154 results match your criteria Progres en Urologie[Journal]


Quality of Life after Reconstructive Surgery for Intestinal Fistulas.

Acta Medica (Hradec Kralove) 2018 ;61(3):103-107

Department of Gerontology and Metabolism, Teaching Hospital Hradec Králové, Charles University, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic.

Background: This retrospective clinical study would like to objectively denote a quality of life of persons afflicted by an abdominal catastrophe and managed by an extensive surgery can be almost as well conformable as those of healthy people in a similar age group.

Methods: A set of eighteen patients who were successfully surgically treated and cured enjoyed a relatively good convalescence after their surgery and returned to a satisfactory standard of life from the point of view of organ function and psychosomatic state. Statistical analysis of the data collected over a period of 1 to 6 years after this complex therapy using special questionnaire for QOL assessment SF-36 was performed. Read More

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January 2018

Artificial neural network simulation of lower limb joint angles in normal and impaired human gait.

Acta Bioeng Biomech 2018 ;20(4):43-49

Józef Piłsudski University of Physical Education in Warsaw, Faculty of Rehabilitation, Warsaw, Poland.

Purpose: Simulating the complexities of lower limb motion can be useful for orthosis or rehabilitation planning. The aim of this study was to develop an artificial neural network (ANN) able to accurately simulate the changes in the angle of the ankle, knee and hip joints during the gait cycle, then to use it to simulate the impact of a restricted range of ankle and hip joint angle changes on the progres- sion of the knee joint angle.

Methods: Thirty four young healthy students participated in the study. Read More

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January 2018
2 Reads

The complex relation between access to opioid agonist therapy and diversion of opioid medications: a case example of large-scale misuse of buprenorphine in the Czech Republic.

Harm Reduct J 2018 Dec 4;15(1):60. Epub 2018 Dec 4.

European Monitoring Centre for Drugs and Drug Addiction, Lisbon, Portugal.

Opioid agonist therapy (OAT) has been available in a standard regime in the Czech Republic since 2000. Buprenorphine is the leading medication, while methadone is available only in a few specialised centres. There is an important leakage of buprenorphine onto the illicit market, and the majority of Czech opioid users are characterised by the misuse (and injecting) of diverted buprenorphine medications. Read More

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December 2018
1 Read

An International Registry of Granulocyte Transfusions.

Transfus Med Hemother 2018 Oct 24;45(5):318-322. Epub 2018 Aug 24.

NHS Blood & Transplant/Oxford University Hospitals NHS Foundation Trust, and University of Oxford; Oxford, UK.

Introduction: Granulocyte transfusions are used to either treat or prevent life-threatening infections in neutropenic patients. Current evidence from clinical trials does not support or reject efficacy, nor guide practice.

Methods: A group of investigators have led the efforts to create an online registry to gather information on granulocyte transfusion practices from as broad a range of international settings. Read More

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October 2018
4 Reads

Strengthening Healthcare Facilities Through Water, Sanitation, and Hygiene (WASH) Improvements: A Pilot Evaluation of "WASH FIT" in Togo.

Health Secur 2018 ;16(S1):S54-S65

Andrea L. Martinsen, MPH, and Rick Gelting, PhD, are with the Emergency Response and Recovery Branch, Center for Global Health, Division of Global Health Protection, Centers for Disease Control and Prevention, Atlanta.

Water, sanitation, and hygiene (WASH) services in healthcare facilities are essential to ensure quality health care and to facilitate infection, prevention, and control practices. They are critical to responding to outbreaks and preventing healthcare-associated infections and, therefore, critical to global health security. Many healthcare facilities in low- and middle-income settings have limited WASH services. Read More

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January 2018

Proteomic analysis of dentin-enamel junction and adjacent protein-containing enamel matrix layer of healthy human molar teeth.

Eur J Oral Sci 2018 Nov 22. Epub 2018 Nov 22.

Institute of Physiology, The Czech Academy of Sciences, Prague, Czech Republic.

The dentin-enamel junction (DEJ) is the border where two different mineralized structures - enamel and dentin - meet. The protein-rich DEJ, together with the inner enamel region of mature teeth, is known to exhibit higher fracture toughness and crack growth resistance than bulk phase enamel. However, an explanation for this behavior has been hampered by the lack of compositional information for the DEJ and the adjacent enamel organic matrix (EOM). Read More

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November 2018

Benefits of pallidal stimulation in dystonia are linked to cerebellar volume and cortical inhibition.

