6,867,952 results match your criteria Protein science : a publication of the Protein Society[Journal]

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Dynll1 is essential for development and promotes endochondral bone formation by regulating intraflagellar Dynein function in primary cilia.

Hum Mol Genet 2019 Apr 22. Epub 2019 Apr 22.

St. Vincent's Institute of Medical Research.

Mutations in subunits of the cilia-specific cytoplasmic Dynein-2 (CD2) complex cause short-rib thoracic dystrophy syndromes (SRTDs), characterized by impaired bone growth and life-threatening perinatal respiratory complications. Different SRTD mutations result in varying disease severities. It remains unresolved whether this reflects the extent of retained hypomorphic protein functions or relative importance of the affected subunits for the activity of the CD2 holoenzyme. Read More

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http://dx.doi.org/10.1093/hmg/ddz083DOI Listing

Targeted Knock-In Mice with a Human Mutation in GRTH/DDX25 Reveals the Essential Role of Phosphorylated GRTH in Spermatid Development during Spermatogenesis.

Hum Mol Genet 2019 Apr 22. Epub 2019 Apr 22.

Section on Molecular Endocrinology, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) is a testis specific member of the DEAD-box family of RNA helicases expressed in meiotic and haploid germ cells which plays an essential role in spermatogenesis. There are two species of GRTH the 56 kDa non-phospho and 61 kDa phospho forms. Our early studies revealed a missense mutation (R242H) of GRTH in azoospermic men that when expressed in COS1-cells lack the phospho-form of GRTH. Read More

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https://academic.oup.com/hmg/advance-article/doi/10.1093/hmg
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http://dx.doi.org/10.1093/hmg/ddz079DOI Listing
April 2019
1 Read

Postnatal Development of Mice with Combined Genetic Depletions of Lamin A/C, Emerin and Lamina-associated Polypeptide 1.

Hum Mol Genet 2019 Apr 22. Epub 2019 Apr 22.

Department of Medicine.

Mutations in LMNA encoding lamin A/C and EMD encoding emerin cause cardiomyopathy and muscular dystrophy. Lmna null mice develop these disorders and have a lifespan of 7-8 weeks. Emd null mice show no overt pathology and have normal skeletal muscle but with regeneration defects. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/hmg/ddz082DOI Listing
April 2019
1 Read

Synthesis and immunological studies of β-1,2-mannan-peptide conjugates as antifungal vaccines.

Eur J Med Chem 2019 Apr 5;173:250-260. Epub 2019 Apr 5.

Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL, 32611, USA. Electronic address:

Fungal cell surface carbohydrates and proteins are useful antigens for the development of antifungal vaccines. In this study, glycopeptides consisting of the β-1,2-mannan and N-terminal peptide epitopes of Candida albicans (C. albicans) cell wall phosphomannan complex and Als1p (rAls1p-N) protein, respectively, were synthesized and covalently conjugated with keyhole limpet hemocyanin (KLH) and human serum albumin (HSA) through homobifunctional disuccinimidyl glutarate. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.04.001DOI Listing

Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy.

J Pharm Biomed Anal 2019 Apr 9;172:1-8. Epub 2019 Apr 9.

Jack Jumper Allergy Program, Royal Hobart Hospital, GPO Box 1061L, Hobart, Tasmania, 7001, Australia; School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, South Australia, 5001, Australia.

A major challenge in broader clinical application of Jack Jumper ant venom immunotherapy (JJA VIT) is the scarcity of ant venom which needs to be manually harvested from wild ants. Adjuvants are commonly used for antigen sparing in other vaccines, and thereby could potentially have major benefits to extend JJA supplies if they were to similarly enhance JJA VIT immunogenicity. The purpose of this study was to evaluate the physicochemical and microbiological stability and murine immunogenicity of low-dose JJA VIT formulated with a novel polysaccharide adjuvant referred to as delta inulin or Advax™. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.04.017DOI Listing

Role of nuclear RNA in regulating chromatin structure and transcription.

Curr Opin Cell Biol 2019 Apr 19;58:120-125. Epub 2019 Apr 19.

MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK. Electronic address:

The importance of three-dimensional chromatin organisation in genome regulation has never been clearer. But in spite of the enormous technological advances to probe chromatin organisation in vivo, there is still a lack of mechanistic understanding of how such an arrangement is achieved. Here we review emerging evidence pointing to an intriguing role of nuclear RNA in shaping large-scale chromatin structure and regulating genome function. Read More

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http://dx.doi.org/10.1016/j.ceb.2019.03.007DOI Listing

E3 ligase ASB8 promotes porcine reproductive and respiratory syndrome virus proliferation by stabilizing the viral Nsp1α protein and degrading host IKKβ kinase.

