6,814,266 results match your criteria Protein science : a publication of the Protein Society[Journal]

  • Page 1 of 136286

Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation.

Redox Biol 2019 Feb 6;22:101137. Epub 2019 Feb 6.

Department of Molecular and Cellular Physiology, Albany Medical College, Albany, NY, United States. Electronic address:

Injury-induced stenosis is a serious vascular complication. We previously reported that p38α (MAPK14), a redox-regulated p38MAPK family member was a negative regulator of the VSMC contractile phenotype in vitro. Here we evaluated the function of VSMC-MAPK14 in vivo in injury-induced neointima hyperplasia and the underlying mechanism using an inducible SMC-MAPK14 knockout mouse line (iSMC-MAPK14). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.redox.2019.101137DOI Listing
February 2019

Composition of dissolved organic matter controls interactions with La and Al ions: Implications for phosphorus immobilization in eutrophic lakes.

Environ Pollut 2019 Feb 5;248:36-47. Epub 2019 Feb 5.

Nanjing Institute of Geography and Limnology, State Key Laboratory of Lake Science and Environment, Chinese Academy of Sciences, Nanjing, 210008, China.

Applications of aluminium (Al) salt or lanthanum (La) modified bentonite (LMB) have become popular methodologies for immobilizing phosphorus (P) in eutrophic lakes. The presence of humic substances, has been shown to inhibit this form of treatment due to the complexation with La/Al. However, the effects of other dissolved organic matter (DOM), especially that derived from phytoplankton (the dominant source in eutrophic lakes) are unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2019.02.002DOI Listing
February 2019

The influence of temperature on the effects induced by Triclosan and Diclofenac in mussels.

Sci Total Environ 2019 Jan 16;663:992-999. Epub 2019 Jan 16.

Instituto de Ciencias del Mar ICM-CSIC, E-08003 Barcelona, Spain.

Little is known about the consequences of exposure to pharmaceuticals and personal care products (PPCPs) in elevated temperatures associated with climate change. To increase the knowledge on this topic, Mytilus galloprovincialis mussels were exposed to 1.0μg/L of either the antimicrobial Triclosan (TCS) or the anti-inflammatory drug Diclofenac (DIC), at control (17°C) and 4°C raised (21°C) temperatures for 28days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.01.189DOI Listing
January 2019

Atherogenic cytokines and chemokines in chronic hepatitis C are not associated with the presence of cardiovascular diseases.

Cytokine 2019 Feb 13;115:24-31. Epub 2019 Feb 13.

Faculdade de Farmácia, Laboratório de Pesquisa em Imunologia, Universidade Federal da Bahia, Brazil. Electronic address:

There appears to be an associative link between chronic hepatitis C (CHC) and cardiovascular diseases (CVDs). However, the exact nature of the relationship between CHC and CVDs has not been elucidated. We investigated the presence of CVDs and the clinical and laboratory alterations associated with these diseases in CHC patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cyto.2018.12.005DOI Listing
February 2019

Pumping the brakes: suppression of synapse development by MDGA-neuroligin interactions.

Curr Opin Neurobiol 2019 Feb 13;57:71-80. Epub 2019 Feb 13.

Djavad Mowafaghian Centre for Brain Health and Department of Psychiatry, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC, V6T 2B5, Canada. Electronic address:

Synapse development depends on a dynamic balance between synapse promoters and suppressors. MDGAs, immunoglobulin superfamily proteins, negatively regulate synapse development through blocking neuroligin-neurexin interactions. Recent analyses of MDGA-neuroligin complexes revealed the structural basis of this activity and indicate that MDGAs interact with all neuroligins with differential affinities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.conb.2019.01.002DOI Listing
February 2019

Purification and biochemical characterization of a novel copper, zinc superoxide dismutase from liver of camel (Camelus dromedarius): An antioxidant enzyme with unique properties.

Bioorg Chem 2019 Feb 11;86:428-436. Epub 2019 Feb 11.

Chemistry Education, Kazim Karabekir Education Faculty, Atatürk University, 25240 Erzurum, Turkey.

A novel copper, zinc superoxide dismutase (CuZnSOD) was purified to homogeneity from the liver of an animal well adapted to the stressful living conditions of the desert, the camel (Camelus dromedarius). The biochemical properties of camel liver CuZnSOD were examined. The purified enzyme had a native molecular weight of 28 kDa, as judged by gel filtration chromatography, and showed a single band at 27 kDa on SDS-PAGE, indicating that it is a monomeric protein. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2019.02.024DOI Listing
February 2019

Unfractionated Heparin Alleviates Sepsis-Induced Acute Lung Injury by Protecting Tight Junctions.

J Surg Res 2019 Feb 13;238:175-185. Epub 2019 Feb 13.

