408,509 results match your criteria Regulatory Peptides[Journal]


Functional expansion of a TCA cycle operon mRNA by a 3' end-derived small RNA.

Nucleic Acids Res 2018 Dec 12. Epub 2018 Dec 12.

RNA Biology Group, Institute for Molecular Infection Biology, University of Würzburg, D-97080 Würzburg, Germany.

Global RNA profiling studies in bacteria have predicted the existence of many of small noncoding RNAs (sRNAs) that are processed off mRNA 3' ends to regulate other mRNAs via the RNA chaperones Hfq and ProQ. Here, we present targets of SdhX (RybD), an Hfq-dependent sRNA that is generated by RNase E mediated 3' processing of the ∼10 000-nt mRNA of the TCA cycle operon sdhCDAB-sucABCD in enteric bacteria. An in silico search predicted ackA mRNA, which encodes acetate kinase, as a conserved primary target of SdhX. Read More

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December 2018

DNA demethylation facilitates the specific transcription of the mouse X-linked Tsga8 gene in round spermatids.

Biol Reprod 2018 Dec 12. Epub 2018 Dec 12.

Department of Medical Genetics and Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan Province, China.

Some X-linked genes necessary for spermiogenesis are specifically activated in the post-meiotic germ cells. However, the regulatory mechanism about this activation is not clearly understood. Here, we examined the potential mechanism controlling the transcriptional activation of the mouse testis specific gene A8 (Tsga8) gene in round spermatids. Read More

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December 2018

Gene expression models based on transcription factor binding events confer insight into functional cis-regulatory variants.

Bioinformatics 2018 Dec 12. Epub 2018 Dec 12.

Centre for Molecular Medicine and Therapeutics, Department of Medical Genetics, BC Children's Hospital Research Institute, University of British Columbia, Vancouver, Canada.

Motivation: Deciphering the functional roles of cis-regulatory variants is a critical challenge in genome analysis and interpretation. It has been hypothesized that altered transcription factor (TF) binding events are a central mechanism by which cis-regulatory variants impact gene expression levels. However, we lack a computational framework to understand and quantify such mechanistic contributions. Read More

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December 2018

RNAi of Sterol Methyl Transferase1 Reveals its Direct Role in Diverting Intermediates Towards Withanolide/ Phytosterol Biosynthesis in Withania somnifera.

Plant Cell Physiol 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Aromatic Plants and Chiral Separation, Chemical Sciences Division, CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), India.

The medicinal properties of Ashwagandha (Withania somnifera) are accredited to a group of compounds called as withanolides. 24-methylene cholesterol is the intermediate for sterol biosynthesis and a proposed precursor of withanolides biogenesis. But, conversion of 24-methylene cholesterol to withaferin A and other withanolides has not yet been biochemically dissected. Read More

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December 2018

Agnostic Pathway/Gene Set Analysis of Genome-Wide Association Data Identifies Associations for Pancreatic Cancer.

Authors:
Naomi Walsh Han Zhang Paula L Hyland Qi Yang Evelina Mocci Mingfeng Zhang Erica J Childs Irene Collins Zhaoming Wang Alan A Arslan Laura Beane-Freeman Paige M Bracci Paul Brennan Federico Canzian Eric J Duell Steven Gallinger Graham G Giles Michael Goggins Gary E Goodman Phyllis J Goodman Rayjean J Hung Charles Kooperberg Robert C Kurtz Núria Malats Loic LeMarchand Rachel E Neale Sara H Olson Ghislaine Scelo Xiao O Shu Stephen K Van Den Eeden Kala Visvanathan Emily White Wei Zheng Demetrius Albanes Gabriella Andreotti Ana Babic William R Bamlet Sonja I Berndt Ayelet Borgida Marie-Christine Boutron-Ruault Lauren Brais Paul Brennan Bas Bueno-de-Mesquita Julie Buring Kari G Chaffee Stephen Chanock Sean Cleary Michelle Cotterchio Lenka Foretova Charles Fuchs J Michael M Gaziano Edward Giovannucci Michael Goggins Thilo Hackert Christopher Haiman Patricia Hartge Manal Hasan Kathy J Helzlsouer Joseph Herman Ivana Holcatova Elizabeth A Holly Robert Hoover Rayjean J Hung Vladimir Janout Eric A Klein Robert C Kurtz Daniel Laheru I-Min Lee Lingeng Lu Núria Malats Satu Mannisto Roger L Milne Ann L Oberg Irene Orlow Alpa V Patel Ulrike Peters Miquel Porta Francisco X Real Nathaniel Rothman Howard D Sesso Gianluca Severi Debra Silverman Oliver Strobel Malin Sund Mark D Thornquist Geoffrey S Tobias Jean Wactawski-Wende Nick Wareham Elisabete Weiderpass Nicolas Wentzensen William Wheeler Herbert Yu Anne Zeleniuch-Jacquotte Peter Kraft Donghui Li Eric J Jacobs Gloria M Petersen Brian M Wolpin Harvey A Risch Laufey T Amundadottir Kai Yu Alison P Klein Rachael Z Stolzenberg-Solomon

