205 results match your criteria Pure B-Cell Disorders


SLy2-overexpression impairs B-cell development in the bone marrow and the IgG response towards pneumococcal conjugate-vaccine.

Immun Inflamm Dis 2021 Feb 16. Epub 2021 Feb 16.

Department of Pharmacology, Experimental Therapy and Toxicology, Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA, University of Tuebingen, Tuebingen, Germany.

Background: Infections with Streptococcus pneumoniae can cause severe diseases in humans including pneumonia. Although guidelines for vaccination have been established, S. pneumoniae is still responsible for a serious burden of disease around the globe. Read More

View Article and Full-Text PDF
February 2021

Thymic Hyperplasia with Lymphoepithelial Sialadenitis (LESA)-Like Features: Strong Association with Lymphomas and Non-Myasthenic Autoimmune Diseases.

Cancers (Basel) 2021 Jan 16;13(2). Epub 2021 Jan 16.

Institute of Pathology, University Medical Centre Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.

Thymic hyperplasia (TH) with lymphoepithelial sialadenitis (LESA)-like features (LESA-like TH) has been described as a tumor-like, benign proliferation of thymic epithelial cells and lymphoid follicles. We aimed to determine the frequency of lymphoma and autoimmunity in LESA-like TH and performed retrospective analysis of cases with LESA-like TH and/or thymic MALT-lymphoma. Among 36 patients (21 males) with LESA-like TH (age 52 years, 32-80; lesion diameter 7. Read More

View Article and Full-Text PDF
January 2021

Anti-carbamylated protein antibody isotype pattern differs between palindromic rheumatism and rheumatoid arthritis.

Ther Adv Musculoskelet Dis 2020 10;12:1759720X20978139. Epub 2020 Dec 10.

Arthritis Unit, Rheumatology Department, Hospital Clinic of Barcelona, Villarroel 170, Barcelona, 08036, Spain.

Background: A restricted response against citrullinated peptides/proteins, with less isotype usage, has been found in palindromic rheumatism (PR) in comparison with rheumatoid arthritis (RA). We hypothesized that this different antibody response may be observed for other post-translational modified proteins. We compared the prevalence and isotype usage of two specificities of anti-carbamylated peptide/protein antibodies (Anti-CarP) in patients with PR and RA. Read More

View Article and Full-Text PDF
December 2020

Advanced imaging for quantification of abnormalities in the salivary glands of patients with primary Sjögren's syndrome.

Rheumatology (Oxford) 2020 Nov 21. Epub 2020 Nov 21.

Research and Development, GlaxoSmithKline, Stevenage.

Objectives: To assess non-invasive imaging for detection and quantification of gland structure, inflammation and function in patients with primary Sjogren's syndrome (pSS) using PET-CT with 11C-Methionine (11C-MET; radiolabelled amino acid), and 18F-fluorodeoxyglucose (18F-FDG; glucose uptake marker), to assess protein synthesis and inflammation, respectively; multiparametric MRI evaluated salivary gland structural and physiological changes.

Methods: In this imaging/clinical/histology comparative study (GSK study 203818; NCT02899377) patients with pSS and age- and sex-matched healthy volunteers underwent MRI of the salivary glands and 11C-MET PET-CT. Patients also underwent 18F-FDG PET-CT and labial salivary gland biopsies. Read More

View Article and Full-Text PDF
November 2020

Exploration of surface glycoprotein to design multi-epitope vaccine for the prevention of Covid-19.

Inform Med Unlocked 2020 4;21:100438. Epub 2020 Oct 4.

Department of Natural Sciences, Precious Conerstone University, Ibadan, Oyo State, Nigeria.

