51,953 results match your criteria Pneumonia Fungal


Performance of the Cepheid Methicillin-Resistant Staphylococcus aureus/S. aureus Skin and Soft Tissue Infection PCR Assay on Respiratory Samples from Mechanically Ventilated Patients for S. aureus Screening during the Phase 2 Double-Blind SAATELLITE Study.

J Clin Microbiol 2022 Jun 27:e0034722. Epub 2022 Jun 27.

Early Vaccines & Immune Therapies, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, Maryland, USA.

We investigated the performance of the Xpert methicillin-resistant Staphylococcus aureus (MRSA)/S. aureus skin and soft tissue (SSTI) quantitative PCR (qPCR) assay in SAATELLITE, a multicenter, double-blind, phase 2 study of suvratoxumab, a monoclonal antibody (MAb) targeting S. aureus alpha-toxin, for reducing the incidence of S. Read More

View Article and Full-Text PDF

Updated Living Systematic Review and Meta-analysis of Controlled Trials of Mesenchymal Stromal Cells to Treat COVID-19: A Framework for Accelerated Synthesis of Trial Evidence for Rapid Approval-FASTER Approval.

Stem Cells Transl Med 2022 Jun 27. Epub 2022 Jun 27.

Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.

Background: Mesenchymal stromal cells (MSCs) may reduce mortality in patients with COVID-19; however, early evidence is based on few studies with marked interstudy heterogeneity. The second iteration of our living systematic review and meta-analysis evaluates a framework needed for synthesizing evidence from high-quality studies to accelerate consideration for approval.

Methods: A systematic search of the literature was conducted on November 15, 2021, to identify all English-language, full-text, and controlled clinical studies examining MSCs to treat COVID-19 (PROSPERO: CRD42021225431). Read More

View Article and Full-Text PDF

Updated estimated incidence and prevalence of serious fungal infections in Trinidad and Tobago.

IJID Reg 2021 Dec 27;1:34-40. Epub 2021 Sep 27.

Manchester Fungal Infection Group, The University of Manchester and Manchester Academic Health Science Centre, Manchester, UK.

Objective: : To estimate the incidence and prevalence of serious fungal infections in Trinidad and Tobago (T&T), with a population of 1 394 973 million.

Methods: : The medical literature was searched to obtain published data on the incidence and prevalence of fungal infections in the Caribbean. If data were unavailable, estimations were performed using the frequencies of fungal infection in populations at risk. Read More

View Article and Full-Text PDF
December 2021

Chemotaxonomy and Antibacterial Activity of the Extracts and Chemical Constituents of Hiern. (Rubiaceae).

Biomed Res Int 2022 15;2022:7856305. Epub 2022 Jun 15.

Research Unit of Applied and Environmental Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

The use of natural products for medicinal purposes is becoming more and more common nowadays, as evidenced by the presence in plants of secondary metabolites with different potentials such as antioxidant and antibacterial properties. We evaluated in this work the antimicrobial activities of the extracts and some isolated compounds from the seeds of Hiern. (Rubiaceae), a Cameroonian medicinal plant traditionally used to cure microbial infections. Read More

View Article and Full-Text PDF

Pneumocystis Infection in Two Patients Treated with Both Immune Checkpoint Inhibitor and Corticoids.

J Immunother Precis Oncol 2020 Feb 10;3(1):27-30. Epub 2020 Feb 10.

Department of Dermatology, Erasme Hospital, ULB, Brussels, Belgium.

The introduction of immune checkpoint inhibitor (ICI) targeting cytotoxic T-lymphocyte-associated antigen-4 and programmed cell death receptor 1 has dramatically improved clinical outcome for cancer patients. Nevertheless, this treatment can be associated with immune-related adverse events (irAEs) which sometimes need management with prolonged immune suppression. In order to analyze the risk of pneumonia (PJP) in this population, all PJP cases at our oncological hospital between 2004 and 2019 were searched. Read More

View Article and Full-Text PDF
February 2020

Dynamic Python-Based Method Provides Quantitative Analysis of Intercellular Junction Organization During Infection of the Respiratory Epithelium.

Front Cell Infect Microbiol 2022 10;12:865528. Epub 2022 Jun 10.

Department of Biological Sciences, San Jose State University, San Jose, CA, United States.

