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    2878 results match your criteria Pseudotuberculosis Yersinia

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    Wzx flippases exhibiting complex O-unit preferences require a new model for Wzx-substrate interactions.
    Microbiologyopen 2018 Jun 10:e00655. Epub 2018 Jun 10.
    School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales, Australia.
    The Wzx flippase is a critical component of the O-antigen biosynthesis pathway, being responsible for the translocation of oligosaccharide O units across the inner membrane in Gram-negative bacteria. Recent studies have shown that Wzx has a strong preference for its cognate O unit, but the types of O-unit structural variance that a given Wzx can accommodate are poorly understood. In this study, we identified two Yersinia pseudotuberculosis Wzx that can distinguish between different terminal dideoxyhexose sugars on a common O-unit main-chain, despite both being able to translocate several other structurally-divergent O units. Read More

    Grasping the nettle: A bacterial invasin that targets immunoglobulin variable domains.
    J Biol Chem 2018 Jun;293(22):8691-8692
    From the Schools of Chemistry and Biological Sciences, University of Edinburgh, Edinburgh EH9 3FJ, Scotland, United Kingdom.
    In a new paper, the protein InvD from , a zoonotic pathogen, is shown to assist late-stage invasion of intestinal epithelia. Remarkably, InvD acts by binding the Fab region of IgG or IgA. It straddles adjacent light-chain and heavy-chain variable domains, but its binding is different from that of antigens in that complementarity-determining regions do not participate. Read More

    High-throughput analysis of Yersinia pseudotuberculosis gene essentiality in optimised in vitro conditions, and implications for the speciation of Yersinia pestis.
    BMC Microbiol 2018 May 31;18(1):46. Epub 2018 May 31.
    Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.
    Background: Yersinia pseudotuberculosis is a zoonotic pathogen, causing mild gastrointestinal infection in humans. From this comparatively benign pathogenic species emerged the highly virulent plague bacillus, Yersinia pestis, which has experienced significant genetic divergence in a relatively short time span. Much of our knowledge of Yersinia spp. Read More

    The development of a screening protocol for Salmonella spp. and enteropathogenic Yersinia spp. in samples from wild boar (Sus scrofa) also generating MLVA-data for Y. enterocolitica and Y. pseudotuberculosis.
    J Microbiol Methods 2018 Jul 21;150:32-38. Epub 2018 May 21.
    Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden; Department of Microbiology, National Veterinary Institute, Uppsala, Sweden. Electronic address:
    Salmonellosis and yersiniosis are notifiable human diseases that are commonly associated with contaminated food. Domestic pigs as well as wild boars and other wild-life have been identified as reservoirs of these bacteria. Methods for cultivation and molecular epidemiological investigations of Salmonella spp. Read More

    Effects of Psidium guajava leaf extract on secretion systems of Gram-negative enteropathogenic bacteria.
    Microbiol Immunol 2018 May 23. Epub 2018 May 23.
    Department of Bacteriology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa 903- 0215, Japan.
    We screened a total of 672 plant-tissue extracts to search for phytochemicals that inhibit the function of the type III secretion system (T3SS) of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). Among candidates examined, we found that an extract from the leaves of Psidium guajava (guava) inhibited the secretion of the EspB protein from EPEC and EHEC without affecting bacterial growth. Read More

    YopN is required for efficient effector translocation and virulence in .
    Infect Immun 2018 May 14. Epub 2018 May 14.
    Department of Molecular Biology, Umeå Centre for Microbial Research UCMR, Umeå University, Umeå, Sweden
    Type III Secretion Systems (T3SSs) are used by various Gram-negative pathogens to subvert the host defense by a host cell contact-dependent mechanism to secrete and translocate virulence effectors. While the effectors differ between pathogens and determine the pathogenic life style, the overall mechanism of secretion and translocation is conserved. T3SSs are regulated at multiple levels and some secreted substrates have also been shown to function in regulation. Read More

