2,348 results match your criteria Platelets[Journal]


Investigation of the contribution of an underlying platelet defect in women with unexplained heavy menstrual bleeding.

Platelets 2018 Dec 6:1-9. Epub 2018 Dec 6.

b Institute of Cardiovascular Sciences, College of Medical and Dental Sciences , University of Birmingham , Birmingham , UK.

Heavy menstrual bleeding (HMB) is often undiagnosed in women and can cause discomfort and distress. A haemostatic cause for excessive bleeding is often not routinely investigated and can lead to hysterectomy at an early age. A prospective cohort study was carried out to determine whether certain patients with unexplained HMB have an underlying platelet function defect (PFD). Read More

View Article
December 2018
2 Reads

Platelet reactivity in patients with chronic kidney disease undergoing percutaneous coronary intervention.

Platelets 2018 Dec 5:1-7. Epub 2018 Dec 5.

a Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases , The Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China.

This study aimed to evaluate the platelet reactivity in real-world patients with different chronic kidney disease (CKD) stages after percutaneous coronary intervention (PCI), and to examine whether high residual platelet reactivity (HRPR) is associated with higher incidence of adverse cardiovascular events in a 2-year follow up. A total of 10 724 consecutive patients receiving DAPT with aspirin and clopidogrel after PCI throughout 2013 were enrolled. We applied modified thromboelastography (mTEG) in 6745 patients. Read More

View Article
December 2018

Comparison of treatments for persistent/chronic immune thrombocytopenia: a systematic review and network meta-analysis.

Platelets 2018 Dec 3:1-11. Epub 2018 Dec 3.

a Department of Hematology and Oncology , Graduate School of Medicine, Kyoto University , Kyoto , Japan.

Recent studies have indicated that medical options without splenectomy, such as rituximab (RTX) or thrombopoietin receptor agonists (TPO-RAs), can be effective to treat persistent or chronic primary immune thrombocytopenia (ITP). However, it remains to be determined which of these strategies should be the first choice after the first-line treatment for newly diagnosed ITP. We performed a systematic review and network meta-analysis to establish a clinically meaningful hierarchy of the efficacy and safety of medical treatments for persistent or chronic ITP in adults. Read More

View Article
December 2018
2 Reads

Platelets - the second growth cycle.

Platelets 2018 Dec 4. Epub 2018 Dec 4.

c University of Birmingham , UK.

View Article
December 2018

Does plateletpheresis induce a hypercoagulable state? A global assessment of donor's hemostatic system by ROTEM.

Platelets 2018 Nov 29:1-5. Epub 2018 Nov 29.

c Department of Biostatistics , Eskişehir Osmangazi University Medical School , Eskisehir , Turkey.

Since there is still debate on the effects of plateletpheresis on coagulation system, we aimed to perform a global assessment of donor's hemostatic function undergoing plateletpheresis by rotation thromboelastometry (ROTEM) analysis and to clarify if plateletpheresis procedure induces a hypercoagulable state. Thirty male plateletpheresis donors were included in the study. Four blood samples were drawn at different time intervals: before the beginning of the apheresis procedure; immediately after the completion of the apheresis procedure; 24 h and 7 days after the apheresis procedure. Read More

View Article
November 2018

Clinical value of the quantitation of average daily platelet increase during the recovery period in childhood acute lymphoblastic leukaemia.

Platelets 2018 Nov 29:1-4. Epub 2018 Nov 29.

b Pediatric department of Hematology, Key Laboratory of Birth Defects and Related Diseases of Women and Children , Sichuan University , Chengdu , Sichuan , P.R. China.

The time to platelet recovery (TPR) is becoming a predicting factor during the treatment of childhood acute leukaemia. However, the initial pre-treatment platelet count (PPC) could interfere with TPR. Here, we integrated both TPR and PPC as the average daily platelet amount increase (Ap) to predict the prognosis in childhood B-ALL during the recovery period. Read More

View Article
November 2018

ABO antigen levels on platelets of normal and variant ABO blood group individuals.

Platelets 2018 Nov 26:1-7. Epub 2018 Nov 26.

a Blood Center of Zhejiang Province , Hangzhou , China.