Sci Rep 2018 Nov 21;8(1):17218. Epub 2018 Nov 21.

Department of Stereotactic and Radiation Neurosurgery, Na Homolce Hospital, Prague, Czech Republic.

Clinical benefits of pallidal deep brain stimulation (GPi DBS) in dystonia increase relatively slowly suggesting slow plastic processes in the motor network. Twenty-two patients with dystonia of various distribution and etiology treated by chronic GPi DBS and 22 healthy subjects were examined for short-latency intracortical inhibition of the motor cortex elicited by paired transcranial magnetic stimulation. The relationships between grey matter volume and intracortical inhibition considering the long-term clinical outcome and states of the GPi DBS were analysed. Read More

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November 2018
2 Reads

Less renal allograft fibrosis with valganciclovir prophylaxis for cytomegalovirus compared to high-dose valacyclovir: a parallel group, open-label, randomized controlled trial.

BMC Infect Dis 2018 Nov 15;18(1):573. Epub 2018 Nov 15.

Department of Internal Medicine I, Faculty of Medicine in Pilsen, Charles University, Czech Republic and Teaching Hospital, 30460, Pilsen, Czech Republic.

Background: Cytomegalovirus (CMV) prophylaxis may prevent CMV indirect effects in renal transplant recipients. This study aimed to compare the efficacy of valganciclovir and valacyclovir prophylaxis for CMV after renal transplantation with the focus on chronic histologic damage within the graft.

Methods: From November 2007 through April 2012, adult renal transplant recipients were randomized, in an open-label, single-center study, at a 1:1 ratio to 3-month prophylaxis with valganciclovir (n = 60) or valacyclovir (n = 59). Read More

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November 2018
3 Reads

Anterior hippocampus volume loss in narcolepsy with cataplexy.

J Sleep Res 2018 Nov 13:e12785. Epub 2018 Nov 13.

Department of Anatomy, Charles University, First Faculty of Medicine, Prague, Czech Republic.

Narcolepsy with cataplexy is a lifelong disease resulting from the loss of hypocretin neurons in the hypothalamus; structural changes are not, however, limited only to the hypothalamus. We previously revealed an overall hippocampal volume loss in narcolepsy with cataplexy. The aim of this study is to describe the volume reduction of the anterior and posterior parts of the hippocampus in patients with narcolepsy with cataplexy in comparison with a control group. Read More

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November 2018
9 Reads

Rare copy number variation in extremely impulsively violent males.

Genes Brain Behav 2018 Nov 8:e12536. Epub 2018 Nov 8.

Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic.

The genetic correlates of extreme impulsive violence are poorly understood, and there have been no studies that have systematically characterized a large group of affected individuals both clinically and genetically. We performed a genome-wide rare copy number variant (CNV) analysis in 281 males from four Czech prisons who met strict clinical criteria for extreme impulsive violence. Inclusion criteria included age ≥ 18 years, an ICD-10 diagnosis of Dissocial Personality Disorder, and the absence of an organic brain disorder. Read More

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November 2018
3 Reads

Propofol versus sevoflurane anaesthesia: effect on cognitive decline and event-related potentials.

J Clin Monit Comput 2018 Oct 31. Epub 2018 Oct 31.

Department of Anaesthesiology and Intensive Care, The University Hospital and The Faculty of Medicine in Plzen - Charles University, Alej Svobody 80, 304 60, Plzen, Czech Republic.

Postoperative cognitive dysfunction (POCD) is diagnosed in up to 30% patients after anaesthesia. The causative role of anaesthetic toxicity remains unclear. Using clinical tests, no clear-cut differences have been observed between anaesthetics so far. Read More

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October 2018
3 Reads

Genetic variation associated with chromosomal aberration frequency: A genome-wide association study.

Environ Mol Mutagen 2018 Oct 3. Epub 2018 Oct 3.

Department of Molecular Genetic Epidemiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.

Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for human biomonitoring of genotoxic exposure for decades. CA frequency in peripheral blood is a marker of cancer susceptibility. Previous studies have shown associations between genetic variants in metabolic pathway, DNA repair and major mitotic checkpoint genes and CAs. Read More

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October 2018
11 Reads
2.630 Impact Factor

Metabolism of sulfur compounds in homocystinurias.

Br J Pharmacol 2018 Oct 19. Epub 2018 Oct 19.

Department of Molecular Immunology and Toxicology, National Institute of Oncology, Budapest, Hungary.

Background And Purpose: Homocystinurias are rare genetic defects characterized by altered fluxes of sulfur compounds including homocysteine and cysteine. We explored whether the severely perturbed sulfur amino acid metabolism in patients with homocystinurias affects the metabolism of hydrogen sulfide.

Experimental Approach: We studied 10 treated patients with a block in the conversion of homocysteine to cysteine due to cystathionine β-synthase deficiency (CBSD) and six treated patients with remethylation defects (RMD) and an enhanced flux of sulfur metabolites via transsulfuration. Read More

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October 2018
1 Read

HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida.

Anat Rec (Hoboken) 2018 Oct 10. Epub 2018 Oct 10.

Institute of Anatomy, Charles University, Prague, Czech Republic.

Human natural killer (HNK)-1 antibody is an established marker of developing cardiac conduction system (CCS) in birds and mammals. In our search for the evolutionary origin of the CCS, we tested this antibody in a variety of sauropsid species (Crocodylus niloticus, Varanus indicus, Pogona vitticeps, Pantherophis guttatus, Eublepharis macularius, Gallus gallus, and Coturnix japonica). Hearts of different species were collected at various stages of embryonic development and studied to map immunoreactivity in cardiac tissues. Read More

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October 2018

Cercarial dermatitis: a systematic follow-up study of human cases with implications for diagnostics.

Parasitol Res 2018 Dec 9;117(12):3881-3895. Epub 2018 Oct 9.

Department of Parasitology, Faculty of Science, Charles University, Viničná 7, 12844, Prague 2, Czech Republic.

Cercarial dermatitis (CD) is an allergic skin disease that rises in consequence of infection by invasive stages (cercariae) of trematodes of the family Schistosomatidae. CD has been considered a re-emerging disease, human cases have been reported from all continents, and tourism-threatening outbreaks occur even in frequented recreational areas. Although the symptoms of CD are generally known, the data on immune response in human patients are sporadic and incomprehensive. Read More

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December 2018

Modulatory Effects of Levodopa on Cerebellar Connectivity in Parkinson's Disease.

Cerebellum 2018 Oct 8. Epub 2018 Oct 8.

Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany.

Levodopa has been the mainstay of symptomatic therapy for Parkinson's disease (PD) for the last five decades. However, it is associated with the development of motor fluctuations and dyskinesia, in particular after several years of treatment. The aim of this study was to shed light on the acute brain functional reorganization in response to a single levodopa dose. Read More

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October 2018
7 Reads

Involvement of K and Ca channels in hydrogen sulfide-suppressed ageing of porcine oocytes.

Biol Res 2018 Oct 5;51(1):38. Epub 2018 Oct 5.

Institute of Animal Science, Uhrineves, Prague 10, Czech Republic.

Background: Hydrogen sulfide has been shown to improve the quality of oocytes destined for in vitro fertilization. Although hydrogen sulfide is capable of modulating ion channel activity in somatic cells, the role of hydrogen sulfide in gametes and embryos remains unknown. Our observations confirmed the hypothesis that the K and L-type Ca ion channels play roles in porcine oocyte ageing and revealed a plausible contribution of hydrogen sulfide to the modulation of ion channel activity. Read More

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October 2018
5 Reads

Ghrelin Receptor Antagonism of Methamphetamine-Induced Conditioned Place Preference and Intravenous Self-Administration in Rats.

Int J Mol Sci 2018 Sep 26;19(10). Epub 2018 Sep 26.

Department of Pharmacology, Third Faculty of Medicine, Charles University, Ruska 87, 1010034 Prague, Czech Republic.

