34,772 results match your criteria Pseudomonas Aeruginosa Infections


Hydrogen peroxide-inactivated bacteria induces potent humoral and cellular immune responses and releases nucleic acids.

Int Immunopharmacol 2019 Feb 16;69:389-397. Epub 2019 Feb 16.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu 610041, People's Republic of China. Electronic address:

The problem of nosocomial infection is seriously escalating. Bacterial vaccines are indispensable for preventing infections caused by multi-drug resistant organisms. Some researchers have put forward the use of hydrogen peroxide (HO) as a new technology platform for virus deactivation. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.01.055DOI Listing
February 2019

Development of a Novel Loop-Mediated Isothermal Amplification Method to Detect Guiana Extended-Spectrum (GES) β-Lactamase Genes in .

Front Microbiol 2019 4;10:25. Epub 2019 Feb 4.

Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.

Infections caused by multidrug-resistant in hospitalized patients are often fatal, and nosocomial infections caused by Guiana extended-spectrum (GES) β-lactamase-producing strains are of growing concern. Several genotypes of the GES β-lactamase gene () include a single missense mutation, a change from G to A at nucleotide position 493 (G493A) that changes glycine to serine; the mutant enzyme exhibits carbapenemase activity. Rapid and reliable identification of drug-resistance is important in clinical settings; however, culture methods remain the gold standard. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369207PMC
February 2019

Help, hinder, hide and harm: what can we learn from the interactions between and during respiratory infections?

Thorax 2019 Feb 18. Epub 2019 Feb 18.

Departments of Pediatrics and Microbiology, University of Washington, Seattle, Washington, USA.

Recent studies of human respiratory secretions using culture-independent techniques have found a surprisingly diverse array of microbes. Interactions among these community members can profoundly impact microbial survival, persistence and antibiotic susceptibility and, consequently, disease progression. Studies of polymicrobial interactions in the human microbiota have shown that the taxonomic and structural compositions, and resulting behaviours, of microbial communities differ substantially from those of the individual constituent species and in ways of clinical importance. Read More

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http://dx.doi.org/10.1136/thoraxjnl-2018-212616DOI Listing
February 2019

Diagnosis and successful management of a rare case of phaeohyphomycosis in a Doberman pinscher dog.

Iran J Vet Res 2018 ;19(4):321-324

Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh-281001, India.

Skin affections in canines are common and their treatment depends upon the pathogens involved. Delayed or no response to traditional treatment indicates the involvement of unusual etiology or the multidrug resistance. This report describes the diagnosis and therapeutic management of a rare case of phaeohyphomycosis caused by complicated with associated pyoderma in a Doberman pinscher dog. Read More

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January 2018

Phenazine production promotes antibiotic tolerance and metabolic heterogeneity in Pseudomonas aeruginosa biofilms.

Nat Commun 2019 Feb 15;10(1):762. Epub 2019 Feb 15.

Department of Biological Sciences, Columbia University, New York, NY, 10027, USA.

Antibiotic efficacy can be antagonized by bioactive metabolites and other drugs present at infection sites. Pseudomonas aeruginosa, a common cause of biofilm-based infections, releases metabolites called phenazines that accept electrons to support cellular redox balancing. Here, we find that phenazines promote tolerance to clinically relevant antibiotics, such as ciprofloxacin, in P. Read More

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http://dx.doi.org/10.1038/s41467-019-08733-wDOI Listing
February 2019

Activity of ceftolozane-tazobactam against Pseudomonas aeruginosa and Enterobacterales isolates recovered in Intensive Care Units in Spain: The SUPERIOR multicentre study.

Int J Antimicrob Agents 2019 Feb 12. Epub 2019 Feb 12.

Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain; Red Española de Investigación en Patología Infecciosa (REIPI), Madrid, Spain. Electronic address:

Patients in intensive care units (ICUs) present a high risk of developing an infection caused by multi-drug resistant (MDR) bacteria. Consequently, new antimicrobials and combinations are needed. We evaluated the activity of ceftolozane-tazobactam against Enterobacterales (n=400) and Pseudomonas aeruginosa (n=80) isolates collected from patients in Spanish intensive care units (ICUs) with complicated urinary tract infections (cUTI) and complicated intra-abdominal infections (cIAI). Read More

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http://dx.doi.org/10.1016/j.ijantimicag.2019.02.004DOI Listing
February 2019
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Risk Assessment After a Severe Hospital-Acquired Infection Associated With Carbapenemase-Producing Pseudomonas aeruginosa.

JAMA Netw Open 2019 Feb 1;2(2):e187665. Epub 2019 Feb 1.

Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.

