37,444 results match your criteria Pseudomonas Aeruginosa Infections


Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of .

Pharmaceutics 2020 Jul 6;12(7). Epub 2020 Jul 6.

Department of Biochemistry and Molecular Biology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.

Current treatments against bacterial infections have severe limitations, mainly due to the emergence of resistance to conventional antibiotics. In the specific case of strains, they have shown a number of resistance mechanisms to counter most antibiotics. Human secretory RNases from the RNase A superfamily are proteins involved in a wide variety of biological functions, including antimicrobial activity. Read More

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http://dx.doi.org/10.3390/pharmaceutics12070631DOI Listing

Frequency and antimicrobial susceptibility of bacteria causing bloodstream infections in pediatric patients from United States (US) medical centers (2014-2018): therapeutic options for multidrug-resistant bacteria.

Diagn Microbiol Infect Dis 2020 Jun 13;98(2):115108. Epub 2020 Jun 13.

JMI Laboratories, North Liberty, Iowa, USA.

Studies evaluating large series of pediatric patients with bloodstream infections (BSIs) are scarce. We evaluated the frequency and antimicrobial susceptibility of organisms isolated from pediatric patients with BSI and therapeutic options for BSI caused by multidrug-resistant (MDR) organisms. A total of 2423 organisms were consecutively collected from 33 US medical centers between 2014 and 2018, and susceptibility was tested by reference broth microdilution methods. Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2020.115108DOI Listing

Participation of the IL-10RB Related Cytokines, IL-22 and IFN-λ in Defense of the Airway Mucosal Barrier.

Front Cell Infect Microbiol 2020 19;10:300. Epub 2020 Jun 19.

Department of Pediatrics, Columbia University Medical Center, New York, NY, United States.

The airway epithelial barrier is a major barrier protecting against clinically significant infections of the lung. Its integrity is often compromised due to mechanical, chemical, or infectious causes. Opportunistic bacterial pathogens are poised to cause parenchymal infection and become difficult to eradicate due to adaptive metabolic changes, biofilm formation, and the acquisition of antimicrobial resistance and fitness genes. Read More

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http://dx.doi.org/10.3389/fcimb.2020.00300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318800PMC

Transcriptional regulation of microRNA in apple () and the heterologous plant system .

Hortic Res 2020 1;7:99. Epub 2020 Jul 1.

Department of Genomics and Biology of Fruit Crops, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38010 Italy.

microRNA MdmiR285N is a potential key regulator of plant immunity, as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in plant defense to pathogens. In this study, the promoter region of was isolated from the apple genome and analyzed in silico to detect potential regulatory regions controlling its transcription. A complex network of putative regulatory elements involved in plant growth and development, and in response to different hormones and stress conditions, was identified. Read More

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http://dx.doi.org/10.1038/s41438-020-0321-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326934PMC

Mechanisms for Rapid Evolution of Carbapenem Resistance in a Clinical Isolate of .

Front Microbiol 2020 19;11:1390. Epub 2020 Jun 19.

State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.

Infections by are difficult to cure due to its high intrinsic and acquired antibiotic resistance. Once colonized the human host, and thanks to antibiotic treatment pressure, usually acquires genetic mutations which provide bacteria with antibiotic resistance as well as ability to better adapt to the host environment. Deciphering the evolutionary traits may provide important insights into the development of effective combinatory antibiotic therapy to treat infections. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318546PMC

A Novel Cecropin D-Derived Short Cationic Antimicrobial Peptide Exhibits Antibacterial Activity Against Wild-Type and Multidrug-Resistant Strains of and .

Evol Bioinform Online 2020 26;16:1176934320936266. Epub 2020 Jun 26.

Valle del Cauca Departmental Secretary of Health, Gobernación del Valle del Cauca, Cali, Colombia.

Infections caused by multidrug-resistant (MDR) and are a serious worldwide public health concern due to the ineffectiveness of empirical antibiotic therapy. Therefore, research and the development of new antibiotic alternatives are urgently needed to control these bacteria. The use of cationic antimicrobial peptides (CAMPs) is a promising candidate alternative therapeutic strategy to antibiotics because they exhibit antibacterial activity against both antibiotic susceptible and MDR strains. Read More

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http://dx.doi.org/10.1177/1176934320936266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7323284PMC

The Nitrite Transporter Facilitates Biofilm Formation via Suppression of Nitrite Reductase and Is a New Antibiofilm Target in Pseudomonas aeruginosa.

mBio 2020 Jul 7;11(4). Epub 2020 Jul 7.

Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea

Biofilm-forming bacteria, including the Gram-negative , cause multiple types of chronic infections and are responsible for serious health burdens in humans, animals, and plants. Nitric oxide (NO) has been shown to induce biofilm dispersal via triggering a reduction in cyclic-di-GMP levels in a variety of bacteria. However, how NO, at homeostatic levels, also facilitates biofilm formation is unknown. Read More

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http://dx.doi.org/10.1128/mBio.00878-20DOI Listing

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-Resistant Pseudomonas aeruginosa.

Photodiagnosis Photodyn Ther 2020 Jul 4:101908. Epub 2020 Jul 4.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Brazil. Electronic address:

The interaction of metallic nanoparticles with light excites a local surface plasmon resonance (LSPR). This phenomenon enables the transfer of hot electrons to substrates that release Reactive Oxygen Species (ROS). In this context, the present study aimed at enhancing the antibacterial effect of citrate-covered silver nanoparticles (AgNPs) by LSPR excitation with visible LED. Read More

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http://dx.doi.org/10.1016/j.pdpdt.2020.101908DOI Listing

Amiodarone that has antibacterial effect against human pathogens may represent a novel catheter lock.

Acta Microbiol Immunol Hung 2020 Jul 6. Epub 2020 Jul 6.

1Department of Medical Microbiology, Medical School, University of Pecs, Pecs, Hungary.

Infection is one of the most feared hospital-acquired complications. Infusion therapy is frequently administered through a central line. Infusions facilitating bacterial growth may be a source of central line-associated bloodstream infections. Read More

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http://dx.doi.org/10.1556/030.2020.01144DOI Listing

The histone-like protein AlgP regulon is distinct in mucoid and nonmucoid and does not include alginate biosynthesis genes.

Microbiology 2020 Jul 7. Epub 2020 Jul 7.

Emory+Children's Center for Cystic Fibrosis and Airway Disease Research, Emory University School of Medicine, Atlanta, GA, USA.

The opportunistic bacterial pathogen causes acute and chronic infections that are notoriously difficult to treat. In people with cystic fibrosis, can cause lifelong lung infections, and isolation of mucoid , resulting from the overproduction of alginate, is associated with chronic infection. The histone-like protein AlgP has previously been implicated in the control of alginate gene expression in mucoid strains, but this regulation is unclear. Read More

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http://dx.doi.org/10.1099/mic.0.000923DOI Listing

Maintenance tobramycin primarily affects untargeted bacteria in the CF sputum microbiome.

Thorax 2020 Jul 6. Epub 2020 Jul 6.

Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA

Rationale: The most common antibiotic used to treat people with cystic fibrosis (PWCF) is inhaled tobramycin, administered as maintenance therapy for chronic lung infections. While the effects of inhaled tobramycin on abundance and lung function diminish with continued therapy, this maintenance treatment is known to improve long-term outcomes, underscoring how little is known about why antibiotics work in CF infections, what their effects are on complex CF sputum microbiomes and how to improve these treatments.

Objectives: To rigorously define the effect of maintenance tobramycin on CF sputum microbiome characteristics. Read More

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http://dx.doi.org/10.1136/thoraxjnl-2019-214187DOI Listing

-transgenic BALB/c mice as a model of infections of the cystic fibrosis lung.

Infect Immun 2020 Jul 6. Epub 2020 Jul 6.

Department of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, MD

The opportunistic pathogen is responsible for much of the morbidity and mortality associated with cystic fibrosis (CF), a condition that predisposes patients to chronic lung infections. lung infections are difficult to treat because adapts to the CF lung, can develop multidrug resistance, and can form biofilms. Despite the clinical significance of , modeling infections in CF has been challenging. Read More

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http://dx.doi.org/10.1128/IAI.00237-20DOI Listing

Short chain α-pyrones capable of potentiating penicillin G against Pseudomonas aeruginosa.

Bioorg Med Chem Lett 2020 Aug 2;30(16):127301. Epub 2020 Jun 2.

Department of Chemistry, University of North Carolina Asheville, One University Heights, Asheville, NC 28804, United States. Electronic address:

The dramatic increase in bacterial resistance over the past three decades has greatly reduced the effectiveness of nearly all clinical antibiotics, bringing infectious disease to the forefront as a dire threat to global health. To combat these infections, adjuvant therapies have emerged as a way to reactivate known antibiotics against resistant pathogens. Herein, we report the evaluation of simplified α-pyrone adjuvants capable of potentiating penicillin G against Pseudomonas aeruginosa, a Gram-negative pathogen whose multidrug-resistant strains have been labeled by the Centers for Disease Control and Prevention as a serious threat to public health. Read More

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http://dx.doi.org/10.1016/j.bmcl.2020.127301DOI Listing

Phenotypic synergy testing of ceftazidime-avibactam with aztreonam in a university hospital having high number of metallobetalactamase producing bacteria.

