12,259 results match your criteria Proteomics[Journal]


The olfactory bulb proteotype differs across frontotemporal dementia spectrum.

J Proteomics 2019 Apr 15. Epub 2019 Apr 15.

Clinical Neuroproteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea, 3, 31008 Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain, Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Mild olfactory dysfunction has been observed in frontotemporal dementias (FTD). However, the underlying molecular mechanisms associated to this deficit are poorly understood. We applied quantitative proteomics to analyze pathological effects on the olfactory bulb (OB) from progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration (FTLD-TDP43) subjects respect to elderly non-FTD group. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.011DOI Listing
April 2019
1 Read

Plasma proteomics and the paediatric patient.

Expert Rev Proteomics 2019 Apr 18. Epub 2019 Apr 18.

a Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne Australia.

Introduction: Plasma proteomics has been utilised extensively for studies that investigate various disease settings (e.g. cardiovascular disease), as well as to monitor the effect of pharmaceuticals on the plasma proteome (e. Read More

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http://dx.doi.org/10.1080/14789450.2019.1608186DOI Listing

Analysis of Litopenaeus vannamei hemocyanin interacting proteins reveals its role in in hemolymph clotting.

J Proteomics 2019 Apr 13. Epub 2019 Apr 13.

Department of Biology, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. Electronic address:

Hemocyanin is the main component of hemolymph plasma proteins and possesses diverse immunological properties and immunomodulatory functions. However, the interacting networks of hemocyanin in shrimp immune response remain poorly understood. In this study, 39 potential hemocyanin interacting partners were identified from Litopenaeus vannamei plasma by co-immunoprecipitation (Co-IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.013DOI Listing

Comparative proteomic analysis of saliva from dogs with and without obesity-related metabolic dysfuntion.

J Proteomics 2019 Apr 13. Epub 2019 Apr 13.

Interlab-UMU, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia 30100, Spain. Electronic address:

Dogs develop only some of the components of the human metabolic syndrome (MetS). Thus, in order to study possible MetS-related alterations in dogs, human MetS criteria were adapted to define canine MetS or so-called obesity-related metabolic dysfunction (ORMD). The main objective of this study was to identify changes in the salivary proteome of obese dogs with ORMD in comparison with obese dogs without ORMD which may constitute potential salivary biomarkers for assessing ORMD. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.010DOI Listing
April 2019
1 Read

Part II: Special Issue on Extracellular Vesicles and Exosomes.

Proteomics 2019 Apr;19(8):e1900121

Head, Molecular Proteomics, Baker Heart and Diabetes Institute, La Trobe Institute for Molecular Science, La Trobe University, Australia.

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http://dx.doi.org/10.1002/pmic.201900121DOI Listing

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Proteomics 2019 Apr;19(8):e1970064

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http://dx.doi.org/10.1002/pmic.201970064DOI Listing

Integrated Transcriptomic and Proteomic Analysis of Primary Human Umbilical Vein Endothelial cells.

Proteomics 2019 Apr 14:e1800315. Epub 2019 Apr 14.

A. K. Madugundu, A. Pandey, Center for Molecular Medicine, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bangalore, 560029, India.

Understanding the molecular profile of every human cell type is essential for understanding its role in normal physiology and disease. Technological advancements in DNA sequencing, mass spectrometry and computational methods allow us to carry out multi-Omics analyses although such approaches are not routine yet. Human umbilical vein endothelial cells (HUVECs) are a widely-used model system to study pathological and physiological processes associated with the cardiovascular system. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.2018003
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http://dx.doi.org/10.1002/pmic.201800315DOI Listing
April 2019
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Improved Methodology for Sensitive and Rapid Quantitative Proteomic Analysis of Adult-Derived Mouse Microglia: Application to a Novel In Vitro Mouse Microglial Cell Model.

Proteomics 2019 Apr 12:e1800469. Epub 2019 Apr 12.

