Search Our Scientific Publications & Authors

Curr Opin Rheumatol 2018 Dec 11. Epub 2018 Dec 11.

Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital & Harvard Medical School, Boston, USA.

Purpose Of Review: Systemic lupus erythematosus (SLE) is a multiorgan autoimmune disease characterized by IgG-autoantibodies to nuclear antigens that can deposit in the kidney and trigger lupus nephritis. Neutrophils accumulate in the kidneys of patients with proliferative LUPUS NEPHRITIS and neutrophil products and a subset of granulocytes, called low-density granulocytes (LDG) may contribute to lupus nephritis pathogenesis. Here, we will discuss recent studies implicating neutrophils in the pathogenesis of human SLE nephritis and then examine studies that provide mechanistic insights into how these cells are recruited to the glomerulus following immune complex deposition and how their products may promote lupus nephritis. Read More

J Cancer Res Ther 2018 Dec;14(Supplement):S1124-S1128

Department of Interventional Radiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

Background: Serine/threonine kinase is originally identified as an oncogene and the forkhead box transcription factor forkhead box O3a (Foxo3a) has been found to be decreased in various human cancers. In the present study, we explored the expression of Akt and FOXO3a in liver cancer cells.

Materials And Methods: Akt level was detected by Western blotting analysis. Read More

Curr Mol Med 2018 Dec 11. Epub 2018 Dec 11.

Department of Signal Transduction and Biogenic Amines, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata-700026. India.

Background: Madhuca indica belongs to the family sapotaceae, commonly known as Mahua. It is primarily known for alcoholic beverage production and is reported to have anti-inflammatory, analgesic and antipyretic properties. Madhuca indica has also been reported to be effective in several diseases. Read More

Eur J Ophthalmol 2018 Dec 12:1120672118812270. Epub 2018 Dec 12.

In-Patient Department 2, Affiliated Eye Hospital of Nanjing Medical University, Nanjing, China.

Purpose:: To compare the outcome of the sequence in the two treatments (intravitreal ranibizumab and panretinal photocoagulation) in high-risk proliferative diabetic retinopathy.

Methods:: This retrospective study included 35 patients with newly diagnosed high-risk proliferative diabetic retinopathy in 43 eyes; 18 (22 eyes) received intravitreal ranibizumab before panretinal photocoagulation (intravitreal ranibizumab+ group), while the other 17 (21 eyes) received panretinal photocoagulation before intravitreal ranibizumab (panretinal photocoagulation+ group). Each subject received three intravitreal ranibizumabs that were interleaved with three panretinal photocoagulations. Read More

Arch Orthop Trauma Surg 2018 Dec 11. Epub 2018 Dec 11.

Department of Nuclear Medicine, University Hospital, LMU Munich, Munich, Germany.

Introduction: Tenosynovial giant-cell tumor also known as pigmented villonodular synovitis (PVS) is a benign but aggressive synovial proliferative disease most often affecting the knee joint. The mainstay of therapy is surgical resection. Due to a high rate of local recurrence, radiosynoviorthesis (RSO) is used as an adjuvant method in many cases. Read More

Oncogene 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Molecular Biology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via La Masa 19, 20157, Milano, Italy.

Targeting of histone methylation has therapeutic potential in oncology. Here, we provide proof-of-principle that pharmacological inhibition of KDM5 histone-demethylases is a new strategy for the personalized treatment of HER2 breast cancer. The anti-proliferative effects of the prototype of a new class of selective KDM5-inhibitors (KDM5-inh1) are evaluated in 40 cell lines, recapitulating the heterogeneity of breast cancer. Read More

Lipids Health Dis 2018 Dec 11;17(1):280. Epub 2018 Dec 11.

University of Information Technology and Management in Rzeszow, Kielnarowa 386a, 36-020, Tyczyn, Poland.

Background: Extracts from plants, rich in antioxidants may be used as active ingredients of many preparations, mainly due to their antioxidant, regenerative and anti-aging properties. The work involved a comprehensive evaluation of the Jerusalem artichoke (Helianthus tuberosus L.) leaf and tuber extract as a multifunctional raw material. Read More

J Cardiol Cases 2012 Nov 15;6(5):e126-e129. Epub 2012 May 15.