Department of Critical Care Medicine, The First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China. Electronic address:

Background: Unfractionated heparin (UFH) has been shown to ameliorate lung edema and lung vascular leakage in lipopolysaccharide-induced lung injury. Impaired tight junction (TJ) function is a sign of sepsis-induced acute respiratory distress syndrome (ARDS) and acute lung injury (ALI), which is closely related to the downregulated expression of TJ-specific proteins or the upregulated expression of inflammatory cytokines. Because UFH has been intensively studied in modulating inflammation, we hypothesize that UFH may play a positive role in treating sepsis-induced ARDS/ALI by protecting TJs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2019.01.020DOI Listing
February 2019

Fish Oil Increases Specialized Pro-resolving Lipid Mediators in PAD (The OMEGA-PAD II Trial).

J Surg Res 2019 Feb 13;238:164-174. Epub 2019 Feb 13.

Division of Vascular and Endovascular Surgery, Department of Surgery, University of California, San Francisco, California. Electronic address:

Background: N-3 polyunsaturated fatty acid (PUFA) supplementation has been associated with reduced mortality and inflammation in patients with cardiovascular disease. There are limited data on the effects of n-3 PUFA supplementation in patients with peripheral artery disease (PAD).

Materials And Methods: The OMEGA-PAD II trial was a double-blinded, randomized, placebo-controlled trial to assess the effect of 3 mo of high-dose oral n-3 PUFA supplementation on inflammation, endothelial function, and walking ability in patients with PAD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2019.01.038DOI Listing
February 2019

SELDI-TOF MS Analysis of Hepatocellular Carcinoma in an Australian Cohort.

J Surg Res 2019 Feb 13;238:127-136. Epub 2019 Feb 13.

Cancer Surgery and Metabolism Research Group, University of Sydney, Kolling Institute of Medical Research, St Leonards, NSW, Australia; Upper GI Surgical Unit, Royal North Shore Hospital and North Shore Private Hospital, St Leonards, NSW, Australia; Discipline of Surgery, The University of Sydney, Camperdown, NSW, Australia.

Background: Hepatocellular carcinoma (HCC) is a common cause of cancer death worldwide. Resection offers the best chance of long-term survival, but a consistent adverse prognostic factor is the presence of microvascular invasion (MVI). In this study, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), a high throughput method of analyzing complex samples, was used to explore differentially expressed proteins between HCC and adjacent nontumour liver tissue (ANLT). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jss.2019.01.008DOI Listing
February 2019

Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway.

Ecotoxicol Environ Saf 2019 Feb 13;173:142-148. Epub 2019 Feb 13.

Department of Geriatric Endocrinology, Geriatric Hospital of Nanjing Medical University, Luojia Road 30, Nanjing 210024, China. Electronic address:

Microcystin-LR (MC-LR) is the most toxic and abundant microcystin that produced by cyanobacteria. Previous studies have shown MC-LR had acute toxic to thyroid, however, the mechanism is still unclear, and the effect of long-term, low-dose MC-LR on thyroid remains uncertain. In this study, we investigated the chronic, low-dose effect of MC-LR on mouse thyroid tissues and thyroid hormone metabolism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.02.024DOI Listing
February 2019

Melatonin alleviates aluminium chloride-induced immunotoxicity by inhibiting oxidative stress and apoptosis associated with the activation of Nrf2 signaling pathway.

Ecotoxicol Environ Saf 2019 Feb 13;173:131-141. Epub 2019 Feb 13.

Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The present study aimed to investigate whether melatonin (MT) treatment can attenuate immunotoxicity induced by aluminum chloride (AlCl) in rat spleen. Forty-eight healthy male Wistar rats were randomly allocated and treated with AlCl and/or MT. Rats were orally administered with AlCl for 90 days, from 61st days, rats were injected intraperitoneally with MT for 30 days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.01.095DOI Listing
February 2019

MHC I chaperone complexes shaping immunity.

Curr Opin Immunol 2019 Feb 13;58:9-15. Epub 2019 Feb 13.

Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt/Main, Germany. Electronic address:

Major histocompatibility complex class I (MHC I) molecules present peptides on the surface of most nucleated cells and allow the immune system to detect and eliminate infected or malignantly transformed cells. The peptides are derived from endogenous proteins by proteasomal degradation or aberrant translation, and are translocated from the cytosol into the endoplasmic reticulum (ER) by the transporter associated with antigen processing (TAP), a central component of the peptide-loading complex (PLC). The peptides are subsequently processed by ER-resident aminopeptidases (ERAP1/2) and loaded onto MHC I. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.coi.2019.01.001DOI Listing
February 2019

Clinical and genetic analysis of children with a dual diagnosis of Tourette syndrome and autism spectrum disorder.

