1,191 results match your criteria Regenerative Medicine [Journal]


Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.

Regen Med 2019 Mar 27;14(3):165-177. Epub 2019 Feb 27.

Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

To determine the efficiency of mesenchymal stem cells (MSCs) of different sources on airway epithelial cells regeneration and track where and to what extent transplanted MSCs home to injured tissues. We performed DiO-labeled human bone marrow-derived MSCs (hBMSCs) or human chorionic villi-derived MSCs transplantation studies using naphthalene-induced airway injury animal models. Compared with human chorionic villi-derived MSCs, hBMSCs facilitated airway epithelium regeneration faster and better from day 5 after transplantation; moreover, more transplanted hBMSCs distributed in injured lung tissues at the early stage of postinjury, which was mediated by C-X-C motif chemokine ligand 12. Read More

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http://dx.doi.org/10.2217/rme-2018-0152DOI Listing

Industry updates from the field of stem cell research and regenerative medicine in February 2019.

Authors:
Dusko Ilic

Regen Med 2019 Apr 16. Epub 2019 Apr 16.

Stem Cell Laboratories, Guy's Assisted Conception Unit, Department of Women & Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in February 2019. Read More

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https://www.futuremedicine.com/doi/10.2217/rme-2019-0042
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http://dx.doi.org/10.2217/rme-2019-0042DOI Listing
April 2019
2 Reads

The use of mesenchymal stem cells for the treatment of progressive retinal diseases: a review.

Regen Med 2019 Apr 12. Epub 2019 Apr 12.

Polski Bank Komórek Macierzystych SA (FamiCord Group), Jana Pawła II 29, 00-867 Warsaw, Poland.

Some ocular diseases, such as dystrophies, retinal and macular degeneration, optic nerve atrophy, and Stargardt disease, are progressive and irreversible. In this review, we focus on the use of mesenchymal stem cells (MSCs) in the treatment of these diseases. In animal studies, MSC transplantation significantly delayed retinal degeneration, led to the regeneration of cone cells, and supported the survival of retinal ganglion cells and axon regeneration. Read More

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http://dx.doi.org/10.2217/rme-2019-0022DOI Listing

Industry updates from the field of stem cell research and regenerative medicine in January 2019.

Authors:
Dusko Ilic

Regen Med 2019 Apr 2. Epub 2019 Apr 2.

Department of Women & Children's Health, Stem Cell Laboratories, Guy's Assisted Conception Unit, Faculty of Life Sciences & Medicine, King's College London, London, UK.

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in January 2019. Read More

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http://dx.doi.org/10.2217/rme-2019-0031DOI Listing
April 2019
1 Read

Stem cell culture conditions and stability: a joint workshop of the PluriMes Consortium and Pluripotent Stem Cell Platform.

Regen Med 2019 Mar 2;14(3):243-255. Epub 2019 Apr 2.

STEMCELL Technologies, Vancouver, BC, V6A 1B6, Canada.

Human stem cells have the potential to transform medicine. However, hurdles remain to ensure that manufacturing processes produce safe and effective products. A thorough understanding of the biological processes occurring during manufacture is fundamental to assuring these qualities and thus, their acceptability to regulators and clinicians. Read More

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https://www.futuremedicine.com/doi/10.2217/rme-2019-0001
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http://dx.doi.org/10.2217/rme-2019-0001DOI Listing
March 2019
5 Reads

Combination of stem cell and gene therapy ameliorates symptoms in Huntington's disease mice.

NPJ Regen Med 2019 26;4. Epub 2019 Mar 26.

1Department of Human Genetics, Emory University School of Medicine, Atlanta, GA USA.

Huntington's disease (HD) is a dominantly inherited monogenetic disorder characterized by motor and cognitive dysfunction due to neurodegeneration. The disease is caused by the polyglutamine (polyQ) expansion at the 5' terminal of the exon 1 of the huntingtin () gene, , which results in the accumulation of mutant HTT (mHTT) aggregates in neurons and cell death. The monogenetic cause and the loss of specific neural cell population make HD a suitable candidate for stem cell and gene therapy. Read More

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http://dx.doi.org/10.1038/s41536-019-0066-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435637PMC

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in November 2018.

Regen Med 2019 Mar 26;14(3):139-144. Epub 2019 Mar 26.