Department of Cardiovascular Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

This case report describes an autopsy case of very late stent thrombosis, which was associated with intravascular ultrasound findings of late-acquired stent malapposition with thrombotic materials and marked positive vascular remodeling at the site of the sirolimus-eluting stent implantation. Histopathological examination showed both destructive and proliferative reactions had occurred in the same stented coronary arterial wall 44 months after sirolimus-eluting stent implantation. Long-lasting severe local inflammation beyond healing reaction, causing vessel enlargement, may be related to very late stent thrombosis. Read More

Zentralbl Chir 2018 Dec 11;143(6):609-616. Epub 2018 Dec 11.

Unfallchirurgie, Bundeswehrkrankenhaus Berlin, Deutschland.

Rinsing wounds with wound cleansing solutions has long been a recognised cornerstone in wound management as a means of removing cell debris and surface pathogens in wound exudates. In combination with surgical debridement and topical negative pressure wound therapy (NPWT), this can facilitate the intended progression from the inflammatory to the proliferative phase of wound healing. Procedures of topical negative pressure wound therapy with instillation and a defined exposure/dwell-time of topical solutions under cyclic compression and decompression with foam dressings (NPWTi-d) can remove cellular remnants and debris that may inhibit the healing process. Read More

Biomed Pharmacother 2018 Dec 8;110:582-593. Epub 2018 Dec 8.

Student Research Committee, Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address:

Skin color disorders can be caused by various factors, such as excessive exposure to sunlight, aging and hormonal imbalance during pregnancy, or taking some medications. Kojic acid (KA) is a natural metabolite produced by fungi that has the ability to inhibit tyrosinase activity in synthesis of melanin. The major applications of KA and its derivatives in medicine are based on their biocompatibility, antimicrobial and antiviral, antitumor, antidiabetic, anticancer, anti-speck, anti-parasitic, and pesticidal and insecticidal properties. Read More

J Colloid Interface Sci 2018 Dec 4;538:449-461. Epub 2018 Dec 4.

Istituto Italiano di Tecnologia, Smart Bio-Interfaces, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy; Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address:

Major obstacles to the successful treatment of gliolastoma multiforme are mostly related to the acquired resistance to chemotherapy drugs and, after surgery, to the cancer recurrence in correspondence of residual microscopic foci. As innovative anticancer approach, low-intensity electric stimulation represents a physical treatment able to reduce multidrug resistance of cancer and to induce remarkable anti-proliferative effects by interfering with Ca and K homeostasis and by affecting the organization of the mitotic spindles. However, to preserve healthy cells, it is utterly important to direct the electric stimuli only to malignant cells. Read More

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Dept. of Biomedical Engineering, Cleveland Clinic, Cleveland, OH.

Connective tissue progenitors (CTPs) are defined as the heterogeneous population of tissue-resident stem and progenitor cells that are capable of proliferating and differentiating into connective tissue phenotypes. The prevalence and variation in clonal progeny of CTPs can be characterized using a colony formation assay. However, colony assays do not directly assess the characteristics of the colony founding CTP. Read More

Arch Pharm (Weinheim) 2018 Dec 10:e1800192. Epub 2018 Dec 10.

Department of Chemistry, School of Chemical and Life Science, Jamia Hamdard, New Delhi, India.

A series of spiroisoxazoline analogues of artemisinin was synthesized by employing 1,3-dipolar cycloaddition between various in situ generated nitrile oxides and artemisitene. All the synthesized compounds were tested for their anti-proliferative and anti-malarial activities. Among the compounds tested, compound 11a was found to be potent against the HCT-15 cancer cell line with IC  = 4. Read More

Microbiol Immunol 2018 Dec 11. Epub 2018 Dec 11.

Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan.