Importance: Resistance of gram-negative bacilli to carbapenems is rapidly emerging worldwide. In 2016, the World Health Organization defined the hospital-built environment as a core component of infection prevention and control programs. The hospital-built environment has recently been reported as a source for outbreaks and sporadic transmission events of carbapenemase-producing gram-negative bacilli from the environment to patients. Read More

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http://dx.doi.org/10.1001/jamanetworkopen.2018.7665DOI Listing
February 2019

Antibiotic resistance: Microbiological profile of urinary tract infections in Mexico.

Cir Cir 2019 ;87(2):176-182

Departamento de Inmunología, Centro de Investigación Biomédica de Occidente. Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México.

Objective: The objective of this study was to describe the prevalence, microbiological profile, bacterial resistance, and the sensitivity to antibiotics of microorganisms causing urinary tract infection (UTI) at a single-site tertiary referral hospital in the western region of Mexico.

Methods: A total of 5895 culture samples processed at the microbiology laboratory from August 1, 2014, to July 31, 2015, were analyzed.

Results: A total of 5895 samples for urine cultures (UC) were collected, of which 3363 were taken in women (57. Read More

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http://dx.doi.org/10.24875/CIRU.18000494DOI Listing
January 2019
1 Read

Electroceutical Treatment of Pseudomonas aeruginosa Biofilms.

Sci Rep 2019 Feb 14;9(1):2008. Epub 2019 Feb 14.

Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, 43210, USA.

Electroceutical wound dressings, especially those involving current flow with silver based electrodes, show promise for treating biofilm infections. However, their mechanism of action is poorly understood. We have developed an in vitro agar based model using a bioluminescent strain of Pseudomonas aeruginosa to measure loss of activity and killing when direct current was applied. Read More

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http://dx.doi.org/10.1038/s41598-018-37891-yDOI Listing
February 2019
2 Reads
5.078 Impact Factor

Monitoring the antimicrobial susceptibility of Gram-negative organisms involved in intraabdominal and urinary tract infections recovered during the SMART study (Spain, 2016 and 2017).

Rev Esp Quimioter 2019 Feb 13. Epub 2019 Feb 13.

Rafael Cantón. Servicio de Microbiología. Hospital Universitario Ramón y Cajal. Carretera de Colmenar Km 9,1. 28034 Madrid. Spain.

Objective: Continuous antimicrobial resistance surveillance is recommended by Public Health authorities. We up-dated data from the SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study in Spain.

Methods: The antimicrobial susceptibility data and extended-spectrum beta-lactamase (ESBL) production in isolates recovered from intra-abdominal (IAI) (n=1,429) and urinary tract (UTI) (n=937) infections during the 2016- 2017 SMART study in 10 Spanish hospitals were analysed. Read More

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February 2019

Cystic fibrosis - an example of personalized and precision medicine.

APMIS 2019 Feb 14. Epub 2019 Feb 14.

Cystic Fibrosis Center, Rigshospitalet, Copenhagen, Denmark.

Cystic fibrosis (CF) is a severe, monogenic, autosomal recessive disease caused by mutations in the CFTR (cystic fibrosis transmembrane regulator) gene, where disturbed chloride and bicarbonate transportation in epithelial cells results in a multiorgan disease with primarily pulmonary infections and pancreatic insufficiency. In 1968, the Copenhagen CF Center was established, and centralized care of CF patients with monthly control was introduced. Close monitoring and treatment of Pseudomonas lung infection as well as segregation of patients with different infection status improved the clinical outcome as well as survival. Read More

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http://dx.doi.org/10.1111/apm.12915DOI Listing
February 2019

Intra-Vesical Colistin for Urinary Tract Infections.

Eur J Case Rep Intern Med 2019 21;6(1):000996. Epub 2019 Jan 21.

Intensive Care Unit, Centro Hospitalar do Médio Tejo, Abrantes, Portugal.

The numbers of multiple drug-resistant Gram-negative bacterial infections are rising worldwide. The threat is increased by the lack of new antibiotics, so clinicians are turning to older drugs previously abandoned due to their recognized toxicity, such as colistin. However, the need to avoid kidney toxicity from colistin has led to its topical use. Read More

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http://dx.doi.org/10.12890/2019_000996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372045PMC
January 2019

Current trends in the treatment of pneumonia due to multidrug-resistant Gram-negative bacteria.

F1000Res 2019 30;8. Epub 2019 Jan 30.

Department of Medicine, University of North Carolina, Chapel Hill, NC, 27514, USA.

Pneumonia is one of the most common infections worldwide. Morbidity, mortality, and healthcare costs increase substantially when pneumonia is caused by multidrug-resistant Gram-negative bacteria (MDR-GNB). The ongoing spread of antimicrobial resistance has made treating MDR-GNB pneumonia increasingly difficult. Read More

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http://dx.doi.org/10.12688/f1000research.16517.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6354321PMC
January 2019

Epidemiology and risk factors for nosocomial infection in the respiratory intensive care unit of a teaching hospital in China: A prospective surveillance during 2013 and 2015.

