108,717 results match your criteria Pseudomonas Aeruginosa Infections


Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico.

World J Microbiol Biotechnol 2018 Dec 15;35(1). Epub 2018 Dec 15.

Centro de Investigación en Alimentación y Desarrollo, A.C. Carr. Gustavo Enrique Astiazarán No. 46 AP 1735, CP 83000, Hermosillo, Sonora, Mexico.

The aim of this research was to study the hydrolytic potential of bacteria isolated from cattle environments of two desert soils in one of the driest and hottest zones in America. A total of 26 points were sampled, 144 strains were isolated, and 50 strains were selected for the characterization of esterase, lipase, protease, and amylase activities and for 16S rRNA identification. Strains of the Bacillus, Pseudomonas, Acinetobacter, Enterobacter, Providencia, Escherichia, and Pantoea genera were identified. Read More

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December 2018

Bacterial exopolysaccharide promotes acid tolerance in Bacillus amyloliquefaciens and improves soil aggregation.

Mol Biol Rep 2018 Dec 15. Epub 2018 Dec 15.

Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India.

In this paper we report the isolation and taxonomic characterization of exopolysaccharide (EPS) producing bacteria followed by the role of EPS in conferring acid tolerance to the soil bacteria Bacillus amyloliquefaciens p16. The role of EPS in promoting soil aggregation is also presented. A total of 75 isolates were tested for acid tolerance and biofilm production under acid stress of which, 54 isolates were further tested for EPS production. Read More

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December 2018

Microbiological surveillance of flexible bronchoscopes after a high-level disinfection with peracetic acid: preliminary results from an Italian teaching hospital.

Ann Ig 2019 Jan-Feb;31(1):13-20

Hygiene and Health Organization, Hospital "Careggi", Florence, Italy.

Background: Flexible bronchoscopes are heat labile, complex and difficult to clean, and some nosocomial outbreaks related to bronchoscopy have been reported in literature. The aim of our study was to determine, through a systematic monitoring, whether bronchoscopes' cleaning and disinfection procedures have been correctly adopted by health operators.

Methods: We conducted a 19 months-long prospective study in the Unit of Pulmonology at Careggi Teaching Hospital (Florence, Italy), analyzing endoscopes that were reprocessed through a high-level disinfection procedure. Read More

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December 2018

Investigating the effect of copper and magnesium ions on nitrogen removal capacity of pure cultures by modified non-competitive inhibition model.

Ecotoxicol Environ Saf 2018 Dec 13;170:479-487. Epub 2018 Dec 13.

Chongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, China. Electronic address:

Copper, a common heavy metal, may be beneficial for or poisonous to microbial activity. The objective of this study was to determine the effect of different copper ion concentrations on the nitrogen removal performance of Arthrobacter arilaitensis strain Y-10 and Pseudomonas taiwanensis strain J488. The non-competitive inhibition model was employed to evaluate the 50% inhibition concentrations (IC values) of copper ions toward the pure strains. Read More

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December 2018

Development of modified montmorillonite-bacterial cellulose nanocomposites as a novel substitute for burn skin and tissue regeneration.

Carbohydr Polym 2019 Feb 13;206:548-556. Epub 2018 Nov 13.

Biotechnology Program, Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus 22060, Pakistan. Electronic address:

Bacterial cellulose (BC) is a promising biopolymer with wound healing and tissue regenerative properties but lack of antimicrobial property limits its biomedical applications. Therefore, current study was proposed to combine wound healing property of BC with antimicrobial activity of montmorillonite (MMT) and modified montmorillonites (Cu-MMT, Na-MMT and Ca-MMT) to design novel artificial substitute for burns. Designed nanocomposites were characterized through Fe-SEM, FTIR and XRD. Read More

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February 2019
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Inhibition of bacterial adhesion and biofilm formation of sulfonated chitosan against Pseudomonas aeruginosa.

Carbohydr Polym 2019 Feb 10;206:412-419. Epub 2018 Nov 10.

Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huaian 223003, PR China.

