72,856 results match your criteria Regenerative Medicine [Journal]


Novel endotypes in heart failure: effects on guideline-directed medical therapy.

Eur Heart J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cardiology, University of Groningen, Hanzeplein 1, Groningen, the Netherlands.

Aims: We sought to determine subtypes of patients with heart failure (HF) with a distinct clinical profile and treatment response, using a wide range of biomarkers from various pathophysiological domains.

Methods And Results: We performed unsupervised cluster analysis using 92 established cardiovascular biomarkers to identify mutually exclusive subgroups (endotypes) of 1802 patients with HF and reduced ejection fraction (HFrEF) from the BIOSTAT-CHF project. We validated our findings in an independent cohort of 813 patients. Read More

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December 2018

CRISPR-C: circularization of genes and chromosome by CRISPR in human cells.

Nucleic Acids Res 2018 Dec;46(22):e131

Department of Biomedicine, Aarhus University, Denmark.

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. Read More

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December 2018

The Generation of Hematopoietic Progenitor Cells from Endothelial Cells by Silymarin.

Clin Lab 2018 Oct;64(11)

Background: The generation of hematopoietic cells from endothelial cells may determine a novel approach for the production of blood cells. Regenerative functions of Silymarin, a mixture of polyphenolic flavonoids derived from milk thistle, have previously been reported. The purpose of this study was to investigate the direct effect of Silymarin on conversion of endothelial cells to hematopoietic cells. Read More

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October 2018

Macromolecular crowding as a means to assess the effectiveness of chondrogenic media.

J Tissue Eng Regen Med 2018 Dec 14. Epub 2018 Dec 14.

Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.

Chondrocyte-based tissue engineering requires in vitro cell expansion, which is associated with phenotypic losses, decrease in collagen II synthesis and increase in collagen I synthesis. Another major obstacle in clinical translation of chondrocyte-based therapies is the lack of extracellular matrix (ECM) in the engineered cartilage substitutes. Various research and commercially available media claim that can maintain chondrogenic phenotype, whilst macromolecular crowding (MMC) has been shown to increase tissue-specific ECM deposition and to maintain cell phenotype in vitro. Read More

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December 2018

DNA damage signatures in peripheral blood cells (PBMC) as biomarkers in prodromal Huntington's disease.

Ann Neurol 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, University of Naples "Federico II", Naples, Italy.

Easily accessible biomarkers in Huntington disease (HD) are actively searched. We investigated telomere length (TL) and DNA double-strand breaks (histone variant pγ-H2AX) as predictive disease biomarkers in peripheral blood mononuclear cells (PBMC) from 25 pre-manifest, 58 HD patients, with similar CAG expansion in the huntingtin gene (HTT) gene and 44 healthy controls (HC). PBMC from PRE-HD and HD groups showed shorter telomeres (p<0. Read More

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December 2018

Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes.

Hepatology 2018 Dec 14. Epub 2018 Dec 14.

School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo biological screens. Induced pluripotent stem cells (iPSC) may help to circumvent this obstacle, but often harbor genetic and epigenetic abnormalities limiting their potential. Here, we describe the pharmacological induction of proliferative human hepatic progenitor cells (HPC) through a cocktail of growth factors and small molecules mimicking the signaling events involved in liver regeneration. Read More

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December 2018

Minimal residual disease and log-reduction of plasma cells are associated with superior response after double autologous stem cell transplant in younger patients with multiple myeloma.

Cytometry B Clin Cytom 2018 Dec 13. Epub 2018 Dec 13.

Department of Hematology and Stem Cell Transplant Unit, Fondazione IRCCS "Casa Sollievo della Sofferenza", San Giovanni Rotondo, Italy.

Background: Optimization of chemotherapy regimens in the treatment of multiple myeloma (MM) has led to increase the frequency of cases with complete response (CR). Nonetheless, many MM patients still experience relapse, suggesting that CR represents a suboptimal response criteria, and that new therapeutic strategies are needed after single transplant. However, the role of double autologous stem cell transplant (ASCT) as new adjunctive strategy remains to be elucidated. Read More

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December 2018

Biomaterial-induced multinucleated giant cells express proinflammatory signaling molecules: A histological study in humans.