Stimulation and generation of T and B cell-mediated long-term immune response are essential for the curbing of a deadly virus such as SAR-CoV-2 (Severe Acute Respiratory Corona Virus 2). Immunoinformatics approach in vaccine design takes advantage of antigenic and non-allergenic epitopes present on the spike glycoprotein of SARS-CoV-2 to elicit immune responses. T cells and B cells epitopes were predicted, and the selected residues were subjected to allergenicity, antigenicity and toxicity screening which were linked by appropriate linkers to form a multi-epitope subunit vaccine. Read More

View Article and Full-Text PDF
October 2020

Dysregulation in B-cell responses and T follicular helper cell function in ADA2 deficiency patients.

Eur J Immunol 2021 01 28;51(1):206-219. Epub 2020 Aug 28.

Unità Operativa Semplice Dipartimentale Centro Malattie Autoinfiammatorie e Immunodeficienze, IRCCS Istituto Giannina Gaslini, Genova, Italy.

Adenosine deaminase 2 deficiency (DADA2) is an autoinflammatory disease characterized by inflammatory vasculopathy, early strokes associated often with hypogammaglobulinemia. Pure red cell aplasia, thrombocytopenia, and neutropenia have been reported. The defect is due to biallelic loss of function of ADA2 gene, coding for a protein known to regulate the catabolism of extracellular adenosine. Read More

View Article and Full-Text PDF
January 2021

Psychiatric Disturbance or Parkinsonism as a Presentation of CNS Lymphoma: Observational Retrospective Study and Review of Literature.

Am J Clin Oncol 2020 10;43(10):727-733

Departments of Neurology.

Objective: To evaluate the incidence of and characterize the presentation of neuropsychiatric symptoms and/or Parkinsonism as a presentation of central nervous system lymphoma (CNSL) in either its primary CNSL form or when it spreads to the brain in systemic diffuse large B-cell lymphoma (secondary CNSL).

Patients And Methods: With Institutional Review Board approval we identified patients who had been treated at Mayo Clinic from 1998 to 2018 and were recorded to have a combination of ICD 9/10 codes for CNSL and various psychiatric diagnoses.

Results: A total of 20 of the 232 patients (9%) were noted to have neuropsychiatric symptoms preceding diagnosis. Read More

View Article and Full-Text PDF
October 2020

Intrasinusoidal HHV8-EBV-Positive Large B-Cell Lymphoma With Features of Germinotropic Lymphoproliferative Disorder.

Int J Surg Pathol 2020 Oct 18;28(7):804-811. Epub 2020 May 18.

Hospital Clinico Universitario of Valencia, University of Valencia, Valencia, Spain.

Germinotropic lymphoproliferative disorder (GLPD) is a poorly characterized lymphoproliferative entity, recently included in the World Health Organization classification of hematolymphoid neoplasms. The histological pattern of this disease comprises monotypic plasmablasts that involve the germinal centers of the lymphoid follicles (germinotrophism), forming confluent aggregates positive for both human herpes virus type 8 (HHV8) and Epstein-Barr virus. Currently, after 17 years of its first description, only 18 cases have been reported. Read More

View Article and Full-Text PDF
October 2020

Isolation of Trypanosoma brucei brucei Infection-Derived Splenic Marginal Zone B Cells Based on CD1d/B220 Surface Expression in a Two-Step MACS-FACS Approach.

Methods Mol Biol 2020 ;2116:739-753

Biomedical Research Center (BMRC), Department of Environmental Tech, Food Tech, and Molecular Biotechnology, Ghent University, Incheon, South Korea.

Magnetic- and fluorescent-activated cell sorting (MACS and FACS) are used for isolation of distinct cell populations for subsequent studies including transcriptomics. The latter allows for the analysis of infection-induced alterations in gene expression profiles. MACS and FACS both use antibodies against cell surface molecules to isolate populations of interest. Read More

View Article and Full-Text PDF
February 2021

The prognostic significance of immune microenvironment in breast ductal carcinoma in situ.

Br J Cancer 2020 05 17;122(10):1496-1506. Epub 2020 Mar 17.

Nottingham Breast Cancer Research Centre, Division of Cancer and Stem Cells, School of Medicine, The University of Nottingham, Nottingham City Hospital, Nottingham, UK.

