6,865,351 results match your criteria Protein & Peptide Letters [Journal]

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PTEN-deficient prostate cancer is associated with an immunosuppressive tumor microenvironment mediated by increased expression of IDO1 and infiltrating FoxP3+ T regulatory cells.

Prostate 2019 Apr 18. Epub 2019 Apr 18.

Cancer Biology and Genetics, Queen's Cancer Research Institute, Queen's University, Kingston, Canada.

Background: Accumulating evidence shows that tumor cell-specific genomic changes can influence the cross talk between cancer cells and the surrounding tumor microenvironment (TME). Loss of the PTEN tumor suppressor gene is observed in 20% to 30% of prostate cancers (PCa) when first detected and the rate increases with PCa progression and advanced disease. Recent findings implicate a role for PTEN in cellular type I interferon response and immunosuppression in PCa. Read More

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http://dx.doi.org/10.1002/pros.23808DOI Listing

Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro.

Homeopathy 2019 Apr 18. Epub 2019 Apr 18.

Research Center, Graduating Program of Environmental and Experimental Pathology, Universidade Paulista - UNIP, São Paulo, São Paulo, Brazil.

Introduction:   (), a fungus that acts as an intracellular pathogen, causes a marked neurological syndrome in many host species and is a zoonotic concern. Although no well-established treatment for this syndrome is known, previous successful clinical experience using homeopathic phosphorus has been described in which symptom remission with no mortality occurred in 40/42 animals by means of unknown immunological mechanisms. The latter observation was the main motivation for this study. Read More

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http://dx.doi.org/10.1055/s-0039-1678700DOI Listing

[Determination of the immunoglobulin G supply in the newborn calf].

Tierarztl Prax Ausg G Grosstiere Nutztiere 2019 Apr 18;47(2):97-108. Epub 2019 Apr 18.

Klinik für Geburtshilfe, Gynäkologie und Andrologie der Groß- und Kleintiere der Justus-Liebig-Universität Gießen.

A sufficient supply of colostral antibodies within the first hours of life is crucial for the development and the health status in young calves. It is rational to examine the immunoglobulin uptake of single animals, but particularly on a herd basis, during herd controls and consultations. This enables economical calf rearing in accordance with animal welfare. Read More

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http://dx.doi.org/10.1055/a-0858-4038DOI Listing

Behavioral and Biochemical Features of the Course and Surgical Treatment of Experimental Obstructive Hydrocephalus in Young Rats.

Dev Neurosci 2019 Apr 18:1-10. Epub 2019 Apr 18.

Division of Pediatric Neurosurgery, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

Introduction: Hydrocephalus is a multifactorial disease, affecting the dynamics of cerebrospinal fluid (CSF) and leading to severe neurological impairment in children; in spite of the recent advances in hydrocephalus research, it has many physiopathological aspects that still remain poorly understood, especially after treatment.

Objectives: To analyze the clinical, radiological, histopathological, and biochemical aspects of kaolin-induced hydrocephalus in an experimental model, both in the acute phase and after shunt treatment, by means of behavioral tests, magnetic resonance imaging (MRI) scans, histopathological studies, and level of inflammatory interleukins in the CSF.

Methods: Seven-day-old Wistar rats were used and subdivided into three subgroups: treated hydrocephalic (n = 24), untreated hydrocephalic (n = 17), and controls (n = 5). Read More

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http://dx.doi.org/10.1159/000497433DOI Listing

Prognostic role of GPER/Ezrin in triple negative breast cancer is associated with menopausal status.

Endocr Connect 2019 Apr 1. Epub 2019 Apr 1.

T Wang, Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.

The role of G protein-coupled estrogen receptor 1 (GPER) signaling including promoting Ezrin phosphorylation, which could be also activated by estrogen has not yet been clearly identified in triple negative breast cancer (TNBC). This study is to evaluate the prognostic value of GPER and Ezrin in TNBC patients. Clinico-pathologic features including age, menopausal status, tumor size, nuclear grade, lymph node metastasis, AJCC TNM stage and ER, PR, HER-2 expression were evaluated from 249 TNBC cases. Read More

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https://ec.bioscientifica.com/view/journals/ec/aop/ec-19-016
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http://dx.doi.org/10.1530/EC-19-0164DOI Listing
April 2019
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MADD silencing enhances anti-tumor activity of TRAIL in anaplastic thyroid cancer.

Endocr Relat Cancer 2019 Jun;26(6):551-563

Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois, USA.

ATC is an aggressive disease with limited therapeutic options due to drug resistance. TRAIL is an attractive anti-cancer therapy that can trigger apoptosis in a cancer cell-selective manner. However, TRAIL resistance is a major clinical obstacle for its use as a therapeutic drug. Read More

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https://erc.bioscientifica.com/view/journals/erc/26/6/ERC-18
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http://dx.doi.org/10.1530/ERC-18-0517DOI Listing
June 2019
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Multiple distinct O-Mannosylation pathways in eukaryotes.