Methamphetamine abuse imposes a significant burden on individuals and society worldwide, and an effective therapy of methamphetamine addiction would provide distinguished social benefits. Ghrelin significantly participates in reinforcing neurobiological mechanisms of stimulants, including amphetamines; thus, ghrelin antagonism is proposed as a promising addiction treatment. The aim of our study was to elucidate whether the pretreatment with growth hormone secretagogue receptor (GHS-R1A) antagonist, substance JMV2959, could reduce the methamphetamine intravenous self-administration (IVSA) and the tendency to relapse, and whether JMV2959 could reduce or prevent methamphetamine-induced conditioned place preference (CPP) in rats. Read More

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September 2018
15 Reads

Peripapillary microcirculation in Leber hereditary optic neuropathy.

Acta Ophthalmol 2018 Sep 26. Epub 2018 Sep 26.

Department of Ophthalmology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.

Purpose: In this prospective observational comparative case series, we aimed to study the peripapillary capillary network with spectral-domain optical coherence tomography angiography (OCT-A) in Leber hereditary optic neuropathy (LHON).

Methods: Twelve eyes of six individuals, of these three males (five eyes) after clinical onset of visual impairment were imaged by OCT-A with scans centred on optic discs. Control group consisted of 6 eyes with no visual impairment. Read More

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September 2018
8 Reads

Effects of continuous positive airway pressure on neurocognitive and neuropsychiatric function in obstructive sleep apnea.

J Sleep Res 2018 Sep 20:e12761. Epub 2018 Sep 20.

Prague College of Psychosocial Studies, Prague, Czech Republic.

The aim of this study was to determine the neurocognitive and neuropsychiatric effects of continuous positive airway pressure treatment on patients with obstructive sleep apnea. This cross-sectional, prospective, observational study included 126 patients with sleep apnea. The following tests were performed: the Montreal Cognitive Assessment for the evaluation of cognitive impairment, the Beck Depression Inventory, and the State-Trait Anxiety Inventory, together with the Epworth Sleepiness Scale for the evaluation of neuropsychiatric symptoms and a person's general level of daytime sleepiness. Read More

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September 2018
2 Reads

3D electroanatomical mapping is less sensitive to atrial remodeling in estimation of true left atrial volume than echocardiography.

BMC Med Imaging 2018 09 18;18(1):32. Epub 2018 Sep 18.

2nd Department of Medicine - Department of Cardiovascular Medicine, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 2, 128 08, Prague, Czech Republic.

Background: Left atrial (LA) enlargement has been identified as a predictor of worse clinical outcome after catheter ablation for atrial fibrillation (AF). We investigated the correspondence of LA size parameters assessed by echocardiography, CT and 3D electroanatomical mapping in patients with AF treated by catheter ablation.

Methods: We analyzed echocardiographic LA volume measurements by disc summation method (LAV), computed tomography (LAV) and 3D electroanatomical mapping (LAV) in 100 pts. Read More

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September 2018
5 Reads

Multidisciplinary approach in the treatment of spinal dural arteriovenous fistula-results of endovascular and surgical treatment.

Acta Neurochir (Wien) 2018 Dec 18;160(12):2439-2448. Epub 2018 Sep 18.

Department of Neurology, Comprehensive Stroke Center, Faculty of Medicine and University Hospital, Charles University in Prague, Hradec Králové, Czech Republic.

Background: The results of treatment for spinal dural arteriovenous fistula (SDAVF) have been controversial. The goal of this study was to compare results of endovascular and surgical treatments to contribute to determining an optimal treatment strategy.

Methods: A retrospective analysis of the set of 24 SDAVF patients (11 in the endovascular and 13 in the surgical group) was performed. Read More

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December 2018

Schnyder corneal dystrophy and associated phenotypes caused by novel and recurrent mutations in the UBIAD1 gene.

BMC Ophthalmol 2018 Sep 17;18(1):250. Epub 2018 Sep 17.

Research Unit for Rare Diseases, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 128 08, Prague 2, Czech Republic.

Background: The purpose of this study was to identify the genetic cause and describe the clinical phenotype of Schnyder corneal dystrophy (SCD) in six unrelated probands.

Methods: We identified two white Czech, two white British and two South Asian families with a clinical diagnosis of SCD. Ophthalmic assessment included spectral domain optical coherence tomography (SD-OCT) of one individual with advanced disease, and SD-OCT and confocal microscopy of a child with early stages of disease. Read More

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September 2018
2 Reads

Deep Airway Inflammation and Respiratory Disorders in Nanocomposite Workers.