Many respiratory pathogens compromise epithelial barrier function during lung infection by disrupting intercellular junctions, such as adherens junctions and tight junctions, that maintain intercellular integrity. This includes , a leading cause of pneumonia, which can successfully breach the epithelial barrier and cause severe infections such as septicemia and meningitis. Fluorescence microscopy analysis on intercellular junction protein manipulation by respiratory pathogens has yielded major advances in our understanding of their pathogenesis. Read More

View Article and Full-Text PDF

Analysis of Microbiological and Clinical Characteristics of Bacterial Infection in Patients with Pulmonary Infection.

Authors:
Taimei Duan

Comput Intell Neurosci 2022 16;2022:5607358. Epub 2022 Jun 16.

The First People's Hospital of Chongqing Liangjiang New Area, Chongqing 401121, China.

Objective: Using data investigation, the microbiology of bacterial infection in patients with pulmonary infection was discussed, and its clinical characteristics were analyzed.

Methods: The clinical data of 160 patients with pulmonary infection in our hospital from March 2019 to March 2021 were collected and analyzed. Blood samples were collected and cultured, and the pathogens were identified. Read More

View Article and Full-Text PDF

Early outcomes in adults hospitalized with severe SARS-CoV-2 infection receiving tocilizumab.

Med Clin (Engl Ed) 2022 Jun 18;158(11):509-518. Epub 2022 Jun 18.

Infectious Diseases Department, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, Barcelona, Spain.

Background: Modulation of the immune system to prevent lung injury is being widely used against the new coronavirus disease (COVID-19). The primary endpoint was mortality at 7 days after tocilizumab administration. Secondary endpoints were admission to the intensive care unit, development of ARDS and respiratory insufficiency among others. Read More

View Article and Full-Text PDF

Disseminated Coccidioidomycosis in an Immunocompetent Male Who Lived in an Endemic Region in the Remote Past: A Case Report.

Cureus 2022 May 23;14(5):e25249. Epub 2022 May 23.

Internal Medicine, Michigan State University, East Lansing, USA.

Coccidioidomycosis is an endemic illness suspected in patients who live in or have recently traveled to an endemic area. Disseminated disease is less frequent and is almost always seen in the presence of risk factors such as immunosuppression. We present a case of disseminated coccidioidomycosis with a delayed presentation in a young immunocompetent male. Read More

View Article and Full-Text PDF

Comparison of pneumonia features in children caused by SARS-CoV-2 and other viral respiratory pathogens.

Pediatr Pulmonol 2022 Jun 26. Epub 2022 Jun 26.

Pediatrics Department. Hospital Universitario Infanta Sofía. Pediatrics Research Group. Universidad Europea de Madrid, Spain. Pediatric Research and Clinical Trials Unit (UPIC). Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain. Fundación de Investigación Biomédica Hospital 12 de Octubre. RITIP (Translational Research Network in Paediatric Infectious Diseases).

View Article and Full-Text PDF

Biomarkers for iron metabolism among patients hospitalized with community-acquired pneumonia caused by infection with SARS-CoV-2, bacteria, and influenza.

APMIS 2022 Jun 25. Epub 2022 Jun 25.

Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.

Background: Ferritin, the central iron storage protein, has attracted attention as a biomarker of severe COVID-19. Few studies have investigated regulators of iron metabolism in the context of COVID-19. The aim was to evaluate biomarkers for iron metabolism in the acute phase response to community-acquired pneumonia (CAP) caused by SARS-CoV-2 compared to CAP caused by bacteria or influenza virus in hospitalized patients. Read More

View Article and Full-Text PDF

BCL10 loss-of-function novel mutation leading to atypical severe combined immunodeficiency.

Clin Immunol 2022 Jun 21:109067. Epub 2022 Jun 21.

Department of Clinical Genetics, Sultan Qaboos University Hospital, Muscat, Oman; Department of Genetics, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman.

Background: Severe combined immunodeficiency (SCID) is characterized by severe, early-onset infection in infants. B-cell lymphoma/leukemia (BCL) 10 defects causing SCID have been reported previously in two patients.

Material & Methods: A seven-month-old female infant was admitted with bilateral pneumonia requiring ventilatory support. Read More

View Article and Full-Text PDF

Bloodstream infection in hospital therapeutic patients.

