MUSE (Microscopy with UV Surface Excitation): a novel approach to real-time inexpensive slide free dermatopathology.
- Amir Qorbani,
- Farzad Fereidouni,
- Richard Levenson,
- Sana Yasmine Lahoubi,
- Zachary T Harmany,
- Austin Todd,
- Maxwell A Fung
J Cutan Pathol 2018 Apr 16. Epub 2018 Apr 16.
Department of Pathology and Laboratory Medicine, University of California, Sacramento, CA, USA.
Traditional histology relies on processing and physically sectioning either frozen or formalin-fixed, paraffin-embedded (FFPE) tissue into thin slices (typically 4-6 microns) prior to staining and viewing on a standard wide-field microscope. MUSE represents a novel alternative microscopy method that works with ultraviolet (UV) excitation using oblique cis-illumination which can generate high-quality images from the cut surface of fresh or fixed tissue after brief staining, with no requirement for fixation, embedding and histological sectioning of tissue specimens. We examined its potential utility in dermatopathology. Read More