Infect Dis (Lond) 2020 Jul 6:1-7. Epub 2020 Jul 6.

Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.

Ceftazidime-avibactam combination with aztreonam and role of rapid synergy reporting has not been widely evaluated. Also the synergy correlation with various betalactamases has not been widely studied. We studied phenotypic synergy testings and molecular detection of betalactamases in our university hospital where we have large number of mellatobetalactmase producing bacteria. Read More

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http://dx.doi.org/10.1080/23744235.2020.1788224DOI Listing

A rapid synthesis of low-nanomolar divalent LecA inhibitors in four linear steps from d-galactose pentaacetate.

Chem Commun (Camb) 2020 Jul 6. Epub 2020 Jul 6.

Chemical Biology of Carbohydrates, Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Centre for Infection Research, 66123 Saarbrücken, Germany. and Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124 Braunschweig, Germany and Department of Pharmacy, Saarland University, 66123 Saarbrücken, Germany.

Chronic infections with Pseudomonas aeruginosa are associated with the formation of bacterial biofilms. The tetrameric P. aeruginosa lectin LecA is a virulence factor and an anti-biofilm drug target. Read More

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http://dx.doi.org/10.1039/d0cc03490hDOI Listing

Methods for Extraction and Detection of Pf Bacteriophage DNA from the Sputum of Patients with Cystic Fibrosis.

Phage 2020 Jun 16;1(2):100-108. Epub 2020 Jun 16.

Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, California, USA.

There is increasing interest in the pulmonary microbiome's bacterial and viral communities, particularly in the context of chronic airway infections in cystic fibrosis (CF). However, the isolation of microbial DNA from the sputum from patients with CF is technically challenging and the optimal protocols for the analysis of viral species, including bacteriophage, from clinical samples remains difficult. In this study, we evaluate a set of methods developed for processing and analyzing sputum from patients with CF with the goal of detecting Pf bacteriophage virion-derived nucleic acid. Read More

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http://dx.doi.org/10.1089/phage.2020.0003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327540PMC

Airway clearance techniques in bronchiectasis: Analysis from the United States Bronchiectasis and NTM Research Registry.

Chest 2020 Jul 2. Epub 2020 Jul 2.

Department of Pulmonology, OHSU School of Medicine, Portland, OR; Department of Infectious Disease, OHSU School of Medicine, Portland, OR.

Background: In patients with bronchiectasis, airway clearance techniques (ACTs) are important management strategies.

Research Question: The primary objective was to describe differences in patients with bronchiectasis and productive cough who utilized ACTs and those who did not. Secondary objectives included assessment of bronchiectasis exacerbation frequency and change in pulmonary function at one-year follow-up. Read More

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http://dx.doi.org/10.1016/j.chest.2020.06.050DOI Listing

The seventh nationwide surveillance of six otorhinolaryngological infectious diseases and the antimicrobial susceptibility patterns of the isolated pathogens in Japan.

J Infect Chemother 2020 Jul 1. Epub 2020 Jul 1.

Otorhinolaryngological Sub-committee and the Surveillance Committee of Japanese Society of Chemotherapy (JSC), The Japanese Association for Infectious Diseases (JAID), The Japanese Society for Clinical Microbiology (JSCM), Tokyo, Japan.

The Japanese Three Academic Societies Joint Antimicrobial Susceptibility Surveillance Committee conducted a nationwide surveillance on six otorhinolaryngological diseases and investigated the antimicrobial susceptibility patterns and isolation rates of the causative pathogens. The surveillance program was conducted in the otorhinolaryngological departments of 12 universities, and 36 affiliated hospitals and clinics. Patients with acute otitis media (children under six years old), chronic otitis media, acute nasal sinusitis, chronic nasal sinusitis, acute tonsillitis, and peritonsillar abscess (over 20 years old) between December 2015 and June 2017 were investigated. Read More

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http://dx.doi.org/10.1016/j.jiac.2020.05.020DOI Listing

Activity of ceftolozane-tazobactam and comparators when tested against bacterial surveillance isolates collected from patients at risk of infections caused by resistant gram-negative pathogens.

Diagn Microbiol Infect Dis 2020 Jun 6;98(1):115101. Epub 2020 Jun 6.

JMI Laboratories, North Liberty, Iowa, USA.