J Psychiatr Res 2019 Feb 7;111:145-153. Epub 2019 Feb 7.

Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada. Electronic address:

Gilles de la Tourette Syndrome (TS) is a neurodevelopmental disorder that causes children to make repeated, brief involuntary movements or sounds. TS can be co-morbid with other neurodevelopmental disorders, including autism spectrum disorder (ASD). Clusters of biologically related genes have been associated with neurodevelopmental disorders, suggesting shared pathologies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpsychires.2019.01.023DOI Listing
February 2019

Parental exposure to tris(1,3-dichloro-2-propyl) phosphate results in thyroid endocrine disruption and inhibition of growth in zebrafish offspring.

Aquat Toxicol 2019 Feb 7;209:132-141. Epub 2019 Feb 7.

Key Laboratory of Preparation and Application of Environmental Friendly Materials, the Ministry of Education, Jilin Normal University, Siping, 136000, China.

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is a re-emerging environmental contaminant used as a suitable substitute for brominated flame retardants. The objective of this study was to evaluate the effects of TDCIPP on thyroid disruption and growth inhibition in zebrafish (Danio rerio) offspring after chronic parental exposure, and to examine the possible molecular mechanisms involved. When adult zebrafish (4 months old) were exposed to 5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aquatox.2019.02.004DOI Listing
February 2019

Dissymmetric pyridyl-substituted 3,5-bis(arylidene)-4-piperidones as anti-hepatoma agents by inhibiting NF-κB pathway activation.

Eur J Med Chem 2019 Feb 7;167:187-199. Epub 2019 Feb 7.

School of Pharmacy, The Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai, 264003, PR China. Electronic address:

To get new anti-hepatoma agents with anti-inflammatory activity and hypotoxicity, a series of dissymmetric pyridyl-substituted 3,5-bis(arylidene)-4-piperidones (BAPs, 25-82) were designed and synthesized. Many of them exhibited potential anti-hepatoma properties against human hepatocellular carcinoma cell lines (HepG2, QGY-7703, SMMC-7721) and hypotoxicity for human normal heptical cell line (HHL-5, LO2), and prominently inhibited lipopolysaccharides (LPS) induced IL-6, TNF-α secretion to exert its anti-inflammatory effect. Combining the data of cytotoxicity, cytocompatibility and anti-inflammatory activity, 3-pyridyl and -CF substituted 67 may be the potential anti-hepatoma agent. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2019.02.020DOI Listing
February 2019

Repurposing nitrocatechols: 5-Nitro-α-cyanocarboxamide derivatives of caffeic acid and caffeic acid phenethyl ester effectively inhibit aggregation of tau-derived hexapeptide AcPHF6.

Eur J Med Chem 2019 Feb 4;167:146-152. Epub 2019 Feb 4.

CIQUP/Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal. Electronic address:

Polyphenols like caffeic acid and its phenethyl ester have been associated with potent anti-aggregating activity. Accordingly, we screened a library of polyphenols and synthetic derivatives thereof for their capacity to inhibit tau-aggregation using a thioflavin T-based fluorescence method. Our results show that the nitrocatechol scaffold is required for a significant anti-aggregating activity, which is enhanced by introducing bulky substituents at the side chain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2019.02.006DOI Listing
February 2019

The effect of recycling culture medium after harvesting of Chlorella vulgaris biomass by flocculating bacteria on microalgal growth and the functionary mechanism.

Bioresour Technol 2019 Feb 8;280:188-198. Epub 2019 Feb 8.

College of Life Sciences, Henan Normal University, Xinxiang 453007, China; Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore 637141, Singapore. Electronic address:

Microalgal cultivation and harvesting are two main factors that restrict the development of microalgae. To help solve these two questions, flocculating bacteria and recycling culture medium were investigated in this study. Proteins in bacterial cells of Bacillus sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.01.149DOI Listing
February 2019

Identification and expression profiling of candidate chemosensory membrane proteins in the band-winged grasshopper, Oedaleus asiaticus.

Comp Biochem Physiol Part D Genomics Proteomics 2019 Feb 10;30:33-44. Epub 2019 Feb 10.

Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China. Electronic address:

The band-winged grasshopper Oedaleus asiaticus (Orthoptera: Acridoidea) is an important insect pest in north China. Chemosensory membrane proteins are believed to be crucial factors in direct interactions with odorants in the chemosensory process. However, there is much limited information on the chemosensory system in this pest. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbd.2019.02.002DOI Listing
February 2019

iTRAQ-based proteome profiling of hyposaline responses in zygotes of the Pacific oyster Crassostrea gigas.

Comp Biochem Physiol Part D Genomics Proteomics 2018 Dec 11;30:14-24. Epub 2018 Dec 11.

Fisheries College, Ocean University of China, Qingdao, Shandong, China.

