6,768,718 results match your criteria Protein & Peptide Letters [Journal]

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Apolipoprotein CIII and diabetes. Is there a link?

Diabetes Metab Res Rev 2018 Dec 17:e3118. Epub 2018 Dec 17.

Department of Internal Medicine, School of Medicine, University of Ioannina, Ioannina, Greece.

Apolipoprotein CIII (ApoCIII), a small protein that resides on the surface of lipoprotein particles, is a key regulator of triglyceride metabolism. The inhibition of lipoprotein lipase (LPL), the increased assembly and secretion of very low-density lipoproteins (VLDL) and the decreased reuptake of triglyceride-rich lipoproteins (TRLs) by the liver are mechanisms associating elevated serum ApoCIII levels and hypertriglyceridemia. ApoCIII concentration is high in individuals with diabetes mellitus indicating a possible positive correlation with impairment of glucose metabolism. Read More

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http://dx.doi.org/10.1002/dmrr.3118DOI Listing
December 2018

The Role of Toll-Like Receptors in the Production of Cytokines by Human Lung Macrophages.

J Innate Immun 2018 Dec 17:1-11. Epub 2018 Dec 17.

Département des Maladies Respiratoires, Hôpital Foch, Suresnes, France.

Background: The Toll-like receptor (TLR) family is involved in the recognition of and response to microbial infections. These receptors are expressed in leukocytes. TLR stimulation induces the production of proinflammatory cytokines and chemokines. Read More

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http://dx.doi.org/10.1159/000494463DOI Listing
December 2018

Primary Human Neutrophils Exhibit a Unique HIV-Directed Antibody-Dependent Phagocytosis Profile.

J Innate Immun 2018 Dec 17:1-10. Epub 2018 Dec 17.

The only clinical HIV vaccine trial to demonstrate efficacy, RV144, correlated protection with the antibodies (Abs) mediating function via the "constant" immunoglobulin region, the crystallizable fragment (Fc). These data have supported a focus on the induction of Abs by vaccines that trigger antiviral activities by relevant leukocytes via Fc receptors (FcRs). Neutrophils are phagocytes that comprise > 50% of leukocytes and display unique FcRs. Read More

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http://dx.doi.org/10.1159/000494371DOI Listing
December 2018
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miR-505-3p is a repressor of the puberty onset in female mice.

J Endocrinol 2018 Dec 1. Epub 2018 Dec 1.

J Xiao, The College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.

Puberty onset is a complex trait regulated by multiple genetic and environmental factors. In this study, we narrowed a puberty related QTL region down to a 1.7 Mb region on chromosome X in female mice and inferred miR-505-3p as the functional gene. Read More

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http://dx.doi.org/10.1530/JOE-18-0533DOI Listing
December 2018
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Regulation of inositol 1,4,5-trisphosphate-induced Ca release from the endoplasmic reticulum by AMP-activated kinase modulators.

Cell Calcium 2018 Dec 11;77:68-76. Epub 2018 Dec 11.

Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:

The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More

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http://dx.doi.org/10.1016/j.ceca.2018.12.004DOI Listing
December 2018

Improved ELISA for tumor marker detection using electro-readout-mode based on label triggered degradation of methylene blue.

Biosens Bioelectron 2018 Dec 7;126:800-805. Epub 2018 Dec 7.

Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address:

Enzyme-linked immunosorbent assay (ELISA), a gold-standard method for protein detection, has been widely utilized in disease diagnosis. Nevertheless, the method is constrained by its dependence on a colorimetric readout that, due to its relatively low sensitivity, prevents its use in tumor marker detection. Here we demonstrate an ELISA that incorporates an electro-readout mode in place of a colorimetric readout to achieve ultrasensitive and convenient tumor marker detection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09565663183093
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http://dx.doi.org/10.1016/j.bios.2018.11.038DOI Listing
December 2018
1 Read

Dipeptidyl peptidase-4 inhibition protects the liver of insulin-resistant female rats against triglyceride accumulation by suppressing uric acid.

Biomed Pharmacother 2018 Dec 14;110:869-877. Epub 2018 Dec 14.