Curr Opin Struct Biol 2019 Apr 15;56:171-178. Epub 2019 Apr 15.

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. Electronic address:

Protein O-mannosylation (O-Man), originally discovered in yeast five decades ago, is an important post-translational modification (PTM) conserved from bacteria to humans, but not found in plants or nematodes. Until recently, the homologous family of ER-located protein O-mannosyl transferases (PMT1-7 in yeast; POMT1/POMT2 in humans), were the only known enzymes involved in directing O-Man biosynthesis in eukaryotes. However, recent studies demonstrate the existence of multiple distinct O-Man glycosylation pathways indicating that the genetic and biosynthetic regulation of O-Man in eukaryotes is more complex than previously envisioned. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X183017
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http://dx.doi.org/10.1016/j.sbi.2019.03.003DOI Listing
April 2019
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In silico analysis of different signal peptides for the secretory production of recombinant human keratinocyte growth factor in Escherichia coli.

Comput Biol Chem 2019 Mar 29;80:225-233. Epub 2019 Mar 29.

Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. Electronic address:

Background: The recombinant human truncated Keratinocyte growth factor (Palifermin) is the only FDA approved medicine for the treatment of oral mucositis. The Keratinocyte growth factor is a fairly unstable protein due to its high aggregation propensity and therefore its expression as a secretory protein may results in the production of a protein with more stability, higher solubility, better folding, enhanced biological activity, N-terminal authenticity and simplified downstream processing.

Objective: The aim of this study was in silico evaluation of 31 different secretory signal peptides to determine the best theoretical candidates for the secretory production of recombinant truncated human KGF in E. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2019.03.003DOI Listing

Dietary inflammatory index is positively associated with serum high-sensitivity C-reactive protein in a Korean adult population.

Nutrition 2018 Nov 23;63-64:155-161. Epub 2018 Nov 23.

Cancer Prevention and Control Program, University of South Carolina, Columbia, South Carolina, USA; Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, South Carolina, USA; Connecting Health Innovations LLC, Columbia, South Carolina, USA.

Objective: To our knowledge, only a few studies have explored the relationship between the inflammatory potential of diet and serum inflammatory markers in Korean adults. The likely novel aim of this study was to examine the association between the dietary inflammatory index (DII) and serum high-sensitivity C-reactive protein (hs-CRP) in a Korean adult population.

Methods: A cross-sectional study was conducted using the data set from the Korea National Health and Nutrition Examination Survey (KNHANES) 2015. Read More

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http://dx.doi.org/10.1016/j.nut.2018.11.016DOI Listing
November 2018

Hydrazones as novel epigenetic modulators: Correlation between TET 1 protein inhibition activity and their iron(II) binding ability.

Bioorg Chem 2019 Feb 16;88:102809. Epub 2019 Feb 16.

BIOCEV, First Faculty of Medicine, Charles University, Průmyslová 595, 252 50 Vestec, Czech Republic; Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Technická 5, 166 28 Prague 6, Czech Republic; Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Ke Karlovu 2, 121 08 Prague 2, Czech Republic. Electronic address:

Ten-eleven translocation protein (TET) 1 plays a key role in control of DNA demethylation and thereby of gene expression. Dysregulation of these processes leads to serious pathological states such as oncological and neurodegenerative ones and thus TET 1 targeting is highly requested. Therefore, in this work, we examined the ability of hydrazones (acyl-, aroyl- and heterocyclic hydrazones) to inhibit the TET 1 protein and its mechanism of action. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.02.034DOI Listing
February 2019

Gateway to the Golgi: molecular mechanisms of nucleotide sugar transporters.

Curr Opin Struct Biol 2019 Apr 15;57:127-134. Epub 2019 Apr 15.

Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK. Electronic address:

The Golgi apparatus plays a central role in the secretory pathway as a hub for posttranslational modification, protein sorting and quality control. To date, there is little structural or biochemical information concerning the function of transporters that reside within this organelle. The SLC35 family of nucleotide sugar transporters link the synthesis of activated sugar molecules and sulfate in the cytoplasm, with the luminal transferases that catalyse their attachment to proteins and lipids during glycosylation and sulfation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X183013
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http://dx.doi.org/10.1016/j.sbi.2019.03.019DOI Listing
April 2019
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Plant cell division - defining and finding the sweet spot for cell plate insertion.

Authors:
Sabine Müller

Curr Opin Cell Biol 2019 Apr 15;60:9-18. Epub 2019 Apr 15.