BMC Infect Dis 2019 Feb 12;19(1):145. Epub 2019 Feb 12.

Department of Respiratory Intensive Care Unit of The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

Background: To determine the epidemiology and risk factors for nosocomial infection (NI) in the Respiratory Intensive Care Unit (RICU) of a teaching hospital in Northwest China.

Methods: An observational, prospective surveillance was conducted in the RICU from 2013 to 2015. The overall infection rate, distribution of infection sites, device-associated infections and pathogen in the RICU were investigated. Read More

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http://dx.doi.org/10.1186/s12879-019-3772-2DOI Listing
February 2019
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Utility of Combination Antimicrobial Therapy in Adults with Bloodstream Infections due to Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Based on In Vitro Analysis at Two Community Hospitals.

Antibiotics (Basel) 2019 Feb 8;8(1). Epub 2019 Feb 8.

Department of Clinical Pharmacy and Outcomes Sciences, University of South Carolina College of Pharmacy, Columbia, SC 29208, USA.

This study examined the utility of combination therapy for bloodstream isolates of and non-fermenting Gram-negative bacilli (NFGN) from adults at two community hospitals from January 2010 through to June 2015. Changes to in vitro antimicrobial susceptibilities by adding ciprofloxacin or gentamicin to third-generation cephalosporins (3GC) were examined overall and in patients with risk factors for 3GC resistance. Overall ceftriaxone susceptibility among was 996/1063 (94%) and 247/295 (84%) in patients with 3GC resistance risk factors. Read More

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http://dx.doi.org/10.3390/antibiotics8010015DOI Listing
February 2019

Efficacy of intravesical targeting of novel quorum sensing inhibitor nanoparticles against Pseudomonas aeruginosa biofilm-associated murine pyelonephritis.

J Drug Target 2019 Feb 11:1-9. Epub 2019 Feb 11.

a Department of Microbiology , Panjab University , Chandigarh , India.

Pseudomonas aeruginosa biofilm-associated pyelonephritis is a severe infection that can lead to mortality. There are no strategies that can effectively manage this infection since the pathogenesis is controlled by quorum sensing (QS) regulated virulence and recalcitrant biofilms. QS inhibitors (QSIs) are emerging therapeutics against such infections but are associated with cytotoxicity or low bioactivity. Read More

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http://dx.doi.org/10.1080/1061186X.2019.1574802DOI Listing
February 2019

Piperacillin pharmacokinetics and target attainment in children with cancer and fever: Can we optimize our dosing strategy?

Pediatr Blood Cancer 2019 Feb 10:e27654. Epub 2019 Feb 10.

Department of Pediatrics and Adolescent Medicine, Aarhus University Hospital, Aarhus, Denmark.

Background: Data on piperacillin-tazobactam pharmacokinetics and optimal dosing in children with cancer and fever are limited. Our objective was to investigate piperacillin pharmacokinetics and the probability of target attainment (PTA) with standard intermittent administration (IA), and to simulate PTA in other dosing regimens.

Procedure: This prospective pharmacokinetic study was conducted from April 2016 to January 2018. Read More

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http://dx.doi.org/10.1002/pbc.27654DOI Listing
February 2019
1 Read

Pharmacodynamics of ciprofloxacin against Pseudomonas aeruginosa planktonic and biofilm-derived cells.

Lett Appl Microbiol 2019 Feb 11. Epub 2019 Feb 11.

Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, Coldharbour Lane, Bristol, BS16 1QY, England, United Kingdom.

The influence of growth phase and state on survival and recovery of Pseudomonas aeruginosa exposed to ciprofloxacin was investigated using batch culture grown planktonic cells and disaggregated biofilm populations. Biofilms were either non-antibiotic exposed or previously exposed to ciprofloxacin before disaggregation and subsequent challenge with ciprofloxacin. Viable counts showed that late stationary phase cells were tolerant to ciprofloxacin over 24 h exposure, whilst all other populations presented a biphasic killing pattern. Read More

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http://dx.doi.org/10.1111/lam.13126DOI Listing
February 2019

Studies on the inhibition of AmpC and other β-lactamases by cyclic boronates.

Biochim Biophys Acta Gen Subj 2019 Feb 7;1863(4):742-748. Epub 2019 Feb 7.

Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom. Electronic address:

Background: The β-lactam antibiotics represent the most successful drug class for treatment of bacterial infections. Resistance to them, importantly via production of β-lactamases, which collectively are able to hydrolyse all classes of β-lactams, threatens their continued widespread use. Bicyclic boronates show potential as broad spectrum inhibitors of the mechanistically distinct serine- (SBL) and metallo- (MBL) β-lactamase families. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.004DOI Listing
February 2019
2 Reads

Using the best available data to estimate the cost of antimicrobial resistance: a systematic review.

