Potential of EPR spin-trapping to investigate in situ free radicals generation from skin allergens in reconstructed human epidermis: cumene hydroperoxide as proof of concept.
- Salen Kuresepi,
- Bertrand Vileno,
- Philippe Turek,
- Jean-Pierre Lepoittevin,
- Elena Giménez-Arnau
Free Radic Res 2018 Jan 15:1-9. Epub 2018 Jan 15.
a Dermatochemistry Laboratory , University of Strasbourg, CNRS UMR 7177, Institut le Bel , Strasbourg , France.
The first step in the development of skin sensitisation to a chemical, and in the elicitation of further allergic contact dermatitis (ACD), is the binding of the allergen to skin proteins after penetrating into the epidermis. The so-formed antigenic adduct is then recognised by the immune system as foreign to the body. Sensitising organic hydroperoxides derived from autoxidation of natural terpenes are believed to form antigens through radical-mediated mechanisms, although this has not yet been established. Read More