Center for Plant Molecular Biology, Auf der Morgenstelle 32, 72076 Tübingen, Germany. Electronic address:

The plant microtubules form unique arrays using acentrosomal microtubule nucleation pathways, yet utilizing evolutionary conserved centrosomal proteins. In cytokinesis, a multi-component cytoskeletal apparatus, the phragmoplast mediates the biosynthesis of the new cell plate by dynamic centrifugal expansion, a process that demands exquisite coordination of microtubule turnover and endomembrane trafficking. At the same time, the phragmoplast is guided to meet with the parental wall at a cortical site that is predefined before mitotic entry and transiently marked by the preprophase band of microtubules. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09550674193001
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http://dx.doi.org/10.1016/j.ceb.2019.03.006DOI Listing
April 2019
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Folding the genome into mitotic chromosomes.

Curr Opin Cell Biol 2019 Apr 15;60:19-26. Epub 2019 Apr 15.

Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan. Electronic address:

How linear DNA molecules are packaged into compact cylindrical chromosomes in preparation for cell division has remained one of the central outstanding questions in cell biology. Condensin is a highly conserved protein complex that universally determines large-scale DNA geometry during mitotic chromosome assembly. A wide range of recently developed approaches, including super resolution microscopy, single molecule imaging, Hi-C analyses and computational modeling, have profoundly changed how we view mitotic chromosomes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09550674193002
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http://dx.doi.org/10.1016/j.ceb.2019.03.005DOI Listing
April 2019
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Inactivation of NikR from Helicobacter pylori by a bismuth drug.

J Inorg Biochem 2019 Apr 8;196:110685. Epub 2019 Apr 8.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, China, 510275. Electronic address:

The NikR protein is an essential DNA regulator of Helicobacter pylori, a human pathogen, which infects almost half of the world's population. Herein, we comprehensively characterized the interaction of a bismuth drug with Helicobacter pylori NikR. We show that Bi(III) can occupy the high-affinity Ni(II) site of NikR. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2019.03.025DOI Listing

Identification and expression of microRNAs in european eels Anguilla anguilla from two natural sites with different pollution levels.

Environ Pollut 2019 Apr 11;250:274-283. Epub 2019 Apr 11.

Univ. Bordeaux, UMR EPOC CNRS 5805, 33615, Pessac, France.

MicroRNAs (miRNAs) are a class of small non-coding RNA that control multiple biological processes through negative post-transcriptional regulation of gene expression. Recently a role of miRNAs in the response of aquatic organisms to environmental toxicants emerged. Toxicant-induced changes in miRNA expression might then represent novel biomarkers to evaluate the health status of these organisms. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02697491183507
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http://dx.doi.org/10.1016/j.envpol.2019.04.029DOI Listing
April 2019
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Characterization of a Dibenzofuran-degrading strain of Pseudomonas aeruginosa, FA-HZ1.

Environ Pollut 2019 Apr 8;250:262-273. Epub 2019 Apr 8.

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. Electronic address:

Dibenzofuran (DBF) derivatives have caused serious environmental problems, especially those produced by paper pulp bleaching and incineration processes. Prominent for its resilient mutagenicity and toxicity, DBF poses a major challenge to human health. In the present study, a new strain of Pseudomonas aeruginosa, FA-HZ1, with high DBF-degrading activity was isolated and identified. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02697491193032
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http://dx.doi.org/10.1016/j.envpol.2019.04.026DOI Listing
April 2019
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Effects of perfluorooctanoic acid on stem Leydig cell functions in the rat.

Environ Pollut 2019 Apr 9;250:206-215. Epub 2019 Apr 9.

Department of Pediatrics, Peking University First Hospital, No.1 Xi'an Men Street, West District, Beijing, 100034, China; Department of Pediatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China. Electronic address:

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic (PFOS) are two perfluorinated chemical products widely existing in the environment. Evidence suggested that PFOA might relate to male reproductive dysfunction in rats and humans. PFOA exposure inhibited the function of Leydig cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02697491193015
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http://dx.doi.org/10.1016/j.envpol.2019.03.120DOI Listing
April 2019
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SA and AM symbiosis modulate antioxidant defense mechanisms and asada pathway in chickpea genotypes under salt stress.

Ecotoxicol Environ Saf 2019 Apr 15;178:66-78. Epub 2019 Apr 15.

Department of Botany, Panjab University, Chandigarh, 160014, India. Electronic address:

Salt stress disturbs redox homeostasis by perturbing equilibrium between generation and removal of reactive oxygen species (ROS), which alters the normal metabolism of plants through membrane damage, lipid peroxidation and denaturation of proteins. Salicylic acid (SA) seed priming and arbuscular mycorrhizal (AM) fungi impart salt tolerance in legumes by maintaining redox balance. The present investigation focused on the relative and combined applications of SA and Rhizoglomus intraradices in scavenging ROS in Cicer arietinum L. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.025DOI Listing

Protective effects of tribulus terrestris extract and angiotensin blockers on testis steroidogenesis in copper overloaded rats.