Although differential expression of ABO antigens on platelets is related to recipients' platelet recovery and neonatal alloimmune thrombocytopenia in ABO-incompatible transfusion and pregnancy, the expression of ABO antigens on platelets in normal and variant ABO blood group individuals is rarely reported. Here, we analyzed the expression of ABO antigens on platelets in a cohort of 515 individuals with normal and variant ABO. The variants were also genotyped for ABO and/or FUT1 loci to distinguish inherited or acquired ABO variants. Read More

View Article
November 2018

Of platelets and aggregometers: personal reminiscences of Gus Born (1921-2018).

Authors:
Rod J Flower

Platelets 2018 Nov 26:1-7. Epub 2018 Nov 26.

a The William Harvey Research Institute , Queen Mary University of London , London , UK.

This paper recounts the author's personal reminiscences of the late Gustav Born and details some of his major influences on the field of platelet biology and mechanisms of hemostasis. In particular, it focuses on his development of the 'Born aggregometer' and the differences that are seen in the aggregation response to certain stimuli when aggregation is recorded using other techniques such as the impedance method. Read More

View Article
November 2018

CYCS gene variants associated with thrombocytopenia.

Platelets 2018 Nov 19:1-3. Epub 2018 Nov 19.

b Department of Pathology, Dunedin School of Medicine , University of Otago , Dunedin , New Zealand.

View Article
November 2018

Impact of immature platelet fraction on platelet reactivity during prasugrel maintenance treatment.

Platelets 2018 Nov 13:1-8. Epub 2018 Nov 13.

a Division of Cardiology , Azienda Ospedaliera-Universitaria "Maggiore della Carità", Eastern Piedmont University, Novara, Italy.

Residual high on-treatment platelet reactivity (HTPR) despite dual antiplatelet therapy (DAPT) has emerged as a predictor of major ischemic events in patients undergoing percutaneous coronary interventions (PCIs), especially after an acute cardiovascular event. However, its determinants are still poorly defined. Therefore, the aim of the present study was to evaluate the role of the percentage of reticulated platelets on HTPR in patients on DAPT with ASA (100-160 mg) and prasugrel (10 mg). Read More

View Article
November 2018
4 Reads

Chronic liver disease, thrombocytopenia and procedural bleeding risk; are novel thrombopoietin mimetics the solution?

Platelets 2018 Nov 13:1-3. Epub 2018 Nov 13.

a Division of Hematology and Medical Oncology , Oregon Health and Science University, Knight Cancer Institute , Portland , OR , USA.

Chronic liver disease (CLD) alters normal hemostatic and thrombotic systems via multiple mechanisms including reduced platelet function and number, leading to challenging peri-operative planning. Hepatic thrombopoietin (TPO) synthesis is reduced in CLD, leading to several recent randomized, placebo-controlled trials examining the utility of TPO-mimetics to increase platelet counts prior to surgery. While these trials do suggest that TPO-mimetics are efficacious at increasing platelet counts in patients with CLD and have led to several recent drug approvals in this space by the U. Read More

View Article
November 2018
15 Reads

Increased platelet reactivity and platelet-leukocyte aggregation after elective coronary bypass surgery.

Platelets 2018 Nov 13:1-7. Epub 2018 Nov 13.

b Department of Clinical Pharmacology, Karolinska University Hospital and Department of Medicine Solna , Karolinska Institutet , Stockholm , Sweden.

Inflammatory mechanisms are activated, and thrombotic complications occur during the initial months after coronary artery bypass grafting (CABG). Therefore, changes over time of platelet activation and platelet-leukocyte interactions after CABG are of interest. Whole-blood flow cytometry was performed before, and 4-6 days, one month, and three months after elective CABG in 54 men with stable coronary artery disease treated with acetylsalicylic acid (ASA). Read More

View Article
November 2018
4 Reads

Perioperative point of care platelet function testing and postoperative blood loss in high-risk cardiac surgery patients.

Platelets 2018 Nov 9:1-7. Epub 2018 Nov 9.

a Department of Anaesthesia, Intensive Care and Pain Medicine , St Antonius Hospital , Nieuwegein , The Netherlands.

