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    2864 results match your criteria Pseudotuberculosis Yersinia

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    RovM and CsrA Negatively Regulate Urease Expression in.
    Front Microbiol 2018 27;9:348. Epub 2018 Feb 27.
    State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, China.
    Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium. However, whether RovM regulates the urease activity inwas still unknown. Read More

    The invasin D protein fromselectively binds the Fab region of host antibodies and affects colonization of the intestine.
    J Biol Chem 2018 Mar 13. Epub 2018 Mar 13.
    Structural Biology, Helmholtz-Centre for Infection Research, Germany.
    is a Gram-negative bacterium and zoonotic pathogen responsible for a wide range of diseases, ranging from mild diarrhea, enterocolitis, lymphatic adenitis to persistent local inflammation. Theinvasin D (InvD) molecule belongs to the invasin (InvA)-type autotransporter proteins, but its structure and function remain unknown. In this study, we present the first crystal structure of InvD, analyzed its expression and function in a murine infection model, and identified its target molecule in the host. Read More

    Control ofHeme Uptake Genes inin Response to Iron Sources.
    Front Cell Infect Microbiol 2018 22;8:47. Epub 2018 Feb 22.
    Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA, United States.
    Despite the mammalian host actively sequestering iron to limit pathogenicity, heme (or hemin when oxidized) and hemoproteins serve as important sources of iron for many bloodborne pathogens. The HmuRSTUV hemin uptake system allowsspecies to uptake and utilize hemin and hemoproteins as iron sources. HmuR is a TonB-dependent outer membrane receptor for hemin and hemoproteins. Read More

    [Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical trap].
    Prikl Biokhim Mikrobiol 2017 Mar-Apr;53(2):234-43
    A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. Read More

    A single amino acid change in the response regulator PhoP acquired duringevolution affects PhoP target gene transcription and polymyxin B susceptibility.
    J Bacteriol 2018 Feb 12. Epub 2018 Feb 12.
    Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA
    , the causative agent of plague, evolved from the closely related pathogenDuring its emergence,is believed to have acquired its unique pathogenic characteristics through numerous gene gains/losses, genomic rearrangements and single nucleotide polymorphism (SNP) changes. One such SNP creates a single amino acid variation in the DNA binding domain of PhoP, the response regulator in the PhoP/PhoQ two-component system.and the basal human-avirulent strains ofharbor glycine at position 215 of PhoP, whereas the modern human-virulent strains (KIM and CO92) harbor serine at this residue. Read More

    Loss of CNFY toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency.
    PLoS Pathog 2018 Feb 1;14(2):e1006858. Epub 2018 Feb 1.
    Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    Gastrointestinal infections caused by enteric yersiniae can become persistent and complicated by relapsing enteritis and severe autoimmune disorders. To establish a persistent infection, the bacteria have to cope with hostile surroundings when they transmigrate through the intestinal epithelium and colonize underlying gut-associated lymphatic tissues. How the bacteria gain a foothold in the face of host immune responses is poorly understood. Read More

    Growth of Yersinia pseudotuberculosis Strains at Different Temperatures, pH Values, and NaCl and Ethanol Concentrations.
    J Food Prot 2018 Jan;81(1):142-149
    Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014 University of Helsinki, Helsinki, Finland (ORCID: [R.K.-T.]).
    Maximum growth temperature and growth limits in Luria-Bertani broth at different pH values and NaCl and ethanol concentrations were determined for 49 Yersinia pseudotuberculosis strains representing serotypes O:1, O:2, O:3, O:4, and O:5. In addition, the ability of the strains to grow at 0°C and the growth parameters at 1°C were determined. The maximum growth temperatures measured by Gradiplate temperature incubator varied between 42. Read More

    Effects of urbanization on host-pathogen interactions, using Yersinia in house sparrows as a model.
    PLoS One 2017 27;12(12):e0189509. Epub 2017 Dec 27.
    Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
    Urbanization strongly affects biodiversity, altering natural communities and often leading to a reduced species richness. Yet, despite its increasingly recognized importance, how urbanization impacts on the health of individual animals, wildlife populations and on disease ecology remains poorly understood. To test whether, and how, urbanization-driven ecosystem alterations influence pathogen dynamics and avian health, we use house sparrows (Passer domesticus) and Yersinia spp. Read More