HOPE Cardiometabolic Research Team, Department of Physiology, College of Health Sciences, University of Ilorin, Nigeria. Electronic address:

Dipeptidyl peptidase-4 (DPP-4) inhibition has been shown to exert beneficial effects against insulin resistance (IR) and type 2 diabetes. Combined oral contraceptive (COC) treatment is associated with impaired glucose and lipid metabolism but the mechanisms are elusive. We therefore, hypothesized that DPP-4 inhibition ameliorates COC-induced glucose dysregulation and hepatic triglyceride (TG) accumulation through adenosine deaminase (ADA) /xanthine oxidase (XO) /uric acid-dependent pathway. Read More

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http://dx.doi.org/10.1016/j.biopha.2018.12.036DOI Listing
December 2018

CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.

Biomed Pharmacother 2018 Dec 14;110:834-843. Epub 2018 Dec 14.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-Inflammatory and Immune Medicine (Anhui Medical University), Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Hefei, 230032, China. Electronic address:

Objective: CP-25 attenuates arthritis progression in animal models by inhibiting G protein-coupled receptor kinase 2 (GRK2) membrane expression. This study compared groups treated with high-dose methotrexate (MTX)/leflunomide (LEF) and CP-25 combined with low-dose MTX/LEF in an adjuvant-induced arthritis (AA) rat model and investigated possible mechanisms.

Methods: AA was induced in rats via complete Freund's adjuvant. Read More

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http://dx.doi.org/10.1016/j.biopha.2018.12.040DOI Listing
December 2018

Unravel a neuroactive sHA sulfation pattern with neurogenesis activity by a library of defined oligosaccharides.

Eur J Med Chem 2018 Dec 4;163:583-596. Epub 2018 Dec 4.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, 100191, Haidian District, Beijing, China. Electronic address:

Sulfated hyaluronic acid (sHA) is chemically synthetic mimetic of glycosaminoglycan (GAG) presenting promising biological functions. Specific sulfation pattern, termed as sulfation code plays critical roles in regulating the binding mode between GAG and proteins. As a structural analogue of chondroitin sulfate (CS), sHA bears much higher molecular weight and is nearly free of other proteoglycan contaminants. Read More

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http://dx.doi.org/10.1016/j.ejmech.2018.12.004DOI Listing
December 2018

Identification of novel PI3Kδ inhibitors by docking, ADMET prediction and molecular dynamics simulations.

Comput Biol Chem 2018 Dec 7;78:190-204. Epub 2018 Dec 7.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address:

Background: Phosphoinositide-3-kinase Delta (PI3Kδ) plays a key role in B-cell signal transduction and inhibition of PI3Kδ is confirmed to have clinical benefit in certain types of activation of B-cell malignancies. Virtual screening techniques have been used to discover new molecules for developing novel PI3Kδ inhibitors with little side effects.

Method: Computer aided drug design method were used to rapidly screen optimal PI3Kδ inhibitors from the Asinex database. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2018.12.002DOI Listing
December 2018

In utero exposure to bisphenol A disrupts fetal testis development in rats.

Environ Pollut 2018 Dec 7;246:217-224. Epub 2018 Dec 7.

Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou, Zhejiang, 325027, China. Electronic address:

Bisphenol A (BPA) is widely used in consumer products and is a potential endocrine disruptor linked with abnormal development of male reproductive tract. However, its action and its effects on the pathways in the development of male gonad are still unclear. Here we report that effects of BPA exposure during gestation on male gonad development. Read More

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http://dx.doi.org/10.1016/j.envpol.2018.12.006DOI Listing
December 2018
4.143 Impact Factor

Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane.

iScience 2018 Dec 5;10:234-244. Epub 2018 Dec 5.

Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; MRC Centre for Neurodevelopmental Disorders, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK. Electronic address:

Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S25890042183023
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http://dx.doi.org/10.1016/j.isci.2018.12.001DOI Listing
December 2018
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Protein biochips based on macroporous polymer supports: Material properties and analytical potential.

J Pharm Biomed Anal 2018 Dec 10;165:242-250. Epub 2018 Dec 10.

Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, Petrodvorez, 198584, St. Petersburg, Russia; Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004, St. Petersburg, Russia.