    Complete Genome Sequences of 10 Yersinia pseudotuberculosis Isolates Recovered from Wild Boars in Germany.
    Genome Announc 2018 May 10;6(19). Epub 2018 May 10.
    German Federal Institute for Risk Assessment, Berlin, Germany
    We report here the draft genome sequences of 10 isolates recovered from tonsils of wild boars hunted between 2015 and 2016 in Germany. Whole-genome sequencing and bioinformatic analyses were performed to assess the diversity of , which may result in human infections caused by the consumption of game meat. Read More

    Outbreaks of diarrhoea ('winter scours') in weaned Merino sheep in south-eastern Australia.
    Aust Vet J 2018 May;96(5):176-183
    Mackinnon Project, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.
    Objective: Identify the cause of outbreaks of diarrhoea during winter that are not attributable to gastrointestinal nematodes in weaned Merino sheep in the high rainfall regions of south-eastern Australia and determine the efficacy of antimicrobials used to treat this syndrome.

    Methods: We investigated 45 outbreaks on 24 farms. Faecal samples from affected animals were cultured for Yersinia, Campylobacter and Salmonella spp. Read More

    A longitudinal study of faecal shedding of Yersinia enterocolitica and Yersinia pseudotuberculosis by Merino lambs in south-eastern Australia.
    Prev Vet Med 2018 May 1;153:30-41. Epub 2018 Mar 1.
    Mackinnon Project, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, 250 Princes Highway, Werribee, VIC 3030, Australia.
    A prospective longitudinal study was conducted to investigate potential risk factors for faecal shedding of Yersinia enterocolitica and Y. pseudotuberculosis by Merino lambs in four flocks in south-eastern Australia. The primary aims of the study were to determine the seasonal patterns of shedding of pathogenic Y. Read More

    A Transcription Factor-Based Biosensor for Detection of Itaconic Acid.
    ACS Synth Biol 2018 May 17;7(5):1436-1446. Epub 2018 Apr 17.
    BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences , The University of Nottingham , Nottingham , NG7 2RD , United Kingdom.
    Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of itaconate including Aspergillus terreus, Escherichia coli, and Saccharomyces cerevisiae. However, the number of strains and conditions that can be tested for increased itaconate titers are currently limited because of the lack of high-throughput screening methods. Read More

    Heterologous Complementation Studies With the YscX and YscY Protein Families Reveals a Specificity for Type III Secretion.
    Front Cell Infect Microbiol 2018 16;8:80. Epub 2018 Mar 16.
    Department of Molecular Biology, Umeå University, Umeå, Sweden.
    Type III secretion systems harbored by several Gram-negative bacteria are often used to deliver host-modulating effectors into infected eukaryotic cells. About 20 core proteins are needed for assembly of a secretion apparatus. Several of these proteins are genetically and functionally conserved in type III secretion systems of bacteria associated with invertebrate or vertebrate hosts. Read More

    Genome rearrangements and phylogeny reconstruction in .
    PeerJ 2018 27;6:e4545. Epub 2018 Mar 27.
    Kharkevich Institute for Information Transmission Problems, Moscow, Russia.
    Genome rearrangements have played an important role in the evolution of from its progenitor . Traditional phylogenetic trees for based on sequence comparison have short internal branches and low bootstrap supports as only a small number of nucleotide substitutions have occurred. On the other hand, even a small number of genome rearrangements may resolve topological ambiguities in a phylogenetic tree. Read More

    Pangenomic Definition of Prokaryotic Species and the Phylogenetic Structure of spp.
    Front Microbiol 2018 12;9:428. Epub 2018 Mar 12.
    A.A.Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences (RAS), Moscow, Russia.
    The pangenome is the collection of all groups of orthologous genes (OGGs) from a set of genomes. We apply the pangenome analysis to propose a definition of prokaryotic species based on identification of lineage-specific gene sets. While being similar to the classical biological definition based on allele flow, it does not rely on DNA similarity levels and does not require analysis of homologous recombination. Read More