J. Guergues, S. M. Stevens, Jr., Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Colchester, VT, USA.

Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain which can limit their relevance to homeostatic function and disease-related neuroimmune responses in the adult brain. Recently, an adult mouse brain-derived microglial cell line was established; however, a comprehensive proteome dataset remains lacking. Read More

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http://dx.doi.org/10.1002/pmic.201800469DOI Listing
April 2019
1 Read

Proteomic manifestations of genetic defects in autosomal recessive congenital ichthyosis.

J Proteomics 2019 Apr 9. Epub 2019 Apr 9.

Department of Environmental Toxicology, University of California, Davis, CA, United States of America. Electronic address:

Numerous genetic conditions give rise to a scaly skin phenotype as a result of impaired barrier function. Present work investigates the degree to which the departure from normal of ichthyosis corneocytes on the skin surface depends upon the basic defect as judged by proteomic profiling. Analyzing autosomal recessive congenital ichthyosis arising from defects in the genes PNPLA1, SDR9C7 and TGM1 revealed that profiles of PNPLA1 samples displayed the greatest degree of departure from normal control epidermis, with SDR9C7 samples nearly as divergent, and TGM1 the least divergent. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.007DOI Listing
April 2019
5 Reads

Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics.

J Proteomics 2019 Apr 9;201:1-11. Epub 2019 Apr 9.

Spanish National Research Council (CSIC), Marine Research Institute (IIM), Department of Food Technology, Vigo, Pontevedra, Spain. Electronic address:

Anisakis simplex is a parasitic nematode that can cause anisakiosis and/or allergic reactions in humans. The presence of invasive third-stage larvae (L3) in many different consumed fish species and the fourth-stage larvae (L4) in marine mammals, where L3 can accidentally affect to humans and develop as far as stage L4. World Health Organization and food safety authorities aim to control and prevent this emerging health problem. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.006DOI Listing
April 2019
7 Reads

Plasma metabolomics in early Alzheimer's disease patients diagnosed with amyloid biomarker.

J Proteomics 2019 Apr 9;200:144-152. Epub 2019 Apr 9.

Neonatal Research Unit, Health Research Institute La Fe, Valencia, Spain. Electronic address:

An untargeted metabolomics study has been carried out using plasma samples from patients with Mild Cognitive Impairment due to Alzheimer's disease patients (MCI-AD, n = 29) and healthy people (n = 29)). They have been classified following the National Institute on Aging and Alzheimer's Association (NIA-AA) recommendations and cerebrospinal fluid biomarkers. The analytical method was based on liquid chromatography coupled to high resolution mass spectrometry. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.008DOI Listing
April 2019
4 Reads

Identification, Quantification and Systems Analysis of Protein N-ε Lysine Methylation in Anucleate Blood Platelets.

Proteomics 2019 Apr 11:e1900001. Epub 2019 Apr 11.

Department of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, USA.

Protein post-translational modifications critically regulate a range of physiological and disease processes. In addition to tyrosine, serine and threonine phosphorylation, reversible N-ε acylation and alkylation of protein lysine residues also modulate diverse aspects of cellular function. Studies of lysine acyl and alkyl modifications have focused on nuclear proteins in epigenetic regulation; however, lysine modifications are also prevalent on cytosolic proteins to serve increasingly apparent, although less understood roles in cell regulation. Read More

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http://dx.doi.org/10.1002/pmic.201900001DOI Listing
April 2019
3 Reads

Molecular characterization of B-cell epitopes for the major fish allergen, parvalbumin, by shotgun proteomics, protein-based bioinformatics and IgE-reactive approaches.

J Proteomics 2019 Apr 8;200:123-133. Epub 2019 Apr 8.

Spanish National Research Council (CSIC), Marine Research Institute (IIM), Food Technology, Vigo, Pontevedra, Spain.

Parvalbumins beta (β-PRVBs) are the main fish allergens. The only proven and effective treatment for this type of hypersensitivity is to consume a diet free of fish. We present the molecular characterization of B-cell epitopes by shotgun proteomics of different β-PRVBs combined with protein-based bioinformatics and IgE-reactive approaches. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.005DOI Listing
April 2019
4 Reads

Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in adolescent, Normal-Weight Females.

Proteomics Clin Appl 2019 Apr 9:e1800184. Epub 2019 Apr 9.