    Crystal structure of d-glycero-α-d-manno-heptose-1-phosphate guanylyltransferase from Yersinia pseudotuberculosis.
    Biochim Biophys Acta 2018 Mar 23;1866(3):482-487. Epub 2017 Dec 23.
    College of Pharmacy, Ewha W. University, 52, Ewhayeodae-gil, Seoul, 03760, Republic of Korea. Electronic address:
    The Gram-negative bacterium Yersinia pseudotuberculosis is the causative agent of yersiniosis. d-glycero-α-d-manno-heptose-1-phosphate guanylyltransferase (HddC) is the fourth enzyme of the GDP-d-glycero-α-d-manno-heptose biosynthesis pathway which is important for the virulence of the microorganism. Therefore, HddC is a potential target of antibiotics against yersiniosis. Read More

    [Bile-resistant Gram-negative bacteria effect of different kinds of root decoction pieces].
    Zhongguo Zhong Yao Za Zhi 2017 Nov;42(21):4135-4141
    Medical School of Wuhan, University of Science and Technology, Wuhan 430065, China.
    To investigate the microbial contamination in Chinese herbal decoction pieces with different functional types by studying the total aerobic microbial count (TAMC), and total yeast and mould count (TYMC) in 40 samples of 8 types of root decoction pieces; further evaluate the contamination load of bile-resistant Gram-negative bacteria, and identify the Gram-negative bacteria by using biochemical identification system for Gram-negative bacteria. Our results showed that the TAMC value was more than 1 000 CFU•g⁻¹ in 85% (34/40) samples, and was more than 100 CFU•g⁻¹ in 30% (12/40) samples; the contamination of bile-resistant Gram-negative bacteria was detected in 45% (18/40) of the samples. The bile-resistant Gram-negative bacteria load of seven batches of samples was N>1 000 MPN•g⁻¹. Read More

    CCR2Inflammatory Monocytes Are Recruited to Yersinia pseudotuberculosis Pyogranulomas and Dictate Adaptive Responses at the Expense of Innate Immunity during Oral Infection.
    Infect Immun 2018 Mar 20;86(3). Epub 2018 Feb 20.
    Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA
    Murine Ly6Cinflammatory monocytes (IMs) require CCR2 to leave the bone marrow and enter mesenteric lymph nodes (MLNs) and other organs in response toinfection. We are investigating how IMs, which can differentiate into CD11cdendritic cells (DCs), contribute to innate and adaptive immunity toPreviously, we obtained evidence that IMs are important for a dominant CD8T cell response to the epitope YopEand host survival using intravenous infections with attenuatedHere we challenged CCR2or CCR2mice orally with wild-typeto investigate how IMs contribute to immune responses during intestinal infection. Unexpectedly, CCR2mice did not have reduced survival but retained body weight better and their MLNs clearedfaster and with reduced lymphadenopathy compared to controls. Read More

    Self-Organization of Recombinant Membrane Porin OmpF from Yersinia pseudotuberculosis in Aqueous Environments.
    Biochemistry (Mosc) 2017 Nov;82(11):1304-1313
    Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
    Recombinant porin OmpF (an integral protein of bacterial outer membrane) from Yersinia pseudotuberculosis was synthesized in Escherichia coli cells as inclusion bodies. By combining the methods of anion-exchange and gel filtration chromatographies, recombinant OmpF (rOmpF) was isolated as an individual protein in its denatured state, and its characteristic properties (molecular mass, N-terminal amino acid sequence, and hydrodynamic radius of the protein in 8 M urea solution) were determined. According to the data of gel filtration, dynamic light scattering, optical spectroscopy, and binding of the hydrophobic fluorescent probe 8-anilino-1-naphthalenesulfonic acid, the rOmpF is fully unfolded in 8 M urea and exists in random coil conformation. Read More

    White Adipose Tissue Is a Reservoir for Memory T Cells and Promotes Protective Memory Responses to Infection.
    Immunity 2017 Dec 5;47(6):1154-1168.e6. Epub 2017 Dec 5.
    Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA; NIAID Microbiome Program, NIH, Bethesda, MD 20892, USA. Electronic address:
    White adipose tissue bridges body organs and plays a fundamental role in host metabolism. To what extent adipose tissue also contributes to immune surveillance and long-term protective defense remains largely unknown. Here, we have shown that at steady state, white adipose tissue contained abundant memory lymphocyte populations. Read More

