1,018,089 results match your criteria RNA New York N.Y.[Journal]


Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus.

Nucleic Acids Res 2018 Dec 15. Epub 2018 Dec 15.

Université de Rennes 1, Inserm, BRM (Bacterial Regulatory RNAs and Medicine) UMR_S 1230, 35000 Rennes, France.

Toxin-antitoxin (TA) systems are ubiquitous among bacteria, frequently expressed in multiple copies, and important for functions such as antibiotic resistance and persistence. Type I TA systems are composed of a stable toxic peptide whose expression is repressed by an unstable RNA antitoxin. Here, we investigated the functionalities, regulation, and possible cross-talk between three core genome copies of the pathogenicity island-encoded 'sprG1/sprF1' type I TA system in the human pathogen Staphylococcus aureus. Read More

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December 2018

Synthesis of a new disulfide Fmoc monomer for creating biologically susceptible linkages in peptide nucleic acid oligomers.

Bioorg Chem 2018 Dec 1;84:394-398. Epub 2018 Dec 1.

Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA. Electronic address:

Peptide nucleic acids (PNA) are one of many synthetic mimics of DNA and RNA that have found applications as biological probes, as nano-scaffold components, and in diagnostics. In an effort to use PNA as constructs for cellular delivery we investigated the possibility of installing a biologically susceptible disulfide bond in the backbone of a PNA oligomer. Here we report the synthesis of a new abasic Fmoc monomer containing a disulfide bond that can be incorporated into a PNA oligomer (DS-PNA) using standard solid phase peptide synthesis. Read More

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December 2018

Fish antiviral tripartite motif (TRIM) proteins.

Fish Shellfish Immunol 2018 Dec 11. Epub 2018 Dec 11.

INRA, Virologie et Immunologie Moléculaires, Université Paris-Saclay, Jouy-en-Josas, France. Electronic address:

Tripartite motif (TRIM) family or RBCC proteins comprises characteristic zinc-binding domains (a RING (R), a B-box type 1 (B1) and a B-box type 2 (B2)) and coiled-coil (CC) domain followed by a C-terminus variable domain. There are about 80 different TRIM proteins in human, but more than 200 in zebrafish with several large gene expansions (ftr >70 genes; btr >30 genes; trim35 > 30 genes). Repertoires of trim genes in fish are variable across fishes, but they have been remarkably diversified independently in a number of species. Read More

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December 2018

Three monophyletic clusters in Retortamonas species isolated from vertebrates.

Parasitol Int 2018 Dec 11. Epub 2018 Dec 11.

Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan. Electronic address:

Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. Read More

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December 2018
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CircRNAs and cancer: biomarkers and master regulators.

Semin Cancer Biol 2018 Dec 11. Epub 2018 Dec 11.

Molecular Oncology group, Biodonostia Research Institute, Paseo Doctor Begiristain, s/n San Sebastián, 20014, Spain; Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom; IKERBASQUE, Basque Foundation for Science, María Díaz Haroko Kalea, 3, 48013, Bilbao, Spain. Electronic address:

Circular RNAs (circRNAs) are a novel class of regulatory RNAs that despite being relatively abundant have only recently begun to be explored. There are many thousands of genes that appear capable of producing circRNAs, however the function of all but a handful remain to be determined. What is emerging about these highly conserved molecules is that they play important roles in biology and cancer biology in particular. Read More

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December 2018
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LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Orthopedics, Affiliated Hospital of Jining Medical University, Jining 272029, China. Electronic address:

Aims: PMS1 Homolog 2, Mismatch Repair System Component Pseudogene 2 (PMS2L2) has been reported as an up-regulated long non-coding RNA (lncRNA) in osteoarthritis (OA) tissues. The purpose of the present work is to explore whether the differently expressed PMS2L2 is associated with the pathogenesis of OA.

Main Methods: Chondrogenic ATDC5 cells were exposed to various doses of lipopolysaccharide (LPS). Read More

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December 2018

Expanding the Molecular Characterization of Thoracic Inflammatory Myofibroblastic Tumors beyond ALK gene rearrangements.

J Thorac Oncol 2018 Dec 11. Epub 2018 Dec 11.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY. Electronic address:

Background: Half of inflammatory myofibroblastic tumors (IMTs) regardless of anatomic location harbor ALK gene rearrangements and overexpress ALK protein. The wide application of next generation sequencing (NGS) and the clinical benefit to tyrosine kinase inhibitors have opened new opportunities for investigation of ALK-negative IMT.