Ecotoxicol Environ Saf 2019 Apr 15;178:113-122. Epub 2019 Apr 15.

Department of Biochemistry, Faculty of Pharmacy, Zagazig University, 44519, Zagazig, Sharkia Gov., Egypt. Electronic address:

The rational of the current study was to assess whether Tribulus terrestris extract (TTE) could alleviate long-term copper (Cu) overload-induced testicular dysfunction compared to enalapril and losartan. Rats were administered either vehicle (control group, n = 10) or copper sulfate pentahydrate (CuSO·5HO, 200 mg/kg, p.o) for 90 days (n = 40). Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.012DOI Listing

Peptide/receptor-like kinase-mediated signaling involved in male-female interactions.

Curr Opin Plant Biol 2019 Apr 15;51:7-14. Epub 2019 Apr 15.

State Key Laboratory for Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences at College of Life Sciences, Peking University, Beijing 100871, People's Republic of China; The National Plant Gene Research Center (Beijing), Beijing 100101, People's Republic of China. Electronic address:

In flowering plants, extensive male-female interactions during pollen germination on the stigma, pollen tube growth and guidance in the transmitting tract, and pollen tube reception by the female gametophyte are required for successful double fertilization in which various signaling cascades are involved. Peptide/receptor-like kinase-mediated signaling has been found playing important roles in these male-female interactions. Here, we mainly summarized the progress made on the regulatory roles of peptide/receptor-like kinase-mediated signaling pathways in four critical stages during reproduction in higher plants. Read More

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http://dx.doi.org/10.1016/j.pbi.2019.03.004DOI Listing

Molecular cloning and mRNA expression of IFIT5 in tissues of ducklings infected with virulent duck hepatitis A virus type 3.

Res Vet Sci 2019 Apr 8;124:256-262. Epub 2019 Apr 8.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, PR China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of proteins strongly induced downstream of type I interferon signaling. The function of IFITs has been investigated extensively in mammals. IFIT5 is the sole protein in this family found in birds and little information is available about the function of avian IFIT5. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00345288183132
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http://dx.doi.org/10.1016/j.rvsc.2019.04.003DOI Listing
April 2019
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Superparamagnetic iron oxide nanoparticles of curcumin enhance gemcitabine therapeutic response in pancreatic cancer.

Biomaterials 2019 Apr 8;208:83-97. Epub 2019 Apr 8.

Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, USA. Electronic address:

Pancreatic cancer is a complex disease accounting for fibrotic tumors and an aggressive phenotype. Gemcitabine (GEM) is used as a standard therapy, which develops chemoresistance leading to poor patient outcome. We have recently developed a superparamagnetic iron oxide nanoparticle (SPION) formulation of curcumin (SP-CUR), which is a nontoxic, bioactive anti-inflammatory/anti-cancer agent for its enhanced delivery in tumors. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.005DOI Listing

The effect of salts in aqueous media on the formation of the BSA corona on SiO nanoparticles.

Colloids Surf B Biointerfaces 2019 Apr 6;179:374-381. Epub 2019 Apr 6.

Department of Chemical and Biochemical Engineering, 4133 Seamans Center for the Engineering Arts and Sciences, The University of Iowa, Iowa City, IA, USA. Electronic address:

Protein-nanoparticle interactions are garnering attention due to their potential impacts on human health and environmental contamination. The colloidal properties of nanoparticles (NPs) in aqueous media may differ in the presence of natural materials such as salts and proteins. In this study, the interactions between bovine serum albumin (BSA) and fumed hydrophilic silicon dioxide (SiO) NPs were studied in aqueous solutions under variable pH or ion composition. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.012DOI Listing

Autophagy represses fascaplysin-induced apoptosis and angiogenesis inhibition via ROS and p8 in vascular endothelia cells.

Biomed Pharmacother 2019 Apr 15;114:108866. Epub 2019 Apr 15.

School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.

Fascaplysin is a natural product isolated from marine sponges that exhibits broad anticancer activity. Previous studies revealed that fascaplysin-induced apoptosis and angiogenesis inhibition in vascular endothelial cells contributed to its anticancer activity. Accumulating evidence indicates that autophagy plays a significant role in mediating the function of vascular endothelial cells (VECs) and the response to cancer therapy. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108866DOI Listing

Role of DNA hypomethylation in lateral habenular nucleus in the development of depressive-like behavior in rats.

J Affect Disord 2019 Mar 22;252:373-381. Epub 2019 Mar 22.

Department of Physiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China. Electronic address:

Background: Lateral habenula nucleus (LHb) has recently been noted for its role in stress-induced depressive disorder. Yet little is known about the mechanisms by which external stimuli or depression induces pathological alteration in the LHb.

Methods: Chronic unpredictable mild stress (CUMS) was employed to model depressive-like behaviors in adult rats. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650327183242
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http://dx.doi.org/10.1016/j.jad.2019.03.062DOI Listing
March 2019
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Altered dynamics may drift pathological fibrillization in membraneless organelles.

