1,038,682 results match your criteria RNA New York N.Y.[Journal]


Targeted Knock-In Mice with a Human Mutation in GRTH/DDX25 Reveals the Essential Role of Phosphorylated GRTH in Spermatid Development during Spermatogenesis.

Hum Mol Genet 2019 Apr 22. Epub 2019 Apr 22.

Section on Molecular Endocrinology, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) is a testis specific member of the DEAD-box family of RNA helicases expressed in meiotic and haploid germ cells which plays an essential role in spermatogenesis. There are two species of GRTH the 56 kDa non-phospho and 61 kDa phospho forms. Our early studies revealed a missense mutation (R242H) of GRTH in azoospermic men that when expressed in COS1-cells lack the phospho-form of GRTH. Read More

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https://academic.oup.com/hmg/advance-article/doi/10.1093/hmg
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http://dx.doi.org/10.1093/hmg/ddz079DOI Listing
April 2019
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Role of nuclear RNA in regulating chromatin structure and transcription.

Curr Opin Cell Biol 2019 Apr 19;58:120-125. Epub 2019 Apr 19.

MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK. Electronic address:

The importance of three-dimensional chromatin organisation in genome regulation has never been clearer. But in spite of the enormous technological advances to probe chromatin organisation in vivo, there is still a lack of mechanistic understanding of how such an arrangement is achieved. Here we review emerging evidence pointing to an intriguing role of nuclear RNA in shaping large-scale chromatin structure and regulating genome function. Read More

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http://dx.doi.org/10.1016/j.ceb.2019.03.007DOI Listing

Using SHAPE-MaP to probe small molecule-RNA interactions.

Methods 2019 Apr 19. Epub 2019 Apr 19.

Department of Biological Sciences, Auburn University, 120 W. Samford Ave, Rouse Life Sciences Building, Auburn, AL 36849, USA. Electronic address:

RNA is a regulator and catalyst of many cellular processes. Efforts to therapeutically harness RNA began with the discovery of myriad coding and non-coding RNAs and their versatile modes of action. However, due to its dynamic structure and the polar and repetitive nature of its surface, RNA presents a challenging target for drug design. Read More

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http://dx.doi.org/10.1016/j.ymeth.2019.04.009DOI Listing

A novel variant m.641A>T in the mitochondrial MT-TF gene is associated with epileptic encephalopathy in adolescent.

Mitochondrion 2019 Apr 19. Epub 2019 Apr 19.

Department of Inborn Errors of Metabolism, FSBI 'Research Centre for Medical Genetics', Moscow, Russia.

We present a 14-year-old girl with loss of motor functions, tetraplegia, epilepsy and nystagmus, caused by a novel heteroplasmic m.641A>T transition in an evolutionary conserved region of mitochondrial genome, affecting the aminoacyl stem of mitochondrial tRNA-Phe. In silico prediction, respirometry, Western blot and enzymatic analyses in skin fibroblasts support the pathogenicity of the m. Read More

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http://dx.doi.org/10.1016/j.mito.2019.04.004DOI Listing

The characteristic of Arachis duranensis-specific genes and their potential function.

Gene 2019 Apr 19. Epub 2019 Apr 19.

Grassland Agri-husbandry Research Center, Qingdao Agricultural University, Qingdao, China. Electronic address:

Arachis species produce flowers aerially, and then grow into the ground, where they develop into fruits; a feature that is unique to Arachis species. We hypothesized that Arachis species evolved genes specifically involved in the control of aerial flowers and the formation of underground fruits. Arachis duranensis is more resistant to biotic and abiotic stressors. Read More

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http://dx.doi.org/10.1016/j.gene.2019.04.052DOI Listing

Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis.

Insect Biochem Mol Biol 2019 Apr 19. Epub 2019 Apr 19.

Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, B-9000, Ghent, Belgium. Electronic address:

RNA interference (RNAi) refers to the process of suppression of gene expression in eukaryotes, which has a great potential for the control of pest and diseases. Unfortunately, the efficacy of this technology is limited or at best variable in some insects. In the African sweet potato weevil (SPW) Cylas puncticollis, a devastating pest that affects the sweet potato production in Sub-Saharan Africa (SSA), the RNAi response was highly efficient when dsRNA was delivered by injection, but it showed a reduced response by oral feeding. Read More

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http://dx.doi.org/10.1016/j.ibmb.2019.04.001DOI Listing

Genome-wide annotation and comparative analysis of cuticular protein genes in the noctuid pest Spodoptera litura.

