Nat Commun 2019 10 1;10(1):4460. Epub 2019 Oct 1.

Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.

Viral infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and their cell surface receptors. Despite recent progress, the molecular mechanisms underlying the multistep reovirus entry process are poorly understood. Read More

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http://dx.doi.org/10.1038/s41467-019-12411-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773860PMC
October 2019
1 Read

Detection of Salmonid IgM Specific to the Outer Capsid Spike Protein Sigma 1 Using Lipid-Modified Antigens in a Bead-Based Antibody Detection Assay.

Front Immunol 2019 6;10:2119. Epub 2019 Sep 6.

Department of Fish Health, Norwegian Veterinary Institute, Oslo, Norway.

Bead-based multiplex immunoassays are promising tools for determination of the specific humoral immune response. In this study, we developed a multiplexed bead-based immunoassay for the detection of Atlantic salmon () antibodies against (PRV). Three different genotypes of PRV (PRV-1, PRV-2, and PRV-3) cause disease in farmed salmonids. Read More

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http://dx.doi.org/10.3389/fimmu.2019.02119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743345PMC
September 2019
2 Reads

Hypericin-loaded graphene oxide protects ducks against a novel duck reovirus.

Mater Sci Eng C Mater Biol Appl 2019 Dec 4;105:110052. Epub 2019 Aug 4.

Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China. Electronic address:

Novel duck reovirus (NDRV) disease is a serious infectious disease for poultry, for which no effective therapy has been established. Therefore, development of novel antivirals against NDRV is urgently needed. In present study, we developed a complex wherein hypericin (HY), which shows broad-spectrum antiviral activity, was loaded onto graphene oxide (GO), which has a high drug-loading capacity and low cytotoxicity. Read More

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http://dx.doi.org/10.1016/j.msec.2019.110052DOI Listing
December 2019
3.088 Impact Factor

Enhanced Killing of Triple-Negative Breast Cancer Cells by Reassortant Reovirus and Topoisomerase Inhibitors.

J Virol 2019 12 13;93(23). Epub 2019 Nov 13.

Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA

Breast cancer is the second leading cause of cancer-related deaths in women in the United States. Triple-negative breast cancer constitutes a subset of breast cancer that is associated with higher rates of relapse, decreased survival, and limited therapeutic options for patients afflicted with this type of breast cancer. Mammalian orthoreovirus (reovirus) selectively infects and kills transformed cells, and a serotype 3 reovirus is in clinical trials to assess its efficacy as an oncolytic agent against several cancers. Read More

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http://dx.doi.org/10.1128/JVI.01411-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6854488PMC
December 2019
1 Read
4.439 Impact Factor

Erythroid Progenitor Cells in Atlantic Salmon () May Be Persistently and Productively Infected with Piscine Orthoreovirus (PRV).

Viruses 2019 Sep 5;11(9). Epub 2019 Sep 5.

Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 0454 Oslo, Norway.

Piscine orthoreovirus (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (). The virus targets erythrocytes in the acute peak phase, followed by cardiomyocytes, before the infection subsides into persistence. The persistent phase is characterized by high level of viral RNA, but low level of viral protein. Read More

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http://dx.doi.org/10.3390/v11090824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784031PMC
September 2019
1 Read

Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II.

Microb Pathog 2019 Nov 3;136:103715. Epub 2019 Sep 3.

College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.

The rare minnow, Gobiocypris rarus, is small experimental fish proven to be sensitive to Grass Carp Reovirus (GCRV) infection. In present study we established a new cell (GrE) from eggs of G. rarus. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08824010193094
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http://dx.doi.org/10.1016/j.micpath.2019.103715DOI Listing
November 2019
5 Reads
2.000 Impact Factor

Breast Tumor-Associated Metalloproteases Restrict Reovirus Oncolysis by Cleaving the σ1 Cell Attachment Protein and Can Be Overcome by Mutation of σ1.

J Virol 2019 11 29;93(22). Epub 2019 Oct 29.

Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta, Canada

Reovirus is undergoing clinical testing as an oncolytic therapy for breast cancer. Given that reovirus naturally evolved to thrive in enteric environments, we sought to better understand how breast tumor microenvironments impinge on reovirus infection. Reovirus was treated with extracellular extracts generated from polyomavirus middle T-antigen-derived mouse breast tumors. Read More

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http://dx.doi.org/10.1128/JVI.01380-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819916PMC
November 2019

Entry of bunyaviruses into plants and vectors.

Adv Virus Res 2019 9;104:65-96. Epub 2019 Aug 9.

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. Electronic address:

The majority of plant-infecting viruses are transmitted by arthropod vectors that deliver them directly into a living plant cell. There are diverse mechanisms of transmission ranging from direct binding to the insect stylet (non-persistent transmission) to persistent-propagative transmission in which the virus replicates in the insect vector. Despite this diversity in interactions, most arthropods that serve as efficient vectors have feeding strategies that enable them to deliver the virus into the plant cell without extensive damage to the plant and thus effectively inoculate the plant. Read More

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http://dx.doi.org/10.1016/bs.aivir.2019.07.001DOI Listing
December 2019

Molecular characterization of avian reovirus causing tenosynovitis outbreaks in broiler flocks, Iran.

Avian Pathol 2020 Feb 15;49(1):15-20. Epub 2019 Aug 15.

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Avian reoviruses (ARVs) cause arthritis, tenosynovitis, retarded growth, and malabsorption syndrome. After a long time of effective prevention and low rates of viral arthritis/ tenosynovitis in Iran, outbreaks of tenosynovitis in broiler flocks have increased in recent years. Lameness, splay legs, high rate of cull birds, poor performance, uneven birds at harvest, and condemnation at processing cause huge economic losses. Read More

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http://dx.doi.org/10.1080/03079457.2019.1654086DOI Listing
February 2020
7 Reads

Gamma Interferon Alters Junctional Integrity via Rho Kinase, Resulting in Blood-Brain Barrier Leakage in Experimental Viral Encephalitis.

mBio 2019 08 6;10(4). Epub 2019 Aug 6.

Department of Pediatrics, Section of Developmental Biology, University of Colorado, School of Medicine, Aurora, Colorado, USA

Blood-brain barrier (BBB) breakdown is a hallmark of many diseases of the central nervous system (CNS). Loss of BBB integrity in CNS diseases such as viral encephalitis results in the loss of nutrient/oxygen delivery, rapid infiltration of immune cells, and brain swelling that can exacerbate neuronal injury. Despite this, the cellular and molecular mechanisms that underlie BBB breakdown in viral encephalitis are incompletely understood. Read More

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http://dx.doi.org/10.1128/mBio.01675-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686045PMC
August 2019
1 Read

Heterogeneous Nuclear Ribonucleoprotein A1 and Lamin A/C Modulate Nucleocytoplasmic Shuttling of Avian Reovirus p17.

J Virol 2019 10 30;93(20). Epub 2019 Sep 30.

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan

Avian reovirus (ARV) p17 protein continuously shuttles between the nucleus and the cytoplasm via transcription-dependent and chromosome region maintenance 1 (CRM1)-independent mechanisms. Nevertheless, whether cellular proteins modulate nucleocytoplasmic shuttling of p17 remains unknown. This is the first report that heterogeneous nuclear ribonucleoprotein (hnRNP) A1 serves as a carrier protein to modulate nucleocytoplasmic shuttling of p17. Read More

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http://dx.doi.org/10.1128/JVI.00851-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798114PMC
October 2019

Directed evolution as a tool for the selection of oncolytic RNA viruses with desired phenotypes.

Oncolytic Virother 2019 12;8:9-26. Epub 2019 Jul 12.

SATOR Therapeutics LLC , Cleveland, OH 44106, USA.

Viruses have some characteristics in common with cell-based life. They can evolve and adapt to environmental conditions. Directed evolution can be used by researchers to produce viral strains with desirable phenotypes. Read More

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http://dx.doi.org/10.2147/OV.S176523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6636189PMC
July 2019
2 Reads

The effects of autophagy on the replication of Nelson Bay orthoreovirus.