Biochim Biophys Acta Proteins Proteom 2019 Apr 15. Epub 2019 Apr 15.

MTA-DE Laboratory of Protein Dynamics, University of Debrecen, Hungary. Electronic address:

Protein phase transition can generate non-membrane bound cellular compartments, which can convert from liquid-like to solid-like states. While the molecular driving forces of phase separation have been largely understood, much less is known about the mechanisms of material-state conversion. We apply a recently developed algorithm to describe the weak interaction network of multivalent motifs, and simulate the effect of pathological mutations. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.005DOI Listing

Functional characterization of chloroplast transit peptide in the small subunit of Rubisco in maize.

J Plant Physiol 2019 Apr 10;237:12-20. Epub 2019 Apr 10.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, JiangSu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, No.1 Weigang, Nanjing, Jiangsu, 210095, China. Electronic address:

Functions of domains or motifs, which are encoded by the transit peptide (TP) of the precursor of the small subunit of Rubisco (prSSU), have been investigated intensively in dicots. Functional characterization of the prSSU TP, however, is still understudied in maize. In this study, we found that the TP of maize prSSU1 did not function fully in chloroplast targeting in Arabidopsis or vice versa, indicating the divergent function of TPs in chloroplast targeting between maize and Arabidopsis. Read More

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http://dx.doi.org/10.1016/j.jplph.2019.04.001DOI Listing

Overexpression of S-nitrosoglutathione reductase alleviated iron-deficiency stress by regulating iron distribution and redox homeostasis.

J Plant Physiol 2019 Apr 1;237:1-11. Epub 2019 Apr 1.

State Key Laboratory of Crop Biology, Ministry of Agriculture Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Huang-Huai Region, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, PR China. Electronic address:

Iron (Fe) is an essential micronutrient element for plant growth. The S-nitrosoglutathione reductase (GSNOR) gene's functions under Fe-deficiency conditions are not well understood. Here, GSNOR expression was induced by Fe deficiency in tomato (Solanum lycopersicum L. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01761617183032
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http://dx.doi.org/10.1016/j.jplph.2019.03.007DOI Listing
April 2019
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The olfactory bulb proteotype differs across frontotemporal dementia spectrum.

J Proteomics 2019 Apr 15. Epub 2019 Apr 15.

Clinical Neuroproteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea, 3, 31008 Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain, Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Mild olfactory dysfunction has been observed in frontotemporal dementias (FTD). However, the underlying molecular mechanisms associated to this deficit are poorly understood. We applied quantitative proteomics to analyze pathological effects on the olfactory bulb (OB) from progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration (FTLD-TDP43) subjects respect to elderly non-FTD group. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.011DOI Listing
April 2019
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Development and pharmacokinetics of a combination vaginal ring for sustained release of dapivirine and the protein microbicide 5P12-RANTES.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. Electronic address:

The past fifteen years have witnessed a resurgence of interest in vaginal ring technologies for drug delivery applications, mostly driven by the impetus for development of vaginally-administered antiretroviral microbicides to help reduce the high acquisition rates for human immunodeficiency virus (HIV) among Sub-Saharan African women. Currently, the lead candidate microbicide is a 28-day silicone elastomer vaginal ring releasing dapivirine (Ring-004), an experimental non-nucleoside reverse transcriptase inhibitor. The ring was tested in two pivotal Phase III clinical studies in 2016 and is currently undergoing review by the European Medicines Agency. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.040DOI Listing

Gold nanoparticles loaded with Cullin-5 DNA increase sensitivity to 17-AAG in Cullin-5 Deficient Breast Cancer Cells.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Department of Biological Sciences, Towson University, Towson, MD, United States. Electronic address:

HSP90 inhibitors have the potential to treat many types of cancer due to the dependence of tumor cells on HSP90 for cell growth and proliferation. The Cullin-5 (Cul5) E3 ubiquitin ligase is required for HSP90 inhibitors to induce client protein degradation and subsequent cell death. Cul5 is expressed at low levels in breast cancer cells compared to patient matched controls. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.022DOI Listing

The Formation and Effect of Mannitol Hemihydrate on the Stability of Monoclonal Antibody in the Lyophilized state.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Lek d.d., Kolodvorska 27, 1234 Mengeš, Slovenia.

Crystalline bulking agent in lyophilized biopharmaceutical formulations provides an elegant lyophilized cake structure and allows aggressive primary drying conditions. The interplay between amorphous and crystalline state of excipients heavily influence the stability of lyophilized biological products and should be carefully evaluated in the formulation and process development phase. This study focuses on: (1) elucidating the influence of formulation and lyophilization process variables on the formation of different states of mannitol and (2) its impact on model monoclonal antibody stability when compared to sucrose. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.044DOI Listing
April 2019
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A JAK-STAT pathway target gene encoding a single WAP domain (SWD)-containing protein from Litopenaeus vannamei.