    Effects of host cell sterol composition upon internalization ofand clustered β1 integrin.
    J Biol Chem 2018 Jan 2;293(4):1466-1479. Epub 2017 Dec 2.
    From the Departments of Biochemistry and Cell Biology and
    is a foodborne pathogenic bacterium that causes acute gastrointestinal illness, but its mechanisms of infection are incompletely described. We examined how host cell sterol composition affecteduptake. To do this, we depleted or substituted cholesterol in human MDA-MB-231 epithelial cells with various alternative sterols. Read More

    Bioluminescent tracing of a Yersinia pestis pCD1-mutant and Yersinia pseudotuberculosis in subcutaneously infected mice.
    Microbes Infect 2018 Mar 26;20(3):166-175. Epub 2017 Nov 26.
    Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. Electronic address:
    Yersinia pestis has evolved from Yersinia pseudotuberculosis serotype O:1b. A typical Y. pestis contains three plasmids: pCD1, pMT1 and pPCP1. Read More

    Phylogeographic separation and formation of sexually discrete lineages in a global population of.
    Microb Genom 2017 Oct 18;3(10):e000133. Epub 2017 Sep 18.
    Institute of Microbiology and Infection, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK.
    is a Gram-negative intestinal pathogen of humans and has been responsible for several nationwide gastrointestinal outbreaks. Large-scale population genomic studies have been performed on the other human pathogenic species of the genusandallowing a high-resolution understanding of the ecology, evolution and dissemination of these pathogens. However, to date no purpose-designed large-scale global population genomic analysis ofhas been performed. Read More

    Investigation of the Physical and Bioactive Properties of Bromo- and Iodo-Containing Sponge-Derived Compounds Possessing an Oxyphenylethanamine Core.
    J Nat Prod 2017 Dec 16;80(12):3255-3266. Epub 2017 Nov 16.
    Department of Chemistry and Biochemistry, University of California , Santa Cruz, California 95064, United States.
    This research set out to identify compounds from marine sponges that can act as bacterial virulence blockers. Extracts from a total of 80 sponges collected from throughout Indonesia were screened in a high-throughput NF-κB-based screen that identifies compounds capable of inhibiting the bacterial type III secretion system (T3SS) in Yersinia pseudotuberculosis. An extract that was shown to inhibit T3SS-driven NF-κB expression was obtained from an Iotrochota cf. Read More

    ArfGAP1 restrictsentry by controlling the actin cytoskeleton.
    EMBO Rep 2018 Jan 15;19(1):29-42. Epub 2017 Nov 15.
    CNRS, Inserm, CHU Lille, U1019 - UMR8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Univ. Lille, Lille, France
    The interaction of(Mtb) with pulmonary epithelial cells is critical for early stages of bacillus colonization and during the progression of tuberculosis. Entry of Mtb into epithelial cells has been shown to depend on F-actin polymerization, though the molecular mechanisms are still unclear. Here, we demonstrate that mycobacterial uptake into epithelial cells requires rearrangements of the actin cytoskeleton, which are regulated by ADP-ribosylation factor 1 (Arf1) and phospholipase D1 (PLD1), and is dependent on the M3 muscarinic receptor (MR). Read More

    Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene.
    Braz J Microbiol 2018 Apr - Jun;49(2):320-328. Epub 2017 Oct 18.
    Tianjin University, School of Life Sciences, Tianjin, China. Electronic address:
    Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. Read More

    Multiple factors correlating with wing malformations in the population of Parnassius apollo (Lepidoptera: Papilionidae) restituted from a low number of individuals: A mini review.
    Insect Sci 2017 Nov 2. Epub 2017 Nov 2.
    Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
    The Apollo butterfly, Parnassius apollo (Linnaeus), was common in Europe over 100 years ago, but currently it is considered as near threatened. Different conservation programs have promoted the persistence of this species; however, it is still endangered. An example of such programs was the action devoted to reestablish the Apollo butterfly population in Pieniny National Park (Poland) from only 20-30 individuals which had survived till the last decade of the 20th century. Read More

    Far East Scarlet-like Fever Masquerading as Adult-onset Kawasaki Disease.
    Intern Med 2018 Feb 1;57(3):437-440. Epub 2017 Nov 1.
    Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Japan.
    A previously healthy 31-year-old man was referred to us with refractory septic shock accompanied by bilateral conjunctival congestion and erythema of his right lower limb. Nine days after admission, he had bilateral desquamation of the fingertips, and his presentation satisfied the criteria for Kawasaki disease. A serological examination was positive for Yersinia pseudotuberculosis, and he was diagnosed with Far East scarlet-like fever (FESLF). Read More

