6,749,855 results match your criteria Protein science : a publication of the Protein Society[Journal]

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Potential Role of microRNA-183 as a Tumor Suppressor in Hepatocellular Carcinoma.

Cell Physiol Biochem 2018 Dec 6;51(5):2065-2072. Epub 2018 Dec 6.

Department of General Surgery of East District, The Second Hospital of Hebei Medical University, Shijiazhuang,

Background/aims: Disseminated tumors, known as metastases, are responsible for ninety-percent of mortality due to cancer. Epithelial to mesenchymal transition, a phenomenon required for morphological conversion of non-motile discoid shaped epithelial cells to highly motile spindle-shaped mesenchymal cells, is thought to be a pre-requisite for metastatic progression. Metastasis-associated 1 (MTA1) protein is a prime inducer of EMT and metastatic progression in all solid tumors including hepatocellular carcinoma (HCC). Read More

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December 2018
8 Reads

Gliquidone Alleviates Diabetic Nephropathy by Inhibiting Notch/Snail Signaling Pathway.

Cell Physiol Biochem 2018 Dec 6;51(5):2085-2097. Epub 2018 Dec 6.

Background/aims: Diabetic nephropathy is a common complication of diabetes. This study explored the renal protective effect and possible mechanism of gliquidone in mice with diabetic nephropathy.

Methods: Animal model of diabetic nephropathy was established in KKAy mice. Read More

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December 2018
3 Reads

Gastric Adenocarcinoma Predictive Long Intergenic Non-Coding RNA Promotes Tumor Occurrence and Progression in Non-Small Cell Lung Cancer via Regulation of the miR-661/eEF2K Signaling Pathway.

Cell Physiol Biochem 2018 Dec 6;51(5):2136-2147. Epub 2018 Dec 6.

Background/aims: Long non-coding RNAs (lncRNAs) play vital roles in carcinogenesis as oncogenes or tumor suppressor genes. This study explored the biological function of lncRNA gastric adenocarcinoma predictive long intergenic non-coding RNA (GAPLINC) in human non-small cell lung cancer (NSCLC).

Methods: GAPLINC expression in NSCLC specimens and cell lines was detected by qRT-PCR and Western blot. Read More

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December 2018
2 Reads

miR-122 Targets X-Linked Inhibitor of Apoptosis Protein to Sensitize Oxaliplatin-Resistant Colorectal Cancer Cells to Oxaliplatin-Mediated Cytotoxicity.

Cell Physiol Biochem 2018 Dec 6;51(5):2148-2159. Epub 2018 Dec 6.

Minimally Invasive Treatment Center, Fudan University Shanghai Cancer Center, Shanghai,

Background/aims: Although oxaliplatin is one of the most effective chemotherapeutic drugs used to treat colorectal cancer (CRC), long-term administration usually induces acquired drug resistance during the course of treatment. Thus, there is an urgent need to explore novel strategies to improve the efficiency of cancer therapy. The aim of this study was to explore the effect of microRNA-122 (miR-122) on reversing oxaliplatin resistance in CRC. Read More

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December 2018
3 Reads

Blocking TRPA1 and TNF-α Signal Improves Bortezomib-Induced Neuropathic Pain.

Cell Physiol Biochem 2018 Dec 6;51(5):2098-2110. Epub 2018 Dec 6.

Background/aims: Bortezomib (BTZ) is largely used as a chemotherapeutic agent for the treatment of multiple myeloma. However, one of the significant limiting complications of BTZ is painful peripheral neuropathy during BTZ therapy. The purpose of this study was to examine the underlying mechanisms leading to neuropathic pain induced by BTZ. Read More

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December 2018
1 Read

Sestrin 2 Attenuates Rat Hepatic Stellate Cell (HSC) Activation and Liver Fibrosis via an mTOR/AMPK-Dependent Mechanism.

Cell Physiol Biochem 2018 Dec 6;51(5):2111-2122. Epub 2018 Dec 6.