Ceftolozane-tazobactam is an antipseudomonal cephalosporin combined with a β-lactamase inhibitor. Ceftolozane-tazobactam has been approved for treatment of complicated urinary tract infections and acute pyelonephritis, for complicated intra-abdominal infections (with metronidazole) in adults, and for hospital-acquired bacterial pneumonia including ventilator-associated bacterial pneumonia. This study analyzed gram-negative pathogen susceptibility in US and European patients who are considered at risk for infections caused by pathogens resistant to commonly used antimicrobials: patients in the intensive care unit (ICU), patients on the hematology/oncology or transplant service who may be immunocompromised, and patients >65 years old (yo). Read More

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http://dx.doi.org/10.1016/j.diagmicrobio.2020.115101DOI Listing

Moving away from traditional antibiotic treatment: can macrocyclic lactones from marine macroalga-associated heterotroph be the alternatives?

Appl Microbiol Biotechnol 2020 Jul 3. Epub 2020 Jul 3.

Crustacean Fisheries Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.

Intertidal red algae Hypnea valentiae associated Bacillus amyloliquefaciens MTCC 12716 revealed potential inhibitory effects on the growth of drug-resistant pathogens. In the genome of B. amyloliquefaciens MTCC 12716, biosynthetic gene clusters encoding antibacterial metabolites were predicted, which might be expressed and contributed to the broad-spectrum anti-infective activity. Read More

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http://dx.doi.org/10.1007/s00253-020-10658-0DOI Listing

Efficacy of experimental phage therapies in livestock.

Anim Health Res Rev 2020 Jun 19:1-15. Epub 2020 Jun 19.

Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Basis of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland.

Bacteriophages are the most abundant form of life on earth and are present everywhere. The total number of bacteriophages has been estimated to be 1032 virions. The main division of bacteriophages is based on the type of nucleic acid (DNA or RNA) and on the structure of the capsid. Read More

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http://dx.doi.org/10.1017/S1466252319000161DOI Listing

The combined antimicrobial activity of citrus honey and fosfomycin on multidrug resistant isolates.

AIMS Microbiol 2020 19;6(2):162-175. Epub 2020 Jun 19.

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Infections with () have become a real fear in hospital-acquired infections, especially in critically ill and immunocompromised patients. Thus, advance of novel anti-infectives is currently pursued. The aim of the present study was to evaluate the antibacterial effect of each of citrus honey and fosfomycin in comparison to the combined effect of both of them on multidrug resistant (MDR) . Read More

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http://dx.doi.org/10.3934/microbiol.2020011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326728PMC

Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival.

Sci Rep 2020 Jul 2;10(1):10935. Epub 2020 Jul 2.

Department of Medicine, Dartmouth-Hitchcock Medical Center, Geisel School of Medicine at Dartmouth, 1 Medical Center Dr, Lebanon, NH, 03756, USA.

Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, resulting in chronic bacterial lung infections and tissue damage. CF macrophages exhibit reduced bacterial killing and increased inflammatory signaling. Iron is elevated in the CF lung and is a critical nutrient for bacteria, including the common CF pathogen Pseudomonas aeruginosa (Pa). Read More

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http://dx.doi.org/10.1038/s41598-020-67729-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331733PMC

Emergence of multidrug-resistant non-fermentative gram negative bacterial infection in hospitalized patients in a tertiary care center of Nepal.

BMC Res Notes 2020 Jul 2;13(1):319. Epub 2020 Jul 2.

Department of Clinical Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.

Objective: This study was designed for the characterization and establishment of antibiotic susceptibility profiles of non-fermentative gram negative bacteria isolated from hospitalized patients in a tertiary care hospital of Nepal.

Results: A total of 402 non-fermentative gram negative bacteria was isolated in 1486 culture-positive cases from 6216 different clinical samples obtained from hospitalized patients. Among total non-fermentative gram negative bacterial isolates, the highest number was recovered from specimens collected from lower respiratory tract infections (n = 173, 43. Read More

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http://dx.doi.org/10.1186/s13104-020-05163-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330544PMC

Biofilm modelling on the contact lenses and comparison of the in vitro activities of multipurpose lens solutions and antibiotics.

PeerJ 2020 24;8:e9419. Epub 2020 Jun 24.

Department of Pharmaceutical Microbiology, Istanbul University, Faculty of Pharmacy, Istanbul, Turkey.

During the contact lens (CL) usage, microbial adhesion and biofilm formation are crucial threats for eye health due to the development of mature biofilms on CL surfaces associated with serious eye infections such as keratitis. For CL related eye infections, multi drug resistant or (especially MRSA) and are the most common infectious bacteria and yeast, respectively. In this study, CL biofilm models were created by comparing them to reveal the differences on specific conditions. Read More

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http://dx.doi.org/10.7717/peerj.9419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320721PMC

Bacterial associated urinary tract infection, risk factors, and drug susceptibility profile among adult people living with HIV at Haswassa University Comprehensive Specialized Hospital, Hawassa, Southern Esthiopia.