The exploitation of chitosan derivatives as safe and natural polymers against microorganisms has been reconsidered as an alternative to antibiotics and chemical preservatives. Herein, inhibition of bacterial adhesion and biofilm formation by anionic chitosan (sulfonated chitosan, SCS) and cationic chitosan (chitosan hydrochloride, WCS) against P. aeruginosa were investigated. Read More

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February 2019

Smart wound dressing based on κ-carrageenan/locust bean gum/cranberry extract for monitoring bacterial infections.

Carbohydr Polym 2019 Feb 12;206:362-370. Epub 2018 Nov 12.

Pós-Graduação em Ciências da Saúde, Universidade do Sul de Santa Catarina, 88701-000, Tubarão, SC, Brazil.

A smart wound dressing based on carrageenan (κC), locust bean gum (LBG), and cranberry extract (CB) for monitoring bacterial wound infections was developed and characterized using UV-vis spectroscopy, FT-IR, and SEM. The mechanical, swelling, cytotoxic and pH sensor properties were also investigated. UV-vis spectra demonstrated that the obtained κC:LBG:CB hydrogel film exhibited a visible change of colors as it was immersed in PBS solution pH 5. Read More

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February 2019

Phage-based assay for rapid detection of bacterial pathogens in blood by Raman spectroscopy.

J Immunol Methods 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. Electronic address:

Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. Read More

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December 2018
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An important role of -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity.

New Phytol 2018 Dec 15. Epub 2018 Dec 15.

Department of Energy Plant Research Laboratory, East Lansing, MI, 48824, USA.

Plants mount coordinated immune responses to defend against pathogens. However, the cellular components required for plant immunity are not fully understood. The jasmonate-mimicking coronatine (COR) toxin produced by Pseudomonas syringae pv. Read More

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December 2018

In Schizophrenia, Increased Plasma IgM/IgA Responses to Gut Commensal Bacteria Are Associated with Negative Symptoms, Neurocognitive Impairments, and the Deficit Phenotype.

Neurotox Res 2018 Dec 15. Epub 2018 Dec 15.

Department of Psychiatry, University of Toronto, Toronto, ON, Canada.

Increased gut permeability (leaky gut) with increased translocation of Gram-negative bacteria plays a role in the gut-brain axis through effects on systemic immune-inflammatory processes. Deficit schizophrenia is characterized by an immune-inflammatory response combined with a deficit in natural IgM antibodies to oxidative-specific epitopes (OSEs), which are a first-line defense against bacterial infections. This study measured plasma IgA/IgM responses to 5 Gram-negative bacteria in association with IgM responses to malondialdehyde (MDA) and azelaic acid in 80 schizophrenia patients (40 with the deficit syndrome and 40 without) and in 38 healthy controls. Read More

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December 2018

Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea.

Authors:
Kwan Soo Ko

J Microbiol 2018 Oct 2. Epub 2018 Oct 2.

Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.

Antibiotic resistance is a global concern in public health. Antibiotic-resistant clones can spread nationally, internationally, and globally. This review considers representative antibiotic-resistant Gram-negative bacterial clones-CTX-M- 15-producing ST131 in Escherichia coli, extended-spectrum β-lactamase-producing ST11 and KPC-producing ST258 in Klebsiella pneumoniae, IMP-6-producing, carbapenem-resistant ST235 in Pseudomonas aeruginosa, and OXA-23-producing global clone 2 in Acinetobacter baumannii-that have disseminated worldwide, including in Korea. Read More

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October 2018

Radiologic types of Mycobacterium xenopi pulmonary disease: different patients with similar short-term outcomes.

Eur J Clin Microbiol Infect Dis 2018 Dec 14. Epub 2018 Dec 14.

Division of Respirology, Department of Medicine, University of Toronto, Toronto, ON, Canada.

Mycobacterium xenopi pulmonary disease (Mxe-PD) is common among nontuberculous mycobacterial infections in Europe and Canada. Associations between radiological pattern and clinical features and outcomes are inadequately studied in Mxe-PD. We sought to investigate clinical characteristics and outcomes according to the dominant radiological pattern among patients with Mxe-PD. Read More

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December 2018

A review on mechanism of action, resistance, synergism, and clinical implications of mupirocin against Staphylococcus aureus.

Biomed Pharmacother 2019 Jan 26;109:1809-1818. Epub 2018 Nov 26.

Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Mupirocin (MUP), bactroban, or pseudomonic acid is a natural crotonic acid derivative drug extracted from Pseudomonas fluorescens which is produced by modular polyketide synthases. This antibiotic has a unique chemical structure and mechanism of action. It is a mixture of A-D pseudomonic acids and inhibits protein synthesis through binding to bacterial isoleucyl-tRNA synthetase. Read More

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January 2019
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Danshen can interact with intestinal bacteria from normal and chronic renal failure rats.

Biomed Pharmacother 2019 Jan 26;109:1758-1771. Epub 2018 Nov 26.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Danshen (Salviae Miltiorrhizae Radix et Rhizoma, SMR) has been used as a traditional Chinese medicine in clinic for treatment of coronary heart diseases. Previous works have shown that the chronic renal failure (CRF) is closely related to changes of intestinal bacteria. The aim is to explore the interaction between active components of SMR and intestinal bacteria from normal and CRF rats. Read More

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January 2019
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Assembled gold nanorods for the photothermal killing of bacteria.

Colloids Surf B Biointerfaces 2019 Jan 23;173:833-841. Epub 2018 Oct 23.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China. Electronic address:

Titanium and its alloys are widely used in many clinical applications, but implant-associated infection may lead to implant failure. Because of the increasing concern about antibiotic resistant pathogen, photothermal therapy (PTT) as a new treatment strategy has received considerable attention. In this work, gold nanorods (GNRs) photoexcited by the near-infrared (NIR) light were immobilized on Ti surface by electrostatic surface self-assembly technique. Read More

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January 2019

Effect of cosmetic chemical preservatives on resident flora isolated from healthy facial skin.

J Cosmet Dermatol 2018 Dec 12. Epub 2018 Dec 12.

Beijing Key Lab of Plant Resources Research and Development, College of Science, Beijing Technology and Business University, Beijing, China.

Background/aims: Healthy skin harbors numerous microbes known to maintain its health and prevent attacks from external pathogens. The influence of chemical preservatives commonly used in cosmetic products on facial resident flora remains poorly characterized. In this study, we aimed to investigate the antibacterial activity of five such preservatives on in vitro cultivated skin-resident bacteria. Read More

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December 2018
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In vitro antimicrobial activity of seven adjuvants against common pathogens associated with canine otitis externa.

Vet Dermatol 2018 Dec 13. Epub 2018 Dec 13.

Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, South Australia, 5371, Australia.

Background: An antibiotic adjuvant is a chemical substance used to modify or augment the effectiveness of primary antimicrobial agents against drug-resistant micro-organisms. Its use provides an alternative approach to address the global issue of antimicrobial resistance and enhance antimicrobial stewardship.

Hypothesis/objectives: To determine the antimicrobial activity of a panel of potential antimicrobial adjuvants against common pathogens associated with canine otitis externa (OE). Read More

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December 2018
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Azetidine-Containing Alkaloids Produced by a Quorum-Sensing- Regulated Nonribosomal Peptide Synthetase Pathway in Pseudomonas aeruginosa.

Angew Chem Int Ed Engl 2018 Dec 11. Epub 2018 Dec 11.

French National Center of Scientific Research, National Museum of Natural History, Laboratory Molecules of Communication and Adaptation of Microorganisms (MCAM), CP 54, 57 rue Cuvier, 75005, Paris, FRANCE.

Pseudomonas aeruginosa displays an impressive metabolic versatility, which ensures its survival in diverse environments. Herein we report the identification of rare azetidine-containing alkaloids from P. aeruginosa PAO1, termed azetidomonamides, that are derived from a conserved, quorum-sensing regulated non-ribosomal peptide synthetase (NRPS) pathway. Read More

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December 2018

2D image quantification of microbial iron chelators (siderophores) using diffusive equilibrium in thin films method.

Anal Chem 2018 Dec 14. Epub 2018 Dec 14.

Siderophores are natural metal chelating agents that strongly control the biogeochemical metal cycles such as Fe in the environment. This article describes a new methodology to detect and quantify at the micromolar concentration the spatial distribution at millimetre scale of siderophores within the root's system. The "universal" CAS assay originally designed for bacterial siderophores detection and later designed for fungus was adapted here for diffusive equilibrium in thin film gel techniques (DET). Read More

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December 2018

Antimicrobial activity of ceftolozane-tazobactam against multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa clinical isolates from a Spanish hospital.