A series of rigid macroporous polymer layers differed by hydrophobic-hydrophilic properties was synthesized in situ in preliminary fabricated wells and applied as the platforms for protein biochips. Scanning electron microscopy, etalon porosimetry and BET analysis were used for materials characterization. The comparison of analytical efficiency of the developed platforms allowed for the choice of the most optimal polymer, as well as the evaluation of impact of material porous properties. Read More

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http://dx.doi.org/10.1016/j.jpba.2018.12.012DOI Listing
December 2018

Benzo(a)pyrene inhibits the accumulation and toxicity of cadmium in subcellular fractions of Eisenia fetida.

Chemosphere 2018 Dec 11;219:740-747. Epub 2018 Dec 11.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, PR China. Electronic address:

Cadmium (Cd) and benzo [a]pyrene (BaP) often co-occur in the environment, and the critical body residue of organisms is used as an indicator of the toxic effects of contaminants. However, little is known about their distributions and toxicities when pollution of Cd and BaP are combined. Semi-static solution culture experiment was used to study the impacts of BaP on the subcellular distribution of the toxic metal Cd in the earthworm Eisenia fetida. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2018.12.083DOI Listing
December 2018

Immediate Hypersensitivity to Polyethylene Glycols and Polysorbates: More Common Than We Have Recognized.

J Allergy Clin Immunol Pract 2018 Dec 14. Epub 2018 Dec 14.

Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Background: The most common immediate hypersensitivity to macrogols is associated with PEG 3350, however the epidemiology, mechanisms and cross-reactivity are poorly understood. Thousands of medications contain either PEGs or structurally similar polysorbates.

Objective: Our objective was to better understand the mechanism, cross-reactivity and scope of PEG hypersensitivity. Read More

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http://dx.doi.org/10.1016/j.jaip.2018.12.003DOI Listing
December 2018
2 Reads

Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address:

Myeloid-derived suppressor cells (MDSCs) play an immunosuppressive role in the pathogenesis of inflammatory diseases. CD180, a TLR-like protein, can regulate the proliferation and activation of immune cells. However, the roles of CD180 in regulating the accumulation and function of MDSCs have not been investigated. Read More

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http://dx.doi.org/10.1016/j.bbadis.2018.12.013DOI Listing
December 2018

Isolation of the first New Delhi metallo-ß-lactamase-1 (NDM-1) producing, colistin resistant, Klebsiella pneumoniae sequence type ST15, from a digestive carrier in Albania, May 2018.

J Glob Antimicrob Resist 2018 Dec 14. Epub 2018 Dec 14.

Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Objectives: Carbapenemases represent a public health threat as they can spread through horizontal gene transfer and cause outbreaks. NDM-1 is a metallo-ß-lactamase that has spread rapidly in the last decade causing worldwide alarm. We aimed to describe the first isolate of NDM-1 producing and XDR Klebsiella pneumoniae in Albania, its clinical context and genetic characterization. Read More

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http://dx.doi.org/10.1016/j.jgar.2018.12.002DOI Listing
December 2018

RGD-modified PEGylated paclitaxel nanocrystals with enhanced stability and tumor-targeting capability.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmaceutics, School of Pharmacy, Fudan University & Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai 201203, China.

Nanocrystals has been constructed for insoluble drugs as a novel type of nanoscale drug delivery systems with high drug loading. How to prepare nanocrystals with good stability and tumor targeting capability is still challenging. This study was to modify paclitaxel nanocrystals with polyethylene glycol (PEG) for stabilization and RGD peptide for tumor targeting. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2018.12.023DOI Listing
December 2018

Understanding the transition from water to land: Insights from multi-omic analyses of the perivitelline fluid of apple snail eggs.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Institute of Research and Continuing Education, Shenzhen, China. Electronic address:

Most gastropods deposit their eggs either on land or underwater, but the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919183044
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http://dx.doi.org/10.1016/j.jprot.2018.12.014DOI Listing
December 2018
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Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:

Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.010DOI Listing
December 2018

Identification of the proteoforms of surface localized Rod A of Aspergillus flavus and determination of the mechanism of proteoform generation.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Proteomics, Aravind Medical Research Foundation, Aravind Eye Care System, Dr. G. Venkataswamy Eye Research Institute, No:1 Anna Nagar, Madurai, TN, India. Electronic address:

Fungal keratitis is a serious, potentially sight-threatening corneal infection that is more prevalent in the tropical parts of the world including India, and A. flavus and Fusarium solani are the predominant etiological agents. The surface of fungal conidia is covered by hydrophobin family proteins, effectively masking the conidial antigens from immune cells. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.016DOI Listing
December 2018

Dissecting the molecular effects of cigarette smoke on proteasome function.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:

Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More

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http://dx.doi.org/10.1016/j.jprot.2018.12.015DOI Listing
December 2018

Non-invasive MRI of brain clearance pathways using multiple echo time arterial spin labelling: An Aquaporin-4 study.