Institute for Life Sciences, University of Southampton, Southampton, UK.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study was to examine the global serum proteomic profile of adolescent, normal-weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology but also identify novel circulating markers that can guide intervention protocols.

Methods: Non-depleted serum from normal-weight (BMI: 18-23 kg/m ), adolescent females (13 to 21 years old) with PCOS (n = 20) was compared to BMI- and age-matched healthy controls (n = 20) using our three-dimensional quantitative proteomics methodology. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.2018001
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http://dx.doi.org/10.1002/prca.201800184DOI Listing
April 2019
6 Reads

Proteomics Approaches to Define Senescence Heterogeneity and Chemotherapy Response.

Proteomics 2019 Apr 10:e1800447. Epub 2019 Apr 10.

C. Petit, J. Guillon, B. Toutain, A. Boissard, A. Patsouris, E. Lelièvre, C. Guette, O. Coqueret, Paul Papin ICO Cancer Center, CRCINA, INSERM, Université de Nantes, Université d'Angers, Angers, France.

In primary cells, senescence induces a permanent proliferative arrest to prevent the propagation of malignant cells. However, the outcome of senescence is more complex in advanced cancer cells where senescent states are heterogeneous. Here, we discuss this heterogeneity and propose that proteomic analysis should be used to identify specific signatures of cancer cells that use this pathway as an adaptative mechanism. Read More

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http://dx.doi.org/10.1002/pmic.201800447DOI Listing
April 2019
7 Reads

Downregulation of CENPF Remodels Prostate Cancer Cells and Alters Cellular Metabolism.

Proteomics 2019 Apr 8:e1900038. Epub 2019 Apr 8.

Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.

Metabolic alterations in prostate cancer (PC) are associated with progression and aggressiveness. However, the underlying mechanisms behind PC metabolic functions are unknown. Our group recently reported on the important role of centromere protein F (CENPF), a protein associated with the centromere-kinetochore complex and chromosomal segregation during mitosis, in PC MRI visibility. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.2019000
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http://dx.doi.org/10.1002/pmic.201900038DOI Listing
April 2019
4 Reads

iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low-temperature stress.

J Proteomics 2019 Apr 4. Epub 2019 Apr 4.

College of Life Sciences, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China. Electronic address:

Low temperatures profoundly influence the physiological and behavioural processes of ectotherms, especially teleosts, which have made them the subjects of strong interest over time. However, the characteristics of fish cold-tolerance at the protein level remain unclear. Therefore, to shed further light on the molecular mechanisms of low temperature adaptation in fish, we conducted quantitative proteomics on the T. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.004DOI Listing
April 2019
3 Reads
3.888 Impact Factor

Investigation of the estuarine stonefish (Synanceia horrida) venom composition.

J Proteomics 2019 Apr 3. Epub 2019 Apr 3.

Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland, 4072, Australia. Electronic address:

The Estuarine stonefish (Synanceia horrida) is recognised as one of the most venomous fish species in the world but the overall venom composition has yet to be investigated using in-depth transcriptomic and proteomic methods. To date, known venom components are restricted to a hyaluronidase and a large, pore-forming toxin known as Stonustoxin (SNTX). Transcriptomic sequencing of the venom gland resulted in over 170,000 contigs with only 0. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.002DOI Listing
April 2019
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Proteome profiling of carbapenem-resistant K. pneumoniae clinical isolate (NDM-4): Exploring the mechanism of resistance and potential drug targets.

J Proteomics 2019 Apr 3;200:102-110. Epub 2019 Apr 3.

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. Electronic address:

The emergence of carbapenem resistance has become a major problem worldwide. This has made treatment of K. pneumoniae infections a difficult task. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.003DOI Listing
April 2019
3 Reads
3.888 Impact Factor

Metabolic disorder of amino acids, fatty acids and purines reflects the decreases in oocyte quality and potential in sows.

J Proteomics 2019 Apr 3;200:134-143. Epub 2019 Apr 3.

State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, PR China; Beijing Biofeed Additive Key Laboratory, China Agricultural University, Beijing 100193, PR China.