    RovM and CsrA Negatively Regulate Urease Expression in .
    Front Microbiol 2018 27;9:348. Epub 2018 Feb 27.
    State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, China.
    Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium . However, whether RovM regulates the urease activity in was still unknown. Read More

    The invasin D protein from selectively binds the Fab region of host antibodies and affects colonization of the intestine.
    J Biol Chem 2018 Jun 13;293(22):8672-8690. Epub 2018 Mar 13.
    From the Young Investigator Group Structural Biology of Autophagy, Department of Structure and Function of Proteins,
    is a Gram-negative bacterium and zoonotic pathogen responsible for a wide range of diseases, ranging from mild diarrhea, enterocolitis, lymphatic adenitis to persistent local inflammation. The invasin D (InvD) molecule belongs to the invasin (InvA)-type autotransporter proteins, but its structure and function remain unknown. In this study, we present the first crystal structure of InvD, analyzed its expression and function in a murine infection model, and identified its target molecule in the host. Read More

    Control of Heme Uptake Genes in in Response to Iron Sources.
    Front Cell Infect Microbiol 2018 22;8:47. Epub 2018 Feb 22.
    Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA, United States.
    Despite the mammalian host actively sequestering iron to limit pathogenicity, heme (or hemin when oxidized) and hemoproteins serve as important sources of iron for many bloodborne pathogens. The HmuRSTUV hemin uptake system allows species to uptake and utilize hemin and hemoproteins as iron sources. HmuR is a TonB-dependent outer membrane receptor for hemin and hemoproteins. Read More

    [Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical trap].
    Prikl Biokhim Mikrobiol 2017 Mar-Apr;53(2):234-43
    A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. Read More

    A Single Amino Acid Change in the Response Regulator PhoP, Acquired during Yersinia pestis Evolution, Affects PhoP Target Gene Transcription and Polymyxin B Susceptibility.
    J Bacteriol 2018 May 9;200(9). Epub 2018 Apr 9.
    Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA
    , the causative agent of plague, evolved from the closely related pathogen During its emergence, is believed to have acquired its unique pathogenic characteristics through numerous gene gains/losses, genomic rearrangements, and single nucleotide polymorphism (SNP) changes. One such SNP creates a single amino acid variation in the DNA binding domain of PhoP, the response regulator in the PhoP/PhoQ two-component system. and the basal human-avirulent strains of harbor glycines at position 215 of PhoP, whereas the modern human-virulent strains (e. Read More

    Loss of CNFY toxin-induced inflammation drives Yersinia pseudotuberculosis into persistency.
    PLoS Pathog 2018 02 1;14(2):e1006858. Epub 2018 Feb 1.
    Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    Gastrointestinal infections caused by enteric yersiniae can become persistent and complicated by relapsing enteritis and severe autoimmune disorders. To establish a persistent infection, the bacteria have to cope with hostile surroundings when they transmigrate through the intestinal epithelium and colonize underlying gut-associated lymphatic tissues. How the bacteria gain a foothold in the face of host immune responses is poorly understood. Read More

    Growth of Yersinia pseudotuberculosis Strains at Different Temperatures, pH Values, and NaCl and Ethanol Concentrations.
    J Food Prot 2018 Jan;81(1):142-149
    Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014 University of Helsinki, Helsinki, Finland (ORCID: [R.K.-T.]).
    Maximum growth temperature and growth limits in Luria-Bertani broth at different pH values and NaCl and ethanol concentrations were determined for 49 Yersinia pseudotuberculosis strains representing serotypes O:1, O:2, O:3, O:4, and O:5. In addition, the ability of the strains to grow at 0°C and the growth parameters at 1°C were determined. The maximum growth temperatures measured by Gradiplate temperature incubator varied between 42. Read More