Fish Shellfish Immunol 2019 Apr 15. Epub 2019 Apr 15.

MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), Guangzhou, PR China. Electronic address:

In shrimp, the JAK-STAT pathway is essentially implicated in both antiviral and antibacterial responses. However, few regulatory target genes of the JAK-STAT pathway in shrimp have been reported so far. In this study, a novel single WAP domain-containing peptide (LvSWD4) was identified from Pacific white shrimp Litopenaeus vannamei. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.046DOI Listing

Haematological and intestinal health parameters of rainbow trout are influenced by dietary live yeast and increased water temperature.

Fish Shellfish Immunol 2019 Apr 15. Epub 2019 Apr 15.

Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Box 7024, 750 07 Uppsala, Sweden. Electronic address:

Live yeast may be a sustainable protein source in salmonid diets while exhibiting a probiotic effect to counteract environmental stressors, such as increased water temperature that is being exacerbated by climate change. The objective of this study was to evaluate the effects of feeding a high dietary inclusion of live yeast and increased water temperature on growth, haematological and intestinal physiology of rainbow trout. For six weeks, 129 g fish in 16 tanks (n = 4) were fed either a diet based on fishmeal or based on live yeast (214 g kg of diet or 7. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193028
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http://dx.doi.org/10.1016/j.fsi.2019.04.047DOI Listing
April 2019
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Adverse and hormetic effects in rats exposed for 12 months to low dose mixture of 13 chemicals: RLRS part III.

Toxicol Lett 2019 Apr 15. Epub 2019 Apr 15.

Center of Toxicology Science & Research, Medical School, University of Crete, Heraklion, Crete, Greece; Department of Analytical and Forensic Medical Toxicology, Sechenov University, Moscow 119991, Russian Federation. Electronic address:

The aim of the current study was to evaluate the effects of a mixture of thirteen common chemicals on rats, after a one-year exposure to doses around the acceptable daily intake (ADIs), using blood and urinary tests. The influence of low doses of the mixture on weight gain, water consumption, feed consumption and feed efficiency, biochemistry parameters, haematological parameters, blood lymphocytes subsets, serum inflammation profile and urine parameters was evaluated. Our mixture caused a moderate monotonic increase of the males' appetite and a non-monotonic increase of anabolism and a monotonic increase of appetite for the females. Read More

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http://dx.doi.org/10.1016/j.toxlet.2019.04.005DOI Listing

Genome-wide identification, phylogeny and function analysis of GRAS gene family in Dendrobium catenatum (Orchidaceae).

Gene 2019 Apr 15. Epub 2019 Apr 15.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China. Electronic address:

Background: In recent years, the molecular mechanism of plant growth and development has been reported in detail. GRAS genes, a plant-specific family of transcription factor, play critical roles in the process. GRAS transcription factors are associated with axillary shoot meristem formation, radial root patterning, phytohormones (gibberellins) signal transduction, light signaling, and abiotic or biotic stress. Read More

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http://dx.doi.org/10.1016/j.gene.2019.04.038DOI Listing

Sex-Specific Neurogenic Deficits and Neurocognitive Disorders in Middle-Aged HIV-1 Tg26 Transgenic Mice.

Brain Behav Immun 2019 Apr 15. Epub 2019 Apr 15.

Center for Metabolic Disease Research, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, United States; Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, 3500 N Broad Street, Philadelphia, PA 19140, United States. Electronic address:

Varying degrees of cognitive deficits affect over half of all HIV-1 infected patients. Because of antiretroviral treatment (ART) regimens, the HIV-1 patient population is increasing in age. Very few epidemiological studies have focused on sex-specific differences in HIV-1-associated neurocognitive disorders (HAND). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08891591183060
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http://dx.doi.org/10.1016/j.bbi.2019.04.029DOI Listing
April 2019
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Evaluation of the schistosomicidal, antioxidant and anti-inflammatory activities of the ethyl acetate fraction from Ozoroa pulcherrima Schweinf. Roots on Schistosoma mansoni-induced liver pathology in mice and its phytochemical characterization.

J Ethnopharmacol 2019 Apr 15:111883. Epub 2019 Apr 15.

Laboratory of Biology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon; Centre for Schistosomiasis and Parasitology, P.O. Box 7244, Yaoundé, Cameroon. Electronic address:

Ethnopharmacological Relevance: Ozoroa pulcherrima Schweinf. (syn.: Heeria pulcherrrima Schweinf. Read More

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http://dx.doi.org/10.1016/j.jep.2019.111883DOI Listing

Anti-atherogenic effects of CD36-targeted epigallocatechin gallate-loaded nanoparticles.

J Control Release 2019 Apr 15. Epub 2019 Apr 15.