Oocyte quality is closely related to female fertility. Nevertheless, core nutritional metabolites influencing oocyte quality are unclear. Herein, comprehensive metabolomics analysis of follicular fluid, serum, and urine from low reproductive performance (LRP) and normal reproductive performance (NRP) sows was conducted. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.015DOI Listing
April 2019
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Sex-specific differences in the intracellular proteome of human endothelial cells from dizygotic twins.

J Proteomics 2019 Apr 2. Epub 2019 Apr 2.

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, Berlin, Germany; DZHK (German Centre for Cardiovascular Research), partner site Berlin, Germany.

Differences between men and women are being continuously identified in many human diseases. The underlying reasons are not yet fully understood. Beside the influence of endogenous hormones and life style, intrinsic sex-specific dimorphisms at the cellular level may also play a role. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.016DOI Listing
April 2019
3 Reads

ANXA2 and FLNA phosphorylation associate with poor prognosis in hepatic carcinoma revealed by quantitative phosphoproteomics analysis.

J Proteomics 2019 Apr 2;200:111-122. Epub 2019 Apr 2.

The School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350004, People's Republic of China. Electronic address:

Hepatoma is one of the most common malignant tumors, and most patients have very poor prognosis. Early prediction and intervention of the hepatoma recurrence/metastasis are the most effective way to improve the patients' clinical outcomes. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative phospho-proteomics approach to identify biomarkers associated with hepatoma recurrence/metastasis in hepatoma cell lines with increasing metastasis ability. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.017DOI Listing
April 2019
3 Reads

iTRAQ-based quantitative proteomic analysis strengthens transcriptomic subtyping of triple-negative breast cancer tumors.

Proteomics 2019 Apr 5:e1800484. Epub 2019 Apr 5.

INSERM U1232, 44007, Nantes Cedex, France.

Heterogeneity and lack of targeted therapies represent the two main impediments to precision treatment of triple-negative breast cancer (TNBC). Therefore, molecular subtyping and identification of therapeutic pathways are required to optimize medical care. The aim of the present study was to define robust TNBC subtypes with clinical relevance by means of proteomics and transcriptomics. Read More

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http://dx.doi.org/10.1002/pmic.201800484DOI Listing
April 2019
2 Reads

iTRAQ-Based Quantitative Proteomic Analysis of Duck Eggshell During Biomineralization.

Proteomics 2019 Apr 5:e1900011. Epub 2019 Apr 5.

F. Zhu, F. Zhang, Z. -T. Yin, S. -R. Chen, N. Yang, Z. -C. Hou, National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.

The avian egg is a valuable model for the calcitic biomineralization process as it is the fastest calcification process occurring in nature and is a clear example of biomineralization. In this study, we used iTRAQ MS/MS to detect and study for the first time: 1) the overall duck eggshell proteome; 2) regional differences in the proteome between the inner and outer portions of the duck eggshell. The new reference protein datasets allowed us to identify 179 more eggshell proteins than solely using the current release of Ensembl duck annotations. Read More

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http://dx.doi.org/10.1002/pmic.201900011DOI Listing
April 2019
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Challenges and Opportunities in Cancer Metabolomics.

Proteomics 2019 Apr 5:e1900042. Epub 2019 Apr 5.

Center for Precision Medicine, Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA.

Challenges in metabolomics for a given spectrum of disease are more or less comparable, ranging from the accurate measurement of metabolite abundance, compound annotation, identification of unknown constituents, and interpretation of untargeted and analysis of high throughput targeted metabolomics data leading to the identification of biomarkers. However, metabolomics approaches in cancer studies specifically suffer from several additional challenges and require robust ways to sample the cells and tissues in order to tackle the constantly evolving cancer landscape. These constraints include, but are not limited to, discriminating the signals from given cell types and those that are cancer specific, discerning signals that are systemic and confounded, cell culture-based challenges associated with cell line identities and media standardizations, the need to look beyond Warburg effects, citrate cycle, lactate metabolism, and identifying and developing technologies to precisely and effectively sample and profile the heterogeneous tumor environment. Read More

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http://dx.doi.org/10.1002/pmic.201900042DOI Listing
April 2019
2 Reads
3.807 Impact Factor

Unique Protein Profiles of Extracellular Vesicles as Diagnostic Biomarkers for Early and Advanced Non-Small Cell Lung Cancer.

