3,714 results match your criteria Pseudotuberculosis Yersinia


Changes in Transcriptome of IP32953 Grown at 3 and 28°C Detected by RNA Sequencing Shed Light on Cold Adaptation.

Front Cell Infect Microbiol 2018 27;8:416. Epub 2018 Nov 27.

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.

is a bacterium that not only survives, but also thrives, proliferates, and remains infective at cold-storage temperatures, making it an adept foodborne pathogen. We analyzed the differences in gene expression between IP32953 grown at 3 and 28°C to investigate which genes were significantly more expressed at low temperature at different phases of growth. We isolated and sequenced the RNA from six distinct corresponding growth points at both temperatures to also outline the expression patterns of the differentially expressed genes. Read More

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November 2018

The polyanions heparin and suramin impede binding of free adenine to a DNA glycosylase from C. pseudotuberculosis.

Int J Biol Macromol 2018 Dec 7. Epub 2018 Dec 7.

Multiuser Center for Biomolecular Innovation, Department of Physics, Instituto de Biociências Letras e Ciências Exatas (Ibilce), Universidade Estadual Paulista (UNESP), São Jose do Rio Preto, SP, 15054-000, Brazil. Electronic address:

Currently no effective treatment is available to combat infections caused by Corynebacterium pseudotuberculosis in livestock. Survival of this Gram-positive bacterium in rapidly-growing pathogens in hostile environments is strongly dependent on the existence of a robust DNA repair system to prevent DNA mutations and contribute to bacterial colonization and virulence. The adenine/guanine-specific DNA glycosylase (MutY) is evolutionarily conserved and has been well characterized due to its central role in the prevention of mutagenesis and DNA repair. Read More

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December 2018

The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.

Virulence 2018 Dec 5. Epub 2018 Dec 5.

a Department of Molecular Biology , Umeå University , Umeå , Sweden.

The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Read More

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December 2018
2 Reads

Identification of specific sequence motif of YopN of Yersinia pseudotuberculosis required for systemic infection.

Virulence 2019 Dec;10(1):10-25

a Department of Molecular Biology, Umeå Centre for Microbial Research UCMR , Umeå University , Umeå , Sweden.

Type III secretion systems (T3SSs) are tightly regulated key virulence mechanisms shared by many Gram-negative pathogens. YopN, one of the substrates, is also crucial in regulation of expression, secretion and activation of the T3SS of pathogenic Yersinia species. Interestingly, YopN itself is also targeted into host cells but so far no activity or direct role for YopN inside host cells has been described. Read More

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December 2019

Humoral and cellular immune correlates of protection against bubonic plague by a live Yersinia pseudotuberculosis vaccine.

Vaccine 2019 Jan 19;37(1):123-129. Epub 2018 Nov 19.

Unité de Recherche Yersinia, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France. Electronic address:

Immunization with the live-attenuated Yersinia pseudotuberculosis VTnF1 strain producing a Yersinia pestis F1 pseudocapsule efficiently protects mice against bubonic and pneumonic plague. In clinical trials, demonstration of a plague vaccine's efficacy in humans will not be feasible, and correlates of protection will be needed to bridge the immune response of protected animals to that of vaccinated humans. Using serum transfer and vaccination of antibody-deficient µMT mice, we established that both humoral and cellular responses elicited by VTnF1 independently conferred protection against bubonic plague. Read More

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January 2019
4 Reads

Discovering RNA-Based Regulatory Systems for Virulence.

Front Cell Infect Microbiol 2018 25;8:378. Epub 2018 Oct 25.

Department of Molecular Infection Biology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

The genus includes three human pathogenic species, , the causative agent of the bubonic and pneumonic plague, and enteric pathogens and that cause a number of gut-associated diseases. Over the past years a large repertoire of RNA-based regulatory systems has been discovered in these pathogens using different RNA-seq based approaches. Among them are several conserved or species-specific RNA-binding proteins, regulatory and sensory RNAs as well as various RNA-degrading enzymes. Read More

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October 2018
3 Reads

Seroepidemiological characterization and risk factors associated with seroconversion to Corynebacterium pseudotuberculosis in goats from Northeastern Brazil.

