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J Mol Biol 2022 Jun 3:167667. Epub 2022 Jun 3.

Microbial Biology, Ruhr University Bochum, Germany. Electronic address:

The type III secretion system (T3SS) is indispensable for successful host cell infection by many Gram-negative pathogens. The molecular syringe delivers effector proteins that suppress the host immune response. Synthesis of T3SS components in Yersinia pseudotuberculosis relies on host body temperature, which induces the RNA thermometer (RNAT)-controlled translation of lcrF coding for a virulence master regulator that activates transcription of the T3SS regulon. Read More

Biochim Biophys Acta Biomembr 2022 Sep 25;1864(9):183971. Epub 2022 May 25.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100-let Vladivostoku 159, 690022 Vladivostok, Russia.

The recombinant OmpF porin of Yersinia pseudotuberculosis as a model of transmembrane protein of the β-barrel structural family was used to study low growth temperature effect on the structure of the produced inclusion bodies (IBs). This porin showed a very low expression level in E. coli at a growth temperature below optimal 37 °C. Read More

PLoS Pathog 2022 May 25;18(5):e1010586. Epub 2022 May 25.

[This corrects the article DOI: 10.1371/journal.ppat. Read More

PLoS Pathog 2022 05 23;18(5):e1010251. Epub 2022 May 23.

Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany.

Yersinia enterocolitica employs a type three secretion system (T3SS) to translocate immunosuppressive effector proteins into host cells. To this end, the T3SS assembles a translocon/pore complex composed of the translocator proteins YopB and YopD in host cell membranes serving as an entry port for the effectors. The translocon is formed in a Yersinia-containing pre-phagosomal compartment that is connected to the extracellular space. Read More

Proc Natl Acad Sci U S A 2022 05 17;119(21):e2204044119. Epub 2022 May 17.

Univ. Lille, Inserm, CNRS, CHU Lille, Institut Pasteur Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, F-59000 Lille, France.

PLoS Pathog 2022 05 16;18(5):e1010556. Epub 2022 May 16.

W. Harry Feinstone Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.

Antibiotic tolerance is typically associated with a phenotypic change within a bacterial population, resulting in a transient decrease in antibiotic susceptibility that can contribute to treatment failure and recurrent infections. Although tolerant cells may emerge prior to treatment, the stress of prolonged antibiotic exposure can also promote tolerance. Here, we sought to determine how Yersinia pseudotuberculosis responds to doxycycline exposure, to then verify if these gene expression changes could promote doxycycline tolerance in culture and in our mouse model of infection. Read More

Bull Exp Biol Med 2022 Apr 3;172(6):725-728. Epub 2022 May 3.

G. P. Somov Research Institute of Epidemiology and Microbiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Vladivostok, Russia.

One of the mechanisms underlying the appearance of chronic infections is transition of pathogens into a non-culturable state, which is largely associated with the use of antibiotics. We studied ultrastructure of dormant bacteria Yersinia pseudotuberculosis obtained from the vegetative form of strain 512 by inhibition with kanamycin. On the model of the causative agent of pseudotuberculosis we showed that transition of prokaryotes to a dormant state occurs through apoptosis of bacteria. Read More

Epidemiol Mikrobiol Imunol 2022 ;71(1):32-39

Objective: The aim was to provide an overview of the basic epidemiological characteristics of human yersiniosis and to analyze the reported epidemiological data on the incidence of this disease in the Czech Republic (CZ) in 2018-2020.

Methods: A descriptive analysis was performed of cases of yersiniosis captured in the Infectious Disease Information System (ISIN) in the CZ in 2018-2020. MS Excel 2010 was used for data processing and sorting. Read More

Comp Immunol Microbiol Infect Dis 2022 Jul 8;86:101811. Epub 2022 Apr 8.

Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), Departamento de Sanidad Animal, Universidad de Córdoba, Córdoba, Spain; CIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Spain.

On January 2020, an outbreak of acute mortality was detected in the capybara (Hydrochoerus hydrochaeris) population kept in the Cordoba Zoo Conservation Center (CZCC) in Spain. Morbidity and mortality rates were 71%, and the fatality rate was 100%. Macroscopic examination revealed multifocal nodular lesions with fibrinonecrotic exudate in lungs and mesenteric lymph nodes. Read More

Cureus 2022 Mar 15;14(3):e23192. Epub 2022 Mar 15.