Rev Esp Quimioter 2018 Dec 14. Epub 2018 Dec 14.

Ana Isabel López-Calleja, Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón. 3ª planta Edificio de Consultas Externas - c/ Padre Arrupe s/n, 50009 Zaragoza, Spain.

Objective: Our objective was to evaluate the in vitro activity of ceftolozane-tazobactam against multidrug resistant (MDR) and extensively drug-resistant (XDR) non metallo-β-lactamase producing Pseudomonas aeruginosa clinical isolates at Hospital Universitario Miguel Servet (Zaragoza, Spain) from February 2016 to October 2017.

Methods: We evaluated the in vitro activity of ceftolozane-tazobactam and other antipseudomonal antibiotics against 12 MDR and 117 XDR non metallo-β-lactamase producing P. aeruginosa isolates. Read More

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December 2018
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Mechanistic Insights From Global Metabolomics Studies into Synergistic Bactericidal Effect of a Polymyxin B Combination With Tamoxifen Against Cystic Fibrosis MDR .

Comput Struct Biotechnol J 2018 10;16:587-599. Epub 2018 Nov 10.

Department of Pharmacology & Therapeutics, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville 3010, VIC, Australia.

Polymyxins are amongst the most important antibiotics in modern medicine, in recent times their clinical utility has been overshadowed by nosocomial outbreaks of polymyxin resistant MDR Gram-negative 'superbugs'. An effective strategy to surmount polymyxin resistance is combination therapy with FDA-approved non-antibiotic drugs. Herein we used untargeted metabolomics to investigate the mechanism(s) of synergy between polymyxin B and the selective estrogen receptor modulator (SERM) tamoxifen against a polymyxin-resistant MDR cystic fibrosis (CF) FADDI-PA006 isolate (polymyxin B MIC=8 mg/L , it is an MDR polymyxin resistant isolated from the lungs of a CF patient). Read More

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November 2018

Use of a Primary Epithelial Cell Screening Tool to Investigate Phage Therapy in Cystic Fibrosis.

Front Pharmacol 2018 28;9:1330. Epub 2018 Nov 28.

Telethon Kids Institute, Perth, WA, Australia.

Antimicrobial-resistant microbes are an increasing threat to human health. In cystic fibrosis (CF), airway infections with remain a key driver of lung damage. With few new antibiotics on the development horizon, alternative therapeutic approaches are needed against antimicrobial-resistant pathogens. Read More

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November 2018
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Epidemiology of bloodstream infections in patients with chronic lymphocytic leukemia: a longitudinal nation-wide cohort study.

Leukemia 2018 Dec 13. Epub 2018 Dec 13.

Department of Haematology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Patients with chronic lymphocytic leukemia (CLL) have a high risk of bloodstream infections (BSI). BSI cause significant morbidity and mortality among CLL patients; approximately one-third of fatalities in CLL list infections as cause of death. All CLL patients in Denmark diagnosed between 2008 and 2016 were followed through registries for the event of a BSI. Read More

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December 2018

Evaluation of Pseudomonas graminis CPA-7 as a biopreservation method for fresh-cut pear: Physicochemical, enzymatic, and nutritional quality.

Food Sci Technol Int 2018 Dec 13:1082013218816483. Epub 2018 Dec 13.

1 Institute of Agrifood Research and Technology, XaRTA-Postharvest, Lleida, Spain.

Biological preservation methods with bacterial antagonists have emerged as alternatives to chemical sanitizers for extending shelf-life and reducing the population of pathogenic microorganisms. In addition, calcium plays an important role in maintaining the quality of fruit, and postharvest calcium treatments might determine the potential of fruit for processing. The objective of this work was to evaluate the effect of the postharvest application of calcium and biopreservation with the CPA-7 strain of Pseudomonas graminis on the quality parameters of fresh-cut pears. Read More

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December 2018

The Effect of Organic Acid, Trisodium Phosphate and Essential Oil Component Immersion Treatments on the Microbiology of Cod () during Chilled Storage.