Department of Infectious Diseases, The Third Affiliated Hospital of Wenzhou Medical University, Rui'an,

Background/aims: Sestrin 2 is associated with the pathophysiology of several diseases. The aim of this study was to investigate the effects and potential mechanisms of Sestrin 2 in rat hepatic stellate cells (HSCs) during liver fibrogenesis.

Methods: In this study, Sestrin 2 protein expression was detected in rat HSC-T6 cells challenged with transforming growth factor-β (TGF-β) and in mice treated with carbon tetrachloride (CCl4), a well-known model of hepatic fibrosis. Read More

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December 2018
2 Reads

miR-202 Enhances the Anti-Tumor Effect of Cisplatin on Non-Small Cell Lung Cancer by Targeting the Ras/MAPK Pathway.

Cell Physiol Biochem 2018 Dec 6;51(5):2160-2171. Epub 2018 Dec 6.

Background/aims: KRas is usually mutated in non-small cell lung cancer (NSCLC). The mutated KRas gene is a negative prognostic indicator that promotes tumor proliferation, metastasis, and drug resistance in NSCLC, and thus has become a target for cancer therapy. This study is focused on the effects of the microRNA (miR)-202/KRas axis in regulating chemosensitivity in NSCLC. Read More

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December 2018
1 Read

Cigarette Smoke Extract Promotes TIM4 Expression in Murine Dendritic Cells Leading to Th2 Polarization through ERK-Dependent Pathways.

Int Arch Allergy Immunol 2018 Dec 6:1-10. Epub 2018 Dec 6.

Laboratory of Clinical Immunology, Wuhan No. 1 Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan,

Smoking is considered to be the main source of indoor pollution, and it has been identified as an important environmental factor contributing to asthma onset. We know that T helper 2 (Th2) response plays a crucial role in the process of asthma disease. We have investigated the reaction of cigarette smoke extract (CSE) on Th polarization which is controlled by dendritic cells (DCs). Read More

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December 2018
3 Reads

In silico Leishmania proteome mining applied to identify drug target potential to be used to treat against visceral and tegumentary leishmaniasis.

J Mol Graph Model 2018 Nov 29;87:89-97. Epub 2018 Nov 29.

Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address:

New therapeutic strategies against leishmaniasis are desirable, since the treatment against disease presents problems, such as the toxicity, high cost and/or parasite resistance. As consequence, new antileishmanial compounds are necessary to be identified, as presenting high activity against Leishmania, but low toxicity in mammalian hosts. In the present study, a Leishmania proteome mining strategy was developed, in order to select new drug targets with low homology to human proteins, but that are considered relevant for the parasite' survival. Read More

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November 2018
1 Read

Hot spots for GPCR signaling: lessons from single-molecule microscopy.

Curr Opin Cell Biol 2018 Dec 3;57:57-63. Epub 2018 Dec 3.

Institute of Pharmacology and Bio-Imaging Center, University of Würzburg, Germany.

G protein-coupled receptors (GPCRs) are among the best-studied membrane receptors, mainly due to their central role in human physiology, involvement in disease and relevance as drug targets. Although biochemical and pharmacological studies have characterized the main steps in GPCR signaling, how GPCRs produce highly specific responses in our cells remains insufficiently understood. New developments in single-molecule microscopy have made it possible to study the protein-protein interactions at the basis of GPCR signaling in previously inconceivable detail. Read More

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December 2018
2 Reads

Simultaneous analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as potential plasma biomarkers to evaluate NSAIDs-induced gastric injury by liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2018 Nov 24;165:101-111. Epub 2018 Nov 24.

School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea. Electronic address:

Although major adverse effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) are gastric injury, assessment of NSAIDs-induced gastrointestinal adverse effects is mostly dependent on endoscopy due to the lack of plasma biomarkers. Several amino acids associated with collagenase activity and gastric mucosal mass have been suggested as plasma biomarker candidates for gastric injury. Therefore, this study aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the plasma biomarker candidates, i. Read More

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November 2018
2 Reads

Neuroprotective effects of a novel carnosine-hydrazide derivative on hippocampal CA1 damage after transient cerebral ischemia.