Department of Nutritional Sciences, Texas Tech University, Lubbock, TX 79409, USA. Electronic address:

Intimal macrophages play a critical role in atherosclerotic lesion initiation and progression by taking up oxidized low-density lipoprotein (oxLDL) and promoting inflammatory process. 1-(Palmitoyl)-2-(5-keto-6-octene-dioyl) phosphatidylcholine (KOdiA-PC), a major type of oxidized phosphatidylcholines (PC) found on oxLDL, has a high binding affinity to the macrophage scavenger receptor CD36 and participates in CD36-mediated recognition and uptake of oxLDL by intimal macrophages. We successfully synthesized epigallocatechin gallate (EGCG)-loaded nanoparticles (Enano), which were composed of EGCG, PC, (+) alpha-tocopherol acetate, and surfactant. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.018DOI Listing

MPL nano-liposomal vaccine containing P5 HER2/neu-derived peptide pulsed PADRE as an effective vaccine in a mice TUBO model of breast cancer.

J Control Release 2019 Apr 15. Epub 2019 Apr 15.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Liposomal peptide-based vaccines can potentially suppress cancer cells proliferation in the host. To enhance the effectiveness of vaccination against cancer, additional strategies should also be employed. One strategy to promote peptide-based vaccine efficacy and induce powerful immune responses, is simultaneous activation of CD4 and CD8 T cells. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.019DOI Listing

Differential Expression of HMGA1 and HMGA2 in pituitary neuroendocrine tumors.

Mol Cell Endocrinol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Experimental Endocrinology - LEEx, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil; Graduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Brazil; Graduate Program in Pharmacology and Medicinal Chemistry, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil. Electronic address:

Defining biomarkers for invasive pituitary neuroendocrine tumors (PitNETs) is highly desirable. The high mobility group A (HMGA) proteins are among the most widely expressed cancer-associated proteins. Indeed, their overexpression is a frequent feature of human malignancies, including PitNETs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193011
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http://dx.doi.org/10.1016/j.mce.2019.04.010DOI Listing
April 2019
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Fatty acid binding protein 4 (FABP4) as a potential biomarker reflecting myocardial lipid storage in type 2 diabetes.

Metabolism 2019 Apr 15. Epub 2019 Apr 15.

Vascular Medicine and Metabolism Unit, Research Unit on Lipids and Atherosclerosis, "Sant Joan" University Hospital, Universitat Rovira i Virgili, Institut de Investigació Sanitaria Pere Virgili (IISPV), Reus, Spain; Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Institute of Health Carlos III, Madrid, Spain. Electronic address:

Objective: Fatty acid binding protein 4 (FABP4) is an intracellular lipid chaperone involved in the crosstalk between adipose and peripheral tissues, and it contributes to widespread insulin resistance in cells, including cardiac cells. However, the role of this adipokine in regulating cardiac metabolism and myocardial neutral lipid content in patients with type 2 diabetes has not been elucidated.

Methods: The impact of circulating FABP4 on the cardiac neutral lipid content were measured by proton magnetic resonance spectroscopy (H-MRS) in patients with type 2 diabetes. Read More

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http://dx.doi.org/10.1016/j.metabol.2019.04.007DOI Listing

Methylation Tolerance-based Functional Assay to Assess Variants of Unknown Significance in the MLH1 and MSH2 Genes and Identify Patients With Lynch Syndrome.

Gastroenterology 2019 Apr 15. Epub 2019 Apr 15.

Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, F-75012 Paris, France; SIRIC CURAMUS, Equipe labellisée par la Ligue Nationale contre le Cancer. Electronic address:

Background & Aims: Approximately 75% of patients with suspected Lynch syndrome carry variants in MLH1 or MSH2-proteins encoded by these genes are required for DNA mismatch repair (MMR). However, 30% of these are variants of unknown significance (VUS). A assay that measures cell response to the cytotoxic effects of a methylating agent can determine the effects of VUS in MMR genes and identify patients with constitutional mismatch repair-deficiency syndrome. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.03.071DOI Listing

Oxidative stress-induced HMGB1 release from melanocytes: a paracrine mechanism underlying the cutaneous inflammation in vitiligo.

J Invest Dermatol 2019 Apr 15. Epub 2019 Apr 15.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi'an, shaanxi, 710032, China. Electronic address:

Vitiligo is a cutaneous depigmentation disorder caused by the destruction of epidermal melanocytes. The generation and the skin infiltration of autoreactive CD8 cytotoxic T cells triggered by oxidative stress play a critical role in vitiligo. High-mobility group protein B1 (HMGB1) is a classic damage-associated molecular pattern (DAMP) molecule with strong proinflammatory effects in inflammatory reactions. Read More

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http://dx.doi.org/10.1016/j.jid.2019.03.1148DOI Listing

Downregulation of ATR decreases the responsiveness of BK channels to angiotensin II in patients with hypertension.