MSc Stem Cell & Regenerative Therapies: from Bench to Market, Faculty of Life Sciences & Medicine, King's College London, London, UK.

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http://dx.doi.org/10.2217/rme-2019-0007DOI Listing

Encapsulation of macrophages enhances their retention and angiogenic potential.

NPJ Regen Med 2019 20;4. Epub 2019 Mar 20.

1King's College London, Academic Department of Vascular Surgery, School of Cardiovascular Medicine & Sciences, BHF Centre for Regenerative Medicine and BHF Centre of Excellence and the Biomedical Research Centre at Guy's & St Thomas' NHS Foundation Trust and King's College London, London, UK.

Cell therapies to treat critical limb ischaemia have demonstrated only modest results in clinical trials, and this has been partly attributed to poor cell retention following their delivery directly into the ischaemic limb. The aim of this study was to determine whether alginate encapsulation of therapeutic pro-angio/arteriogenic macrophages enhances their retention and ultimately improves limb perfusion. A reproducible GMP-compliant method for generating 300 µm alginate capsules was developed to encapsulate pro-angio/arteriogenic macrophages. Read More

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http://dx.doi.org/10.1038/s41536-019-0068-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426993PMC

Ask the experts: current obstacles and future developments in cell culture.

Regen Med 2019 Mar 19;14(3):145-150. Epub 2019 Mar 19.

MRC Center for Regenerative Medicine, University of Edinburgh, Edinburgh, UK.

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http://dx.doi.org/10.2217/rme-2019-0029DOI Listing

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in December 2018.

Authors:
Ilic Dusko

Regen Med 2019 Mar 19. Epub 2019 Mar 19.

Stem Cell Laboratories, Guy's Assisted Conception Unit, Department of Women & Children's Health, Faculty of Life Sciences & Medicine, King's College London, SE1 9RT, London, UK.

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http://dx.doi.org/10.2217/rme-2019-0013DOI Listing

Variable outcomes of human heart attack recapitulated in genetically diverse mice.

NPJ Regen Med 2019 4;4. Epub 2019 Mar 4.

1Australian Regenerative Medicine Institute, Monash University, Clayton, VIC Australia.

Clinical variation in patient responses to myocardial infarction (MI) has been difficult to model in laboratory animals. To assess the genetic basis of variation in outcomes after heart attack, we characterized responses to acute MI in the Collaborative Cross (CC), a multi-parental panel of genetically diverse mouse strains. Striking differences in post-MI functional, morphological, and myocardial scar features were detected across 32 CC founder and recombinant inbred strains. Read More

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http://dx.doi.org/10.1038/s41536-019-0067-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399323PMC
March 2019
4 Reads

Engineering, differentiation and harvesting of human adipose-derived stem cell multilayer cell sheets.

Regen Med 2019 Mar 4;14(3):151-163. Epub 2019 Mar 4.

Department of Medicine, LA BioMed at Harbor UCLA Medical Center, Torrance, CA 90502, USA.

The study goals were to engineer and harvest scaffold-free undifferentiated/differentiated multilayer human adipose-derived stem cell (hADSC) cell sheets, in absence of treatment.  The hADSC are seeded in 35 mm culture dishes. At confluence or when multilayer cell sheets are formed, hADSC are treated with predefined differentiation culture media (adipocyte, chondrocyte and osteoblast). Read More

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http://dx.doi.org/10.2217/rme-2018-0053DOI Listing
March 2019
3 Reads

Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis.

NPJ Regen Med 2019 21;4. Epub 2019 Feb 21.

1Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193 Japan.

Current cartilage regenerative therapies are not fully effective in treating osteoarthritis of the knee (OAK). We have developed chondrocyte sheets for autologous transplantation and tested these in in vitro and in vivo preclinical studies, and have reported that the transplantation of chondrocyte sheets promoted hyaline cartilage repair in rat, rabbit, and minipig models. However, autologous transplantation of chondrocyte sheets has yet to be reported in humans. Read More

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http://dx.doi.org/10.1038/s41536-019-0069-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384900PMC
February 2019
1 Read

Autologous nanofat transplantation accelerates foot wound healing in diabetic rats.

Regen Med 2019 Mar 27;14(3):231-241. Epub 2019 Feb 27.