Antimicrob Resist Infect Control 2019 1;8:26. Epub 2019 Feb 1.

4University of Chichester, West Sussex, UK.

Background: Valuation of the economic cost of antimicrobial resistance (AMR) is important for decision making and should be estimated accurately. Highly variable or erroneous estimates may alarm policy makers and hospital administrators to act, but they also create confusion as to what the most reliable estimates are and how these should be assessed. This study aimed to assess the quality of methods used in studies that quantify the costs of AMR and to determine the best available evidence of the incremental cost of these infections. Read More

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http://dx.doi.org/10.1186/s13756-019-0472-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359818PMC
February 2019
1 Read

Evolutionary highways to persistent bacterial infection.

Nat Commun 2019 Feb 7;10(1):629. Epub 2019 Feb 7.

Department of Clinical Microbiology, Rigshospitalet, 2100, Copenhagen Ø, Denmark.

Persistent infections require bacteria to evolve from their naïve colonization state by optimizing fitness in the host via simultaneous adaptation of multiple traits, which can obscure evolutionary trends and complicate infection management. Accordingly, here we screen 8 infection-relevant phenotypes of 443 longitudinal Pseudomonas aeruginosa isolates from 39 young cystic fibrosis patients over 10 years. Using statistical modeling, we map evolutionary trajectories and identify trait correlations accounting for patient-specific influences. Read More

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http://dx.doi.org/10.1038/s41467-019-08504-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367392PMC
February 2019

Study the antibacterial and antibiofilm activity of Carum copticum against antibiotic-resistant bacteria in planktonic and biofilm forms.

Microb Pathog 2019 Feb 4;129:99-105. Epub 2019 Feb 4.

Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

Objectives: Microbial biofilms has attracted interest in the recent years because they has become the most important cause of nosocomial infections. This study was aimed to examine the antibacterial activities of Carum copticum extracts on the development of microbial biofilms and planktonic form of six pathogenic bacteria.

Methods: Antimicrobial activity of the crude extracts against the planktonic form of six pathogenic bacteria: Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii and Klebsiella pneumonia was evaluated by using the disc diffusion method. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.02.002DOI Listing
February 2019
2 Reads

Inhibition of and Biofilm and Virulence by Active Fraction of (L.) Skeels Leaf Extract: In-Vitro and In Silico Studies.

Indian J Microbiol 2019 Mar 6;59(1):13-21. Epub 2018 Dec 6.

Department of Molecular Biology and Biotechnology, Tezpur University (A Central University), Napaam, Tezpur, Assam 784028 India.

L. Skeels (Myretacae family) is a native plant of the Indian subcontinent which has wide socio-economical importance and is well known for its ant diabetic activity. The present study aimed to investigate the antibiofilm activity of purified fraction (EA) from leaf extract against and . Read More

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http://dx.doi.org/10.1007/s12088-018-0770-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328409PMC

Phage therapy against Pseudomonas aeruginosa infections in a cystic fibrosis zebrafish model.

Sci Rep 2019 Feb 6;9(1):1527. Epub 2019 Feb 6.

Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano - LITA, via Fratelli Cervi 93, 20090, Segrate, MI, Italy.

Cystic fibrosis (CF) is a hereditary disease due to mutations in the CFTR gene and causes mortality in humans mainly due to respiratory infections caused by Pseudomonas aeruginosa. In a previous work we used phage therapy, which is a treatment with a mix of phages, to actively counteract acute P. aeruginosa infections in mice and Galleria mellonella larvae. Read More

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http://www.nature.com/articles/s41598-018-37636-x
Publisher Site
http://dx.doi.org/10.1038/s41598-018-37636-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6365511PMC
February 2019
2 Reads

Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Isolated from Burn Patients in Tehran, Iran.

Infect Disord Drug Targets 2019 Feb 6. Epub 2019 Feb 6.

Student Research Committee, Qazvin University of Medical Sciences, Qazvin. Iran.

Introduction: Plasmid-induced quinolone resistance has raised a great concern in the treatment of serious infections worldwide. The aims of this study were to determine the antibiotic susceptibility, the frequency of qepA, aac(6')-Ib, and qnr genes by PCR and sequencing, and typing of the resistant isolates using repetitive extragenic palindromic sequence-based PCR (REP-PCR) in Pseudomonas aeruginosa isolated from burn wound infections.

Material And Methods: In the current cross-sectional study, 149 P. Read More

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http://dx.doi.org/10.2174/1871526519666190206205521DOI Listing
February 2019
13 Reads

Anti-Virulence Strategy against the Multidrug-Resistant Bacterial Pathogen Pseudomonas aeruginosa: Pseudolysin (Elastase B) as a Potential Druggable Target.