Insect Biochem Mol Biol 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing, 400716, China; Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400716, China. Electronic address:

Insect cuticle is considered an adaptable and versatile building material with roles in the construction and function of exoskeleton. Its physical properties are varied, as the biological requirements differ among diverse structures and change during the life cycle of the insect. Although the bulk of cuticle consists basically of cuticular proteins (CPs) associated with chitin, the degree of cuticular sclerotization is an important factor in determining its physical properties. Read More

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http://dx.doi.org/10.1016/j.ibmb.2019.04.012DOI Listing

The altered expression of autophagy-related genes participates in heart failure: NRBP2 and CALCOCO2 are associated with left ventricular dysfunction parameters in human dilated cardiomyopathy.

PLoS One 2019 22;14(4):e0215818. Epub 2019 Apr 22.

Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe), Valencia, Spain.

This study aimed to analyze changes in the expression of autophagy- and phagocytosis-related genes in patients with dilated cardiomyopathy (DCM), especially in relation to left ventricular (LV) dysfunction. Furthermore, transmission electron microscopy of the diseased tissue was carried out to investigate if the gene expression changes are translated into ultrastructural alterations. LV tissue samples from patients with DCM (n = 13) and from controls (CNT; n = 10) were analyzed by RNA-sequencing, whereupon the altered expression (P < 0. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215818PLOS

Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings.

PLoS One 2019 22;14(4):e0215756. Epub 2019 Apr 22.

PATH, Seattle, Washington, United States of America.

Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude their use in low-resource and decentralized healthcare settings. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215756PLOS

Arsenite malignantly transforms human prostate epithelial cells in vitro by gene amplification of mutated KRAS.

PLoS One 2019 22;14(4):e0215504. Epub 2019 Apr 22.

Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America.

Inorganic arsenic is an environmental human carcinogen of several organs including the urinary tract. RWPE-1 cells are immortalized, non-tumorigenic, human prostate epithelia that become malignantly transformed into the CAsE-PE line after continuous in vitro exposure to 5μM arsenite over a period of months. For insight into in vitro arsenite transformation, we performed RNA-seq for differential gene expression and targeted sequencing of KRAS. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215504PLOS

Fatty acid-binding protein 1 increases steer fat deposition by facilitating the synthesis and secretion of triacylglycerol in liver.

PLoS One 2019 22;14(4):e0214144. Epub 2019 Apr 22.

College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.

Castration is an important means of improving the beef quality via increasing fat deposition. However, little is known about the molecular mechanism underlying the fat deposition after castration. Here, the intramuscular fat (IMF) content of the steer group was shown to be much higher than the bull group. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214144PLOS

Destabilization of chromosome structure by histone H3 lysine 27 methylation.

PLoS Genet 2019 Apr 22;15(4):e1008093. Epub 2019 Apr 22.

Environmental Genomics, Christian-Albrechts University, Kiel, Germany.

Chromosome and genome stability are important for normal cell function as instability often correlates with disease and dysfunction of DNA repair mechanisms. Many organisms maintain supernumerary or accessory chromosomes that deviate from standard chromosomes. The pathogenic fungus Zymoseptoria tritici has as many as eight accessory chromosomes, which are highly unstable during meiosis and mitosis, transcriptionally repressed, show enrichment of repetitive elements, and enrichment with heterochromatic histone methylation marks, e. Read More

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http://dx.plos.org/10.1371/journal.pgen.1008093
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http://dx.doi.org/10.1371/journal.pgen.1008093DOI Listing
April 2019
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A small RNA controls bacterial sensitivity to gentamicin during iron starvation.

PLoS Genet 2019 Apr 22;15(4):e1008078. Epub 2019 Apr 22.

Aix Marseille Univ-CNRS, UMR 7243, Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, Marseille, France.