Sci Rep 2020 Jul 1;10(1):10790. Epub 2020 Jul 1.

School of Medical Laboratory Science, Hawassa University College of Medicine and Health Sciences, Awassa, Ethiopia.

People living with human immunodeficiency virus (HIV) are more likely to develop urinary tract infections (UTI) due to the suppression of their immunity. The aim of this study was to determine the prevalence, risk factors of UTI, and drug susceptibility pattern of bacteria isolated among peoples infected with HIV. A hospital-based cross-sectional study was conducted among 224 HIV positive individuals attending Hawassa University Comprehensive Specialized Hospital (HUCSH) from September 17 to November 16, 2018. Read More

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http://dx.doi.org/10.1038/s41598-020-67840-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330024PMC

Moxifloxacin Loaded Lipidic Nanoparticles for Antimicrobial Efficacy.

Curr Pharm Des 2020 Jul 1. Epub 2020 Jul 1.

Department of Pharmaceutics, School of Pharmacy, Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran. Iran.

Background: Pulmonary infections are an increasing problem in individuals and current therapies are lacking. Liposomes are spherical lipidic vesicles composed of phospholipid and cholesterol. Liposomes have numerous advantages, such as biodegradability, biocompatibility, non-immunogenicity, lack of toxicity, controlled release properties and high stability. Read More

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http://dx.doi.org/10.2174/1381612826666200701152618DOI Listing

Characterization of Bacteriophages against pv. with Potential Use as Natural Antimicrobials in Kiwifruit Plants.

Microorganisms 2020 Jun 29;8(7). Epub 2020 Jun 29.

Laboratory of Microbiology, Institute of Biology, Pontificia Universidad Católica de Valparaíso, Av. Universidad # 330, Curauma, 2340000 Valparaíso, Chile.

pv. (Psa) is the causal agent of a bacterial canker in kiwifruit plants and has caused economic losses worldwide. Currently, the primary strategies to control this pathogen include the use of copper-based compounds and even antibiotics. Read More

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http://dx.doi.org/10.3390/microorganisms8070974DOI Listing

Using minimum inhibitory concentration values of common topical antibiotics to investigate emerging antibiotic resistance: A retrospective study of 134 dogs and 20 horses with ulcerative keratitis.

Vet Ophthalmol 2020 Jul 1. Epub 2020 Jul 1.

College of Optometry, The Ohio State University, Columbus, OH, USA.

Objectives: To identify the minimum inhibitory concentration (MIC) distribution for commonly used topical antibiotics from isolates of dogs and horses with ulcerative bacterial keratitis, and to investigate changes in MIC values over time and following treatment with topical fluoroquinolones.

Animals Studied: One hundred thirty-four client-owned dogs and 20 client-owned horses with bacterial ulcerative keratitis.

Procedure: Minimum inhibitory concentration values for 14 topical antibiotics were reported for canine and equine cases of bacterial ulcerative keratitis between 2013 and 2018. Read More

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http://dx.doi.org/10.1111/vop.12801DOI Listing

Recent advances in tackling microbial multidrug resistance with essential oils: combinatorial and nano-based strategies.

Crit Rev Microbiol 2020 Jul 1:1-20. Epub 2020 Jul 1.

Department of Pharmacognosy, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Iasi, Romania.

The emergence of multidrug resistant pathogens is a great challenge to the medical field and a major global health threat requiring novel therapeutic strategies. Among plant products, essential oils have significant antimicrobial properties that make them promising agents in the fight against drug resistant human pathogens. The aim of the present review was to highlight the most important essential oil-based antimicrobial strategies as revealed by recent studies. Read More

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http://dx.doi.org/10.1080/1040841X.2020.1782339DOI Listing

Minimum Bactericidal Concentration of Ciprofloxacin to Determined Rapidly Based on Pyocyanin Secretion.

Sens Actuators B Chem 2020 Jun 4;312. Epub 2020 Mar 4.

Electrical and Computer Engineering, University of Virginia, Charlottesville, Virginia 22904, USA.

Infections due to () often exhibit broad-spectrum resistance and persistence to common antibiotics. Persistence is especially problematic with immune-compromised subjects who are unable to eliminate the inhibited bacteria. Hence, antibiotics must be used at the appropriate minimum bactericidal concentration (MBC) rather than at minimum inhibitory concentration (MIC) levels. Read More

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http://dx.doi.org/10.1016/j.snb.2020.127936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326315PMC

Bacterial and fungal coinfection among hospitalised patients with COVID-19: A retrospective cohort study in a UK secondary care setting.

Clin Microbiol Infect 2020 Jun 27. Epub 2020 Jun 27.