Trop Anim Health Prod 2018 Nov 12. Epub 2018 Nov 12.

Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, Patos, PB, Brazil.

Goat breeding in the Northeast region of Brazil plays an important socioeconomic role. However, there are significant losses caused by sanitary deficits and infectious diseases, particularly caseous lymphadenitis (CL). Although CL is considered endemic in Northeastern Brazil, a comprehensive and up-to-date study of this disease in goat herds in this region is necessary. Read More

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November 2018
3 Reads

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis.

PLoS One 2018 12;13(11):e0207304. Epub 2018 Nov 12.

Department of General Biology, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

Phylogenomics and genome scale positive selection analyses were performed on 29 Corynebacterium pseudotuberculosis genomes that were isolated from different hosts, including representatives of the Ovis and Equi biovars. A total of 27 genes were identified as undergoing adaptive changes. An analysis of the clades within this species and these biovars, the genes specific to each branch, and the genes responding to selective pressure show clear differences, indicating that adaptation and specialization is occurring in different clades. Read More

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November 2018
1 Read

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged.

Vet World 2018 Sep 17;11(9):1272-1276. Epub 2018 Sep 17.

Department of Microbiology and Immunology, National Research Centre, Dokki, Cairo, Egypt.

Background: Caseous lymphadenitis (CLA) is a serious disease affects sheep and goat, caused by . Due to it is non-treatable disease, so the effective preventive vaccines are considered a significant way to combat the disease. All strains of have several virulence factors that associated with their cell invasion, survival, and proliferation such as phospholipase D (PLD), outer lipid coat, and secreted proteases. Read More

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September 2018
3 Reads

Caspase-8 induces cleavage of gasdermin D to elicit pyroptosis during infection.

Proc Natl Acad Sci U S A 2018 11 31;115(46):E10888-E10897. Epub 2018 Oct 31.

Department of Immunology, Tufts University School of Medicine, Boston, MA 02111;

Cell death and inflammation are intimately linked during infection. Pathogenic inhibits the MAP kinase TGFβ-activated kinase 1 (TAK1) via the effector YopJ, thereby silencing cytokine expression while activating caspase-8-mediated cell death. Here, using in corroboration with costimulation of lipopolysaccharide and (5Z)-7-Oxozeaenol, a small-molecule inhibitor of TAK1, we show that caspase-8 activation during TAK1 inhibition results in cleavage of both gasdermin D (GSDMD) and gasdermin E (GSDME) in murine macrophages, resulting in pyroptosis. Read More

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November 2018
6 Reads

Systemic Yersinia pseudotuberculosis as a Cause of Osteomyelitis in a Captive Ring-tailed Lemur (Lemur catta).

J Comp Pathol 2018 Oct 11;164:27-31. Epub 2018 Sep 11.

Royal (Dick) School of Veterinary Studies, The Roslin Institute, University of Edinburgh, Easter Bush, Edinburgh, UK.

Yersinia pseudotuberculosis and Yersinia enterocolitica are ubiquitous pathogens with wildlife and domestic animal reservoirs. Outbreaks of 'non-plague' yersiniosis in man and non-human primates are reported frequently (including zoological specimens and research breeding colonies) and are usually characterized by enteritis, mesenteric lymphadenitis and occasionally organ abscessation. In people, non-septic reactive arthritis is a common sequela to yersiniosis. Read More

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October 2018
1 Read

exploits CD209 receptors for promoting host dissemination and infection.

Infect Immun 2018 Oct 22. Epub 2018 Oct 22.

Department of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, Hubei, China

is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade into the small intestine via the Peyer's patches to initiate dissemination. Read More

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October 2018
6 Reads
3.730 Impact Factor

First genome sequencing and comparative analyses of strains from Mexico.

Stand Genomic Sci 2018 10;13:21. Epub 2018 Oct 10.