Phenotypic resistance describes a bacterial population that becomes transiently resistant to an antibiotic without requiring a genetic change. We here investigated the role of the small regulatory RNA (sRNA) RyhB, a key contributor to iron homeostasis, in the phenotypic resistance of Escherichia coli to various classes of antibiotics. We found that RyhB induces phenotypic resistance to gentamicin, an aminoglycoside that targets the ribosome, when iron is scarce. Read More

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http://dx.plos.org/10.1371/journal.pgen.1008078
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http://dx.doi.org/10.1371/journal.pgen.1008078DOI Listing
April 2019
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BOFdat: Generating biomass objective functions for genome-scale metabolic models from experimental data.

PLoS Comput Biol 2019 Apr 22;15(4):e1006971. Epub 2019 Apr 22.

Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Genome-scale metabolic models (GEMs) are mathematically structured knowledge bases of metabolism that provide phenotypic predictions from genomic information. GEM-guided predictions of growth phenotypes rely on the accurate definition of a biomass objective function (BOF) that is designed to include key cellular biomass components such as the major macromolecules (DNA, RNA, proteins), lipids, coenzymes, inorganic ions and species-specific components. Despite its importance, no standardized computational platform is currently available to generate species-specific biomass objective functions in a data-driven, unbiased fashion. Read More

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http://dx.doi.org/10.1371/journal.pcbi.1006971DOI Listing

Expression estimation and eQTL mapping for HLA genes with a personalized pipeline.

PLoS Genet 2019 Apr 22;15(4):e1008091. Epub 2019 Apr 22.

Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.

The HLA (Human Leukocyte Antigens) genes are well-documented targets of balancing selection, and variation at these loci is associated with many disease phenotypes. Variation in expression levels also influences disease susceptibility and resistance, but little information exists about the regulation and population-level patterns of expression. This results from the difficulty in mapping short reads originated from these highly polymorphic loci, and in accounting for the existence of several paralogues. Read More

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http://dx.doi.org/10.1371/journal.pgen.1008091DOI Listing

Applications of Next-Generation Sequencing for Large-Scale Pathogen Diagnoses in Soybean.

Plant Dis 2019 Apr 22:PDIS05180905RE. Epub 2019 Apr 22.

Department of Biology, Brandon University, Brandon, Manitoba R78 6A9, Canada.

Soybean () has become an important crop in Manitoba, Canada, with a 10-fold increase in dedicated acreage over the past decade. Given the rapid increase in production, scarce information about foliar diseases present in the province has been recorded. In order to describe the foliar pathogens affecting this legume, we harnessed next-generation sequencing (NGS) to carry out a comprehensive survey across Manitoba in 2016. Read More

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http://dx.doi.org/10.1094/PDIS-05-18-0905-REDOI Listing

CRISPR-Cas: more than ten years and still full of mysteries.

RNA Biol 2019 Apr;16(4):377-379

b Department of Biology II , Ulm University , Ulm , Germany.

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http://dx.doi.org/10.1080/15476286.2019.1591659DOI Listing

Association Between , Its Polymorphism and Ischemia-Modified Albumin in Patients with Rheumatoid Arthritis.

J Interferon Cytokine Res 2019 Apr 22. Epub 2019 Apr 22.

7 Department of Clinical Pathology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.

Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease. We aimed to measure the level of miR-155 and its genetic variant rs767649 in patients with RA and to evaluate their relationship with ischemia-modified albumin (IMA). The study was performed on 79 patients with RA (group I) and 78 healthy control participants (group II). Read More

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http://dx.doi.org/10.1089/jir.2019.0001DOI Listing

A2ML1 and otitis media: novel variants, differential expression and relevant pathways.

Hum Mutat 2019 Apr 22. Epub 2019 Apr 22.

Department of Otolaryngology, University of Colorado School of Medicine (CUSOM), Aurora, Colorado, USA.

A genetic basis for otitis media is established, however the role of rare variants in disease etiology is largely unknown. Previously a duplication variant within A2ML1 was identified as a significant risk factor for otitis media in an indigenous Filipino population and in US children. In this report exome and Sanger sequencing was performed using DNA samples from the indigenous Filipino population, Filipino cochlear implantees, US probands, and Finnish and Pakistani families with otitis media. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/humu.23769
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http://dx.doi.org/10.1002/humu.23769DOI Listing
April 2019
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Exon 2 Skipping Eliminates Gamma-Glutamyl Carboxylase Activity, Indicating a Partial Splicing Defect in a Patient with Vitamin K Clotting Factor Deficiency.

J Thromb Haemost 2019 Apr 22. Epub 2019 Apr 22.