Design: In this study we investigate a series of pediatric and adult thoracic IMT for abnormalities in a wide spectrum of actionable kinases, by applying a variety of molecular and NGS techniques, including FISH, targeted RNA sequencing and NanoString. Read More

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December 2018
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TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR.

Biochimie 2018 Dec 11. Epub 2018 Dec 11.

Respiratory Medicine, Hunan Provincial People's Hospital, No. 61 Jiefang Xi Road, Changsha, 410219, Hunan, PR China; Institute of Respiratory Medicine, Changsha Medical College, No. 1501 Leifeng Road, Changsha, 410219, Hunan, PR China. Electronic address:

The present study was designed to explore the role of transient receptor potential canonical 3 (TRPC3) in nicotine-induced chronic obstructive pulmonary disease (COPD) and its underlying mechanism. In this study, the expression and localization of α5 nicotinic acetylcholine receptor (α5-nAchR) in lung tissues were determined by western blotting and immunohistochemistry. The quantitative real-time PCR (qRT-PCR) analysis was performed to examine the mRNA expression levels of α5-nAchR and TRPC3 in human airway smooth muscle cells (HASMCs). Read More

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December 2018

Neutrophils Restrict Tumor-Associated Microbiota to Reduce Growth and Invasion of Colon Tumors in Mice.

Gastroenterology 2018 Dec 11. Epub 2018 Dec 11.

Molecular & Integrative Physiology, University of Michigan School of Public Health, Ann Arbor MI; Internal Medicine Division of Gastroenterology, University of Michigan School of Public Health, Ann Arbor MI; Rogel Cancer Center. University of Michigan Medical School, Ann Arbor MI. Electronic address:

Background & Aims: Neutrophils are among the most prevalent immune cells in the microenvironment of colon tumors, are believed to promote growth of colon tumors, and their numbers correlate with outcomes of patients with colon cancer. Trials of inhibitors of neutrophil trafficking are under way in patients with cancer, but it is not clear how neutrophils contribute to colon tumorigenesis.

Methods: Colitis-associated colon cancer was induced mice with conditional deletion of neutrophils (LysMCre;Mcl1) and wild-type littermates (LysMCre;Mcl1, control mice) by administration of azoxythmethane and/or dextran sulfate sodium. Read More

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December 2018

Virus and host interactions critical for filoviral RNA synthesis as therapeutic targets.

Antiviral Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Filoviruses, which include Ebola virus and Marburg virus, are negative-sense RNA viruses associated with sporadic outbreaks of severe viral hemorrhagic fever characterized by uncontrolled virus replication. The extreme virulence and emerging nature of these zoonotic pathogens make them a significant threat to human health. Replication of the filovirus genome and production of viral RNAs requires the function of a complex of four viral proteins, the nucleoprotein (NP), viral protein 35 (VP35), viral protein 30 (VP30) and large protein (L). Read More

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December 2018

Organoid Modeling of the Tumor Immune Microenvironment.

Cell 2018 Dec;175(7):1972-1988.e16

Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA, USA. Electronic address:

In vitro cancer cultures, including three-dimensional organoids, typically contain exclusively neoplastic epithelium but require artificial reconstitution to recapitulate the tumor microenvironment (TME). The co-culture of primary tumor epithelia with endogenous, syngeneic tumor-infiltrating lymphocytes (TILs) as a cohesive unit has been particularly elusive. Here, an air-liquid interface (ALI) method propagated patient-derived organoids (PDOs) from >100 human biopsies or mouse tumors in syngeneic immunocompetent hosts as tumor epithelia with native embedded immune cells (T, B, NK, macrophages). Read More

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December 2018
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Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication.

Cell 2018 Dec;175(7):1917-1930.e13

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94158, USA; J. David Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address:

Ebola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-protein interaction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulator VP30 and the host ubiquitin ligase RBBP6. Read More

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December 2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Cell 2018 Dec;175(7):1887-1901.e18

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China; University of Chinese Academy of Sciences, 100049 Beijing, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 100101 Beijing, China. Electronic address:

In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically expressed in the nucleus of two- to four-cell mouse embryos. Overexpression of LincGET in one of the two-cell blastomeres biases its progeny predominantly toward the inner cell mass (ICM) fate. Read More

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December 2018
2 Reads

The role of RNA adenosine demethylases in the control of gene expression.