Curr Protein Pept Sci 2019 Feb 6. Epub 2019 Feb 6.

Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro. Brazil.

Pseudomonas aeruginosa is a non-fermentative, gram-negative bacterium that is one of the most common pathogens responsible for hospital-acquired infections worldwide. The management of the infections caused by P. aeruginosa represents a huge challenge in healthcare settings due to the increased emergence of resistant isolates, some of them resistant to all the currently available antimicrobials, which results in elevated morbimortality rates. Read More

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http://dx.doi.org/10.2174/1389203720666190207100415DOI Listing
February 2019

A benzimidazole-based ruthenium(IV) complex inhibits Pseudomonas aeruginosa biofilm formation by interacting with siderophores and the cell envelope, and inducing oxidative stress.

Biofouling 2019 Feb 7:1-16. Epub 2019 Feb 7.

a Department of Microbiology, Institute of Biology , Jan Kochanowski University in Kielce , Poland.

Pseudomonas aeruginosa biofilm-associated infections are a serious medical problem, and new compounds and therapies acting through novel mechanisms are much needed. Herein, the authors report a ruthenium(IV) complex that reduces P. aeruginosa PAO1 biofilm formation by 84%, and alters biofilm morphology and the living-to-dead cell ratio at 1 mM concentration. Read More

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http://dx.doi.org/10.1080/08927014.2018.1564818DOI Listing
February 2019

Biofilm inhibition and anti-quorum sensing activity of phytosynthesized silver nanoparticles against the nosocomial pathogen Pseudomonas aeruginosa.

Biofouling 2019 Feb 7:1-16. Epub 2019 Feb 7.

a Microbial Diversity Research Centre , Dr. D. Y. Patil Biotechnology and Bioinformatics Institute , Dr. D. Y. Patil Vidyapeeth , Pune , India.

Quorum sensing (QS), the communication signaling network, regulates biofilm formation and several virulence factors in Pseudomonas aeruginosa PAO1, a nosocomial opportunistic pathogen. QS is considered to be a challenging target for compounds antagonistic to virulent factors. Biologically synthesized silver nanoparticles (AgNPs) are reported as anti-QS and anti-biofilm drugs against bacterial infections. Read More

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https://www.tandfonline.com/doi/full/10.1080/08927014.2018.1
Publisher Site
http://dx.doi.org/10.1080/08927014.2018.1563686DOI Listing
February 2019
6 Reads

Plazomicin: A Novel Aminoglycoside for the Treatment of Resistant Gram-Negative Bacterial Infections.

Drugs 2019 Feb 6. Epub 2019 Feb 6.

Department of Pharmacy Services, Sinai-Grace Hospital, Detroit Medical Center, Wayne State University of School of Medicine, Detroit, MI, USA.

Plazomicin is a novel semisynthetic parenteral aminoglycoside that inhibits bacterial protein synthesis. It was approved by the United States Food and Drug Administration for use in adults with complicated urinary tract infections (cUTI), including pyelonephritis. Plazomicin displays potent in vitro activity against Enterobacteriaceae, including both extended-spectrum β-lactamase-producing and carbapenem-resistant isolates. Read More

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http://dx.doi.org/10.1007/s40265-019-1054-3DOI Listing
February 2019
4.343 Impact Factor

Influence of poly-L-lysine molecular weight on antibacterial efficacy in polymer multilayer films.

J Biomed Mater Res A 2019 Feb 4. Epub 2019 Feb 4.

School of Engineering, Brown University, Providence, RI.

Antibacterial coatings can prevent and treat medical device-associated infections. We examined the antibacterial properties of coatings assembled from poly-L-lysine (PLL) and hyaluronic acid (HA). (PLL/HA) films were fabricated using layer-by-layer assembly with three different PLL MWs, differentiated by number of repeat units, i. Read More

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http://dx.doi.org/10.1002/jbm.a.36645DOI Listing
February 2019

Antibacterial Potential of (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains.

Int J Microbiol 2019 2;2019:5328238. Epub 2019 Jan 2.

School of Pharmacy, College of Medicine and Health Science, Wollo University, Dessie, Ethiopia.

Objective: To evaluate the antibacterial effects of the leaf latex of against infectious bacterial strains.

Methods: The leaf latex of at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as (ATCC 14700) and (ATCC 35619) and Gram-positive such as (ATCC 50080) and (ATCC 4623).

Results: The tested concentrations of the latex ranging between 400 and 600 mg·mL showed significant antibacterial activity against bacterial strain. Read More

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http://dx.doi.org/10.1155/2019/5328238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334358PMC
January 2019

Can Inhibit Growth of Streptococcal Species via Siderophore Production.