Background: The role of different subtypes of tumour infiltrating lymphocytes (TILs) in breast ductal carcinoma in situ (DCIS) is still poorly defined. This study aimed to assess the prognostic significance of B and T lymphocytes and immune checkpoint proteins expression in DCIS.

Methods: A well characterised DCIS cohort (n = 700) with long-term follow-up comprising pure DCIS (n = 508) and DCIS mixed with invasive carcinoma (IBC; n = 192) were stained immunohistochemically for CD20, CD3, CD4, CD8, FOXP3, PD1 and PDL1. Read More

View Article and Full-Text PDF

Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from L. Leaves.

Antioxidants (Basel) 2020 Feb 20;9(2). Epub 2020 Feb 20.

Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India.

Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from leaves. Read More

View Article and Full-Text PDF
February 2020

The many (sur)faces of B cells.

Biomed J 2019 08 3;42(4):201-206. Epub 2019 Oct 3.

University of Copenhagen, BRIC Biotech Research & Innovation Centre, Anders Lund Group, Ole Maaløes Vej 5, 2200 Copenhagen Denmark. Electronic address:

This issue of the Biomedical Journal is dedicated to the latest findings concerning the complex development and functions of B lymphocytes, including their origins during embryogenesis, their meticulous control by the CD22 receptor and different types of T cells, as well as the immunosuppressive abilities of certain B cell subsets. Furthermore, we learn about the complicated genetic background of a rare cardiac disease, the surgical outcomes of pure conus medullaris syndrome and occurrences of tuberculous spondylitis after percutaneous vertebroplasty. Finally, we discover that brain waves could very well be used for biometric authentication and that diffusion imaging displays good reproducibility through a spectrum of spatial resolutions. Read More

View Article and Full-Text PDF

Hashimoto Thyroiditis in Primary Thyroid Non-Hodgkin Lymphoma.

Am J Clin Pathol 2020 01;153(2):156-164

Department of Advanced Biomedical Sciences, Pathology Section, University of Naples "Federico II", Naples, Italy.

Objectives: To assess the prevalence of Hashimoto thyroiditis (HT) in primary thyroid lymphoma (PTL) and whether it differs between mucosa-associated lymphoid tissue (MALT) lymphoma and diffuse large B-cell lymphoma (DLBCL).

Methods: Electronic databases were searched for studies assessing HT prevalence in PTL, based on antithyroid antibodies, clinical history, or pathology. Pooled prevalence of HT and its association with histotype (MALT or DLBCL) were calculated. Read More

View Article and Full-Text PDF
January 2020

Traceless aptamer-mediated isolation of CD8 T cells for chimeric antigen receptor T-cell therapy.

Nat Biomed Eng 2019 10 17;3(10):783-795. Epub 2019 Jun 17.

Department of Bioengineering, University of Washington, Seattle, WA, USA.

Chimeric antigen receptor T-cell therapies using defined product compositions require high-purity T-cell isolation systems that, unlike immunomagnetic positive enrichment, are inexpensive and leave no trace on the final cell product. Here, we show that DNA aptamers (generated with a modified cell-SELEX procedure to display low-nanomolar affinity for the T-cell marker CD8) enable the traceless isolation of pure CD8 T cells at low cost and high yield. Captured CD8 T cells are released label-free by complementary oligonucleotides that undergo toehold-mediated strand displacement with the aptamer. Read More

View Article and Full-Text PDF
October 2019

Establishment and Expression of Cytokines in a -Infected Bovine B Cell Line.

Genes (Basel) 2019 04 30;10(5). Epub 2019 Apr 30.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou 730046, Gansu, China.

This study aimed to establish a pure single-cell -infected B cell line for the assessment of cytokine production in transformed and lipopolysaccharide (LPS)-stimulated cells. Several studies have aimed to identify cell surface markers in -transformed cells; however, no information on cytokine production in these cells is available. To investigate the potential of the transformed cells to produce cytokines and their potential responses to antigen-stimulation, we purified mature B cells (CD21) from the whole blood of cattle experimentally infected with the Kashi strain by magnetic separation. Read More

View Article and Full-Text PDF

Cutaneous localization of plasmablastic multiple myeloma with heterotopic expression of CD3 and CD4: Skin involvement revealing systemic disease.