Eur J Med Chem 2018 Nov 28;163:207-214. Epub 2018 Nov 28.

Department of Neurosurgery, Kurume University, School of Medicine, Fukuoka, Japan. Electronic address:

Ischemia-reperfusion injuries produce reactive oxygen species that promote the peroxide lipid oxidation process resulting in the production of an endogenic lipid peroxide, 4-hydroxy-trans-2-nonenal (4-HNE), a highly cytotoxic aldehyde that induces cell death. We synthesized a novel 4-HNE scavenger - a carnosine-hydrazide derivative, l-carnosine hydrazide (CNN) - and examined its neuroprotective effect in a model of transient ischemia. PC-12 cells were pre-incubated with various doses (0-50 mmol/L) of CNN for 30 min, followed by incubation with 4-HNE (250 μM). Read More

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November 2018
1 Read

PD-1/ PD-L1 blockade as a novel treatment for colorectal cancer.

Biomed Pharmacother 2018 Dec 3;110:312-318. Epub 2018 Dec 3.

Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Colorectal cancer (CRC), as a prominent cause of cancer-related deaths, has historically been notable worldwide and many attempts have been made to raise the overall survival of CRC patients. Immune response has long been a question of great interest in a wide range of fields such as cancer therapies and anti-tumor immunity through checkpoint inhibitors, specifically anti PD-1/ PD-L1 interaction, is a new line of research for treatment of CRC patients. Following the successful development of anti-PD-1 for melanoma, renal cell carcinoma, and non-small cell lung cancer, several clinical trials have been conducted on monoclonal antibodies (MAbs) against PD-1 in CRC. Read More

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December 2018
9 Reads

Wuzhi capsule (Schisandra Sphenanthera extract) attenuates liver steatosis and inflammation during non-alcoholic fatty liver disease development.

Biomed Pharmacother 2018 Dec 3;110:285-293. Epub 2018 Dec 3.

Department of Organ Transplantation, ChangZheng Hospital, Second Military Medical University, Shanghai, 200003, China. Electronic address:

Objective: Wuzhi (WZ) capsule contains an ethanol extract of Schisandra sphenanthera. The efficacy of WZ in treating non-alcoholic fatty liver disease (NAFLD) has not yet been elucidated. The present study assessed the effects of WZ on NAFLD. Read More

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December 2018
2 Reads
2.023 Impact Factor

A PALB2 truncating mutation: Implication in cancer prevention and therapy of Hereditary Breast and Ovarian Cancer.

Breast 2018 Nov 29;43:91-96. Epub 2018 Nov 29.

Cancer Genetics Group, Institute of Genetics and Molecular Biology (UVa-CSIC), Sanz y Forés 3, 47003 Valladolid, Spain. Electronic address:

Explaining genetic predisposition in Hereditary Breast and Ovarian Cancer (HBOC) families without BRCA mutations is crucial. Germline PALB2 inactivating mutations were associated with an increased risk of HBOC due to its role in DNA repair through cooperation with BRCA proteins. The prevalence and penetrance of PALB2 mutations in Spanish HBOC patients remains unexplained. Read More

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November 2018
2 Reads

Physiological and biochemical effect of silver on the aquatic plant Lemna gibba L.: Evaluation of commercially available product containing colloidal silver.

Aquat Toxicol 2018 Nov 27;207:52-62. Epub 2018 Nov 27.

Josip Juraj Strossmayer University of Osijek, Department of Biology, Ulica cara Hadrijana 8/A, HR-31000, Osijek, Croatia. Electronic address:

This paper aims to evaluate the effects of a product containing colloidal silver in the aquatic environment, using duckweed Lemna gibba as a model plant. Therefore, growth parameters, photosynthetic pigments content and protein content as physiological indices were evaluated. Changes in the content of non-enzymatic antioxidants and activity of several antioxidant enzymes, alongside with the accumulation of hydrogen peroxide and lipid peroxidation end-products were assessed to explore the potential of colloidal silver to induce oxidative stress. Read More

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November 2018
1 Read

Interferons Transcriptionally Up-Regulate MLKL Expression in Cancer Cells.

