629 results match your criteria Proteome Science [Journal]


Hepatic protein Carbonylation profiles induced by lipid accumulation and oxidative stress for investigating cellular response to non-alcoholic fatty liver disease in vitro.

Proteome Sci 2019 27;17. Epub 2019 Mar 27.

2Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400 Thailand.

Background: Non-alcoholic fatty liver disease (NAFLD) is caused by excessive accumulation of fat within the liver, leading to further severe conditions such as non-alcoholic steatohepatitis (NASH). Progression of healthy liver to steatosis and NASH is not yet fully understood in terms of process and response. Hepatic oxidative stress is believed to be one of the factors driving steatosis to NASH. Read More

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http://dx.doi.org/10.1186/s12953-019-0149-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438040PMC

Proteome modifications on tomato under extreme high light induced-stress.

Proteome Sci 2018 24;16:20. Epub 2018 Nov 24.

1Department of Bioresource Engineering, Macdonald Campus, McGill University, 21,111 Lakeshore Boulevard, Sainte-Anne-de-Bellevue, Quebec H9X 3V9 Canada.

Background: Abiotic stress reduces photosynthetic yield and plant growth, negatively impacting global crop production and is a major constraint faced by agriculture. However, the knowledge on the impact on plants under extremely high irradiance is limited. We present the first in-depth proteomics analysis of plants treated with a method developed by our research group to generate a light gradient using an extremely intense light. Read More

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http://dx.doi.org/10.1186/s12953-018-0148-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260845PMC
November 2018
3 Reads

Key factors identified by proteomic analysis in maize ( L.) seedlings' response to long-term exposure to different phosphate levels.

Proteome Sci 2018 20;16:19. Epub 2018 Nov 20.

1Maize Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-huai River Plain, Ministry of Agriculture, Jinan, 250100 China.

Background: Maize seedlings are constantly exposed to inorganic phosphate (Pi)-limited environments. To understand how maize cope with low Pi (LP) and high Pi (HP) conditions, physiological and global proteomic analysis of QXN233 genotype were performed under the long-term Pi starvation and supplementation.

Methods: We investigated the physiological response of QXN233 genotype to LP and HP conditions and detected the changes in ion fluxes by non-invasive micro-test technology and gene expression by quantitative real-time polymerase chain reaction. Read More

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http://dx.doi.org/10.1186/s12953-018-0147-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247739PMC
November 2018
14 Reads

Calcitonin gene-related peptide promotes proliferation and inhibits apoptosis in endothelial progenitor cells via inhibiting MAPK signaling.

Proteome Sci 2018 14;16:18. Epub 2018 Nov 14.

3Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou City, 510515 Guangdong Province China.

Background: Calcitonin gene-related peptide (CGRP) contributes to bone formation by stimulating bone marrow stromal cell (BMSC) proliferation and differentiation. However, the proliferative and apoptotic effects of CGRP on bone marrow-derived endothelial progenitor cells (EPCs) have not been investigated.

Methods: We tested the effects of CGRP on EPC proliferation and apoptosis by Cell Counting Kit-8, flow cytometry, and studied the effects of CGRP on the expression of proliferation- and apoptosis-related markers in EPCs and the underlying mitogen-activated protein kinase (MAPK) signalling pathway by quantitative polymerase chain reaction and western blotting. Read More

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https://proteomesci.biomedcentral.com/articles/10.1186/s1295
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http://dx.doi.org/10.1186/s12953-018-0146-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236989PMC
November 2018
11 Reads

Comparative proteomic analysis of response to high temperature stress.

Proteome Sci 2018 27;16:17. Epub 2018 Oct 27.

2Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211 China.

Background: belongs to green macroalgae and is the dominant species of green tide. It is distributed worldwide and is therefore subject to high-temperature stress during the growth process. However, the adaptation mechanisms of the response of to high temperatures have not been clearly investigated yet. Read More

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https://proteomesci.biomedcentral.com/articles/10.1186/s1295
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http://dx.doi.org/10.1186/s12953-018-0145-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204280PMC
October 2018
3 Reads

Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates.

Proteome Sci 2018 18;16:16. Epub 2018 Aug 18.

1Department of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5 Canada.