    Effects of urbanization on host-pathogen interactions, using Yersinia in house sparrows as a model.
    PLoS One 2017 27;12(12):e0189509. Epub 2017 Dec 27.
    Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
    Urbanization strongly affects biodiversity, altering natural communities and often leading to a reduced species richness. Yet, despite its increasingly recognized importance, how urbanization impacts on the health of individual animals, wildlife populations and on disease ecology remains poorly understood. To test whether, and how, urbanization-driven ecosystem alterations influence pathogen dynamics and avian health, we use house sparrows (Passer domesticus) and Yersinia spp. Read More

    Crystal structure of d-glycero-α-d-manno-heptose-1-phosphate guanylyltransferase from Yersinia pseudotuberculosis.
    Biochim Biophys Acta 2018 03 23;1866(3):482-487. Epub 2017 Dec 23.
    College of Pharmacy, Ewha W. University, 52, Ewhayeodae-gil, Seoul, 03760, Republic of Korea. Electronic address:
    The Gram-negative bacterium Yersinia pseudotuberculosis is the causative agent of yersiniosis. d-glycero-α-d-manno-heptose-1-phosphate guanylyltransferase (HddC) is the fourth enzyme of the GDP-d-glycero-α-d-manno-heptose biosynthesis pathway which is important for the virulence of the microorganism. Therefore, HddC is a potential target of antibiotics against yersiniosis. Read More

    [Bile-resistant Gram-negative bacteria effect of different kinds of root decoction pieces].
    Zhongguo Zhong Yao Za Zhi 2017 Nov;42(21):4135-4141
    Medical School of Wuhan, University of Science and Technology, Wuhan 430065, China.
    To investigate the microbial contamination in Chinese herbal decoction pieces with different functional types by studying the total aerobic microbial count (TAMC), and total yeast and mould count (TYMC) in 40 samples of 8 types of root decoction pieces; further evaluate the contamination load of bile-resistant Gram-negative bacteria, and identify the Gram-negative bacteria by using biochemical identification system for Gram-negative bacteria. Our results showed that the TAMC value was more than 1 000 CFU•g⁻¹ in 85% (34/40) samples, and was more than 100 CFU•g⁻¹ in 30% (12/40) samples; the contamination of bile-resistant Gram-negative bacteria was detected in 45% (18/40) of the samples. The bile-resistant Gram-negative bacteria load of seven batches of samples was N>1 000 MPN•g⁻¹. Read More

    CCR2 Inflammatory Monocytes Are Recruited to Yersinia pseudotuberculosis Pyogranulomas and Dictate Adaptive Responses at the Expense of Innate Immunity during Oral Infection.
    Infect Immun 2018 Mar 20;86(3). Epub 2018 Feb 20.
    Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA
    Murine Ly6C inflammatory monocytes (IMs) require CCR2 to leave the bone marrow and enter mesenteric lymph nodes (MLNs) and other organs in response to infection. We are investigating how IMs, which can differentiate into CD11c dendritic cells (DCs), contribute to innate and adaptive immunity to Previously, we obtained evidence that IMs are important for a dominant CD8 T cell response to the epitope YopE and host survival using intravenous infections with attenuated Here we challenged CCR2 or CCR2 mice orally with wild-type to investigate how IMs contribute to immune responses during intestinal infection. Unexpectedly, CCR2 mice did not have reduced survival but retained body weight better and their MLNs cleared faster and with reduced lymphadenopathy compared to controls. Read More

    Self-Organization of Recombinant Membrane Porin OmpF from Yersinia pseudotuberculosis in Aqueous Environments.
    Biochemistry (Mosc) 2017 Nov;82(11):1304-1313
    Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
    Recombinant porin OmpF (an integral protein of bacterial outer membrane) from Yersinia pseudotuberculosis was synthesized in Escherichia coli cells as inclusion bodies. By combining the methods of anion-exchange and gel filtration chromatographies, recombinant OmpF (rOmpF) was isolated as an individual protein in its denatured state, and its characteristic properties (molecular mass, N-terminal amino acid sequence, and hydrodynamic radius of the protein in 8 M urea solution) were determined. According to the data of gel filtration, dynamic light scattering, optical spectroscopy, and binding of the hydrophobic fluorescent probe 8-anilino-1-naphthalenesulfonic acid, the rOmpF is fully unfolded in 8 M urea and exists in random coil conformation. Read More