J Natl Cancer Inst 2018 Dec 12. Epub 2018 Dec 12.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD.

Background: Genome-wide association studies (GWAS) identify associations of individual single-nucleotide polymorphisms (SNPs) with cancer risk but usually only explain a fraction of the inherited variability. Pathway analysis of genetic variants is a powerful tool to identify networks of susceptibility genes.

Methods: We conducted a large agnostic pathway-based meta-analysis of GWAS data using the summary-based adaptive rank truncated product method to identify gene sets and pathways associated with pancreatic ductal adenocarcinoma (PDAC) in 9040 cases and 12 496 controls. Read More

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December 2018

Safety Considerations When Working with Humanized Animals.

ILAR J 2018 Dec 12. Epub 2018 Dec 12.

Unit for Laboratory Animal Medicine, University of Michigan Medical School in Ann Arbor, Michigan.

Research using laboratory animals has been revolutionized by the creation of humanized animal models, which are immunodeficient animals engrafted with human cells, tissues, or organs. These animal models provide the research community a unique and promising opportunity to mimic a wide variety of disease conditions in humans, from infectious disease to cancer. A vast majority of these models are humanized mice like those injected with human CD34+ hematopoietic stem cells and patient-derived xenografts. Read More

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December 2018

Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK.

Med Sci Monit 2018 Dec 12;24:9007-9018. Epub 2018 Dec 12.

Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China (mainland).

BACKGROUND Acyl-coenzymeA: cholesterol acyltransferase (ACAT) 1, a key enzyme converting excess free cholesterol to cholesterol esters, has been demonstrated to be associated with the pathogenesis of Alzheimer disease (AD). However, the mechanism underlying the protective role of ACAT1 blockage in AD progression remains elusive. MATERIAL AND METHODS Human neuroblastoma SH-SY5Y cells were treated for 24 h with increasing concentrations of aggregated Aβ₂₅₋₃₅ (5, 15, 25, and 45 μmol) with or without the ACAT1 siRNA pretreatment. Read More

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December 2018

1,25‑Dihydroxyvitamin D regulates macrophage polarization and ameliorates experimental inflammatory bowel disease by suppressing miR-125b.

Int Immunopharmacol 2018 Dec 9;67:106-118. Epub 2018 Dec 9.

Department of Immunology, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China; Key Laboratory of Tumor Prevention and Treatment of Heilongjiang Province, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China; Institute of Neural Tissue Engineering, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China. Electronic address:

Macrophages are highly plastic cells. Depending on stimulation, macrophages rapidly polarize to functionally distinct phenotypes that are involved in the pathogenesis of inflammatory bowel disease (IBD). 1,25‑Dihydroxyvitamin D (1,25(OH)D) has immunomodulatory activity, and 1,25(OH)D deficiency is correlated with autoimmune diseases, especially IBD. Read More

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December 2018

Gene network analysis of poplar root transcriptome in response to drought stress identifies a PtaJAZ3PtaRAP2.6-centered hierarchical network.

PLoS One 2018 12;13(12):e0208560. Epub 2018 Dec 12.

Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, United States of America.

Using time-series transcriptomic data from poplar roots undergoing polyethylene glycol (PEG)-induced drought stress, we built a genetic network model of the involved putative molecular responses. We found that the network resembled a hierarchical structure. The highest hierarchical level in this structure is occupied by 9 genes, which we called superhubs because they were primarily connected to 18 hub genes, which are then connected to 2,934 terminal genes. Read More

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December 2018

Coordinated regulation of transcription by CcpA and the Staphylococcus aureus two-component system HptRS.

PLoS One 2018 12;13(12):e0207161. Epub 2018 Dec 12.

School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.