Neoplasia 2018 Dec 3;21(1):74-81. Epub 2018 Dec 3.

Institute for Experimental Cancer Research in Pediatrics, Goethe-University, Frankfurt, Germany; German Cancer Consortium (DKTK), Partner Site, Frankfurt, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany. Electronic address:

Interferons (IFNs) are key players in the tumor immune response and act by inducing the expression of IFN-stimulated genes (ISGs). Here, we identify the mixed-lineage kinase domain-like pseudokinase (MLKL) as an ISG in various cancer cell lines. Both type I and type II IFNs increase the expression of MLKL indicating that MLKL up-regulation is a general feature of IFN signaling. Read More

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December 2018
1 Read

Proteomic analysis of olfactory bulb suggests CACNA1E as a promoter of CREB signaling in microbiota-induced depression.

J Proteomics 2018 Dec 3. Epub 2018 Dec 3.

Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing 400016, China; Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address:

Major depressive disorders impact approximately 17% of the population worldwide, whose high morbidity and considerable adversity have resulted in enormous social and economic burden. In addition, clinically depressed patients often show reduced volume of olfactory bulb (OB) and decreased olfactory sensitivity. Although mounting evidence conveyed that the gut microbiota may implicate the pathophysiology of major depressive disorder (MDD) via the microbe-gut-brain axis, knowledge about its distinctive molecular mechanism is rudimentary. Read More

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December 2018
2 Reads

Dirhodium (II) complex interferes with iron-transport system to exert antibacterial action against Streptococcus pneumoniae.

J Proteomics 2018 Dec 3. Epub 2018 Dec 3.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Drug resistance in bacteria is becoming a significant threat to global public health, and the development of novel and efficient antibacterial compounds is urgently needed. Recently, rhodium complexes have attracted attention as antimicrobial agents, yet their antibacterial mechanism remains unknown. In this study, we observed that the dirhodium (II) complex RhAc inhibited Streptococcus. Read More

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December 2018
1 Read

An integrated quantitative proteomic and metabolomics approach to reveal the negative regulation mechanism of LamB in antibiotics resistance.

J Proteomics 2018 Dec 3. Epub 2018 Dec 3.

Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, PR China. Electronic address:

Previously, a maltose-specific channel porin, LamB was found to be associate with multi-drug resistance in a lamB deleted strain, but the exact mechanisms require further elucidation. Herein, differential protein expression between the Escherichia coli mutant strain ΔlamB and the wild type strain BW25113, with and without ciprofloxacin (CFLX), was identified using iTRAQ based liquid chromatography-tandem mass spectrometry (LC-MS/MS); while differential metabolite expression was examined using gas chromatography-mass spectrometry (GC-MS). Further Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that lamB deletion leads to a decrease in several key metabolic pathways such as tricarboxylic acid (TCA) cycle, pentose phosphate pathway and glycolysis/gluconeogenesis. Read More

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December 2018

Enrichment of common carp (Cyprinus carpio) fingerlings diet with Psidium guajava: The effects on cutaneous mucosal and serum immune parameters and immune related genes expression.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address:

The present study aimed at evaluation of possible effects of Psidium guajava as immunostimulant on mucosal and serum immunity, as well as related genes expression in common carp (Cyprinus carpio) fingerlings. In an eight weeks feeding trial, fish were fed with experimental diets containing guava leaf powder [GLP] (0, 0.25, 0. Read More

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December 2018
5 Reads

TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, PR China.