Virology 2019 Apr 15;532:55-68. Epub 2019 Apr 15.

School of Life Sciences, Tianjin University, Tianjin, 300072, China. Electronic address:

Porcine reproductive and respiratory syndrome virus (PRRSV) can potently suppress type I interferon production and escape from innate immune responses. PRRSV nonstructural protein 1α (Nsp1α) can inhibit IFN-β and NF-κB gene promoter activities, but the precise mechanisms are largely unclear. In this study, we demonstrated that PRRSV Nsp1α interacted with the host E3 ubiquitin ligase ankyrin repeat and SOCS box-containing 8 (ASB8). Read More

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http://dx.doi.org/10.1016/j.virol.2019.04.004DOI Listing

Identification of a human respiratory syncytial virus phosphoprotein domain required for virus-like-particle formation.

Virology 2019 Apr 9;532:48-54. Epub 2019 Apr 9.

Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK, 74078, USA. Electronic address:

Perceived inefficiency and inadequate knowledge of the human respiratory syncytial virus (hRSV) assembly process present a hurdle for large-scale production of authentic hRSV virus-like particles (VLPs) for vaccine purposes. We previously established that the matrix protein, phosphoprotein (P), and fusion protein carboxy-terminus were sufficient to generate VLPs that resemble filamentous wildtype hRSV. Here, the contribution of P was examined. Read More

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http://dx.doi.org/10.1016/j.virol.2019.04.001DOI Listing

Protein corona variation in nanoparticles revisited: A dynamic grouping strategy.

Colloids Surf B Biointerfaces 2019 Apr 3;179:505-516. Epub 2019 Apr 3.

Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Bio-nano interface investigation models are mainly based on the type of proteins present on corona, bio-nano interaction responses and the evaluation of final outcomes. Due to the extensive diversity in correlative models for investigation of nanoparticles biological responses, a comprehensive model considering different aspects of bio-nano interface from nanoparticles properties to protein corona fingerprints appeared to be essential and cannot be ignored. In order to minimize divergence in studies in the era of bio-nano interface and protein corona with following therapeutic implications, a useful investigation model on the basis of RADAR concept is suggested. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.003DOI Listing

Luteolin protects against diabetic cardiomyopathy by inhibiting NF-κB-mediated inflammation and activating the Nrf2-mediated antioxidant responses.

Phytomedicine 2018 Nov 28;59:152774. Epub 2018 Nov 28.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

Background: Diabetes mellitus is a well-known risk factor for the development of heart failure. Inflammation and oxidative stress play a key role in the development of diabetic cardiomyopathy (DCM), and this nexus represents an attractive target to combat this disease. Naturally occurring flavonoid luteolin exhibits both anti-inflammatory and antioxidant activities in various systems. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.11.034DOI Listing
November 2018

The enhancing immune response and anti-inflammatory effects of Anemarrhena asphodeloides extract in RAW 264.7 cells.

Phytomedicine 2018 Dec 13;59:152789. Epub 2018 Dec 13.

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea; Department of Korean Life Science and Technology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address:

Background: Anemarrhena asphodeloides has been widely used in traditional medicine for thousands of years; it has been reported to improve learning and memory, and to reduce inflammation. However, the role of A. asphodeloides in enhancing the immune response has remained unclear. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.12.012DOI Listing
December 2018

Vielanin P enhances the cytotoxicity of doxorubicin via the inhibition of PI3K/Nrf2-stimulated MRP1 expression in MCF-7 and K562 DOX-resistant cell lines.

Phytomedicine 2019 Mar 11;58:152885. Epub 2019 Mar 11.

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.

Background: Cancer cells that are resistant to structurally and mechanically unrelated anticancer drugs are said to have multidrug resistance (MDR). The overexpression of the ATP-binding cassette (ABC) transporter is one of the most important mechanisms of MDR. Vielanin P (VP), a dimeric guaiane from the leaves of Xylopia vielana, has the potential to reverse multidrug resistance. Read More

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http://dx.doi.org/10.1016/j.phymed.2019.152885DOI Listing

PML nuclear bodies are recruited to persistent DNA damage lesions in an RNF168-53BP1 dependent manner and contribute to DNA repair.

DNA Repair (Amst) 2019 Apr 6;78:114-127. Epub 2019 Apr 6.