    In silico and in vitro analysis of cross-reactivity between Yersinia pseudotuberculosis OmpF porin and thyroid-stimulating hormone receptor.
    Int J Biol Macromol 2018 Feb 25;107(Pt B):2484-2491. Epub 2017 Oct 25.
    G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, Vladivostok, 690022, Russia.
    This work is devoted to the ascertainment of serological cross-reactivity between OmpF porin from Yersinia pseudotuberculosis (YpOmpF) and human thyroid-stimulating hormone receptor (hTSHR). Extracts containing hTSHR were isolated from surgical thyroid tissue of patients with clinical and diagnostic signs of diffuse toxic goiter. Monoclonal antibodies to hTSHR (mAbs) were shown to interact both with antigens in thyroid tissue extracts and with YpOmpF. Read More

    The Product of Yersinia pseudotuberculosis mcc Operon Is a Peptide-Cytidine Antibiotic Activated Inside Producing Cells by the TldD/E Protease.
    J Am Chem Soc 2017 11 31;139(45):16178-16187. Epub 2017 Oct 31.
    Institute of Gene Biology , Russian Academy of Science, 34/5 Vavilov str., 119334 Moscow, Russia.
    Microcin C is a heptapeptide-adenylate antibiotic produced by some strains of Escherichia coli. Its peptide part is responsible for facilitated transport inside sensitive cells where it is proteolyzed with release of a toxic warhead-a nonhydrolyzable aspartamidyl-adenylate, which inhibits aspartyl-tRNA synthetase. Recently, a microcin C homologue from Bacillus amyloliquefaciens containing a longer peptide part modified with carboxymethyl-cytosine instead of adenosine was described, but no biological activity of this compound was revealed. Read More

    The impact of length variations in the L2 loop on the structure and thermal stability of non-specific porins: The case of OmpCs from the Yersinia pseudotuberculosis complex.
    Biochim Biophys Acta 2018 Feb 14;1860(2):515-525. Epub 2017 Oct 14.
    G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia. Electronic address:
    Porins are integral proteins of the outer membranes of gram-negative bacteria. In membranes, they exist as homotrimers and the L2 loops contribute to their stability. Comparison of OmpC porins of the Yersinia pseudotuberculosis complex with other enterobacterial porins demonstrated L2 loop length diversity, which is caused by varying numbers of dipeptide/tripeptide repeats. Read More

    Multiple Small Intestine Ulcers with Desquamation of the Fingers.
    Case Rep Gastroenterol 2017 May-Aug;11(2):452-461. Epub 2017 Aug 17.
    Department of Gastroenterology, Ishikawa Prefectural Central Hospital, Kanazawa, Japan.
    A 73-year-old female was admitted to our hospital with abdominal pain and diarrhea. Computed tomography detected distension of the small intestine. A palmar erythema, multiple oral ulcers, and desquamation of the fingers appeared after hospitalization. Read More

    Plasmid-mediated doxycycline resistance in a Yersinia pestis strain isolated from a rat.
    Int J Antimicrob Agents 2018 Feb 10;51(2):249-254. Epub 2017 Oct 10.
    Yersinia Research Unit, WHO Collaborating Center, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France. Electronic address:
    The emergence of antibiotic-resistant Yersinia pestis strains represents a public health concern. Two antibiotic-resistant Y. pestis strains isolated from Madagascar have been previously identified and characterised. Read More

    Differential impact of lipopolysaccharide defects caused by loss of RfaH in Yersinia pseudotuberculosis and Yersinia pestis.
    Sci Rep 2017 Sep 7;7(1):10915. Epub 2017 Sep 7.
    Department of Microbiology and Molecular Biology, 4007 LSB, Brigham Young University, Provo, UT, 84602, USA.
    RfaH enhances transcription of a select group of operons controlling bacterial surface features such as lipopolysaccharide (LPS). Previous studies have suggested that rfaH may be required for Yersinia pseudotuberculosis resistance to antimicrobial chemokines and survival during mouse infections. In order to further investigate the role of RfaH in LPS synthesis, resistance to host defense peptides, and virulence of Yersinia, we constructed ΔrfaH mutants of Y. Read More