Low salinity treatment is proven to be the practical polyploidy inducing method for shellfish with advantages of lower cost, higher operability and reliable food security. However, little is known about the possible molecular mechanism of hypotonic induction. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) based proteomic profiling was pursued to investigate the responses of zygotes of the Pacific oyster Crassostrea gigas to low salinity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbd.2018.12.003DOI Listing
December 2018

Different effects of single protein vs. protein mixtures on magnesium degradation under cell culture conditions.

Acta Biomater 2019 Feb 13. Epub 2019 Feb 13.

Helmholtz-Zentrum Geesthacht, Institute of Material Research, Max-Planck-Str. 1, 21502 Geesthacht, Germany. Electronic address:

Bovine serum albumin (BSA) or fetal bovine serum (FBS), as the protein component, is usually added into solution to study the influence of proteins on Mg degradation. However, the specific character of proteins used and the interaction between organic molecules in FBS do not draw enough attention. This study investigated the influence of BSA, fibrinogen (Fib) and FBS on Mg degradation in Hanks' balanced salt solution without (HBSS) or with calcium (HBSSCa) and Dulbecco's modified eagle medium Glutamax-I (DMEM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2019.02.013DOI Listing
February 2019

The Combination of MEK inhibitor with Immunomodulatory Antibodies Targeting PD-1 and PD-L1 Results in Prolonged Survival in Kras/p53-Driven Lung Cancer.

J Thorac Oncol 2019 Feb 13. Epub 2019 Feb 13.

Section of Medical Oncology, Department of Internal Medicine; Developmental Therapeutics Translational Research Program, Yale Comprehensive Cancer Center, New Haven, Connecticut , USA. Electronic address:

Purpose: To characterize the tumor-infiltrating immune cells population in Kras/p53-driven lung tumors and to evaluate the combinatorial anti-tumor effect with MEK inhibitor (MEKi), trametinib, and immunomodulatory monoclonal antibodies (mAbs) targeting either programmed cell death protein-1 (PD-1) or programmed cell death protein ligand 1 (PD-L1) in vivo.

Experimental Design: Trp53;Kras;Rosa26 (PKL) genetically engineered mice were utilized to develop autochthonous lung tumors with intratracheal delivery of adenoviral Cre recombinase. Using these tumor-bearing lungs, tumor-infiltrating immune cells were characterized by both mass cytometry and flow cytometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtho.2019.02.004DOI Listing
February 2019

Intravaginal treatment with Marantodes Pumilum (Kacip Fatimah) ameliorates vaginal atrophy in rats with post-menopausal condition.

J Ethnopharmacol 2019 Feb 13. Epub 2019 Feb 13.

Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address:

Ethnopharmacological Relevance: Marantodes pumilum (MP) (Kacip Fatimah) is used to maintain the well-being of post-menopausal women. However, its role in ameliorating post menopause-related vaginal atrophy (VA) is unknown.

Aims: To investigate the ability of intravaginal MP gel treatment to ameliorate VA in sex-steroid deficient condition, mimicking post-menopause. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2019.02.027DOI Listing
February 2019

Ascl1 Regulates Electric Field-Induced Neuronal Differentiation through PI3K/Akt Pathway.

Neuroscience 2019 Feb 13. Epub 2019 Feb 13.

Department of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun 130021, PR China. Electronic address:

Directing differentiation of neural stem/progenitor cells (NSCs/NPCs) to produce functional neurons is one of the greatest challenging in regenerative medicine. Our previous paper has confirmed that electrical stimulation has a high efficiency of triggering neuronal differentiation by using isolated filum terminale (FT) derived NPCs. To further clarify the intrinsic molecular mechanisms, protein-protein interaction (PPI) network analysis was applied to pinpoints novel hubs in electric field (EF)-induced neuronal differentiation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuroscience.2019.02.004DOI Listing
February 2019

Neurotoxic effects of MPTP on mouse cerebral cortex: Modulation of neuroinflammation as a neuroprotective strategy.

Mol Cell Neurosci 2019 Feb 13. Epub 2019 Feb 13.

Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal; UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal. Electronic address:

Parkinson's disease (PD) is a progressive neurological disorder, mainly characterized by the progressive loss of dopaminergic neurons in the Substantia nigra pars compacta (SNpc) and by the presence of intracellular inclusions, known as Lewy bodies. Despite SNpc being considered the primary affected region in PD, the neuropathological features are confined solely to the nigro-striatal axis. With disease progression other brain regions are also affected, namely the cerebral cortex, although the spreading of the neurologic damage to this region is still not completely unraveled. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mcn.2019.01.003DOI Listing
February 2019

BCG-induced formation of neutrophil extracellular traps play an important role in bladder cancer treatment.