Departments of Cardiovascular, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at CWRU, Cleveland Clinic, 9500 Euclid Avenue NB50, Cleveland, OH, 44195.

Background: Mutations in the gamma-glutamyl carboxylase (GGCX), which is required for vitamin K-dependent (VKD) protein activation, can result in vitamin K clotting factor deficiency (VKCFD1). A recent report described a VKCFD1 patient with a homozygous carboxylase mutation that altered splicing and deleted exon 2 (Δ2GGCX). Only Δ2GGCX RNA was observed in the patient. Read More

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http://dx.doi.org/10.1111/jth.14456DOI Listing

miR-188-5p emerges as an oncomiRNA to promote gastric cancer cell proliferation and migration via upregulation of SALL4.

J Cell Biochem 2019 Apr 22. Epub 2019 Apr 22.

Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.

MicroRNAs (miRNAs) play pivotal roles in modulating key biological processes in gastric cancer (GC). As a newly identified miRNA, the function and potential mechanism of miR-188-5p in GC has not been thoroughly elucidated. Here, quantitative real-time polymerase chain reaction detection showed abnormally higher expression of miR-188-5p in GC cells and tissues. Read More

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http://dx.doi.org/10.1002/jcb.28764DOI Listing

Pantoprazole attenuates tumorigenesis via inhibition of exosomal secretion in a rat model of hepatic precancerous lesion induced by diethylnitrosamine and 2-acetamidofluorene.

J Cell Biochem 2019 Apr 22. Epub 2019 Apr 22.

Department of Medical Biochemistry and Molecular biology, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt.

The present study aimed to evaluate the potential therapeutic effect of pantoprazole, a proton-pump inhibitor, on precancerous lesion (PCL) in rats. diethylnitrosamine and 2-acetylaminofluorene were used to induce PCL in rats, in vivo. The rats were treated with three doses of pantoprazole (100, 50, and 25  mg/kg; three times weekly) during the last 4 weeks of the total 10 weeks of the experiment. Read More

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http://dx.doi.org/10.1002/jcb.28757DOI Listing

Integrative analysis of transcriptome-wide association study data and messenger RNA expression profiles identified candidate genes and pathways for inflammatory bowel disease.

J Cell Biochem 2019 Apr 22. Epub 2019 Apr 22.

Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Collaborative Innovation Center of Endemic Diseases and Health Promotion in Silk Road Region, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.

Inflammatory bowel disease (IBD) is a complex disease, resulting from abnormal immune response to intestinal tract microbiota in genetically susceptible individuals. Crohn's disease and ulcerative colitis (UC) are the two major types of IBD. Transcriptome-wide association study (TWAS) of IBD was first performed using a large-scale genome-wide association study summary data sets of IBD. Read More

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http://dx.doi.org/10.1002/jcb.28744DOI Listing
April 2019
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The MYB transcription factor EMA1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots.

Plant J 2019 Apr 22. Epub 2019 Apr 22.

Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052, Ghent, Belgium.

Plants respond to herbivore or pathogen attacks by activating specific defense programs that include the production of bioactive specialized metabolites to eliminate or deter the attackers. Volatiles play an important role in the interaction of a plant with its environment. Through transcript profiling of jasmonate-elicited Medicago truncatula cells we identified Emission of Methyl Anthranilate (EMA) 1, a MYB transcription factor that is involved in the emission of the volatile compound methyl anthranilate when expressed in M. Read More

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http://dx.doi.org/10.1111/tpj.14347DOI Listing

The poly(A) polymerase PAPS1 interacts with the RNA-directed DNA methylation pathway in sporophyte and pollen development.

Plant J 2019 Apr 22. Epub 2019 Apr 22.

Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476, Potsdam-Golm, Germany.

RNA-based processes play key roles in the regulation of eukaryotic gene expression. This includes both the processing of pre-mRNAs into mature mRNAs ready for translation and RNA-based silencing processes, such as RNA-directed DNA methylation (RdDM). Polyadenylation of pre-mRNAs is one important step in their processing and is carried out by three functionally specialized canonical nuclear poly(A) polymerases in Arabidopsis thaliana. Read More

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http://dx.doi.org/10.1111/tpj.14348DOI Listing

The expression of long non coding RNA genes is associated with expression with polymorphisms of HULC rs7763881 and MALAT1 rs619586 in hepatocellular carcinoma and HBV Egyptian patients.