J Biomed Mater Res A 2018 Dec 13. Epub 2018 Dec 13.

Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, FORM (Frankfurt Orofacial Regenerative Medicine) Lab, University Hospital Frankfurt Goethe University, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany.

The biomaterials physicochemical characteristics influence their cellular reaction, degradation and regenerative capacities. Macrophages and multinucleated giant cells (MNGCs) are observed in the augmentation area of biomaterials. This study, for the first time, evaluated the polarization pattern of macrophages and MNGCs in response to two different bone substitute materials (synthetic bone substitute material (SBSM) = NanoBone® vs. Read More

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December 2018
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Toward zonally tailored scaffolds for osteochondral differentiation of synovial mesenchymal stem cells.

J Biomed Mater Res B Appl Biomater 2018 Dec 13. Epub 2018 Dec 13.

Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York.

Synovium-derived mesenchymal stem cells (SMSCs) are an emerging cell source for regenerative medicine applications, including osteochondral defect (OCD) repair. However, in contrast to bone marrow MSCs, scaffold compositions which promote SMSC chondrogenesis/osteogenesis are still being identified. In the present manuscript, we examine poly(ethylene) glycol (PEG)-based scaffolds containing zonally-specific biochemical cues to guide SMSC osteochondral differentiation. Read More

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December 2018

Insulin-Like Growth Factor I Regulation and Its Actions in Skeletal Muscle.

Compr Physiol 2018 Dec 13;9(1):413-438. Epub 2018 Dec 13.

Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, Florida, USA.

The insulin-like growth factor (IGF) pathway is essential for promoting growth and survival of virtually all tissues. It bears high homology to its related protein insulin, and as such, there is an interplay between these molecules with regard to their anabolic and metabolic functions. Skeletal muscle produces a significant proportion of IGF-1, and is highly responsive to its actions, including increased muscle mass and improved regenerative capacity. Read More

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December 2018
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FLiRT Technique "Fistula Lipocytes Regenerative Therapy"; autologous adipose derived mesenchymal stem cells auto-transplantation to treat complex fistula-in-ano - Video Vignette.

Colorectal Dis 2018 Dec 13. Epub 2018 Dec 13.

Department of General Surgery UOC C, University of Rome Tor Vergata, Rome, Italy.

Various surgical options have been proposed for the treatment of Fistula-in-Ano (FIA). A balance between healing, recurrence, and continence preservation rates should be maintained which appears to be difficult in complex FIA, such as recurrent, high trans-sphincteric, anterior fistula in a female, multiple tracts, and patients with Crohn's disease. This article is protected by copyright. Read More

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December 2018

Isolation and culture of human primary keratinocytes - a methods review.

Exp Dermatol 2018 Dec 13. Epub 2018 Dec 13.

Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.

Keratinocyte culture is a necessary and widely used tool in a variety of experimental dermatological and biomedical studies. Literature search has revealed a variety of different protocols of human primary keratinocyte isolation and culture. Therefore, the aim of this paper was to review and summarize current trends in human primary keratinocyte culture techniques. Read More

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December 2018

Cross-talk of healthy and impaired human tissues for dissection of disease pathogenesis.

Biotechnol Prog 2018 Dec 13:e2766. Epub 2018 Dec 13.

Venetian Institute of Molecular Medicine, Via Orus 2, 35131, Padova, Italy.

Systemic diseases affect multiple tissues that interact with each other within a network difficult to explore at the body level. However, understanding the inter-dependences between tissues could be of high relevance for drug target identification, especially at the first stages of disease development. In vitro systems have the advantages of accessibility to measurements and precise controllability of culture conditions, but currently have limitations in mimicking human in vivo systemic tissue response. Read More

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December 2018

A New Era of Cardiac Cell Therapy: Opportunities and Challenges.

Adv Healthc Mater 2018 Dec 13:e1801011. Epub 2018 Dec 13.

Department of Molecular Biomedical Sciences and Comparative Medicine Institute, North Carolina State University, Raleigh, NC, 27607, USA.