The success of Staphylococcus aureus as a pathogen is due in part to its ability to adapt to changing environmental conditions using signal transduction pathways, such as metabolite- responsive regulators and two-component systems. S. aureus has a two-component system encoded by the gene pair sav0224 (hptS) and sav0223 (hptR) that regulate the hexose phosphate transport (uhpT) system in response to extracellular glucose-6-phosphate. Read More

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December 2018

Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.

PLoS Genet 2018 Dec 12;14(12):e1007702. Epub 2018 Dec 12.

Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russia.

Drosophila bithorax complex (BX-C) is one of the best model systems for studying the role of boundaries (insulators) in gene regulation. Expression of three homeotic genes, Ubx, abd-A, and Abd-B, is orchestrated by nine parasegment-specific regulatory domains. These domains are flanked by boundary elements, which function to block crosstalk between adjacent domains, ensuring that they can act autonomously. Read More

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December 2018

Fecal Microbiota Transplantation for Recurrent Clostridium difficile Infection and Other Conditions in Children: A Joint Position Paper From the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition and the European Society for Pediatric Gastroenterology, Hepatology, and Nutrition.

J Pediatr Gastroenterol Nutr 2018 Dec 11. Epub 2018 Dec 11.

Section of Pediatric Gastroenterology and Nutrition, Texas Children's Hospital, Baylor College of Medicine, Children's Nutrition and Research Center, Houston, TX.

Fecal microbiota transplantation (FMT) is becoming part of the treatment algorithms against recurrent Clostridium difficile infection (rCDI) both in adult and pediatric gastroenterology practice. With our increasing recognition of the critical role the microbiome plays in human health and disease, FMT is also being considered as a potential therapy for other disorders, including inflammatory bowel disease (Crohn disease, ulcerative colitis), graft versus host disease, neuropsychiatric diseases, and metabolic syndrome. Controlled trials with FMT for rCDI have not been performed in children, and numerous clinical and regulatory considerations have to be considered when using this untraditional therapy. Read More

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December 2018

Aurora kinase B inhibitor barasertib (AZD1152) inhibits glucose metabolism in gastric cancer cells.

Anticancer Drugs 2019 Jan;30(1):19-26

Cancer Institute.

Barasertib is a highly selective Aurora kinase B (AURKB) inhibitor and has been widely applied in a variety of cancer cells to investigate the regulatory function of AURKB. However, the effect of barasertib on glucose metabolism in gastric cancer (GC) remains illustrated. Here, barasertib was identified to effectively reduce glucose uptake and lactate production in GC cells in a dose-dependent and time-dependent manner. Read More

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January 2019
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PD-1 Polymorphisms Are Associated with Susceptibility of Acute Anterior Uveitis in Chinese Population.

DNA Cell Biol 2018 Dec 12. Epub 2018 Dec 12.

1 The Eye Hospital of Wenzhou Medical University, School of Ophthalmology and Optometry, Wenzhou Medical University , Wenzhou, Zhejiang, China .

Acute anterior uveitis (AAU) is an ordinary type of uveitis, which is an autoimmune disease produced by T cells. Programmed apoptosis protein 1 (PD-1) is a vital negative regulatory protein of immune tolerance. We detect the single nucleotide polymorphisms (SNPs) rs41386349, rs10204525, and rs2227982 of PD-1 to investigate the correlation between PD-1 polymorphisms and AAU. Read More

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December 2018

Designing spatially distributed gene regulatory networks to elicit contrasting patterns.

ACS Synth Biol 2018 Dec 12. Epub 2018 Dec 12.

Pattern formation and differential interactions are important for microbial consortia to divide labor and perform complex functions. To obtain further insight into such interactions, we present a computational method for simulating physically separated microbial colonies each implementing different gene regulatory networks. We validate our theory by experimentally demonstrating control over gene expression patterns in a diffusion-mediated lateral inhibition circuit. Read More

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December 2018

Real-time Mobile Monitoring of the Dynamic Associations Among Motor Activity, Energy, Mood, and Sleep in Adults With Bipolar Disorder.

JAMA Psychiatry 2018 Dec 12. Epub 2018 Dec 12.

Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Importance: Biologic systems involved in the regulation of motor activity are intricately linked with other homeostatic systems such as sleep, feeding behavior, energy, and mood. Mobile monitoring technology (eg, actigraphy and ecological momentary assessment devices) allows the assessment of these multiple systems in real time. However, most clinical studies of mental disorders that use mobile devices have not focused on the dynamic associations between these systems. Read More

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December 2018

Conclusions of the digital health hub of the Transform Africa Summit (2018): strong government leadership and public-private-partnerships are key prerequisites for sustainable scale up of digital health in Africa.