1Laboratory of Cellular and Molecular Genetics, Institute of Biologic Sciences, Federal University of Minas Gerais, Belo Horizonte, MG Brazil.

is a pathogenic bacterium which has been rapidly spreading all over the world, causing economic losses in the agricultural sector and sporadically infecting humans. Six strains were isolated from goats, sheep, and horses with distinct abscess locations. For the first time, Mexican genomes of this bacterium were sequenced and studied in silico. Read More

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October 2018
2 Reads

Prevalence of Species in the Ileum of Crohn's Disease Patients and Controls.

Front Cell Infect Microbiol 2018 21;8:336. Epub 2018 Sep 21.

UMR1149 INSERM, Research Centre on Inflammation, Université Paris Diderot-Sorbonne Paris-Cité, Paris, France.

are common contaminants of food products, but their prevalence in the human gut is poorly documented. have been implicated in Crohn's Disease (CD, an inflammatory bowel disease) however their role in CD is controversial. We performed highly sensitive PCR assays of specific sequences for the gene of and the gene of . Read More

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September 2018
3 Reads
2.620 Impact Factor

BarA-UvrY Two-Component Regulatory System Represses Biofilms via CsrB.

Front Cell Infect Microbiol 2018 18;8:323. Epub 2018 Sep 18.

Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States.

The formation of biofilms by () and requires the genes, which direct production of a polysaccharide extracellular matrix (Hms-ECM). Despite possessing identical sequences, produces much less Hms-ECM than . The regulatory influences that control Hms-ECM production and biofilm formation are not fully understood. Read More

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September 2018
9 Reads

Immunogenicity and Protective Activity of a Chimeric Protein Based on the Domain III of the Tick-Borne Encephalitis Virus E Protein and the OmpF Porin of Incorporated into the TI-Complex.

Int J Mol Sci 2018 Sep 30;19(10). Epub 2018 Sep 30.

Far Eastern Federal University, 8 Sukhanova St., Vladivostok 690091, Russia.

Tick-borne encephalitis (TBE) is a widespread, dangerous infection. Unfortunately, all attempts to create safe anti-TBE subunit vaccines are still unsuccessful due to their low immunogenicity. The goal of the present work was to investigate the immunogenicity of a recombinant chimeric protein created by the fusion of the EIII protein, comprising domain III and a stem region of the tick-borne encephalitis virus (TBEV) E protein, and the OmpF porin of (OmpF-EIII). Read More

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September 2018
3 Reads

Ail provides multiple mechanisms of serum resistance to Yersinia pestis.

Mol Microbiol 2018 Sep 27. Epub 2018 Sep 27.

Division of Integrated Biomedical Sciences, University of Detroit Mercy School of Dentistry, Detroit, MI, USA.

Ail, a multifunctional outer membrane protein of Yersinia pestis, confers cell binding, Yop delivery and serum resistance activities. Resistance to complement proteins in serum is critical for the survival of Y. pestis during the septicemic stage of plague infections. Read More

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September 2018
24 Reads

The Cytotoxic Necrotizing Factor of Yersinia pseudotuberculosis (CNFy) is Carried on Extracellular Membrane Vesicles to Host Cells.

Sci Rep 2018 Sep 21;8(1):14186. Epub 2018 Sep 21.

School of Life Sciences, Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan, South Korea.

In this study we show Yersinia pseudotuberculosis secretes membrane vesicles (MVs) that contain different proteins and virulence factors depending on the strain. Although MVs from Y. pseudotuberculosis YPIII and ATCC 29833 had many proteins in common (68. Read More

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September 2018
1 Read

Novel diagnostic ELISA test for discrimination between infections with Yersinia enterocolitica and Yersinia pseudotuberculosis.

Eur J Clin Microbiol Infect Dis 2018 Dec 20;37(12):2301-2306. Epub 2018 Sep 20.

Omnio AB, Umeå, Sweden.

Yersiniosis is a foodborne infection caused by Yersinia enterocolitica or Yersinia pseudotuberculosis. Although yersiniosis is most often self-limiting, some patients develop chronic infections, such as reactive arthritis, glomerulonephritis, or myocarditis, which require an antibiotic treatment. Whereas early infections can be diagnosed by direct detection of bacteria, chronic infections can only be identified by serological tests. Read More

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December 2018
1 Read

Assessment of the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis in young Merino sheep.