Klin Lab Diagn 2022 Jun;67(6):355-361

G.N. Gabrichevskii Moscow research institute for epidemiology and microbiology for Rospotrebnadzor.

Bloodstream infection (BI) is the cause of high mortality. Hospital bloodstream infection (HBI) complicates hemodialysis, pneumonia, oncohematological diseases. Positive hemoculture obtaining depends on the volume of blood inoculation, the number of blood samples, the incubation time. Read More

View Article and Full-Text PDF

Multifocal lymphadenopathies with polyclonal reactions primed after EBV infection in a mRNA-1273 vaccine recipient.

Swiss Med Wkly 2022 05 23;152:w30188. Epub 2022 May 23.

Service of Infectious Diseases, University Hospital Centre (CHUV), Lausanne, Switzerland.

We report a case of recurrent tender, multifocal lymphadenopathies associated with B-symptoms, clinically mimicking lymphoma in a mRNA-1273 vaccine recipient after a recent Epstein-Barr virus (EBV) infection. In the lymph node biopsy, monocytoid B-cell hyperplasia, TH2 (GATA3+) predominance, and hyperplasia of interferon-gamma-producing plasmacytoid dendritic cells were observed along with sustained neutralising antibody production against SARS-CoV-2 wild-type and five variants. High titres of anti-S antibodies and neutralising antibodies were observed, excepted for variant B. Read More

View Article and Full-Text PDF

COVID-19-associated Isolated Cortical Vein Thrombosis: Detection of SARS-CoV-2 in CSF.

Neurohospitalist 2022 Jul 4;12(3):520-523. Epub 2022 Mar 4.

Neurology Department, Haseki Research and Training Hospital, University of Health Science, Istanbul, Turkey.

COVID-19 has been associated with central nervous system manifestations; however, cerebral venous thrombosis is rarely reported. A 34-year-old woman was admitted to the hospital with headache and recurrent seizures; she was recently discharged after COVID-19 pneumonia. Cranial magnetic resonance imaging and magnetic resonance venography showed cortical vein thrombosis in the right frontal lobe. Read More

View Article and Full-Text PDF

Mixed invasive fungal infections among COVID-19 patients.

Curr Med Mycol 2021 Dec;7(4):19-27

Department of Pathology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.

Background And Purpose: The healthcare system in India collapsed during the second wave of the COVID-19 pandemic. A fungal epidemic was announced amid the pandemic with several cases of COVID-associated mucormycosis and pulmonary aspergillosis being reported. However, there is limited data regarding mixed fungal infections in COVID-19 patients. Read More

View Article and Full-Text PDF
December 2021

Repurposing Molnupiravir for COVID-19: The Mechanisms of Antiviral Activity.

Viruses 2022 Jun 20;14(6). Epub 2022 Jun 20.

School of Science, Monash University, Bandar Sunway, Subang Jaya 47500, Selangor Darul Ehsan, Malaysia.

Molnupiravir is a β-d-N4-hydroxycytidine-5'-isopropyl ester (NHC) compound that exerts antiviral activity against various RNA viruses such as influenza, SARS, and Ebola viruses. Thus, the repurposing of Molnupiravir has gained significant attention for combatting infection with SARS-CoV-2, the etiological agent of COVID-19. Recently, Molnupiravir was granted authorization for the treatment of mild-to-moderate COVID-19 in adults. Read More

View Article and Full-Text PDF

Resistance of SARS-CoV-2 Omicron BA.1 and BA.2 Variants to Vaccine-Elicited Sera and Therapeutic Monoclonal Antibodies.

Viruses 2022 Jun 18;14(6). Epub 2022 Jun 18.

Department of Microbiology, NYU Grossman School of Medicine, New York, NY 10016, USA.

The recent emergence of the Omicron BA.1 and BA.2 variants with heavily mutated spike proteins has posed a challenge to the effectiveness of current vaccines and to monoclonal antibody therapy for severe COVID-19. Read More

View Article and Full-Text PDF

Human Intramuscular Hyperimmune Gamma Globulin (hIHGG) Anti-SARS-CoV-2-Characteristics of Intermediates and Final Product.

Viruses 2022 Jun 17;14(6). Epub 2022 Jun 17.

Department of Virology, Institute of Hematology and Transfusion Medicine, Chocimska 5 Str., 00-957 Warsaw, Poland.