Biochim Biophys Acta Gene Regul Mech 2018 Dec 11. Epub 2018 Dec 11.

Central European Institute of Technology (CEITEC), Masaryk University, Brno 625 00, Czech Republic. Electronic address:

RNA modifications are being recognized as an essential factor in gene expression regulation. They play essential roles in germ line development, differentiation and disease. In eukaryotic mRNAs, N-adenosine methylation (mA) is the most prevalent internal chemical modification identified to date. Read More

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December 2018

m6A modification of non-coding RNA and the control of mammalian gene expression.

Biochim Biophys Acta Gene Regul Mech 2018 Dec 11. Epub 2018 Dec 11.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

The biology of non-coding RNA (ncRNA) and the regulation of mammalian gene expression is a rapidly expanding field. In this review, we consider how recent advances in technology, enabling the precise mapping of modifications to RNA transcripts, has provided new opportunities to dissect post-transcriptional gene regulation. With this has come the realisation that in the absence of translation, the modification of ncRNAs may play a fundamental role in their regulation, protein interactome and subsequent downstream effector functions. Read More

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December 2018

Interaction of Phthorimaea operculella granulovirus with a Nosema sp. microsporidium in larvae of Phthorimaea operculella.

J Invertebr Pathol 2018 Dec 11. Epub 2018 Dec 11.

Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute, Heinrichstraße 243, 64287 Darmstadt, Germany. Electronic address:

An antagonistic effect of a microsporidium (Nosema sp.) infection on the virulence of Phthorimaea operculella granulovirus (PhopGV) was recorded in potato tuber moth (Phthorimaea operculella) larvae with mixed infections. When the P. Read More

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December 2018

Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8.

PLoS One 2018 14;13(12):e0207863. Epub 2018 Dec 14.

Embrapa Agrobiologia, Seropédica-RJ-CEP, Brazil.

The stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. Read More

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December 2018

S-MiRAGE: A quantitative, secreted RNA-based reporter of gene expression and cell persistence.

ACS Synth Biol 2018 Dec 14. Epub 2018 Dec 14.

Non-destructive measurements of cell persistence and gene expression are crucial for longitudinal research studies and for prognostic assessment of cell therapies. Here we describe S-MiRAGE, a platform that utilizes small secreted RNA molecules as sensitive and quantitatively accurate reporters of cellular processes. S-MiRAGE allows cellular numbers or gene expression to be measured from culture media or from biofluids. Read More

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December 2018

Effect of lncRNA BNC2-AS1 on the Proliferation, Migration and Invasion of Gastric Cancer Cells.

Clin Lab 2018 Oct;64(11)

Background: Gastric cancer is one of the most common cancers and is the second leading cause of cancer mortality worldwide. The present study aimed to investigate the potential biological effect of long non-coding RNA (lncRNA) BNC2-AS1 on the proliferation, migration, and invasion of cervical cancer cells. Methods: BNC2-AS1 small interfering RNA (siRNA) was transfected into SGC7901 and BGC823 gastric cancer cell lines, with negative siRNA serving as a control. Read More

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October 2018
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Long Non-Coding RNA TUG1 as a Potential Novel Biomarker for Predicting the Clinical Outcome of Cancer Patients: a Meta-Analysis.

Clin Lab 2018 Oct;64(11)

Background: Emerging evidence suggests that long non-coding RNAs (lncRNAs) can be used as potential biomarkers for a wide range of cancers. Herein, the prognostic value of lncRNA taurine upregulated gene (TUG1) was analyzed using meta-analysis in different kinds of cancers. Methods: Databases including Cochrane Library, PubMed, and Chinese National Knowledge Infrastructure were searched for epidemiological studies up to March 2018. Read More

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October 2018

An integrated enrichment system to facilitate isolation and molecular characterization of single cancer cells from whole blood.