BMC Proc 2018 15;12(Suppl 11):17. Epub 2018 Aug 15.

WHO Country Office, Kigali, Rwanda.

Background: The use of digital technologies to improve access to health is gaining momentum in Africa. This is more pertinent with the increasing penetration of mobile phone technology and internet use, and calls for innovative strategies to support implementation of the health-related Sustainable Development Goals and Universal Health Coverage on the continent. However, the huge potential benefits of digital health to advance health services delivery in Africa is yet to be fully harnessed due to critical challenges such as proliferation of pilot projects, poor coordination, inadequate preparedness of the African health workforce for digital health, lack of interoperability and inadequate sustainable financing, among others. Read More

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Health risk of swimming pool disinfection by-products: a regulatory perspective.

J Water Health 2018 Dec;16(6):947-957

Department of Microbiology, Eötvös Loránd University of Sciences, Pázmány Péter sétány 1/C, H-1117, Hungary.

While disinfection of swimming pools is indispensable for microbiological safety, it may lead to the formation of disinfection by-products. Most studies agree that inhalation exposure is the predominant pathway of the associated health risks, but assumptions are based on concentrations measured in water and evaporation models. Pool water and air were sampled in 19 swimming pools. Read More

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December 2018

Body image satisfaction and subjective wellbeing among ninth-grade students attending state schools in Canoas, Brazil.

Cien Saude Colet 2018 Dec;23(12):4289-4298

Programa de Pós-Graduação em Saúde Coletiva , Universidade Luterana do Brasil . R. São Luis 101, Centro. 92420-280 Canoas RS Brasil .

During adolescence, body image is linked to subjective well-being. This study aimed to identify variables related to overall well-being (life satisfaction, health perception, happiness, and optimism) that discriminate between teenagers who are satisfied with body image and those who are dissatisfied. A cross-sectional, school- based study was conducted involving a sample of 1,460 ninth-grade students attending state schools in Canoas in the State of Rio Grande do Sul, Brazil. Read More

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December 2018

Health and Food and Nutritional Security Policies: challenges in controlling childhood obesity.

Cien Saude Colet 2018 Dec;23(12):4143-4152

Departamento de Nutrição Social, Faculdade de Nutrição, Universidade Federal Fluminense. R. Mario Santos Braga 30/4º, Centro. 24020-140 Niterói RJ Brasil.

The study analyzed the initiatives of prevention and control of childhood obesity, especially those of Adequate and Healthy Food Promotion (PAAS) which have been part of the policies of the Brazilian federal government for the last 15 years. All documents that feature PAAS initiatives in the food and nutrition security, as well as public health policy fields, were evaluated according to the following criteria: (1) the approach to PAAS initiatives; (2) the aspects of obesitythat they intend to affect and (3) potential interest disputes. The main PAAS initiatives identified are intended to encourage: food and nutrition education; agroecological production systems; family agriculture; food accessibility; healthy environments and regulatory measures. Read More

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December 2018
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Macrophage Raptor Deficiency-Induced Lysosome Dysfunction Exacerbates Nonalcoholic Steatohepatitis.

Cell Mol Gastroenterol Hepatol 2018 Sep 19;7(1):211-231. Epub 2018 Sep 19.

Tianjin Key Laboratory of Metabolic Diseases, Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, China. Electronic address:

Background & Aims: Nonalcoholic steatohepatitis (NASH) is an increasingly prevalent nonalcoholic fatty liver disease, characterized by inflammatory cell infiltration and hepatocellular damage. Mammalian target of rapamycin complex 1 (mTORC1) has been investigated extensively in the context of cancer, including hepatocellular carcinoma. However, the role of mTORC1 in NASH remains largely unknown. Read More

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September 2018

Elevated regulatory T cells at diagnosis of Coccidioides infection associates with chronicity in pediatric patients.

J Allergy Clin Immunol 2018 Dec 26;142(6):1971-1974.e7. Epub 2018 Oct 26.

Department of Molecular and Cell Biology, School of Natural Sciences, University of California Merced, Merced, Calif; Health Sciences Research Institute, University of California Merced, Merced, Calif. Electronic address:

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December 2018

The tolerant immune system: Biological significance and clinical implications of T cell tolerance.

