6,763,824 results match your criteria Protein Contact Dermatitis
Sportverletz Sportschaden 2018 Dec 11;32(4):243-250. Epub 2018 Dec 11.
Muscle Research Center Erlangen, Interdisciplinary Center for Muscle Research, Friedrich-Alexander-University Erlangen-Nuremberg, Germany.
Delayed-onset muscle soreness (DOMS) is a type of ultrastructural muscle injury. The manifestation of DOMS is caused by eccentric or unfamiliar forms of exercise. Clinical signs include reduced force capacities, increased painful restriction of movement, stiffness, swelling, and dysfunction of adjacent joints. Read More
PLoS One 2018 11;13(12):e0205052. Epub 2018 Dec 11.
School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuda-cho, Midori-ku, Yokohama, Kanagawa, Japan.
The principle of three-dimensional protein structure formation is a long-standing conundrum in structural biology. A globular domain of a soluble protein is formed by a network of atomic contacts among amino acid residues, but regions without intramolecular non-local contacts are often observed in the protein structure, especially in loop, linker, and peripheral segments with secondary structures. Although these regions can play key roles for protein function as interfaces for intermolecular interactions, their nature remains unclear. Read More
Kidney Blood Press Res 2018 Dec 7;43(6):1822-1831. Epub 2018 Dec 7.
Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung,
Background/aims: Hyperglycemia and hyperuricemia are two major disorders of Metabolic syndrome. Kidney plays a crucial role in maintaining the homeostasis of uric acid and glucose. The aim of the study was to examine the changes of renal glucose and uric acid transporters in animals with metabolic syndrome. Read More
Cell Physiol Biochem 2018 11;51(5):2472-2483. Epub 2018 Dec 11.
Spine Tumor Center, Department of Orthopedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Background/aims: Giant cell tumor of bone (GCTB), one of the most common primary bone tumors, leads to extensive bone destruction. However, the mechanisms underlying GCTB progression remain elusive and prognostic factors and treatment targets are required. In the current study, we explored the function of the chemokine family member CCL20 in GCTB progression. Read More
Cell Physiol Biochem 2018 11;51(5):2456-2471. Epub 2018 Dec 11.
Background/aims: Skin photoaging is primarily caused by the functional attrition of skin stem cells. The skin stem cell niche plays an important role in maintaining stem cell survival and behaviour. In our study, we hypothesized that UVB irradiation induces skin photoaging by changing skin stem cell niches and that transferred adipose-derived stem cells (ADSCs) can remodel the niches by affecting the BMP signalling pathway and transdifferentiating into skin stem cells. Read More
Cell Physiol Biochem 2018 11;51(5):2421-2433. Epub 2018 Dec 11.
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
Background/aims: Excessive fluoride intake can induce cytotoxicity, DNA damage and cell-cycle changes in many tissues and organs, including the kidney. However, the underlying molecular mechanisms of fluoride-induced renal cell-cycle changes are not well understood at present. In this study, we used a mouse model to investigate how sodium fluoride (NaF) induces cell-cycle changes in renal cells. Read More
Cell Physiol Biochem 2018 7;51(5):2341-2358. Epub 2018 Dec 7.
Key Laboratory of the Model Animal Research, Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing,
Background/aims: This study investigated the effect of consecutive superovulation on the ovaries and established a premature ovarian failure (POF) model in mice.
Methods: The mouse POF model was induced by 5-15 consecutive superovulation treatments with pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) and prostaglandin F2α (PGF2α). Normal adult mice were compared with mice displaying natural ovarian aging. Read More
Cell Physiol Biochem 2018 7;51(5):2324-2340. Epub 2018 Dec 7.
Department of Prevention Medicine, School of Public Health, Mudanjiang Medical College, Mudanjiang City,
Background/aims: Circular RNAs (circRNAs) are key regulators in the development and progression of human cancers, however its role in non-small cell lung cancer (NSCLC) tumorigenesis is not well understood. The aim of this study is to identify the expression level of circPVT1 in NSCLC and further investigated its functional relevance with NSCLC progression both in vitro and in vivo.
Methods: Quantative real-time PCR was used for the measurement of circPVT1 in NSCLC specimens and cell lines. Read More
Cell Physiol Biochem 2018 7;51(5):2309-2323. Epub 2018 Dec 7.