This study aims to characterize the intermediates, and the final product (FP) obtained during the production of human intramuscular hyperimmune gamma globulin anti-SARS-CoV-2 (hIHGG anti-SARS-CoV-2) and to determine its stability. : hIHGG anti-SARS-CoV-2 was fractionated from 270 convalescent plasma donations with the Cohn method. Prior to fractionation, the plasma was inactivated (Theraflex MB Plasma). Read More

View Article and Full-Text PDF

ORFeome Phage Display Reveals a Major Immunogenic Epitope on the S2 Subdomain of SARS-CoV-2 Spike Protein.

Viruses 2022 Jun 17;14(6). Epub 2022 Jun 17.

Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Spielmannstr 7, 38106 Braunschweig, Germany.

The development of antibody therapies against SARS-CoV-2 remains a challenging task during the ongoing COVID-19 pandemic. All approved therapeutic antibodies are directed against the receptor binding domain (RBD) of the spike, and therefore lose neutralization efficacy against emerging SARS-CoV-2 variants, which frequently mutate in the RBD region. Previously, phage display has been used to identify epitopes of antibody responses against several diseases. Read More

View Article and Full-Text PDF

Investigating COVID-19 Vaccine Impact on the Risk of Hospitalisation through the Analysis of National Surveillance Data Collected in Belgium.

Viruses 2022 Jun 16;14(6). Epub 2022 Jun 16.

Spatial Epidemiology Lab (SpELL), Université Libre de Bruxelles, 1050 Brussels, Belgium.

The national vaccination campaign against SARS-CoV-2 started in January 2021 in Belgium. In the present study, we aimed to use national hospitalisation surveillance data to investigate the recent evolution of vaccine impact on the risk of COVID-19 hospitalisation. We analysed aggregated data from 27,608 COVID-19 patients hospitalised between October 2021 and February 2022, stratified by age category and vaccination status. Read More

View Article and Full-Text PDF

Evaluation of Screening Program and Phylogenetic Analysis of SARS-CoV-2 Infections among Hospital Healthcare Workers in Liège, Belgium.

Viruses 2022 Jun 14;14(6). Epub 2022 Jun 14.

Department of Infectious Diseases and General Internal Medicine, University Hospital of Liège, 4000 Liege, Belgium.

Healthcare workers (HCWs) are known to be at higher risk of developing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections although whether these risks are equal across all occupational roles is uncertain. Identifying these risk factors and understand SARS-CoV-2 transmission pathways in healthcare settings are of high importance to achieve optimal protection measures. We aimed to investigate the implementation of a voluntary screening program for SARS-CoV-2 infections among hospital HCWs and to elucidate potential transmission pathways though phylogenetic analysis before the vaccination era. Read More

View Article and Full-Text PDF

Photodynamic Inactivation of SARS-CoV-2 Infectivity and Antiviral Treatment Effects In Vitro.

Viruses 2022 Jun 14;14(6). Epub 2022 Jun 14.

Department of Anaesthesiology and Intensive Care, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany.

Despite available vaccines, antibodies and antiviral agents, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic still continues to cause severe disease and death. Current treatment options are limited, and emerging new mutations are a challenge. Thus, novel treatments and measures for prevention of viral infections are urgently required. Read More

View Article and Full-Text PDF

Nationwide Harmonization Effort for Semi-Quantitative Reporting of SARS-CoV-2 PCR Test Results in Belgium.

Viruses 2022 Jun 14;14(6). Epub 2022 Jun 14.

Synlab Belgium, Synlab Laboratory Collard, 4020 Liège, Belgium.

From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the course of infection, the demand to report cycle threshold (Ct) values rapidly emerged. As Ct values can be affected by a number of factors, we considered that harmonization of semi-quantitative PCR results across laboratories would avoid potential divergent interpretations, particularly in the absence of clinical or serological information. Read More

View Article and Full-Text PDF

Infectious Aerosol Capture Mask as Environmental Control to Reduce Spread of Respiratory Viral Particles.

Viruses 2022 Jun 11;14(6). Epub 2022 Jun 11.

Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Negative pressure isolation of COVID-19 patients is critical to limiting the nosocomial transmission of SARS-CoV-2; however, airborne isolation rooms are limited. Alternatives to traditional isolation procedures are needed. The evaluation of an Infectious Aerosol Capture Mask (IACM) that is designed to augment the respiratory isolation of COVID-19 patients is described. Read More

View Article and Full-Text PDF

Targeted Virome Sequencing Enhances Unbiased Detection and Genome Assembly of Known and Emerging Viruses-The Example of SARS-CoV-2.