Foods 2018 Dec 8;7(12). Epub 2018 Dec 8.

Teagasc Food Research Centre, Ashtown, 15 Dublin, Ireland.

Spoilage is a major issue for the seafood sector with the sale and exportation of fish limited by their short shelf-life. The immediate and storage effects of immersion (30 s at 20 °C) with 5% (/) citric acid (CA), 5% (/) lactic acid (LA), 5% (/) capric acid (CP) and 12% trisodium phosphate (TSP) (experiment 1) and essential oil components (EOC) (1% (/) citral (CIT), 1% (/) carvacrol (CAR), 1% (/) thymol (THY) and 1% (/) eugenol (EUG)) (experiment 2) on the concentrations of indicator (total viable counts (TVC) (mesophilic and psychrophilic) and total counts (TEC)), and spoilage organisms ( spp., lactic acid bacteria (LAB), , spp. Read More

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December 2018

Targeting IL-13Rα2 for effective treatment of malignant peripheral nerve sheath tumors in mouse models.

Authors:

J Neurosurg 2018 Nov 1:1-11. Epub 2018 Nov 1.

OBJECTIVEMalignant peripheral nerve sheath tumors (MPNSTs) are aggressive soft tissue sarcomas that harbor a high potential for metastasis and have a devastating prognosis. Combination chemoradiation aids in tumor control and decreases tumor recurrence but causes deleterious side effects and does not extend long-term survival. An effective treatment with limited toxicity and enhanced efficacy is critical for patients suffering from MPNSTs. Read More

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November 2018

Phase I trial of convection-enhanced delivery of IL13-Pseudomonas toxin in children with diffuse intrinsic pontine glioma.

Authors:

J Neurosurg Pediatr 2018 Dec 1:1-10. Epub 2018 Dec 1.

OBJECTIVEIn this clinical trial report, the authors analyze safety and infusion distribution of IL13-Pseudomonas exotoxin, an antitumor chimeric molecule, administered via intratumoral convection enhanced delivery (CED) in pediatric patients with diffuse intrinsic pontine glioma (DIPG).METHODSThis was a Phase I single-institution, open-label, dose-escalation, safety and tolerability study of IL13-PE38QQR infused via single-catheter CED into 5 pediatric DIPG patients. IL13-PE38QQR was administered to regions of tumor selected by radiographic findings. Read More

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December 2018

Metal-tolerant endophytic bacteria associated with Silene vulgaris support the Cd and Zn phytoextraction in non-host plants.

Chemosphere 2018 Dec 5;219:250-260. Epub 2018 Dec 5.

Department of Microbiology, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.

The aim of this study was to isolate and characterise metal-resistant endophytic bacteria from the tissues of Silene vulgaris collected within the vicinity of non-ferrous steelworks in Katowice, Upper Silesia, Southern Poland. Twenty-four strains of metal-resistant endophytic bacteria that belong to 15 genera were isolated from the stems and leaves of Silene vulgaris. Most of these strains showed multiple plant growth-promoting capabilities. Read More

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December 2018
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Antibacterial Activity of Discaria americana Gillies ex Hook (Rhamnaceae).

J Ethnopharmacol 2018 Dec 10. Epub 2018 Dec 10.

Departamento de Química, Universidade Federal de Santa Maria,Santa Maria, RS, Brazil. Electronic address:

Ethnopharmacology Relevance: Discaria americana Gillies ex Hook (sin. Discaria febrifuga and Discaria longispina) (Rhamnaceae) is a plant native from Rio Grande do Sul (Southern Brazil), Uruguay and Argentine, and has been used in Brazilian traditional medicine as antipyretic agent, and for stomach disorders. In Rio Grande do Sul, Uruguay and Argentine, the roots, in decoction, are used as tonic and febrifuge. Read More

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December 2018
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Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.

Int J Biol Macromol 2018 Dec 10. Epub 2018 Dec 10.

Department of Chemistry, Payame Noor university, Tehran, Iran.

Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process. In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into chitosan-based nanofiber mats to fabricate a novel wound dressing. Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively. Read More

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December 2018

Characterization of Hydrocarbon-Degrading Bacteria in Constructed Wetland Microcosms Used to Treat Crude Oil Polluted Water.