Cytometry A 2018 Dec;93(12):1226-1233

Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

Circulating tumor cells (CTCs) carry valuable biological information. While enumeration of CTCs in peripheral blood is an FDA-approved prognostic indicator of survival in metastatic prostate and other cancers, analysis of CTC phenotypic and genomic markers is needed to identify cancer origin and elucidate pathways that can guide therapeutic selection for personalized medicine. Given the emergence of single-cell mRNA sequencing technologies, a method is needed to isolate CTCs with high sensitivity and specificity as well as compatibility with downstream genomic analysis. Read More

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December 2018

Injecting RNA interference lentiviruses targeting the muscarinic 3 receptor gene into the bladder wall inhibits neurogenic detrusor overactivity in rats with spinal cord injury.

Neurourol Urodyn 2018 Dec 14. Epub 2018 Dec 14.

Department of Urology, Xuanwu Hospital Capital Medical University, Xuanwu District, Beijing, China.

Aims: To investigate the effects of injecting RNA interference (RNAi) lentiviruses targeting the muscarinic 3 (M ) receptor gene into the bladder wall on bladder activity in rats with spinal cord injury (SCI).

Methods: Four M RNAi lentiviruses were constructed and used to infect primary cultured bladder smooth muscle cells (BSMCs). Western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were performed to determine the optimal RNAi lentivirus with the highest interference efficiency. Read More

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December 2018

Identification of potential prognostic TF-associated lncRNAs for predicting survival in ovarian cancer.

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

The First Affiliated Hospital, Harbin Medical University, Harbin, China.

The pathophysiology of ovarian cancer (OV) is complex and depends on multiple biological processes and pathways. Therefore, there is an urgent need to identify reliable prognostic biomarkers for predicting clinical outcomes and helping personalize treatment of OV. A long non-coding RNA (lncRNA)-based risk score model was constructed to infer the prognostic efficacy of transcription factors (TFs) based on the OV dataset from The Cancer Genome Atlas. Read More

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December 2018

xCas9 expands the scope of genome editing with reduced efficiency in rice.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

CRISPR-Cas9 systems have been widely used in animals and plants for genome editing, epigenetic modification, etc. (Cong et al., 2013, Hilton et al. Read More

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December 2018
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Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening.

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

Department of Human Genetics, University Medicine Greifswald and Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald, Greifswald, Germany.

CCM3, originally described as PDCD10, regulates blood-brain barrier integrity and vascular maturation in vivo. CCM3 loss-of-function variants predispose to cerebral cavernous malformations (CCM). Using CRISPR/Cas9 genome editing, we here present a model which mimics complete CCM3 inactivation in cavernous endothelial cells (ECs) of heterozygous mutation carriers. Read More

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December 2018

Mineralocorticoid receptor deficiency improves the therapeutic effects of mesenchymal stem cells for myocardial infarction via enhanced cell survival.

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

The poor survival of stem cells seriously limits their therapeutic efficacy for myocardial infarction (MI). Mineralocorticoid receptor (MR) activation plays an important role in the pathogenesis of multiple cardiovascular diseases. Here, we examined whether MR silencing in bone marrow derived mesenchymal stem cells (MSCs) could improve MSCs' survival and enhance their cardioprotective effects in MI. Read More

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December 2018

Epstein-Barr virus and human papillomaviruses as favorable prognostic factors in nasopharyngeal carcinoma: A nationwide study in Finland.

Head Neck 2018 Dec 14. Epub 2018 Dec 14.

Department of Pathology, University of Turku and Turku University Hospital, Turku, Finland.

Background: Nasopharyngeal carcinoma (NPC) is related to Epstein-Barr virus (EBV) in endemic areas; however, the role of viruses in nonendemic countries is unclear. Our nationwide study investigated the prevalence and prognostic significance of EBV and human papillomaviruses (HPVs) in Finnish NPC tumors.

Methods: We analyzed samples from 150 patients diagnosed between 1990 and 2009. Read More

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December 2018

EgRBP42 from oil palm enhances adaptation to stress in Arabidopsis through regulation of nucleocytoplasmic transport of stress-responsive mRNAs.

Plant Cell Environ 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Abiotic stress reduces plant growth and crop productivity. However, the mechanism underlying post-transcriptional regulations of stress response remains elusive. Herein, we report the post-transcriptional mechanism of nucleocytoplasmic RNA transport of stress-responsive transcripts mediated by EgRBP42, an hnRNP-like RNA-binding protein from oil palm, which could be necessary for rapid protein translation to confer abiotic stress tolerance in plants. Read More

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December 2018

LncRNA NEAT1 promotes extracellular matrix accumulation and epithelial-to-mesenchymal transition by targeting miR-27b-3p and ZEB1 in diabetic nephropathy.