CC chemokines are a large subfamily of chemokines that play an important role in the innate immune system. To date, several CC chemokines have been identified in fish species; however, the activities and functions of these putative chemokines remain ambiguous in teleosts, especially in the golden pompano, Trachinotus ovatus. Here, we characterized CC chemokine ligand 4 from T. Read More

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December 2018
2 Reads

An affinity peptide exerts antiviral activity by strongly binding nervous necrosis virus to block viral entry.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory of Improved Variety Reproduction in Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address:

Nervous necrosis virus (NNV) causes viral nervous necrosis (VNN), a disease that leads to almost 100% mortality among larvae and juvenile fish, severely affecting the aquaculture industry. VNN vaccines based on inactivated viruses or virus-like particles (VLPs) are unsuitable for fish fry with immature adaptive immune systems. Here, we applied an anti-NNV strategy based on affinity peptides (AFPs). Read More

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December 2018
1 Read

Long non-coding RNA THRIL promotes LPS-induced inflammatory injury by down-regulating microRNA-125b in ATDC5 cells.

Int Immunopharmacol 2018 Dec 3;66:354-361. Epub 2018 Dec 3.

Department of Orthopaedics, China-Japan Union Hospital of Jilin University, Changchun 130033, China. Electronic address:

Background: Osteoarthritis is an age-related disorder of bone-joint that causes pain and disability in middle and older people. This study aimed to investigate the potential effects of long non-coding RNA (lncRNA) THRIL on lipopolysaccharide (LPS)-induced osteoarthritis cell injury model (ATDC5 cell inflammatory injury), as well as the possible internal molecular mechanisms.

Methods: Cell viability and apoptosis were assessed using CCK-8 assay and Guava Nexin assay, respectively. Read More

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December 2018
2 Reads

Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix.

Int Immunopharmacol 2018 Dec 3;66:347-353. Epub 2018 Dec 3.

Department of orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. Electronic address:

Ivabradine is most commonly used for the treatment of worsening cardiac failure in patients who cannot tolerate the maximum dose of β-blockers or in whom treatment with β-blockers is contraindicated. While ivabradine is regarded as a highly selective "funny current" (I) inhibitor, the molecular mechanism behind the effect of this drug remains poorly understood. In the present study, we applied ivabradine in the context of osteoarthritis by treating primary human chondrocytes with tumor necrosis factor-α (TNF-α) and measuring degradation of the articular cartilage matrix as well as the expression of various enzymes and pro-inflammatory cytokines. Read More

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December 2018
4 Reads

Serum amyloid a as an indicator of impending xenograft failure: Experimental studies.

Int J Surg 2018 Dec 3. Epub 2018 Dec 3.

Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address:

Background: There is well-documented systemic inflammatory response in xenograft recipients to the presence of a pig graft. Serum amyloid A (SAA) is an inflammatory marker that is elevated in various pathological states. The assay used to measure it is (i) simple, (ii) relatively inexpensive, and (iii) provides an answer within minutes. Read More

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December 2018
3 Reads

Bipolar Disorder and 1513A>C P2RX7 polymorphism frequency.

Neurosci Lett 2018 Dec 3. Epub 2018 Dec 3.

Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil. Electronic address:

Although the etiology of Bipolar Disorder (BD) remains unknown, a strong genetic component to the pathogenesis and risk for this disorder has been widely hypothesized. Several risk genes for BD have been identified; of these, the purinergic P2 × 7 receptor (P2 × 7R) constitutes a pro-inflammatory receptor and a potential risk gene candidate. The purpose of the present study was to assess the frequency of the 1513 A > C P2RX7 polymorphism (rs3751143; Glu496Ala), which leads to receptor loss-of-function, in 154 BD patients versus 184 control subjects. Read More

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December 2018
1 Read

Identifying critical process steps to protein stability during spray drying using a vibrating mesh or a two-fluid nozzle.

Eur J Pharm Sci 2018 Dec 3. Epub 2018 Dec 3.

Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands. Electronic address:

The aim of this study was to identify critical steps to protein stability during spray drying using two different nozzle types: a vibrating mesh nozzle and a standard two-fluid nozzle in a Büchi B-90 spray dryer. l-Lactic dehydrogenase was used as a model protein as it is a heat and shear stress sensitive protein. Trehalose was used as excipient because of its excellent stabilizing capacities. Read More

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December 2018

Downregulation of RTN1-C attenuates MPP-induced neuronal injury through inhibition of mGluR5 pathway in SN4741 cells.