Endocr Metab Immune Disord Drug Targets 2018 Dec 11. Epub 2018 Dec 11.

Department of Basic Medical Sciences, Neuroscience and Sensory Organs, School of Medicine, University of Bari, Bari. Italy.

Background: T cell tolerance both at thymic and peripheral levels is a mechanism of protection finalized to eradicate autoreactive T cell clones and/or to maintain immune homeostasis, especially, postnatally. Central tolerance occurs in the thymic medulla via a mechanism of negative selection which leads to the eradication of autoreactive T cell clones. Mechanisms of action: Such a tolerogenic event relies on Fas-mediated apoptosis of autoreactive T cell clones operated by thymic dendritic cells (DCs), on the one hand. Read More

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December 2018
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Generation of a primary hyperoxaluria type 1 disease model via CRISPR/Cas9 system in rats.

Curr Mol Med 2018 Dec 11. Epub 2018 Dec 11.

Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai. China.

Background: Primary hyperoxaluria type 1 (PH1) is an inherited disease caused by mutations in alanine-glyoxylate aminotransferase (AGXT). It is characterized by abnormal metabolism of glyoxylic acid in liver leading to endogenous oxalate overproduction and deposition of oxalate in multiple organs, mainly the kidney. Patients of PH1 often suffer from recurrent urinary tract stones, and finally renal failure. Read More

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December 2018
2 Reads

Quorum quenching: role of nanoparticles as signal jammers in Gram-negative bacteria.

Future Microbiol 2018 Dec 12. Epub 2018 Dec 12.

Department of Microbiology, Government College University, Faisalabad, Pakistan.

Quorum sensing (QS) is a cell density dependent regulatory process that uses signaling molecules to manage the expression of virulence genes and biofilm formation. The study of QS inhibitors has emerged as one of the most fascinating areas of research to discover novel antimicrobial agents. Compounds that block QS have become candidates as unusual antimicrobial agents, as they are leading players in the regulation of virulence of drug-resistant pathogens. Read More

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December 2018
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Anti-factor H Autoantibodies Assay by ELISA.

Methods Mol Biol 2019 ;1901:191-196

Laboratoire d'Immunologie, Hôpital Européen Georges Pompidou, Paris, France.

The complement system is a part of the immune system implicated in host defense against pathogens and damaged cells and Factor H is the main regulatory protein of this powerful enzymatic cascade. Autoantibodies directed against Factor H (anti-FH antibodies) are implicated in different pathologies mainly atypical hemolytic and uremic syndrome and C3 glomerulopathies. The detection and quantification of these autoantibodies are crucial for the clinical management of the patients. Read More

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January 2019

RNA Modification Regulatory Genes in DNA Damage.

Methods Mol Biol 2019 ;1870:249-262

Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.

Expression of genetic information is a multistep process which needs to be tightly regulated. One of the regulatory mechanisms is posttranscriptional modification of RNA, which can alter the stability, expression, or protein composition. Therefore, misregulation of this important cellular process can lead to pathological consequences, such as cancer development. Read More

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January 2019

Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE.

Methods Mol Biol 2019 ;1870:199-208

Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany.

Alternative splicing allows genes to express isoforms with different coding or regulatory functions on demand. While short read deep sequencing technologies (RNA-seq) provide an immediate measurement of local splicing events, the phasing of these events along full-length isoforms requires the computational inference of long-range dependencies from short-range data points. We introduce CIDANE, a tool for the assembly and quantification of full-length isoforms from short read RNA-seq data. Read More

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January 2019

Visualization of Xist Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System.

Methods Mol Biol 2019 ;1870:41-50

Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA.

X-inactive specific transcript (Xist) is a long noncoding RNA that is essential for initiating and maintaining epigenetic silencing of one copy of the X chromosome in mammalian females. But the mechanism by which Xist localizes and spreads on the X chromosome and facilitates transcriptional silencing remains largely unknown. This limited understanding, at least in part, is due to the technical difficulties in the visualization and functional characterization of Xist. Read More

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January 2019

Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies.

Methods Mol Biol 2019 ;1904:83-108

Department of Clinical Pharmacology, Genentech Research and Early Development, South San Francisco, CA, USA.

Metastatic melanoma is a dreadful type of skin cancer arising due to uncontrolled proliferation of melanocytes. It has very poor prognosis, low 5-year survival rates and until recently there were only handful of treatment options for metastatic melanoma patients. The drugs that were approved for the treatment had low response rates and were associated with severe adverse events. Read More

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January 2019
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Regulatory T cells regulate the distribution of natural killer T cells through CD39 signal transduction in asthma.