J Bacteriol 2019 Feb 4. Epub 2019 Feb 4.

Department of Microbiology and Immunology, v, Hanover, NH 03755

Cystic Fibrosis (CF) is a genetic disease that causes patients to accumulate thick, dehydrated mucus in the lung and develop chronic, polymicrobial infections due to reduced mucociliary clearance. These chronic polymicrobial infections and subsequent decline in lung function are significant factors in the morbidity and mortality of CF. and spp. Read More

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http://dx.doi.org/10.1128/JB.00014-19DOI Listing
February 2019
3 Reads

Activity of ceftolozane-tazobactam and comparators when tested against Gram-negative isolates collected from paediatric patients in the United States and Europe during 2012-2016 as part of a global surveillance programme.

Int J Antimicrob Agents 2019 Feb 1. Epub 2019 Feb 1.

JMI Laboratories, North Liberty, Iowa, USA.

Ceftolozane-tazobactam (C-T) is a combination of an antipseudomonal cephalosporin and a β-lactamase inhibitor. C-T was approved by the US Food and Drug Administration in 2014 and by the European Medicine Agency in 2015 for use in adults to treat complicated urinary tract infections, acute pyelonephritis, and complicated intra-abdominal infections with metronidazole. Studies for paediatric indications are planned. Read More

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http://dx.doi.org/10.1016/j.ijantimicag.2019.01.015DOI Listing
February 2019
1 Read

The effects of novel heme oxygenase inhibitors on the growth of Pseudomonas aeruginosa.

Microb Pathog 2019 Feb 1;129:64-67. Epub 2019 Feb 1.

Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD 21201, United States. Electronic address:

Objectives: This study was aimed to develop small molecule inhibitors of the P. aeruginosa heme oxygenase (pa-HemO) as potential treatment of infections caused by P. aeruginosa. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.01.047DOI Listing
February 2019

Production, characterization, and powder preparation of quorum quenching acylase AiiO for pathogen control.

Prep Biochem Biotechnol 2019 Feb 3:1-7. Epub 2019 Feb 3.

a School of Biological Engineering, Dalian Polytechnic University , Dalian , China.

Acylase AiiO is a novel quorum quenching enzyme with a broad substrate spectrum of acyl-homoserine lactones (AHLs) and has promising prospects in pathogen control. In this work, acylase AiiO production by a recombinant E. coli strain and its characterization were investigated; the acylase powder was further prepared and evaluated for effectiveness. Read More

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http://dx.doi.org/10.1080/10826068.2018.1550657DOI Listing
February 2019
1 Read

Cefiderocol: A Siderophore Cephalosporin with Activity Against Carbapenem-Resistant and Multidrug-Resistant Gram-Negative Bacilli.

Drugs 2019 Feb 2. Epub 2019 Feb 2.

Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.

Cefiderocol is an injectable siderophore cephalosporin discovered and being developed by Shionogi & Co., Ltd., Japan. Read More

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http://dx.doi.org/10.1007/s40265-019-1055-2DOI Listing
February 2019
2 Reads

Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients.

J Cyst Fibros 2019 Jan 30. Epub 2019 Jan 30.

Department of Clinical Microbiology, Rigshospitalet, 2100 Copenhagen, Denmark; Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health Sciences University of Copenhagen, 2200 Copenhagen, Denmark. Electronic address:

Background: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Read More

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http://dx.doi.org/10.1016/j.jcf.2019.01.005DOI Listing
January 2019

Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS.

Mol Microbiol 2019 Feb 2. Epub 2019 Feb 2.

Department of Biological Sciences, Binghamton University, Binghamton, NY, 13902, United States.

The hybrid sensor SagS plays a central role in the formation of P. aeruginosa biofilms, by enabling the switch from the planktonic to the biofilm mode of growth, and by facilitating the transition of biofilm cells to a highly tolerant state. In this study, we examined the importance of the SagS key amino acid residues associated with biofilm formation (L154) and antibiotic tolerance (D105) in P. Read More

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http://dx.doi.org/10.1111/mmi.14215DOI Listing
February 2019
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ESCMID-EUCIC clinical guidelines on decolonisation of multidrug-resistant Gram-negative bacteria carriers.

Clin Microbiol Infect 2019 Jan 29. Epub 2019 Jan 29.

Division of Infectious Diseases, Microbiology and Preventive Medicine, Hospital Universitario Virgen Macarena / Department of Medicine, University of Seville / Biomedicine Institute of Seville (IBiS), Seville, Spain.

Scope: The aim of these guidelines is to provide recommendations for decolonising regimens targeting multidrug-resistant Gram-negative bacteria (MDR-GNB) carriers in all settings.