    White Adipose Tissue Is a Reservoir for Memory T Cells and Promotes Protective Memory Responses to Infection.
    Immunity 2017 12 5;47(6):1154-1168.e6. Epub 2017 Dec 5.
    Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA; NIAID Microbiome Program, NIH, Bethesda, MD 20892, USA. Electronic address:
    White adipose tissue bridges body organs and plays a fundamental role in host metabolism. To what extent adipose tissue also contributes to immune surveillance and long-term protective defense remains largely unknown. Here, we have shown that at steady state, white adipose tissue contained abundant memory lymphocyte populations. Read More

    Effects of host cell sterol composition upon internalization of and clustered β1 integrin.
    J Biol Chem 2018 Jan 2;293(4):1466-1479. Epub 2017 Dec 2.
    From the Departments of Biochemistry and Cell Biology and
    is a foodborne pathogenic bacterium that causes acute gastrointestinal illness, but its mechanisms of infection are incompletely described. We examined how host cell sterol composition affected uptake. To do this, we depleted or substituted cholesterol in human MDA-MB-231 epithelial cells with various alternative sterols. Read More

    Bioluminescent tracing of a Yersinia pestis pCD1-mutant and Yersinia pseudotuberculosis in subcutaneously infected mice.
    Microbes Infect 2018 Mar 26;20(3):166-175. Epub 2017 Nov 26.
    Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. Electronic address:
    Yersinia pestis has evolved from Yersinia pseudotuberculosis serotype O:1b. A typical Y. pestis contains three plasmids: pCD1, pMT1 and pPCP1. Read More

    Phylogeographic separation and formation of sexually discrete lineages in a global population of .
    Microb Genom 2017 Oct 18;3(10):e000133. Epub 2017 Sep 18.
    Institute of Microbiology and Infection, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK.
    is a Gram-negative intestinal pathogen of humans and has been responsible for several nationwide gastrointestinal outbreaks. Large-scale population genomic studies have been performed on the other human pathogenic species of the genus and allowing a high-resolution understanding of the ecology, evolution and dissemination of these pathogens. However, to date no purpose-designed large-scale global population genomic analysis of has been performed. Read More

    Investigation of the Physical and Bioactive Properties of Bromo- and Iodo-Containing Sponge-Derived Compounds Possessing an Oxyphenylethanamine Core.
    J Nat Prod 2017 Dec 16;80(12):3255-3266. Epub 2017 Nov 16.
    Department of Chemistry and Biochemistry, University of California , Santa Cruz, California 95064, United States.
    This research set out to identify compounds from marine sponges that can act as bacterial virulence blockers. Extracts from a total of 80 sponges collected from throughout Indonesia were screened in a high-throughput NF-κB-based screen that identifies compounds capable of inhibiting the bacterial type III secretion system (T3SS) in Yersinia pseudotuberculosis. An extract that was shown to inhibit T3SS-driven NF-κB expression was obtained from an Iotrochota cf. Read More

    ArfGAP1 restricts entry by controlling the actin cytoskeleton.
    EMBO Rep 2018 Jan 15;19(1):29-42. Epub 2017 Nov 15.
    CNRS, Inserm, CHU Lille, U1019 - UMR8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Univ. Lille, Lille, France
    The interaction of (Mtb) with pulmonary epithelial cells is critical for early stages of bacillus colonization and during the progression of tuberculosis. Entry of Mtb into epithelial cells has been shown to depend on F-actin polymerization, though the molecular mechanisms are still unclear. Here, we demonstrate that mycobacterial uptake into epithelial cells requires rearrangements of the actin cytoskeleton, which are regulated by ADP-ribosylation factor 1 (Arf1) and phospholipase D1 (PLD1), and is dependent on the M3 muscarinic receptor (MR). Read More

    Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene.
    Braz J Microbiol 2018 Apr - Jun;49(2):320-328. Epub 2017 Oct 18.
    Tianjin University, School of Life Sciences, Tianjin, China. Electronic address:
    Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. Read More

    Multiple factors correlating with wing malformations in the population of Parnassius apollo (Lepidoptera: Papilionidae) restituted from a low number of individuals: A mini review.
    Insect Sci 2017 Nov 2. Epub 2017 Nov 2.
    Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
    The Apollo butterfly, Parnassius apollo (Linnaeus), was common in Europe over 100 years ago, but currently it is considered as near threatened. Different conservation programs have promoted the persistence of this species; however, it is still endangered. An example of such programs was the action devoted to reestablish the Apollo butterfly population in Pieniny National Park (Poland) from only 20-30 individuals which had survived till the last decade of the 20th century. Read More

    Far East Scarlet-like Fever Masquerading as Adult-onset Kawasaki Disease.
    Intern Med 2018 Feb 1;57(3):437-440. Epub 2017 Nov 1.
    Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Japan.
    A previously healthy 31-year-old man was referred to us with refractory septic shock accompanied by bilateral conjunctival congestion and erythema of his right lower limb. Nine days after admission, he had bilateral desquamation of the fingertips, and his presentation satisfied the criteria for Kawasaki disease. A serological examination was positive for Yersinia pseudotuberculosis, and he was diagnosed with Far East scarlet-like fever (FESLF). Read More

    In silico and in vitro analysis of cross-reactivity between Yersinia pseudotuberculosis OmpF porin and thyroid-stimulating hormone receptor.
    Int J Biol Macromol 2018 Feb 25;107(Pt B):2484-2491. Epub 2017 Oct 25.
    G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, Vladivostok, 690022, Russia.
    This work is devoted to the ascertainment of serological cross-reactivity between OmpF porin from Yersinia pseudotuberculosis (YpOmpF) and human thyroid-stimulating hormone receptor (hTSHR). Extracts containing hTSHR were isolated from surgical thyroid tissue of patients with clinical and diagnostic signs of diffuse toxic goiter. Monoclonal antibodies to hTSHR (mAbs) were shown to interact both with antigens in thyroid tissue extracts and with YpOmpF. Read More

    The Product of Yersinia pseudotuberculosis mcc Operon Is a Peptide-Cytidine Antibiotic Activated Inside Producing Cells by the TldD/E Protease.
    J Am Chem Soc 2017 11 31;139(45):16178-16187. Epub 2017 Oct 31.
    Institute of Gene Biology , Russian Academy of Science, 34/5 Vavilov str., 119334 Moscow, Russia.
    Microcin C is a heptapeptide-adenylate antibiotic produced by some strains of Escherichia coli. Its peptide part is responsible for facilitated transport inside sensitive cells where it is proteolyzed with release of a toxic warhead-a nonhydrolyzable aspartamidyl-adenylate, which inhibits aspartyl-tRNA synthetase. Recently, a microcin C homologue from Bacillus amyloliquefaciens containing a longer peptide part modified with carboxymethyl-cytosine instead of adenosine was described, but no biological activity of this compound was revealed. Read More

    The impact of length variations in the L2 loop on the structure and thermal stability of non-specific porins: The case of OmpCs from the Yersinia pseudotuberculosis complex.
    Biochim Biophys Acta 2018 02 14;1860(2):515-525. Epub 2017 Oct 14.
    G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, 690022 Prospect 100-let Vladivostoku 159, Vladivostok, Russia. Electronic address:
    Porins are integral proteins of the outer membranes of gram-negative bacteria. In membranes, they exist as homotrimers and the L2 loops contribute to their stability. Comparison of OmpC porins of the Yersinia pseudotuberculosis complex with other enterobacterial porins demonstrated L2 loop length diversity, which is caused by varying numbers of dipeptide/tripeptide repeats. Read More