Department of Plastic & Reconstructive Surgery, Sir Run Run Shaw Hospital, Medical College, Zhejiang University, Hang Zhou 310016, PR China.

This study explored the effects of local transplantation of autologous nanofat in the treatment of rats with diabetic foot wounds. Nanofat was transplanted into the left foot wound of diabetic rats. Phosphate-buffered saline injection in the right served as control. Read More

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http://dx.doi.org/10.2217/rme-2018-0169DOI Listing
March 2019
3 Reads

Exploration of 2-deoxy-D-ribose and 17β-Estradiol as alternatives to exogenous VEGF to promote angiogenesis in tissue-engineered constructs.

Regen Med 2019 Mar 22;14(3):179-197. Epub 2019 Feb 22.

Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK.

In this study, we explored the angiogenic potential and proangiogenic concentration ranges of 2-deoxy-D-ribose (2dDR) and 17β-Estradiol (E2) in comparison with VEGF. The 2dDR and E2 were then loaded into tissue engineering (TE) scaffolds to investigate their proangiogenic potential when released from fibers. chick chorioallantoic membrane (CAM) assay was used to evaluate angiogenic activity of 2dDR and E2. Read More

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http://dx.doi.org/10.2217/rme-2018-0068DOI Listing
March 2019
1 Read
2.786 Impact Factor

Regenerative medicine curriculum for next-generation physicians.

NPJ Regen Med 2019 7;4. Epub 2019 Feb 7.

2Mayo Clinic Center for Regenerative Medicine, Rochester, MN USA.

Regenerative sciences are poised to transform clinical practice. The quest for regenerative solutions has, however, exposed a major gap in current healthcare education. A call for evidence-based adoption has underscored the necessity to establish rigorous regenerative medicine educational programs early in training. Read More

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http://dx.doi.org/10.1038/s41536-019-0065-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367326PMC
February 2019
1 Read

Autologous plasma rich in growth factors technology for isolation and ex vivo expansion of human dental pulp stem cells for clinical translation.

Regen Med 2019 02 15;14(2):97-111. Epub 2019 Feb 15.

BTI - Biotechnology Institute, Regenerative Medicine Department, Vitoria 01007, Spain.

Aim: This study investigated the use of the autologous technology of plasma rich in growth factors (PRGF) as a human-based substitute to fetal bovine serum (FBS) in the culture of human dental pulp stem cells.

Materials & Methods: Stem cell characterization was performed. Analysis of isolation, proliferation, migration, trilineage differentiation, senescence and cryopreservation were compared between FBS and PRGF. Read More

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http://dx.doi.org/10.2217/rme-2018-0066DOI Listing
February 2019
1 Read

Bone tissue engineering in the greater omentum is enhanced by a periosteal transplant in a miniature pig model.

Regen Med 2019 02 15;14(2):127-138. Epub 2019 Feb 15.

Department of Oral & Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, 24105 Kiel, Germany.

Aim: Reconstruction of bone defects with autologous grafts has certain disadvantages. The aim of this study is to introduce a new type of living bioreactor for engineering of bone flaps and to evaluate the effect of different barrier membranes.

Materials & Methods: Scaffolds loaded with bone morphogenetic proteins and bone marrow aspirate wrapped with either a collagen membrane or a periosteal flap were implanted in the greater omentum of miniature pigs. Read More

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http://dx.doi.org/10.2217/rme-2018-0031DOI Listing
February 2019
1 Read

Adipose-derived mesenchymal stem cell therapy in the treatment of knee osteoarthritis: a randomized controlled trial.

Regen Med 2019 Mar 14;14(3):213-230. Epub 2019 Feb 14.

Magellan Stem Cells, VIC, Australia.

To evaluate the efficacy of autologous adipose-derived mesenchymal stem cell (ADMSC) therapy on pain, function and disease modification in knee osteoarthritis. 30 participants with symptomatic knee osteoarthritis were randomized into three groups. Two treatment groups received intra-articular ADMSC therapy consisting of either a single injection (100 × 10 ADMSCs) or two injections (100 × 10 ADMSCs at baseline and 6 months). Read More

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http://dx.doi.org/10.2217/rme-2018-0161DOI Listing
March 2019
2 Reads

Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.

Regen Med 2019 Mar 14;14(3):199-211. Epub 2019 Feb 14.