J Cutan Pathol 2019 Aug 10;46(8):619-622. Epub 2019 May 10.

Department of Advanced Biomedical Sciences, Pathology Section, University of Naples Federico II, Naples, Italy.

Plasmablastic multiple myeloma is an uncommon morphological variant of multiple myeloma with aggressive clinical course and poor outcome. Its differential diagnosis includes plasmablastic lymphoma, a variant of diffuse large B-cell lymphoma with frequent extranodal presentation, which usually affects immunosuppressed patients and is virtually indistinguishable from plasmablastic multiple myeloma on the basis of histology solely. Differential diagnosis relies on close clinical-pathological correlation. Read More

View Article and Full-Text PDF

Clinical spectrum, evolution, and management of autoimmune cytopenias associated with angioimmunoblastic T-cell lymphoma.

Eur J Haematol 2019 Jul 30;103(1):35-42. Epub 2019 May 30.

Service de médecine interne, Hôpital Henri-Mondor, Assistance Publique-Hôpitaux de Paris (AP-HP), Université Paris-Est, Créteil, France.

Objective: Angioimmunoblastic T-cell lymphoma (AITL) is frequently associated with autoimmune cytopenia (AIC). Whether such patients have a particular phenotype and require particular management is unclear.

Method: Angioimmunoblastic T-cell lymphoma patients from the multicentric database of the Lymphoma Study Association presenting with AIC during disease course were included and matched to AITL patients without AIC (1/5 ratio). Read More

View Article and Full-Text PDF

The prevalence and prognostic significance of autoimmune cytopenias in a cohort of Egyptian patients with chronic lymphocytic leukemia.

Hematol Oncol Stem Cell Ther 2019 Jun 20;12(2):97-104. Epub 2019 Feb 20.

The Hematology Unit, Oncology Center, Mansoura University, Mansoura, Egypt. Electronic address:

Objective/background: The impact of autoimmune cytopenias (AICs) on the chronic lymphocytic leukemia (CLL) clinical course and its prognostic significance remain a matter of controversial debate. This could be due to exclusion of patients with cytopenia from most clinical trials for this particular complication and the lack of standard diagnostic criteria and treatment approaches. We herein evaluate the prevalence and the prognostic significance of AICs among patients with CLL. Read More

View Article and Full-Text PDF

Randall-type monoclonal immunoglobulin deposition disease: novel insights from a nationwide cohort study.

Blood 2019 02 21;133(6):576-587. Epub 2018 Dec 21.

Department of Nephrology, CHU Poitiers, Poitiers, France.

Monoclonal immunoglobulin deposition disease (MIDD) is a rare complication of B-cell clonal disorders, defined by Congo red negative-deposits of monoclonal light chain (LCDD), heavy chain (HCDD), or both (LHCDD). MIDD is a systemic disorder with prominent renal involvement, but little attention has been paid to the description of extrarenal manifestations. Moreover, mechanisms of pathogenic immunoglobulin deposition and factors associated with renal and patient survival are ill defined. Read More

View Article and Full-Text PDF
February 2019

Bidirectional effects of IL-10 regulatory B cells in Ldlr mice.

Atherosclerosis 2019 01 9;280:118-125. Epub 2018 Nov 9.

Division of BioTherapeutics, LACDR, Leiden University, Leiden, the Netherlands.

Background And Aims: Limiting the pro-inflammatory immune response is critical for the treatment of atherosclerosis. Regulatory B cells (Bregs) can modulate the immune response through interleukin-10 (IL-10). Current data regarding Bregs and atherosclerosis is scarce and conflicting. Read More

View Article and Full-Text PDF
January 2019

B cell responses to apoptotic cells in MFG-E8-/- mice.