Trichodysplasia spinulosa associated polyomavirus (TSPyV), a newly identified polyomavirus has been implicated as a causative agent of trychodysplasia spinulosa (TS), a rare proliferative skin disease in severly immunocompromised hosts. The diagnosis using monoclonal antibodies (mAbs) has been provided as a promising tool as it offers high specificity towards specific antigen. Till there is no good mAb available for diagnosis of TS disease. Read More

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

College of Life Science, Northeast Forestry University, Harbin, Heilongjiang, China.

Pancreatic stem/progenitor cells convert from a proliferative to a differentiated fate passing through proliferation cease to a resting state. However, the molecular mechanisms of cell cycle arrest are poorly understood. In this study, we demonstrated that the microRNA-124a (miR-124a) inhibited the proliferation of pancreatic progenitor cells both in vitro and ex vivo and promoted a quiescent state. Read More

J Tissue Eng Regen Med 2018 Dec 7. Epub 2018 Dec 7.

Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, USA.

Mesenchymal stem cells (MSCs) improve the osteoarthritis condition, but the fate of MSCs after intra-articular injection is unclear. We used fluorescent nanoparticles (quantum dots; QDs) to track equine MSCs (QD-MSCs) in vivo after intra-articular injection to normal and osteoarthritic joints. One week after injection of QD-MSCs, unlabeled MSCs, or vehicle we determined the presence of QD-MSCs in synovium and articular cartilage histologically. Read More

J Cell Physiol 2018 Dec 8. Epub 2018 Dec 8.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, People's Republic of China.

Long noncoding RNA (lncRNA) exerts an essential role in the pathological processes of many diseases. Our previous study found that lncRNA ATB was highly expressed in renal cell carcinoma (RCC). Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), and migration-related assays were conducted to access the regulatory effects of lncRNA ATB on proliferative and migratory capacities of RCC cells. Read More

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Lankenau Institute for Medical Research, Wynnewood, Pennsylvania.

Neovascularization in cancer or retinopathy is driven by pathological changes that foster abnormal sprouting of endothelial cells. Mouse genetic studies indicate that the stress-induced small GTPase RhoB is dispensable for normal physiology but required for pathogenic angiogenesis. In diabetic retinopathy, retinopathy of prematurity (ROP) or age-related wet macular degeneration (AMD), progressive pathologic anatomic changes and ischemia foster neovascularization are characterized by abnormal sprouting of endothelial cells. Read More

Acta Ophthalmol 2018 Dec 7. Epub 2018 Dec 7.

Department of Vitreoretinal Surgery, The Rotterdam Eye Hospital, Rotterdam, the Netherlands.

Purpose: Unremoved vitreoschisis-induced vitreous cortex remnants (VCR) are associated with macular pathology. When present on the retinal periphery, they may play a role in proliferative vitreoretinopathy and retinal detachment after vitrectomy. Existing instruments for their removal involve substantial risk of iatrogenic retinal damage. Read More

Cytometry A 2018 Dec 11. Epub 2018 Dec 11.

Metabolic Photonics Laboratory, Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, 8700 Beverly Blvd, Los Angeles, California, 90048.

Cancer cells are known to display a variety of metabolic reprogramming strategies to fulfill their own growth and proliferative agenda. With the advent of high resolution imaging strategies, metabolomics techniques, and so forth, there is an increasing appreciation of critical role that tumor cell metabolism plays in the overall breast cancer (BC) growth. In this report, we demonstrate a sensitive, flow-cytometry-based assay for rapidly assessing the metabolic phenotypes in isolated suspensions of breast cancer cells. Read More

Head Neck 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Medicine, Department of Oral Pathology, Institute of Dentistry, University of Turku, Turku, Finland.

Background: The aim was to update information on oral proliferative verrucous leukoplakia (PVL), a disease of verrucous-like lesions with high risk of malignancy, and its biomarkers.

Methods: A systematic search of literature on PVL and its biomarkers showed 22 biomarkers that were investigated in 19 papers. A meta-analysis was possible for human papillomavirus (HPV), aneuploidy, Ki-67, and p53. Read More

J Leukoc Biol 2018 Dec 11. Epub 2018 Dec 11.

Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.