Background: The non-receptor tyrosine kinase, SRMS (Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) is a member of the BRK family kinases (BFKs) which represents an evolutionarily conserved relative of the Src family kinases (SFKs). Tyrosine kinases are known to regulate a number of cellular processes and pathways via phosphorylating substrate proteins directly and/or by partaking in signaling cross-talks leading to the indirect modulation of various signaling intermediates. In a previous study, we profiled the tyrosine-phosphoproteome of SRMS and identified multiple candidate substrates of the kinase. Read More

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https://proteomesci.biomedcentral.com/articles/10.1186/s1295
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http://dx.doi.org/10.1186/s12953-018-0143-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098843PMC
August 2018
5 Reads

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Proteome Sci 2018 7;16:15. Epub 2018 Aug 7.

Morsani College of Medicine, Department of Otolaryngology-HNS, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612 USA.

Background: The sensory epithelium of the inner ear converts the mechanical energy of sound to electro-chemical energy recognized by the central nervous system. This process is mediated by receptor cells known as hair cells that express proteins in a timely fashion with the onset of hearing.

Methods: The proteomes of 3, 14, and 30 day-old mice cochlear sensory epithelia were revealed, using label-free quantitative mass spectrometry (LTQ-Orbitrap). Read More

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http://dx.doi.org/10.1186/s12953-018-0144-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6091194PMC
August 2018
2 Reads

Comparative iTRAQ proteomics revealed proteins associated with horn development in yak.

Proteome Sci 2018 24;16:14. Epub 2018 Jul 24.

Key Laboratory for Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, 730050 People's Republic of China.

Background: The practice of dehorning yak raises animal safety concerns, which have been addressed by selective breeding to obtain genetically hornless yak. The POLLED locus in yak has been studied extensively; however, little is known regarding the proteins that regulate horn bud development.

Methods: A differential proteomic analysis was performed to compare the skin from the horn bud region of polled yak fetuses and the horn bud tissue of horned yak fetuses using isobaric tags for relative and absolute quantitation (iTRAQ) technology coupled with 2D LC-MS/MS. Read More

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http://dx.doi.org/10.1186/s12953-018-0141-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056918PMC
July 2018
2 Reads

In-depth proteomic analyses of (greenlip abalone) nacre and prismatic organic shell matrix.

Proteome Sci 2018 15;16:11. Epub 2018 Jun 15.

2Department of Geobiology, Georg-August University of Göttingen, Goldschmidstr. 3, 37077 Göttingen, Germany.

Background: The shells of various species have served as models of invertebrate biomineralization and physical shell properties for more than 20 years. A focus of this research has been the nacreous inner layer of the shell with its conspicuous arrangement of aragonite platelets, resembling in cross-section a brick-and-mortar wall. In comparison, the outer, less stable, calcitic prismatic layer has received much less attention. Read More

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http://dx.doi.org/10.1186/s12953-018-0139-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003135PMC
June 2018
7 Reads

iTRAQ-Based Proteomic Analysis reveals possible target-related proteins and signal networks in human osteoblasts overexpressing FGFR2.

Proteome Sci 2018 19;16:12. Epub 2018 Jun 19.

Department of Plastic and Reconstructive Surgery, Huashan Hospital, Fudan University School of Medicine, No. 12, Wu Lu Mu Qi Road (M), Shanghai, 200040 China.

Background: Fibroblast growth factor receptor 2 (FGFR2) play a vital role in skeletogenesis. However, the molecular mechanisms triggered by FGFR2 in osteoblasts are still not fully understood. In this study, proteomics and bioinformatics analysis were performed to investigate changes in the protein profiles regulated by FGFR2, with the goal of characterizing the molecular mechanisms of FGFR2 function in osteoblasts. Read More

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http://dx.doi.org/10.1186/s12953-018-0140-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011184PMC
June 2018
2 Reads

Correction to: Proteomic characterization of adrenal gland embryonic development reveals early initiation of steroid metabolism and reduction of the retinoic acid pathway.

Proteome Sci 2018 30;16:10. Epub 2018 May 30.

Department of Nephrology and Rheumatology, University Medical Center Goettingen, Georg-August University Goettingen, Robert-Koch-Strasse 40, D-37075 Goettingen, Germany.

[This corrects the article DOI: 10.1186/s12953-015-0063-8.]. Read More

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http://dx.doi.org/10.1186/s12953-018-0138-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977475PMC
May 2018
3 Reads

Proteomic analysis of buccal gland secretion from fasting and feeding lampreys ().

