6,818,143 results match your criteria Protein & Peptide Letters [Journal]

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Multisite measurement of regional oxygen saturation in Fontan patients with or without protein-losing enteropathy at rest and during exercise.

Pediatr Res 2019 Feb 22. Epub 2019 Feb 22.

Department of Pediatric Cardiology, University of Erlangen-Nuernberg, Loschgestraße 15, 91054, Erlangen, Germany.

Background: Protein-losing enteropathy (PLE) is a severe complication of Fontan circulation with increased risk of end-organ dysfunction. We evaluated tissue oxygenation via near-infrared spectroscopy (NIRS) at different exercise levels in Fontan patients.

Materials And Methods: Assessment of multisite NIRS during cycle ergometer exercise and daily activities in three groups: Fontan patients with PLE; without PLE; patients with dextro-transposition of the great arteries (d-TGA); comparing univentricular with biventricular circulation and Fontan with/without PLE. Read More

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http://dx.doi.org/10.1038/s41390-019-0346-3DOI Listing
February 2019

Density of small dendritic spines and microtubule-associated-protein-2 immunoreactivity in the primary auditory cortex of subjects with schizophrenia.

Neuropsychopharmacology 2019 Feb 22. Epub 2019 Feb 22.

Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA.

Previously, we demonstrated that dendritic spine density (DSD) in deep layer 3 of the primary auditory cortex (A1) lower, due to having fewer small spines, in subjects with schizophrenia (SZ) than non-psychiatric control (NPC) subjects. We also previously demonstrated that microtubule-associated-protein-2 immunoreactivity (MAP2-IR) in A1 deep layer 3 is lower, and positively correlated with DSD, in SZ subjects. Here, we first sought to confirm these findings in an independent cohort of 25 SZ-NPC subject pairs (cohort 1). Read More

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http://dx.doi.org/10.1038/s41386-019-0350-7DOI Listing
February 2019

Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts.

Acta Biomater 2019 Feb 19. Epub 2019 Feb 19.

WPI Research Initiative for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan; Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. Electronic address:

The proliferation epidermal growth factor (EGF) is known to acquire contradictory apoptotic activities upon conjugation with gold nanoparticles (GNPs) through hitherto unknown mechanisms. Here, we identified an essential role of membrane rafts in the drastic activity switching of EGF-GNPs through the following intracellular signaling. 1) In contrast to the rapid diffusion of activated EGF receptor after the soluble EGF stimulation, the receptor is confined within membrane rafts upon binding to the EGF-GNPs. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.026DOI Listing
February 2019

Anaphylaxis to hidden pea protein: a Canadian pediatric case series.

J Allergy Clin Immunol Pract 2019 Feb 19. Epub 2019 Feb 19.

Division of Pediatric Allergy and Clinical Immunology, Department of Pediatrics, Montreal Children's Hospital, Montreal, Quebec, Canada.

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http://dx.doi.org/10.1016/j.jaip.2019.02.010DOI Listing
February 2019

Vitamin D-binding protein-loaded PLGA nanoparticles suppress Alzheimer's disease-related pathology in 5XFAD mice.

Nanomedicine 2019 Feb 19. Epub 2019 Feb 19.

Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Republic of Korea. Electronic address:

The aggregation and accumulation of amyloid beta (Aβ) peptide is believed to be the primary cause of Alzheimer's disease (AD) pathogenesis. Vitamin D-binding protein (DBP) can attenuate Aβ aggregation and accumulation. A biocompatible polymer poly (-lactic acid-co-glycolic acid) (PLGA) can be loaded with therapeutic agents and control the rate of their release. Read More

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http://dx.doi.org/10.1016/j.nano.2019.02.004DOI Listing
February 2019

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Nanomedicine 2019 Feb 19. Epub 2019 Feb 19.

Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD. Electronic address:

The complement system plays an essential role in both innate and adaptive immunity. The traditional understanding of this system comes from studies investigating complement proteins produced by the liver and present in plasma to "complement" the immune cell-mediated response to invading pathogens. Recently, it has been reported that immune cells including, but not limited to, T-cells and monocytes, express complement proteins. Read More

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http://dx.doi.org/10.1016/j.nano.2019.02.002DOI Listing
February 2019

Gene cloning and expression of a partial sequence of Hirudomacin, an antimicrobial protein that is increased in leech (Hirudo nipponica Whitman) after a blood meal.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 19. Epub 2019 Feb 19.

Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.

The novel antimicrobial gene Hirudomacin (Hmc), with a 249-bp cDNA, encodes a mature protein of 61 amino acids and a 22-amino acid signal peptide. Hmc exhibits the highest similarity, at 90.1%, with macin family members found in the salivary gland of the leech Hirudo nipponica Whitman. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.02.005DOI Listing
February 2019

Variation of Fetuin-A in maternal and fetal serum during human parturition.