The underlying mechanisms that regulate neonatal immune suppression are poorly characterized. CD31 (PECAM1) is highly expressed on neonatal lymphocytes and is a known modulator of TCR signaling. To further characterize the role of CD31 in the neonatal CTL response, 3-d and 7-d-old murine neonates were infected with influenza virus and compared to adults. Read More

Eur Rev Med Pharmacol Sci 2018 Nov;22(22):7788-7797

Department of Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.

Objective: To elucidate the biological functions of long non-coding RNA (lncRNA) SNHG7 in breast cancer (BC), and its underlying mechanism in the occurrence and progression of BC.

Patients And Methods: The expression of SNHG7 in 72 pairs of BC tissues and paracancerous tissues was detected by quantitative Real-time polymerase chain reaction (qRT-PCR). Correlation between SNHG7 expressions with pathological indicators of BC patients was analyzed. Read More

Methods Mol Biol 2019 ;1905:93-101

School of Life Science and Technology, Shanghai Tech University, Shanghai, China.

Primary hepatocytes are widely used in regenerative medicine, drug metabolism analysis, and in vitro drug screens. To overcome the shortage of liver donors, several strategies, such as differentiation of pluripotent stem cells and transdifferentiation from somatic cells, were developed to generate hepatocytes from alternative sources. Here, we describe in detail lenti-virus-based procedure for direct conversion of human fibroblasts to hepatocytes (hiHep cells) in vitro. Read More

Methods Mol Biol 2019 ;1905:71-82

Department of Developmental Biology, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.

Recent technological advances have revealed the heterogeneity of cells and tissues. Existence of heterogeneity in hepatic progenitor cells is becoming apparent by various experimental approaches, and here we describe a series of techniques to investigate the proliferative heterogeneity of these cells. We have developed a new technique by combining genetic lineage tracking and three-dimensional imaging methods. Read More

Methods Mol Biol 2019 ;1905:19-27

Oregon Stem Cell Center, Oregon Health and Science University, Portland, OR, USA.

Cholangiocytes are proliferative and are one of the sources for liver progenitor cells. Clonogenic cholangiocytes are defined as cells capable of clonally proliferating and differentiating cholangiocytes both in vitro and in vivo. In this protocol, we describe the method for isolation of primary cholangiocytes from mouse. Read More

Cardiovasc Intervent Radiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Surgery, Navicent Health Physician Group, Mercer University School of Medicine and The Medical Center Navicent Health, 1550 College Street, Macon, GA, 31207, USA.

Background: Implantation of a retrievable vena cava filter (VCF) is an effective method for preventing pulmonary embolism. Retrieval of filters, however, may be difficult due to intimal hyperplasia and inflammation in the cava wall. The transcription factor nuclear factor-kappaB (NF-κB) plays an important role in regulation of numerous genes participating in the inflammatory and proliferative responses of cells. Read More

Naunyn Schmiedebergs Arch Pharmacol 2018 Dec 7. Epub 2018 Dec 7.

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.

Liver fibrosis is a major health problem worldwide due to its serious complications including cirrhosis and liver cancer. 2-Methoxyestradiol (2-ME) is an end metabolite of estradiol with anti-proliferative, antioxidant, and anti-inflammatory activities. However, the protective role of 2-ME in liver fibrosis has not been fully investigated. Read More

Eur Heart J Cardiovasc Imaging 2018 Dec 7. Epub 2018 Dec 7.

Complications Research, Steno Diabetes Center Copenhagen, Niels Steensens Vej 2, Gentofte, Denmark.

Aims: To evaluate myocardial flow reserve (MFR) and coronary artery calcium (CAC) in persons with Type 1 diabetes with or without albuminuria and in non-diabetic controls. MFR reflects the function of large epicardial arteries and myocardial microcirculation. CAC represents structural aspects of atherosclerosis. Read More

Gigascience 2018 Dec 10. Epub 2018 Dec 10.

Department of Chemical Engineering & Biotechnology, University of Cambridge, Philippa Fawcett Drive. Cambridge CB3 0AS, United Kingdom.