Nanomaterials (Basel) 2018 Sep 16;8(9). Epub 2018 Sep 16.

UMass, Lowell, Department of Biomedical and Nutritional Sciences, Zuckerberg College of Health Sciences, Lowell, MA 01854, USA.

Thousands of researchers and workers worldwide are employed in nanocomposites manufacturing, yet little is known about their respiratory health. Aerosol exposures were characterized using real time and integrated instruments. Aerosol mass concentration ranged from 0. Read More

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September 2018
11 Reads

lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer.

Int J Mol Sci 2018 Sep 8;19(9). Epub 2018 Sep 8.

Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, 32300 Pilsen, Czech Republic.

Although colorectal cancer (CRC) is the third most frequent cause of cancer related death in Europe, clinically relevant biomarkers for therapy guidance and prognosis are insufficiently reliable. Long non-coding RNAs (lncRNAs) are RNAs over 200 nucleotides long that are not translated into proteins but can influence biological processes. There is emerging evidence for their involvement in solid cancer as oncogenes, tumour suppressors or regulators of cell proliferation and metastasis development. Read More

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September 2018
3 Reads

Potential loss of prognostic significance of minimal residual disease assessment after R-CHOP-based induction in elderly patients with mantle cell lymphoma in the era of rituximab maintenance.

Hematol Oncol 2018 Dec 13;36(5):773-778. Epub 2018 Sep 13.

First Medical Department, Charles University General Hospital in Prague, Czech Republic.

Rituximab maintenance (RM) prolongs survival of elderly patients with mantle cell lymphoma (MCL). Persistent minimal residual disease (MRD) after induction repeatedly correlated with shorter progression-free survival (PFS). However, none of the published studies analyzed patients treated with RM. Read More

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December 2018
1 Read

Mutational screening of inverted formin 2 in adult-onset focal segmental glomerulosclerosis or minimal change patients from the Czech Republic.

BMC Med Genet 2018 Aug 20;19(1):147. Epub 2018 Aug 20.

Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.

Background: Mutations in INF2 are frequently responsible for focal segmental glomerulosclerosis (FSGS), which is a common cause of end stage renal disease (ESRD); additionally, they are also connected with Charcot-Marie-Tooth neuropathy. INF2 encodes for inverted formin 2. This protein participates in regulation of the dynamics of the actin cytoskeleton, involving not only the polymerisation, but also the depolymerisation of filaments. Read More

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August 2018
2 Reads

Note on the use of different approaches to determine the pore sizes of tissue engineering scaffolds: what do we measure?

Biomed Eng Online 2018 Aug 17;17(1):110. Epub 2018 Aug 17.

Institute of Dental Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Kateřinská 32, 128 01, Prague 2, Czech Republic.

Background: Collagen-based scaffolds provide a promising option for the treatment of bone defects. One of the key parameters of such scaffolds consists of porosity, including pore size. However, to date, no agreement has been found with respect to the methodology for pore size evaluation. Read More

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August 2018
5 Reads

Antimicrobial efficiency and stability of two decontamination solutions.

Cell Tissue Bank 2018 Dec 30;19(4):581-589. Epub 2018 Jul 30.

Laboratory of the Biology and Pathology of the Eye, Department of Paediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.

Two decontamination solutions, commercially produced BASE•128 and laboratory decontamination solution (LDS), with analogous content of antibiotic and antimycotic agents, were compared in their antimicrobial efficiency and stability (pH and osmolarity). Both solutions were compared immediately after thawing aliquots frozen for 1, 3 or 6 months. Agar well diffusion method was used to test their antimicrobial efficiency against five human pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Enterococcus faecalis. Read More

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December 2018

Effects of branched-chain amino acids on muscles under hyperammonemic conditions.

J Physiol Biochem 2018 Nov 30;74(4):523-530. Epub 2018 Jul 30.

Department of Physiology, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic.