    Multiple Small Intestine Ulcers with Desquamation of the Fingers.
    Case Rep Gastroenterol 2017 May-Aug;11(2):452-461. Epub 2017 Aug 17.
    Department of Gastroenterology, Ishikawa Prefectural Central Hospital, Kanazawa, Japan.
    A 73-year-old female was admitted to our hospital with abdominal pain and diarrhea. Computed tomography detected distension of the small intestine. A palmar erythema, multiple oral ulcers, and desquamation of the fingers appeared after hospitalization. Read More

    Plasmid-mediated doxycycline resistance in a Yersinia pestis strain isolated from a rat.
    Int J Antimicrob Agents 2018 Feb 10;51(2):249-254. Epub 2017 Oct 10.
    Yersinia Research Unit, WHO Collaborating Center, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France. Electronic address:
    The emergence of antibiotic-resistant Yersinia pestis strains represents a public health concern. Two antibiotic-resistant Y. pestis strains isolated from Madagascar have been previously identified and characterised. Read More

    Differential impact of lipopolysaccharide defects caused by loss of RfaH in Yersinia pseudotuberculosis and Yersinia pestis.
    Sci Rep 2017 Sep 7;7(1):10915. Epub 2017 Sep 7.
    Department of Microbiology and Molecular Biology, 4007 LSB, Brigham Young University, Provo, UT, 84602, USA.
    RfaH enhances transcription of a select group of operons controlling bacterial surface features such as lipopolysaccharide (LPS). Previous studies have suggested that rfaH may be required for Yersinia pseudotuberculosis resistance to antimicrobial chemokines and survival during mouse infections. In order to further investigate the role of RfaH in LPS synthesis, resistance to host defense peptides, and virulence of Yersinia, we constructed ΔrfaH mutants of Y. Read More

    RIPK1-dependent apoptosis bypasses pathogen blockade of innate signaling to promote immune defense.
    J Exp Med 2017 Nov 30;214(11):3171-3182. Epub 2017 Aug 30.
    Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA
    Many pathogens deliver virulence factors or effectors into host cells in order to evade host defenses and establish infection. Although such effector proteins disrupt critical cellular signaling pathways, they also trigger specific antipathogen responses, a process termed "effector-triggered immunity." The Gram-negative bacterial pathogen inactivates critical proteins of the NF-κB and MAPK signaling cascade, thereby blocking inflammatory cytokine production but also inducing apoptosis. Read More

    Oral delivery of Escherichia coli persistently infected with M2e-displaying bacteriophages partially protects against influenza A virus.
    J Control Release 2017 Oct 24;264:55-65. Epub 2017 Aug 24.
    VIB-UGent Center for Medical Biotechnology, VIB, Technologiepark, 927, Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, Technologiepark 927, Ghent, Belgium. Electronic address:
    We describe a novel live oral vaccine type. Conceptually, this vaccine is based on a non-lytic, recombinant filamentous bacteriophage that displays an antigen of interest. To provide proof of concept we used the amino-terminal part of a conserved influenza A virus epitope, i. Read More

    C4B gene influences intestinal microbiota through complement activation in patients with paediatric-onset inflammatory bowel disease.
    Clin Exp Immunol 2017 Dec 25;190(3):394-405. Epub 2017 Sep 25.
    Immunobiology, Research Programs Unit, University of Helsinki, Helsinki, Finland.
    Complement C4 genes are linked to paediatric inflammatory bowel disease (PIBD), but the mechanisms have remained unclear. We examined the influence of C4B gene number on intestinal microbiota and in-vitro serum complement activation by intestinal microbes in PIBD patients. Complement C4A and C4B gene numbers were determined by genomic reverse transcription-polymerase chain reaction (RT-PCR) from 64 patients with PIBD (Crohn's disease or ulcerative colitis). Read More