J Cell Biochem 2019 Apr 22. Epub 2019 Apr 22.

Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Long noncoding RNAs (lncRNAs), highly upregulated liver cancer (HULC), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), lncRNA-AF085935, and lncRNA-uc003wbd have been implicated in hepatocellular carcinoma (HCC). Single-nucleotide polymorphism (SNP) in HULC and MALAT1 are associated with HCC susceptibility. However, association between these SNPs and lncRNA-AF085935 and lncRNA-uc003wbd expression and their potential clinical value in differentiating HCC from both hepatitis B virus (HBV)-infected Egyptian patients and the healthy specimens have not been explored yet. Read More

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http://dx.doi.org/10.1002/jcb.28726DOI Listing
April 2019
3.263 Impact Factor

Detection of differentially expressed genes in discrete single-cell RNA sequencing data using a hurdle model with correlated random effects.

Biometrics 2019 Apr 22. Epub 2019 Apr 22.

Department of Biostatistics, University of Florida, Gainesville, Florida, U.S.A.

Single-cell RNA sequencing (scRNA-seq) technologies are revolutionary tools allowing researchers to examine gene expression at the level of a single cell. Traditionally, transcriptomic data have been analyzed from bulk samples, masking the heterogeneity now seen across individual cells. Even within the same cellular population, genes can be highly expressed in some cells but not expressed (or lowly expressed) in others. Read More

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http://dx.doi.org/10.1111/biom.13074DOI Listing

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses.

J Vis Exp 2019 Apr 4(146). Epub 2019 Apr 4.

UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin; Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie;

The use of recombinant viruses has become crucial in basic or applied virology. Reverse genetics has been proven to be an extremely powerful technology, both to decipher viral replication mechanisms and to study antivirals or provide development platform for vaccines. The construction and manipulation of a reverse genetic system for a negative-strand RNA virus such as a respiratory syncytial virus (RSV), however, remains delicate and requires special know-how. Read More

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http://dx.doi.org/10.3791/59218DOI Listing

Role of programmed cell death ligand-1 expression on prognostic and overall survival of breast cancer: A systematic review and meta-analysis.

Medicine (Baltimore) 2019 Apr;98(16):e15201

Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, China.

Background: Recently, the correlation of immunological checkpoint marker programmed cell death ligand-1 (PD-L1) and the prognosis of various cancers has been a research hotspot. The aim of this study is to examine the prognostic effect of PD-L1 in breast cancer.

Methods: PubMed, EMBASE, Web of Science, the Cochrane Library database were searched for eligible studies and additional hand-searching were reviewed as an augmentation. Read More

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http://dx.doi.org/10.1097/MD.0000000000015201DOI Listing

Upregulation of lncRNA SNHG1 is associated with metastasis and poor prognosis in cancers: A meta-analysis.

Medicine (Baltimore) 2019 Apr;98(16):e15196

Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University.

Background: Accumulating evidence suggested that the expression level of long noncoding RNA small nucleolar RNA host gene 1 (lncRNA SNHG1) was upregulated in various cancers, and high expression of SNHG1 was associated with metastasis and prognosis in patients with cancer.The relationship between SNHG1 expression and metastasis or prognosis in malignant tumors was investigated in this meta-analysis.

Methods: A systematic search was performed in PubMed, Web of Science, and Cochrane Library from inception until May 31, 2018. Read More

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http://dx.doi.org/10.1097/MD.0000000000015196DOI Listing

Evaluation of the Painful Total Knee Arthroplasty.

J Am Acad Orthop Surg 2019 Apr 16. Epub 2019 Apr 16.

From the Beaumont Health, Departments of Orthopaedic Surgery and Research, Royal Oak, MI (Dr. Flierl, Dr. Sobh, and Dr. Baker), the Princeton HealthCare System, Department of Orthopaedic Surgery, Princeton, NJ (Dr. Culp), and the Rush University Medical Center, Midwest Orthopaedics at Rush, Chicago, IL (Dr. Sporer).

