Biosens Bioelectron 2022 Aug 6;209:114165. Epub 2022 Apr 6.
Department of Chemistry ''Giacomo Ciamician'', University of Bologna, Via Selmi 2, 40126, Bologna, Italy; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31, 98168, Messina, Italy; Institute for Microelectronics and Microsystems, Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada n.5, I-95121 Catania, Italy. Electronic address:
Detection of nucleic acids is crucial in many medical applications, and in particular for monitoring infectious diseases, as it has become perfectly clear after the pandemic infection of COVID-19. In this context, the development of innovative detection methods based on signal-amplification rather than analyte-amplification represents a significant breakthrough compared to existing PCR-based methodologies, allowing the development of new nucleic acid detection technologies suitable to be integrated in portable and low-cost sensor devices while keeping high sensitivities, thus enabling massive diagnostic screening. In this work, we present a novel molecular sensor for the ultrasensitive PCR-free detection of Hepatitis B Virus (HBV) based on electrochemiluminescence (ECL). Read More