Proteome Sci 2018 22;16. Epub 2018 May 22.

1School of Life Sciences, Liaoning Normal University, Dalian, 116081 People's Republic of China.

Background: Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process. Read More

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http://dx.doi.org/10.1186/s12953-018-0137-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964706PMC
May 2018
8 Reads
1.730 Impact Factor

In vitro gentamicin exposure alters caveolae protein profile in cochlear spiral ligament pericytes.

Proteome Sci 2018 16;16. Epub 2018 Mar 16.

1Harvard T.H. Chan School of Public Health, Department of Environmental Health, MIPS Program, Boston, MA USA.

Background: The aminoglycoside antibiotic gentamicin is an ototoxic drug and has been used experimentally to investigate cochlear damage induced by noise.We have investigated the changes in the protein profile associated with caveolae in gentamicin treated and untreated spiral ligament (SL) pericytes, specialized cells in the blood labyrinth barrier of the inner ear microvasculature. Pericytes from various microvascular beds express caveolae, protein and cholesterol rich microdomains, which can undergo endocytosis and transcytosis to transport small molecules in and out the cells. Read More

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http://dx.doi.org/10.1186/s12953-018-0132-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938607PMC
March 2018
3 Reads

ITRAQ-based quantitative proteomic analysis of processed L. for reducing the intestinal toxicity.

Proteome Sci 2018 17;16. Epub 2018 Apr 17.

1College of Traditional Chinese Pharmacy, Beijing University of Chinese Medicine, North Third Ring Road, Number 11, Chaoyang District, Beijing, 100029 People's Republic of China.

Background: L., a Traditional Chinese medicine (TCM), is commonly used for the treatment of hydropsy, ascites, constipation, amenorrhea, and scabies. Semen Euphorbiae Pulveratum, which is another type of that is commonly used in TCM practice and is obtained by removing the oil from the seed that is called paozhi, has been known to ease diarrhea. Read More

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http://dx.doi.org/10.1186/s12953-018-0136-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905050PMC
April 2018
2 Reads

Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab ().

Proteome Sci 2018 15;16. Epub 2018 Mar 15.

1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306 China.

Background: The finite marine resources make it difficult for us to obtain enough fish oil (FO) used in aquatic feeds. Another sustainable ingredients should be found to substitute FO. The effects of replacing FO with vegetable oil have been studied in a variety of crustaceans, but most studies have focused on the phenotypic effects. Read More

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http://dx.doi.org/10.1186/s12953-018-0135-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856368PMC
March 2018
6 Reads

Profiling the interactome of protein kinase C ζ by proteomics and bioinformatics.

Proteome Sci 2018 26;16. Epub 2018 Feb 26.

1Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, 300070 China.

Background: Protein kinase C ζ (PKCζ), an isoform of the atypical protein kinase C, is a pivotal regulator in cancer. However, the molecular and cellular mechanisms whereby PKCζ regulates tumorigenesis and metastasis are still not fully understood. In this study, proteomics and bioinformatics analyses were performed to establish a protein-protein interaction (PPI) network associated with PKCζ, laying a stepping stone to further understand the diverse biological roles of PKCζ. Read More

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http://dx.doi.org/10.1186/s12953-018-0134-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828088PMC
February 2018
2 Reads

Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease.

Proteome Sci 2018 14;16. Epub 2018 Feb 14.

1Department of Nutritional Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH UK.

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. However, its molecular pathogenesis is incompletely characterized and clinical biomarkers remain scarce. The aims of these experiments were to identify and characterize liver protein alterations in an animal model of early, diet-related, liver injury and to assess novel candidate biomarkers in NAFLD patients. Read More

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http://dx.doi.org/10.1186/s12953-018-0131-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813374PMC
February 2018
24 Reads

Effect of ovarian cancer ascites on SKOV-3 cells proteome: new proteins associated with aggressive phenotype in epithelial ovarian cancer.

Proteome Sci 2018 13;16. Epub 2018 Feb 13.

1Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Ciudad de México, Mexico.

Background: Epithelial ovarian cancer is the second most lethal gynecological cancer worldwide. Ascites can be found in all clinical stages, however in advanced disease stages IIIC and IV it is more frequent and could be massive, associated with worse prognosis. Due to the above, it was our interest to understanding how the ascites of ovarian cancer patients induces the mechanisms by which the cells present in it acquire a more aggressive phenotype and to know new proteins associated to this process. Read More

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http://dx.doi.org/10.1186/s12953-018-0133-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810116PMC
February 2018
6 Reads

Proteomic identification of moesin upon exposure to acrolein.