Myocardial infarction (MI), caused by coronary heart disease (CHD), remains one of the most common causes of death in the United States. Over the last few decades, scientists have invested considerable resources on the study and development of cell therapies for myocardial regeneration after MI. However, due to a number of limitations, they are not yet readily available for clinical applications. Read More

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December 2018

Fabrication of Kidney Proximal Tubule Grafts Using Biofunctionalized Electrospun Polymer Scaffolds.

Macromol Biosci 2018 Dec 13:e1800412. Epub 2018 Dec 13.

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Universiteitsweg 99,, 3584, CG Utrecht, The Netherlands.

The increasing prevalence of end-stage renal disease and persistent shortage of donor organs call for alternative therapies for kidney patients. Dialysis remains an inferior treatment as clearance of large and protein-bound waste products depends on active tubular secretion. Biofabricated tissues could make a valuable contribution, but kidneys are highly intricate and multifunctional organs. Read More

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December 2018

Altered frontal aslant tracts as a heritable neural basis of social communication deficits in autism spectrum disorder: A sibling study using tract-based automatic analysis.

Autism Res 2018 Dec 12. Epub 2018 Dec 12.

Institute of Medical Device and Imaging, National Taiwan University College of Medicine, Taipei, Taiwan.

Investigating social behaviors and brain structural alterations in unaffected siblings of individuals with autism spectrum disorder (ASD) may help identify intermediate phenotypes of social communication deficits in ASD. This study hypothesized that such intermediate phenotypes could be identified in white matter tracts of the social communication model that exhibited reduced tract integrity and associations with social communication deficits. Boys with ASD (N = 30), unaffected male siblings (N = 27), and typically developing (TD) boys (N = 30) underwent clinical evaluation and MRI scanning. Read More

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December 2018

Necessity for standardization of relative centrifugal force values in studies on platelet rich fibrin; response to letter to the editor.

J Periodontol 2018 Dec 13. Epub 2018 Dec 13.

FORM, Frankfurt Oral Regenerative Medicine, Clinic for Maxillofacial and Plastic Surgery, Johann Wolfgang Goethe University, Frankfurt Am Main, Germany.

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December 2018

Radial Glia in Echinoderms.

Dev Neurobiol 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology, University of North Florida, Jacksonville, Florida.

Radial glial cells are crucial in vertebrate neural development and regeneration. It has been recently proposed that this neurogenic cell type might be older than the chordate lineage itself and might have been present in the last common deuterostome ancestor. Here, we summarize the results of recent studies on radial glia in echinoderms, a highly regenerative phylum of marine invertebrates with shared ancestry to chordates. Read More

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December 2018

Generation of Pancreatic Ductal Organoids and Whole-Mount Immunostaining of Intact Organoids.

Curr Protoc Cell Biol 2018 Dec 12:e82. Epub 2018 Dec 12.

Section of Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.

Traditionally, studies of cells and tissues have been performed on isolated primary cells or immortalized cell lines by culturing them in 2D culture dishes or flasks. However, a caveat regarding 2D culture is that the cells poorly recapitulate their in vivo counterparts, mainly due to a lack of 3D cell-cell and cell-extracellular matrix interactions. In recent years, the development of in vitro organoids as 3D culture has gained substantial attention as a model to study different tissues. Read More

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December 2018

Clinical implementation of gene panel testing for lysosomal storage diseases.

Mol Genet Genomic Med 2018 Dec 11. Epub 2018 Dec 11.

Paediatric Neurology Unit, Department of Paediatrics, UZ Brussel, Brussels, Belgium.

Background: The diagnostic workup in patients with a clinical suspicion of lysosomal storage diseases (LSD) is often difficult due to the variability in the clinical phenotype. The gold standard for diagnosis of LSDs consists of enzymatic testing. However, due to the sequential nature of this methodology and inconsistent genotype-phenotype correlations of certain LSDs, finding a diagnosis can be challenging. Read More

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December 2018

miR-223 promotes regenerative myeloid cell phenotype and function in the demyelinated central nervous system.

Glia 2018 Dec 11. Epub 2018 Dec 11.

Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland, Canada.