Orthobiologic Laboratory, MedStar Union Memorial Hospital, Baltimore, MD, USA.

This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs). After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (Allo). Allograft coated with copolymers alone (Allo), as controls, or Allo was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3. Read More

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http://dx.doi.org/10.2217/rme-2018-0063DOI Listing
March 2019
1 Read

Evaluating the efficacy of tissue-engineered human amniotic membrane in the treatment of myocardial infarction.

Regen Med 2019 02 11;14(2):113-126. Epub 2019 Feb 11.

Pediatric Urology & Regenerative Medicine Research Center, Section of Tissue Engineering & Stem Cells Therapy, Pediatric Center of Excellence, Tehran University of Medical Sciences, Children's Hospital Medical Center, Tehran 1419433151, Iran.

Aim: The aim of this study was to evaluate the efficacy of tissue-engineered amniotic membrane (AM) in the treatment of myocardial infarction lesions.

Materials & Methods: 20 rats were subjected to coronary arterial ligation in order to induce myocardial infarction injury. Decellularized human AMs were seeded with 2 × 10 adipose-derived mesenchymal stem cells and were implanted in the infarcted hearts. Read More

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http://dx.doi.org/10.2217/rme-2018-0024DOI Listing
February 2019
8 Reads
2.786 Impact Factor

An insight into the whole transcriptome profile of four tissue-specific human mesenchymal stem cells.

Regen Med 2019 Jan 31. Epub 2019 Jan 31.

Cell Therapy Center, The University of Jordan, Amman, Jordan.

Aim: Variations in the clinical outcomes using mesenchymal stem cells (MSCs) treatments exist, reflecting different origins and niches. To date, there is no consensus on the best source of MSCs most suitable to treat a specific disease.

Methods: Total transcriptome analysis of human MSCs was performed. Read More

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http://dx.doi.org/10.2217/rme-2018-0137DOI Listing
January 2019
3 Reads

Ciliary neurotrophic factor stimulates cardioprotection and the proliferative activity in the adult zebrafish heart.

NPJ Regen Med 2019 24;4. Epub 2019 Jan 24.

Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland.

Unlike mammals, adult zebrafish can regenerate their hearts after injury via proliferation of cardiomyocytes. The cell-cycle entry of zebrafish cardiac cells can also be stimulated through preconditioning by thoracotomy, a chest incision without myocardial damage. To identify effector genes of heart preconditioning, we performed transcriptome analysis of ventricles from thoracotomized zebrafish. Read More

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http://dx.doi.org/10.1038/s41536-019-0064-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345746PMC
January 2019
3 Reads

Preparing regenerative therapies for clinical application: proposals for responsible translation.

Regen Med 2019 02 17;14(2):77-84. Epub 2019 Jan 17.

Mayo Clinic Center for Regenerative Medicine, 200 First Street, SW, Rochester, MN 55905, USA.

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https://www.futuremedicine.com/doi/10.2217/rme-2018-0163
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http://dx.doi.org/10.2217/rme-2018-0163DOI Listing
February 2019
23 Reads

Preparing for first human trial of induced pluripotent stem cell-derived cells for Parkinson's disease: an interview with Jun Takahashi.

Authors:
Jun Takahashi

Regen Med 2019 02 15;14(2):93-95. Epub 2019 Jan 15.

Center for iPS Cell Research & Application (CiRA), Kyoto University, Kyoto, Japan.

Professor Jun Takahashi received his MD from Kyoto University in 1986 and his PhD in neurosurgery and neurobiology from Kyoto University Graduate School of Medicine in 1993 (both Kyoto, Japan). He then did a postdoctoral fellowship at the Salk Institute (Dr Fred Gage's Laboratory, CA, USA) where he started research work on neural stem cells. Since returning to Kyoto University Hospital in 1997, he has been investigating functional neurosurgery including deep brain stimulation and cell-based therapies for Parkinson's disease. Read More

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http://dx.doi.org/10.2217/rme-2018-0158DOI Listing
February 2019
5 Reads

Relative contributions of adipose-resident CD146 pericytes and CD34 adventitial progenitor cells in bone tissue engineering.

NPJ Regen Med 2019 7;4. Epub 2019 Jan 7.