Clin Immunol 2019 Feb 13. Epub 2019 Feb 13.

Tianjin Institute of Urology, Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, PR China.

Bacillus Calmette-Guerin (BCG) is one of the most effective treatments for bladder cancer. Little attention has been paid to the possible role of neutrophils in BCG immunotherapy. In this study, we examined neutrophil extracellular traps (NETs) formation induced by BCG stimulation, and found that BCG-induced NETs exerted cytotoxicity, induced apoptosis and cell-cycle arrest, and inhibited migration in bladder tumor cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clim.2019.02.005DOI Listing
February 2019

FMRpolyG alters mitochondrial transcripts level and respiratory chain complex assembly in Fragile X associated tremor/ataxia syndrome [FXTAS].

Biochim Biophys Acta Mol Basis Dis 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry, Faculty of Science, The M.S. University of Baroda, Vadodara 390002, Gujarat, India. Electronic address:

Fragile X-associated tremor/ataxia syndrome (FXTAS) is an inherited neurodegenerative disorder caused by an expansion of 55 to 200 CGG repeats (premutation) in FMR1. These CGG repeats are Repeat Associated non-ATG (RAN) translated into a small and pathogenic protein, FMRpolyG. The cellular and molecular mechanisms of FMRpolyG toxicity are unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbadis.2019.02.010DOI Listing
February 2019

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary.

Biochim Biophys Acta Mol Basis Dis 2019 Feb 13. Epub 2019 Feb 13.

Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy; Infertility Service, San Salvatore Hospital, L'Aquila 67100, L'Aquila, Italy. Electronic address:

Methylglyoxal (MG), a highly reactive dicarbonyl derived from metabolic processes, is the most powerful precursor of advanced glycation end products (AGEs). Glycative stress has been recently associated with ovarian dysfunctions in aging and PCOS syndrome. We have investigated the role of the NAD-dependent Class III deacetylase SIRT1 in the adaptive response to MG in mouse oocytes and ovary. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbadis.2019.02.011DOI Listing
February 2019

Prenatal exposure to pyrrolizidine alkaloids induced hepatotoxicity and pulmonary injury in fetal rats.

Reprod Toxicol 2019 Feb 13. Epub 2019 Feb 13.

Department of pharmacology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally Originated Diseases, Wuhan 430071, China. Electronic address:

Hepatic and pulmonary toxicity in fetal rats induced by pyrrolizidine alkaloids (PAs) was investigated. Retrorsine (RTS) or monocrotaline (MCT) was intragastrically administered during pregnancy. The reduction of body and tail lengths was consistent with body weight loss in PA-exposed fetuses, and pathological lesions in liver and lung were observed only in fetuses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.reprotox.2019.02.006DOI Listing
February 2019

In vitro toxicity assessment of zinc and nickel ferrite nanoparticles in human erythrocytes and peripheral blood mononuclear cell.

Toxicol In Vitro 2019 Feb 13. Epub 2019 Feb 13.

Nanobiology Laboratory, Department of Natural and Exact Sciences, Pontificia Universidad Católica Madre y Maestra, PUCMM, Autopista Duarte Km 1 ½, Santiago de los Caballeros, Dominican Republic. Electronic address:

Ferrite nanoparticles (NPs) have gained attention in biomedicine due to their many potential applications, such as targeted drug delivery, their use as contrast agents for magnetic resonance imaging and oncological treatments. The information about the risk effects of ferrite NPs in human blood cells is, however, scarce. To assess their potential toxicity, in vitro studies were carried out with magnetite and zinc, nickel and nickel‑zinc ferrites NPs at different concentrations (50, 100 and 200 μg·ml). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tiv.2019.02.011DOI Listing
February 2019

Engineered microbial host selection for value-added bioproducts from lignocellulose.

Biotechnol Adv 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address:

Lignocellulose is a rich and sustainable globally available carbon source and is considered a prominent alternative raw material for producing biofuels and valuable chemical compounds. Enzymatic hydrolysis is one of the crucial steps of lignocellulose degradation. Cellulolytic and hemicellulolytic enzyme mixes produced by different microorganisms including filamentous fungi, yeasts and bacteria, are used to degrade the biomass to liberate monosaccharides and other compounds for fermentation or conversion to value-added products. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biotechadv.2019.02.003DOI Listing
February 2019

Absolute configuration determination of asarinin by synchrotron radiation with crystalline sponge method.

Fitoterapia 2019 Feb 13. Epub 2019 Feb 13.