    Molecular bases for multidrug resistance in Yersinia pseudotuberculosis.
    Int J Med Microbiol 2017 Oct 16;307(7):371-381. Epub 2017 Aug 16.
    Yersinia Research Unit and Yersinia National Reference Laboratory, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris cedex 15, France. Electronic address:
    The enteropathogen Yersinia pseudotuberculosis causes gastrointestinal infections in humans. Although this species is usually susceptible to antibiotics active against Gram-negative bacteria, we identified three multidrug resistant (MDR) strains of Y. pseudotuberculosis that were isolated from the environment in Russia and from a patient in France. Read More

    Guanylate Binding Proteins Regulate Inflammasome Activation in Response to Hyperinjected Yersinia Translocon Components.
    Infect Immun 2017 10 20;85(10). Epub 2017 Sep 20.
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Gram-negative bacterial pathogens utilize virulence-associated secretion systems to inject, or translocate, effector proteins into host cells to manipulate cellular processes and promote bacterial replication. However, translocated bacterial products are sensed by ucleotide binding domain and eucine-rich epeat-containing proteins (NLRs), which trigger the formation of a multiprotein complex called the inflammasome, leading to secretion of interleukin-1 (IL-1) family cytokines, pyroptosis, and control of pathogen replication. Pathogenic bacteria inject effector proteins termed Yops, as well as pore-forming proteins that comprise the translocon itself, into target cells. Read More

    Engineering of Chimeric Protein Based on E Protein Domain III of Tick- Borne Encephalitis Virus and OmpF Porin of Yersinia pseudotuberculosis.
    Protein Pept Lett 2017 ;24(10):974-981
    Far Eastern Federal University, Vladivostok. Russian Federation.
    Background: Tick-borne encephalitis poses a serious public health threat in the endemic regions. The disease treatment is restricted to symptomatic therapy, so great expectations are in the development of the prophylactic and therapeutic vaccines. The domain III of E protein of the tickborne encephalitis virus is the main antigenic domain which includes virus-specific epitopes recognized by neutralizing antibodies. Read More

    Development of a novel multiplexed qPCR and Pyrosequencing method for the detection of human pathogenic yersiniae.
    Int J Food Microbiol 2017 Sep 20;257:247-253. Epub 2017 Jun 20.
    Canadian Food Inspection Agency, Lethbridge Laboratory, Township Rd 9-1, Lethbridge, Alberta T1J 3Z4, Canada. Electronic address:
    The purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. Yersinia enterocolitica, Y. Read More

    Synthetic Cyclic Peptomers as Type III Secretion System Inhibitors.
    Antimicrob Agents Chemother 2017 Sep 24;61(9). Epub 2017 Aug 24.
    Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, California, USA
    Antibiotic-resistant bacteria are an emerging threat to global public health. New classes of antibiotics and tools for antimicrobial discovery are urgently needed. Type III secretion systems (T3SS), which are required by dozens of Gram-negative bacteria for virulence but largely absent from nonpathogenic bacteria, are promising virulence blocker targets. Read More

    Epigallocatechin gallate inhibits the type III secretion system of Gram-negative enteropathogenic bacteria under model conditions.
    FEMS Microbiol Lett 2017 Jul;364(13)
    Department of Bacteriology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
    Epigallocatechin gallate (EGCG), a major polyphenol in green tea, inhibits the type III secretion system (T3SS) of enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), Salmonella enterica serovar Typhimurium, and Yersinia pseudotuberculosis. The inhibitory effect causes the inhibition of hemolysis, cell invasion, cell adhesion and apoptosis, which are functions of the type III secretion device. In the case of EPEC, EspB accumulates in the cells. Read More

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