J Gynecol Obstet Hum Reprod 2019 Feb 19. Epub 2019 Feb 19.

Department of Obstetrics and Gynecology, Beaujon, AP-HP Hospital, Clichy and Paris Diderot University, France; Inserm UMR1139 « Pharmatoxicology and Placenta », Paris Descartes University, Faculty of Pharmacys and Hospitalo-university Department « Risk and Pregnancy », France; PremUp Foundation, Paris, France. Electronic address:

Background: parturition involves multiple signalling pathways and most advances in research underline the importance of fetal development and maturation in the timing of labour, especially the fetal pituitary-adrenal axis. But, there is currently no consensual hypothesis on all the physiological processes responsible for human parturition.

Methods: sixty low-risk pregnant women were enrolled in a prospective cohort. Read More

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http://dx.doi.org/10.1016/j.jogoh.2019.02.003DOI Listing
February 2019

Exploring the influence of the surface proteins on probiotic effects performed by Lactobacillus pentosus HC-2 using transcriptome analysis in Litopenaeus vannamei midgut.

Fish Shellfish Immunol 2019 Feb 19. Epub 2019 Feb 19.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; CAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266400, China. Electronic address:

In order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCl) treatment. The results showed that the shrimp fed with normal HC-2 produced much higher immune and digestion related enzymes than the control group or LiCl-treated HC-2 group to defense the Vibrio parahaemolyticus E1 infection. We obtained total over 275,099 unigenes from L. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.027DOI Listing
February 2019

Targeting ERK1/2 protein-serine/threonine kinases in human cancers.

Authors:
Robert Roskoski

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC, 28742-8814, United States. Electronic address:

ERK1 and ERK2 are key protein kinases that contribute to the Ras-Raf-MEK-ERK MAP kinase signalling module. This pathway participates in the control of numerous processes including apoptosis, cell proliferation, the immune response, nervous system function, and RNA synthesis and processing. MEK1/2 activate human ERK1/2 by first catalyzing the phosphorylation of Y204/187 and then T202/185, both residues of which occur within the activation segment. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.01.039DOI Listing
February 2019

Dihydrotanshinone exhibits an anti-inflammatory effect in vitro and in vivo through blocking TLR4 dimerization.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address:

Dihydrotanshinone (DHT), one of the major ingredients of Salvia miltiorrhiza Bunge (Danshen), displays many bioactivities. However, the activity and underlying mechanism of DHT in anti-inflammation have not yet been elucidated. In this study, we investigated the anti-inflammatory activity and molecular mechanism of action of DHT both in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.017DOI Listing
February 2019

The effect of probiotic and synbiotic supplementation on biomarkers of inflammation and oxidative stress in diabetic patients: a systematic review and meta-analysis of randomized controlled trials.

Pharmacol Res 2019 Feb 19. Epub 2019 Feb 19.

Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Shipping warehouse No. 5, Beijing, 100700, China. Electronic address:

The role of gut microbiota in the management of diabetes has been shown. Several current trials are investigating the effect of probiotics and prebiotics, which are widely used to modulate intestinal microbiota, on inflammatory factors and biomarkers of oxidative stress in diabetic patients; however, their findings are controversial. The aim of the current meta-analysis was to evaluate the effects of probiotic and synbiotic supplementation on levels of serum high-sensitivity C-reactive protein (hs-CRP) and biomarkers of oxidative stress in diabetic patients. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.02.016DOI Listing
February 2019

Bis-iridoid and iridoid glycosides: Viral protein R inhibitors from Picrorhiza kurroa collected in Myanmar.

Fitoterapia 2019 Feb 19. Epub 2019 Feb 19.

Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan. Electronic address:

Four new bis-iridoid glycosides, saungmaygaosides A-D (1-4), and six known iridoid glycosides (5-10) were isolated from the n-butanol extract of the stems of Picrorhiza kurroa collected in Myanmar. Their structures were elucidated by extensive spectroscopic techniques. All of the isolates were assayed for anti-Vpr activity, using TREx-HeLa-Vpr cells. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.02.016DOI Listing
February 2019

Cloning of nitrite reductase gene from Haematococcus pluvialis and transcription and enzymatic activity analysis at different nitrate and phosphorus concentration.

Gene 2019 Feb 19. Epub 2019 Feb 19.

College of Marine Life Sciences, Ocean University of China/Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Qingdao 266003, Shandong, China.