Key Laboratory on Protein Chemistry and Structural Biology, China Pharmaceutical University, Nanjing 210009, PR China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, PR China. Electronic address:

The determination of the absolute configuration of natural products still faces many challenges, especially the active pharmaceutical ingredient which is trace, oily and novel structures. Currently, NMR requires chiral reagents in determining the absolute configuration; ECD involves theoretical calculations and requires chromophores. In this study, the absolute configuration of asarinin had successfully identified by using synchrotron radiation with crystalline sponge method and combining MS with NMR. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fitote.2019.02.010DOI Listing
February 2019

Production of neutralizing antibodies against the secreted Clostridium chauvoei toxin A (CctA) upon blackleg vaccination.

Anaerobe 2019 Feb 13. Epub 2019 Feb 13.

Vetuisse Faculty, University of Bern, Bern, Switzerland. Electronic address:

Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the only efficient strategy in order to control the disease. The β-barrel pore forming leucocidin Clostridium chauvoei toxin A (CctA) is one of the best characterised toxins of C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.anaerobe.2019.02.011DOI Listing
February 2019

RLIP: An existential requirement for breast carcinogenesis.

Biochim Biophys Acta Rev Cancer 2019 Feb 13. Epub 2019 Feb 13.

Department of Internal Medicine, Division of Hematology & Oncology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.

Breast cancer (BC) is the most common cancer among women worldwide. Due to its complexity in nature, effective BC treatment can encounter many challenges. The human RALBP1 gene encodes a 76-kDa splice variant protein, RLIP (ral-binding protein1, RalBP1), a stress-protective mercapturic acid pathway (MAP) transporter protein, that also plays a key role in regulating clathrin-dependent endocytosis (CDE) as a Ral effector. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbcan.2019.02.001DOI Listing
February 2019

Fragile X mental retardation protein positively regulates PKA anchor Rugose and PKA activity to control actin assembly in learning/memory circuitry.

Neurobiol Dis 2019 Feb 13. Epub 2019 Feb 13.

Vanderbilt Brain Institute, Departments of Biological Sciences, Cell and Developmental Biology, and Pharmacology, Vanderbilt University and Medical Center, Nashville, TN 37235, USA. Electronic address:

Recent work shows Fragile X Mental Retardation Protein (FMRP) drives the translation of very large proteins (>2000 aa) mediating neurodevelopment. Loss of function results in Fragile X syndrome (FXS), the leading heritable cause of intellectual disability (ID) and autism spectrum disorder (ASD). Using the Drosophila FXS disease model, we discover FMRP positively regulates the translation of the very large A-Kinase Anchor Protein (AKAP) Rugose (>3000 aa), homolog of ASD-associated human Neurobeachin (NBEA). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nbd.2019.02.004DOI Listing
February 2019

Comparative transcriptomics reveals mechanisms underlying cln3-deficiency phenotypes in Dictyostelium.

Cell Signal 2019 Feb 13. Epub 2019 Feb 13.

Department of Biology, Trent University, Peterborough, Ontario, Canada.

Mutations in CLN3 cause a juvenile form of neuronal ceroid lipofuscinosis (NCL). This devastating neurological disorder, commonly known as Batten disease, is currently untreatable due to a lack of understanding of the physiological role of the protein. Recently, work in the social amoeba Dictyostelium discoideum has provided valuable new insight into the function of CLN3 in the cell. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2019.02.004DOI Listing
February 2019

Expanding the promoter toolbox of Bacillus megaterium.

J Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry, Campus B2.2, 66123, Saarland University, Saarbrücken, Germany. Electronic address:

Over the past decades, Bacillus megaterium has gained significant interest in the biotechnological industry due to its high capacity for protein production. Although many proteins have been expressed efficiently using the optimized xylose inducible system so far, there is a considerable demand for novel promoters with varying activities, particularly for the adjustment of protein levels in multi-enzyme cascades. Genome-wide microarray analyses of the industrially important B. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2019.01.018DOI Listing
February 2019

A Two-Amino Acid Mutation in Murine IgA Enables Downstream Processing and Purification on Staphylococcal Superantigen-Like Protein 7.

J Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

Ghent University, Department of Plant Biotechnology and Bioinformatics, VIB Center for Plant Systems Biology, 9052 Ghent, Belgium; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC), Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain. Electronic address:

With few exceptions, all currently marketed antibody therapeutics are IgG molecules. One of the reasons that other antibody isotypes are less developed are the difficulties associated with their purification. While commercial chromatography affinity resins like staphylococcal superantigen-like 7 (SSL7) protein containing resin, allow purification of IgAs from many animal species, these are not useful for murine IgAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2019.01.020DOI Listing
February 2019

Characterization of a novel antibacterial N-acyl amino acid synthase from soil metagenome.

J Biotechnol 2019 Feb 13. Epub 2019 Feb 13.