1Department of Pathology, Johns Hopkins University, 21205 Baltimore, MD USA.

Pericytes and other perivascular stem/stromal cells are of growing interest in the field of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. Read More

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http://dx.doi.org/10.1038/s41536-018-0063-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323123PMC
January 2019
4 Reads

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in October 2018.

Authors:
Dusko Ilic

Regen Med 2019 02 8;14(2):85-92. Epub 2019 Jan 8.

Stem Cell Laboratories, Guy's Assisted Conception Unit, Department of Women & Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

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https://www.futuremedicine.com/doi/10.2217/rme-2018-0147
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http://dx.doi.org/10.2217/rme-2018-0147DOI Listing
February 2019
7 Reads

Skeletal muscle stem cells in comfort and stress.

NPJ Regen Med 2018 21;3:24. Epub 2018 Dec 21.

1Stem Cells and Development, Department of Developmental & Stem Cell Biology, Institut Pasteur, 75015 Paris, France.

Investigations on developmental and regenerative myogenesis have led to major advances in decrypting stem cell properties and potential, as well as their interactions within the evolving niche. As a consequence, regenerative myogenesis has provided a forum to investigate intrinsic regulators of stem cell properties as well as extrinsic factors, including stromal cells, during normal growth and following injury and disease. Here we review some of the latest advances in the field that have exposed fundamental processes including regulation of stress following trauma and ageing, senescence, DNA damage control and modes of symmetric and asymmetric cell divisions. Read More

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http://dx.doi.org/10.1038/s41536-018-0062-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303387PMC
December 2018
2 Reads

Sustained release of decorin to the surface of the eye enables scarless corneal regeneration.

NPJ Regen Med 2018 21;3:23. Epub 2018 Dec 21.

2School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT UK.

Disorganization of the transparent collagenous matrix in the cornea, as a consequence of a variety of infections and inflammatory conditions, leads to corneal opacity and sight-loss. Such corneal opacities are a leading cause of blindness, according to the WHO. Public health programs target prevention of corneal scarring, but the only curative treatment of established scarring is through transplantation. Read More

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http://www.nature.com/articles/s41536-018-0061-4
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http://dx.doi.org/10.1038/s41536-018-0061-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303295PMC
December 2018
12 Reads

Limbal niche cells: a novel feeder cell for autologous cultivated oral mucosal epithelial transplantation.

Regen Med 2019 01 21;14(1):49-62. Epub 2018 Dec 21.

Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Aim: To establish a culture system using conspecific limbal niche cells (LNCs) as feeders for autologous cultivated oral mucosal epithelial transplantation (COMET).

Materials & Methods: Rabbit oral epithelial sheets, harvested from culture systems containing LNCs or 3T3 cells, were transplanted onto limbal stem cell-deficient rabbit eyes (COMET-3T3 or COMET-LNCs).

Results: After COMET, corneas were relatively restored, with the exception of mild neovascularization in one cornea of the COMET-3T3 group. Read More

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http://dx.doi.org/10.2217/rme-2018-0122DOI Listing
January 2019
3 Reads

Introducing Volume 14 of Regenerative Medicine.

Authors:
Adam Price-Evans

Regen Med 2019 01 20;14(1):1-5. Epub 2018 Dec 20.

Future Science Group, Unitec House, 2 Albert Place, London N3 1QB, UK.

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http://dx.doi.org/10.2217/rme-2018-0167DOI Listing
January 2019
2 Reads

The effect of mesenchymal stem cell-secreted factors on airway epithelial repair.

Regen Med 2019 01 19;14(1):15-31. Epub 2018 Dec 19.

Regenerative Medicine Cluster, Advanced Medical & Dental Institute (IPPT), Universiti Sains Malaysia, 13200 Bertam, Penang, Malaysia.

Aim: This study was aimed to investigate the effect of mesenchymal stem cell (MSC)-secreted factors on airway repair.

Materials & Methods: An indirect in vitro coculture model of injured airway epithelium explant with MSCs was developed. LC-MS/MS analysis was performed to determine factors secreted by MSCs and their involvement in epithelium repair was evaluated by histopathological assessment. Read More

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http://dx.doi.org/10.2217/rme-2018-0020DOI Listing
January 2019
3 Reads

Intravascular cell therapy in stroke: predicting the future trends.