N Z Vet J 2019 Jan 3;67(1):27-35. Epub 2018 Oct 3.

a Faculty of Veterinary and Agricultural Sciences , University of Melbourne , 250 Princes Highway, Werribee , VIC , Australia.

AIMS To assess the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis III in preventing clinical disease and deaths due to yersiniosis in young Merino sheep, and to determine the effect of vaccination on the prevalence of faecal shedding of pathogenic Yersinia spp., daily liveweight gain, and development of antibodies to Yersinia spp. following vaccination and natural exposure. Read More

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January 2019
2 Reads

Caseous Lymphadenitis Caused by Corynebacterium pseudotuberculosis in Alpine Chamois (Rupicapra r. rupicapra): a Review of 98 Cases.

J Comp Pathol 2018 May 1;161:11-19. Epub 2018 Jun 1.

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Valle d'Aosta Department, National Reference Centre for Wildlife Diseases, Regione Amerique 7G, Quart, Italy.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) in domestic and wild ruminants. Here we describe CLA in alpine chamois (Rupicapra r. rupicapra) based on a series of 98 cases of C. Read More

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May 2018
1 Read

Seroprevalence of antibodies against bacterial pathogens in sheep from Equatorial Guinea.

Rev Sci Tech 2017 Dec;36(3):965-970

Equatorial Guinea (EG) is a country in Central Africa with typical tropical weather. Sheep are an important source of food in EG, but the absence of information regarding infectious diseases that affect the native flocks of small ruminants is a concern. The country is currently implementing several new practices in the sheep industry associated with the importation of sheep from other countries. Read More

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December 2017
9 Reads

Recombinant Fusion Protein Joining E Protein Domain III of Tick-Borne Encephalitis Virus and HSP70 of as an Antigen for the TI-Complexes.

Biomolecules 2018 Aug 25;8(3). Epub 2018 Aug 25.

Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University, Sukhanov St., 8, Vladivostok 690091, Russia.

Domain III (DIII) of the tick-borne encephalitis virus (TBEV) protein E contains epitopes, which induce antibodies capable of neutralizing the virus. To enhance the immunogenicity of this protein, which has a low molecular weight, the aim of the present work was to express, isolate, and characterize a chimeric protein based on the fusion of the bacterial chaperone HSP70 of and EIII (DIII + stem) as a prospective antigen for an adjuvanted delivery system, the tubular immunostimulating complex (TI-complex). The chimeric construction was obtained using pET-40b(+) vector by ligating the respective genes. Read More

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August 2018
3 Reads

LFchimera protects HeLa cells from invasion by Yersinia spp. in vitro.

Biometals 2018 Dec 22;31(6):941-950. Epub 2018 Aug 22.

Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.

Yersinia pestis is the causative agent of plague. As adequate antibiotic treatment falls short and currently no effective vaccine is available, alternative therapeutic strategies are needed. In order to contribute to solving this problem we investigated the therapeutic potential of the peptide construct LFchimera against the safer-to-handle Y. Read More

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December 2018
9 Reads

All Are Not Created Equal: Phenotypic Adaptation to Distinct Niches Within Mammalian Tissues.

Authors:
Kimberly M Davis

Front Cell Infect Microbiol 2018 3;8:261. Epub 2018 Aug 3.

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.

replicates within mammalian tissues to form clustered bacterial replication centers, called microcolonies. A subset of bacterial cells within microcolonies interact directly with host immune cells, and other subsets of bacteria only interact with other bacteria. This establishes a system where subsets of have distinct gene expression profiles, which are driven by their unique microenvironments and cellular interactions. Read More

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August 2018
1 Read

Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose.

Nature 2018 09 15;561(7721):122-126. Epub 2018 Aug 15.

National Institute of Biological Sciences, Beijing, China.