Bull Environ Contam Toxicol 2018 Dec 12. Epub 2018 Dec 12.

Department of Bioscience, Aarhus University Centre for Water Technology (WATEC), Aarhus University, 8000, Århus C, Denmark.

Ten plant species were grown in constructed wetlands (CWs) to remediate water containing 2% (w/v) crude oil. The plant species with better growth and biomass production were Typha latifolia and Cyperus laevigatus, and they were significantly correlated (R = 0.91) with hydrocarbon degradation. Read More

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December 2018

Antimicrobial Activity and Cytotoxicity of Ag(I) and Au(I) Pillarplexes.

Front Chem 2018 27;6:584. Epub 2018 Nov 27.

Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway.

The biological activity of four pillarplex compounds featuring different metals and anions was investigated. The toxicity of the compounds against four bacterial strains [ (ATCC6633), (ATCC6538), (UVI isolate), ], one fungus (), and a human cell line (HepG2) was determined. Additionally, a UV-Vis titration study of the pillarplexes was carried out to check for stability depending on pH- and chloride concentration changes and evaluate the applicability in physiological media. Read More

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November 2018

Disarming the virulence arsenal of by blocking two-component system signaling.

Chem Sci 2018 Oct 10;9(37):7332-7337. Epub 2018 Jul 10.

Department of Chemistry , University of Minnesota , 225 Pleasant St. SE , Minneapolis , MN 55454 , USA . Email:

infections have reached a "critical" threat status making novel therapeutic approaches required. Inhibiting key signaling enzymes known as the histidine kinases (HKs), which are heavily involved with its pathogenicity, has been postulated to be an effective new strategy for treatment. Herein, we demonstrate the potential of this approach with benzothiazole-based HK inhibitors that perturb multiple virulence pathways in the burn wound isolate, PA14. Read More

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October 2018

Global proteomic profiling of infection by a giant phage.

J Virol 2018 Dec 12. Epub 2018 Dec 12.

Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, New York, USA

The 240-kb phage SPN3US genome encodes 264 gene products, many of which are functionally uncharacterized. We have previously used mass spectrometry to define the proteomes of wild-type and mutant forms of the SPN3US virion. In this study we sought to determine if this technique was suitable for the characterization of the SPN3US proteome during liquid infection. Read More

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December 2018

Antibiofilm elastin-like polypeptide coatings: functionality, stability, and selectivity.

Acta Biomater 2019 Jan 27;83:245-256. Epub 2018 Oct 27.

Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden. Electronic address:

Antimicrobial peptides (AMPs) are currently receiving interest as an alternative to conventional antibiotics to treat biomaterial-associated infection. However, the inherent instability of such peptides often limits their efficacy in intended clinical applications. Covalent immobilization of AMPs to surfaces is one strategy to increase the long-term stability and minimize the toxicity. Read More

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January 2019

Dual Transcriptomics of Host-Pathogen Interaction of Cystic Fibrosis Isolate PASS1 With Zebrafish.

Front Cell Infect Microbiol 2018 22;8:406. Epub 2018 Nov 22.

Department of Molecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW, Australia.

is a significant cause of mortality in patients with cystic fibrosis (CF). To explore the interaction of the CF isolate PASS1 with the innate immune response, we have used (zebrafish) as an infection model. Confocal laser scanning microscopy (CLSM) enabled visualization of direct interactions between zebrafish macrophages and PASS1. Read More

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November 2018

The Effects of Combined Abiotic and Pathogen Stress in Plants: Insights From Salinity and pv Interaction in Cucumber.

Front Plant Sci 2018 20;9:1691. Epub 2018 Nov 20.

Department of Plant Biotechnology and Cytogenetics, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, Poland.

Plants are often challenged by abiotic and biotic stresses acting in combination and the response to combinatorial stress differs from that triggered by each factor individually. Although salinity and pathogens are major stressors limiting plant growth and productivity worldwide, their interaction is poorly understood. The reactions to pathogens overlap with those to abiotic stresses, and reactive oxygen species (ROS) and stress hormones represent central nodes in the interacting signaling pathways. Read More

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November 2018

Potential Properties of Lactobacillus plantarum F-10 as a Bio-control Strategy for Wound Infections.

