Wzx flippases exhibiting complex O-unit preferences require a new model for Wzx-substrate interactions.
Microbiologyopen 2018 Jun 10:e00655. Epub 2018 Jun 10.
School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales, Australia.
The Wzx flippase is a critical component of the O-antigen biosynthesis pathway, being responsible for the translocation of oligosaccharide O units across the inner membrane in Gram-negative bacteria. Recent studies have shown that Wzx has a strong preference for its cognate O unit, but the types of O-unit structural variance that a given Wzx can accommodate are poorly understood. In this study, we identified two Yersinia pseudotuberculosis Wzx that can distinguish between different terminal dideoxyhexose sugars on a common O-unit main-chain, despite both being able to translocate several other structurally-divergent O units. Read More