Immune recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors often activates proinflammatory NF-κB signalling. Recent studies indicate that the bacterial metabolite D-glycero-β-D-manno-heptose 1,7-bisphosphate (HBP) can activate NF-κB signalling in host cytosol, but it is unclear whether HBP is a genuine PAMP and the cognate pattern recognition receptor has not been identified. Here we combined a transposon screen in Yersinia pseudotuberculosis with biochemical analyses and identified ADP-β-D-manno-heptose (ADP-Hep), which mediates type III secretion system-dependent NF-κB activation and cytokine expression. Read More

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September 2018
3 Reads

Type VI Secretion Systems Present New Insights on Pathogenic .

Front Cell Infect Microbiol 2018 31;8:260. Epub 2018 Jul 31.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, China.

The type VI secretion system (T6SS) is a versatile secretion system widely distributed in Gram-negative bacteria that delivers multiple effector proteins into either prokaryotic or eukaryotic cells, or into the extracellular milieu. T6SS participates in various physiological processes including bacterial competition, host infection, and stress response. Three pathogenic species, namely , and , possess different copies of T6SSs with distinct biological functions. Read More

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July 2018
8 Reads

Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens.

Chembiochem 2018 Nov 11;19(21):2283-2292. Epub 2018 Oct 11.

Institut für Pharmazeutische Wissenschaften, Albert-Ludwigs-Universität Freiburg, Albertstrasse 25, 79104, Freiburg, Germany.

A wide range of thiamine diphosphate (ThDP)-dependent enzymes catalyze the benzoin-type carboligation of pyruvate with aldehydes. A few ThDP-dependent enzymes, such as YerE from Yersinia pseudotuberculosis (YpYerE), are known to accept ketones as acceptor substrates. Catalysis by YpYerE gives access to chiral tertiary alcohols, a group of products difficult to obtain in an enantioenriched form by other means. Read More

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November 2018
14 Reads

Transcriptome analysis of Corynebacterium pseudotuberculosis biovar Equi in two conditions of the environmental stress.

Gene 2018 Nov 8;677:349-360. Epub 2018 Aug 8.

Laboratory of Genomic and Bioinformatics, Center of Genomics and System Biology, Institute of Biological Science, Federal University of Para, Belém, Pará, Brazil, Rua Augusto Corrêa, Belém 66.075-110, Brazil. Electronic address:

Corynebacterium pseudotuberculosis has been widely studied in an effort to understand its biological evolution. Transcriptomics has revealed possible candidates for virulence and pathogenicity factors of strain 1002 (biovar Ovis). Because C. Read More

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November 2018
2 Reads
2.140 Impact Factor

Formation of a Secretion-Competent Protein Complex by a Dynamic Wrap-around Binding Mechanism.

J Mol Biol 2018 Sep 2;430(18 Pt B):3157-3169. Epub 2018 Aug 2.

Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden. Electronic address:

Bacterial virulence is typically initiated by translocation of effector or toxic proteins across host cell membranes. A class of gram-negative pathogenic bacteria including Yersinia pseudotuberculosis and Yersinia pestis accomplishes this objective with a protein assembly called the type III secretion system. Yersinia effector proteins (Yop) are presented to the translocation apparatus through formation of specific complexes with their cognate chaperones (Syc). Read More

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September 2018
4 Reads

Influence of Specific Bacteriophage on the Level of Vesicle Formation and Morphology of Cells of Yersinia pseudotuberculosis.

Bull Exp Biol Med 2018 Jul 13. Epub 2018 Jul 13.

Institute of Physiology, Komi Research Center, Ural Division of Russia Academy of Science, Vyatka State University, Kirov, Russia.

Incubation of Yersinia pseudotuberculosis cells grown on a solid medium with pseudotuberculous diagnostic bacteriophage for 20 min at 37oC led to a significant decrease in the concentration of both components of the system. This effect was absent when the bacteria were grown in a fluid medium. At the same time, this incubation regimen promoted vesicle formation and typical morphological changes in bacteria grown in both surface and suspension cultures. Read More

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July 2018
4 Reads

Yersinia pseudotuberculosis Prevalence and Diversity in Wild Boars in Northeast Germany.

Appl Environ Microbiol 2018 Sep 31;84(18). Epub 2018 Aug 31.