Proteome Sci 2018 17;16. Epub 2018 Jan 17.

1Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Wonmi-gu, Bucheon, Gyeonggi-do 420-767 South Korea.

Background: Acrolein (allyl Aldehyde) as one of smoke irritant exacerbates chronic airway diseases and increased in sputum of patients with asthma and chronic obstructive lung disease. But underlying mechanism remains unresolved. The aim of study was to identify protein expression in human lung microvascular endothelial cells (HMVEC-L) exposed to acrolein. Read More

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http://dx.doi.org/10.1186/s12953-017-0130-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773073PMC
January 2018
6 Reads

Chemical proteomics, an integrated research engine for exploring drug-target-phenotype interactions.

Proteome Sci 2018 9;16. Epub 2018 Jan 9.

Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109 Australia.

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http://dx.doi.org/10.1186/s12953-017-0129-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759357PMC
January 2018
2 Reads

Comparative proteomic analysis between nitrogen supplemented and starved conditions in .

Proteome Sci 2017 13;15:20. Epub 2017 Nov 13.

Center for Integrated Fungal Research, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695 USA.

Background: Fungi are constantly exposed to nitrogen limiting environments, and thus the efficient regulation of nitrogen metabolism is essential for their survival, growth, development and pathogenicity. To understand how the rice blast pathogen copes with limited nitrogen availability, a global proteome analysis under nitrogen supplemented and nitrogen starved conditions was completed.

Methods: strain 70-15 was cultivated in liquid minimal media and transferred to media with nitrate or without a nitrogen source. Read More

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http://dx.doi.org/10.1186/s12953-017-0128-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684745PMC
November 2017
5 Reads

Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots.

Proteome Sci 2017 30;15:19. Epub 2017 Oct 30.

Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural Science, Yaoyuan 1, Hongshan, Wuhan, Hubei 430017 China.

Background: Alfalfa () is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue variations in response to salt stress, comparative physiological and proteomic analyses were made of salt responses in the roots and shoots of the alfalfa.

Method: A two-dimensional gel electrophoresis (2-DE)-based proteomic technique was employed to identify the differentially abundant proteins (DAPs) from salt-treated alfalfa roots and shoots of the salt tolerance cultivars Zhongmu No 1 cultivar, which was subjected to a range of salt stress concentrations for 9 days. Read More

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http://dx.doi.org/10.1186/s12953-017-0127-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663070PMC
October 2017
7 Reads

Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes.

Proteome Sci 2017 4;15:18. Epub 2017 Aug 4.

Department of Epidemiology, Preclinical Research and Advanced Diagnostics, National Institute for Infectious Diseases L. Spallanzani, IRCCS, via Portuense 292, 00149 Rome, Italy.

Background: Changes in iron metabolism frequently accompany HIV-1 infection. However, while many clinical and in vitro studies report iron overload exacerbates the development of infection, many others have found no correlation. Therefore, the multi-faceted role of iron in HIV-1 infection remains enigmatic. Read More

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http://dx.doi.org/10.1186/s12953-017-0126-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545036PMC
August 2017
37 Reads

Examining the influence of specificity ligands and ATP-competitive ligands on the overall effectiveness of bivalent kinase inhibitors.

Proteome Sci 2016 17;15:17. Epub 2017 Jul 17.

Novartis Institutes for BioMedical Research, Cambridge, MA 02139 USA.

Background: Identifying selective kinase inhibitors remains a major challenge. The design of bivalent inhibitors provides a rational strategy for accessing potent and selective inhibitors. While bivalent kinase inhibitors have been successfully designed, no comprehensive assessment of affinity and selectivity for a series of bivalent inhibitors has been performed. Read More

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http://dx.doi.org/10.1186/s12953-017-0125-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513037PMC
July 2017
9 Reads

Daptomycin, a last-resort antibiotic, binds ribosomal protein S19 in humans.

Proteome Sci 2016 1;15:16. Epub 2017 Jul 1.

Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109 Australia.

Background: Daptomycin is a recently introduced, last-resort antibiotic that displays a unique mode of action against Gram-positive bacteria that is not fully understood. Several bacterial targets have been proposed but no human binding partner is known.