Regen Med 2019 01 18;14(1):63-68. Epub 2018 Dec 18.

Department of Neurology, Institute of Clinical Medicine, University of Eastern Finland, Kuopio, Finland.

This short review examines the trends that have taken place during the last two decades in selecting delivery route and cell product in confirmatory preclinical stroke research. If there had been a major change, this might indicate a strategy with a high potential to enter early-phase clinical studies. The retrospective data show that intravenous cell delivery of mesenchymal stem cells remains the most popular approach in experimental research, clearly dominating early phase clinical studies. Read More

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http://dx.doi.org/10.2217/rme-2018-0037DOI Listing
January 2019
2 Reads

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions September 1-30, 2018.

Authors:
Dusko Ilic

Regen Med 2019 01 18;14(1):7-13. Epub 2018 Dec 18.

Stem Cell Laboratories, Department of Women & Children's Health, Guy's Assisted Conception Unit, Faculty of Life Sciences & Medicine, King's College London, London SE1 9RT, UK.

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http://dx.doi.org/10.2217/rme-2018-0142DOI Listing
January 2019
2 Reads

Efficiency of mesenchymal stem cells in treatment of urinary incontinence: a systematic review on animal models.

Regen Med 2019 01 18;14(1):69-76. Epub 2018 Dec 18.

Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 19839-63113, Iran.

Aim: In recent years, the administration of stem cells has been considered a new option for treatment of urinary incontinence (UI). In the present study, the efficiency of mesenchymal stem cell (MSC) transplantation in the treatment of UI was evaluated.

Methods: Combinations of the key words 'mesenchymal stem cells', 'MSCs', 'urinary incontinence', 'urethral sphincter' and 'involuntary urination' were searched in PubMed and Science Direct databases. Read More

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http://dx.doi.org/10.2217/rme-2018-0040DOI Listing
January 2019
3 Reads

Mapping theme trends and knowledge structure on adipose-derived stem cells: a bibliometric analysis from 2003 to 2017.

Regen Med 2019 01 14;14(1):33-48. Epub 2018 Dec 14.

Department of Physiology, College of Life Science, China Medical University, Shenyang 110122, PR China.

Aim: To investigate the theme trends and knowledge structure of adipose-derived stem cells (ADSCs) related literatures by using bibliometric analysis.

Materials & Methods: Co-word analysis, strategic diagram and social network analysis were employed.

Results: In line with strategic diagrams, ADSC differentiation and transplantation as main undeveloped themes in 2003-2007 were partially replaced by regeneration medicine and ADSCs for myocardial infarction in 2008 to 2012, and then partially replaced by miRNAs in ADSC genetics and nerve regeneration in 2013 to 2017. Read More

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http://dx.doi.org/10.2217/rme-2018-0117DOI Listing
January 2019
3 Reads

The impact of immune response on endochondral bone regeneration.

NPJ Regen Med 2018 29;3:22. Epub 2018 Nov 29.

1Department of Oral and Maxillofacial Surgery & Special Dental Care, University Medical Center Utrecht, Utrecht University, Utrecht, G05.222, PO Box 85500, 3508 GA The Netherlands.

Tissue engineered cartilage substitutes, which induce the process of endochondral ossification, represent a regenerative strategy for bone defect healing. Such constructs typically consist of multipotent mesenchymal stromal cells (MSCs) forming a cartilage template in vitro, which can be implanted to stimulate bone formation in vivo. The use of MSCs of allogeneic origin could potentially improve the clinical utility of the tissue engineered cartilage constructs in three ways. Read More

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http://www.nature.com/articles/s41536-018-0060-5
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http://dx.doi.org/10.1038/s41536-018-0060-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265275PMC
November 2018
16 Reads

Virus-free and oncogene-free induced pluripotent stem cell reprogramming in cord blood and peripheral blood in patients with lung disease.

Regen Med 2018 12 29;13(8):889-915. Epub 2018 Nov 29.

Cellular Engineering Technologies (CET), Inc., Coralville, IA, 52241, USA.

Aim: A virus- and oncogene-free induced pluripotent stem cell (iPSC) reprogramming method was developed with cord blood-derived mononuclear cells (CBDMNC) and peripheral blood mononuclear cells (PBMNC) from patients with genetic lung diseases.