German Federal Institute for Risk Assessment, Department of Biological Safety, Berlin, Germany

In this study, the prevalence of in wild boars in northeast Germany was determined. For that purpose, the tonsils of 503 wild boars were sampled. The presence of was studied by diagnostic PCR. Read More

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September 2018
5 Reads

Investigation of Yersinia pestis and Yersinia pseudotuberculosis strains from Georgia and neighboring countries in the Caucasus by high-density SNP microarray.

Arch Microbiol 2018 Nov 4;200(9):1345-1355. Epub 2018 Jul 4.

Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Mailstop 70A3317, Berkeley, CA, 94720, USA.

Yersinia pestis, the causative agent of plague, is a recently evolved clone of the enteropathogenic bacterium Yersinia pseudotuberculosis. Y. pestis has been extensively studied for decades; however, there are insufficient data about the intra-species diversity of this microorganism in certain parts of the world, including the Caucasus region. Read More

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November 2018
5 Reads

Distribution of enteropathogenic Yersinia spp. and Salmonella spp. in the Swedish wild boar population, and assessment of risk factors that may affect their prevalence.

Acta Vet Scand 2018 Jul 3;60(1):40. Epub 2018 Jul 3.

Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, 750 07, Uppsala, Sweden.

Background: Pure Eurasian wild boars and/or hybrids with domestic pigs are present in the wild on most continents. These wild pigs have been demonstrated to carry a large number of zoonotic and epizootic pathogens such as Salmonella spp., Yersinia enterocolitica and Y. Read More

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July 2018
13 Reads

Leader gene of Corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach.

J Vet Med Sci 2018 Aug 25;80(8):1317-1324. Epub 2018 Jun 25.

Research and Development Laboratory of Vallée S.A., Montes Claros, Minas Gerais, 39400-000, Brazil.

We conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protein sequences collected from the NCBI GenPept database and the keywords "caseous lymphadenitis" and "Corynebacterium pseudotuberculosis" and "goats". A network was developed using the STRING 10 database, with a confidence score of 0. Read More

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August 2018
7 Reads

Wzx flippases exhibiting complex O-unit preferences require a new model for Wzx-substrate interactions.

Microbiologyopen 2018 Jun 10:e00655. Epub 2018 Jun 10.

School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales, Australia.

The Wzx flippase is a critical component of the O-antigen biosynthesis pathway, being responsible for the translocation of oligosaccharide O units across the inner membrane in Gram-negative bacteria. Recent studies have shown that Wzx has a strong preference for its cognate O unit, but the types of O-unit structural variance that a given Wzx can accommodate are poorly understood. In this study, we identified two Yersinia pseudotuberculosis Wzx that can distinguish between different terminal dideoxyhexose sugars on a common O-unit main-chain, despite both being able to translocate several other structurally-divergent O units. Read More

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June 2018
7 Reads

Inhibition of thioredoxin A1 from Corynebacterium pseudotuberculosis by polyanions and flavonoids.

Int J Biol Macromol 2018 Oct 7;117:1066-1073. Epub 2018 Jun 7.

Multiuser Center for Biomolecular Innovation, Department of Physics, Instituto de Biociências Letras e Ciências Exatas (Ibilce), Universidade Estadual Paulista (UNESP), São Jose do Rio Preto, SP 15054-000, Brazil. Electronic address:

In pathogens, the thioredoxin system forms part of the defense against oxidative stress and ensures the formation of the proper disulfide bonds to ensure protein function. In Corynebacterium pseudotuberculosis, the role and mechanism of TrxA1 has not been elucidated, but, the significant homology among different Trxs and the conservation of the residues that form their active sites underline the importance of the Trx systems. Proteins involved in redox metabolism and low molecular weight thiols, which might interact with them, become attractive targets to modulate the activity of pathogens. Read More

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October 2018
6 Reads

Grasping the nettle: A bacterial invasin that targets immunoglobulin variable domains.

Authors:
Paul Barlow

J Biol Chem 2018 Jun;293(22):8691-8692

From the Schools of Chemistry and Biological Sciences, University of Edinburgh, Edinburgh EH9 3FJ, Scotland, United Kingdom.