Background: Cryopreservation is a routinely used methodology for prolonged storage of viable cells. The use of cryo-protective agents (CPAs) such as dimethylsulfoxide (DMSO), glycerol or trehalose is paramount to reduce cellular cryo-injury but their effectiveness is still limited. The current study focuses on establishing and modulating the proteomic and the corresponding biological profiles associated with the cryo-injury of human leukaemia (HL-60) cells cryopreserved in DMSO alone or DMSO +/- novel CPAs [e. Read More

J Mol Cell Biol 2018 Dec 11. Epub 2018 Dec 11.

Cancer Science Institute of Singapore, National University of Singapore, Singapore - 117599.

RUNX genes belong to a three-membered family of transcription factors, which are well established as master regulators of development. Of them, aberrations in RUNX3 expression are frequently observed in human malignancies primarily due to epigenetic silencing which is often overlooked. At the G1 phase of the cell cycle, RUNX3 regulates the restriction (R)-point, a mechanism that decides cell cycle entry. Read More

Rom J Morphol Embryol 2018 ;59(3):977-983

Department of Plastic Surgery, "Grigore T. Popa" University of Medicine and Pharmacy, Iaşi, Romania;

Basal cell carcinoma (BCC) is the most common type of cancer located in the periocular area. We will present the clinical case of a 63-year-old male patient who was admitted to the 2nd Clinic of Neurosurgery, "Prof. Dr. Read More

NPJ Aging Mech Dis 2019 1;5. Epub 2018 Dec 1.

Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 1-5-30 Shiba-kohen, Minato-ku, Tokyo, 105-8512 Japan.

To understand the molecular features underlying stem cell aging, we established intestinal epithelial organoids derived from both young and aged mice and investigated alterations in their senescence and epigenetic status. Senescence-related changes including accumulation of senescence-associated β-galactosidase and up-regulation of ( by DNA demethylation were observed in intestinal epithelial organoids derived from aged mice. We also demonstrated that the important stem cell marker was epigenetically silenced by trimethylation of histone H3 lysine 27, inducing suppression of Wnt signaling and a decrease of cell proliferation in organoids from aged mice. Read More

Front Pharmacol 2018 26;9:1358. Epub 2018 Nov 26.

Product Development and Advisory Services Division, Malaysian Palm Oil Board, Bandar Baru Bangi, Malaysia.

Plant-derived phytonutrients have emerged as health enhancers. Tocotrienols from the vitamin E family gained high attention in recent years due to their multi-targeted biological properties, including lipid-lowering, neuroprotection, anti-inflammatory, antioxidant, and anticancer effects. Despite well-defined mechanism of action as an anti-cancer agent, their clinical use is hampered by poor pharmacokinetic profile and low oral bioavailability. Read More

Front Cell Neurosci 2018 26;12:406. Epub 2018 Nov 26.

Department of Cellular Biology, Faculty of Biological Sciences, University of Concepcion Concepcion, Chile.

Tanycytes are hypothalamic radial glia-like cells that form the basal wall of the third ventricle (3V) where they sense glucose and modulate neighboring neuronal activity to control feeding behavior. This role requires the coupling of hypothalamic cells since transient decreased hypothalamic Cx43 expression inhibits the increase of brain glucose-induced insulin secretion. Tanycytes have been postulated as possible hypothalamic neuronal precursors due to their privileged position in the hypothalamus that allows them to detect mitogenic signals and because they share the markers and characteristics of neuronal precursors located in other neurogenic niches, including the formation of coupled networks through connexins. Read More

Microbiol Resour Announc 2018 Sep 6;7(9). Epub 2018 Sep 6.

National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.

The draft genome sequences of three strains of Lawsonia intracellularis, an obligate intracellular animal pathogen responsible for causing proliferative enteropathy, obtained from swine in different prefectures in Japan revealed the absence of a genomic island previously reported to be linked to host adaptation and to high genomic diversity, despite geographical proximity. Read More

Front Cell Dev Biol 2018 23;6:159. Epub 2018 Nov 23.

German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany.