Haematococcus pluvialis is an economic microalga to produce astaxathin. To study the nitrogen metabolic process of H. pluvialis, the transcription level and enzyme content of nitrite reductase at different nitrate and phosphorus concentrations were studied. Read More

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http://dx.doi.org/10.1016/j.gene.2019.01.042DOI Listing
February 2019

Long non-coding RNA PVT1 promotes autophagy as ceRNA to target ATG3 by sponging microRNA-365 in hepatocellular carcinoma.

Gene 2019 Feb 19. Epub 2019 Feb 19.

Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China. Electronic address:

The pathogenesis and the underlying mechanisms of hepatocellular carcinoma (HCC) remain unclear. LncRNA plasmacytoma variant translocation 1 (PVT1) is an oncogene in a variety of cancers. The role of PVT1 in HCC progression is not completely understood. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.036DOI Listing
February 2019

Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart.

Gene 2019 Feb 19. Epub 2019 Feb 19.

Biomedical Research Institute, College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, Hubei Province 430081, PR China. Electronic address:

WDR1 is a major cofactor of the actin depolymerizing factor (ADF)/cofilin, accelerating ADF/cofilin-mediated actin disassembly. We had previously showed that WDR1-mediated actin dynamics is required for postnatal myocardial growth and adult myocardial maintenance in mice, in which the detailed phenotypes of adult cardiomyocyte-specific Wdr1 deletion mice had not been analyzed. In this study, we systematically analyzed the role of Wdr1 in adult mouse heart. Read More

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http://dx.doi.org/10.1016/j.gene.2019.02.023DOI Listing
February 2019

Imaging-based assays for investigating functions of the RNA polymerase II elongation factor Elongin and the Elongin ubiquitin ligase.

Methods 2019 Feb 19. Epub 2019 Feb 19.

Stowers Institute for Medical Research, 1000 E 50(th) Street, Kansas City, MO 64110, United States; Department of Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, United States.

Elongin A binds to Elongins B and C to form the RNA polymerase II transcription elongation factor Elongin. It also functions as the substrate recognition subunit of a ubiquitin ligase that is formed by binding of Elongin to Cullin protein CUL5 and RING finger protein RBX2 and that targets RNA polymerase II for ubiquitination. In this article, we describe use of acceptor photobleaching fluorescence resonance energy transfer (AP-FRET) and laser microirradiation-based assays to study regulated assembly of the Elongin ubiquitin ligase and its recruitment to regions of localized DNA damage. Read More

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http://dx.doi.org/10.1016/j.ymeth.2019.02.015DOI Listing
February 2019

Free energy calculations of RNA interactions.

Methods 2019 Feb 19. Epub 2019 Feb 19.

Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, SE-751 24, Uppsala, Sweden. Electronic address:

This review discusses the use of molecular dynamics free energy calculations for characterizing RNA interactions, with particular emphasis on molecular recognition events involved in mRNA translation on the ribosome. The general methodology for efficient free energy calculations is outlined and our specific implementation for binding free energy changes due to base mutations in mRNA and tRNA is described. We show that there are a number of key problems related to the accuracy of protein synthesis that can be addressed with this type of computational approach and several such examples are discussed in detail. Read More

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http://dx.doi.org/10.1016/j.ymeth.2019.02.014DOI Listing
February 2019

Dynamic oligomerization of hRAGE's transmembrane and cytoplasmic domains within SDS micelles.

Int J Biol Macromol 2019 Feb 19. Epub 2019 Feb 19.

Department of Biological Sciences (Biochemistry Program), Faculty of Science, Kuwait University, P.O. Box 5969, 13060, Kuwait.

The human Receptor for Advanced Glycation End Products (hRAGE) is a pattern recognition receptor implicated in inflammation and adhesion. It is involved in both innate and adaptive immunity. Its aberrant signaling is tied to the pathogenesis of diabetic complications, neurodegenerative disorders, and chronic inflammatory responses. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.02.108DOI Listing
February 2019

An In Vitro elucidation of the antiaggregatory potential of Diosmin over thermally induced unfolding of hen egg white lysozyme; A preventive quest for lysozyme amyloidosis.

Int J Biol Macromol 2019 Feb 19. Epub 2019 Feb 19.

Protein Amyloid Research Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, UP 202002, India. Electronic address:

Protein misfolding diseases are associated with human pathologies. These neurodegenerative diseases remain challenging task for researchers because of their adverse effect on vital organs system. Lysozyme amyloidosis is also associated with multi-organ dysfunction. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.02.107DOI Listing
February 2019

Characterization of HIV-1 Genotype Specific Antigens for the Detection of Recent and Long-term HIV-1 Infection in China.