Probiotics Antimicrob Proteins 2018 Dec 6. Epub 2018 Dec 6.

Faculty of Science, Department of Biology, Ankara University, Tandogan, 06100, Ankara, Turkey.

In this study, Lactobacillus plantarum F-10, a promising probiotic strain isolated from fecal microbiota of healthy breastfed infant, was assessed as a bio-control strategy for wound infections. Pseudomonas aeruginosa PAO1/ATCC 27853, methicillin-resistant Staphylococcus aureus ATCC 43300, and their hospital-derived strains isolated from skin chronic wound samples were used as important skin pathogens. The cell-free extract (CFE) of the strain F-10 was shown to inhibit the growth of all pathogens tested, while no inhibition was observed when CFE was neutralized. Read More

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December 2018

Biological synthesis of high-conductive pili in aerobic bacterium Pseudomonas aeruginosa.

Appl Microbiol Biotechnol 2018 Dec 6. Epub 2018 Dec 6.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Bioelectrical nanowires as ecomaterials have great potential on environmental applications. A wide range of bacteria can express type IV pili (T4P), which are long protein fibers assembled from PilA. The T4P of Geobacter sulfurreducens are well known as "microbial nanowires," yet T4P of Pseudomonas aeruginosa (PaT4P) was believed to be poorly conductive. Read More

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December 2018

The selective pressures on the microbial community in a metal-contaminated aquifer.

ISME J 2018 Dec 6. Epub 2018 Dec 6.

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

In many environments, toxic compounds restrict which microorganisms persist. However, in complex mixtures of inhibitory compounds, it is challenging to determine which specific compounds cause changes in abundance and prevent some microorganisms from growing. We focused on a contaminated aquifer in Oak Ridge, Tennessee, USA that has large gradients of pH and widely varying concentrations of uranium, nitrate, and many other inorganic ions. Read More

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December 2018
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Outcomes of empiric aminoglycoside monotherapy for Pseudomonas aeruginosa bacteremia.

Diagn Microbiol Infect Dis 2018 Nov 9. Epub 2018 Nov 9.

Department of Pharmacy, Baylor St. Luke's Medical Center, 6720 Bertner Avenue, Houston, Texas 77030, USA; Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, 4849 Calhoun Road, Houston, Texas 77204, USA. Electronic address:

We evaluated 30-day mortality in patients with Pseudomonas aeruginosa bacteremia. There was no significant difference in mortality among patients who received functional aminoglycoside monotherapy versus inappropriate empiric therapy. Among patients given appropriate empiric therapy, functional aminoglycoside monotherapy was associated with less favorable outcomes compared to beta-lactam monotherapy. Read More

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November 2018

[Surveillance of resistance in the intensive care units using a cumulative antibiogram].

Mikrobiyol Bul 2018 Oct;52(4):329-339

Haydarpaşa Numune Training and Research Hospital, Department of Medical Microbiology, Istanbul, Turkey.

Empiric treatment with broad-spectrum antibiotics exerts condensed pressure in intensive care units (ICUs) for the selection of multidrug-resistant bacteria. Multidrug-resistant gram-negative bacteria became the focus of interest owing to limited treatment options and negative effects on patient survival. Cumulative antibiograms can guide selection of correct empiric treatment, de-escalation treatment according to antibiogram results and development of policies in fight against antibiotic resistance. Read More

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October 2018

Integrative network-centric approach reveals signaling pathways associated with plant resistance and susceptibility to Pseudomonas syringae.

PLoS Biol 2018 Dec 12;16(12):e2005956. Epub 2018 Dec 12.

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.

Plant protein kinases form redundant signaling pathways to perceive microbial pathogens and activate immunity. Bacterial pathogens repress cellular immune responses by secreting effectors, some of which bind and inhibit multiple host kinases. To understand how broadly bacterial effectors may bind protein kinases and the function of these kinase interactors, we first tested kinase-effector (K-E) interactions using the Pseudomonas syringae pv. Read More

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December 2018

Identification of molecular signatures of cystic fibrosis disease status using plasma-based functional genomics

Physiol Genomics 2018 12 12. Epub 2018 Dec 12.