Methods: These evidence-based guidelines were produced after a systematic review of published studies on decolonisation interventions targeting the following MDR-GNB: third-generation cephalosporin-resistant Enterobacteriaceae (3GCephRE), carbapenem-resistant Enterobacteriaceae (CRE), aminoglycoside-resistant Enterobacteriaceae (AGRE), fluoroquinolone-resistant Enterobacteriaceae (FQRE), extremely drug-resistant Pseudomonas aeruginosa (XDRPA), carbapenem-resistant Acinetobacter baumannii (CRAB), cotrimoxazole-resistant Stenotrophomonas maltophilia (CRSM), colistin-resistant Gram-negative organisms (CoRGNB), pan-drug-resistant Gram-negative organisms (PDRGNB). The recommendations are grouped by MDR-GNB species. Read More

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http://dx.doi.org/10.1016/j.cmi.2019.01.005DOI Listing
January 2019

A multiplexed, indirect enzyme-linked immunoassay for the detection and differentiation of E. coli from other Enterobacteriaceae and P. aeruginosa from other glucose non-fermenters.

J Microbiol Methods 2019 Jan 29;158:52-58. Epub 2019 Jan 29.

School of Public Health, Division of Infectious Disease and Vaccinology, University of California, Berkeley, CA 94720, USA. Electronic address:

Gram-negative bacteria (GNB) are important causes of community (CA) and hospital (HA)- associated infections. Here we describe the development of an indirect ELISA (I-ELISA), which can be used to detect and differentiate the Enterobacteriaceae Escherichia coli, and glucose non-fermenter Pseudomonas aeruginosa from other GNB species. The I-ELISA utilizes six antibodies for bacterial speciation, which were grouped according to their bacterial targets; Enterobacteriaceae (SL-EntA and CH1810 mAb), Escherichia coli (SL-EcA and 6103-46 mAb), Pseudomonas aeruginosa (SL-PaA and SL-PaB). Read More

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http://dx.doi.org/10.1016/j.mimet.2019.01.014DOI Listing
January 2019
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Secretory IgA-mediated immune response in saliva and early detection of Pseudomonas aeruginosa in the lower airways of pediatric cystic fibrosis patients.

Med Microbiol Immunol 2019 Jan 31. Epub 2019 Jan 31.

Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Rua Vital Brasil 251, 2nd floor, Cidade Universitária, Barão Geraldo, Campinas, SP, 13083-888, Brazil.

Pseudomonas aeruginosa (Pa) detection in the paranasal sinuses may help to prevent or postpone bacterial aspiration to the lower airways (LAW) and chronic lung infection in cystic fibrosis (CF). We assessed the ability of an ELISA test for measurement of specific Pa secretory IgA (sIgA) in saliva (a potential marker of sinus colonization) to early detect changes in the Pa LAW status (indicated by microbiological sputum or cough swab culture and specific serum IgG levels) of 65 patients for three years, in different investigation scenarios. Increased sIgA levels were detected in saliva up to 22 months before changes in culture/serology. Read More

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http://dx.doi.org/10.1007/s00430-019-00578-wDOI Listing
January 2019
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Direct identification of pathogens in urine using a specific MALDI-TOF spectra database.

J Clin Microbiol 2019 Jan 30. Epub 2019 Jan 30.

IHU-Méditerranée Infection, Marseille, France

Urinary tract infections are among the most common reasons for antimicrobial treatment, early diagnosis could have a significant impact by enabling rapid administration of the adapted antibiotic and preventing complications. The current delay between sample receipt and pathogen identification is about 24-48 hours, which could be significantly shortened by an accurate direct method. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is already used for the identification of pathogens in clinical laboratories and constitutes a promising tool for direct diagnosis. Read More

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http://dx.doi.org/10.1128/JCM.01678-18DOI Listing
January 2019
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A multi-faceted approach testing the effects of previous bacterial exposure on resistance and tolerance.

J Anim Ecol 2019 Jan 29. Epub 2019 Jan 29.

Institute for Evolution and Biodiversity, University of Münster, Hüfferstrasse 1, 48149, Münster, Germany.

1.Hosts can alter their strategy towards pathogens during their lifetime, i.e. Read More

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http://dx.doi.org/10.1111/1365-2656.12953DOI Listing
January 2019

Antimicrobial susceptibility and virulence genes of clinical and environmental isolates of .

PeerJ 2019 22;7:e6217. Epub 2019 Jan 22.

Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Background: is ubiquitous, has intrinsic antibiotic resistance mechanisms, and is associated with serious hospital-associated infections. It has evolved from being a burn wound infection into a major nosocomial threat. In this study, we compared and correlated the antimicrobial resistance, virulence traits and clonal relatedness between clinical and fresh water environmental isolates of . Read More

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https://peerj.com/articles/6217
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http://dx.doi.org/10.7717/peerj.6217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346980PMC
January 2019
5 Reads

New insights into culture negative endophthalmitis by unbiased next generation sequencing.