Brain Res Bull 2018 Dec 3. Epub 2018 Dec 3.

Department of Encephalopathy, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, Shaanxi, 710003, China. Electronic address:

Reticulons (RTNs) are a group of membrane-bound proteins that are dominantly localized to the endoplasmic reticulum (ER). RTN1-C, one isoform of RTNs highly expressed in the brain, has been shown to mediate neuronal injury in cerebral ischemia models. The aim of this study was to investigate the role of RTN1-C in an in vitro model of Parkinson's disease (PD) mimicked by 1-Methyl-4-phenylpyridinium (MPP) treatment in SN4741 cells. Read More

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December 2018
3 Reads

Extracellular FABP4 uptake by endothelial cells is dependent on cytokeratin 1 expression.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 3. Epub 2018 Dec 3.

Lipids and Atherosclerosis Research Unit, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili (IISPV), Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Universitat Rovira i Virgili, Reus, Spain. Electronic address:

Aims: The aim of this study is to determine the physical and functional interplay between fatty acid-binding protein 4 (FABP4) and its membrane receptor-like candidate protein, cytokeratin 1 (CK1), and to determine the effect of hindering CK1-mediated FABP4 cellular uptake on non-disturbed or metabolically stressed endothelial cells.

Methods: We monitored the direct interaction between FABP4 and CK1 using surface plasmon resonance, and the effects of blocking exogenous FABP4 (eFABP4) cellular uptake were determined by using specific siRNA to knock down the expression of CK1 in human umbilical vein endothelial cells (HUVECs). The expression and nuclear translocation of transcription factors involved in oxidative stress (NRF2) and inflammation (p65 subunit of NF-ĸB transcription factor) were determined by Western blotting analysis. Read More

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December 2018
1 Read

Patatin-like lipolytic acyl hydrolases and galactolipid metabolism in marine diatoms of the genus Pseudo-nitzschia.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 3. Epub 2018 Dec 3.

CNR-Istituto di Chimica Biomolecolare, Via Campi Flegrei 34, 80078, Pozzuoli, Naples, Italy. Electronic address:

Diatoms are eukaryotic microalgae that play a pivotal role in biological and geochemical marine cycles. These microorganisms are at the basis of the trophic chain and their lipids are essential components (e.g. Read More

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December 2018

Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X.

Behav Brain Res 2018 Dec 3. Epub 2018 Dec 3.

Program in Neuroscience, University of Arizona, Gould-Simpson Building, Tucson, AZ, 85721, United States; Department of Neuroscience, University of Arizona, Gould-Simpson Building, Tucson, AZ. 85721, United States; Department of Speech, Language, and Hearing Sciences, University of Arizona, Speech, Language, and Hearing Sciences Building, Tucson, AZ, 85721, United States. Electronic address:

Dopamine (DA) is an important neuromodulator of motor control across species. In zebra finches, DA levels vary in song nucleus Area X depending upon social context. DA levels are high and song output is less variable when a male finch sings to a female (female directed, FD) compared to when he is singing by himself (undirected, UD). Read More

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December 2018
1 Read

Exploring potential new galactomannan source of Retama reatam seeds for food, cosmetic and pharmaceuticals: Characterization and physical, emulsifying and antidiabetic properties.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Industrial Engineering, University of Salerno, Via Ponte Don Melillo, 84084 Fisciano, SA, Italy.

In this study, physicochemical, interfacial and emulsifying properties of Retama reatam and guar galactomannans were comparatively investigated. The results showed that Retama reatam galactomannan is mainly composed of total carbohydrates (95.52%) and lower protein contents (0. Read More

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December 2018

Characterization of polysaccharides isolated from Periploca angustifolia and its antioxidant activity and renoprotective potential against cadmium induced toxicity in HEK293 cells and rat kidney.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

University of Sfax Tunisia, Faculty of Sciences, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktonology, Unit UR 11 ES 72/Street of Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia.