In the injured central nervous system, myeloid cells, including macrophages and microglia, are key contributors to both myelin injury and repair. This immense plasticity emphasizes the need to further understand the precise molecular mechanisms that contribute to the dynamic regulation of myeloid cell polarization and function. Herein, we demonstrate that miR-223 is upregulated in multiple sclerosis (MS) patient monocytes and the alternatively-activated and tissue-regenerating M2-polarized human macrophages and microglia. Read More

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December 2018

PACAP through EGFR transactivation preserves human corneal endothelial integrity.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Department of Biomedical and Biotechnological Sciences, Section of Human Anatomy and Histology, University of Catania, Catania, Italy.

The corneal endothelium is composed of a single hexagonal-shaped cells layer adherent to the Descemet's membrane. The primary function of these cells is maintaining of tissue clarity by regulating its hydration. Trauma, aging or other pathologies cause their loss, counterbalanced by enlargement of survived cells unable to guarantee an efficient fluid pumping to and from the stroma. Read More

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December 2018
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Breast cancer-linked lncRNA u-Eleanor is upregulated in breast of healthy women with lack or short duration of breastfeeding.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.

Recently, it has been revealed that estrogen-related reproductive factors are linked with some early gene expression lesions associated with malignancy in clinically healthy breasts. Accordingly, the aim of the current study was to evaluate the association of expression levels of estrogen-related long noncoding RNAs (lncRNAs) upstream Eleanor (u-Eleanor) and HOX antisense intergenic RNA (HOTAIR) with the different patterns of reproductive factors in breast tissue of healthy women. The subjects of this study were 98 cancer-free women who had undergone cosmetic mammoplasty. Read More

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December 2018

Mesenchymal stem cells facilitate cardiac differentiation in Sox2-expressing cardiac C-kit cells in coculture.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang, Malaysia.

Stem cell therapy offers hope to reconstitute injured myocardium and salvage heart from failing. A recent approach using combinations of derived Cardiac-derived c-kit expressing cells (CCs) and mesenchymal stem cells (MSCs) in transplantation improved infarcted hearts with a greater functional outcome, but the effects of MSCs on CCs remain to be elucidated. We used a novel two-step protocol to clonogenically amplify colony forming c-kit expressing cells from 4- to 6-week-old C57BL/6N mice. Read More

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December 2018

Folding mutations suppress early beta-cell proliferation.

Elife 2018 Dec 14;7. Epub 2018 Dec 14.

Diabetes Research Group, Life Sciences Institute, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, Canada.

Exploring how proliferation and maturation of beta-cells can be impaired after birth will shed light on the origins of various forms of diabetes. Read More

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December 2018

Mapping theme trends and knowledge structure on adipose-derived stem cells: a bibliometric analysis from 2003 to 2017.

Regen Med 2018 Dec 14. Epub 2018 Dec 14.

Department of Physiology, College of Life Science, China Medical University, Shenyang 110122, PR China.

Aim: To investigate the theme trends and knowledge structure of adipose-derived stem cells (ADSCs) related literatures by using bibliometric analysis.

Materials & Methods: Co-word analysis, strategic diagram and social network analysis were employed.

Results: In line with strategic diagrams, ADSC differentiation and transplantation as main undeveloped themes in 2003-2007 were partially replaced by regeneration medicine and ADSCs for myocardial infarction in 2008 to 2012, and then partially replaced by miRNAs in ADSC genetics and nerve regeneration in 2013 to 2017. Read More

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December 2018

It's Getting Hot in Here: Targeting Cancer Stem-like Cells with Hyperthermia.

J Stem Cell Transplant Biol 2017 29;2(2). Epub 2017 Dec 29.

Department of Stem Cell Biology and Regenerative Medicine, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, NE30, Cleveland, OH 44195, USA.

Cancer stem-like cells (CSCs) are a subset of cancer cells that are resistant to conventional radiotherapy and chemotherapy. As such, CSCs have been recognized as playing a large role in tumor initiation and recurrence. Although hyperthermia is broadly used in cancer treatment either alone or in combination with radio- or chemo-therapy, its potential to target CSCs is not well understood. Read More

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December 2017

New neurons use Slit-Robo signaling to migrate through the glial meshwork and approach a lesion for functional regeneration.