Department of Orthopedic Surgery, ShengJing Hospital, China Medical University, ShenYang,
Background/aims: Chondrocyte apoptosis is a central pathological feature of cartilage in osteoarthritis (OA). Accumulating evidence suggests that calcium ions (Ca2+) are an important regulator of apoptosis. Previously, we reported that the transient receptor potential channel vanilloid (TRPV5) is upregulated in monoiodoacetic acid (MIA)-induced OA articular cartilage. Read More
Cell Physiol Biochem 2018 7;51(5):2290-2308. Epub 2018 Dec 7.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou,
Background/aims: Increasing evidence has demonstrated the novel roles of mesenchymal stem cells (MSCs) in immunotherapy. However, difficulty in acquiring these cells and possible ethical issues limited their application. Recently, we have isolated a unique MSC population from dental follicles with potent stem cell-like properties. Read More
Cell Physiol Biochem 2018 7;51(5):2275-2289. Epub 2018 Dec 7.
Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming,
Background/aims: Parkinson's disease (PD) is a frequently occurring condition that resulted from the loss of midbrain neurons, which synthesize the neurotransmitter dopamine. In this study, we established mouse models of PD to investigate the expression of microRNA-128 (miR-128) and mechanism through which it affects apoptosis of dopamine (DA) neurons and the expression of excitatory amino acid transporter 4 (EAAT4) via binding to axis inhibition protein 1 (AXIN1).
Methods: Gene expression microarray analysis was performed to screen differentially expressed miRNAs that are associated with PD. Read More
Cell Physiol Biochem 2018 7;51(5):2224-2236. Epub 2018 Dec 7.
Departments of Urology, the Third Affiliated Hospital of SooChow University, Changzhou,
Background/aims: We aimed to study the involvement of circZMYM2 (hsa_circ_0099999) in pancreatic cancer (PC) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circZMYM2 regulating miR-335-5p and JMJD2C.
Methods: CircRNA differential expressions in twenty PC samples and paired normal tissue samples were analyzed using Arraystar Human CircRNA microarray V1. CircZMYM2 expression level was determined via qRT-PCR. Read More
Cell Physiol Biochem 2018 7;51(5):2185-2197. Epub 2018 Dec 7.
Department of Medicine, The University of Chicago, Chicago, Illinois,
Background/aims: VCP-interacting membrane selenoprotein (VIMP), an ER resident selenoprotein, is highly expressed in β-cells, however, the role of VIMP in β-cells has not been characterized. In this study, we studied the relationship between VIMP deficiency and β-cell survival in MIN6 insulinoma cells.
Methods: To determine the role of VIMP in β-cells, lentiviral VIMP shRNAs were used to knock down (KD) expression of VIMP in MIN6 cells. Read More
Cell Physiol Biochem 2018 7;51(5):2172-2184. Epub 2018 Dec 7.
Department of Immunology, Binzhou Medical University, Yantai,
Background/aims: Although red blood cells (RBCs) transfusions can be lifesaving, they are not without risk. RBCs storage is associated with the abnormal metabolism of glutathione (GSH), which may increase the risk of the oxidative damage of RBCs after transfusion. The responsible mechanisms remain unknown. Read More
Oncology 2018 Dec 11:1-7. Epub 2018 Dec 11.
Department of Pathology, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland.
Objectives: Protein levels of the transcription factor nuclear factor erythroid-derived 2-like 2 (Nrf2) and its inhibitor Kelch-like ECH-associated protein 1 (Keap1) have been proposed as prognostic factors in pancreatic ductal adenocarcinomas (PDACs). These cellular redox-state-regulating enzymes are targeted by several microRNAs, including miR-93 and miR-200a.
Methods: We assessed mRNA levels of Nrf2 and Keap1 and tissue expression of miR-93 and miR-200a in 51 patients with surgically treated PDAC. Read More
Cardiorenal Med 2018 Dec 11;9(2):69-82. Epub 2018 Dec 11.
Harold Simmons Center of Kidney Disease Research and Epidemiology, Division of Nephrology and Hypertension, University of California Irvine School of Medicine, Orange, California,
Background: Residual kidney function (RKF) is often expected to inevitably and rapidly decline among hemodialysis patients and, hence, has been inadvertently ignored in clinical practice. The importance of RKF has been revisited in some recent studies. Given that patients with end-stage renal disease now tend to initiate maintenance hemodialysis therapy with higher RKF levels, there seem to be important opportunities for incremental hemo-dialysis by individualizing the dose and frequency according to their RKF levels. Read More
Obes Facts 2018 Dec 11;11(6):440-453. Epub 2018 Dec 11.