The aim was to determine the effects of enhanced availability of branched-chain amino acids (BCAAs; leucine, isoleucine, and valine) on ammonia detoxification to glutamine (GLN) and protein metabolism in two types of skeletal muscle under hyperammonemic conditions. Isolated soleus (SOL, slow-twitch) and extensor digitorum longus (EDL, fast-twitch) muscles from the left leg of white rats were incubated in a medium with 1 mM ammonia (NH3 group), BCAAs at four times the concentration of the controls (BCAA group) or high levels of both ammonia and BCAA (NH3 + BCAA group). The muscles from the right leg were incubated in basal medium and served as paired controls. Read More

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November 2018
1 Read

Vasa vasorum: an old term with new problems.

Surg Radiol Anat 2018 Oct 18;40(10):1159-1164. Epub 2018 Jul 18.

Department of Anatomy, Third Faculty of Medicine, Charles University, Ruska 87, 100 00, Prague 10, Czech Republic.

Purpose: The aim of the study was to describe the origin of the Latin anatomical term vasa vasorum and its role in current medical research and to present examples of grammatical errors in its use.

Methods: Literary searches oriented on the term vasa vasorum were used to identify publications using it in the medical literature from the seventeenth century up to the present.

Results: The Latin term vasa vasorum was introduced by Ludwig in 1739. Read More

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October 2018
10 Reads

Exhaled Breath Condensate: Pilot Study of the Method and Initial Experience in Healthy Subjects.

Acta Medica (Hradec Kralove) 2018;61(1):8-16

2nd Department of Internal Medicine - Gastroenterology, Charles University, Faculty of Medicine in Hradec Králové, University Hospital Hradec Králové, Czech Republic.

Analysis of Exhaled breath condensate (EBC) is a re-discovered approach to monitoring the course of the disease and reduce invasive methods of patient investigation. However, the major disadvantage and shortcoming of the EBC is lack of reliable and reproducible standardization of the method. Despite many articles published on EBC, until now there is no clear consensus on whether the analysis of EBC can provide a clue to diagnosis of the diseases. Read More

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November 2018

Heartfelt sepsis: microvascular injury due to genomic storm.

Authors:
Jacek Hawiger

Kardiol Pol 2018 5;76(8):1203-1216. Epub 2018 Jul 5.

Vanderbilt University Medical Center.

Sepsis is one of the ten leading causes of death in developed and developing countries. In the United States, sepsis mortality approaches that of acute myocardial infarction and exceeds deaths from stroke. Neonates and the elderly are the most vulner-able patients, with these groups suffering from the highest sepsis mortality. Read More

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July 2018
2 Reads

Bilineal inheritance of pathogenic PKD1 and PKD2 variants in a Czech family with autosomal dominant polycystic kidney disease - a case report.

BMC Nephrol 2018 Jul 4;19(1):163. Epub 2018 Jul 4.

Institute of Biology and Medical Genetics, First Faculty of Medicine Charles University and General University Hospital in Prague, Albertov 4, 128 00, Prague, Czech Republic.

Background: Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary renal disorder, leading to end stage renal failure and kidney transplantation in its most serious form. The severity of the disease's manifestation depends on the genetic determination of ADPKD. The huge variability of different phenotypes (even within a single family) is not only modulated by the two main ADPKD genes (PKD1 and PKD2) but also by modifier genes and the whole genetic background. Read More

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July 2018
3 Reads

Aldehyde dehydrogenase 2 polymorphism affects the outcome of methanol poisoning in exposed humans.

Clin Genet 2018 Nov 18;94(5):445-449. Epub 2018 Jul 18.

Toxicological Information Center, Department of Occupational Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.

As the susceptibility of humans to xenobiotics often depends on genetic factors, we assumed that ADH1B and ALDH2 genetic variants may affect susceptibility to the acute methanol exposure. To evaluate the role of genetic variants of enzymes involved in methanol catabolism in humans, we analysed ADH1B (rs1229984) and ALDH2 (rs441) polymorphisms in 50 adults who survived acute methanol poisoning, 246 individuals with alcoholic liver cirrhosis, and in 545 healthy controls. GG homozygotes of ADH1B were more common among methanol-poisoned patients (98%) and among patients with alcoholic liver cirrhosis (98%) than among healthy controls (90%) (P = 0. Read More

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November 2018
1 Read

Endometriosis - insights into a multifaceted entity.