Department of Genome Integrity, Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic; Laboratory of Genome Integrity, Institute of Molecular and Translational Medicine, Palacky University, Olomouc, Czech Republic; Genome Integrity Unit, Danish Cancer Society Research Center, Copenhagen, Denmark; Science for Life Laboratory, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 21, Stockholm, Sweden. Electronic address:

The bulk of DNA damage caused by ionizing radiation (IR) is generally repaired within hours, yet a subset of DNA lesions may persist even for long periods of time. Such persisting IR-induced foci (pIRIF) co-associate with PML nuclear bodies (PML-NBs) and are among the characteristics of cellular senescence. Here we addressed some fundamental questions concerning the nature and determinants of this co-association, the role of PML-NBs at such sites, and the reason for the persistence of DNA damage in human primary cells. Read More

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http://dx.doi.org/10.1016/j.dnarep.2019.04.001DOI Listing

Identification and bioactivity of a granulocyte colony-stimulating factor b homologue from large yellow croaker (Larimichthys crocea).

Fish Shellfish Immunol 2019 Apr 19. Epub 2019 Apr 19.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address:

Granulocyte colony-stimulating factor (GCSF) is a pleiotropic cytokine that plays a key role in regulation of hematopoiesis, innate and adaptive immune responses in mammals. However, bioactivity of GCSF in teleost fish remains largely unknown. In this study, a GCSFb homologue from large yellow croaker (Larimichthys crocea) (LcGCSFb) was cloned by RACE-PCR techniques. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193028
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http://dx.doi.org/10.1016/j.fsi.2019.04.048DOI Listing
April 2019
2 Reads

Metaproteomics of fecal samples of Crohn's disease and Ulcerative Colitis.

J Proteomics 2019 Apr 19. Epub 2019 Apr 19.

Bioprocess Engineering, Otto von Guericke University, Universitätsplatz 2, Magdeburg 39106, Germany. Electronic address:

Crohn's Disease (CD) and Ulcerative Colitis (UC) are chronic inflammatory bowel diseases (IBD) of the gastrointestinal tract. This study used non-invasive LC-MS/MS to find disease specific microbial and human proteins which might be used later for an easier diagnosis. Therefore, 17 healthy controls, 11 CD patients and 14 UC patients but also 13 Irritable Bowel Disease (IBS) patients, 8 Colon Adenoma (CA) patients, and 8 Gastric Carcinoma (GCA) patients were investigated. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.009DOI Listing

Stathmin regulates the proliferation and odontoblastic/ osteogenic differentiation of human dental pulp stem cells through Wnt/β-catenin signaling pathway.

J Proteomics 2019 Apr 19. Epub 2019 Apr 19.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, PR China; College of Stomatology, Southern Medical University, Guangzhou, PR China. Electronic address:

Osteogenic/ odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is a key factor in tooth and pulp regeneration, but its mechanism still remains unknown. The purpose of this research is to look into the mechanism by which Stathmin affects the proliferation and odontoblastic/ osteogenic differentiation of hDPSCs, and whether the Wnt/β- catenin is related to this regulation. First, the Stathmin expression was inhibited by lentiviral vector, after that the transcriptome sequencing technology was used to screen the differentially expressed genes, Wnt5a which related to the regulation of Wnt/β-catenin was regulated. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.014DOI Listing

Comparative proteomic analysis of two sesame genotypes with contrasting salinity tolerance in response to salt stress.

J Proteomics 2019 Apr 19. Epub 2019 Apr 19.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China. Electronic address:

Sesame is one of the most important oilseed crops and has high nutritional value. The yield and quality of sesame are severely affected by high salinity in coastal and semi-arid/arid regions. In this study, the phenotypic, physiological, and proteomic changes induced by salt treatment were analyzed in salt-tolerant (G441) and salt-sensitive (G358) seedlings. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.017DOI Listing

One-stage Surgical Treatment for Consecutive Multi-segment Thoracic Spinal Tuberculosis with Kyphosis by Posterior-only Debridement, Interbody Fusion and Instrumentation.

World Neurosurg 2019 Apr 19. Epub 2019 Apr 19.

Department of Spinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China. Electronic address:

Objective: To evaluate the clinical efficacy and feasibility of one-stage surgical treatment for consecutive multi-segment thoracic spinal tuberculosis with kyphosis by posterior-only debridement, interbody fusion and instrumentation.

Methods: Sixty-two patients who underwent posterior debridement, interbody fusion and instrumentation were reviewed for radiographic fusion, region kyphosis, neurological status and clinical outcomes. Thoracic Cobb's angle and Frankel grading system were employed to assess kyphosis and neurological improvements, respectively. Read More

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http://dx.doi.org/10.1016/j.wneu.2019.04.122DOI Listing

Nucleophosmin in leukemia: consequences of anchor loss.

Int J Biochem Cell Biol 2019 Apr 19. Epub 2019 Apr 19.

Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic. Electronic address:

Nucleophosmin (NPM), one of the most abundant nucleolar proteins, has crucial functions in ribosome biogenesis, cell cycle control, and DNA-damage repair. In human cells, NPM occurs mainly in oligomers. It functions as a chaperone, undergoes numerous interactions and forms part of many protein complexes. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.04.007DOI Listing

Revisiting SEPT7 and the slippage of β-strands in the septin family.