Nutrition Postgraduate Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal,
Objective: This study evaluated the effect of a protein, the isolated Trypsin Inhibitor (TTI) from Tamarindus indica L. seed, as a CCK secretagogue and its action upon food intake and leptin in obese Wistar rats.
Methods: Three groups of obese rats were fed 10 days one of the following diets: Standard diet (Labina®) + water; High Glycemic Index and Load (HGLI) diet + water or HGLI diet + TTI. Read More
Kidney Blood Press Res 2018 Dec 7;43(6):1842-1851. Epub 2018 Dec 7.
Background/aims: We aimed to explore the associations between clinical parameters and long-term allograft outcomes in transplant glomerulopathy (TG) in a large retrospective cohort with long follow-up.
Methods: Clinical and laboratory data at biopsy from 180 cases of TG with an estimated glomerular filtration rate (eGFR)> 15ml/min/1.73m2 from January 2004 to December 2016 at our center were retrospectively analyzed. Read More
Biomed Pharmacother 2018 Dec 8;110:602-608. Epub 2018 Dec 8.
Department of Orthopedics, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, Hubei, 430060, China. Electronic address:
Background: Osteoblasts play important roles in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone (Dex), a type of glucocorticoids (GCs), induces apoptosis of osteoblasts and leads to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of phosphatidylinositol 3-kinase/Protein kinase 3 (PI3K/AKT) and glycogen synthase kinase 3β (GSK3β) on Dex-induced osteoblasts apoptosis. Read More
Biomed Pharmacother 2018 Dec 8;110:594-601. Epub 2018 Dec 8.
Institute of Immunopathology and Preventive Medicine, Povsetova ulica 29, 1000 Lublana, Slovenia. Electronic address:
Pharmacological interventions which could be hepatoprotective, depending on bioavailability, anti-inflammatory and macrophage-targeting potential of drugs, are still at early preclinical stages. Existing evidence from many animal models of liver injury, as well as from human data, indicate that pharmacological and/or phytochemical interventions have limited impact on liver recovery. Recent studies on stem cell therapies focused on different cell subsets involved in tissue repair, including monocytes/macrophages and bone marrow cells migrating to the injured liver. Read More
Biomed Pharmacother 2018 Dec 7;110:571-581. Epub 2018 Dec 7.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China. Electronic address:
Background And Aims: Non-alcoholic steatohepatitis (NASH) is the hepatic manifestation of metabolic syndrome and is characterized by steatosis, inflammation, and fibrosis. We aim to characterize the hepatoprotective effects of Leonurine hydrochloride (LH) and the possible pathway in a cell and rodent model of diet-induced steatohepatitis (NASH).
Methods: For in vitro studies, Palmitic acid (PA) and free fatty acid (FFA) induced HepG2 and HL7702 steatosis cell models were used. Read More
Biomed Pharmacother 2018 Dec 7;110:561-570. Epub 2018 Dec 7.
Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan, 750004, China. Electronic address:
Spermatogenic dysfunction is a common complication in men with diabetes and is the most important manifestation of diabetes-related male reproduction damage. Astragalin (AG) is one of the main flavonoids from Cuscuta chinensis, which has rich pharmacological activities. This study aimed to establish whether AG may contribute to the recovery from spermatogenic dysfunction. Read More
J Colloid Interface Sci 2018 Dec 3;538:489-498. Epub 2018 Dec 3.
Department of Chemistry, Arba Minch University, Ethiopia.
Herein, we have explored the conformational alterations of hemoglobin (Hb) in presence of a cleavable gemini surfactant (C16-C4O2-C16). The concerned surfactant was found to induce significant structural perturbations in Hb. UV-vis spectroscopy, steady-state/time-resolved fluorescence, and other utilized techniques have authenticated the complexation of Hb with the gemini surfactant. Read More
Theriogenology 2018 Nov 26;126:81-87. Epub 2018 Nov 26.
Laboratory of Molecular Biology, Faculty of Animal Science, University of Sao Paulo, São Paulo, Brazil.
This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage. Read More
Int Immunopharmacol 2018 Dec 8;67:98-105. Epub 2018 Dec 8.
Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea. Electronic address:
The anti-inflammatory effects of 3‑bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol (BEMB) from Polysiphonia morrowii were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells and zebrafish embryo. BEMB showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS), and the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in the LPS-activated RAW264. Read More
Int Immunopharmacol 2018 Dec 8;67:78-86. Epub 2018 Dec 8.
Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, PR China. Electronic address:
Oxidative stress and neuroinflammation are thought to be the two key early events during the process of mild cognitive impairment (MCI). Therefore, effective regulation of oxidative stress and neuroinflammation is an important aspect of preventing and improving MCI. We previously found that pseudoginsenoside-F11 (PF11), an ocotillol-type saponin, markedly reduced cognitive impairment in APP/PS1 mice and oAβ-injected mice. Read More
Colloids Surf B Biointerfaces 2018 Dec 4;175:221-230. Epub 2018 Dec 4.
Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran. Electronic address:
C-Phycocyanin pigment was purified from a native cyanobacterial strain using a novel consecutive multi-step procedure and utilized for the first time for the green synthesis of phycocyanin-zinc oxide nanorods (PHY-ZnO NRs) by a simple, low-cost and eco-friendly approach. The PHY-ZnO NRs were characterized using UV-vis spectroscopy, X-ray diffraction (XRD), zeta potential measurement, FTIR, SEM, TEM, differential scanning calorimetry (DSC), thermogravimetric (TGA), and EDX spectroscopy analysis. The UV-vis spectra showed an absorption band at 364 nm which is characteristic of ZnO nanoparticles (ZnONPs). Read More
J Plant Physiol 2018 Nov 20;232:94-99. Epub 2018 Nov 20.
Institute of Basic Biological Problems of Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
The carrier of carbonic anhydrase (CA) activity was detected in gel among low molecular mass proteins from pea, spinach and Arabidopsis, after nondenaturing electrophoresis in PAAG of the dodecyl-β-d-maltoside treated PSII membranes (the fragments of thylakoid membrane containing PSII complexes). The elimination of Mn-stabilizing protein PsbO by treatment of PSII membranes with salts, did not lead to a decrease in CA activity observed in the gel although it reduced the amount of this protein down to 25% compared to the original sample. The isolated protein PsbO did not demonstrated CA activity. Read More
J Plant Physiol 2018 Nov 20;232:74-81. Epub 2018 Nov 20.
Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland.
The expression of SlNP24 encoding osmotin was studied in germinating tomato seeds Solanum lycopersicum L. cv. Moneymaker. Read More
J Plant Physiol 2018 Nov 19;232:61-64. Epub 2018 Nov 19.
Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Krakow, Poland.
The aim of present study was to evaluate the proteolytic response of metallicolous (M) and nonmetallicolous (NM) ecotypes of Alyssum montanum under heavy metals (HMs) stress. Therefore, shoot cultures of tested species grown on medium enriched simultaneously with 0.7 mM ZnSO, 3. Read More
J Plant Physiol 2018 Nov 22;232:216-225. Epub 2018 Nov 22.
MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Shandong Agricultural University, Tai-An, Shandong, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong, China. Electronic address:
In plants, SIZ1 regulates abiotic and biotic stress responses by promoting the SUMOylation of proteins. The apple MdSIZ1 protein has conserved domains similar to those of Arabidopsis AtSIZ1. Real-time fluorescent quantitative analysis showed that MdSIZ1 gene expression was induced by phosphate-deficient conditions. Read More
J Plant Physiol 2018 Nov 10;232:200-208. Epub 2018 Nov 10.
Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 31008, China. Electronic address:
Tea flowering in late autumn competes for a large amount of nitrogen and carbohydrates, potentially undermines the storage of these resources in vegetative organs, and negatively influences the subsequent spring tea yield and quality. The mechanism underlying the re-allocation N and carbohydrate from source leaf to flower in tea plant has not been clearly understood. In this study, N allocation, changes in metabolomics, and gene expression in flower buds, flowers, and adjacent leaves were characterized. Read More
J Plant Physiol 2018 Nov 13;232:180-187. Epub 2018 Nov 13.
Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan. Electronic address:
The plant hormone jasmonic acid (JA) plays an important role in defense response and plant development. Jasmonate ZIM-domain (JAZ) proteins act as transcriptional repressors of plant responses to JA. In this study, we found that OsNINJA1, which is a JAZ-interacting adaptor protein, plays an important role in JA signaling that is positively regulated by the transcription factor OsMYC2 in rice. Read More
J Plant Physiol 2018 Nov 22;232:151-159. Epub 2018 Nov 22.
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address:
UV radiation is a serious threat to life, and algae have developed highly efficient adaptations to UV radiation through the course of evolution. To date, studies investigating the mechanisms of UV adaptation in algae have focused on physiological regulation and associated protein coding genes, with only a few reports on associated protein non-coding genes. In a previous study, we found that Cre-miR914 was significantly down-regulated in Chlamydomonas reinhardtii in response to heat shock. Read More
J Plant Physiol 2018 Nov 16;232:141-150. Epub 2018 Nov 16.
Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou Hainan 571101, China; College of Life Sciences, Key Laboratory for Ecology of Tropical Islands, Ministry of Education, Hainan Normal University, Haikou, Hainan 571158, China. Electronic address:
Sesuvium portulacastrum, an important mangrove-associated true halophyte belongs to the family Aizoaceae, has excellent salt tolerance. Chloroplasts are the most sensitive organelles involved in the response to salinity. However, the regulation mechanism of chloroplasts of S. Read More
J Plant Physiol 2018 Nov 3;232:127-129. Epub 2018 Nov 3.
N.I. Vavilov Institute of General Genetics Russian Academy of Sciences, Gubkina str. 3, Moscow, 119333, Russian Federation.
The scope of RNA-aptamers application is becoming wider and has expanded beyond solely medical use. We propose the use of RNA-aptamers in plants to suppress the functions of individual proteins, thereby achieving resistance to various biotic and abiotic stresses. In current work we experimentally demonstrate the possibility of inhibiting protein activity in N. Read More
J Plant Physiol 2018 Nov 10;232:115-126. Epub 2018 Nov 10.
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Lignans are diphenolic compounds produced in plants via coupling of two coniferyl alcohol molecules with the aid of a dirigent protein to form pinoresinol (PINO). The latter is reduced via lariciresinol (LARI) to secoisolariciresinol by the bifunctional pinoresinol-lariciresinol reductase (PLR). In this study, we clarified the consequences of altered lignan biosynthesis on amino acids, phenolics compounds and lignin in the hairy roots of Linum album with an ihpRNAi construct to silence PLR gene expression. Read More
Chemosphere 2018 Dec 5;219:155-164. Epub 2018 Dec 5.
State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China. Electronic address:
Deltamethrin is widely used because of its low toxicity and high efficiency. Although its potential toxicity has been reported, its effects on cardiovascular system and motor behavior and its underlying mechanisms have remained unclear. In this study, the effects of deltamethrin on the development, cardiovascular system and motor behavior of zebrafish larvae and their possible mechanisms were evaluated using the transgenic zebrafish Tg (kdrl:mCherry) and Tg(myl7:GFP). Read More
Environ Toxicol Pharmacol 2018 Dec 3;65:53-59. Epub 2018 Dec 3.
Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand; Translational Medicine Graduate Program, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. Electronic address:
Cadmium is a highly neurotoxic heavy metal impairing neurogenesis and induces neurodegenerative disorders. Toxic concentrations of cadmium induce astrocytic apoptosis by depleting intracellular glutathione levels, elevating intracellular calcium levels, altering mitochondria membrane potentials, and activating JNK and PI3K/Akt signaling pathways. Cadmium suppresses cell proliferation in kidney epithelial cells, lung fibroblasts, and primary myelocytes; however, cadmium's effects on proteins regulating oxidative stress, apoptosis, and cell proliferation in astrocytes are less known. Read More
Biol Blood Marrow Transplant 2018 Dec 8. Epub 2018 Dec 8.
Diagnostic Laboratories and Blood Research Institute, BloodCenter of Wisconsin, part of Versiti, Milwaukee, WI. Electronic address:
HLA matching by allele-level genotyping is largely based on genetic similarity between a few exons which encode the antigen recognition domain (ARD) of the HLA protein. Next-generation sequencing (NGS) can identify HLA genetic polymorphisms in non-ARD encoding exons, introns, and untranslated regions, but the impact of these polymorphisms on hematopoietic cell transplant (HCT) outcomes is unclear. We performed NGS-based sequencing of eleven HLA loci on a well-characterized retrospective cohort of 166 unrelated donor/recipient HCT pairs. Read More
J Steroid Biochem Mol Biol 2018 Dec 8. Epub 2018 Dec 8.
School of Medicine, Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. Electronic address:
In the vitamin D intervention study VitDbol (NCT02063334) blood samples were drawn directly before an oral bolus (2000 µg vitamin D) and 24 h later. The focus of phase II of VitDbol was the transcriptome-wide analysis of the effects of vitamin D gene expression in human peripheral blood mononuclear cells (PBMCs). All five participants responded in an individual fashion to the bolus by increases in serum levels of the vitamin D metabolites 25-hydroxyvitamin D (25(OH)D) and 1α,25-dihydroxyvitamin D (1,25(OH)D). Read More
Fish Shellfish Immunol 2018 Dec 8. Epub 2018 Dec 8.
Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China. Electronic address:
Glutaredoxin (Grx) is a class molecule oxidoreductase, which can regulate the redox state of proteins and plays a key role in antioxidant defense. However, the informations of Grx cDNA sequences and their functions are lack in decapod crustacea. In the present study, the cDNA of LvGrx 2 was cloned from the Pacific white shrimp, Litopenaeus vannamei. Read More
Neuroscience 2018 Dec 8. Epub 2018 Dec 8.
Department of Biology & Center for Tissue Regeneration and Engineering at Dayton (TREND), University of Dayton, Dayton, Ohio, USA. Electronic address:
Preclinical evidence suggests that ketamine's rapid and sustained antidepressant actions are due to the induction of synaptogenesis in the medial prefrontal cortex (mPFC) and the hippocampus (HIPP), two brain regions implicated in the pathophysiology of major depression. However, research on the neurobiological effects of ketamine has focused almost exclusively on males. Findings from our group and others indicate that female rodents are more reactive to ketamine's antidepressant effects, since they respond to lower doses in antidepressant-predictive behavioral models. Read More
Cancer Cell 2018 Dec;34(6):954-969.e4
Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Center for Molecular Medicine and Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan; Department of Biotechnology, Asia University, Taichung 413, Taiwan. Electronic address:
Multiple mechanisms of resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) have been identified in EGFR-mutant non-small cell lung cancer (NSCLC); however, recurrent resistance to EGFR TKIs due to the heterogeneous mechanisms underlying resistance within a single patient remains a major challenge in the clinic. Here, we report a role of nuclear protein kinase Cδ (PKCδ) as a common axis across multiple known TKI-resistance mechanisms. Specifically, we demonstrate that TKI-inactivated EGFR dimerizes with other membrane receptors implicated in TKI resistance to promote PKCδ nuclear translocation. Read More
Cancer Cell 2018 Dec;34(6):879-891
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia. Electronic address:
Defects in apoptotic cell death can promote cancer and impair responses of malignant cells to anti-cancer therapy. Pro-survival BCL-2 proteins prevent apoptosis by keeping the cell death effectors, BAX and BAK, in check. The BH3-only proteins initiate apoptosis by neutralizing the pro-survival BCL-2 proteins. Read More
Int J Biol Macromol 2018 Dec 8. Epub 2018 Dec 8.
Grigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, Iasi, Romania. Electronic address:
Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method. The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids. Read More
Hum Pathol 2018 Dec 8. Epub 2018 Dec 8.
Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi 832002, China. Electronic address:
Esophageal squamous cell carcinoma (ESCC) is a highly invasive disease with a poor long-term survival rate. Although there has been progress in understanding the pathogenesis of ESCC, there are currently no molecular biomarkers that are used in routine clinical practices to determine prognosis. Therefore, the aim of this study was to determine a small immunohistochemical (IHC) panel that could predict the prognosis of patients with ESCC. Read More
Hum Pathol 2018 Dec 8. Epub 2018 Dec 8.
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China. Electronic address:
Mitogen-activated protein kinase kinase 4 (MKK4) and mitogen-activated protein kinase kinase 7 (MKK7) were shown to regulate biological behavior in many malignancies. In pancreatic ductal adenocarcinoma (PDAC), it remains controversial whether MKK4 and MKK7 have pro-oncogenic or tumor-suppressive activities. Furthermore, their clinicopathologic and prognostic implications are unknown. Read More
Life Sci 2018 Dec 8. Epub 2018 Dec 8.
Department of hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Aims: Acute lymphoblastic leukemia (ALL) is characterized by abnormal proliferation of immature lymphocytes in the bone marrow, peripheral blood, and other tissues. HOXB7 is upregulated in tumors and is related to cell proliferation and cell cycle. However, the role of HOXB7 in ALL progression remains unclear. Read More
J Pharm Sci 2018 Dec 8. Epub 2018 Dec 8.
Faculty of Pharmaceutical Sciences, Sojo University; DDS Research Institute, Ikeda 4-22-Nishi-ku, Kumamoto 860-0082, Japan. Electronic address:
Previously, we reported on the high affinity binding of aripiprazole (ARP), an antipsychotic drug, to human albumin and the role of the chlorine atom of ARP on this binding. In this study, we investigated the binding mode of ARP to human albumin in detail using ARP derivatives and several animal-derived albumins. ARP bound strongly to human and dog albumin. Read More