Department of Pediatrics, Medical College of Wisconsin, United States.

Although cystic fibrosis (CF) is attributed to dysfunction of a single gene, the relationships between the abnormal gene product and development of inflammation and progression of lung disease are not fully understood, limiting our ability to predict an individual patient's clinical course and treatment response. To better understand CF progression, we characterized the molecular signatures of CF disease status using plasma-based functional genomics. Peripheral blood mononuclear cells (PBMCs) from healthy donors were cultured with plasma samples from CF patients (n=103) and unrelated, healthy controls (n=31). Read More

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December 2018
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Snow angels - the microbiology of freshly fallen snow: implications for immunocompromised patients.

J Water Health 2018 Dec;16(6):1029-1032

Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast, Northern Ireland, BT9 7AD, UK E-mail: School of Biomedical Sciences, Ulster University, Cromore Road, Coleraine, BT52 1SA, Northern Ireland, UK and Centre for Experimental Medicine, Queen's University, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.

The frequency of seasonal snowfall results in the transient covering of gardens/amenity sites/open public spaces, which encourages recreational interaction mainly with children. No data is available demonstrating the microbiological composition of such fallen snow and therefore a study was undertaken to examine the microbiology of snow from 37 sites, estimating (i) total viable count (TVC), (ii) identification of bacteria, and (iii) the presence of Pseudomonas aeruginosa. Mean TVC count of 8. Read More

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December 2018

Fast, economic and simultaneous identification of clinically relevant Gram-negative species with multiplex real-time PCR.

Future Microbiol 2018 Dec 12. Epub 2018 Dec 12.

Research & Development, Abbott (Alere Technologies GmbH), Jena, Germany.

Aim: A newly designed multiplex real-time PCR (rt-PCR) was validated to detect four clinically relevant Gram-negative bacteria (Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa).

Materials & Methods: Serial dilutions of genomic DNA were used to determine the limit of detection. Colony PCR was performed with isolates of the four selected species and other species as negative controls. Read More

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December 2018

Delafloxacin meglumine for the treatment of acute bacterial skin and skin structure infections (ABSSSI).

Drugs Today (Barc) 2018 Nov;54(11):657-666

Division of Microbiology, CSIR-Central Drug Research Institute, Lucknow, India.

Delafloxacin meglumine (Baxdela, WQ-3034, ABT-492, RX-3341; Melinta Therapeutics) was approved by the U.S. Food and Drug Administration (FDA) in June 2017 for the treatment of acute bacterial skin and skin structure infections on the basis of data from two phase III trials. Read More

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November 2018
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High-Temperature Short-Time Treatment of Human Milk for Bacterial Count Reduction.

Front Pediatr 2018 27;6:359. Epub 2018 Nov 27.

Center for Pediatrics, Department of Neonatology, Faculty of Medicine, Medical Center - University of Freiburg, Freiburg, Germany.

Human milk (HM) for preterm infants will often be pasteurized for cytomegalovirus (CMV) inactivation and reduction of its bacterial count. High-temperature short-time (HTST) treatment compared to standard Holder pasteurization (HoP) reduces the impact of heat treatment on bioactive HM proteins while effectively inactivating CMV. No data are available for the efficacy of bacterial count reduction using HTST treatments that are available for clinical use. Read More

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November 2018
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Interactions Between Immunotoxins and the Plasma Membrane: Implications for CAT-8015 Immunotoxin Therapy.

Front Oncol 2018 27;8:553. Epub 2018 Nov 27.

College of Engineering, Mathematics and Physical Sciences, Department of Physics and Astronomy, University of Exeter, Exeter, United Kingdom.

Acute Lymphoblastic Leukemia (ALL) remains the most frequent cause of cancer-related mortality in children and novel therapies are needed for the treatment of relapsed/refractory childhood ALL. One approach is the targeting of ALL blasts with the immunotoxin CAT-8015. Although CAT-8015 has potent anti-leukemia activity, with a 32% objective response rate in a phase 1 study of childhood ALL, haemolytic-uremic syndrome (HUS) and vascular leak syndrome (VLS), major dose-limiting toxicities, have limited the use of this therapeutic approach in children. Read More

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November 2018