Proteomics 2019 Apr 5:e1800160. Epub 2019 Apr 5.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, P. R. China.

Extracellular vesicles (EVs) mediate intercellular communication via transferring proteins and other biological molecules and have been recently investigated as biomarkers of disease. Sensitive and specific biomarkers are required for lung cancer diagnosis and prognosis. The present study screened for abnormal EV proteins in non-small cell lung cancer (NSCLC) using a quantitative proteomics strategy involving LC-MS/MS to identify ideal biomarkers for NSCLC diagnosis. Read More

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http://dx.doi.org/10.1002/pmic.201800160DOI Listing
April 2019
8 Reads

Proteotyping pluripotency with mass spectrometry.

Expert Rev Proteomics 2019 Apr 5:1-10. Epub 2019 Apr 5.

a Proteomics Unit , Spanish National Cancer Research Centre (CNIO) , Madrid , Spain.

Introduction: Pluripotency emerges transiently during embryogenesis in two main forms with different developmental potential, termed naïve and primed states. Importantly, these pluripotent states can be recapitulated in vitro under specific culture conditions, representing a unique model to study the regulatory principles of development and cellular plasticity. Areas covered: A complex network of signaling pathways that senses intrinsic and extrinsic cues controls the fine balance between self-renewal and differentiation. Read More

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http://dx.doi.org/10.1080/14789450.2019.1604229DOI Listing
April 2019
4 Reads
2.896 Impact Factor

Standard abbreviations.

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Proteomics 2019 Apr;19(7):e1970054

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http://dx.doi.org/10.1002/pmic.201970054DOI Listing
April 2019
2 Reads

New insights into the phylogeographic distribution of the 3FTx/PLA venom dichotomy across genus Micrurus in South America.

J Proteomics 2019 Apr 1;200:90-101. Epub 2019 Apr 1.

Evolutionary and Translational Venomics Laboratory, CSIC, Valencia, Spain. Electronic address:

Micrurus is a monophyletic genus of venomous coral snakes of the family Elapidae. The ~80 recognized species within this genus are endemic to the Americas, and are distributed from southeastern United States to northern Argentina. Although relatively few bites are recorded due to their reclusive nature, semi-fossorial habits, and their occurrence in sparsely populated areas, coral snakes possess powerful venoms that target the cholinergic system and, if early treatment is missed, can cause neuromuscular paralysis, respiratory failure, and death by asphyxiation within hours of envenoming. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.014DOI Listing
April 2019
3 Reads

DeepFunc: A Deep Learning Framework for Accurate Prediction of Protein Functions from Protein Sequences and Interactions.

Proteomics 2019 Apr 2:e1900019. Epub 2019 Apr 2.

F. Zhang, H. Song, M. Zeng, Y. Li, M. Li, School of Computer Science and Engineering, Central South University, Changsha, 410083, P.R. China.

Annotation of protein functions plays an important role in understanding life at the molecular level. High throughput sequencing produces massive numbers of raw proteins sequences and only about 1% of them have been manually annotated with functions. Experimental annotations of functions are expensive, time-consuming and do not keep up with the rapid growth of the sequence numbers. Read More

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http://dx.doi.org/10.1002/pmic.201900019DOI Listing
April 2019
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Molecular insights into cancer drug resistance from a proteomics perspective.

Expert Rev Proteomics 2019 Mar 29:1-17. Epub 2019 Mar 29.

a West China School of Basic Medical Sciences & Forensic Medicine , Sichuan University , Chengdu , PR China.