Alzheimer's disease (AD) is the most common neurodegenerative disease and is the leading form of dementia. AD entails chronic inflammation, impaired synaptic integrity and reduced neurogenesis. The clinical and molecular onsets of the disease do not temporally overlap and the initiation phase of the cellular changes might start with a complex causativeness between chronic inflammation, reduced neural stem cell plasticity and neurogenesis. Read More

Front Cell Dev Biol 2018 23;6:158. Epub 2018 Nov 23.

Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.

Thy-1 (CD90) is a glycosylphosphatidylinositol-anchored protein (GPI-AP) with signaling properties that is abundant on mouse T cells. Upon antibody-mediated crosslinking, Thy-1 provides a T cell receptor (TcR)-like signal that is sufficient to drive CD4 T cell proliferation and differentiation into effector cells when costimulatory signals are provided by syngeneic lipopolysaccharide-matured bone marrow-derived dendritic cells. In this study, we investigated the impact of Thy-1 signaling on the production of the T helper (Th) cell subset-associated cytokines, interferon (IFN) γ, interleukin (IL)-4 and IL-17A, as well as the polarization of highly purified resting CD4 T cells into Th1, Th2, and Th17 cells. Read More

PLoS One 2018 11;13(12):e0207435. Epub 2018 Dec 11.

Medical Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India.

Purpose: To evaluate structural changes in the choroid among patients with diabetic macular edema (DME), with varying grades of diabetic retinopathy (DR), using enhance depth imaging spectral domain optical coherence tomography (EDI SD-OCT) scans.

Methods: A cross-sectional study was conducted on 82 eyes with DR and DME and 86 healthy control eyes. Eyes with DME were classified according to the severity of DR as per the international DR severity scale. Read More

Front Pharmacol 2018 23;9:1360. Epub 2018 Nov 23.

Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.

(FLL) has been widely used as a traditional Chinese medicine (TCM) for treating soreness and weakness of waist and knees. It has potential for treating OA owing to its kidney-tonifying activity with bone-strengthening effects, but there is so far no report of its anti-OA effect. This study established a rat OA model by intra-articular (IA) injection of mono-iodoacetate (1. Read More

Cancer Manag Res 2018 15;10:5735-5745. Epub 2018 Nov 15.

Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China,

Background: Hepatocellular carcinoma (HCC) is an aggressive malignancy with high incidence rate and poor prognosis. Enolase-1 (ENO1), a key glycolytic enzyme, has been implicated in the tumorigenesis of various cancers. However, its diagnostic value and clinical significance in HCC are unclear. Read More

Onco Targets Ther 2018 14;11:8153-8163. Epub 2018 Nov 14.

Department of Breast and Thyroid Surgery, Breast Cancer Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, People's Republic of China,

Background: Most breast cancers are estrogen dependent and were sensitive to endocrine therapy, and genistein (GEN) shows strong affinity with human oestrogen receptor beta (ERβ).

Purpose: The present study aimed to investigate the anticancer activity of GEN in breast cancer cell lines that constitutively expressing ERβ1 in vitro and in vivo.

Methods: MCF-7/ERβ1 and MDA-MB-231/ERβ1 cell sub-lines were established through lentiviral infection. Read More

Clin Ophthalmol 2018 14;12:2323-2329. Epub 2018 Nov 14.

Department of Ophthalmology, Toho University Sakura Medical Center, Shimoshizu, Sakura, Japan,

Purpose: To investigate the potential risk factors for neovascular glaucoma (NVG) after vitrectomy in eyes with proliferative diabetic retinopathy.

Subjects And Methods: In this retrospective, observational, comparative study, patients with proliferative diabetic retinopathy who underwent vitrectomy at Toho University Sakura Medical Center between December 2011 and November 2016 and who were followed for ≥12 months after surgery were included. Subject parameters examined included age, glycated hemoglobin (HbA1c), fasting blood glucose, administration of insulin, and estimated glomerular filtration rate. Read More

Vet World 2018 Nov 3;11(10):1376-1379. Epub 2018 Oct 3.

Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka.