Method: iPSC reprogramming used small molecules, hematopoietic stem cell (HSC) expansion media and episomal vectors that lacked Myc and Lin28.

Results: All iPSC colonies were fully reprogrammed based on SSEA4 expression. Read More

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http://dx.doi.org/10.2217/rme-2018-0041DOI Listing
December 2018
7 Reads

Science-based assessment of source materials for cell-based medicines: report of a stakeholders workshop.

Regen Med 2018 12 29;13(8):935-944. Epub 2018 Nov 29.

Centre for Biological Engineering, Loughborough University, Loughborough, LE11 3TU, UK.

Human pluripotent stem cells (hPSCs) have the potential to transform medicine. However, hurdles remain to ensure safety for such cellular products. Science-based understanding of the requirements for source materials is required as are appropriate materials. Read More

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http://dx.doi.org/10.2217/rme-2018-0120DOI Listing
December 2018
14 Reads
2.786 Impact Factor

Modeling biological and economic uncertainty on cell therapy manufacturing: the choice of culture media supplementation.

Regen Med 2018 12 29;13(8):917-933. Epub 2018 Nov 29.

Department of Bioengineering and iBB - Institute for Bioengineering & Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.

Aim: To evaluate the cost-effectiveness of autologous cell therapy manufacturing in xeno-free conditions.

Materials & Methods: Published data on the isolation and expansion of mesenchymal stem/stromal cells introduced donor, multipassage and culture media variability on cell yields and process times on adherent culture flasks to drive cost simulation of a scale-out campaign of 1000 doses of 75 million cells each in a 400 square meter Good Manufacturing Practices facility.

Results & Conclusion: Passage numbers in the expansion step are strongly associated with isolation cell yield and drive cost increases per donor of $1970 and 2802 for fetal bovine serum and human platelet lysate. Read More

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http://dx.doi.org/10.2217/rme-2018-0034DOI Listing
December 2018
2 Reads

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions 1 July-31 August 2018.

Authors:
Dusko Ilic

Regen Med 2018 12 23;13(8):875-880. Epub 2018 Nov 23.

Stem Cell Laboratories, Guy's Assisted Conception Unit, Department of Women & Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

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https://www.futuremedicine.com/doi/10.2217/rme-2018-0118
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http://dx.doi.org/10.2217/rme-2018-0118DOI Listing
December 2018
12 Reads

Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from non-academic institutions June 1-June 30, 2018.

Authors:
Dusko Ilic

Regen Med 2018 12 19;13(8):867-874. Epub 2018 Nov 19.

Stem Cell Laboratories, Guy's Assisted Conception Unit, Department of Women & Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

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http://dx.doi.org/10.2217/rme-2018-0097DOI Listing
December 2018
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Parallels between vertebrate cardiac and cutaneous wound healing and regeneration.

NPJ Regen Med 2018 7;3:21. Epub 2018 Nov 7.

School of Physiology, Pharmacology and Neuroscience, Faculty of Biomedical Sciences, University of Bristol, Bristol, UK.

The cellular events that contribute to tissue healing of non-sterile wounds to the skin and ischaemic injury to internal organs such as the heart share remarkable similarities despite the differences between these injury types and organs. In adult vertebrates, both injuries are characterised by a complex series of overlapping events involving multiple different cell types and cellular interactions. In adult mammals both tissue-healing processes ultimately lead to the permanent formation of a fibrotic, collagenous scar, which can have varying effects on tissue function depending on the site and magnitude of damage. Read More

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http://www.nature.com/articles/s41536-018-0059-y
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http://dx.doi.org/10.1038/s41536-018-0059-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220283PMC
November 2018
4 Reads

The regenerative rehabilitation collection: a forum for an emerging field.

NPJ Regen Med 2018 19;3:20. Epub 2018 Oct 19.

4Rehabilitation R&D REAP, VA Palo Alto Health Care System, Palo Alto, CA 94304 USA.

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http://dx.doi.org/10.1038/s41536-018-0058-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195606PMC
October 2018
12 Reads

Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair.

Regen Med 2018 12 22;13(8):881-898. Epub 2018 Oct 22.

Department of Research & Development, Burst Biologics, Boise, ID 83705, USA.

Aim: Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp).