Neuroimage 2018 Dec 14. Epub 2018 Dec 14.

UCL Centre for Advanced Biomedical Imaging, Division of Medicine, UCL, London, UK.

There is currently a lack of non-invasive tools to assess water transport in healthy and pathological brain tissue. Aquaporin-4 (AQP4) water channels are central to many water transport mechanisms, and emerging evidence also suggests that AQP4 plays a key role in amyloid-β (Aβ) clearance, possibly via the glymphatic system. Here, we present the first non-invasive technique sensitive to AQP4 channels polarised at the blood-brain interface (BBI). Read More

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http://dx.doi.org/10.1016/j.neuroimage.2018.12.026DOI Listing
December 2018

A multiomic approach to characterize the temporal sequence in Alzheimer's disease-related pathology.

Neurobiol Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute of Biomedicine, University of Eastern Finland, Kuopio 70210, Finland. Electronic address:

No single-omic approach completely elucidates the multitude of alterations taking place in Alzheimer's disease (AD). Here, we coupled transcriptomic and phosphoproteomic approaches to determine the temporal sequence of changes in mRNA, protein, and phosphopeptide expression levels from human temporal cortical samples, with varying degree of AD-related pathology. This approach highlighted fluctuation in synaptic and mitochondrial function as the earliest pathological events in brain samples with AD-related pathology. Read More

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http://dx.doi.org/10.1016/j.nbd.2018.12.009DOI Listing
December 2018

Following spinal cord injury, PDE4B drives an acute, local inflammatory response and a chronic, systemic response exacerbated by gut dysbiosis and endotoxemia.

Neurobiol Dis 2018 Dec 14. Epub 2018 Dec 14.

Kentucky Spinal Cord Injury Research Center, University of Louisville, School of Medicine, 511 S. Floyd St., MDR 616, Louisville, KY 40202, USA; Department of Anatomical Science & Neurobiology, University of Louisville, School of Medicine, 511 S. Floyd St., MDR 616, Louisville, KY 40202, USA.

Emerging evidence links changes in the gut microbiome and intestinal barrier function to alterations in CNS function. We examined the role of endotoxin-responsive, cAMP-specific, Pde4 subfamily b (Pde4b) enzyme in gut dysbiosis induced neuro-inflammation and white matter loss following spinal cord injury (SCI). Using a thoracic contusion model in C57Bl/6 wild type female mice, SCI led to significant shifts in the gut bacterial community including an increase in the phylum Proteobacteria, which consists of endotoxin-harboring, gram-negative bacteria. Read More

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http://dx.doi.org/10.1016/j.nbd.2018.12.008DOI Listing
December 2018

Alginate/Chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles.

J Control Release 2018 Dec 14. Epub 2018 Dec 14.

School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 950 Atlantic Dr. NW, Atlanta, GA 30332, USA. Electronic address:

Enzymes with intracellular activity have significant potential to treat diseases. Protein nanoparticles (NPs) considerably enhance intracellular delivery of enzymes. We have previously shown that a Salmonella effector enzyme, AvrA, delivered by NPs is capable of modulating inflammatory signals in a murine dextran sulfate sodium (DSS) colitis model. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01683659183072
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http://dx.doi.org/10.1016/j.jconrel.2018.12.017DOI Listing
December 2018
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Enhanced intranasal insulin delivery by formulations and tumor protein-derived protein transduction domain as an absorption enhancer.

J Control Release 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmaceutics and Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

One of the key factors for successful development of an intranasal insulin formulation is an absorption enhancer that would deliver insulin efficiently across nasal membranes without causing damage to mucosa or inducing protein aggregation under physiological conditions. In the present study, a protein transduction domain (PTD1) and its L-form with the double substitution A6L and I8A (PTD4), derived from human translationally controlled tumor protein, were used as absorption enhancers. PTD4 exhibited higher compatibility with insulin in terms of biophysical properties analyzed using μDSC, DLS, and CD. Read More

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http://dx.doi.org/10.1016/j.jconrel.2018.12.023DOI Listing
December 2018

Biodegradable foam tray based on starches isolated from different Peruvian species.