J Struct Biol 2019 Apr 19. Epub 2019 Apr 19.

São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, Brazil. Electronic address:

Septins are GTP-binding proteins that will often spontaneously assemble into filaments. In some species, particularly budding yeast, it is well known that these are capable of associating with membranes in order to fulfill their cellular role as a component of the cytoskeleton. Different from other human septins, SEPT7 appears to be unique in that it is an essential component of all hetero-oligomeric complexes described to date. Read More

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http://dx.doi.org/10.1016/j.jsb.2019.04.015DOI Listing

Origin and evolution of core components responsible for monitoring light environment changes during plant terrestrialization.

Mol Plant 2019 Apr 19. Epub 2019 Apr 19.

School of Advanced Agriculture Sciences and School of Life Sciences, State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing 100871, China. Electronic address:

Light serves as the source of energy as well as information signals for photosynthetic plants. During evolution, plants have acquired the ability to monitor environmental light radiation and adjust their developmental patterns to optimally utilize light energy for photosynthesis. The mechanisms of light perception and signal transduction have been comprehensively studied in the past decades, mostly in a few model plants, including Arabidopsis thaliana. Read More

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http://dx.doi.org/10.1016/j.molp.2019.04.006DOI Listing

Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation.

Food Chem Toxicol 2019 Apr 19. Epub 2019 Apr 19.

Yunnan Institute of Food Safety, Kunming University of Science and Technology, No. 727 South Jingming Road, Kunming, Yunnan, 650500, China. Electronic address:

Rambutan peel phenolics (RPP) have high antioxidant and anti-inflammatory activities. Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) possesses good radical scavenging activity and matrix metalloproteinase (MMPs) inhibitory ability. These underlying mechanisms indicated that RPP and LSGYGP may be used for antiphotoaging. Read More

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http://dx.doi.org/10.1016/j.fct.2019.04.020DOI Listing

The clinical impact of splanchnic ischemia in patients affected by thoracoabdominal aortic aneurysms treated with fenestrated and branched endograft.

Ann Vasc Surg 2019 Apr 19. Epub 2019 Apr 19.

Vascular Surgery, Department of Experimental, Diagnostic and Speciality Medicine, University of Bologna, Policlinico Sant'Orsola-Malpighi Bologna - Italy.

Introduction: Fenestrated/branched endografts (FB-EVAR) are valid options to treat thoraco-abdominal aortic aneurysms (TAAA). Successful repair requires target-visceral-vessels (TVV) manipulation with possible splanchnic ischemia. Aim of the study was to evaluate the clinical impact of splanchnic ischemia occurring in FB-EVAR for TAAA. Read More

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http://dx.doi.org/10.1016/j.avsg.2019.01.026DOI Listing

Interaction Between Microfilament and Microtubule- Dependent Tensions and Ischemia/Hypoxic-Induced Alteration of Structural Tension in Neuronal Cells.

Brain Res Bull 2019 Apr 19. Epub 2019 Apr 19.

Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing, PR China. Electronic address:

Intracellular mechanical tension plays a vital role in maintaining neuronal function and is generated steerablely by motor proteins along microfilaments (MFs) and microtubules (MTs). To explore the interaction between these subcellular tensions and elucidate their underlying mechanisms, we constructed MF- and MT-dependent tension probes using the Förster resonance energy transfer technique. Hypotonic stress activated MF and MT tensions in calcium-dependent manner, which antagonized outward expansion of cells synergistically; conversely, hypertonic stress attenuated MF and MT tensions in a calcium-independent manner and their interaction is antagonistical. Read More

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http://dx.doi.org/10.1016/j.brainresbull.2019.04.007DOI Listing

Significance of the red blood cell distribution width in resected pathological stage I non-small cell lung cancer.

Semin Thorac Cardiovasc Surg 2019 Apr 19. Epub 2019 Apr 19.

Department of Thoracic Surgery, Clinical Research Institute, National Hospital Organization, Kyushu Medical Center, 1-8-1 Jigyohama, Chuo-ku, Fukuoka 810-8563, Japan.

Background: Red blood cell distribution width (RDW) is a parameter measured in blood sample tests that reflects the variation in the volume of erythrocytes and used to differentiate an anemic state. The RDW has been used to predict a poor survival in various types of cancer; however, the prognostic impact of the RDW in resected pathological stage I non-small cell lung cancer (NSCLC) patients remains to be elucidated.

Methods: A total of 273 patients with resected pathological stage I NSCLC were included in this study. Read More

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http://dx.doi.org/10.1053/j.semtcvs.2019.04.011DOI Listing

The first complete mitochondrial genome of a cyclophorid land snail, with implications for architaenioglossan relationships (Mollusca, Caenogastropoda, Cyclophoroidea).