Sci Adv 2018 Dec 12;4(12):eaav0618. Epub 2018 Dec 12.

Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan.

After brain injury, neural stem cell-derived neuronal precursors (neuroblasts) in the ventricular-subventricular zone migrate toward the lesion. However, the ability of the mammalian brain to regenerate neuronal circuits for functional recovery is quite limited. Here, using a mouse model for ischemic stroke, we show that neuroblast migration is restricted by reactive astrocytes in and around the lesion. Read More

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December 2018

DNAX-activating protein 10 co-stimulation enhances the anti-tumor efficacy of chimeric antigen receptor T cells.

Oncoimmunology 2019 2;8(1):e1509173. Epub 2018 Nov 2.

Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Chimeric antigen receptor (CAR) T cell immunotherapies have shown remarkable efficacy in treating multiple types of hematological malignancies but are not sufficiently effective at treating solid tumors. NKG2D is a strong activating receptor for NK cells and a co-stimulatory receptor for T cells. NKG2D signal transduction depends on DNAX-activating protein 10 (DAP10). Read More

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November 2018
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Combination PD-1 blockade and irradiation of brain metastasis induces an effective abscopal effect in melanoma.

Oncoimmunology 2019 11;8(1):e1507669. Epub 2018 Oct 11.

Department of Immunology, Lerner Research Institute, Cleveland, OH, USA.

Nearly half of melanoma patients develop brain metastases during the course of their disease. Despite advances in both localized radiation and systemic immunotherapy, brain metastases remain difficult to treat, with most patients surviving less than 5 months from the time of diagnosis. While both treatment regimens have individually shown considerable promise in treating metastatic melanoma, there is interest in combining these strategies to take advantage of potential synergy. Read More

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October 2018
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A combined microfluidic-transcriptomic approach to characterize the extravasation potential of cancer cells.

Oncotarget 2018 Nov 16;9(90):36110-36125. Epub 2018 Nov 16.

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, Singapore.

The reciprocal interaction between circulating tumor cells (CTCs) and tissue-specific cells is influential for the progression of metastases. In particular, the process of extravasation relies on the complex cross-talk between cancer cells and other cellular players such as the endothelium and the secondary tissue. However, most studies only focus on one heterotypic cell-cell interaction and often lack of physiological relevance. Read More

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November 2018

Purinergic signaling regulates mobilization of hematopoietic stem cells.

Oncotarget 2018 Nov 16;9(90):36052-36054. Epub 2018 Nov 16.

Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA; Department of Regenerative Medicine Warsaw Medical University, Warsaw, Poland.

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November 2018

Racial disparities in the survival time of patients with hepatocellular carcinoma and intrahepatic cholangiocarcinoma between Chinese patients and patients of other racial groups: A population-based study from 2004 to 2013.

Oncol Lett 2018 Dec 5;16(6):7102-7116. Epub 2018 Oct 5.

Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.

The aim of the present study was to investigate the racial disparities in the presentation, treatment and survival time of patients with hepatocellular carcinoma (HCC) or intrahepatic cholangiocarcinoma (ICC) between Chinese and other racial groups from the Surveillance, Epidemiology, and End Results (SEER) database between January 1st 2004, and December 31st 2013. Key covariates, including clinical presentation, treatment and survival time, were recorded and compared, demonstrating the racial differences. Kaplan-Meier analysis and Cox regression models were performed to identify these disparities in survival time. Read More

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December 2018

FGF10 Signaling in Heart Development, Homeostasis, Disease and Repair.

Front Genet 2018 28;9:599. Epub 2018 Nov 28.

Aix-Marseille Univ, INSERM, MMG, U1251, Marseille, France.

Essential muscular organ that provides the whole body with oxygen and nutrients, the heart is the first organ to function during embryonic development. Cardiovascular diseases, including acquired and congenital heart defects, are the leading cause of mortality in industrialized countries. Fibroblast Growth Factors (FGFs) are involved in a variety of cellular responses including proliferation, differentiation, and migration. Read More

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November 2018

Use of a Primary Epithelial Cell Screening Tool to Investigate Phage Therapy in Cystic Fibrosis.