Virus Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, China; Guangdong Provincial Key Laboratory of Tropical Disease Research, Guangzhou, China; Dermatology Hospital, Southern Medical University, Guangzhou, China. Electronic address:

To characterize HIV-1 gp41 as an antigen for developing HIV-1 incidence assay and to investigate the impact of HIV-1 genetic diversity on the assay performance, a number of truncated peptides were synthesized to identify the immunodominant epitopes (IDEs) of HIV-1 gp41 protein. Subsequently, the mixed peptides (MP3) or the recombinant protein (MP4) containing HIV-1 gp41 IDEs of the major HIV-1 genotype CRF01_AE, CRF07_BC/ CRF08_BC and subtype B in China were used to verify the sensitivity and specificity of HIV-1 recency testing. We identified the QKFLG and GKIIC motifs located in the loop region of HIV-1 gp41 as the two major IDEs. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.02.010DOI Listing
February 2019

Molecular analysis of the full-length F gene of Brazilian strains of canine distemper virus shows lineage co-circulation and variability between field and vaccine strains.

Virus Res 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid - Campus Universitário, CEP 86057-970 - Londrina, PO Box 10011, Paraná, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina. Rodovia Celso Garcia Cid - Campus Universitário, CEP 86057-970 - Londrina, PO Box 10011, Paraná, Brazil.

Canine distemper is a highly contagious systemic viral disease, with worldwide distribution that affects a wide variety of terrestrial carnivores. This study characterized full-length fusion (F) genes from 15 Brazilian wild-type canine distemper virus (CDV) strains collected between 2003-2004 (n = 6) and 2013-2016 (n = 9). Using deduced amino acid (aa) sequence analysis, 14 strains were classified into Europe 1/South America 1 (EU1/SA1) lineage, with a temporal clustering into past (2003-2004) and contemporary (2013-2016) strains. Read More

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http://dx.doi.org/10.1016/j.virusres.2019.02.009DOI Listing
February 2019

FAM134B promotes esophageal squamous cell carcinoma in vitro and its correlations with clinicopathologic features.

Hum Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Australia. Electronic address:

Family with sequence similarity 134, member B (FAM134B) is an autophagy regulator of endoplasmic reticulum first discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC). The present study examined the functional behavior of FAM134B in cancer cells and the association of FAM134B expression with clinicopathologic factors in patients with ESCC. Expression at both the mRNA and protein levels was investigated using real-time polymerase chain reaction and immunohistochemistry. Read More

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http://dx.doi.org/10.1016/j.humpath.2018.11.033DOI Listing
February 2019

Reactive cholangiocytes differentiate into proliferative hepatocytes with efficient DNA repair in mice with chronic liver injury.

J Hepatol 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Hepato-gastroenterology, Institut de Recherche Expérimentale et Clinique, Université catholique de Louvain, Brussels, Belgium. Electronic address:

Background And Aim: Chronic liver diseases are characterized by expansion of the small immature cholangiocytes - a mechanism named ductular reaction (DR) - which have the capacity to differentiate in hepatocytes. We investigated the kinetics of DR differentiation into hepatocytes as well as several important features as functional maturity, clonal expansion and resistant to stress of the newly formed hepatocytes in mice with long-term liver damage.

Methods: We tracked cholangiocytes using osteopontin-iCreER and hepatocytes with AAV8-TBG-Cre. Read More

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http://dx.doi.org/10.1016/j.jhep.2019.02.003DOI Listing
February 2019

Clinical and genetic characteristics of unusual G12P [11] rotavirus strains recovered from neonates: A study from Pune, Western India.

Infect Genet Evol 2019 Feb 19. Epub 2019 Feb 19.

Enteric Viruses Group, ICMR-National Institute of Virology, Pune, India. Electronic address:

Rotavirus infections in neonates are generally nosocomial, and differ from pediatric infections both clinically and epidemiologically. These infections are predominantly asymptomatic and often associated with unusual strains. Globally, so far limited data is available on rotavirus infections in neonates admitted at Neonatal Intensive Care Unit. Read More

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http://dx.doi.org/10.1016/j.meegid.2019.02.014DOI Listing
February 2019

Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling.

Biochim Biophys Acta Mol Basis Dis 2019 Feb 19. Epub 2019 Feb 19.

Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan 430060, China; Department of Cardiology, The Fifth Affiliated Hospital of Xin Jiang Medical University, Urumchi 830001, China; Qianjiang Central Hospital of Hubei Province, Qianjiang 433100, China. Electronic address:

Pathological cardiac hypertrophy, which may lead to heart failure and sudden death, can be affected by multiple factors. In our previous study, we revealed that IKKi deficiency induced cardiac hypertrophy through the activation of the AKT and NF-kB signaling pathway in response to aortic banding (AB). Non-coding RNAs, mainly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), play a crucial role in normal developmental and pathological processes. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.02.014DOI Listing
February 2019

Using Focus Ion Beam to Prepare Crystal Lamella for Electron Diffraction.