Int J Biol Macromol 2018 Dec 14. Epub 2018 Dec 14.

Facultad de Ciencias Agropecuarias, Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo, Peru. Electronic address:

Starch was isolated from three Andean-native crops - arracacha (Arracacia xanthorrhiza), oca (Oxalis tuberosa), and sweet potato (Ipomoea batatas) - for use as a raw material for the production of foam trays. The starches were characterized in their proximal composition, crystallinity, microstructure and thermal properties. The sweet potato starch showed the highest amylose content (42. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2018.12.111DOI Listing
December 2018

Diurnal Regulation of Sphingolipids in Blood.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 14. Epub 2018 Dec 14.

Department of General Pharmacology and Toxicology, Goethe University Hospital, Frankfurt am Main, Germany.

Key homeostatic functions are regulated in a diurnal manner and a miss-alignment of such rhythms is believed to contribute to the pathophysiology of several diseases. Signaling sphingolipids (SLs) in plasma such as sphingosine 1-phosphate control lymphocytic trafficking, vascular reactivity and platelet activity, physiological functions all of which display a diurnal rhythm themselves. However, the rhythmicity of SL metabolism in plasma and its potential causes have not been sufficiently investigated so far. Read More

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http://dx.doi.org/10.1016/j.bbalip.2018.12.001DOI Listing
December 2018

Single-molecule 3D imaging of human plasma intermediate-density lipoproteins reveals a polyhedral structure.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 14. Epub 2018 Dec 14.

The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

Intermediate-density lipoproteins (IDLs), the remnants of very-low-density lipoproteins via lipolysis, are rich in cholesteryl ester and are correlated with cardiovascular disease. Despite pharmacological interests in IDL, the three-dimensional (3D) structure of IDLs is still undetermined due to their variation in size, compositions, and dynamic structure. To explore the 3D structure of IDL, we reconstructed 3D density maps from each individual IDL particles using cryo-electron microscopy (cryo-EM) and individual-particle electron tomography (IPET, without averaging from different molecules). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13881981183037
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http://dx.doi.org/10.1016/j.bbalip.2018.12.004DOI Listing
December 2018
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IgE blockade in autoimmunity: Omalizumab induced remission of bullous pemphigoid.

Clin Immunol 2018 Dec 14. Epub 2018 Dec 14.

Department of Clinical Immunology, Royal Perth Hospital, Perth, WA 6000, Australia; Institute of Immunology and Infectious Diseases (IIID), Murdoch University, Murdoch, WA 6150, Australia. Electronic address:

Bullous pemphigoid (BP) is a blistering dermopathy and a prototypic antibody-mediated autoimmune disease. Detection of IgG autoantibodies against hemidesmosomal proteins BP180 and/or BP230 are diagnostic and levels can correlate with disease activity. Therapies include corticosteroids and oral immunosuppressants, while intravenous immunoglobulin and rituximab are reserved for treatment resistant cases. Read More

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http://dx.doi.org/10.1016/j.clim.2018.12.015DOI Listing
December 2018
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Heterologous expression and characterization of Penicillium citrinum nuclease P1 in Aspergillus niger and its application in the preparation of nucleotides.

Protein Expr Purif 2018 Dec 14. Epub 2018 Dec 14.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, 510006, China; Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou, 510006, China. Electronic address:

Nuclease P1 has been used for the industrial production of 5'-monouncleotides from yeast RNA due to it's single-strand specific nucleases activity. The high demand for 5'-nucleotides in the manufacture of food and drugs requires the commercial scale production of nuclease P1, but the fermentation level of nuclease P1 from Penicillium citrinum still remains low. In this study, the Penicillium citrinum nuclease P1 gene (nuc P1) was heterologously expressed in A. Read More

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http://dx.doi.org/10.1016/j.pep.2018.12.004DOI Listing
December 2018

Regulation of asymmetries in the kinetics and protein numbers of bacterial gene expression.