Front Pharmacol 2018 28;9:1330. Epub 2018 Nov 28.

Telethon Kids Institute, Perth, WA, Australia.

Antimicrobial-resistant microbes are an increasing threat to human health. In cystic fibrosis (CF), airway infections with remain a key driver of lung damage. With few new antibiotics on the development horizon, alternative therapeutic approaches are needed against antimicrobial-resistant pathogens. Read More

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November 2018
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Combined endodontic therapy and peri-radicular regenerative surgery in the treatment of dens invaginatus type III associated with apicomarginal defect.

J Conserv Dent 2018 Nov-Dec;21(6):696-700

Private Practice, Noida, India.

Dens invaginatus (DI) is a dental developmental abnormality, probably resulting from an infolding of the dental papilla during tooth development. The following case report presents a case of Type III DI with apicomarginal defect and peri-radicular lesion, diagnosed with cone-beam computed tomography scan and treated with combined orthograde endodontic therapy and peri-radicular regenerative surgery. Follow-up examination at 2 years revealed uneventful healing and improvement in the status of the tooth-supporting structures. Read More

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December 2018

Report of a case of platelet-rich fibrin-mediated revascularization of immature 12 with histopathological evaluation.

J Conserv Dent 2018 Nov-Dec;21(6):691-695

Department of Conservative Dentistry and Endodontics, Dr. R. Ahmed Dental College and Hospital, Kolkata, West Bengal, India.

The procedure of regeneration has shown promising results in the treatment of nonvital teeth with immature apices. However, the tissue being regenerated can only be confirmed through histological examination. This report describes an immature nonvital 12 with apical pathology which was treated via revascularization using 3% NaOCl and 17% ethylenediaminetetraacetic acid as irrigants; Ca(OH)2 as intracanal medicament and platelet-rich fibrin (PRF) as scaffold. Read More

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December 2018

Reviving regenerative urology.

Nat Rev Urol 2018 Dec 13. Epub 2018 Dec 13.

Klinikum Lippe, Klinik für Urologie, Detmold, Germany.

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December 2018

Hippo-YAP/TAZ signalling in organ regeneration and regenerative medicine.

Nat Rev Mol Cell Biol 2018 Dec 13. Epub 2018 Dec 13.

VIB Center for Cancer Biology, and KU Leuven Department of Oncology, University of Leuven, Leuven, Belgium.

The Hippo pathway and its downstream effectors, the transcriptional co-activators Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ), regulate organ growth and cell plasticity during animal development and regeneration. Remarkably, experimental activation of YAP/TAZ in the mouse can promote regeneration in organs with poor or compromised regenerative capacity, such as the adult heart and the liver and intestine of old or diseased mice. However, therapeutic YAP/TAZ activation may cause serious side effects. Read More

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December 2018

Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.

Nat Commun 2018 Dec 13;9(1):5300. Epub 2018 Dec 13.

Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan.

The liver possesses a high regenerative capacity. Liver regeneration is a compensatory response overcoming disturbances of whole-body homeostasis provoked by organ defects. Here we show that a vagus-macrophage-hepatocyte link regulates acute liver regeneration after liver injury and that this system is critical for promoting survival. Read More

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December 2018
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Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.

Nat Commun 2018 Dec 13;9(1):5304. Epub 2018 Dec 13.

Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY, 10029, USA.

Wnt/Wg-signalling is critical signalling in all metazoans. Recent studies suggest that IFT-A proteins and Kinesin-2 modulate canonical Wnt/Wg-signalling independently of their ciliary role. Whether they function together in Wnt-signalling and their mechanistic role in the pathway remained unresolved. Read More

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December 2018

Clinical trial design and new therapies for pulmonary arterial hypertension.

Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.

San Diego School of Medicine, University of California, La Jolla, CA, USA.

Until 20 years ago the treatment of pulmonary arterial hypertension (PAH) was based on case reports and small series, and was largely ineffectual. As a deeper understanding of the pathogenesis and pathophysiology of PAH evolved over the subsequent two decades, coupled with epidemiological studies defining the clinical and demographic characteristics of the condition, a renewed interest in treatment development emerged through collaborations between international experts, industry and regulatory agencies. These efforts led to the performance of robust, high-quality clinical trials of novel therapies that targeted putative pathogenic pathways, leading to the approval of more than 10 novel therapies that have beneficially impacted both the quality and duration of life. Read More

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December 2018
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B cell lymphomas present immunoglobulin neoantigens.