In a new paper, the protein InvD from , a zoonotic pathogen, is shown to assist late-stage invasion of intestinal epithelia. Remarkably, InvD acts by binding the Fab region of IgG or IgA. It straddles adjacent light-chain and heavy-chain variable domains, but its binding is different from that of antigens in that complementarity-determining regions do not participate. Read More

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High-throughput analysis of Yersinia pseudotuberculosis gene essentiality in optimised in vitro conditions, and implications for the speciation of Yersinia pestis.

BMC Microbiol 2018 May 31;18(1):46. Epub 2018 May 31.

Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.

Background: Yersinia pseudotuberculosis is a zoonotic pathogen, causing mild gastrointestinal infection in humans. From this comparatively benign pathogenic species emerged the highly virulent plague bacillus, Yersinia pestis, which has experienced significant genetic divergence in a relatively short time span. Much of our knowledge of Yersinia spp. Read More

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May 2018
7 Reads

In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence.

AMB Express 2018 May 30;8(1):89. Epub 2018 May 30.

Department of Biointeração-Health Sciences Institute, Federal University of Bahia, Salvador, Brazil.

Caseous lymphadenitis (LC) is a chronic contagious disease caused by Corynebacterium pseudotuberculosis, which mainly affects goats and sheep. Vaccination is an effective but not yet well-established method, partly due to a lack of knowledge surrounding the most effective immunoprotective components. The present study aimed to quantify and compare the in vivo expression of genes pld (phospholipase D), cpp (CP40), nanH (neuraminidase H), sodC (superoxide dismutase C) and spaC (adhesin) using qRT-PCR, with the respective expression in vitro. Read More

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May 2018
1 Read

The development of a screening protocol for Salmonella spp. and enteropathogenic Yersinia spp. in samples from wild boar (Sus scrofa) also generating MLVA-data for Y. enterocolitica and Y. pseudotuberculosis.

J Microbiol Methods 2018 Jul 21;150:32-38. Epub 2018 May 21.

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden; Department of Microbiology, National Veterinary Institute, Uppsala, Sweden. Electronic address:

Salmonellosis and yersiniosis are notifiable human diseases that are commonly associated with contaminated food. Domestic pigs as well as wild boars and other wild-life have been identified as reservoirs of these bacteria. Methods for cultivation and molecular epidemiological investigations of Salmonella spp. Read More

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July 2018
6 Reads

Effects of Psidium guajava leaf extract on secretion systems of Gram-negative enteropathogenic bacteria.

Microbiol Immunol 2018 May 23. Epub 2018 May 23.

Department of Bacteriology, Graduate School of Medicine, University of the Ryukyus, Nishihara, Okinawa 903- 0215, Japan.

We screened a total of 672 plant-tissue extracts to search for phytochemicals that inhibit the function of the type III secretion system (T3SS) of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). Among candidates examined, we found that an extract from the leaves of Psidium guajava (guava) inhibited the secretion of the EspB protein from EPEC and EHEC without affecting bacterial growth. Read More

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May 2018
2 Reads

Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against .

Bioinform Biol Insights 2018 14;12:1177932218755337. Epub 2018 May 14.

Institute of Technology and Research, Universidade Tiradentes, Aracaju, Brazil.

Caseous lymphadenitis (CLA) is a disease caused by bacteria that affects sheep and goats. The absence of a serologic diagnose is a factor that contributes for the disease dissemination, and due to the formation of granuloma, the treatment is very expensive. Therefore, prophylaxis is the approach with best cost-benefit relation; however, it still lacks an effective vaccine. Read More

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May 2018
3 Reads

YopN Is Required for Efficient Effector Translocation and Virulence in Yersinia pseudotuberculosis.

Infect Immun 2018 Aug 23;86(8). Epub 2018 Jul 23.