    RIPK1-dependent apoptosis bypasses pathogen blockade of innate signaling to promote immune defense.
    J Exp Med 2017 Nov 30;214(11):3171-3182. Epub 2017 Aug 30.
    Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA
    Many pathogens deliver virulence factors or effectors into host cells in order to evade host defenses and establish infection. Although such effector proteins disrupt critical cellular signaling pathways, they also trigger specific antipathogen responses, a process termed "effector-triggered immunity." The Gram-negative bacterial pathogeninactivates critical proteins of the NF-κB and MAPK signaling cascade, thereby blocking inflammatory cytokine production but also inducing apoptosis. Read More

    Oral delivery of Escherichia coli persistently infected with M2e-displaying bacteriophages partially protects against influenza A virus.
    J Control Release 2017 Oct 24;264:55-65. Epub 2017 Aug 24.
    VIB-UGent Center for Medical Biotechnology, VIB, Technologiepark, 927, Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, Technologiepark 927, Ghent, Belgium. Electronic address:
    We describe a novel live oral vaccine type. Conceptually, this vaccine is based on a non-lytic, recombinant filamentous bacteriophage that displays an antigen of interest. To provide proof of concept we used the amino-terminal part of a conserved influenza A virus epitope, i. Read More

    C4B gene influences intestinal microbiota through complement activation in patients with paediatric-onset inflammatory bowel disease.
    Clin Exp Immunol 2017 Dec 25;190(3):394-405. Epub 2017 Sep 25.
    Immunobiology, Research Programs Unit, University of Helsinki, Helsinki, Finland.
    Complement C4 genes are linked to paediatric inflammatory bowel disease (PIBD), but the mechanisms have remained unclear. We examined the influence of C4B gene number on intestinal microbiota and in-vitro serum complement activation by intestinal microbes in PIBD patients. Complement C4A and C4B gene numbers were determined by genomic reverse transcription-polymerase chain reaction (RT-PCR) from 64 patients with PIBD (Crohn's disease or ulcerative colitis). Read More

    Molecular bases for multidrug resistance in Yersinia pseudotuberculosis.
    Int J Med Microbiol 2017 Oct 16;307(7):371-381. Epub 2017 Aug 16.
    Yersinia Research Unit and Yersinia National Reference Laboratory, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris cedex 15, France. Electronic address:
    The enteropathogen Yersinia pseudotuberculosis causes gastrointestinal infections in humans. Although this species is usually susceptible to antibiotics active against Gram-negative bacteria, we identified three multidrug resistant (MDR) strains of Y. pseudotuberculosis that were isolated from the environment in Russia and from a patient in France. Read More

    Guanylate Binding Proteins Regulate Inflammasome Activation in Response to Hyperinjected Yersinia Translocon Components.
    Infect Immun 2017 10 20;85(10). Epub 2017 Sep 20.
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Gram-negative bacterial pathogens utilize virulence-associated secretion systems to inject, or translocate, effector proteins into host cells to manipulate cellular processes and promote bacterial replication. However, translocated bacterial products are sensed byucleotide binding domain andeucine-richepeat-containing proteins (NLRs), which trigger the formation of a multiprotein complex called the inflammasome, leading to secretion of interleukin-1 (IL-1) family cytokines, pyroptosis, and control of pathogen replication. Pathogenicbacteria inject effector proteins termed Yops, as well as pore-forming proteins that comprise the translocon itself, into target cells. Read More

    Engineering of Chimeric Protein Based on E Protein Domain III of Tick- Borne Encephalitis Virus and OmpF Porin of Yersinia pseudotuberculosis.
    Protein Pept Lett 2017 ;24(10):974-981
    Far Eastern Federal University, Vladivostok. Russian Federation.
    Background: Tick-borne encephalitis poses a serious public health threat in the endemic regions. The disease treatment is restricted to symptomatic therapy, so great expectations are in the development of the prophylactic and therapeutic vaccines. The domain III of E protein of the tickborne encephalitis virus is the main antigenic domain which includes virus-specific epitopes recognized by neutralizing antibodies. Read More

    Development of a novel multiplexed qPCR and Pyrosequencing method for the detection of human pathogenic yersiniae.
    Int J Food Microbiol 2017 Sep 20;257:247-253. Epub 2017 Jun 20.
    Canadian Food Inspection Agency, Lethbridge Laboratory, Township Rd 9-1, Lethbridge, Alberta T1J 3Z4, Canada. Electronic address:
    The purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. Yersinia enterocolitica, Y. Read More