J Struct Biol 2019 Feb 19. Epub 2019 Feb 19.

Key Laboratory of Protein Sciences (Tsinghua University), Ministry of Education, Beijing, China; School of Life Sciences, Tsinghua University, Beijing, China. Electronic address:

Electron diffraction provides a powerful tool to solve the structures of small protein crystals. However, strong interactions between the electrons and the materials limit the application of the electron crystallographic method on large protein crystals with micrometer or larger sizes. Here, we used the focused ion beam (FIB) equipped on the scanning electron microscope (SEM) to mill a large crystal to thin lamella. Read More

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http://dx.doi.org/10.1016/j.jsb.2019.02.004DOI Listing
February 2019

Cyst Fluid Biosignature to Predict Intraductal Papillary Mucinous Neoplasms of the Pancreas with High Malignant Potential.

J Am Coll Surg 2019 Feb 19. Epub 2019 Feb 19.

DNA services; University of Illinois at Chicago, Chicago, IL.

Background: Current standard-of-care technologies such as imaging and cyst fluid analysis are unable to consistently distinguish intraductal papillary mucinous neoplasms of the pancreas (IPMN) at high-risk of pancreatic cancer from low-risk IPMN. The objective was to create a single-platform assay to identify IPMN that are at high-risk for malignant progression.

Study Design: Building on the Verona International Consensus Conference BD-IPMN biomarker study, specific protein, cytokine, mucin, DNA, and miRNA cyst fluid targets were identified for creation of a q-PCR based assay as we have previously published. Read More

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http://dx.doi.org/10.1016/j.jamcollsurg.2019.02.040DOI Listing
February 2019

Dihydrotestosterone activates AP-1 in LNCaP prostate cancer cells.

Int J Biochem Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Department of Medical Biochemistry and Molecular Biology, D-66421 Homburg, Germany.

A cross-talk between androgen/androgen receptor signaling and the AP-1 transcription factor has been proposed. In this study, we asked whether activation of AP-1 modifies androgen-responsive gene transcription, and whether androgens effect AP-1-regulated gene transcription. We show that activation of AP-1 via expression of a constitutively active mutant of mitogen-activated/extracellular signal responsive kinase kinase (MEK) kinase-1 did not increase the activity of the androgen-responsive probasin promoter. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.02.005DOI Listing
February 2019

Protein and surface expression of HCN2 and HCN4 subunits in mesocorticolimbic areas after cocaine sensitization.

Neurochem Int 2019 Feb 19. Epub 2019 Feb 19.

Physiology Department, University of Puerto Rico, School of Medicine, Medical Sciences Campus, San Juan, 00936-5067, Puerto Rico. Electronic address:

The I is a mixed depolarizing current present in neurons which, upon activation by hyperpolarization, modulates neuronal excitability in the mesocorticolimbic (MCL) system, an area which regulates emotions such as pleasure, reward, and motivation. Its biophysical properties are determined by HCN protein expression profiles, specifically HCN subunits 1-4. Previously, we reported that cocaine-induced behavioral sensitization increases HCN2 protein expression in all MCL areas with the Ventral Tegmental Area (VTA) showing the most significant increase. Read More

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http://dx.doi.org/10.1016/j.neuint.2019.02.004DOI Listing
February 2019

Divergent Response to Cannabinoid Receptor Stimulation in High and Low Stress-Induced Analgesia Mouse Lines Is Associated with Differential G-Protein Activation.

Neuroscience 2019 Feb 19. Epub 2019 Feb 19.

Faculty of Pharmacy with the Laboratory Medicine Division, Department of Pharmacodynamics, Medical University of Warsaw, Centre for Preclinical Research and Technology, Warsaw, Poland; Laboratory of Neurogenomics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland; Department of Internal Medicine, Hypertension and Vascular Diseases, Medical University of Warsaw, Warsaw, Poland.

Bidirectional selection of mice for high (HA) and low (LA) swim stress-induced analgesia (SSIA) is associated with a divergent response to opioids. In the current study, we investigated whether the genetic divergence in opioid system activity between HA and LA mice also affects cannabinoid sensitivity. Additionally, we also investigated whether the endocannabinoid system mediates SSIA in these lines. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.02.015DOI Listing
February 2019

Transcriptomic insight into antimicrobial peptide factors involved in the prophylactic immunity of crowded Mythimna separata larvae.