Hum Cell 2018 Dec 11. Epub 2018 Dec 11.

Department of Pediatrics, South Campus, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, No. 2000 Jiangyue Road, Minhang District, Shanghai, 201112, People's Republic of China.

Natural killer T cells (NKT cells) and regulatory T cells (Treg cells) are two important immune regulatory cells which both play critical roles in asthma. Our previous experiments revealed that activation of Treg cells suppressed NKT cells in asthma. However, the possible regulatory effects and the mechanisms linking Treg cells and NKT cells remain poorly understood. Read More

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December 2018

FOXG1 Regulates PRKAR2B Transcriptionally and Posttranscriptionally via miR200 in the Adult Hippocampus.

Mol Neurobiol 2018 Dec 11. Epub 2018 Dec 11.

Institute of Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine, Albert-Ludwigs-University Freiburg, 79104, Freiburg, Germany.

Rett syndrome is a complex neurodevelopmental disorder that is mainly caused by mutations in MECP2. However, mutations in FOXG1 cause a less frequent form of atypical Rett syndrome, called FOXG1 syndrome. FOXG1 is a key transcription factor crucial for forebrain development, where it maintains the balance between progenitor proliferation and neuronal differentiation. Read More

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December 2018

Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel.

Appl Microbiol Biotechnol 2018 Dec 11. Epub 2018 Dec 11.

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.

The environmental strain Aeromonas salmonicida subsp. pectinolytica 34mel produces abundant melanin through the homogentisate pathway in several culture media, but unexpectedly not when grown in a medium containing glycerol. Using this observation as a starting point, this study investigated the underlying causes of the inhibition of melanin synthesis by glycerol, to shed light on factors that affect melanin production in this microorganism. Read More

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December 2018
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Evaluating Potential for Using Municipal Sewage Sludge in the Rehabilitation of Ground Degraded by the Sodium Processing Industry.

Bull Environ Contam Toxicol 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Geology, Geophysics and Environmental Protection, Department of Environmental Protection, AGH University of Science and Technology, Mickiewicza 30 av, 30-059, Krakow, Poland.

The paper attempts at evaluating potential for the use of sewage sludge produced in a rural area for the rehabilitation of ground degraded by the sodium processing industry. The results demonstrated that the studied sewage sludge conforms to the current regulatory standards, enabling its use in land rehabilitation, both for agricultural and non-agricultural purposes. Ground in areas degraded by the KZS "Solvay" industrial plant has poor parameters in terms of pH, and moderate parameters in terms of humic substance content. Read More

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December 2018

Role of viruses, prions and miRNA in neurodegenerative disorders and dementia.

Virusdisease 2018 Dec 29;29(4):419-433. Epub 2018 Sep 29.

Drug Design & Development Section, Translational Gerontology Branch, Intramural Research Program, National, Institute on Aging, National Institutes of Health, Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224 USA.

Dementia is known as loss of cellular communications in the brain at a region caused by multi-factorial diseases and pathogenic infections. Approximately eighty percent reported cases of Alzheimer's disease are followed by vascular dementia. The common symptoms of dementia include memory loss, concentration problems, thinking, and language solving situations. Read More

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December 2018

IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response.

J Immunol Res 2018 13;2018:1827901. Epub 2018 Nov 13.

Life Science School, Liaoning University, Shenyang 110036, China.

Background: Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response in colon lamina propria and IL-33 induced both regulatory B cells (Bregs) and regulatory T cells (Tregs) in mesenteric lymph nodes (MLNs) of mice with DSS-induced acute colitis. Moreover, we speculated that IL-33 would promote the Treg or Breg responses leading to the attenuation of DSS-induced chronic colitis. So, we investigated the role of IL-33 on Bregs and Tregs in the MLN of DSS-induced chronic colitis mice. Read More

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November 2018

Brain of the blind: transcriptomics of the golden-line cavefish brain.

Curr Zool 2018 Dec 12;64(6):765-773. Epub 2018 Jan 12.

Institute of Neuroscience, University of Oregon, Eugene, OR, USA.