J Cell Physiol 2018 Dec 13. Epub 2018 Dec 13.

Department of Nephrology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, People's Republic of China.

Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. Read More

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December 2018

Knockdown of DGCR5 enhances the radiosensitivity of human laryngeal carcinoma cells via inducing miR-195.

J Cell Physiol 2018 Dec 13. Epub 2018 Dec 13.

Department of Oncology, RenMin Hospital of Wuhan University, Wuhan, China.

Long noncoding RNAs (lncRNAs) exert critical roles in the development of various cancers, including human laryngeal cancer. Radioresistance contributes to the predominant causes of laryngeal cancer recurrence after radiotherapy. The aim of our study was to investigate the association of dysregulated lncRNA and radiation resistance in human larynx squamous carcinoma. Read More

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December 2018

Genome-Wide Maps of Transcription Regulatory Elements and Transcription Enhancers in Development and Disease.

Compr Physiol 2018 Dec 13;9(1):439-455. Epub 2018 Dec 13.

Blood Research Institute, BloodCenter of Wisconsin, a part of Versiti, Milwaukee, Wisconsin, USA.

Gene expression is regulated by numerous elements including enhancers, insulators, transcription factors, and architectural proteins. Regions of DNA distal to the transcriptional start site, called enhancers, play a central role in the temporal and tissue-specific regulation of gene expression through RNA polymerase II. The identification of enhancers and other cis regulatory elements has largely been possible due to advances in next generation sequencing technologies. Read More

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December 2018
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Molecular mechanisms of plant acclimation to changing environments.

Plant Biol (Stuttg) 2019 Jan;21 Suppl 1:3-5

Center of Excellence on Plant Sciences (CEPLAS), Institute of Plant Biochemistry Heinrich-Heine-University, Düsseldorf, Germany.

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January 2019

Development of an HIV-1 Integrase Genotyping Assay for HIV-1 Subtype AE.

J Med Virol 2018 Dec 13. Epub 2018 Dec 13.

Gilead Sciences, Inc., Foster City, CA, USA.

An HIV-1 integrase (IN) genotyping assay was developed to evaluate clinical samples from patients infected with HIV-1 subtype AE, a subtype highly prevalent in Asia. The HIV-1 integrase gene was amplified from plasma-derived HIV-1 viral RNA via RT-PCR followed by population sequencing. Assay sensitivity was also evaluated using serially diluted plasma samples. Read More

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December 2018

Hepatitis E virus blood donor NAT screening: as much as possible or as much as needed?

Transfusion 2018 Dec 13. Epub 2018 Dec 13.

Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein- Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany.

Background: The cost-benefit question of general screening of blood products for the hepatitis E virus (HEV) is currently being discussed. One central question is the need for individual nucleic acid amplification techniques (NAT) screening (ID-NAT) versus minipool NAT screening (MP-NAT) approaches to identify all relevant viremias in blood donors. Here, the findings of ID-NAT versus MP-NAT in pools of 96 samples were compared. Read More

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December 2018

Inhibition of Hepatitis C replication by targeting the molecular chaperone Hsp90 - synthesis and biological evaluation of 4,5,6,7-tetrahydrobenzo[1,2-d]thiazole derivatives.

ChemMedChem 2018 Dec 13. Epub 2018 Dec 13.

University of Helsinki, Centre for Drug Research, Division of Pharmaceutical Biosciences, Viikinkaari 4, 00014, Helsinki, FINLAND.

Cellular chaperones that belong to the heat-shock protein 90 (Hsp90) family are a prerequisite for successful viral propagation for most viruses. The hepatitis C virus (HCV) uses Hsp90 for maturation, folding and modification of viral proteins. Based on our previous discovery that marine alkaloid analogues with 4,5,6,7-tetrahydrobenzo[1,2-d]thiazole-2-amine structure show inhibition of HCV replication and potential binding to Hsp90, a series of twelve novel compounds based on this scaffold was designed and synthesized. Read More

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December 2018

Systemic disrupting the homeostasis of tRNA isopentenyltransferase causes growth and development abnormalities in Bombyx mori.

Insect Mol Biol 2018 Dec 12. Epub 2018 Dec 12.