Chelsea and Westminster NHS Foundation Trust, 369 Fulham Road, London, SW10 9NH. UK; North West London Pathology, Imperial College Healthcare NHS Trust, Fulham Palace Road. London. W6 8RF. UK; National Institute for Health Research Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Imperial College London, Hammersmith Campus, Du Cane Road, London. W12 0NN. UK.

Objectives: We investigate the incidence of bacterial and fungal co-infection of hospitalised patients with confirmed SARS-CoV-2 in this retrospective observational study across two London hospitals during the first UK wave of COVID-19.

Methods: A retrospective case-series of hospitalised patients with confirmed SARS-CoV-2 by PCR was analysed across two acute NHS hospitals (February 20-April 20; each isolate reviewed independently in parallel). This was contrasted to a control group of influenza positive patients admitted during 2019/20 flu season. Read More

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http://dx.doi.org/10.1016/j.cmi.2020.06.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320692PMC

Evaluation of peptide-based probes towards diagnostic imaging of bacterial biofilm-associated infections.

ACS Infect Dis 2020 Jun 30. Epub 2020 Jun 30.

The clinical management of bacterial biofilm infections represents an enormous challenge in today's healthcare setting. The NIH estimates that 65% of bacterial infections are biofilm related and therapeutic outcomes are positively correlated with early intervention. Currently, there is no reliable imaging technique to detect biofilm infections and current clinical protocols for accurate and direct biofilm identification are non-existent. Read More

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http://dx.doi.org/10.1021/acsinfecdis.0c00125DOI Listing

In-Silico Analysis and Protective Efficacy of the PcrV Recombinant Vaccine against Pseudomonas Aeruginosa in the Burned and PA-Infected BALB/c Mouse Model.

Iran J Immunol 2020 Jun;17(2):121-136

Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.

Background: Pseudomonas aeruginosa is considered as the most severe cause of infections in burn patients and pneumonia infections.

Objective: To study the protective effects of recombinant protein vaccine harboring the PcrV of P. aeruginosa in the mouse model of burn and respiratory infections. Read More

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http://dx.doi.org/10.22034/iji.2020.85590.1718DOI Listing

A Structure-Function-Inhibition Analysis of the Type III Secretion Needle Protein PscF.

J Bacteriol 2020 Jun 29. Epub 2020 Jun 29.

Microbiotix, Inc., Worcester, MA, USA 01605.

The type III secretion system (T3SS) needle comprised of multiple PscF subunits is essential for the translocation of effector toxins into human cells, facilitating the establishment and dissemination of infection. Mutations in the gene provide resistance to the phenoxyacetamide (PhA) series of T3SS inhibitory chemical probes. To better understand PscF functions and interactions with PhA, alleles of with 71 single mutations altering 49 of the 85 residues of the encoded protein were evaluated for their effects on T3SS phenotypes. Read More

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http://dx.doi.org/10.1128/JB.00055-20DOI Listing

Update of the treatment of nosocomial pneumonia in the ICU.

Crit Care 2020 Jun 29;24(1):383. Epub 2020 Jun 29.

ICU, Trinity Centre for Health Science HRB-Wellcome Trust, St James's Hospital, Dublin, Ireland.

In accordance with the recommendations of, amongst others, the Surviving Sepsis Campaign and the recently published European treatment guidelines for hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP), in the event of a patient with such infections, empirical antibiotic treatment must be appropriate and administered as early as possible. The aim of this manuscript is to update treatment protocols by reviewing recently published studies on the treatment of nosocomial pneumonia in the critically ill patients that require invasive respiratory support and patients with HAP from hospital wards that require invasive mechanical ventilation. An interdisciplinary group of experts, comprising specialists in anaesthesia and resuscitation and in intensive care medicine, updated the epidemiology and antimicrobial resistance and established clinical management priorities based on patients' risk factors. Read More

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http://dx.doi.org/10.1186/s13054-020-03091-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322703PMC

Evaluation of the Etest and disk diffusion method for detection of the activity of ceftazidime-avibactam against Enterobacterales and Pseudomonas aeruginosa in China.

BMC Microbiol 2020 Jun 29;20(1):187. Epub 2020 Jun 29.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, 100044, China.

Background: Ceftazidime-avibactam was approved in China in 2019 for treating complicated intra-abdominal infections, hospital-acquired pneumonia, ventilator-associated pneumonia, and infections caused by Enterobacterales and Pseudomonas aeruginosa for which treatment options are limited. However, no currently available commercial systems have been approved for antimicrobial susceptibility testing of ceftazidime-avibactam in China. Here, we evaluated the Etest and disk diffusion method for detecting the activity of ceftazidime-avibactam against Enterobacterales and P. Read More

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http://dx.doi.org/10.1186/s12866-020-01870-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325266PMC

Bacterial Profile, Antimicrobial Susceptibility Pattern, and Associated Risk Factors Among Patients With Wound Infections at Debre Markos Referral Hospital, Northwest, Ethiopia.