Authors:
YuFeng Peng

PLoS One 2018 4;13(10):e0205172. Epub 2018 Oct 4.

Division of Rheumatology, Department of Medicine, University of Washington, Seattle, Washington, United States of America.

Defective clearance of apoptotic cells in MFG-E8 deficient mice results in lupus-like disease in the mixed B6x129, but not pure B6 background. The lack of overt autoimmunity in MFG-E8-/- B6 mice suggests that accumulation of apoptotic cells is not sufficient to break central tolerance. However, the delayed clearance of apoptotic cells in the follicles of MFG-E8-/- B6 mice provides an excellent opportunity to investigate how B cells respond to excessive apoptotic cells in the periphery under relatively non-inflammatory conditions. Read More

View Article and Full-Text PDF

Clinical and laboratory features of seventy-eight UK patients with Good's syndrome (thymoma and hypogammaglobulinaemia).

Clin Exp Immunol 2019 01 21;195(1):132-138. Epub 2018 Oct 21.

Immunology, University of Manchester, Manchester University Hospitals NHS Trust, Manchester, UK.

Good's syndrome (thymoma and hypogammaglobulinaemia) is a rare secondary immunodeficiency disease, previously reported in the published literature as mainly individual cases or small case series. We use the national UK-Primary Immune Deficiency (UKPID) registry to identify a large cohort of patients in the UK with this PID to review its clinical course, natural history and prognosis. Clinical information, laboratory data, treatment and outcome were collated and analysed. Read More

View Article and Full-Text PDF
January 2019

Autoimmune cytopenias in patients with chronic lymphocytic leukaemia treated with ibrutinib in routine clinical practice at an academic medical centre.

Br J Haematol 2018 11 16;183(3):421-427. Epub 2018 Aug 16.

Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA.

The effects of ibrutinib on the natural history of autoimmune cytopenias (AIC) among chronic lymphocytic leukaemia (CLL) patients treated in routine clinical practice require further investigation. Using the Mayo Clinical CLL Database, 193 CLL patients treated with ibrutinib between November 2013 and January 2017 outside the context of a clinical trial were identified; complete review of their medical records was performed for details of past history of AIC and treatment-emergent AIC. We identified 29/193 (15%) patients with history of AIC prior to ibrutinib start. Read More

View Article and Full-Text PDF
November 2018

Autoimmune cytopenias in chronic lymphocytic leukemia: a concise review and treatment recommendations.

Expert Rev Hematol 2018 08 14;11(8):613-624. Epub 2018 Jul 14.

a Department of Hematology and Lymphoma and Myeloma Center (LYMMCARE) , Academic Medical Center, University of Amsterdam , Amsterdam , The Netherlands.

Introduction: Chronic lymphocytic leukemia (CLL) is frequently complicated by cytopenias, either due to bone marrow infiltration or autoimmunity, resulting in autoimmune hemolytic anemia (AIHA), immune thrombocytopenia (ITP), pure red cell aplasia (PRCA), or autoimmune neutropenia (AIN). Morbidity due to autoimmune cytopenias (AIC) can be substantial; in addition, infection risk increases and pre-existing infections might deteriorate due to immunosuppressive medication. In the aging population, CLL occurs more frequently and AIC related to CLL represent a growing clinical challenge. Read More

View Article and Full-Text PDF

The devil is in the details: retention of recipient group A type 5 years after a successful allogeneic bone marrow transplant from a group O donor.

Immunohematology 2018 Jan;33(1):19-22

Michigan Medicine University of Michigan Ann Arbor, MI.

Conclusions: ABO-incompatible (ABOi) hematopoietic stem cell transplants (HSCTs) can present challenges in the blood bank. During transplantation, patients receive components that are ABO-compatible with both the donor graft and recipient; this practice can strain group O red blood cell (RBC) inventories.1 In addition, there are risks for acute hemolysis at the time of infusion and in the early post-transplant period. Read More

View Article and Full-Text PDF
January 2018

Development of an unbiased, semi-automated approach for classifying plasma cell immunophenotype following multicolor flow cytometry of bone marrow aspirates.