Methods: In the present study we tested daptomycin in cell viability and proliferation assays against six human cell lines, describe the synthesis of biotinylated and fluorescently labeled analogues of daptomycin. Read More

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http://dx.doi.org/10.1186/s12953-017-0124-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494143PMC
July 2017
5 Reads

Tetrazine ligation for chemical proteomics.

Proteome Sci 2016 26;15:15. Epub 2017 Jun 26.

Department of Molecular Science and Technology, Ajou University, Suwon, 16499 Republic of Korea.

Determining small molecule-target protein interaction is essential for the chemical proteomics. One of the most important keys to explore biological system in chemical proteomics field is finding first-class molecular tools. Chemical probes can provide great spatiotemporal control to elucidate biological functions of proteins as well as for interrogating biological pathways. Read More

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http://dx.doi.org/10.1186/s12953-017-0121-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485739PMC
June 2017
7 Reads

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs).

Proteome Sci 2016 24;15:14. Epub 2017 Jun 24.

Molecular Recognition Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seoul, 136-791 Republic of Korea.

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Read More

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http://dx.doi.org/10.1186/s12953-017-0123-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483283PMC
June 2017
23 Reads

Thermal proteome profiling: unbiased assessment of protein state through heat-induced stability changes.

Proteome Sci 2016 24;15:13. Epub 2017 Jun 24.

European Molecular Biology Laboratory, Genome Biology Unit, Meyerhofstr. 1, 69117 Heidelberg, Germany.

In recent years, phenotypic-based screens have become increasingly popular in drug discovery. A major challenge of this approach is that it does not provide information about the mechanism of action of the hits. This has led to the development of multiple strategies for target deconvolution. Read More

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http://dx.doi.org/10.1186/s12953-017-0122-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482948PMC
June 2017
2 Reads

The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment.

Proteome Sci 2016 20;15:12. Epub 2017 Jun 20.

Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061 USA.

Background: Liver models that closely mimic the in vivo microenvironment are useful for understanding liver functions, capabilities, and intercellular communication processes. Three-dimensional (3D) liver models assembled using hepatocytes and liver sinusoidal endothelial cells (LSECs) separated by a polyelectrolyte multilayer (PEM) provide a functional system while also permitting isolation of individual cell types for proteomic analyses.

Methods: To better understand the mechanisms and processes that underlie liver model function, hepatocytes were maintained as monolayers and 3D PEM-based formats in the presence or absence of primary LSECs. Read More

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http://proteomesci.biomedcentral.com/articles/10.1186/s12953
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http://dx.doi.org/10.1186/s12953-017-0120-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480101PMC
June 2017
36 Reads

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum.

Proteome Sci 2016 10;15:11. Epub 2017 Jun 10.

Proteomics and Small Molecule Mass Spectrometry, Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.

Background: Unlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive method to characterise the circulating acellular proteome in ovine serum.

Methods: Serum samples from healthy sheep were subjected to shotgun proteomic analysis using nano liquid chromatography nano electrospray ionisation tandem mass spectrometry (nanoLC-nanoESI-MS/MS) on a quadrupole time-of-flight instrument (TripleTOF® 5600+, SCIEX). Read More

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http://dx.doi.org/10.1186/s12953-017-0119-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466729PMC
June 2017
15 Reads

The effect of microbial challenge on the intestinal proteome of broiler chickens.

Proteome Sci 2016 30;15:10. Epub 2017 May 30.

Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, Glasgow University, Glasgow, UK.

Background: In poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. Uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. The exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. Read More

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http://dx.doi.org/10.1186/s12953-017-0118-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450085PMC
May 2017
6 Reads

Water deficit mechanisms in perennial shrubs leaves revealed by physiological and proteomic analyses.

Proteome Sci 2016 8;15. Epub 2017 May 8.

Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, 150040 People's Republic of China.

Background: Drought (Water deficit, WD) poses a serious threat to extensively economic losses of trees throughout the world. Chinese dwarf cherry () is a good perennial plant for studying the physiological and sophisticated molecular network under WD. The aim of this study is to identify the effect of WD on through physiological and global proteomics analysis and improve understanding of the WD resistance of plants. Read More

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http://proteomesci.biomedcentral.com/articles/10.1186/s12953
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http://dx.doi.org/10.1186/s12953-017-0117-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422899PMC
May 2017
36 Reads

Merging clinical chemistry biomarker data with a COPD database - building a clinical infrastructure for proteomic studies.