Dev Comp Immunol 2019 Feb 19. Epub 2019 Feb 19.

College of Horticulture and Plant Protection, Yangzhou University, Wenhui East Road, NO. 48, Yangzhou, Jiangsu Province, 225009, China.

Similar to pathogenic infection, a high population density alters insect prophylactic immunity. Antimicrobial peptides (AMPs) are known to play critical roles in an insect's humoral immune response to microbial infection. We applied RNA sequencing to investigate differential gene expression levels in fat body and hemocyte samples from larvae reared in high- (10 larvae per jar) and low-density (1 larva per jar) conditions; the samples exhibited density-dependent prophylaxis. Read More

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http://dx.doi.org/10.1016/j.dci.2019.02.009DOI Listing
February 2019

Folate receptor-beta expression as a diagnostic target in human & rodent nonalcoholic steatohepatitis.

Toxicol Appl Pharmacol 2019 Feb 19. Epub 2019 Feb 19.

University of Arizona, Department of Pharmacology and Toxicology, Tucson, AZ, USA. Electronic address:

Introduction: Nonalcoholic steatohepatitis (NASH) afflicts 20-36% of individuals with nonalcoholic fatty liver disease (NAFLD). A lipotoxic hepatic environment, altered innate immune signaling and inflammation are defining features of progression to NASH. Activated resident liver macrophages express folate receptor beta (FR-β) which may be an indicator of progression from steatosis to NASH. Read More

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http://dx.doi.org/10.1016/j.taap.2019.02.009DOI Listing
February 2019

Iron-oxide nanoparticles target intracellular HSP90 to induce tumor radio-sensitization.

Biochim Biophys Acta Gen Subj 2019 Feb 19. Epub 2019 Feb 19.

Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400085, India. Electronic address:

Background: Nanoparticle-based therapies have emerged as a promising approach to overcome limitations of conventional chemotherapy. Present study investigates the potential of oleic acid-functionalized iron-oxide nanoparticles (MN-OA) to enhance the radiation response of fibrosarcoma tumor and elucidates its underlying mechanism.

Methods: Various cellular and molecular assays (e. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.010DOI Listing
February 2019

Genetic strain and sex differences in a hyperoxia-induced mouse model of varying severity of bronchopulmonary dysplasia.

Am J Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pediatrics, Drexel University College of Medicine, Philadelphia, PA; St. Christopher's Hospital for Children, Philadelphia, PA. Electronic address:

Bronchopulmonary dysplasia (BPD) is a disease prevalent in preterm babies with a need for supplemental oxygen resulting in impaired lung development and dysregulated vascularization. Epidemiological studies have shown that males are more prone to BPD and have a delayed recovery compared to females for reasons unknown. Here, we tried to recapitulate mild, moderate, and severe BPD, using two different strains of mice: CD1 (outbred) and C57BL/6 (inbred), in males and females. Read More

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http://dx.doi.org/10.1016/j.ajpath.2019.01.014DOI Listing
February 2019

β-Catenin and yes-associated protein 1 cooperate in hepatoblastoma pathogenesis.

Am J Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Gynecology, Shiyan Taihe Hospital, Affiliated Hospital of Hubei University of Medicine, Shiyan, China. Electronic address:

Hepatoblastoma (HB), the most common pediatric primary liver neoplasm, show nuclear localization of β-catenin and yes-associated protein1 (YAP1) in almost 80% of the cases. Co-expression of constitutively active S127A-YAP1 and ΔN90 deletion-mutant-β-catenin (YAP1-ΔN90-β-catenin) causes HB in mice. Since heterogeneity in downstream signaling is being identified owing to mutational differences even in β-catenin gene alone, we investigated if co-expression of point-mutants of β-catenin (S33Y or S45Y) with S127A-YAP1 led to similar tumors as YAP1-ΔN90-β-catenin. Read More

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http://dx.doi.org/10.1016/j.ajpath.2019.02.002DOI Listing
February 2019

TEA domain transcription factor 4 (TEAD4) is the major mediator of Yap oncogenic activity in mouse and human hepatoblastoma.

Am J Pathol 2019 Feb 19. Epub 2019 Feb 19.

Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, CA, USA. Electronic address:

Hepatoblastoma (HB) is the most common type of pediatric liver cancer. Activation of Yes-associated protein (YAP) has been implicated in HB molecular pathogenesis. The transcriptional co-activator Yap regulates downstream gene expression through interaction with the TEA domain (TEAD) proteins. Read More

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http://dx.doi.org/10.1016/j.ajpath.2019.01.016DOI Listing
February 2019

Interleukin-6 released from differentiating human beige adipocytes improves browning.