    Synthetic Cyclic Peptomers as Type III Secretion System Inhibitors.
    Antimicrob Agents Chemother 2017 Sep 24;61(9). Epub 2017 Aug 24.
    Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, California, USA
    Antibiotic-resistant bacteria are an emerging threat to global public health. New classes of antibiotics and tools for antimicrobial discovery are urgently needed. Type III secretion systems (T3SS), which are required by dozens of Gram-negative bacteria for virulence but largely absent from nonpathogenic bacteria, are promising virulence blocker targets. Read More

    Epigallocatechin gallate inhibits the type III secretion system of Gram-negative enteropathogenic bacteria under model conditions.
    FEMS Microbiol Lett 2017 Jul;364(13)
    Department of Bacteriology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
    Epigallocatechin gallate (EGCG), a major polyphenol in green tea, inhibits the type III secretion system (T3SS) of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), Salmonella enterica serovar Typhimurium, and Yersinia pseudotuberculosis. The inhibitory effect causes the inhibition of hemolysis, cell invasion, cell adhesion and apoptosis, which are functions of the type III secretion device. In the case of EPEC, EspB accumulates in the cells. Read More

    CLAFEM: Correlative light atomic force electron microscopy.
    Methods Cell Biol 2017 21;140:165-185. Epub 2017 Apr 21.
    Univ. Lille, CNRS UMR 8204, Inserm U1019, CHU Lille, Institut Pasteur de Lille - CIIL - Center for Infection and Immunity of Lille, Lille, France.
    Atomic force microscopy (AFM) is becoming increasingly used in the biology field. It can give highly accurate topography and biomechanical quantitative data, such as adhesion, elasticity, and viscosity, on living samples. Nowadays, correlative light electron microscopy is a must-have tool in the biology field that combines different microscopy techniques to spatially and temporally analyze the structure and function of a single sample. Read More

    Structure and gene cluster of a tyvelose-containing O-polysaccharide of an entomopathogenic bacterium Yersinia entomophaga MH96related to Yersinia pseudotuberculosis.
    Carbohydr Res 2017 Jun 15;445:93-97. Epub 2017 Apr 15.
    State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russian Federation. Electronic address:
    An O-polysaccharide was isolated from the lipopolysaccharide of an entomopathogenic bacterium Yersinia entomophaga MH96by mild acid hydrolysis and studied by 2D NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: where Tyv indicates 3,6-dideoxy-d-arabino-hexose (tyvelose). The structure established is consistent with the gene content of the O-antigen gene cluster. Read More

    Experimental infection with Yersinia pseudotuberculosis in European brown hare (Lepus europaeus, Pallas).
    Asian Pac J Trop Med 2017 Mar 6;10(3):285-291. Epub 2017 Mar 6.
    Dipartimento di Scienze Veterinarie, Università di Pisa, Pisa, Italy.
    Objective: To investigate clinicopathological, bacteriological and pathological aspects of an experimental infection with Yersinia pseudotuberculosis (Y. pseudotuberculosis) in hares to verify the efficacy of serology for the in vivo diagnosis. Moreover, the pathogenicity of two Y. Read More

    Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders.
    PLoS One 2017 12;12(4):e0175648. Epub 2017 Apr 12.
    Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
    Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. Read More

    Salicylidene Acylhydrazides and Hydroxyquinolines Act as Inhibitors of Type Three Secretion Systems in Pseudomonas aeruginosa by Distinct Mechanisms.
    Antimicrob Agents Chemother 2017 Jun 24;61(6). Epub 2017 May 24.
    Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium
    Type 3 secretion systems (T3SSs) are major virulence factors in Gram-negative bacteria.expresses two T3SSs, namely, an injectisome (iT3SS) translocating effector proteins in the host cell cytosol and a flagellum (fT3SS) ensuring bacterial motility. Inhibiting these systems is an appealing therapeutic strategy for acute infections. Read More