Introduction: Resistance to chemotherapy and development of specific and effective molecular targeted therapies are major obstacles facing current cancer treatment. Comparative proteomic approaches have been employed for the discovery of putative biomarkers associated with cancer drug resistance and have yielded a number of candidate proteins, showing great promise for both novel drug target identification and personalized medicine for the treatment of drug-resistant cancer. Areas covered: Herein, we review the recent advances and challenges in proteomics studies on cancer drug resistance with an emphasis on biomarker discovery, as well as understanding the interconnectivity of proteins in disease-related signaling pathways. Read More

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http://dx.doi.org/10.1080/14789450.2019.1601561DOI Listing
March 2019
3 Reads

In-depth proteomic analysis of Boleophthalmus pectinirostris skin mucus.

J Proteomics 2019 Mar 25;200:74-89. Epub 2019 Mar 25.

Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan 316022, China. Electronic address:

Fish skin mucus serves as the first line of defence against pathogens and external stressors. The mudskipper Boleophthalmus pectinirostris inhabits intertidal mudflats containing abundant and diverse microbial populations; thus, the skin and mucus of B. pectinirostris are very important for immune defence. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.013DOI Listing
March 2019
3 Reads

Advances, challenges and tools in characterizing bacterial serine, threonine and tyrosine kinases and phosphorylation target sites.

Expert Rev Proteomics 2019 Mar 28:1-11. Epub 2019 Mar 28.

b Department of Animal and Food Sciences , University of Delaware , Newark , DE , USA.

Introduction: The cellular response to infection by bacterial pathogens involves a complex and highly regulated series of pathways that carry messages to various parts of the cell. These messages are transferred using post-translational modifications including phosphorylation by kinases. Understanding the host's signaling pathways is valuable in identifying potential treatment targets, but the bacterial signaling pathways and host-pathogen crosstalk are equally important to the development of therapeutics. Read More

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http://dx.doi.org/10.1080/14789450.2019.1601015DOI Listing
March 2019
1 Read

Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology.

Proteomics 2019 Mar 27:e1800370. Epub 2019 Mar 27.

Dept. of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL.

The use of cultured cells has been instrumental in studying biochemical, molecular, and cellular processes. The composition of serum that cells are maintained in can have a profound impact on important cellular checkpoints. We have analyzed cell growth and apoptosis in an estrogen receptor positive breast cancer cell line in the presence of serum that have been treated to remove steroids or lipids, as well-described in the literature. Read More

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http://dx.doi.org/10.1002/pmic.201800370DOI Listing
March 2019
1 Read

Multi-Omics Profiling for NF1 Target Discovery in Neurofibromin (NF1) Deficient Cells.

Proteomics 2019 Mar 25:e1800334. Epub 2019 Mar 25.

Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Loss of NF1 is an oncogenic driver. In efforts to define pathways responsible for the development of neurofibromas and other cancers, we evaluated transcriptomic and proteomic changes in a non-malignant NF1 null cell line. We created NF1 null HEK293 cells using CRISPR/Cas9 technology and compared them to parental cells that express neurofibromin. Read More

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http://dx.doi.org/10.1002/pmic.201800334DOI Listing
March 2019
2 Reads

RAId: Knowledge Integrated Proteomics Web Service with Accurate Statistical Significance Assignment.

Proteomics 2019 Mar 25:e1800367. Epub 2019 Mar 25.

National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD, 20894, USA.

Mass spectrometry based proteomics starts with identifications of peptides and proteins, which provide the bases for forming the next-level hypotheses whose "validations" are often employed for forming even higher level hypotheses and so forth. Scientifically meaningful conclusions are thus attainable only if the number of falsely identified peptides/proteins is accurately controlled. For this reason, we continue to develop RAId in the past decade. Read More

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http://dx.doi.org/10.1002/pmic.201800367DOI Listing
March 2019
3 Reads

Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins.

J Proteomics 2019 Mar 21;200:60-73. Epub 2019 Mar 21.

Center of the Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University, Rio Claro, SP 13500, Brazil. Electronic address:

The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins. Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.012DOI Listing
March 2019
1 Read

Label-free proteomic analysis of silkworm midgut infected by Bombyx mori nuclear polyhedrosis virus.

J Proteomics 2019 Mar 20;200:40-50. Epub 2019 Mar 20.

Institute of Sericulture and System Biology, Southwest University, Chongqing 400716, China.