Folia Histochem Cytobiol 2018 ;1(2):61-82

Department of Morphofunctional Sciences I - Histology, "Grigore T. Popa" University of Medicine and Pharmacy, Iaşi, Romania.

Firstly described at the end of nineteenth century, endometriosis remains an enigmatic disease, from etio-pathogenesis to specific markers of diagnosis and its ability to associate with malignancies. Our review has been designed from a historical perspective and steps up to an updated understanding of the disease, facilitated by relatively recent molecular and genetic progresses. Although the histopathological diagnosis is relatively simple, the therapy is difficult or ineffective. Read More

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November 2018
1 Read

Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes.

J Assist Reprod Genet 2018 Aug 13;35(8):1407-1417. Epub 2018 Jun 13.

International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.

Purpose: The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15.

Methods: This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Read More

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August 2018
13 Reads

Conservative management of endometrial cancer: a survey amongst European clinicians.

Arch Gynecol Obstet 2018 Aug 26;298(2):373-380. Epub 2018 Jun 26.

Department Gynecological Oncology, Poole NHS Trust, Poole, UK.

Objective: To investigate differences and similarities in the clinical approach of young clinicians managing women with endometrial cancer (EC) conservatively.

Methods: A web-based survey was carried out. A platform of the European Network of Young Gynaecological Oncologists (ENYGO) database was used. Read More

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August 2018
8 Reads

Mastoid foramen, mastoid emissary vein and clinical implications in neurosurgery.

Acta Neurochir (Wien) 2018 Jul 20;160(7):1473-1482. Epub 2018 May 20.

Department of Anatomy, Second Faculty of Medicine, Charles University, U nemocnice 3, Praha 2, 12800, Prague, Czech Republic.

Background: Mastoid emissary vein is especially important from the neurosurgical point of view, because it is located in variable number in the area of the occipitomastoid suture and it can become a source of significant bleeding in surgical approaches through the mastoid process, especially in retrosigmoid craniotomy, which is used for approaches to pathologies localized in the cerebellopontine angle. Ideal imaging method for diagnosis of these neglected structures when planning a surgical approach is high-resolution computed tomography. The aim of this work was to provide detailed information about this issue. Read More

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July 2018
19 Reads

A pedometer-based walking intervention with and without email counseling in general practice: a pilot randomized controlled trial.

BMC Public Health 2018 May 16;18(1):635. Epub 2018 May 16.

Faculty of Physical Education and Sport, Charles University, Jose Martiho 31, 162 52, Prague 6, Czech Republic.

Background: General practitioners play a fundamental role in combatting the current epidemic of physical inactivity, and pedometer-based walking interventions are able to increase physical activity levels of their patients. Supplementing these interventions with email counseling driven by feedback from the pedometer has the potential to further improve their effectiveness but it has to be yet confirmed in clinical trials. Therefore, the aim of our pilot randomized controlled trial is to evaluate the feasibility and potential efficacy of future trials designed to assess the additional benefit of email counseling added to a pedometer-based intervention in a primary care setting. Read More

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Gene Expression Analysis of Immunomagnetically Enriched Circulating Tumor Cell Fraction in Castration-Resistant Prostate Cancer.

Mol Diagn Ther 2018 May 3. Epub 2018 May 3.

Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital and First Faculty of Medicine, Charles University in Prague, U Nemocnice 2, 12808, Prague, Czech Republic.

Background: Molecular characterization of tumors could be a key to therapeutic decision-making with regards to targeted therapies in castration-resistant prostate cancer (CRPC). A convenient solution may be non-invasive liquid biopsy testing of circulating tumor cells (CTCs). For this reason, CTC-enriched samples obtained by immunomagnetic separation (AdnaTest) were studied as a source material for high-throughput gene expression analysis using BioMark™. Read More

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May 2018
2 Reads

[Quality of Life After High-dose Brachytherapy in Patients with Early Oral Carcinoma].

Klin Onkol 2018 ;31(2):125-129

Background: The prevalence of head and neck tumors has continually increased over the past decades, which is a serious medical and social problem. This study retrospectively assessed the quality of life of patients with early-stage oral cavity tumors after high-dose rate (HDR) brachytherapy.