State Key Laboratory of Silkworm Genome Biology, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, China.

Isopentenylation at A37 (i A37) of some transfer RNAs (tRNAs) plays a vital role in regulating the efficiency and fidelity of protein synthesis. However, whether insects, which are well-known for their highly efficient protein synthesis machinery, employ this regulatory mechanism remains uninvestigated. In the current study, a candidate tRNA isopentenyltransferase (IPT) gene with three alternative splicing isoforms (BmIPT1-BmIPT3) was identified in Bombyx mori (silkworm). Read More

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December 2018

Kernel size-related genes revealed by an integrated eQTL analysis during early maize kernel development.

Plant J 2018 Dec 12. Epub 2018 Dec 12.

Biotechnology Research Institute.

In maize, kernel traits strongly impact overall grain yields, and it is known that sophisticated spatiotemporal programs of gene expression coordinate kernel development, so advancing our knowledge of kernel development can help efforts to improve grain yields. Here, using phenotype, genotype, and transcriptomics data of maize kernels at 5 and 15 days after pollination (DAP) for a large association mapping panel, we employed multiple quantitative genetics approaches-genome wide association studies (GWAS) as well as expression quantitative trait loci (eQTL) and quantitative trait transcript (QTT) analyses-to gain insights about molecular genetic basis of kernel development in maize. This resulted in the identification of 137 putative kernel length-related genes at 5 DAP, of which 43 are located in previously reported QTL regions. Read More

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December 2018
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Uptake of direct acting antiviral therapies for the treatment of hepatitis C virus among people who inject drugs in a universal health-care system.

Drug Alcohol Rev 2018 Dec 12. Epub 2018 Dec 12.

National Drug and Alcohol Research Centre, University of New South Wales, Sydney, Australia.

Introduction And Aims: Direct acting antiviral (DAA) treatment can reduce hepatitis C virus (HCV) infection incidence and mortality, although large scale uptake of these treatments is necessary to achieve those reductions. Targeting people who inject drugs (PWID) will be crucial to achieve the necessary reductions. Previously, treatment uptake has been very low (1-2%) among PWID, but these new DAAs have been subsidised in Australia since March 2016. Read More

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December 2018

Overexpressed circ_0029426 in glioblastoma forecasts unfavorable prognosis and promotes cell progression by sponging miR-197.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Cerebrovascular Disease, Henan Provincial People's Hospital, Zhengzhou, China.

Accumulating studies indicates that circular RNAs (circRNAs) play an imperative role in modulating cancer progression and metastasis. In the previous study, elevated circ_0029426 was first observed in glioblastoma (GBM) tissues compared with normal tissues by circRNA microarray. Our aim is to study the function and mechanism of circ_0029426 in GBM. Read More

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December 2018
2 Reads

Multiple imputation approach for interval-censored time to HIV RNA viral rebound within a mixed effects Cox model.

Biom J 2018 Dec 13. Epub 2018 Dec 13.

Departarment d'Estadística i Investigació Operativa, Universitat Politècnica de Catalunya/BARCELONATECH, Barcelona, Spain.

We present a method to fit a mixed effects Cox model with interval-censored data. Our proposal is based on a multiple imputation approach that uses the truncated Weibull distribution to replace the interval-censored data by imputed survival times and then uses established mixed effects Cox methods for right-censored data. Interval-censored data were encountered in a database corresponding to a recompilation of retrospective data from eight analytical treatment interruption (ATI) studies in 158 human immunodeficiency virus (HIV) positive combination antiretroviral treatment (cART) suppressed individuals. Read More

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December 2018

Effect of STOX1 on recurrent spontaneous abortion by regulating trophoblast cell proliferation and migration via the PI3K/AKT signaling pathway.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China.