The genus (golden-line barbel) includes 25 species of cave-dwelling blind fish (cavefish) and more than 30 surface-dwelling species with normal vision. Cave environments are dark and generally nutrient-poor with few predators. Cavefish of several genera evolved convergent morphological adaptations in visual, pigmentation, brain, olfactory, and digestive systems. Read More

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December 2018
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A Genome-Wide Study of Allele-Specific Expression in Colorectal Cancer.

Front Genet 2018 27;9:570. Epub 2018 Nov 27.

Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Accumulating evidence from small-scale studies has suggested that allele-specific expression (ASE) plays an important role in tumor initiation and progression. However, little is known about genome-wide ASE in tumors. In this study, we conducted a comprehensive analysis of ASE in individuals with colorectal cancer (CRC) on a genome-wide scale. Read More

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November 2018

Revisiting the Concept of Targeting NFAT to Control T Cell Immunity and Autoimmune Diseases.

Front Immunol 2018 27;9:2747. Epub 2018 Nov 27.

Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, South Korea.

The nuclear factor of activated T cells (NFAT) family of transcription factors, which includes NFAT1, NFAT2, and NFAT4, are well-known to play important roles in T cell activation. Most of NFAT proteins are controlled by calcium influx upon T cell receptor and costimulatory signaling results increase of IL-2 and IL-2 receptor. NFAT3 however is not shown to be expressed in T cells and NFAT5 has not much highlighted in T cell functions yet. Read More

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November 2018

Functional Microdomains in Heart's Pacemaker: A Step Beyond Classical Electrophysiology and Remodeling.

Front Physiol 2018 27;9:1686. Epub 2018 Nov 27.

Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

Spontaneous beating of the sinoatrial node (SAN), the primary pacemaker of the heart, is initiated, sustained, and regulated by a complex system that integrates ion channels and transporters on the cell membrane surface (often referred to as "membrane clock") with subcellular calcium handling machinery (by parity of reasoning referred to as an intracellular "Ca clock"). Stable, rhythmic beating of the SAN is ensured by a rigorous synchronization between these two clocks highlighted in the coupled-clock system concept of SAN timekeeping. The emerging results demonstrate that such synchronization of the complex pacemaking machinery at the cellular level depends on tightly regulated spatiotemporal signals which are restricted to precise sub-cellular microdomains and associated with discrete clusters of different ion channels, transporters, and regulatory receptors. Read More

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November 2018

In vitro pharmacological characterization of SPN-810M (molindone).

J Exp Pharmacol 2018 21;10:65-73. Epub 2018 Nov 21.

Preclinical Toxicology, Supernus Pharmaceuticals, Inc., Rockville, MD, USA.

Background: Impulsive aggression (IA) is considered a maladaptive form of aggression that is reactive and overt and occurs outside of the acceptable social context. Many children and adolescents with attention-deficit/hyperactivity disorder (ADHD) display clinically significant aggression, with the predominant subtype being IA. However, there is currently no Food and Drug Administration-approved medication specifically to treat IA. Read More

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November 2018
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A methodological review of national and transnational pharmaceutical budget impact analysis guidelines for new drug submissions.

Clinicoecon Outcomes Res 2018 26;10:821-854. Epub 2018 Nov 26.

Department of Health Research Methods, Evidence and Impact (HEI), McMaster University, Hamilton, ON, Canada,

Introduction: Budget impact analysis (BIA) in health care, sometimes referred to as resource impact, is the financial change in the use of health resources associated with adding a new drug to a formulary or the adoption of a new health technology. Several national and transnational organizations worldwide have updated their BIA guidelines in the past 4 years. The aim of the present review was to provide a comprehensive list of the key recommendations of BIA guidelines from different countries that may be of interest for those who wish to build or to update BIA guidelines. Read More

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November 2018

Exosome-mediated delivery of functionally active miRNA-142-3p inhibitor reduces tumorigenicity of breast cancer in vitro and in vivo.

Int J Nanomedicine 2018 20;13:7727-7747. Epub 2018 Nov 20.

Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran,

Background: Exosomes, widely recognized natural nanovesicles, represent one of the recently discovered modes of intercellular communication due to their ability to transmit crucial cellular information that can be engineered to have robust delivery and targeting capacity. MiR-142-3p, one of the upregulated microRNAs (miRNAs) in many types of breast cancer, activates the canonical Wnt signaling pathway and transactivates the miR-150 expression, and results in the hyperproliferation of cancer cells in vitro and mammary glands in vivo.