Department of Bio-industry and Bio-resource Engineering, Sejong University, Seoul, 05006, Republic of Korea. Electronic address:

In an effort to isolate novel natural antibiotics, we searched for antibacterial long-chain N-acyl amino acid synthase (NAS) genes from 70,000 soil metagenome clones by Bacillus subtilis-overlaying screening. In an antibacterial cosmid clone, YS92B, a single gene nasYPL was responsible for the production of the Nas. nasYPL was 903 bp long, and the deduced amino acid sequence showed the highest 71% identity with a hypothetical protein from Massilia niastensis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2019.01.017DOI Listing
February 2019

Aloe emodin induces hepatotoxicity by activating NF-κB inflammatory pathway and P53 apoptosis pathway in zebrafish.

Toxicol Lett 2019 Feb 13. Epub 2019 Feb 13.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, National Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, 611137, China. Electronic address:

The aim of this study was to investigate the hepatotoxic effect and its underlying mechanism of aloe emodin (AE). AE was docked with the targets of NF-κB inflammatory pathway and P53 apoptosis pathway respectively by using molecular docking technique. To verify the results of molecular docking and further investigate the hepatotoxicity mechanism of AE, the zebrafish Tg (fabp10: EGFP) was used as an animal model in vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxlet.2019.02.007DOI Listing
February 2019

Targeted demethylation of the SARI promotor impairs colon tumour growth.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan, 610041, PR China. Electronic address:

SARI (suppressor of activator protein 1, regulated by IFN) functions as a tumour suppressor and is inactivated in various cancers. However, the mechanism underlying SARI inactivation in cancer remains elusive. In this study, we detected a high level of DNA methylation of the SARI promoter and an inverse correlation between SARI promoter methylation and expression in malignant tissues from patients with colon cancer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2019.01.040DOI Listing
February 2019

Metformin Induces Human Esophageal Carcinoma Cell Pyroptosis by Targeting the miR-497/PELP1 Axis.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Institute of Precision Cancer Medicine and Pathology, And Department of Pathology, Jinan University Medical College, Guangzhou, China; Department of Immunotherapy and Tumor Tissue Bank, Affiliated Cancer Hospital of Shantou University Medical College, Shantou, Guangdong, China. Electronic address:

Evasion of apoptosis is a major contributing factor to the development of chemo- and radiotherapy resistance. Therefore, activation of non-apoptotic programmed cell death (PCD) could be an effective alternative against apoptosis-resistant cancers. In this study, we demonstrated in vitro and in vivo that metformin can induce pyroptosis, a non-apoptotic PCD, in esophageal squamous cell carcinoma (ESCC), a commonly known chemo-refractory cancer, especially at its advanced stages. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2019.02.014DOI Listing
February 2019

ProNGF increases breast tumor aggressiveness through functional association of TrkA with EphA2.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Inserm, U908, F-59000, Lille, France; Univ. Lille, U908 - CPAC, Cell Plasticity and Cancer, F-59000, Lille, France. Electronic address:

ProNGF expression has been linked to several types of cancers including breast cancer, and we have previously shown that proNGF stimulates breast cancer invasion in an autocrine manner through membrane receptors sortilin and TrkA. However, little is known regarding TrkA-associated protein partners upon proNGF stimulation. By proteomic analysis and proximity ligation assays, we found that proNGF binding to sortilin induced sequential formation of the functional sortilin/TrkA/EphA2 complex, leading to TrkA-phosphorylation dependent Akt activation and EphA2-dependent Src activation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2019.02.019DOI Listing
February 2019

Identification and characterization of small molecule inhibitors of the ubiquitin ligases Siah1/2 in melanoma and prostate cancer cells.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA. Electronic address:

Inhibition of ubiquitin ligases with small molecule remains a very challenging task, given the lack of catalytic activity of the target and the requirement of disruption of its interactions with other proteins. Siah1/2, which are E3 ubiquitin ligases, are implicated in melanoma and prostate cancer and represent high-value drug targets. We utilized three independent screening approaches in our efforts to identify small-molecule Siah1/2 inhibitors: Affinity Selection-Mass Spectrometry, a protein thermal shift-based assay and an in silico based screen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2019.02.012DOI Listing
February 2019

Long non-coding RNA FGF13-AS1 inhibits glycolysis and stemness properties of breast cancer cells through FGF13-AS1/IGF2BPs/Myc feedback loop.

Cancer Lett 2019 Feb 13. Epub 2019 Feb 13.

Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, China; Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Harbin, China. Electronic address:

LncRNAs have been proven to play crucial roles in various processes of breast cancer. LncRNA FGF13-AS1 has been identified as one of the 25 downregulated lncRNAs in breast cancer through analyzing data from two cohorts and TCGA by another group of our lab. In this study, we report that FGF13-AS1 expression is decreased in breast cancer tissue compared with corresponding normal tissue, and the downregulation of FGF13-AS1 is associated with poor prognosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.canlet.2019.02.008DOI Listing
February 2019

Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9.