Exp Cell Res 2019 Feb 19. Epub 2019 Feb 19.

Laboratory of Cell Biochemistry, Department of Biochemistry and Molecular Biology, University of Debrecen, Faculty of Medicine, Debrecen, Hungary; MTA-DE Stem Cells, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary. Electronic address:

Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL) -6, IL-8 and monocyte chemoattractant protein (MCP) -1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Read More

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http://dx.doi.org/10.1016/j.yexcr.2019.02.015DOI Listing
February 2019

Hepatic metabolic adaptation in a murine model of glutathione deficiency.

Chem Biol Interact 2019 Feb 19. Epub 2019 Feb 19.

Department of Environmental Health Sciences, Yale School of Public Health, New Haven, CT, 06521, USA. Electronic address:

Glutathione (GSH), the most abundant cellular non-protein thiol, plays a pivotal role in hepatic defense mechanisms against oxidative damage. Despite a strong association between disrupted GSH homeostasis and liver diseases of various etiologies, it was shown that GSH-deficient glutamate-cysteine ligase modifier subunit (Gclm)-null mice are protected against fatty liver development induced by a variety of dietary and environmental insults. The biochemical mechanisms underpinning this protective phenotype have not been clearly defined. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.02.015DOI Listing
February 2019

Quality by Design Driven Process Development of Cell Culture in Bioreactor for the Production of Foot-and-Mouth Veterinary Vaccine.

J Pharm Sci 2019 Feb 19. Epub 2019 Feb 19.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

Quality by design (QbD) principle has been established as a guideline to emphasize the understanding of the relationship of product quality with process control. Vaccine product have characteristics of security and high efficiency, but it also has features like complexity and rigorous regulatory for production. This case study describes an example of QbD driven process development for manufacturing a veterinary vaccine produced with baby hamster kidney-21 (BHK-21) cells. Read More

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http://dx.doi.org/10.1016/j.xphs.2019.02.004DOI Listing
February 2019

Validation of Human MDR1-MDCK and BCRP-MDCK Cell Lines to Improve the Prediction of Brain Penetration.

J Pharm Sci 2019 Feb 19. Epub 2019 Feb 19.

It is of great challenge to predict human brain penetration for substrates of multidrug resistance protein 1 (MDR1) and/or breast cancer resistance protein (BCRP), two major efflux transporters at blood-brain barrier. Thus, a PBPK model with the incorporation of in vitro MDR1 and BCRP transporter function data and transporter protein expression levels has been developed. As such, it is crucial to generate MDR1 and BCRP substrate data with a high fidelity. Read More

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http://dx.doi.org/10.1016/j.xphs.2019.02.005DOI Listing
February 2019

Ginsenoside Rg1 attenuates protein aggregation and inflammatory Response following cerebral ischemia and reperfusion injury.

Eur J Pharmacol 2019 Feb 19. Epub 2019 Feb 19.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun 130021 China. Electronic address:

Ginsenoside Rg1 (GS Rg1) is a glycosylated triterpenoid saponin extracted from Panax ginseng. We aim to investigate the impact of GS Rg1 on protein aggregation and inflammatory response in a cerebral ischemia/reperfusion (I/R) injury model. Rats were administered different doses of GS Rg1 (10, 20, or 40mg/kg/day) or nimodipine (1mg/kg/day) for 5 consecutive days. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.02.018DOI Listing
February 2019

Regulation of MicroRNA Machinery and Development by Interspecies S-Nitrosylation.

Cell 2019 Feb;176(5):1014-1025.e12

Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA; Harrington Discovery Institute, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA. Electronic address:

Bioactive molecules can pass between microbiota and host to influence host cellular functions. However, general principles of interspecies communication have not been discovered. We show here in C. Read More

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http://dx.doi.org/10.1016/j.cell.2019.01.037DOI Listing
February 2019

Sirt3 regulates mitophagy level to promote diabetic corneal epithelial wound healing.

Exp Eye Res 2019 Feb 19. Epub 2019 Feb 19.

From the Department of Ophthalmology, Fujian Medical University Union Hospital, Fu Zhou, China.

We aim to investigate how Sirt3 (silent mating type information regulation 2 homolog 3) promoting diabetic corneal epithelial wound healing by regulating mitophagy. The effect of HG(High Glucose, 25 mM D-glucose) on Sirt3 and LC3B(light chain 3 beta)which representing of mitophagy were investigated in TKE2 cells (a murine limbal/corneal epithelium-derived progenitor cell line) and corneal epithelium from C57BL/6J-Ins2Akita (Ins2) mice using RT-PCR and Western blotting. How overexpression of Sirt3 promoting diabetic corneal epithelial wound healing was investigated with cell migration assay、immunofluorescence、 immunofluorescence colocalization and corneal injury model. Read More

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http://dx.doi.org/10.1016/j.exer.2019.02.011DOI Listing
February 2019

The complex between a multi-crossover DNA nanostructure, PX-DNA, and T7 endonuclease I.