Viruses 2022 Jun 11;14(6). Epub 2022 Jun 11.

Laboratory of Medical Microbiology, Department of Microbiology, Hellenic Pasteur Institute, 11521 Athens, Greece.

Targeted virome enrichment and sequencing (VirCapSeq-VERT) utilizes a pool of oligos (baits) to enrich all known-up to 2015-vertebrate-infecting viruses, increasing their detection sensitivity. The hybridisation of the baits to the target sequences can be partial, thus enabling the detection and genomic reconstruction of novel pathogens with <40% genetic diversity compared to the strains used for the baits' design. In this study, we deploy this method in multiplexed mixes of viral extracts, and we assess its performance in the unbiased detection of DNA and RNA viruses after cDNA synthesis. Read More

View Article and Full-Text PDF

High and Sustained Ex Vivo Frequency but Altered Phenotype of SARS-CoV-2-Specific CD4 T-Cells in an Anti-CD20-Treated Patient with Prolonged COVID-19.

Viruses 2022 Jun 10;14(6). Epub 2022 Jun 10.

Infectious Diseases Unit I, Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

Here, we longitudinally assessed the ex vivo frequency and phenotype of SARS-CoV-2 membrane protein (aa145-164) epitope-specific CD4 T-cells of an anti-CD20-treated patient with prolonged viral positivity in direct comparison to an immunocompetent patient through an MHC class II DRB1*11:01 Tetramer analysis. We detected a high and stable SARS-CoV-2 membrane-specific CD4 T-cell response in both patients, with higher frequencies of virus-specific CD4 T-cells in the B-cell-depleted patient. However, we found an altered virus-specific CD4 T-cell memory phenotype in the B-cell-depleted patient that was skewed towards late differentiated memory T-cells, as well as reduced frequencies of SARS-CoV-2-specific CD4 T-cells with CD45RA CXCR5 PD-1 circulating T follicular helper cell (cT) phenotype. Read More

View Article and Full-Text PDF

Systemic Neutralizing Antibodies and Local Immune Responses Are Critical for the Control of SARS-CoV-2.

Viruses 2022 Jun 10;14(6). Epub 2022 Jun 10.

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.

Antibody measurements are primarily used to evaluate experimental and approved COVID-19 vaccines, which is unilateral considering our immune responses' complex nature. Previously, we showed that nanoparticle plasmid DNA adjuvant system, QAC, and MVA based vaccines were immunogenic against SARS-CoV-2. Here, we report on the protective efficacy of systemic humoral and mucosal cell-mediated immune responses in transgenic mice models against SARS-CoV-2 following nanoparticle immunization. Read More

View Article and Full-Text PDF

High Incidence of SARS-CoV-2 Variant of Concern Breakthrough Infections Despite Residual Humoral and Cellular Immunity Induced by BNT162b2 Vaccination in Healthcare Workers: A Long-Term Follow-Up Study in Belgium.

Viruses 2022 Jun 9;14(6). Epub 2022 Jun 9.

PharmAbs, the KU Leuven Antibody Center, KU Leuven, 3000 Leuven, Belgium.

To mitigate the massive COVID-19 burden caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), several vaccination campaigns were initiated. We performed a single-center observational trial to monitor the mid- (3 months) and long-term (10 months) adaptive immune response and to document breakthrough infections (BTI) in healthcare workers ( = 84) upon BNT162b2 vaccination in a real-world setting. Firstly, serology was determined through immunoassays. Read More

View Article and Full-Text PDF

SARS-CoV-2 Accessory Protein ORF8 Decreases Antibody-Dependent Cellular Cytotoxicity.

Viruses 2022 Jun 7;14(6). Epub 2022 Jun 7.

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Viruses use many different strategies to evade host immune responses. In the case of SARS-CoV-2, its Spike mutates rapidly to escape from neutralizing antibodies. In addition to this strategy, ORF8, a small accessory protein encoded by SARS-CoV-2, helps immune evasion by reducing the susceptibility of SARS-CoV-2-infected cells to the cytotoxic CD8+ T cell response. Read More

View Article and Full-Text PDF