Total knee arthroplasty (TKA) has been associated with notable improvements in health-related quality of life of patients with end-stage knee arthritis. Although most patients experience substantial symptomatic relief after TKA, up to 19% of patients are unsatisfied with their outcome. With the dramatic, projected increase in the number of TKAs performed annually, it is crucial to appreciate the various modes of failure associated with this procedure. Read More

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http://dx.doi.org/10.5435/JAAOS-D-18-00083DOI Listing

Central changes in the Kv10.2 potassium channel in stress-induced hypertension rats.

Neuroreport 2019 Apr 18. Epub 2019 Apr 18.

School of Life Sciences, Shanghai University.

Ion channels play as a pivotal role in hypertension in the processes of maintenance of vascular tone and sympathetic excitement of hypertension. The Kv10.2 channel (encoded by the Kcnh5 gene) belongs to the EAG voltage-gated superfamily. Read More

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http://dx.doi.org/10.1097/WNR.0000000000001244DOI Listing

Fatal West Nile Virus Infection in a Virginia Opossum () with Pulmonary Lepidic-Predominant Adenocarcinoma.

J Wildl Dis 2019 Apr 22. Epub 2019 Apr 22.

1 Centre québécois sur la santé des animaux sauvages/Canadian Wildlife Health Cooperative, Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St. Hyacinthe, Quebec J2S 2M2, Canada.

A fatal case of West Nile virus (WNV) infection was diagnosed based on histopathologic findings and the presence of WNV RNA by reverse transcription PCR in the affected organs in a Virginia opossum () in Quebec, Canada in 2017. Disease caused by WNV has not been described in a marsupial species. Read More

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Dynamic cytosine DNA methylation patterns associated with mRNA and siRNA expression profiles in alternate bearing apple trees.

J Agric Food Chem 2019 Apr 22. Epub 2019 Apr 22.

Cytosine DNA methylation plays important roles in plants; it can mediate gene expression to affect plant growth and development. However, little is known about the potential involvement of cytosine DNA methylation in apple, as well as in response to alternate bearing. Here, we performed whole-genome bisulfate sequencing to investigate genomic CG, CHG, and CHH methylation patterns, together with their global mRNA accumulation, and small RNA expression in 'Fuji' apple trees. Read More

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http://dx.doi.org/10.1021/acs.jafc.9b00871DOI Listing

Fluoride interferes with sperm fertilizing ability via downregulated SPAM1, ACR and PRSS21 expression in rat epididymis.

J Agric Food Chem 2019 Apr 22. Epub 2019 Apr 22.

Fluoride is a widespread environmental pollutant which can induce low sperm quality and fertilizing ability, however, the underlying mechanism is still remaining unclear. Hence, we aimed to investigate the influence of fluoride on sperm fertilizing ability via some key proteins in epididymis. For this, forty adult rats were assigned randomly into four groups. Read More

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http://dx.doi.org/10.1021/acs.jafc.9b01114DOI Listing

Effects of Noncanonical Base Pairing on RNA Folding: Structural Context and Spatial Arrangements of G∙A Pairs.

Biochemistry 2019 Apr 22. Epub 2019 Apr 22.

Noncanonical base pairs play important roles in assembling the three-dimensional structures critical to the diverse functions of RNA. These associations contribute to the looped segments that intersperse the canonical double-helical elements within folded, globular RNA molecules. They stitch together various structural elements, serve as recognition elements for other molecules, and act as sites of intrinsic stiffness or deformability. Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00122DOI Listing

Clinical and Molecular Phenotyping in Scleromyxedema Pre- and Post-Treatment with Intravenous Immunoglobulin.

Arthritis Care Res (Hoboken) 2019 Apr 22. Epub 2019 Apr 22.

Johns Hopkins University School of Medicine, Division of Rheumatology, Baltimore, MD.

Background: Scleromyxedema (SMX) is a rare systemic sclerosis mimic that often responds to intravenous immunoglobulin therapy (IVIG), yet the resulting clinical and biochemical changes have not been well characterized.

Methods: Fifteen patients with SMX were recruited for the study. Clinical information and peripheral blood mononuclear cells for flow cytometry were obtained immediately before and again 1-2 weeks after receiving IVIG therapy. Read More

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http://dx.doi.org/10.1002/acr.23908DOI Listing

Dendrimer mediated targeting of siRNA against Polo-like kinase for the treatment of triple negative breast cancer.

J Biomed Mater Res A 2019 Apr 22. Epub 2019 Apr 22.

Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-301, Poland.

Irresponsiveness of triple-negative breast cancer (TNBC) towards conventional therapies has drawn attention towards siRNA therapeutics. In gene delivery, dendrimers are gaining significant attention due to their characteristic features and polo-like kinase (PLK1) is reported as a potential target for TNBC. In this work, phosphorus and polyamidoamine dendrimer (generation 3 and 4 of each type) are explored to address delivery challenges of PLK1 siRNA (siPLK1). Read More

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http://dx.doi.org/10.1002/jbm.a.36701DOI Listing

[miR-494-3p reduces insulin sensitivity in diabetic cardiomyocytes by down-regulation of insulin receptor substrate 1].

Sheng Li Xue Bao 2019 Apr;71(2):271-278

School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.

More and more evidence suggests that microRNA is widely involved in the regulation of cardiovascular function. Our preliminary experiment showed that miR-494-3p was increased in heart of diabetic rats, and miR-494-3p was reported to be related to metabolism such as obesity and exercise. Therefore, this study was aimed to explore the role of miR-494-3p in diabetic myocardial insulin sensitivity and the related mechanism. Read More

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Cross-communication between fibroblasts and cardiomyocytes.

Noncoding RNA Investig 2019 Mar 18;3. Epub 2019 Mar 18.

Cardiovascular Research Center, Department of Cardiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

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http://dx.doi.org/10.21037/ncri.2019.03.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472910PMC

RNA profile of control and nicotine treated Murine Leydig single cell represented in TPM data.

Data Brief 2019 Jun 27;24:103825. Epub 2019 Mar 27.

School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.

Data provided in this article is RNA profile represented in RPKM and RPKM based TPM value for the research article titled Nicotine inhibits Murine Leydig cell differentiation and maturation via regulating Hedgehog signal pathway Jiajie et al., 2019. Nicotine treatment changes the RNA profile of Murine Leydig cells. Read More

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http://dx.doi.org/10.1016/j.dib.2019.103825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454091PMC

Cross-platform Data Analysis Reveals a Generic Gene Expression Signature for Microsatellite Instability in Colorectal Cancer.

Biomed Res Int 2019 17;2019:6763596. Epub 2019 Mar 17.

Faculty of Science, Research Centre for Toxic Compounds in the Environment, Masarykova Univerzita, Kamenice 5, Brno 625 00, Czech Republic.

The dysfunction of the DNA mismatch repair system results in microsatellite instability (MSI). MSI plays a central role in the development of multiple human cancers. In colon cancer, despite being associated with resistance to 5-fluorouracil treatment, MSI is a favourable prognostic marker. Read More

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http://dx.doi.org/10.1155/2019/6763596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441508PMC

Experimentally reduced insulin/IGF-1 signaling in adulthood extends lifespan of parents and improves Darwinian fitness of their offspring.

Evol Lett 2019 Apr 4;3(2):207-216. Epub 2019 Mar 4.

Animal Ecology, Department of Ecology and Genetics Uppsala University Uppsala 752 36 Sweden.

Classical theory maintains that ageing evolves via energy trade-offs between reproduction and survival leading to accumulation of unrepaired cellular damage with age. In contrast, the emerging new theory postulates that ageing evolves because of deleterious late-life hyper-function of reproduction-promoting genes leading to excessive biosynthesis in late-life. The hyper-function theory uniquely predicts that optimizing nutrient-sensing molecular signaling in adulthood can simultaneously postpone ageing and increase Darwinian fitness. Read More

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http://dx.doi.org/10.1002/evl3.108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457396PMC

Exosomes from N-Myc amplified neuroblastoma cells induce migration and confer chemoresistance to non-N-Myc amplified cells: implications of intra-tumour heterogeneity.

J Extracell Vesicles 2019 11;8(1):1597614. Epub 2019 Apr 11.

Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.

Neuroblastoma accounts for 15% of childhood cancer mortality. Amplification of the oncogene N-Myc is a well-established poor prognostic marker for neuroblastoma. Whilst N-Myc amplification status strongly correlates with higher tumour aggression and resistance to treatment, the role of N-Myc in the aggressiveness of the disease is poorly understood. Read More

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http://dx.doi.org/10.1080/20013078.2019.1597614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461098PMC

Treatment According to Molecular Profiling in Relapsed/Refractory Cancer Patients: A Review Focusing on Latest Profiling Studies.