Sci Rep 2019 Jan 29;9(1):844. Epub 2019 Jan 29.

Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.

The proof-of-concept, study to investigate the presence of microorganisms in presumed infectious endophthalmitis using Next generation sequencing (NGS) was carried out in vitreous biopsies from 34 patients with endophthalmitis, and thirty patients undergoing surgery for non-infectious retinal disorders as controls. Following DNA extraction using the Qiagen mini kit and PCR amplification of the V3-V4 regions of the bacterial 16S rRNA and ITS 2 region of fungus, they samples were sequenced on an Illumina HiSeq 2500 Machine. Paired reads were curated, taxonomically labeled, and filtered. Read More

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http://www.nature.com/articles/s41598-018-37502-w
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http://dx.doi.org/10.1038/s41598-018-37502-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351655PMC
January 2019
3 Reads

Competition in Biofilms between Cystic Fibrosis Isolates of Is Shaped by R-Pyocins.

MBio 2019 Jan 29;10(1). Epub 2019 Jan 29.

School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA

is an opportunistic pathogen and the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. infections are difficult to treat due to a number of antibiotic resistance mechanisms and the organism's propensity to form multicellular biofilms. Epidemic strains of often dominate within the lungs of individual CF patients, but how they achieve this is poorly understood. Read More

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http://dx.doi.org/10.1128/mBio.01828-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6355985PMC
January 2019

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections.

J Leukoc Biol 2019 Jan 28. Epub 2019 Jan 28.

Department of Medicine, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Pseudomonas aeruginosa-induced corneal keratitis is a sight-threatening disease. The rise of antibiotic resistance among P. aeruginosa keratitis isolates makes treatment of this disease challenging, emphasizing the need for alternative therapeutic modalities. Read More

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http://dx.doi.org/10.1002/JLB.4HI0918-364RRDOI Listing
January 2019

Antibiofilm Nitric Oxide-Releasing Polydopamine Coatings.

ACS Appl Mater Interfaces 2019 Feb 8. Epub 2019 Feb 8.

Future Industries Institute , The University of South Australia , Mawson Lakes , SA 5095 , Australia.

The growing number of patient morbidity related to nosocomial infections has placed an importance on the development of new antibacterial coatings for medical devices. Here, we utilize the versatile adhesion property of polydopamine (pDA) to design an antibacterial coating that possesses low-fouling and nitric oxide (NO)-releasing capabilities. To demonstrate this, glass substrates were functionalized with pDA via immersion in alkaline aqueous solution containing dopamine, followed by grafting of low-fouling polymer (poly(ethylene glycol) (PEG)) via Michael addition and subsequent formation of N-diazeniumdiolate functionalities (NO precursors) by purging with NO gas. Read More

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http://pubs.acs.org/doi/10.1021/acsami.8b16853
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http://dx.doi.org/10.1021/acsami.8b16853DOI Listing
February 2019
7 Reads
6.723 Impact Factor

Genome-Based Approach Delivers Vaccine Candidates Against .

Front Immunol 2018 9;9:3021. Epub 2019 Jan 9.

Infection and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

High incidence, severity and increasing antibiotic resistance characterize infections, highlighting the need for new therapeutic options. Vaccination strategies to prevent or limit infections represent a rational approach to positively impact the clinical outcome of risk patients; nevertheless this bacterium remains a challenging vaccine target. To identify novel vaccine candidates, we started from the genome sequence analysis of the reference strain PAO1 exploring the reverse vaccinology approach integrated with additional bioinformatic tools. Read More

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https://www.frontiersin.org/article/10.3389/fimmu.2018.03021
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http://dx.doi.org/10.3389/fimmu.2018.03021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334337PMC
January 2019
4 Reads

Novel Pyoverdine Inhibitors Mitigate Pathogenesis.

Front Microbiol 2018 9;9:3317. Epub 2019 Jan 9.

Department of BioSciences, Rice University, Houston, TX, United States.

is a clinically important pathogen that causes a variety of infections, including urinary, respiratory, and other soft-tissue infections, particularly in hospitalized patients with immune defects, cystic fibrosis, or significant burns. Antimicrobial resistance is a substantial problem in treatment due to the inherent insensitivity of the pathogen to a wide variety of antimicrobial drugs and its rapid acquisition of additional resistance mechanisms. One strategy to circumvent this problem is the use of anti-virulent compounds to disrupt pathogenesis without directly compromising bacterial growth. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2018.03317
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http://dx.doi.org/10.3389/fmicb.2018.03317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333909PMC
January 2019
2 Reads