Community Medicine Management, Shimane University Faculty of Medicine, Izumo, JPN.

is a rare pathogen that causes yersiniosis, a foodborne disease that has become more prevalent in recent years Yersiniosis commonly causes gastrointestinal symptoms; however, bacteremia can be the primary clinical finding. Here, we report the case of an 83-year-old man who presented with fever and fatigue and was diagnosed with bacteremia. Gastrointestinal findings were absent at the time of admission. Read More

Microbiol Spectr 2022 Apr 19:e0224221. Epub 2022 Apr 19.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

The life cycle of Yersinia pestis has changed a lot to adapt to flea-borne transmission since it evolved from an enteric pathogen, Yersinia pseudotuberculosis. Small insertions and deletions (indels), especially frameshift mutations, can have major effects on phenotypes and contribute to virulence and host adaptation through gene disruption and inactivation. Here, we analyzed 365 Y. Read More

Vet Microbiol 2022 Jun 4;269:109424. Epub 2022 Apr 4.

Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöbergin katu 2, 00790 Helsinki, Finland.

A Yersinia pseudotuberculosis outbreak was diagnosed in a male turkey flock in Finland. Y. pseudotuberculosis is a quite rare zoonotic bacterium, which typically causes enteritis in humans and sudden death in animals. Read More

Proc Natl Acad Sci U S A 2022 04 11;119(17):e2116722119. Epub 2022 Apr 11.

Institute for Pre- and Protohistoric Archaeology and Archaeology of the Roman Provinces, Ludwig Maximilian University Munich, 80539 Munich, Germany.

The bacterial pathogen Yersinia pestis gave rise to devastating outbreaks throughout human history, and ancient DNA evidence has shown it afflicted human populations as far back as the Neolithic. Y. pestis genomes recovered from the Eurasian Late Neolithic/Early Bronze Age (LNBA) period have uncovered key evolutionary steps that led to its emergence from a Yersinia pseudotuberculosis-like progenitor; however, the number of reconstructed LNBA genomes are too few to explore its diversity during this critical period of development. Read More

Pediatr Int 2022 01;64(1):e15036

Department of Pediatrics, Oita University Faculty of Medicine, Yufu, Japan.

NPJ Biofilms Microbiomes 2022 03 29;8(1):13. Epub 2022 Mar 29.

Department of Molecular Biology, Umeå University, Umeå, Sweden.

Bacteria often reside in sessile communities called biofilms, where they adhere to a variety of surfaces and exist as aggregates in a viscous polymeric matrix. Biofilms are resistant to antimicrobial treatments, and are a major contributor to the persistence and chronicity of many bacterial infections. Herein, we determined that the CpxA-CpxR two-component system influenced the ability of enteropathogenic Yersinia pseudotuberculosis to develop biofilms. Read More

Acta Trop 2022 Jul 24;231:106427. Epub 2022 Mar 24.

Departamento de Microbiologia, Instituto Aggeu Magalhães; FIOCRUZ, Recife, Brazil. Electronic address:

Plague is a flea-borne zoonosis that affects a wide range of mammals and still causes outbreaks in human populations yearly across several countries. While crucial for proper treatment, early diagnosis is still a major challenge in low- and middle-income countries due to poor access to laboratory infrastructure in rural areas. To tackle this issue, we developed and evaluated a new Fraction 1 capsular antigen (F1)-based rapid diagnostic test (RDT) as an alternative method for plague serological diagnosis and surveillance in humans and other mammals. Read More

Proc Natl Acad Sci U S A 2022 03 11;119(11):e2109667119. Epub 2022 Mar 11.

Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208.

Significance, the etiologic agent of plague, has been responsible for high mortality in several epidemics throughout human history. This plague bacillus has been used as a biological weapon during human history and is currently one of the deadliest biological threats. Currently, no licensed plague vaccines are available in the Western world. Read More

Eur Biophys J 2022 Apr 9;51(3):257-264. Epub 2022 Mar 9.

Institute of Physiology of Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Komi Republic, 167982, Russian Federation.

The interactions of a microbial cell with host cells and humoral factors play an important role in the development of infectious diseases. The study of these mechanisms contributes to the development of effective methods for the treatment of bacterial infections. One of the possible approaches to studying bacterial adhesion to host cells is based on the use of the optical trap method. Read More

Klin Mikrobiol Infekc Lek 2021 Dec;27(4):158-162

Regional Hospital Pribram, Dept. clinical microbiology and parasitology, Pribram, Czech Republic, e-mail:

The article describes a case of a rare infection caused by Yersinia pseudotuberculosis in a five-year-old boy admitted to the hospital. The infection was manifested by the so-called right lower quadrant syndrome, or terminal ileitis. The Y. Read More

Pathogens 2022 Feb 21;11(2). Epub 2022 Feb 21.

Department of Wine, Food and Molecular Biosciences, Lincoln University, P.O. Box 85054, Lincoln 7647, New Zealand.

and are important causes of enteric illness worldwide. Rapid response to suspected foodborne outbreaks is hampered by the widespread use of cold enrichment methods that require incubation periods of 10-21 days. Although these species grow faster at elevated temperatures, part of the rationale for cold enrichment is that a key pathogenicity marker (pYV virulence plasmid) is said to be lost at elevated temperatures. Read More

Front Microbiol 2021 1;12:738818. Epub 2022 Feb 1.