J Control Release 2019 Feb 13. Epub 2019 Feb 13.

Cancer Biology Division, Department of Radiation Oncology, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8224, St. Louis, MO 63110, USA; Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8224, St. Louis, MO 63110, USA. Electronic address:

Hemophilia B (HB) is a life-threatening inherited disease caused by mutations in the FIX gene, leading to reduced protein function and abnormal blood clotting. Due to its monogenic nature, HB is one of the primary targets for gene therapy. Indeed, successful correction of HB has been shown in clinical trials using gene therapy approaches. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2019.02.009DOI Listing
February 2019

A combined immunodeficiency with severe infections, inflammation and allergy caused by ARPC1B deficiency.

J Allergy Clin Immunol 2019 Feb 13. Epub 2019 Feb 13.

Emma Children's Hospital, Amsterdam UMC, University of Amsterdam, Department of Pediatric Immunology, Rheumatology and Infectious diseases, Meibergdreef 9, Amsterdam, The Netherlands; Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory AMC, University of Amsterdam, The Netherlands. Electronic address:

We report the natural history, clinical manifestations, genetics, and immunohematological findings in 14 patients from 11 families with ARPC1B deficiency, delineating the spectrum of the disease that appears progressive and challenging to manage clinically. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaci.2019.02.003DOI Listing
February 2019

Identification of small molecule inhibitors targeting the Zika virus envelope protein.

Antiviral Res 2019 Feb 13. Epub 2019 Feb 13.

Department of Microbiology and Blavatnik Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. Electronic address:

The recent emergence of Zika virus, a mosquito-borne flavivirus, in the Americas has shed light on the severe neurological diseases associated with infection, notably congenital microcephaly in newborns and Guillain-Barré syndrome in adults. Despite the recent focus on Zika virus, there are currently no approved vaccines or antiviral therapies available to treat or prevent infection. In this study we established a competitive amplified luminescent proximity homogeneous assay (ALPHAscreen) to identify small molecule inhibitors targeting the envelope protein of Zika virus (Zika E). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.antiviral.2019.02.008DOI Listing
February 2019

Baloxavir marboxil susceptibility of influenza viruses from the Asia-Pacific, 2012-2018.

Antiviral Res 2019 Feb 13. Epub 2019 Feb 13.

WHO Collaborating Centre for Reference and Research on Influenza, VIDRL, Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia; University of Melbourne, Department of Microbiology and Immunology, Parkville, VIC 3010, Australia. Electronic address:

Baloxavir Marboxil (BXM) is an influenza polymerase inhibitor antiviral that binds to the endonuclease region in the PA subunit of influenza A and B viruses. To establish the baseline susceptibility of viruses circulating prior to licensure of BXM and to monitor for susceptibility post-BXM use, a cell culture-based focus reduction assay was developed to determine the susceptibility of 286 circulating seasonal influenza viruses, A(H1N1)pdm09, A(H3N2), B (Yamagata/Victoria) lineage viruses, including neuraminidase inhibitor (NAI) resistant viruses, to Baloxavir Acid (BXA), the active metabolic form of BXM. BXA was effective against all influenza subtypes tested with mean EC values (minimum-maximum) of 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.antiviral.2019.02.007DOI Listing
February 2019

Inhibition of HBsAg secretion by nucleic acid polymers in HepG2.2.15 cells.

Antiviral Res 2019 Feb 13. Epub 2019 Feb 13.

INRS-Institut Armand-Frappier, Institut National de la Recherche Scientifique, Laval, Canada. Electronic address:

More than 290 million people have chronic HBV infection and are at risk of developing cirrhosis and hepatocellular carcinoma. HBV subviral particles are produced in large excess over virions in infected patients and are the primary source of HBsAg, which is postulated to be important in allowing HBV to chronically persist by interfering with immune function. Nucleic acid polymers (NAPs) have been shown to result in clearance of HBsAg from the blood in pre-clinical and clinical studies. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.antiviral.2019.02.009DOI Listing
February 2019

Evaluation of structural, functional, and anti-oxidant potential of differentially extracted polysaccharides from potatoes peels.

Int J Biol Macromol 2019 Feb 13. Epub 2019 Feb 13.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang seasoning Food Co., Ltd., Jieshou 236500, People's Republic of China. Electronic address:

Potato peel was used for the extraction of three types of polysaccharides (PW, PAL, and PAC) using water, alkaline, and acid treatments, respectively. The structure of the PP polysaccharides was examined by means of Fourier transform infrared spectroscopy (FT-IR) analysis and Gas chromatography-mass spectrometry (GC-MS). The results suggest that the extracted polysaccharides constituted essentially three functional groups; CO, CH, and OH. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.02.074DOI Listing
February 2019
  • Page 1 of 136286