Biochim Biophys Acta Gene Regul Mech 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Biosystem Dynamics, BioMediTech Institute and Faculty of Biomedical Sciences and Engineering, Tampere University of Technology, 33101 Tampere, Finland. Electronic address:

Genetic circuits change the status quo of cellular processes when their protein numbers cross thresholds. We investigate the regulation of RNA and protein threshold crossing propensities in Escherichia coli. From in vivo single RNA time-lapse microscopy data from multiple promoters, mutants, induction schemes and media, we study the asymmetry and tailedness (quantified by the skewness and kurtosis, respectively) of the distributions of time intervals between transcription events. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2018.12.005DOI Listing
December 2018

DHA inhibits proliferation and induces ferroptosis of leukemia cells through autophagy dependent degradation of ferritin.

Free Radic Biol Med 2018 Dec 14. Epub 2018 Dec 14.

Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China; Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China. Electronic address:

Dihydroartemisinin (DHA) has been shown to be capable of inhibiting cancer growth, whereas it remains largely elusive that the underlying molecular mechanism of DHA induced acute myeloid leukemia (AML) cell death. In the present study, we examined the effects of DHA on the proliferation and ferroptosis of AML cells as well as to elucidate the underlying molecular mechanisms. We found that DHA strongly inhibited the viability of AML cell lines and arrest cell cycle at G0/G1 phase. Read More

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http://dx.doi.org/10.1016/j.freeradbiomed.2018.12.011DOI Listing
December 2018

Aquatic animal viruses mediated immune evasion in their host.

Authors:
Fei Ke Qi-Ya Zhang

Fish Shellfish Immunol 2018 Dec 14. Epub 2018 Dec 14.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address:

Viruses are important and lethal pathogens that hamper aquatic animals. The result of the battle between host and virus would determine the occurrence of diseases. The host will fight against virus infection with various responses such as innate immunity, adaptive immunity, apoptosis, and so on. Read More

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http://dx.doi.org/10.1016/j.fsi.2018.12.027DOI Listing
December 2018

TCF7L2 and EGR1 synergistic activation of transcription of LCN2 via an ERK1/2-dependent pathway in esophageal squamous cell carcinoma cells.

Cell Signal 2018 Dec 14. Epub 2018 Dec 14.

Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China; Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Genes Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. Electronic address:

High level expression of lipocalin 2 (LCN2) usually indicates poor prognosis in esophageal squamous cell carcinoma (ESCC) and many other cancers. Our previous study showed LCN2 promotes migration and invasion of ESCC cells through a novel positive feedback loop. However, the key transcription activation protein (KTAP) in the loop had not yet been identified. Read More

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http://dx.doi.org/10.1016/j.cellsig.2018.12.007DOI Listing
December 2018

Goose MAVS functions in RIG-I-mediated IFN-β signaling activation.

Dev Comp Immunol 2018 Dec 14. Epub 2018 Dec 14.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China. Electronic address:

Mitochondrial antiviral-signaling protein (MAVS) is an essential adaptor protein in retinoic acid-inducible gene I (RIG-I)-mediated antiviral innate immunity in mammals. In this study, the goose MAVS gene (goMAVS) was identified. The 2,019 bp-long goMAVS exhibits 96. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0145305X183047
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http://dx.doi.org/10.1016/j.dci.2018.12.006DOI Listing
December 2018
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Development of reverse genetics system for small ruminant morbillivirus: rescuing recombinant virus to express Echinococcus granulosus EG95 antigen.

Virus Res 2018 Dec 14. Epub 2018 Dec 14.

OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China. Electronic address:

Peste des petits ruminants and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are small ruminant morbillivirus (SRMV) and Echinococcus granulosus (E. granulosus), respectively. Read More

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http://dx.doi.org/10.1016/j.virusres.2018.12.008DOI Listing
December 2018

Metal chelators for the inhibition of the lymphocytic choriomeningitis virus endonuclease domain.

Antiviral Res 2018 Dec 14. Epub 2018 Dec 14.

Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 163 avenue de Luminy, 13288, Marseille, France. Electronic address:

Arenaviridae is a viral family whose members are associated with rodent-transmitted infections to humans responsible of severe diseases. The current lack of a vaccine and limited therapeutic options make the development of efficacious drugs of high priority. The cap-snatching mechanism of transcription of Arenavirus performed by the endonuclease domain of the L-protein is unique and essential, so we developed a drug design program targeting the endonuclease activity of the prototypic Lymphocytic ChorioMeningitis Virus. Read More

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http://dx.doi.org/10.1016/j.antiviral.2018.12.008DOI Listing
December 2018

Bioluminescence imaging of Arc expression in mouse brain under acute and chronic exposure to pesticides.

Neurotoxicology 2018 Dec 14. Epub 2018 Dec 14.

Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, Japan. Electronic address:

Exposure to pesticides can induce neurobehavioral effects in rodents, as well as in other mammals, including humans. However, the effects of the toxicity of pesticides on the central nervous system (CNS) remain largely unclear. The expression of the activity-regulated cytoskeleton-associated protein gene (Arc) is induced in a neuronal-activity-dependent manner and is implicated in synaptic and experience-dependent plasticity. Read More

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http://dx.doi.org/10.1016/j.neuro.2018.12.003DOI Listing
December 2018

R125H, W240S, C386R, and V507I SLC4A11 mutations associated with corneal endothelial dystrophy affect the transporter function but not trafficking in PS120 cells.

Exp Eye Res 2018 Dec 14. Epub 2018 Dec 14.

School of Optometry, Indiana University Bloomington, Bloomington, IN, USA. Electronic address:

SLC4A11 mutations are associated with Fuchs' endothelial corneal dystrophy (FECD), congenital hereditary endothelial dystrophy (CHED) and Harboyan syndrome (endothelial dystrophy with auditory deficiency). Mice with genetically ablated Slc4a11 recapitulate CHED, exhibiting significant corneal edema and altered endothelial morphology. We recently demonstrated that SLC4A11 functions as an NH sensitive, electrogenic H transporter. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00144835183051
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http://dx.doi.org/10.1016/j.exer.2018.12.003DOI Listing
December 2018
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KU-596 decreases mitochondrial superoxide and improves bioenergetics following downregulation of manganese superoxide dismutase in diabetic sensory neurons.

Exp Neurol 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmacology and Toxicology, University of Kansas, 5064 Malott Hall, 1251 Wescoe Hall Dr., Lawrence, KS 66045, USA. Electronic address:

Neuronal mitochondrial dysfunction and oxidative stress are key pathophysiologic mechanisms of diabetic peripheral neuropathy (DPN). KU-596 is a small molecule modulator of heat shock protein 90 (Hsp90) that can reverse clinically relevant measures of DPN in diabetic animal models. Mechanistically, drug efficacy requires Hsp70 and correlates with improving mitochondrial maximal respiratory capacity (MRC) and decreasing oxidative stress in diabetic sensory neurons. Read More

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http://dx.doi.org/10.1016/j.expneurol.2018.12.006DOI Listing
December 2018

PPARα suppresses Th17 cell differentiation through IL-6/STAT3 /RORγt pathway in experimental autoimmune myocarditis.

Exp Cell Res 2018 Dec 14. Epub 2018 Dec 14.

Medical College of Xiamen University, Xiamen 361102, China. Electronic address:

Family members of peroxisome proliferator-activated receptors (PPARs), such as PPARγ, have been shown to be effective in regulating T helper 17 (Th17) cell differentiation. However, whether PPARα, another important family member of PPARs, contributes to Th17 cell differentiation remains controversial. In the present study, we show that PPARα may be a negative regulator of Th17 cell differentiation. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.005DOI Listing
December 2018

Pluripotency markers in tissue and cultivated cells in vitro of different regions of human amniotic epithelium.

Exp Cell Res 2018 Dec 14. Epub 2018 Dec 14.

Instituto Nacional de Perinatología, Departamento de Fisiología y Desarrollo Celular, Ciudad de México, México. Electronic address:

Studies have described the presence of pluripotent markers in vivo and in vitro in human amnion. However, the amnion can be divided into reflected, placental and umbilical regions that are anatomically and functionally heterogeneous. Here, we evaluated the expression of pluripotency markers in tissue and cultivated cells in vitro of different regions of human amnion. Read More

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http://dx.doi.org/10.1016/j.yexcr.2018.12.007DOI Listing
December 2018

Macromolecular crowding and membrane binding proteins: the case of phospholipase A.