J Mol Cell Cardiol 2019 Apr 15. Epub 2019 Apr 15.

Key Lab of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Lab of Sichuan Province, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China. Electronic address:

Angiotensin II (Ang II) modulates blood pressure via Ang II type 1 receptor (ATR) and type 2 receptor (ATR). The activation of ATR relaxes vascular tone through opening large-conductance Ca-activated potassium (BK) channels in vascular smooth muscle cells (SMCs). In the present study, we studied the role of the ATR-BK pathway in patients with hypertension. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2019.04.013DOI Listing

The assembly and evaluation of antisense oligonucleotides applied in exon skipping for titin-based mutations in dilated cardiomyopathy.

J Mol Cell Cardiol 2019 Apr 15. Epub 2019 Apr 15.

Department of Cardiology and Cardiovascular Diseases, Eberhard Karls University, Tübingen, Germany; Department of Cardiology, RWTH Aachen, Pauwelsstr. 30, Aachen, Germany. Electronic address:

The leading cause of genetic dilated cardiomyopathy (DCM) is due to mutations in the TTN gene, impacting approximately 15-20% of familial and 18% of sporadic DCM cases. Currently, there is potential for a personalized RNA-based therapeutic approach in titin-based DCM, utilizing antisense oligonucleotide (AON) mediated exon-skipping, which attempts to reframe mutated titin transcripts, resulting in shortened, functional protein. However, the TTN gene is massive with 363 exons; each newly identified TTN exon mutation provides a challenge to address when considering the potential application of AON mediated exon skipping. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00222828193003
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http://dx.doi.org/10.1016/j.yjmcc.2019.04.014DOI Listing
April 2019
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Activation of the PP2A catalytic subunit by ivabradine attenuates the development of diabetic cardiomyopathy.

J Mol Cell Cardiol 2019 Apr 15. Epub 2019 Apr 15.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China. Electronic address:

Hyperglycemia-induced apoptosis plays a critical role in the pathogenesis of diabetic cardiomyopathy (DCM). Our previous study demonstrated that ivabradine, a selective I current antagonist, significantly attenuated myocardial apoptosis in diabetic mice, but the underlying mechanisms remained unknown. This study investigated the underlying mechanisms by which ivabradine exerts anti-apoptotic effects in experimental DCM. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2019.04.011DOI Listing

Improvement of soluble expression of GM-CSF in the cytoplasm of Escherichia coli using chemical and molecular chaperones.

Protein Expr Purif 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address:

The most common approaches to improve soluble expression of heterologous proteins are applications of molecular chaperones such as DnaK, DnaJ, GrpE, GroEL and GroES. The aim of present study was to enhance soluble expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Escherichia coli by different approaches including modification of cultivation and induction conditions, and thermally, genetically and chemically enhancement of expression of cellular chaperones. To genetically enhance amount of molecular chaperones, co-expression of pET28-GM-CSF and pKJE7 plasmids was performed. Read More

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http://dx.doi.org/10.1016/j.pep.2019.04.002DOI Listing

The ELAV family of RNA-binding proteins in synaptic plasticity and long-term memory.

Neurobiol Learn Mem 2019 Apr 15. Epub 2019 Apr 15.

Center for Neural Science, New York University, New York, NY 10003, USA. Electronic address:

The mechanisms of de novo gene expression and translation of specific gene transcripts have long been known to support long-lasting changes in synaptic plasticity and behavioral long-term memory. In recent years, it has become increasingly apparent that gene expression is heavily regulated not only on the level of transcription, but also through post-transcriptional gene regulation, which governs the subcellular localization, stability, and likelihood of translation of mRNAs. Specific families of RNA-binding proteins (RBPs) bind transcripts which contain AU-rich elements (AREs) within their 3' UTR and thereby govern their downstream fate. Read More

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http://dx.doi.org/10.1016/j.nlm.2019.04.007DOI Listing

Glyphaea brevis - In vitro antioxidant and in silico biological activity of major constituents and molecular docking analyses.

Toxicol In Vitro 2019 Apr 15. Epub 2019 Apr 15.

Phytomedicine, Biochemical Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Sciences, PMB 704, The Federal University of Technology, Akure, Nigeria.

Previous studies have revealed that leaf extracts of Glyphaea brevis possess antioxidant activity but the bioactivity and mechanisms of action of its major constituents remain unknown. This study evaluated in vitro antioxidant and free radical scavenging activities of Glyphaea brevis twigs and leaves, and probable toxicity profile, pharmacological activities and mechanisms of action of major phytoconstituents in silico. Phytochemical screening detected saponins, tannins, steroids, anthraquinones, flavonoids, terpenoids and phenolics in the extracts. Read More

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http://dx.doi.org/10.1016/j.tiv.2019.04.013DOI Listing
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