Periploca angustifolia has numerous biological properties and also used against various diseases. In our study, this plant has been used for biotechnological production of polysaccharides. The prevention action of polysaccharides isolated from P. Read More

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December 2018
1 Read
2.858 Impact Factor

Mulberry leaf polysaccharides suppress renal fibrosis.

Authors:
Shengjun Wu

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, 59 Cangwu Road, Haizhou 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, 59 Cangwu Road, Haizhou 222005, China; Jiangsu Key Laboratory of Marine Biotechnology, 59 Cangwu Road, Haizhou 222005, China. Electronic address:

A mulberry leaf polysaccharide (MLP-1) was prepared and partially characterised, and its anti-renal-fibrosis effects were investigated in streptozotocin (STZ)-induced diabetic nephropathy (DN) rats. MLP-1, with a molecular weight of 2,792,624 Da, consisted of arabinose, glucose, xylose, rhamnose, and mannose in a molar ratio of 1:1.13:2. Read More

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December 2018
1 Read

Geodorum densiflorum rhizome lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of BAX, p53 and NF-κB genes expression.

Int J Biol Macromol 2018 Dec 4. Epub 2018 Dec 4.

Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh. Electronic address:

A lectin with a molecular mass of 12 ± 1 kDa was isolated for the first time from Geodorum densiflorum (Lam.) rhizome (GDL). The lectin exhibited hemagglutination activity both in mice and human erythrocytes which was inhibited by 4-nitrophenyl-β-D-glucopyranoside among the tested 26 sugars. Read More

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December 2018
1 Read
2.858 Impact Factor

Study of the selenizing Codonopsis pilosula polysaccharides protects RAW264.7 cells from hydrogen peroxide-induced injury.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address:

Codonopsis pilosula polysaccharides (CPPS) has been shown to possess a variety of biological activities. In previous study, CPPS was successfully modified to obtain its best selenizing Codonopsis pilosula polysaccharides (sCPPS). The purpose of this study was to investigate the protective effect of the selenizing derivative of CPPS (sCPPS) from HO-induced oxidative damage in RAW264. Read More

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December 2018
2 Reads

Chitosan-graft-poly(N-hydroxy ethyl acrylamide) copolymers: Synthesis, characterization and preliminary blood compatibility in vitro.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Biomedical Engineering, Near East University, Nicosia, P. O. Box: 99138, North Cyprus via Mersin 10, Turkey; Tissue Engineering and Biomaterials Research Centre, Near East University, P. O. Box: 99138, North Cyprus via Mersin 10, Turkey.

Poly(N-hydroxy ethyl acrylamide) was grafted onto chitosan in aqueous acidic medium using potassium per sulphate initiator to obtain polymer surfaces with blood compatibility for potential biomedical applications. Thermally cross-linked products were obtained upon drying at 60 °C overnight. Chemically cross-linked films were prepared using methylene bis acrylamide (MBA) cross-linker. Read More

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December 2018

Oxidation of cystatin imparted by riboflavin generated free radicals: Spectral analysis.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Biochemistry, Faculty of life sciences, Aligarh Muslim University, Aligarh, UP 202002, India. Electronic address:

Thiol Protease inhibitors (cystatins) are endogenous natural inhibitors of cysteine proteases. They are present in all mammalians cells and body fluids. Cystatin are allocated into three major families. Read More

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December 2018
3 Reads

Structural characterization, teratogenicity and in vitro avian antimicrobial activity of posterior salivary gland (PSG) toxin from cuttlefish, Sepia prashadi.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Native Medicine and Marine Pharmacology Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, Tamil Nadu, India. Electronic address:

A low molecular weight posterior salivary gland (PSG) toxin was isolated and purified from the cuttlefish Sepia prashadi by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). The protein and neutral sugar content of the PSG toxin was determined to be 1.033 mg/g and 282 μg/g. Read More

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December 2018

Pilot-scale isolation and characterization of extracellular polymeric substances (EPS) from cell-free medium of Spirulina sp. LEB-18 cultures under outdoor conditions.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This objective of this work was to monitor the EPS production during the growth of Spirulina sp. LEB-18, evaluate the productivity and to characterize the exopolymers obtained on pilot-scale under outdoor conditions. The production of crude EPS occurred in all phases of biomass growth and was approximately ten folds higher than that biomass concentration of Spirulina sp. Read More