Int J Low Extrem Wounds 2020 Jun 28:1534734620933731. Epub 2020 Jun 28.

University of Gondar, Gondar, Ethiopia.

Wound infections are associated with morbidity and mortality in developing countries. Thus, this study aimed to assess bacterial profile, antimicrobial susceptibility pattern and risk factors among wound infection suspected patients. A hospital-based cross-sectional study was conducted on 201 participants at Debre Markos referral hospital from January to May 2019. Read More

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http://dx.doi.org/10.1177/1534734620933731DOI Listing

Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds.

J Microbiol Methods 2020 Jun 25;175:105994. Epub 2020 Jun 25.

Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, Turku 20520, Finland. Electronic address:

Polymicrobial biofilms are major complications of various chronic infections. Therefore, in vitro biorelevant polymicrobial biofilm models are essential tools for medical studies. This study presents an in vitro model for dual species biofilm of Pseudomonas aeruginosa and Staphylococcus aureus developed on cell-derived matrices (CDMs), in order to simulate the microenvironment of in vivo biofilms. Read More

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http://dx.doi.org/10.1016/j.mimet.2020.105994DOI Listing

Healthcare-associated infections in burn patients: Timeline and risk factors.

Burns 2020 May 16. Epub 2020 May 16.

Burn Unit, Hospital Universitario del Valle, Universidad del Valle, Cali, Colombia.

Background: Healthcare-associated infections (HAIs) remain a major challenge in burn research and care. We aimed to describe the epidemiology and timeline of HAIs and to estimate the association of demographics and clinical characteristics with time to HAI among burn patients.

Methods: A prospective cohort study was conducted in a referral burn unit in southwestern Colombia. Read More

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http://dx.doi.org/10.1016/j.burns.2020.04.031DOI Listing

Pre-operative methicillin resistant Staphylococcus aureus results do not predict surgical site infections in children undergoing varus derotational osteotomy.

Medicine (Baltimore) 2020 Jun;99(26):e20517

Keck School of Medicine, University of Southern California.

Literature regarding the value of pre-operative nasal methicillin resistant Staphylococcus aureus (MRSA) swabs to predict surgical site infections (SSIs) in children undergoing lower extremity surgery is limited. The purpose of our study was to determine if pre-operative nasal MRSA swab results were predictive of SSI development in children undergoing a femoral varus derotational osteotomy (VDRO).Patients who underwent VDRO between 2004-2016 were reviewed to determine pre-operative MRSA colonization rates and SSI devolvement rates. Read More

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http://dx.doi.org/10.1097/MD.0000000000020517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329002PMC
June 2020
5.723 Impact Factor

Multidrug-Resistant Organisms from Ophthalmic Cultures: Antibiotic Resistance and Visual Acuity.

Mil Med 2020 Jun 26. Epub 2020 Jun 26.

Department of Ophthalmology, Brooke Army Medical Center, 3551 Roger Brooke Drive, San Antonio, TX 78234.

Introduction: There is a growing trend of multidrug-resistant organisms (MDRO). The goal of this study was to characterize MDRO at a single center from ophthalmic cultures to better understand how treatments were tailored and to assess effect on visual acuity.

Materials And Methods: The MDRO data were collected by the Multidrug-Resistant Organism Repository and Surveillance Network from the Brooke Army Medical Center clinical laboratory. Read More

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http://dx.doi.org/10.1093/milmed/usaa111DOI Listing

Epidemiology, prevalence and risk factors for infections in burn patients: results from a regional burn centre's analysis.

J Chemother 2020 Jun 26:1-5. Epub 2020 Jun 26.

Department of Medical Sciences, Infectious Diseases, University of Turin, Turin, Italy.

Burn patients are at high risk of infections due to severe impairment of immunity and loss of skin barrier function. We aimed to describe the epidemiology, incidence and risk factors for infection in a cohort of burns patients. Two hundred patients were retrospectively enrolled and subdivided into infected (N = 81) and uninfected groups (N = 119). Read More

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http://dx.doi.org/10.1080/1120009X.2020.1780776DOI Listing

Molecular tracking of pathogens in central venous catheter.

J Vasc Access 2020 Jun 26:1129729820934354. Epub 2020 Jun 26.

Department of Social and Preventive Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

Background: Central venous catheter-related bloodstream infection is an important adverse event in health care. Molecular methods are not yet substitutive of microbiological in the detection of the pathogens responsible for the infection, but they can help in the epidemiological characterization.