Department of Molecular Biology, Umeå Centre for Microbial Research UCMR, Umeå University, Umeå, Sweden

Type III secretion systems (T3SSs) are used by various Gram-negative pathogens to subvert the host defense by a host cell contact-dependent mechanism to secrete and translocate virulence effectors. While the effectors differ between pathogens and determine the pathogenic life style, the overall mechanism of secretion and translocation is conserved. T3SSs are regulated at multiple levels, and some secreted substrates have also been shown to function in regulation. Read More

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August 2018
4 Reads

Recombinant M. bovis BCG expressing the PLD protein promotes survival in mice challenged with a C. pseudotuberculosis virulent strain.

Vaccine 2018 06;36(25):3578-3583

Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.

The aim of this study was to evaluate the survival of mice inoculated with M. bovis BCG Pasteur recombinant expressing the PLD protein and challenged with a C. pseudotuberculosis virulent strain. Read More

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June 2018
2 Reads

Complete Genome Sequences of 10 Yersinia pseudotuberculosis Isolates Recovered from Wild Boars in Germany.

Genome Announc 2018 May 10;6(19). Epub 2018 May 10.

German Federal Institute for Risk Assessment, Berlin, Germany

We report here the draft genome sequences of 10 isolates recovered from tonsils of wild boars hunted between 2015 and 2016 in Germany. Whole-genome sequencing and bioinformatic analyses were performed to assess the diversity of , which may result in human infections caused by the consumption of game meat. Read More

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May 2018
2 Reads

Outbreaks of diarrhoea ('winter scours') in weaned Merino sheep in south-eastern Australia.

Aust Vet J 2018 May;96(5):176-183

Mackinnon Project, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.

Objective: Identify the cause of outbreaks of diarrhoea during winter that are not attributable to gastrointestinal nematodes in weaned Merino sheep in the high rainfall regions of south-eastern Australia and determine the efficacy of antimicrobials used to treat this syndrome.

Methods: We investigated 45 outbreaks on 24 farms. Faecal samples from affected animals were cultured for Yersinia, Campylobacter and Salmonella spp. Read More

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May 2018
5 Reads

Identification of a novel aminopolycarboxylic acid siderophore gene cluster encoding the biosynthesis of ethylenediaminesuccinic acid hydroxyarginine (EDHA).

Metallomics 2018 May;10(5):722-734

Interfaculty Institute of Microbiology and Infection Medicine Tuebingen, Microbiology/Biotechnology, University of Tuebingen, Tuebingen, Germany.

The mechanism of siderophore-mediated iron supply enhances fitness and survivability of microorganisms under iron limited growth conditions. One class of naturally occurring ionophores is the small aminopolycarboxylic acids (APCAs). Although they are structurally related to the most famous anthropogenic chelating agent, ethylenediaminetetraacetate (EDTA), they have been largely neglected by the scientific community. Read More

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May 2018
2 Reads

A longitudinal study of faecal shedding of Yersinia enterocolitica and Yersinia pseudotuberculosis by Merino lambs in south-eastern Australia.

Prev Vet Med 2018 May 1;153:30-41. Epub 2018 Mar 1.

Mackinnon Project, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, 250 Princes Highway, Werribee, VIC 3030, Australia.

A prospective longitudinal study was conducted to investigate potential risk factors for faecal shedding of Yersinia enterocolitica and Y. pseudotuberculosis by Merino lambs in four flocks in south-eastern Australia. The primary aims of the study were to determine the seasonal patterns of shedding of pathogenic Y. Read More

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May 2018
4 Reads

A Transcription Factor-Based Biosensor for Detection of Itaconic Acid.

ACS Synth Biol 2018 May 17;7(5):1436-1446. Epub 2018 Apr 17.

BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences , The University of Nottingham , Nottingham , NG7 2RD , United Kingdom.

Itaconic acid is an important platform chemical that can easily be incorporated into polymers and has the potential to replace petrochemical-based acrylic or methacrylic acid. A number of microorganisms have been developed for the biosynthesis of itaconate including Aspergillus terreus, Escherichia coli, and Saccharomyces cerevisiae. However, the number of strains and conditions that can be tested for increased itaconate titers are currently limited because of the lack of high-throughput screening methods. Read More

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May 2018
2 Reads