Materials & Methods: Luminex multiplex assay and standard cell biology methods were employed. Read More

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http://dx.doi.org/10.2217/rme-2018-0102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439518PMC
December 2018
2 Reads

Trends in clinical trials for articular cartilage repair by cell therapy.

NPJ Regen Med 2018 11;3:17. Epub 2018 Oct 11.

1Department of Regenerative Medicine Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, 1-98, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192 Japan.

Focal and degenerative lesions of articular cartilage greatly reduce the patient's quality of life. Various therapies including surgical treatment have been developed, but a definitive therapy is not yet known. Several cell therapy products have already been developed and are available in the market. Read More

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http://www.nature.com/articles/s41536-018-0055-2
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http://dx.doi.org/10.1038/s41536-018-0055-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181982PMC
October 2018
21 Reads

The convergence of regenerative medicine and rehabilitation: federal perspectives.

NPJ Regen Med 2018 10;3:19. Epub 2018 Oct 10.

Veteran's Administration, Greater Los Angeles Healthcare System, Los Angeles, CA USA.

Regenerative rehabilitation is the synergistic integration of principles and approaches from the regenerative medicine and rehabilitation fields, with the goal of optimizing form and function as well as patient independence. Regenerative medicine approaches for repairing or replacing damaged tissue or whole organs vary from utilizing cells (e.g. Read More

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http://www.nature.com/articles/s41536-018-0056-1
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http://dx.doi.org/10.1038/s41536-018-0056-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180133PMC
October 2018
18 Reads

Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs.

NPJ Regen Med 2018 10;3:18. Epub 2018 Oct 10.

1Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305 USA.

Despite the regenerative capacity of muscle, tissue volume is not restored after volumetric muscle loss (VML), perhaps due to a loss-of-structural extracellular matrix. We recently demonstrated the structural and functional restoration of muscle tissue in a mouse model of VML using an engineered "bioconstruct," comprising an extracellular matrix scaffold (decellularized muscle), muscle stem cells (MuSCs), and muscle-resident cells (MRCs). To test the ability of the cell-based bioconstruct to restore whole-muscle biomechanics, we measured biomechanical parameters in uninjured muscles, muscles injured to produce VML lesions, and in muscles that were injured and then treated by implanting either the scaffolds alone or with bioconstructs containing the scaffolds, MuSCs, and MRCs. Read More

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http://www.nature.com/articles/s41536-018-0057-0
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http://dx.doi.org/10.1038/s41536-018-0057-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180087PMC
October 2018
21 Reads

Human amniotic membrane as differentiating matrix for in vitro chondrogenesis.

Regen Med 2018 10 9;13(7):821-832. Epub 2018 Oct 9.

Centre of Excellence in Molecular Biology, 87 West Canal Bank Road, Thokar Niazbaig Lahore, Punjab, 53700 Pakistan.

Aim: The aim of the present study is to use human amniotic membrane (HAM) for in vitro chondrogenesis of placenta-derived mesenchymal stem cells (MSCs) and umbilical cord-derived MSCs.

Materials & Methods: MSCs from the placenta and umbilical cord were isolated, characterized by immunophenotyping and after analyzing their rate of proliferation, cytotoxicity and viability, chondrogenesis was performed on plastic adherent surface and on HAM.

Results: Successfully isolated and characterized placenta-derived MSCs and umbilical cord-derived MSCs revealed positive expression of MSCs markers CD90, CD73, CD105 and CD49d, while they were negative for CD45. Read More

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https://www.futuremedicine.com/doi/10.2217/rme-2018-0017
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http://dx.doi.org/10.2217/rme-2018-0017DOI Listing
October 2018
7 Reads

Corporate profile: RoosterBio, Inc.

Regen Med 2018 10 5;13(7):753-757. Epub 2018 Oct 5.

RoosterBio, Inc. 5295 Westview Drive, Suite 275, Frederick, MD 21703, USA.

RoosterBio, Inc. (MD, USA) is a privately held stem cell tools and technology company focused on accelerating the development of a sustainable regenerative medicine industry, one customer at a time. RoosterBio's products are high-volume and well-characterized adult human mesenchymal stem/stromal cells (hMSCs) paired with highly engineered media systems. Read More

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http://dx.doi.org/10.2217/rme-2018-0092DOI Listing
October 2018
3 Reads