Proteome Sci 2016 21;15. Epub 2017 Apr 21.

Centre of Excellence in Biological and Medical Mass Spectrometry, Biomedical Centre D13, Lund University, 221 84 Lund, Sweden.

Background: Data from biological samples and medical evaluations plays an essential part in clinical decision making. This data is equally important in clinical studies and it is critical to have an infrastructure that ensures that its quality is preserved throughout its entire lifetime. We are running a 5-year longitudinal clinical study, KOL-Örestad, with the objective to identify new COPD (Chronic Obstructive Pulmonary Disease) biomarkers in blood. Read More

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http://dx.doi.org/10.1186/s12953-017-0116-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401459PMC
April 2017
14 Reads

Proteomic profiling of HBV infected liver biopsies with different fibrotic stages.

Proteome Sci 2016 20;15. Epub 2017 Apr 20.

Molecular Biology Biotechnology and Genetics Research Center (MOBGAM), Istanbul Technical University, Sariyer, Istanbul, Turkey.

Background: Hepatitis B virus (HBV) is a global health problem, and infected patients if left untreated may develop cirrhosis and eventually hepatocellular carcinoma. This study aims to enlighten pathways associated with HBV related liver fibrosis for delineation of potential new therapeutic targets and biomarkers.

Methods: Tissue samples from 47 HBV infected patients with different fibrotic stages (F1 to F6) were enrolled for 2D-DIGE proteomic screening. Read More

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http://dx.doi.org/10.1186/s12953-017-0114-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399407PMC
April 2017
11 Reads

Disulfide proteomics of rice cultured cells in response to OsRacl and probenazole-related immune signaling pathway in rice.

Proteome Sci 2016 13;15. Epub 2017 Apr 13.

Keio University, Institute for Advanced Biosciences, 246-2 Mizukami, Kakuganji, Tsuruoka, Yamagata 997-0052 Japan.

Background: Reactive oxygen species (ROS) production is an early event in the immune response of plants. ROS production affects the redox-based modification of cysteine residues in redox proteins, which contribute to protein functions such as enzymatic activity, protein-protein interactions, oligomerization, and intracellular localization. Thus, the sensitivity of cysteine residues to changes in the cellular redox status is critical to the immune response of plants. Read More

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http://dx.doi.org/10.1186/s12953-017-0115-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390479PMC
April 2017
15 Reads

Proteomic analysis of microparticles isolated from malaria positive blood samples.

Proteome Sci 2016 24;15. Epub 2017 Mar 24.

Noguchi Memorial Institute of Medical Research, University of Ghana, Legon, Ghana.

Background: Malaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma membrane derived extracellular vesicles (PMEVs) are subcellular structures that are generated when they bud off the plasma membrane. They can be found in healthy individuals but the numbers tend to increase in pathological conditions including malaria. Read More

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http://dx.doi.org/10.1186/s12953-017-0113-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366142PMC
March 2017
16 Reads
1.725 Impact Factor

Protein profiles of hatchery egg shell membrane.

Proteome Sci 2016 3;15. Epub 2017 Mar 3.

Statewide Mass Spectrometry Facility, University of Arkansas, Fayetteville, AR 72701 USA.

Background: Eggshells which consist largely of calcareous outer shell and shell membranes, constitute a significant part of poultry hatchery waste. The shell membranes (ESM) not only contain proteins that originate from egg whites but also from the developing embryos and different contaminants of microbial and environmental origins. As feed supplements, during post hatch growth, the hatchery egg shell membranes (HESM) have shown potential for imparting resistance of chickens to endotoxin stress and exert positive health effects. Read More

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http://dx.doi.org/10.1186/s12953-017-0112-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335749PMC
March 2017
2 Reads

Overgrazing induces alterations in the hepatic proteome of sheep (): an iTRAQ-based quantitative proteomic analysis.

Proteome Sci 2016 5;15. Epub 2017 Jan 5.

Key Laboratory of Forage Grass, Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, 010010 Inner Mongolia China.