Section for Genetics and Evolutionary Biology, Department of Biosciences, University of Oslo, Oslo, Norway.

adhesin A (YadA) is a key virulence factor of and . YadA is a trimeric autotransporter adhesin, a class of adhesins that have been shown to enable many Gram-negative pathogens to adhere to/interact with the host extracellular matrix proteins such as collagen, vitronectin, and fibronectin. Here, we show for the first time that YadA of serotype O:9 not only interacts with proteinaceous surface molecules but can also attach directly to glycan moieties. Read More

Eur J Med Chem 2022 Mar 24;231:114148. Epub 2022 Jan 24.

Department of Drug Design and Optimization (DDOP), Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Campus E8.1, 66123, Saarbrücken, Germany; Department of Pharmacy, Saarland University, Campus E8.1, 66123, Saarbrücken, Germany. Electronic address:

Small macrocyclic peptides are promising candidates for new anti-infective drugs. To date, such peptides have been poorly studied in the context of anti-virulence targets. Using phage display and a self-designed peptide library, we identified a cyclic heptapeptide that can bind the carbon storage regulator A (CsrA) from Yersinia pseudotuberculosis and displace bound RNA. Read More

Science 2021 06;372(6549)

The Joint Center for Infection and Immunity between Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center (Guangzhou, 510623, China) and Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China.

Host cells initiate cell death programs to limit pathogen infection. Inhibition of transforming growth factor-β-activated kinase 1 (TAK1) by pathogenic in macrophages triggers receptor-interacting serine/threonine-protein kinase 1 (RIPK1)-dependent caspase-8 cleavage of gasdermin D (GSDMD) and inflammatory cell death (pyroptosis). A genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screen to uncover mediators of caspase-8-dependent pyroptosis identified an unexpected role of the lysosomal FLCN-FNIP2-Rag-Ragulator supercomplex, which regulates metabolic signalling and the mechanistic target of rapamycin complex 1 (mTORC1). Read More

Front Microbiol 2021 3;12:634289. Epub 2022 Jan 3.

Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

is an important animal pathogen, particularly for birds, rodents, and monkeys, which is also able to infect humans. Indeed, an increasing number of reports have been published on zoo animals that were killed by this species. One option to treat diseased animals is the application of strictly lytic (virulent) phages. Read More

Mol Microbiol 2022 04 8;117(4):886-906. Epub 2022 Feb 8.

Department of Molecular Biology, Umeå University, Umeå, Sweden.

YscX was discovered as an essential part of the Yersinia type III secretion system about 20 years ago. It is required for substrate secretion and is exported itself. Despite this central role, its precise function and mode of action remain unknown. Read More

J Vet Diagn Invest 2022 Mar 17;34(2):334-338. Epub 2022 Jan 17.

Departments of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

A concurrent outbreak of infection with occurred in adult captive African lions (). Two 17-y-old male lions and one 14-y-old female lion developed respiratory distress, lethargy, ataxia, and hyporexia. Within 3-5 d of the onset of clinical signs, one male and the female lion died and were submitted for postmortem examination. Read More

AMB Express 2022 Jan 6;12(1). Epub 2022 Jan 6.

Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl St, 53114, Wroclaw, Poland.

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Read More

Ugeskr Laeger 2022 01;184(1)

Yersinia pseudotuberculosis is a Gram-negative bacterium causing infection in humans through contaminated water and/or food. The infection commonly occurs as gastroenteritis and fever, abdominal pain due to mesenteric lymphadenitis and diarrhoea. Bacteraemia is rare and is typically seen in immunocompromised patients and occurs with different clinical presentations like Far East scarlet-like fever, splenic abscess, or mimic appendicitis. Read More

Proc Natl Acad Sci U S A 2022 01;119(2)

Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY 12208;

Hemachromatosis (iron-overload) increases host susceptibility to siderophilic bacterial infections that cause serious complications, but the underlying mechanisms remain elusive. The present study demonstrates that oral infection with hyperyersiniabactin (Ybt) producing Δ mutant (termed Δ) results in severe systemic infection and acute mortality to hemochromatotic mice due to rapid disruption of the intestinal barrier. Transcriptome analysis of Δ-infected intestine revealed up-regulation in cytokine-cytokine receptor interactions, the complement and coagulation cascade, the NF-κB signaling pathway, and chemokine signaling pathways, and down-regulation in cell-adhesion molecules and Toll-like receptor signaling pathways. Read More

EcoSal Plus 2021 12 27;9(2):eESP00142021. Epub 2021 Oct 27.

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.

Yersinia pseudotuberculosis is an family member that is commonly transmitted by the fecal-oral route to cause infections. From the small intestine, Y. pseudotuberculosis can invade through Peyer's patches and lymph vessels to infect the mesenteric lymph nodes (MLNs). Read More