Int J Biol Macromol 2019 Apr 19. Epub 2019 Apr 19.

School of Life Sciences, Nanchang University, Nanchang 330031, China. Electronic address:

Herein, we described the complete mitochondrial genome ('mitogenome') of the Chinese land snail Cyclophorus martensianus, which is the first published mitogenome for the caenogastropod family Cyclophoridae. This mitogenome is 15,308 bp long consisting of 37 genes: 13 protein-coding genes (PCG's), 22 tRNA genes and two rRNA genes. The A + T content (69. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.138DOI Listing

Genome-wide annotation and comparative analysis of cuticular protein genes in the noctuid pest Spodoptera litura.

Insect Biochem Mol Biol 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing, 400716, China; Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400716, China. Electronic address:

Insect cuticle is considered an adaptable and versatile building material with roles in the construction and function of exoskeleton. Its physical properties are varied, as the biological requirements differ among diverse structures and change during the life cycle of the insect. Although the bulk of cuticle consists basically of cuticular proteins (CPs) associated with chitin, the degree of cuticular sclerotization is an important factor in determining its physical properties. Read More

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http://dx.doi.org/10.1016/j.ibmb.2019.04.012DOI Listing

Fibroblast growth factor 21 secretion enhances glucose uptake in mono(2-ethylhexyl)phthalate-treated adipocytes.

Toxicol In Vitro 2019 Apr 19. Epub 2019 Apr 19.

National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Miaoli 350, Taiwan. Electronic address:

Previous studies revealed that cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) disturbed energy metabolism in adipocytes, where glucose uptake was significantly increased. The present study aimed to determine the mechanisms underlying the increased glucose uptake. MEHP-treated 3T3-L1 adipocytes exhibited a significantly increased glucose uptake activity. Read More

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http://dx.doi.org/10.1016/j.tiv.2019.04.021DOI Listing

The blood proteomic signature of early-onset pediatric atopic dermatitis shows systemic inflammation and is distinct from adult, longstanding disease.

J Am Acad Dermatol 2019 Apr 19. Epub 2019 Apr 19.

Department of Dermatology, Northwestern University Feinberg School of Medicine, 676 North St. Clair Street, Chicago, IL 60611, USA. Electronic address:

Background: Despite increasing evidence that adults with longstanding atopic dermatitis (AD) have systemic inflammation, little is known about systemic inflammation in recent-onset early pediatric AD.

Objective: To analyze blood inflammatory proteins of early pediatric AD.

Methods: Using high-throughput proteomics (proximity extension assay), we assessed 257 inflammatory and cardiovascular risk proteins in blood of 30 children with moderate-to-severe AD <5 years of age (within 6 months of onset), compared to age-matched pediatric controls and adult AD. Read More

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http://dx.doi.org/10.1016/j.jaad.2019.04.036DOI Listing

Exercise preconditioning attenuates hind limb unloading-induced gastrocnemius muscle atrophy possibly via the HDAC4/Gadd45 axis in old rats.

Exp Gerontol 2019 Apr 19. Epub 2019 Apr 19.

Graduate School of Health and Sports Science, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba 270-1695, Japan. Electronic address:

The mechanisms involved in unloading-induced skeletal muscle loss may be age-specific, and the evidence for exercise preconditioning-induced protection against disuse muscle atrophy in aged rats is limited. Therefore, in this study, we investigated age-related differences in the activation of the HDAC4/Gadd45α pathway following hindlimb unloading (HU). We also assessed the protective effect of preconditioning exercise on this pathway in young and old rat gastrocnemius muscle. Read More

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http://dx.doi.org/10.1016/j.exger.2019.04.010DOI Listing

Streptozotocin causes acute responses on hippocampal S100B and BDNF proteins linked to glucose metabolism alterations.

Neurochem Int 2019 Apr 19. Epub 2019 Apr 19.

Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Streptozotocin (STZ) is a glucosamine-nitrosourea commonly used to induce long-lasting models of diabetes mellitus and Alzheimer's disease. Direct toxicity of STZ on the pancreas and kidneys has been well characterized, but the acute effect of this compound on brain tissue has received less attention. Herein, we investigated the acute and direct toxicity of STZ on fresh hippocampal slices, measuring changes in BDNF and S100B secretion (two widely-used peripheral markers of brain injury), as well as glucose metabolism. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.04.013DOI Listing

Protein nanotubes as state-of-the-art nanocarriers: Synthesis methods, simulation and applications.

J Control Release 2019 Apr 19. Epub 2019 Apr 19.

Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran. Electronic address:

Application of food proteins as a tool to form nanostructures (especially nanotubular shapes) has been an interesting topic for both the food and pharmaceutical sectors. Organic and protein nanostructures have better biocompatibility and biodegradability compared to inorganic counterparts like carbon nanotubes; in addition, they can undergo surface modifications. Several organic nanotubes have been developed, meanwhile, the engineered protein nanotubes in the food science have been prepared from α-lactalbumin, ovalbumin, cyclic peptide nanotubes, collagen, bovine serum albumin, lysozyme and hydrophobins which are of great interest to be applied in the food industry considering their outstanding properties. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.026DOI Listing

Plantamajoside attenuates isoproterenol-induced cardiac hypertrophy associated with the HDAC2 and AKT/ GSK-3β signaling pathway.

Chem Biol Interact 2019 Apr 19. Epub 2019 Apr 19.

Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, Liaoning, China; Emergency Medicine Department of General Hospital of Northern Theater Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, 83 Wenhua Road, Shenhe District, Shenyang, China. Electronic address:

As a compensatory response to cardiac overload, cardiac hypertrophy is closely associated with the occurrence and development of a variety of cardiovascular diseases, in which histone deacetylase 2 (HDAC2) has been reported to play an important role. Plantamajoside (PMS) is an active component extracted from Herba Plantaginis, which is a traditional Chinese medicine, and many biological activities of PMS have been reported. Here, we investigated the effects and mechanism of PMS on isoproterenol (ISO)-induced cardiac hypertrophy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00092797183173
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http://dx.doi.org/10.1016/j.cbi.2019.04.024DOI Listing

Ameliorative effect of zinc supplementation on compromised small intestinal health in streptozotocin-induced diabetic rats.

Chem Biol Interact 2019 Apr 19. Epub 2019 Apr 19.

Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysore, 570 020, India. Electronic address:

Zinc depletion during diabetes postulates a role for zinc nutrition in the management of associated complications. The present study evaluated zinc supplementation for countering the compromised intestinal integrity through moderation of oxidative stress and suppression of stress-stimulated inflammatory proliferation in streptozotocin-induced diabetic rats. Diabetic rats were provided with supplemental zinc for six weeks (5 and 10-times of normal level). Read More

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http://dx.doi.org/10.1016/j.cbi.2019.04.018DOI Listing

Inhibitory effect of mabuterol on proliferation of rat ASMCs induced by PDGF-BB via regulating [Ca]i and mitochondrial fission/fusion.

Chem Biol Interact 2019 Apr 19. Epub 2019 Apr 19.

School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China. Electronic address:

This study is aimed to investigate whether Mabuterol (Mab) inhibits proliferation of airway smooth muscle cells (ASMCs) induced by platelet-derived growth factor BB (PDGF-BB) and how far it is related to mitochondrial fission/fusion and intracellular calcium if it comes into play. To explore the mechanism of Mab's antagonizing the proliferation, Mdivi-1, DRP1 inhibitor, which has an inhibitory effect on mitochondrial fission, is used to compare with Mab. Cell viability was measured by either MTT or CCK-8. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.04.023DOI Listing

Dual actions of norathyriol as a new candidate hypouricaemic agent: uricosuric effects and xanthine oxidase inhibition.

Eur J Pharmacol 2019 Apr 19. Epub 2019 Apr 19.

Biomedical Engineering Research Center, Kunming Medical University, Kunming, 650500, China. Electronic address:

Hyperuricaemia, which results from the overproduction and underexcretion of uric acid, has been linked with chronic renal dysfunction, cardiovascular diseases, diabetes and metabolic syndrome. However, available clinical drugs cannot simultaneously target the production and excretion of uric acid. Norathyriol, a natural xanthone, was expected to have the dual actions. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.04.034DOI Listing

Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer.

Life Sci 2019 Apr 19. Epub 2019 Apr 19.

College of Pharmacy, Dalian Medical University, Dalian 116044, China. Electronic address:

Aims: To investigate the antitumor effect of 7-O-geranylquercetin (GQ) combining with survivin siRNA (siSuvi) or IL-10 siRNA (siIL-10) to breast cancer.

Main Methods: Xenograft tumor model was established by subcutaneously inoculating human breast cancer MCF-7 cells in BALB/c nude mice. Transfection efficiency of siRNA mediated by cationic liposome CDO14 in MCF-7 cells and tumor bearing mice was measured by flow cytometer and living imaging sysytem, respectively. Read More

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http://dx.doi.org/10.1016/j.lfs.2019.04.047DOI Listing

The sensilla trichodea-biased EoblPBP1 binds sex pheromones and green leaf volatiles in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones.

J Insect Physiol 2019 Apr 19. Epub 2019 Apr 19.

Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. Electronic address:

Pheromone-binding proteins (PBPs) are considered to play critical roles in sex pheromone detection. Lepidopteran moths can be divided into two taxa, those that use Type-I sex pheromones, such as C10-C18 unsaturated aldehydes, alcohols and acetates, and those that use Type-II pheromones, which are C17-C23 polyunsaturated hydrocarbons and their epoxide derivatives. To date, nearly all the characterized PBPs have been reported in moths with Type-I sex pheromones, and the physiological functions of PBPs in moths that use Type-II sex pheromones remains unclear. Read More

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http://dx.doi.org/10.1016/j.jinsphys.2019.04.005DOI Listing

Metabolic and Behavioral Responses of Predators to Prey Nutrient Content.

J Insect Physiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Integrative Biology, Oklahoma State University, 501 Life Science West, Stillwater, Oklahoma, 74075, USA.

Predators feed on a diversity of prey that can vary widely in nutrient content. While prey nutrient content is known to have important consequences for life history traits, less is known about how it affects physiology and behavior. The purpose of this study was to test how diet affected the physiology and behavior of the wolf spider Hogna carolinensis. Read More

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http://dx.doi.org/10.1016/j.jinsphys.2019.04.006DOI Listing

Relationship of α-MSH and AgRP axons to the perikarya of melanocortin-4 receptor neurons.

Brain Res 2019 Apr 19. Epub 2019 Apr 19.

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil. Electronic address:

The central melanocortin system is composed of neurons that express either the proopiomelanocortin (POMC) or the agouti-related protein (AgRP). POMC is cleaved in bioactive peptides, including the α-melanocyte-stimulating hormone (α-MSH). α-MSH activates the melanocortin-4 receptor (MC4R) inducing satiety, whereas AgRP acts as an inverse agonist of MC4R. Read More

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http://dx.doi.org/10.1016/j.brainres.2019.04.021DOI Listing

Knockout of adenylyl cyclase isoform 5 or 6 differentially modifies the β-adrenoceptor-mediated inotropic response.

J Mol Cell Cardiol 2019 Apr 19. Epub 2019 Apr 19.

Faculty of Medicine, Department of Pharmacology, Center for Heart Failure Research, Oslo University Hospital, University of Oslo, Oslo, Norway.

Although only β-adrenergic receptors (βAR) dually couple with stimulatory G protein (G) and inhibitory G protein (G), inactivation of G enhances both βAR and βAR responsiveness. We hypothesize that G restrains spontaneous adenylyl cyclase (AC) activity independent of receptor activation. Subcellular localization of the AC5/6 subtypes varies contributing to the compartmentation of βAR signaling. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2019.04.017DOI Listing

Expressional regulation of gonadotropin receptor genes and androgen receptor genes in the eel testis.

Gen Comp Endocrinol 2019 Apr 19. Epub 2019 Apr 19.

Department of Zoology, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand.

Receptors for follicle-stimulating hormone (Fshr), luteinizing hormone (Lhcgr1 and Lhcgr2) and androgens (Ara and Arb) transduce the hormonal signals that co-ordinate coordinate spermatogenesis, but the factors that regulate the abundance of these transducers in fish testes remain little-understood. To mend this paucity of information, we first determined changes in transcript abundance for these receptors (fshr, lhcgr1, ara and arb) during spermatogenesis induced by human chorionic gonadotropin (hCG) injection in the eel, Anguilla australis. We related our findings to testicular production of the fish androgen, 11-ketotestosterone (11-KT), and to the levels of the transcripts encoding steroidogenic acute regulatory protein (star) and 11β-hydroxylase (cyp11b), and subsequently evaluated the effects of hCG or 11-KT on mRNA levels of these target genes in vitro. Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.04.020DOI Listing

Serum soluble TWEAK levels in severe traumatic brain injury and its prognostic significance.

Clin Chim Acta 2019 Apr 19. Epub 2019 Apr 19.

Department of Critical Care Medicine, The First People's Hospital of Jiande City, 599 Yanzhou Main Road, Jiande 311600, China.

Background: Severe traumatic brain injury (sTBI) is characterized by a high mortality. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) participates in inflammation. We determined serum soluble TWEAK (sTWEAK) levels with respect to its prognostic ability. Read More

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http://dx.doi.org/10.1016/j.cca.2019.04.070DOI Listing

Functions of promyelocytic leukaemia zinc finger (Plzf) in male germline stem cell development and differentiation.

Reprod Fertil Dev 2019 Apr 23. Epub 2019 Apr 23.