Background: The degradation of the steppe of Inner Mongolia, due to overgrazing, has resulted in ecosystem damage as well as extensive reductions in sheep production. The growth performance of sheep is greatly reduced because of overgrazing, which triggers massive economic losses every year. The liver is an essential organ that has very important roles in multiple functions, such as nutrient metabolism, immunity and others, which are closely related to animal growth. Read More

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http://dx.doi.org/10.1186/s12953-016-0111-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267464PMC
January 2017
10 Reads

An interactomics overview of the human and bovine milk proteome over lactation.

Proteome Sci 2016 5;15. Epub 2017 Jan 5.

Dairy Science and Technology, Food Quality and Design Group, Wageningen University, Postbox 8129, 6700EV Wageningen, The Netherlands.

Background: Milk is the most important food for growth and development of the neonate, because of its nutrient composition and presence of many bioactive proteins. Differences between human and bovine milk in low abundant proteins have not been extensively studied. To better understand the differences between human and bovine milk, the qualitative and quantitative differences in the milk proteome as well as their changes over lactation were compared using both label-free and labelled proteomics techniques. Read More

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http://dx.doi.org/10.1186/s12953-016-0110-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5267443PMC
January 2017
3 Reads

Proteome changes in the small intestinal mucosa of growing pigs with dietary supplementation of non-starch polysaccharide enzymes.

Proteome Sci 2016 10;15. Epub 2017 Jan 10.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193 People's Republic of China.

Background: Non-starch polysaccharide enzymes (NSPEs) have long been used in monogastric animal feed production to degrade non-starch polysaccharides (NSPs) to oligosaccharides in order to promote growth performance and gastrointestinal (GI) tract health. However, the precise molecular mechanism of NSPEs in the improvement of the mammalian small intestine remains unknown.

Methods: In this study, isobaric tags were applied to investigate alterations of the small intestinal mucosa proteome of growing pigs after 50 days of supplementation with 0. Read More

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http://dx.doi.org/10.1186/s12953-016-0109-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223414PMC
January 2017
4 Reads

Informed baseline subtraction of proteomic mass spectrometry data aided by a novel sliding window algorithm.

Proteome Sci 2016 7;14:19. Epub 2016 Dec 7.

School of Mathematical Sciences, The University of Adelaide, North Terrace, Adelaide, 5005 Australia.

Background: Proteomic matrix-assisted laser desorption/ionisation (MALDI) linear time-of-flight (TOF) mass spectrometry (MS) may be used to produce protein profiles from biological samples with the aim of discovering biomarkers for disease. However, the raw protein profiles suffer from several sources of bias or systematic variation which need to be removed via pre-processing before meaningful downstream analysis of the data can be undertaken. Baseline subtraction, an early pre-processing step that removes the non-peptide signal from the spectra, is complicated by the following: (i) each spectrum has, on average, wider peaks for peptides with higher mass-to-charge ratios (/), and (ii) the time-consuming and error-prone trial-and-error process for optimising the baseline subtraction input arguments. Read More

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http://dx.doi.org/10.1186/s12953-016-0107-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142289PMC
December 2016
12 Reads

Follicular fluid biomarkers for human in vitro fertilization outcome: Proof of principle.

Proteome Sci 2016 14;14:17. Epub 2016 Nov 14.

Facility for Systems Biology based Mass Spectrometry (SYBIOMA), KU Leuven, Leuven, Belgium.

Background: Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported. Read More

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http://dx.doi.org/10.1186/s12953-016-0106-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109724PMC
November 2016
8 Reads

Proteomic based approach for characterizing 4-hydroxy-2-nonenal induced oxidation of buffalo () and goat () meat myoglobins.

Proteome Sci 2016 16;14:18. Epub 2016 Nov 16.

Proteomics Lab, National Centre for Cell Science, Pune, 411007 India.

Background: Myoglobin (Mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (HNE) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of Mb and predispose it to greater oxidation. However, no literature is available on characterization of lipid oxidation induced oxidation of Indian water buffalo () and goat () myoglobins. Read More

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http://dx.doi.org/10.1186/s12953-016-0108-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112711PMC
November 2016
11 Reads

iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels ().

Proteome Sci 2016 8;14:16. Epub 2016 Nov 8.

Key Laboratory of Resource Biology and Biotechnology in Western China (College of Life Sciences, Northwest University), Ministry of Education, Xi'an, 710069 People's Republic of China.