Materials And Methods: In this study, we exploited the exosomes isolated from bone marrow-derived mesenchymal stem cells (MSCs-Exo) to deliver LNA (locked nucleic acid)-modified anti-miR-142-3p oligonucleotides to suppress the expression level of miR-142-3p and miR-150 in 4T1 and TUBO breast cancer cell lines. Read More

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November 2018

Role of miRNAs in skeletal muscle aging.

Clin Interv Aging 2018 22;13:2407-2419. Epub 2018 Nov 22.

Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin, China,

Purpose: The aim of this study was to explore the role of miRNAs in the process of skeletal muscle aging.

Materials And Methods: We analyzed the miRNA microarray datasets from 19 young and 17 old skeletal muscle samples by bioinformatic analysis. Differentially expressed miRNAs were identified, followed by function and pathway enrichment analysis. Read More

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November 2018
1.824 Impact Factor

Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis.

Oncogene 2018 Dec 11. Epub 2018 Dec 11.

Trinity Biomedical Sciences Institute, School of Biochemistry & Immunology, Trinity College Dublin, Dublin 2, Ireland.

Murine inflammatory caspase-11 has an important role in intestinal epithelial inflammation and barrier function. Activation of the non-canonical inflammasome, mediated by caspase-11, serves as a regulatory pathway for the production of the pro-inflammatory cytokines IL-1β and IL-18, and has a key role in pyroptotic cell death. We have previously demonstrated a protective role for caspase-11 during dextran sulphate sodium (DSS)-induced colitis, however the importance of caspase-11 during colorectal tumour development remains unclear. Read More

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December 2018

Progesterone receptor integrates the effects of mutated MED12 and altered DNA methylation to stimulate RANKL expression and stem cell proliferation in uterine leiomyoma.

Oncogene 2018 Dec 11. Epub 2018 Dec 11.

Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.

Progesterone and its receptor, PR, are essential for uterine leiomyoma (LM, a.k.a. Read More

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December 2018

By reducing global mRNA translation in several ways, 2-deoxyglucose lowers MCL-1 protein and sensitizes hemopoietic tumor cells to BH3 mimetic ABT737.

Cell Death Differ 2018 Dec 11. Epub 2018 Dec 11.

The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.

Drugs targeting various pro-survival BCL-2 family members (''BH3 mimetics'') have efficacy in hemopoietic malignancies, but the non-targeted pro-survival family members can promote resistance. Pertinently, the sensitivity of some tumor cell lines to BH3 mimetic ABT737, which targets BCL-2, BCL-XL, and BCL-W but not MCL-1, is enhanced by 2-deoxyglucose (2DG). We found that 2DG augmented apoptosis induced by ABT737 in 3 of 8 human hemopoietic tumor cell lines, most strongly in pre-B acute lymphocytic leukemia cell line NALM-6, the focus of our mechanistic studies. Read More

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December 2018
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CCR2 enhances CD25 expression by FoxP3 regulatory T cells and regulates their abundance independently of chemotaxis and CCR2 myeloid cells.

Cell Mol Immunol 2018 Dec 11. Epub 2018 Dec 11.

The Walter & Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.

A wide array of chemokine receptors, including CCR2, are known to control Treg migration. Here, we report that CCR2 regulates Tregs beyond chemotaxis. We found that CCR2 deficiency reduced CD25 expression by FoxP3 Treg cells. Read More

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December 2018

CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages.

Nat Commun 2018 12 11;9(1):5279. Epub 2018 Dec 11.

Regeneration in Hematopoiesis and Animal Models in Hematopoiesis, Institute for Immunology, Fetscherstr. 74, 01307, Dresden, Germany.

Regulatory mechanisms controlling the pool size of spleen dendritic cells (DC) remain incompletely understood. DCs are continuously replenished from hematopoietic stem cells, and FLT3-mediated signals cell-intrinsically regulate homeostatic expansion of spleen DCs. Here we show that combining FLT3 and CSF1R-deficiencies results in specific and complete abrogation of spleen DCs in vivo. Read More

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December 2018

Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants.

Nat Commun 2018 12 11;9(1):5283. Epub 2018 Dec 11.

Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8501, Japan.

Evolutionary mechanisms underlying innovation of cell types have remained largely unclear. In multicellular eukaryotes, the evolutionary molecular origin of sperm differentiation is unknown in most lineages. Here, we report that in algal ancestors of land plants, changes in the DNA-binding domain of the ancestor of the MYB transcription factor DUO1 enabled the recognition of a new cis-regulatory element. Read More

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December 2018
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