Cytometry B Clin Cytom 2018 09 6;94(5):602-610. Epub 2018 Apr 6.

Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

Background: Despite increased usage of multiparameter flow cytometry (MFC) to assess diagnosis, prognosis, and therapeutic efficacy (minimal residual disease, MRD) in plasma cell neoplasms (PCNs), standardization of methodology and data analysis is suboptimal. We investigated the utility of using the mean and median fluorescence intensities (FI) obtained from MFC to objectively describe parameters that distinguish plasma cell (PC) phenotypes.

Methods: In this retrospective study, flow cytometry results from bone marrow aspirate specimens from 570 patients referred to the Myeloma Institute at UAMS were evaluated. Read More

View Article and Full-Text PDF
September 2018

Myeloid cell leukemia-1 protein expression and myeloid cell leukemia-1 gene amplification in non small cell lung cancer.

Indian J Pathol Microbiol 2018 Jan-Mar;61(1):27-30

Department of Pathology, Albany Medical Center, Albany, New York, USA.

Background: Myeloid cell leukemia-1 (Mcl-1) is a member of the B-cell lymphoma 2 family known to play a significant role in the regulation of apoptosis. Mcl-1 expression has been studied in nonsmall cell lung cancer (NSCLC) cell lines but has not been previously evaluated as a prognostic factor in clinical samples.

Materials And Methods: Formalin-fixed, paraffin-embedded sections from 119 NSCLC, including 33 squamous cell carcinomas (SCC), 55 adenocarcinomas (AC), and 31 either pure adenocarcinoma in situ (AIS) or AC with lepidic features were immunostained by an automated method with rabbit polyclonal Mcl-1. Read More

View Article and Full-Text PDF
November 2018

Good's Syndrome-Association of the Late Onset Combined Immunodeficiency with Thymoma: Review of Literature and Case Report.

Iran J Allergy Asthma Immunol 2018 Feb;17(1):85-93

Pediatric Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Good's syndrome, the adult onset hypogammaglobulinemia associated with thymoma has been explained about six decades ago. It generally presents with recurrent infections and several paraneoplastic syndromes including myasthenia gravis, pure red cell aplasia, connective tissue disorders, superior vena cava, Horner's syndrome, lichen planus and inflammatory bowel disease. Lack of B cell, dysfunction of T cell, CD4+ T cell lymphopenia, reversed CD4/CD8+ T cell ratio, autoantibodies against Th17 related cytokines have been respected as the pathogenesis of the immune dysregulation this syndrome. Read More

View Article and Full-Text PDF
February 2018

Rational management approach to pure red cell aplasia.

Haematologica 2018 02 7;103(2):221-230. Epub 2017 Dec 7.

Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic, OH, USA

Pure red cell aplasia is an orphan disease, and as such lacks rationally established standard therapies. Most cases are idiopathic; a subset is antibody-mediated. There is overlap between idiopathic cases and those with T-cell large granular lymphocytic leukemia, hypogammaglobulinemia, and low-grade lymphomas. Read More

View Article and Full-Text PDF
February 2018

Proven Invasive Pulmonary Aspergillosis in Stem Cell Transplant Recipient Due to Aspergillus sublatus, a Cryptic Species of A. nidulans.

Mycopathologia 2018 Apr 11;183(2):423-429. Epub 2017 Nov 11.

Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, V Uvalu 84, 15006, Prague 5, Czech Republic.

Invasive fungal disease represents one of the severe complications in haematopoietic stem cell transplant recipients. We describe a case of a patient treated for relapse of chronic lymphoblastic leukaemia 6 years after HSCT. The patient was treated for invasive pulmonary aspergillosis but died 3 months later from multiple organ failures consisting of haemorrhagic necrotizing fungal pneumonia, refractory chronic hepatic graft versus host disease and cytomegalovirus hepatitis. Read More

View Article and Full-Text PDF