Bombyx mori nuclear polyhedrosis virus (BmNPV) is the most damaging virus for the production of silkworm cocoons. Antivirus research continues to be an important aspect of the silkworm industry. Two-dimensional electrophoresis and mass spectrometry have been applied for analyzing the midgut proteome of BmNPV-infected silkworms. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.011DOI Listing
March 2019
2 Reads
3.888 Impact Factor

Analysis of liver proteome and identification of critical proteins affecting milk fat, protein and lactose metabolism in dariy cattle with iTRAQ.

Proteomics 2019 Mar 23:e1800387. Epub 2019 Mar 23.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, 100193, China.

In this study, we investigated the proteomes of liver tissues in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved with milk synthesis in dairy cattle. Based on iTRAQ analysis, we detected 3,252 proteins in the liver tissues (false discovery rate (FDR) ≤ 0.01). Read More

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http://dx.doi.org/10.1002/pmic.201800387DOI Listing
March 2019
4 Reads
3.807 Impact Factor

Combining Rapid Data Independent Acquisition and CRISPR Gene Deletion for Studying Potential Protein Functions: A Case of HMGN1.

Proteomics 2019 Mar 22:e1800438. Epub 2019 Mar 22.

Yale Cancer Biology Institute, Yale University, West Haven, CT, 06516, USA.

CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, we performed gene knockout of HMGN1 by CRISPR in cancer cells and studied the following proteomic regulation events. In particular, we utilized DIA mass spectrometry (DIA-MS) and reproducibly measured more than 6200 proteins (protein- FDR 1%) and more than 82,000 peptide precursors in the single MS shots of two hours. Read More

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http://dx.doi.org/10.1002/pmic.201800438DOI Listing
March 2019
1 Read

The Protein Phosphorylation Landscape of Mouse Spermatids During Spermiogenesis.

Proteomics 2019 Mar 22:e1900055. Epub 2019 Mar 22.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 210029, China.

The characteristic tadpole-shape of sperm is formed from round spermatids via spermiogenesis, a process which results in dramatic morphological changes in the final stage of spermatogenesis in the testis. Protein phosphorylation, as one of the most important post-translational modifications, can regulate spermiogenesis; however, the phosphorylation events taking place during this process have not been systematically analyzed. In order to better understand the role of phosphorylation in spermiogenesis, we performed large-scale phosphoproteome profiling using IMAC and TiO enrichment. Read More

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http://dx.doi.org/10.1002/pmic.201900055DOI Listing
March 2019
1 Read
3.807 Impact Factor

Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices.

J Proteomics 2019 Mar 17;200:1-10. Epub 2019 Mar 17.

Université Clermont Auvergne, INRA, VetAgro Sup, UMR Herbivores, Saint-Genès-Champanelle, France.

This study analyzed the effect of gender on the abundances of 20 protein biomarkers of tenderness and/or intramuscular fat content in five muscles: Longissimus thoracis, previously identified as biomarkers of tenderness and/or intramuscular Semimembranosus, Rectus abdominis, Triceps brachii and Semitendinosus, from cows and steers of the Protected Designation Origin Maine Anjou. The protein abundances were quantified using Reverse Phase Protein Array with specific validated antibodies. Among the 20 studied proteins, the abundance of 8 biomarkers involved in energetic metabolism, contraction and cellular stress, was different according to gender. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.03.010DOI Listing
March 2019
1 Read

Oncogenic and Non-Malignant Pancreatic Exosome Cargo Reveal Distinct Expression of Oncogenic and Prognostic Factors Involved in Tumor Invasion and Metastasis.

Proteomics 2019 Apr 9;19(8):e1800158. Epub 2019 Apr 9.

Metabolic Signaling, School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 6102, Perth, Western Australia, Australia.

Exosomes are small extracellular membrane vesicles important in intercellular communication, with their oncogenic cargo attributed to tumor progression and pre-metastatic niche formation. To gain an insight into key differences in oncogenic composition of exosomes, human non-malignant epithelial and pancreatic cancer cell models and purified and characterized resultant exosome populations are utilized. Proteomic analysis reveals the selective enrichment of known exosome markers and signaling proteins in comparison to parental cells. Read More

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http://dx.doi.org/10.1002/pmic.201800158DOI Listing
April 2019
2 Reads

Standard abbreviations.