Aim: The objective of this study was to describe and characterize the postmortem and histopathological findings of putative esophageal chondrosarcoma associated with .

Materials And Methods: Spirocerca-associated esophageal nodules were collected from 54 dogs at postmortem examination and were stained with hematoxylin and eosin. Of the cases examined, 15 were selected randomly for further investigation, of which 11 were classified as non-neoplastic nodules while 4 had changes reflecting a neoplastic process. Read More

Oncogene 2018 Dec 7. Epub 2018 Dec 7.

Laboratory of Molecular Biology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, via La Masa 19, 20156, Milano, Italy.

All trans-retinoic acid (ATRA) is used in the treatment of acute promyelocytic leukemia (APL) and it is a promising agent also in solid tumors. The pharmacological activity of ATRA is mediated by the ligand-activated RAR and RXR transcription factors. In the present study, we define the basal and ATRA dependent RARα interactome in a RARα-overexpressing breast cancer cellular model, identifying 28 nuclear proteins. Read More

Med Sci Monit 2018 Dec 8;24:8891-8898. Epub 2018 Dec 8.

Department of Endocrinology, Lithuanian University of Health Sciences, Kaunas, Lithuania.

BACKGROUND Diabetic retinopathy has a varied prevalence, severity, and rate of progression. The aim of this study was to determine whether the single nucleotide polymorphisms (SNPs) of the gene encoding a 135-kD centrosomal protein CEP135 rs4865047 and the gene encoding the type 2 NPY protein NPY2R rs1902491 were associated with the development of rapidly progressive proliferative diabetic retinopathy in patients with type 1 diabetes mellitus. MATERIAL AND METHODS Patients with rapidly progressive proliferative diabetic retinopathy (n=48) were included in the study group. Read More

Retina 2018 Nov 28. Epub 2018 Nov 28.

Glaucoma Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.

Purpose: To compare intraocular pressure, anterior segment optical coherence tomography, and ultrasound biomicroscopy parameters over 3 months after panretinal photocoagulation (PRP) for proliferative diabetic retinopathy after 1 of 2 sittings by conventional laser (half PRP) and a single sitting of Pattern Scan Laser (PASCAL) PRP.

Methods: This was a prospective, randomized, interventional study. All tests were performed at baseline, and at 1, 6, and 24 hours, and 1, 4, 8, and 12 weeks after PRP. Read More

Blood Adv 2018 Dec;2(23):3483-3491

Department of Systems BioMedicine, Graduate School of Medical and Dental Sciences.

Excessive and constitutive activation of nuclear factor-κB (NF-κB) leads to abnormal cell proliferation and differentiation, leading to the development of malignant tumors, including lymphoma. MicroRNA 146a (miR-146a) and miR-146b, both of which carry an identical seed sequence, have been shown to contribute to inflammatory diseases and tumors by suppressing the expression of key molecules required for NF-κB activation. However, the functional and physiological differences between miR-146a and miR-146b in disease onset have not been fully elucidated. Read More

Proc Natl Acad Sci U S A 2018 Dec 7. Epub 2018 Dec 7.

Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Centre Utrecht, 3584 CT Utrecht, The Netherlands;

The significance of cardiac stem cell (CSC) populations for cardiac regeneration remains disputed. Here, we apply the most direct definition of stem cell function (the ability to replace lost tissue through cell division) to interrogate the existence of CSCs. By single-cell mRNA sequencing and genetic lineage tracing using two Ki67 knockin mouse models, we map all proliferating cells and their progeny in homoeostatic and regenerating murine hearts. Read More

Neoplasia 2018 Dec 5;21(1):106-116. Epub 2018 Dec 5.

National Centre for Cell Science (NCCS), SP Pune University Campus, Ganeshkhind, Pune, 411007, India. Electronic address:

Glioblastoma (GBM) is one of the most aggressive and lethal types of brain tumor. Despite the advancements in conventional or targeted therapies, median survival of GBM patients is less than 12 months. Amongst various signaling pathways aberrantly activated in glioma, active Wnt/β-catenin signaling pathway is one of the crucial oncogenic players. Read More