Background: Daurian ground squirrels () deviate from significant increase of protein catabolism and loss of myofibrillar contents during long period of hibernation inactivity.

Methods: Here we use iTRAQ based quantitative analysis to examine proteomic changes in the soleus of squirrels in pre-hibernation, hibernation and post-hibernation states. The total proteolysis rate of soleus was measured by the release of the essential amino acid tyrosine from isolated muscles. Read More

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http://dx.doi.org/10.1186/s12953-016-0105-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101720PMC
November 2016
2 Reads

Multi-omics analysis on the pathogenicity of ENHKU01 isolated from sewage outfalls along the Ningbo coastline.

Proteome Sci 2016 18;14:15. Epub 2016 Oct 18.

School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.

Background: The acquisition of iron is important for the pathogenicity of bacteria and blood. Three different culture environments (Fe stimulation, blood agar plate and normal plate) were used to stimulate , and their respective pathogenicities were compared at the proteomic, mRNA and metabolomic levels.

Methods: 2D-DIGE combined with MALDI-TOF-MS/MS, RT-PCR and H NMR were used to analyze the differential expression levels of proteins, mRNA and metabolites. Read More

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http://proteomesci.biomedcentral.com/articles/10.1186/s12953
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http://dx.doi.org/10.1186/s12953-016-0104-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070189PMC
October 2016
6 Reads

Proteome profiling reveals insights into cold-tolerant growth in sea buckthorn.

Proteome Sci 2016 7;14:14. Epub 2016 Oct 7.

Experimental Center of Desert Forestry, Chinese Academy of Forestry, InnerMonglia, People's Republic of China.

Background: Low temperature is one of the crucial environmental factors limiting the productivity and distribution of plants. Sea buckthorn ( L.), a well recognized multipurpose plant species, live successfully in in cold desert regions. Read More

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http://dx.doi.org/10.1186/s12953-016-0103-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054542PMC
October 2016
7 Reads

Comparative proteomic analyses of Asian cotton ovules with attached fibers in the early stages of fiber elongation process.

Proteome Sci 2016 8;14(1):13. Epub 2016 Sep 8.

Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, 100084 People's Republic of China.

Background: Plenty of proteomic studies were performed to characterize the allotetraploid upland cotton fiber elongation process, whereas little is known about the elongating diploid cotton fiber proteome.

Methods: In this study, we used a two-dimensional electrophoresis-based comparative proteomic approach to profile dynamic proteomes of diploid Asian cotton ovules with attached fibers in the early stages of fiber elongation process. One-way ANOVA and Student-Newman-Keuls test were used to find the differentially displayed protein (DDP) spots. Read More

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http://proteomesci.biomedcentral.com/articles/10.1186/s12953
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http://dx.doi.org/10.1186/s12953-016-0101-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015342PMC
September 2016
8 Reads

Identifying EGFR mutation-induced drug resistance based on alpha shape model analysis of the dynamics.

Proteome Sci 2016 8;14(1):12. Epub 2016 Sep 8.

Department of Electronic Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong.

Background: Epidermal growth factor receptor (EGFR) mutation-induced drug resistance is a difficult problem in lung cancer treatment. Studying the molecular mechanisms of drug resistance can help to develop corresponding treatment strategies and benefit new drug design.

Methods: In this study, Rosetta was employed to model the EGFR mutant structures. Read More

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http://dx.doi.org/10.1186/s12953-016-0102-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015241PMC
September 2016
3 Reads

Removal of SDS from biological protein digests for proteomic analysis by mass spectrometry.

Proteome Sci 2016 6;14(1):11. Epub 2016 Sep 6.

Department of Medicinal Crop Research, Rural Development Administration, Eumseong, Chungbuk, 369-873 Republic of Korea.

Background: Metal-organic frameworks (MOFs - MIL-101) are the most exciting, high profiled developments in nanotechnology in the last ten years, and it attracted considerable attention owing to their uniform nanoporosity, large surface area, outer-surface modification and in-pore functionality for tailoring the chemical properties of the material for anchoring specific guest moieties. MOF's have been particularly highlighted for their excellent gas storage and separation properties. Recently biomolecules-based MOF's were used as nanoencapsulators for antitumor and antiretroviral controlled drug delivery studies. Read More

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http://dx.doi.org/10.1186/s12953-016-0098-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012027PMC
September 2016
11 Reads
1.730 Impact Factor