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December 2018

Analysis of interaction between sulfated polysaccharides and HIV oligopeptides by surface plasmon resonance.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan. Electronic address:

This study aims to quantitatively investigate the interaction between sulfated polysaccharides with potent anti-HIV activity, dextran and curdlan sulfates with negatively charged sulfate groups, and poly-l-lysine as a model protein and oligopeptides from a HIV surface glycoprotein gp120 with positively charged amino acids using surface plasmon resonance (SPR) and dynamic light scattering (DLS) to elucidate the anti-HIV mechanism of sulfated polysaccharides. The apparent association- (k) and dissociation rate (k) constants of dextran and curdlan sulfates against poly-l-lysine were k = 6.92 × 10-2. Read More

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December 2018

Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388110, Gujarat, India.

Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest. Precise whole genome assembly and gene annotation are significant for gene functional analyses. Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir. Read More

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December 2018
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The novel cationic cell-penetrating peptide PEP-NJSM is highly active against Staphylococcus epidermidis biofilm.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour km 6, PO BOX 1177, 3018 Sfax, Tunisia.

A cationic cell-penetrating peptide PEP-NJSM was identified in human virus proteomes by a screening of charge clusters in protein sequences generating cell penetrating peptides (CPP). PEP-NJSM was selectively active against Gram positive Staphylococcus epidermidis as antibacterial agent with MIC value of 128 μM compared to the Gram-negative Pseudomonas aeruginosa strain with MIC value exceeded 512 μM. The selected peptide exhibited an important anti-biofilm activity even at sub-MIC levels. Read More

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December 2018
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Identification and molecular analysis of a lncRNA-HOTAIR transcript from secondary hair follicle of cashmere goat reveal integrated regulatory network with the expression regulated potentially by its promoter methylation.

Gene 2018 Dec 3. Epub 2018 Dec 3.

College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address:

The HOTAIR transcript is transcribed from the antisense strand within the HOXC gene cluster, and it is thought to play a role in regulating the inductive capacity of dermal papilla cells during the reconstruction of hair-follicle. In the current investigation, we firstly isolated and characterized a lncRNA-HOTAIR transcript from the secondary hair follicle of cashmere goat. Also, we analyzed its transcriptional pattern and methylation level of HOTAIR gene promoter in secondary hair follicle of cashmere goat during anagen and telogen stages. Read More

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December 2018
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RNA Therapy: Are we using the right Molecules?

Pharmacol Ther 2018 Dec 3. Epub 2018 Dec 3.

Department of Biochemistry & Molecular Medicine, UC Davis School of Medicine, Sacramento, CA 95817, USA.

Small-molecule and protein/antibody drugs mainly act on genome-derived proteins to exert pharmacological effects. RNA based therapies hold the promise to expand the range of druggable targets from proteins to RNAs and the genome, as evidenced by several RNA drugs approved for clinical practice and many others under active trials. While chemo-engineered RNA mimics have found their success in marketed drugs and continue dominating basic research and drug development, these molecules are usually conjugated with extensive and various modifications. Read More

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December 2018
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NEW INSIGHTS ON THE REGULATION OF CANCER CACHEXIA BY N-3 POLYUNSATURATED FATTY ACIDS.

Pharmacol Ther 2018 Dec 3. Epub 2018 Dec 3.

Institute of Physical Activity Sciences and Sports, Cruzeiro do Sul University, Sao Paulo, Brazil,; Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;. Electronic address:

Cancer cachexia is a multifactorial syndrome that develops during malignant tumor growth. Changes in plasma levels of several hormones and inflammatory factors result in an intense catabolic state, decreased activity of anabolic pathways, anorexia, and marked weight loss, leading to cachexia development and/or accentuation. Inflammatory mediators appear to be related to the control of a highly regulated process of muscle protein degradation that accelerates the process of cachexia. Read More

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December 2018
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