5 results match your criteria Process Biochemistry [Journal]

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Transfection in perfused microfluidic cell culture devices: A case study.

Process Biochem 2017 Aug;59(Pt B):297-302

Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, London WC1H 0AH, United Kingdom.

Automated microfluidic devices are a promising route towards a point-of-care autologous cell therapy. The initial steps of induced pluripotent stem cell (iPSC) derivation involve transfection and long term cell culture. Integration of these steps would help reduce the cost and footprint of micro-scale devices with applications in cell reprogramming or gene correction. Read More

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http://dx.doi.org/10.1016/j.procbio.2016.09.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615110PMC
August 2017
8 Reads

Adipogenic Differentiation of Human Adipose-Derived Stem Cells Grown as Spheroids.

Process Biochem 2017 Aug 6;59:312-320. Epub 2017 Feb 6.

Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, 2500 N State Street, Jackson, MS.

Understanding the process of adipogenesis is critical if suitable therapeutics for obesity and related metabolic diseases are to be found. The current study presents proof of feasibility of creating a 3-D spheroid model using human adipose-derived stem cells (hASCs) and their subsequent adipogenic differentiation. hASC spheroids were formed atop an elastin-like polypeptide-polyethyleneimine (ELP-PEI) surface and differentiated using an adipogenic cocktail. Read More

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http://dx.doi.org/10.1016/j.procbio.2017.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617119PMC
August 2017
15 Reads

Single-Step Purification of Ovalbumin from Egg White Using Aqueous Biphasic Systems.

Process Biochem 2016 Jun;51(6):781-791

CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.

The ability of aqueous biphasic systems (ABS) composed of polyethylene glycols of different molecular weights (PEG 400, 600 and 1000) and buffered aqueous solutions of potassium citrate/citric acid (pH = 5.0 - 8.0) to selectively extract ovalbumin from egg white was here investigated. Read More

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http://dx.doi.org/10.1016/j.procbio.2016.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5026300PMC
June 2016
9 Reads

Extraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids.

Process Biochem 2015 Jul;50(7):1158-1166

CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.

Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. Read More

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http://dx.doi.org/10.1016/j.procbio.2015.03.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321599PMC
July 2015
29 Reads

Optimization of the Production of the Lantibiotic Mutacin 1140 in Minimal Media.

Process Biochem 2010 Jul;45(7):1187-1191

Mississippi State University, Department of Biological Sciences, Mississippi State, Mississippi 39762.

Mutacin 1140 is produced by Streptococcus mutans and belongs to the type A lantibiotic family. Experiments were done to optimize production of mutacin 1140 in minimal media enabling a more cost efficient downstream purification method. The development of a small volume fermentation method enabled a rapid screen of several variables in a standard shaking incubator. Read More

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http://dx.doi.org/10.1016/j.procbio.2010.03.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2918913PMC
July 2010
9 Reads
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