STOX1 is a transcription factor that is implicated in the high prevalence of human gestational diseases. It has been studied in various types of gestational diseases using different molecular and cellular biological technologies. However, the effect and detailed mechanism of storkhead box 1 (STOX1) in recurrent spontaneous abortion (RSA) remain unknown. Read More

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December 2018

MicroRNA-384 regulates cell proliferation and apoptosis through directly targeting WISP1 in laryngeal cancer.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Laryngeal cancer (LC) is an increasingly common malignant tumors of head and neck cancer. Aberrant expression of microRNA (miRNA) is closely related with LC development. In the current study, we investigated the biological function and underlying molecular mechanism of miR-384 in LC. Read More

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December 2018

The effect of early growth response 1 on levels of Amyloid-β 40 peptide in U87MG cells.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Neurology, The Frist Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

A recent study has shown that early growth response 1 (EGR1) plays a critical role in the β-amyloid cascade and tau hypotheses. In addition, evidence has suggested that EGR1 can regulate levels of amyloid-beta peptides, key molecules in the pathogenesis of Alzheimer's disease (AD). However, whether EGR1 is a deleterious or protective factor in the AD is still controversial. Read More

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December 2018

Clinical and molecular characteristics of bladder urothelial carcinoma subtypes.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Organization, General Hospital of Benxi Iron and Steel Co., Benxi, China.

Bladder urothelial carcinoma (BLCA) is a common malignancy with high heterogeneity. A reasonable molecular subtyping can facilitate biological study and personalized therapy of BLCA. In this study, unsupervised consensus clustering was used to acquire the molecular subtypes of BLCA based on messenger RNA (mRNA) and microRNA (miRNA) data. Read More

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December 2018
2 Reads

Methylene blue regulates inflammatory response in osteoarthritis by noncoding long chain RNA CILinc02.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

The Second Department of Orthopedics, Zhang Zhou Zhengxing Hospital, Zhangzhou, China.

Long noncoding RNAs serve as novel regulatory molecules involved in various biological processes, but their role in osteoarthritis (OA) remains unknown. Therefore, we aimed to reveal the inflammatory mechanisms regulated by CILinc02 and methylene blue in human osteoarthritic cartilage and to explore the potential functions of CILinc02 and methylene blue in OA. The expression level of CILinc02 in OA cartilage tissues and primary cells was obtained using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Read More

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December 2018
1 Read

Long noncoding RNA CCAT2 functions as a competitive endogenous RNA to regulate FOXC1 expression by sponging miR-23b-5p in lung adenocarcinoma.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Thoracic Surgery, Huai'an Second People's Hospital and The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.

Long noncoding RNA (lncRNA) may regulate the process of tumor formation. Although lncRNA CCAT2 has been identified as a key point in many diseases, its pathophysiological mechanism in lung adenocarcinoma remains unknown. We measured the expression level of CCAT2 in lung adenocarcinoma cells and normal lung epithelial cell line BEAS-2B by quantitative real-time polymerase chain reaction (qRT-PCR). Read More

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December 2018

Knockdown of FOXO6 inhibits high glucose-induced oxidative stress and apoptosis in retinal pigment epithelial cells.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Oxidative stress and apoptosis in retinal pigment epithelium cells are involved in the pathogenesis of diabetic retinopathy (DR). Forkhead box class O 6 (FOXO6) is a member of the FOXO family that can regulate diabetes-induced oxidative stress. However, the role of FOXO6 in DR has not been clarified. Read More

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December 2018

Bile salt (glycochenodeoxycholate acid) induces cell survival and chemoresistance in hepatocellular carcinoma.

J Cell Physiol 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Nanobiological Technology of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Objective: Glycochenodeoxycholate acid (GCDA) is a toxic component in bile salts. It plays an important role in the development and progression of liver cancer. In this study, we investigated the underlying mechanism of GCDA in hepatocarcinogenesis and chemotherapy resistance. Read More

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December 2018

Tissue-based transcriptomics of Chionodraco hamatus: sequencing, de novo assembly, annotation and marker discovery.

J Fish Biol 2018 Dec 13. Epub 2018 Dec 13.

Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.

Here, we explored the liver, heart and muscle tissue transcriptome of the haemoglobinless Antarctic fish Chionodraco hamatus using the Illumina paired-end RNA sequencing. A total of 114,028 unigenes with a mean length of 794.24 bp was produced. Read More

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December 2018

Molecular signatures of increased freezing tolerance due to low temperature memory in Arabidopsis.

Plant Cell Environ 2018 Dec 12. Epub 2018 Dec 12.

Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam, Germany.

Alternating temperatures require fast and coordinated adaptation responses of plants. Cold acclimation has been extensively investigated and results in increased freezing tolerance in Arabidopsis thaliana. Here we show that the two Arabidopsis accessions Col-0 and N14 that differ in their freezing tolerance, showed memory of cold acclimation, i. Read More

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December 2018
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