    HmuS from Yersinia pseudotuberculosis is a non-canonical heme-degrading enzyme to acquire iron from heme.
    Biochim Biophys Acta 2017 07 4;1861(7):1870-1878. Epub 2017 Apr 4.
    Department of Biological Chemistry, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. Electronic address:
    Some Gram-negative pathogens import host heme into the cytoplasm and utilize it as an iron source for their survival. We report here that HmuS, encoded by the heme utilizing system (hmu) locus, cleaves the protoporphyrin ring to release iron from heme. A liquid chromatography/mass spectrometry analysis revealed that the degradation products of this reaction are two biliverdin isomers that result from transformation of a verdoheme intermediate. Read More

    Local Inflammatory Cues Regulate Differentiation and Persistence of CD8Tissue-Resident Memory T Cells.
    Cell Rep 2017 Apr;19(1):114-124
    Department of Immunology, University of Washington, Seattle, WA 98109, USA. Electronic address:
    Many pathogens initiate infection at mucosal surfaces, and tissue-resident memory T (Trm) cells play an important role in protective immunity, yet the tissue-specific signals that regulate Trm differentiation are poorly defined. During Yersinia infection, CD8T cell recruitment to areas of inflammation within the intestine is required for differentiation of the CD103CD69Trm subset. Intestinal proinflammatory microenvironments have elevated interferon (IFN)-β and interleukin-12 (IL-12), which regulated Trm markers, including CD103. Read More

    Yersinia pseudotuberculosis supports Th17 differentiation and limits de novo regulatory T cell induction by directly interfering with T cell receptor signaling.
    Cell Mol Life Sci 2017 08 4;74(15):2839-2850. Epub 2017 Apr 4.
    Department Experimental Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124, Brunswick, Germany.
    Adaptive immunity critically contributes to control acute infection with enteropathogenic Yersinia pseudotuberculosis; however, the role of CD4T cell subsets in establishing infection and allowing pathogen persistence remains elusive. Here, we assessed the modulatory capacity of Y. pseudotuberculosis on CD4T cell differentiation. Read More

    A starvation-induced regulator, RovM, acts as a switch for planktonic/biofilm state transition in Yersinia pseudotuberculosis.
    Sci Rep 2017 04 4;7(1):639. Epub 2017 Apr 4.
    State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
    The transition between the planktonic state and the biofilm-associated state is a key developmental decision for pathogenic bacteria. Biofilm formation by Yersinia pestis is regulated by hmsHFRS genes (β-1, 6-N-acetyl-D-glucosamine synthesis operon) in its flea vector and in vitro. However, the mechanism of biofilm formation in Yersinia pseudotuberculosis remains elusive. Read More

    Structure of the Y. pseudotuberculosis adhesin InvasinE.
    Protein Sci 2017 Jun 16;26(6):1182-1195. Epub 2017 Apr 16.
    Structural Biology of Autophagy Group, Department of Structure and Function of Proteins, Helmholtz Centre for Infection Research, Braunschweig, 38124, Germany.
    Enteropathogenic Yersinia expresses several invasins that are fundamental virulence factors required for adherence and colonization of tissues in the host. Within the invasin-family of Yersinia adhesins, to date only Invasin has been extensively studied at both structural and functional levels. In this work, we structurally characterize the recently identified inverse autotransporter InvasinE from Yersinia pseudotuberculosis (formerly InvasinD from Yersinia pseudotuberculosis strain IP31758) that belongs to the invasin-family of proteins. Read More

    Genetics and evolution of Yersinia pseudotuberculosis O-specific polysaccharides: a novel pattern of O-antigen diversity.
    FEMS Microbiol Rev 2017 03;41(2):200-217
    School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.
    O-antigen polysaccharide is a major immunogenic feature of the lipopolysaccharide of Gram-negative bacteria, and most species produce a large variety of forms that differ substantially from one another. There are 18 known O-antigen forms in the Yersinia pseudotuberculosis complex, which are typical in being composed of multiple copies of a short oligosaccharide called an O unit. The O-antigen gene clusters are located between the hemH and gsk genes, and are atypical as 15 of them are closely related, each having one of five downstream gene modules for alternative main-chain synthesis, and one of seven upstream modules for alternative side-branch sugar synthesis. Read More

    Yersinia spp. in Wild Rodents and Shrews in Finland.
    Vector Borne Zoonotic Dis 2017 05 23;17(5):303-311. Epub 2017 Mar 23.
    1 Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki , Helsinki, Finland .
    Yersinia enterocolitica and Yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in Europe. All Y. pseudotuberculosis strains are considered pathogenic, while Y. Read More

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