Virol J 2019 07 18;16(1):90. Epub 2019 Jul 18.

Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.

Background: Nelson Bay orthoreovirus (NBV) was first isolated over 40 years ago from a fruit bat in Australia. Normally, NBV does not cause human diseases, but recently several NBV strains have been associated with human respiratory tract infections, thus attracting clinical attention. Autophagy, an evolutionarily conserved process in eukaryotic cells, degrades intracellular substrates, participates in multiple physiological processes, and maintains cellular homeostasis. Read More

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http://dx.doi.org/10.1186/s12985-019-1196-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6639940PMC
July 2019
5 Reads

Fusogenic Reoviruses and Their Fusion-Associated Small Transmembrane (FAST) Proteins.

Authors:
Roy Duncan

Annu Rev Virol 2019 09 5;6(1):341-363. Epub 2019 Jul 5.

Department of Microbiology & Immunology, Department of Biochemistry & Molecular Biology, and Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada, B3H 4R2; email:

With no limiting membrane surrounding virions, nonenveloped viruses have no need for membrane fusion to gain access to intracellular replication compartments. Consequently, nonenveloped viruses do not encode membrane fusion proteins. The only exception to this dogma is the fusogenic reoviruses that encode fusion-associated small transmembrane (FAST) proteins that induce syncytium formation. Read More

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http://dx.doi.org/10.1146/annurev-virology-092818-015523DOI Listing
September 2019

Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10.8-induced cell cycle arrest and apoptosis.

Vet Microbiol 2019 Aug 28;235:151-163. Epub 2019 Jun 28.

Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan; The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan; Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; Ph. D Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan. Electronic address:

This study demonstrates that the Muscovy duck reovirus (MDRV) p10.8 protein is one of many viral non-structural proteins that induces both cell cycle arrest and apoptosis. The p10. Read More

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http://dx.doi.org/10.1016/j.vetmic.2019.06.017DOI Listing
August 2019
1 Read

Altered gene expression profiles of the MDA5 signaling pathway in peripheral blood lymphocytes of chickens infected with avian reovirus.

Arch Virol 2019 Oct 4;164(10):2451-2458. Epub 2019 Jul 4.

Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, People's Republic of China.

Avian reovirus (ARV) is a member of the genus Orthoreovirus in the family Reoviridae and causes a severe syndrome including viral arthritis that leads to considerable losses in the poultry industry. Innate immunity plays a significant role in host defense against ARV. Here, we explored the interaction between ARV and the host innate immune system by measuring mRNA expression levels of several genes associated with the MDA5 signaling pathway. Read More

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http://dx.doi.org/10.1007/s00705-019-04340-8DOI Listing
October 2019
4 Reads

Genotypic Characterization of Emerging Avian Reovirus Genetic Variants in California.

Sci Rep 2019 06 27;9(1):9351. Epub 2019 Jun 27.

University of California, Davis, School of Veterinary Medicine, Davis, 95616, CA, United States.

This study focuses on virus isolation of avian reoviruses from a tenosynovitis outbreak between September 2015 and June 2018, the molecular characterization of selected isolates based on partial S1 gene sequences, and the full genome characterization of seven isolates. A total of 265 reoviruses were detected and isolated, 83.3% from tendons and joints, 12. Read More

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http://dx.doi.org/10.1038/s41598-019-45494-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597705PMC
June 2019
3 Reads

SEROLOGICAL SURVEY FOR SELECT INFECTIOUS AGENTS IN WILD MAGELLANIC PENGUINS () IN ARGENTINA, 1994-2008.

J Wildl Dis 2020 Jan 25;56(1):66-81. Epub 2019 Jun 25.

Field Veterinary Program, Wildlife Conservation Society, 2300 Southern Boulevard, Bronx, New York 10460, USA.

Despite being the most numerous penguin species in South America, exposure of the Magellanic Penguin () to pathogens has not yet been thoroughly assessed. We collected serum from 1,058 Magellanic Penguins at 10 breeding colonies along the entire latitudinal range of this species in Argentina. The work spanned 10 breeding seasons over 15 yr (1994-2008). Read More

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January 2020
16 Reads