Material And Methods: Quality of life was evaluated in 14 patients with early-onset oral cavity tumors who underwent HDR brachytherapy at the Department of Oncology and Radiotherapy, University Hospital Hradec Kralove. Read More

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January 2018

Dying in hospital: Qualitative study among caregivers of terminally ill patients who are transferred to the emergency department.

Presse Med 2018 May 2;47(5):e83-e90. Epub 2018 Apr 2.

CHRU de Besançon, service de soins palliatifs, 25000 Besançon, France; CHRU de Besançon, centre hospitalier régional universitaire de Besançon, CIC 1431 Inserm, équipe « Éthique et progrès médical », 25000 Besançon, France; Université Bourgogne-Franche-Comté, neuroscience intégratives et cliniques, 25000 Besançon, France.

Introduction: Most people in France die in the hospital, even though a majority would like to die at home. These end-of-life hospital admissions sometimes occur in the emergency setting, in the hours preceding death.

Objective: To understand the motives that incite main natural caregivers to transfer terminally ill patients at the end of life to the emergency department. Read More

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May 2018
2 Reads

Genotyping of Mycobacterium tuberculosis using whole genome sequencing.

Folia Microbiol (Praha) 2018 Sep 17;63(5):537-545. Epub 2018 Mar 17.

Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic.

Tuberculosis (TB) is considered one of the most serious infectious diseases worldwide. Effective control of tuberculosis infection involves multiple steps, such as reliable detection, treatment, an epidemiological control as a part of case management, and further surveillance and monitoring of TB spread in the human population. Due to the accelerating advances in molecular biology, especially in DNA sequencing, in the past decade, the application of these methods has become crucial for TB evolution studies, differentiation of Mycobacterium tuberculosis genotypes, and their distribution. Read More

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September 2018
1.150 Impact Factor

Management of multiple sclerosis patients in central European countries: current needs and potential solutions.

Ther Adv Neurol Disord 2018 22;11:1756286418759189. Epub 2018 Feb 22.

Department of Neurology and Center of Clinical Neuroscience, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic.

Multiple sclerosis (MS) experts in Europe are facing rapidly rising demands of excellence due to the increasing complexity of MS therapy and management. A central European expert board of MS experts met to identify needs and obstacles with respect to raising quality of MS care in central and Eastern European countries. There are substantial variations across countries regarding delivery of care and its cost structure, as well as access to treatment. Read More

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February 2018
23 Reads

Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp.

Parasit Vectors 2018 03 6;11(1):142. Epub 2018 Mar 6.

Department of Parasitology, Faculty of Science, Charles University, Viničná 7, 12844, Prague 2, Czech Republic.

Background: Cysteine peptidases of clan CA, family C1 account for a major part of proteolytic activity in the haematophagous monogenean Eudiplozoon nipponicum. The full spectrum of cysteine cathepsins is, however, unknown and their particular biochemical properties, tissue localisation, and involvement in parasite-host relationships are yet to be explored.

Methods: Sequences of cathepsins L and B (EnCL and EnCB) were mined from E. Read More

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March 2018
3 Reads

The Magistral Phage.

Viruses 2018 02 6;10(2). Epub 2018 Feb 6.

Federal Agency for Medicines and Health Products, Place Victor Horta 40/40, 1060 Brussels, Belgium.

Since time immemorial, phages-the viral parasites of bacteria-have been protecting Earth's biosphere against bacterial overgrowth. Today, phages could help address the antibiotic resistance crisis that affects all of society. The greatest hurdle to the introduction of phage therapy in Western medicine is the lack of an appropriate legal and regulatory framework. Read More

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February 2018
7 Reads

Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?

J Mater Sci Mater Med 2018 Feb 1;29(3):20. Epub 2018 Feb 1.

Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, Pilsen, Czech Republic.

Collagen composite scaffolds have been used for a number of studies in tissue engineering. The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling. The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo. Read More

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February 2018
5 Reads

MicroRNA expression in SMARCB1/INI1-deficient sinonasal carcinoma: a clinicopathological and molecular genetic study.

Virchows Arch 2018 May 11;472(5):871-875. Epub 2018 Jan 11.

The Fingerland Department of Pathology, Faculty of Medicine and University Hospital, Charles University, Sokolska 581, 500 05, Hradec Kralove, Czech Republic.

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May 2018
12 Reads