Chem Phys Lipids 2018 Dec 14. Epub 2018 Dec 14.

Division of Chemistry, School of Pharmacy and Biological Sciences, University of Brighton, BN2 4GL, United Kingdom. Electronic address:

Cells contain high levels of macromolecular crowding; understanding how macromolecular crowding impacts the behaviour of biological systems can give new insights into biological phenomena and disease pathologies. In this study, we assess the effect of macromolecular crowding on the catalytic activity of the biomembrane binding protein phospholipase A (PLA). Using 3D-printed equilibrium dialysis chambers we show that macromolecular crowding increases the binding of PLA to lipid vesicles. Read More

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http://dx.doi.org/10.1016/j.chemphyslip.2018.12.006DOI Listing
December 2018

Association between Serum Resistin, Adiposity Measures and Inflammatory Makers in Women Without Cardiovascular Diseases.

Chem Phys Lipids 2018 Dec 14. Epub 2018 Dec 14.

Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

Aims: The convergence of obesity and inflammation in the pathogenesis of cardiovascular diseases (CVD) has been recognized over the past decade. Resistin has emerged as a novel secreted protein with links to both insulin resistance and inflammation. We aimed to investigate the relationship between changes in serum resistin levels with metabolic parameters, including obesity and inflammatory markers in women free of CVD. Read More

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http://dx.doi.org/10.1016/j.chemphyslip.2018.12.005DOI Listing
December 2018

Fluvastatin-mediated down-regulation of SATB1 affects aggressive phenotypes of human non-small-cell lung cancer cell line H292.

Life Sci 2018 Dec 14. Epub 2018 Dec 14.

Department of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Aims: Fluvastatin reduces tumor proliferation and increased apoptotic activity in various cancers. Special AT-rich sequence binding protein 1 (SATB1) is a genome organizer that reprogrammes the gene transcription profiles of tumors to promote growth and metastasis. The antitumor effect and molecular mechanisms of fluvastatin on lung cancer is poorly understood. Read More

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http://dx.doi.org/10.1016/j.lfs.2018.12.022DOI Listing
December 2018

Rooting of eucalypt cuttings as a problem-solving oriented model in plant biology.

Prog Biophys Mol Biol 2018 Dec 14. Epub 2018 Dec 14.

Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), P.O. Box 15005, Porto Alegre, RS, 91501-970, Brazil. Electronic address:

Species of Eucalyptus are some of the most planted trees in the world, providing fiber, cellulose, energy, and wood for construction and furniture in renewable fashion, with the added advantage of fixing large amounts of atmospheric carbon. The efficiency of eucalypts in forestry relies mostly on the clonal propagation of selected genotypes both as pure species and interspecific hybrids. The formation of new roots from cambium tissues at the base of cuttings, referred to as adventitious rooting (AR), is essential for accomplishing clonal propagation successfully. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00796107183022
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http://dx.doi.org/10.1016/j.pbiomolbio.2018.12.007DOI Listing
December 2018
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Spatial presentation of cholesterol units on a DNA cube as a determinant of membrane protein-mimicking functions.

J Am Chem Soc 2018 Dec 17. Epub 2018 Dec 17.

Cells use membrane proteins as gatekeepers to transport ions and molecules, catalyze reactions, relay signals, and interact with other cells. DNA nanostructures with lipidic anchors are promising as membrane protein mimics because of their high tuneability. However, the design features specifying DNA nanostructure's functions in lipid membranes are yet to be fully understood. Read More

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http://dx.doi.org/10.1021/jacs.8b11898DOI Listing
December 2018

The problem of genetic code misreading during protein synthesis.

Yeast 2018 Dec 17. Epub 2018 Dec 17.

Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, 21228, United States of America.

Saccharomyces cerevisiae has been an important model for determining the frequency of translational misreading events, those in which a tRNA pairs incorrectly to the mRNA and inserts an amino acid not specified by the codon in the mRNA. Misreading errors have been quantified in vivo using reporter protein systems or mass spectrometry with both approaches converging on a simple model for most misreading. The available data show that misreading tRNAs must form stereotypical base mismatches that correspond to those that can mimic Watson-Crick base pairs when formed in the ribosomal A site. Read More

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http://dx.doi.org/10.1002/yea.3374DOI Listing
December 2018
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