Blood 2018 Dec 13. Epub 2018 Dec 13.

Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA, United States

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December 2018

Let it RE:IN: integrating experimental observations to predict pluripotency network behaviour.

EMBO J 2018 Dec 13. Epub 2018 Dec 13.

Department of Anatomy and Developmental Biology, Monash University, Clayton, Vic., Australia.

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December 2018

Recycle the dental fairy's package: overview of dental pulp stem cells.

Stem Cell Res Ther 2018 Dec 13;9(1):347. Epub 2018 Dec 13.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, Hubei, China.

Adult stem cells are excellent cell resource for cell therapy and regenerative medicine. Dental pulp stem cells (DPSCs) have been discovered and well known in various application. Here, we reviewed the history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage. Read More

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December 2018

The combination of bFGF and CHIR99021 maintains stable self-renewal of mouse adult retinal progenitor cells.

Stem Cell Res Ther 2018 Dec 13;9(1):346. Epub 2018 Dec 13.

Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, 200072, China.

Background: Millions of people are affected with retinal diseases that eventually cause blindness, and retinal progenitor cell (RPC) transplantation is a promising therapeutic avenue. However, RPC expansion and the underlying regulation mechanisms remain elusive.

Methods: Adult mouse neural RPCs (mNRPCs) were isolated and amplified with the combination of basic fibroblast growth factor (bFGF) and glycogen synthase kinase 3 (GSK3) inhibitor CHIR99021. Read More

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December 2018

A specific protocol of autologous bone marrow concentrate and platelet products versus exercise therapy for symptomatic knee osteoarthritis: a randomized controlled trial with 2 year follow-up.

J Transl Med 2018 Dec 13;16(1):355. Epub 2018 Dec 13.

CAPHRI, School of Public Health and Primary Care, Maastricht University, Maastricht, Netherlands.

Background: Cell-based therapies have shown promise for the treatment of knee osteoarthritis (OA). The current study compared exercise therapy to autologous bone marrow concentrate (BMC) and platelet products for knee OA treatment.

Methods: Patients with symptomatic knee OA (N = 48) were randomized into either an exercise therapy control group or treatment group with injection of autologous BMC and platelet products. Read More

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December 2018

Epigenetic Regulation of Skin Cells in Natural Aging and Premature Aging Diseases.

Cells 2018 Dec 12;7(12). Epub 2018 Dec 12.

Molecular and Cell Biology Laboratory, Istituto Dermopatico dell'Immacolata, IDI-IRCCS, Via dei Monti di Creta 104, 00167 Rome, Italy.

Skin undergoes continuous renewal throughout an individual's lifetime relying on stem cell functionality. However, a decline of the skin regenerative potential occurs with age. The accumulation of senescent cells over time probably reduces tissue regeneration and contributes to skin aging. Read More

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December 2018

Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany.

Thyroid cancer is the most abundant tumor of the endocrine organs. Poorly differentiated thyroid cancer is still difficult to treat. Human cells exposed to long-term real (r-) and simulated (s-) microgravity (µ) revealed morphological alterations and changes in the expression profile of genes involved in several biological processes. Read More

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December 2018

Regenerative Capacity of Endogenous Factor: Growth Differentiation Factor 11; a New Approach of the Management of Age-Related Cardiovascular Events.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Equipe d'Accueil (EA 7460): Physiopathologie et Epidémiologie Cérébro-Cardiovasculaires (PEC2), Université de Bourgogne-Franche Comté, Faculté des Sciences de Santé, 7 Bd Jeanne d'Arc, 21000 Dijon, France.

Aging is a complicated pathophysiological process accompanied by a wide array of biological adaptations. The physiological deterioration correlates with the reduced regenerative capacity of tissues. The rejuvenation of tissue regeneration in aging organisms has also been observed after heterochronic parabiosis. Read More

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December 2018