Authors:

Proteomics 2019 Mar;19(6):e1970044

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http://dx.doi.org/10.1002/pmic.201970044DOI Listing
March 2019
1 Read

Discovery of a Novel Connecting Link between Renin-Angiotensin System and Cancer in Barrett's Esophagus by Proteomic Screening.

Proteomics Clin Appl 2019 Mar 19:e1900006. Epub 2019 Mar 19.

School of Life & Allied Health Sciences, The Glocal University, Saharanpur, 247121, Uttar Pradesh, India.

The renin-angiotensin system (RAS) plays a central role in the regulation of homeostasis and blood pressure. This involves an important enzyme called angiotensin-converting enzyme that leads to the conversion of angiotensin I into angiotensin II. RAS has been reported to show association with inflammation, and in sporadic studies, with cancer. Read More

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http://dx.doi.org/10.1002/prca.201900006DOI Listing
March 2019
1 Read

Proteomes of Leaf-Growing Zones in Rice Genotypes with Contrasting Drought Tolerance.

Proteomics 2019 Mar 19:e1800310. Epub 2019 Mar 19.

Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia.

Plants require a distinctive cohort of enzymes to coordinate cell division and expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence, proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) are investigated to provide insights into the effect of soil drying on gene expression relative to the drought-sensitive genotype Nipponbare. Read More

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http://dx.doi.org/10.1002/pmic.201800310DOI Listing
March 2019
1 Read

Comparative analysis of proteomic and metabolomic profiles of different species of Paris.

J Proteomics 2019 Mar 16;200:11-27. Epub 2019 Mar 16.

National Center of Biomedical Analysis, Beijing 100850, China.

An extract prepared from species of Paris is the most widely consumed herbal product in China. The genus Paris includes a variety of genotypes with different medicinal component contents but only two are defined as official sources. Closely related species have different medicinal properties because of differential expression of proteins and metabolites. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.003DOI Listing
March 2019
6 Reads

GPX4 at the Crossroads of Lipid Homeostasis and Ferroptosis.

Proteomics 2019 Mar 19:e1800311. Epub 2019 Mar 19.

Department of Biology, Stanford University, Room 423, 327 Campus Drive, Stanford, CA, 94305, USA.

Oxygen is necessary for aerobic metabolism but can also contribute to the harmful oxidation of lipids and other macromolecules. Oxidation of cholesterol and of phospholipids containing polyunsaturated fatty acyl chains can lead to lipid peroxidation, membrane damage and cell death. Lipid hydroperoxides are key intermediates in the process of lipid peroxidation. Read More

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http://dx.doi.org/10.1002/pmic.201800311DOI Listing
March 2019
1 Read

Quantitative, Label-Free Proteomics in the Symptomatic Niemann-Pick, Type C1 Mouse Model Using Standard Flow Liquid Chromatography and Thermal Focusing Electrospray Ionization.

Proteomics 2019 Mar 19:e1800432. Epub 2019 Mar 19.

Department of Chemistry, University of Illinois at Chicago, Chicago, IL, 60607, USA.

Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system. This abnormal accumulation results in a cascade of pathophysiological events including progressive, cerebellar neurodegeneration, among others. Read More

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http://dx.doi.org/10.1002/pmic.201800432DOI Listing
March 2019
2 Reads

Refining a pathogen's genome to know the enemy better for the winning battles.

Proteomics 2019 Mar 18:e1900005. Epub 2019 Mar 18.

Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology,, Daegu, Republic of Korea.

Clavibacter michiganensis, subsp. michiganens is a gram positive bacterial pathogen infecting tomato, resulting in the great losses of yield and quality worldwide. Despite its great impact on economics, we do not fully understand the virulence factors from its genome probably due to imperfect genome annotation. Read More

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http://dx.doi.org/10.1002/pmic.201900005DOI Listing
March 2019
2 Reads