Comput Struct Biotechnol J 2019 26;17:447-453. Epub 2019 Mar 26.

Department of Haematology and Oncology, Medical University of Innsbruck, Austria.

In this review we aim to summarize studies investigating the impact of a molecular profiling (MP)-guided treatment approach in heavily pretreated cancer patients. In summary, many independent single- and multicenter studies showed a significant benefit of MP-guided treatment regarding response rates and survival. However, in the only randomized trial conducted so far, no benefit of MP-guided targeted therapy was observed. Read More

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http://dx.doi.org/10.1016/j.csbj.2019.03.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453774PMC

Biomedical applications of mRNA nanomedicine.

Nano Res 2018 Oct 27;11(10):5281-5309. Epub 2018 Jul 27.

Center for Nanomedicine and Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

As an attractive alternative to plasmid DNA, messenger RNA (mRNA) has recently emerged as a promising class of nucleic acid therapeutics for biomedical applications. Advances in addressing the inherent shortcomings of mRNA and in the development of nanoparticle-based delivery systems have prompted the development and clinical translation of mRNA-based medicines. In this review, we discuss the chemical modification strategies of mRNA to improve its stability, minimize immune responses, and enhance translational efficacy. Read More

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http://dx.doi.org/10.1007/s12274-018-2146-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472920PMC
October 2018

BAYESIAN LATENT HIERARCHICAL MODEL FOR TRANSCRIPTOMIC META-ANALYSIS TO DETECT BIOMARKERS WITH CLUSTERED META-PATTERNS OF DIFFERENTIAL EXPRESSION SIGNALS.

Ann Appl Stat 2019 Mar 10;13(1):340-366. Epub 2019 Apr 10.

Department of Biostatistics, Human Genetics and Computational Biology University of Pittsburgh Pittsburgh, PA 15261

Due to the rapid development of high-throughput experimental techniques and fast-dropping prices, many transcriptomic datasets have been generated and accumulated in the public domain. Meta-analysis combining multiple transcriptomic studies can increase the statistical power to detect disease-related biomarkers. In this paper, we introduce a Bayesian latent hierarchical model to perform transcriptomic meta-analysis. Read More

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http://dx.doi.org/10.1214/18-AOAS1188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472949PMC

Downregulation of lncRNA BANCR participates in the development of retinopathy among diabetic patients.

Exp Ther Med 2019 May 26;17(5):4132-4138. Epub 2019 Mar 26.

Department of Ophthalmology, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, P.R. China.

The long non-coding (lnc)RNA B-Raf proto-oncogene, serine/threonine kinase-activated non-protein coding RNA (BANCR) is a well-characterized oncogene, while its potential functions in other diseases remain elusive. In the present study, the possible association of BANCR with diabetic retinopathy was investigated. A total of 244 patients with diabetes were followed up every 6 months for 8 years to record the occurrence of retinopathy. Read More

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http://dx.doi.org/10.3892/etm.2019.7444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468933PMC

Epigenetic Influences in the Obesity/Colorectal Cancer Axis: A Novel Theragnostic Avenue.

J Oncol 2019 17;2019:7406078. Epub 2019 Mar 17.

Unidad de Gestión Clínica de Endocrinología y Nutrición del Hospital Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga (IBIMA), Universidad de Málaga, Málaga, Spain.

The World Health Organization (WHO) considers that obesity has reached proportions of pandemic. Experts also insist on the importance of considering obesity as a chronic disease and one of the main contributors to the worldwide burden of other nontransmissible chronic diseases, which have a great impact on health, lifestyle, and economic cost. One of the most current challenges of biomedical science faces is to understand the origin of the chronic nontransmissible diseases, such as obesity and cancer. Read More

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http://dx.doi.org/10.1155/2019/7406078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441533PMC

Development of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of .

Plant Pathol J 2019 Apr 1;35(2):164-171. Epub 2019 Apr 1.

Department of Plant Science, Seoul National University, Seoul 08826, Korea.

An assay for detecting (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with real-time detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of 1 × 10 to 1 × 10 ASSVd RNA copies/μl with reproducibility and precision. Read More

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http://dx.doi.org/10.5423/PPJ.OA.10.2018.0206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6464194PMC
April 2019
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