Promyelocytic leukaemia zinc finger (Plzf), also known as zinc finger and BTB domain containing 16 (ZBTB16) or zinc-finger protein 145 (ZFP145), is a critical zinc finger protein of male germline stem cells (mGSCs). Multiple lines of evidence indicate that Plzf has a central role in the development, differentiation and maintenance of many stem cells, including mGSCs, and Plzf has been validated as an essential transcription factor for mammalian testis development and spermatogenesis. This review summarises current literature focusing on the significance of Plzf in maintaining and regulating self-renewal and differentiation of mGSCs, especially goat mGSCs. Read More

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http://dx.doi.org/10.1071/RD18252DOI Listing

The Role of Exopolysaccharides in Oral Biofilms.

J Dent Res 2019 Apr 22:22034519845001. Epub 2019 Apr 22.

1 Department of Oral Biology, Center for Oral Biology Research, Rutgers School of Dental Medicine, Newark, NJ, USA.

The oral cavity contains a rich consortium of exopolysaccharide-producing microbes. These extracellular polysaccharides comprise a major component of the oral biofilm. Together with extracellular proteins, DNA, and lipids, they form the biofilm matrix, which contributes to bacterial colonization, biofilm formation and maintenance, and pathogenesis. Read More

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http://dx.doi.org/10.1177/0022034519845001DOI Listing

Impact of Topology on the Characteristics of Water inside Cubic Lyotropic Liquid Crystalline Systems.

J Phys Chem B 2019 Apr 22. Epub 2019 Apr 22.

Water molecules present inside the lipid based cubic liquid crystalline phases are found to play a major role in wide range of applications, such as, protein crystallization, virus detection, delivery of drug and biomolecules etc. In this regard it is crucial to elucidate static and dynamic properties of the water molecules in the nanochannels and explore the effect of geometrical topology on the nature of the water inside the different cubic phases. In the present work, we have incorporated two probes, coumarin-343 (C-343) and coumarin-480 (C-480) in two cubic phases with different symmetries namely gyroid (Ia3d) and double diamond (Pn3m) with the same water content (22 %), to probe the micropolarity, microviscosity and the hydration dynamics at different hydrophobic depths in the mesophases. Read More

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http://dx.doi.org/10.1021/acs.jpcb.9b01559DOI Listing

High Homogenization Speeds for Preparing Unstable Myofibrillar Protein-Olive Oil Emulsions.

J Food Sci 2019 Apr 22. Epub 2019 Apr 22.

College of Food Science and Technology, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai Univ., Jinzhou, 121013, China.

Natural protein-based oil-in-water emulsions have recently attracted a lot of attention because of their potential as a synthetic emulsifier replacer. It is, however, unclear how the emulsification process and protein concentration may affect the stability of such emulsions. Therefore, this study investigated the effects of homogenization speeds (4,000, 8,000, 12,000, and 16,000 rpm/min) and myofibrillar protein (MP) concentrations (10, 20, 30, 40, and 50 mg/mL) on the stability of MP-olive oil emulsion. Read More

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http://dx.doi.org/10.1111/1750-3841.14502DOI Listing

Proteomics of intracellular Salmonella enterica reveals roles of Salmonella pathogenicity island 2 in metabolism and antioxidant defense.

PLoS Pathog 2019 Apr 22;15(4):e1007741. Epub 2019 Apr 22.

Abt. Mikrobiologie, Universität Osnabrück, Osnabrück, Germany.

Intracellular Salmonella enterica serovar Typhimurium (STM) deploy the Salmonella Pathogenicity Island 2-encoded type III secretion system (SPI2-T3SS) for the massive remodeling of the endosomal system for host cells. This activity results in formation of an extensive interconnected tubular network of Salmonella-induced filaments (SIFs) connected to the Salmonella-containing vacuole (SCV). Such network is absent in cells infected with SPI2-T3SS-deficient mutant strains such as ΔssaV. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007741DOI Listing

Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection.

PLoS Pathog 2019 Apr 22;15(4):e1007717. Epub 2019 Apr 22.

Department of Oral Health Sciences, University of Connecticut Health Center, Storrs, Connecticut, United States of America.

Infectious complications are a common cause of morbidity and mortality in cancer patients undergoing chemotherapy due to increased risk of oral and gastrointestinal candidiasis, candidemia and septicemia. Interactions between C. albicans and endogenous mucosal bacteria are important in understanding the mechanisms of invasive infection. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007717DOI Listing

Involvement of miR-770-5p in trastuzumab response in HER2 positive breast cancer cells.

PLoS One 2019 22;14(4):e0215894. Epub 2019 Apr 22.

Biotechnology Institute, Ankara University, Ankara, Turkey.

miRNAs may play effective roles in breast cancer so modulating their expression levels could have therapeutic benefits. Recent studies have found the combination of miRNA-based therapeutics with conventional drugs as promising. This study aimed to find drug-responsive miRNAs, and explore their anticancer activities in HER2+ breast cancer cells and regulatory role in the trastuzumab response. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215894PLOS
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