Biochemistry 2019 Feb 22. Epub 2019 Feb 22.

Paranemic crossover (PX) DNA is a four-stranded multi-crossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), the conclusion has not yet been unambiguous. Further investigation is needed, but will require development of new tools. Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00057DOI Listing
February 2019

Installation of Trimethyllysine Analogs on Intact Histones via Cysteine Alkylation.

Bioconjug Chem 2019 Feb 22. Epub 2019 Feb 22.

Site-specific incorporation of posttranslationally-modified amino acids into proteins, including histones, has been a subject of great interest for chemical and biochemical communities. Here, we describe a site-specific incorporation of structurally simplest trimethyllysine analogs into the position 4 of the intact histone H3 protein. An efficient alkylation of cysteine 4 of the recombinantly expressed histone H3 provides a panel of trimethyllysine analogs that differ in charge, charge density, sterics and chain length. Read More

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http://dx.doi.org/10.1021/acs.bioconjchem.9b00065DOI Listing
February 2019

Laser-Induced Graphene-PVA Composite as Robust Electrically Conductive Water Treatment Membranes.

ACS Appl Mater Interfaces 2019 Feb 22. Epub 2019 Feb 22.

Graphene nanomaterials can feature both a superb electrical conductivity and unique physical properties such as an extreme surface wettability, which is potentially applicable for many purposes including water treatment. Laser-induced graphene (LIG) is an electrically conductive three-dimensional porous carbon material prepared by direct laser writing on various polymers in ambient conditions with a CO2 laser. Low fouling LIG coatings in water technology have been reported, however, the mechanical strength and the separation properties of LIG coated membranes are limited. Read More

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http://dx.doi.org/10.1021/acsami.9b00510DOI Listing
February 2019

The metabolic abnormalities of chronic high dose glucocorticoids are not mediated by hypothalamic AgRP in male mice.

Endocrinology 2019 Feb 22. Epub 2019 Feb 22.

Division of Diabetes, Endocrinology and Gastroenterology, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK.

Glucocorticoids (Gcs) are potent and widely used medicines, but often cause metabolic side effects. A murine model of corticosterone (Cort) treatment results in increased hypothalamic expression of the melanocortin antagonist AgRP in parallel with obesity and hyperglycaemia. This study investigates how these adverse effects develop over time with particular emphasis on hypothalamic involvement. Read More

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http://dx.doi.org/10.1210/en.2019-00018DOI Listing
February 2019

Renal iron accumulation and oxidative injury with aging: effects of treatment with an iron chelator.

J Gerontol A Biol Sci Med Sci 2019 Feb 22. Epub 2019 Feb 22.

Department of Health and Human Physiology, The University of Iowa.

Dysregulation of iron metabolism in the kidney may contribute to age-related increases in renal oxidative stress and dysfunction. This study assessed the effects of short-term iron chelation on markers of iron status, oxidative stress, inflammation, and autophagy in the kidneys of old rats. Old Fischer 344 rats (24 mo) were treated with deferoxamine (DFO; 200 mg/kg, twice daily for 4. Read More

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http://dx.doi.org/10.1093/gerona/glz055DOI Listing
February 2019

Mannose-Binding Lectin 2 (MBL2) combined genotypes deficiency is associated with susceptibility for Oral Lichen Planus.

Genet Mol Biol 2019 Feb 21. Epub 2019 Feb 21.

Department of Surgical, Medical and Health Sciences, University of Trieste, Trieste, Italy.

Oral Lichen Planus (OLP) is an oral inflammatory condition, mediated by host immune system reaction, presenting basal membrane damages with inflammatory lesions in the mouth and/or skin. In this study, the role of functional polymorphisms in the MBL2 gene, encoding for Mannose-Binding Protein C (MBP-C), a member of the innate immune response and an acute-phase protein able to activate the complement cascade, was investigated to assess a possible association with OLP susceptibility in Italian patients. Two variations at the promoter region (called H/L and X/Y) and three at the first exon (at codon 52, 54, and 57) of the MBL2 gene were analyzed in 69 OLP patients and 244 healthy controls from northeastern Italy. Read More

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http://dx.doi.org/10.1590/1678-4685-GMB-2018-0015DOI Listing
February 2019
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