Aim: To detect bacteria by polymerase chain reaction, from material extracted from the tip of central catheters of patients suspected of infection at the intensive care unit. Read More

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http://dx.doi.org/10.1177/1129729820934354DOI Listing

Periodontal condition and periodontal risk assessment in adult patients with cystic fibrosis.

Ann Agric Environ Med 2020 Jun 11;27(2):235-239. Epub 2019 Apr 11.

Department of Paediatric Dentistry, University of Medical Sciences, Poznań, Poland.

Introduction: The presence of chronic inflammation in the mouth, such as infectious disease of the periodontal tissues, may be the reservoir of microorganisms that are not usually present, including Pseudomonas aeruginosa.

Objective: The purpose of the study was to create a profile of periodontal conditions and periodontal risk assessment in adult patients with cystic fibrosis.

Material And Methods: The study involved 22 patients with cystic fibrosis (CF) aged 29. Read More

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http://dx.doi.org/10.26444/aaem/106087DOI Listing

Complete Genome Sequence of Pseudomonas aeruginosa Strain AA2 (LMG 27630), an Early Isolate Recovered from the Airway of a German Cystic Fibrosis Patient.

Microbiol Resour Announc 2020 Jun 25;9(26). Epub 2020 Jun 25.

Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium

is an opportunistic pathogen that is able to cause various infections, including airway infections in cystic fibrosis patients. Here, we present the complete closed and annotated genome sequence of AA2, an isolate obtained early during infection of the respiratory tract of a German cystic fibrosis patient. Read More

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http://dx.doi.org/10.1128/MRA.00526-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317106PMC

Combinatorial effects of antibiotics and enzymes against dual-species Staphylococcus aureus and Pseudomonas aeruginosa biofilms in the wound-like medium.

PLoS One 2020 25;15(6):e0235093. Epub 2020 Jun 25.

Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Bacterial biofilms are one of the major issues in the treatment of chronic infections such as chronic wounds, where biofilms are typically polymicrobial. The synergy between species can occur during most polymicrobial infections, where antimicrobial resistance enhances as a result. Furthermore, self-produced extracellular polymeric substance (EPS) in biofilms results in a high tolerance to antibiotics that complicates wound healing. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235093PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316268PMC

Two Forms of Tyrosyl-tRNA Synthetase from : Characterization and Discovery of Inhibitory Compounds.

SLAS Discov 2020 Jun 25:2472555220934793. Epub 2020 Jun 25.

The University of Texas-RGV, Edinburg, TX, USA.

is a multidrug-resistant (MDR) pathogen and a causative agent of both nosocomial and community-acquired infections. The genes (S and Z) encoding both forms of tyrosyl-tRNA synthetase (TyrRS-S and TyrRS-Z) were cloned and the resulting proteins purified. TyrRS-S and TyrRS-Z were kinetically evaluated and the values for interaction with Tyr, ATP, and tRNA were 172, 204, and 1. Read More

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http://dx.doi.org/10.1177/2472555220934793DOI Listing

Occurrence and Predictors of Bacterial Respiratory Tract Infections and Antimicrobial Resistance Among Isolates From Dogs Presented With Lower Respiratory Tract Infections at a Referral Veterinary Hospital in South Africa.

Front Vet Sci 2020 2;7:304. Epub 2020 Jun 2.

Section Veterinary Public Health, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.

Respiratory tract infections (RTIs) associated with , Streptococci, Staphylococci, and species have been reported in dogs. The objective of this study was to investigate the occurrence and predictors of bacterial RTIs and antimicrobial resistance among samples from dogs with lower RTIs at a referral veterinary teaching hospital in South Africa. Records of 157 dogs with lower RTIs presented to the veterinary teaching hospital between 2007 and 2013 were included in the study. Read More

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http://dx.doi.org/10.3389/fvets.2020.00304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280450PMC

Association of Comorbid Conditions with Six-month Survival and Disease Outcome in Patients of Necroinflammatory Otitis Externa.

J Coll Physicians Surg Pak 2020 May;30(5):498-502

Department of Pathology, King Edward Medical University, Lahore, Pakistan.

Objective: To determine association of gender, causative organisms, control of diabetes, facial paralysis, infectious agent, and hearing loss with disease outcome, in terms of six-month improvement of symptoms, static condition or expiry of patients presenting with necroinflammatory otitis externa (NOE).

Study Design: Descriptive study.

Place And Duration Of Study: ENT Department in collaboration with Pathology Department, KEMU/Mayo Hospital, Lahore from 2016 to 2019. Read More

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http://dx.doi.org/10.29271/jcpsp.2020.05.498DOI Listing
May 2020
0.318 Impact Factor