Postoperative coagulopathic bleeding is common in cardiac surgery and associated with increased morbidity and mortality. Platelet function is affected by multiple factors, including patient and procedural characteristics. Point-of-care (POC) multiple electrode aggregometry (MEA) can rapidly detect and quantify platelet dysfunction and could contribute to optimal patient blood management. Read More

View Article
November 2018
5 Reads

Gustav Born: pioneer in imaging platelet and leukocyte biology.

Platelets 2018 Nov 9:1-5. Epub 2018 Nov 9.

a Sackler Institute of Pulmonary Pharmacology, Institute of Pharmaceutical Science , King's College London , London , UK.

Gustav Born achieved scientific fame for his application of light transmission aggregometry to the study of platelet function, but also led interdisciplinary research teams in pioneering quantitative in vivo imaging studies of platelet aggregation and leukocyte adhesion, and in conducting the first research into the biomechanical factors underlying atherosclerotic plaque rupture. Gus Born also communicated both current research findings and an integrated understanding of cardiovascular biology to a wide audience through acting as scientific advisor on several television productions. Using footage from two of these films, we discuss Gustav Born's scientific achievements and legacy. Read More

View Article
November 2018
5 Reads

Anagrelide is an anti-megakaryocytic and not an anti-platelet agent.

Platelets 2018 Nov 7:1-2. Epub 2018 Nov 7.

a IBR: Level 1, Institute of Cardiovascular Sciences, College of Medical and Dental Sciences , University of Birmingham , Birmingham , United Kingdom.

View Article
November 2018

SerpinB2 deficiency in mice reduces bleeding times via dysregulated platelet activation.

Platelets 2018 Nov 2:1-6. Epub 2018 Nov 2.

a QIMR Berghofer Medical Research Institute , Brisbane , Australia.

SerpinB2, also known as plasminogen activation inhibitor type 2 (PAI-2), is classically viewed as an inhibitor of fibrinolysis. However, we show herein a distinct, hitherto unrecognized role for SerpinB2 in hemostasis. Mice deficient in SerpinB2 expression and mice with an active site mutation in SerpinB2, both showed significant reductions in tail bleeding times. Read More

View Article
November 2018
1 Read

Severe obesity and bariatric surgery alter the platelet mRNA profile.

Platelets 2018 Nov 2:1-8. Epub 2018 Nov 2.

a Department of Medicine, Leon H. Charney Division of Cardiology and the Center for the Prevention of Cardiovascular Disease , New York University School of Medicine , New York , NY , USA.

Mechanisms explaining the relationship between obesity and cardiovascular disease (CVD) are needed. Despite growing recognition of the importance of the anucleate platelet transcriptome, low levels of RNA in platelets make assessment difficult. We sought to perform unbiased platelet RNA profiling in obesity by performing a prospective study of severe obesity and weight loss via bariatric surgery on platelet characteristics and mRNA profile in 26 pre-menopausal, non-diabetic women (31. Read More

View Article
November 2018
3 Reads

Gus Born: Scientific Reflections of Later Years, Inspiring the Next Generation of Blood Researchers.

Platelets 2018 Oct 30:1-3. Epub 2018 Oct 30.

a Systems Immunity Research Institute, School of Medicine, Cardiff University , Cardiff , Wales , UK.

I am delighted to have been be asked by Steve Watson to write a short piece for this series in Platelets, on my friendship with Prof Gus Born which began in 2011, when he came to Cardiff to present at our UK Platelets meeting. Gus was an insipiration, his story resonates today and it is an honour to have the opportunity to record a summary of our meetings here. Read More

View Article
October 2018

Gustav Born and life as "a series of ripples widening out from an original centre".

Authors:
Carlo Patrono

Platelets 2018 Oct 30:1-2. Epub 2018 Oct 30.

a Department of Pharmacology , Catholic University School of Medicine , Rome , Italy.

View Article
October 2018
3 Reads

Multifocal avascular necrosis in a patient with refractory immune thrombocytopenia and antiphospholipid antibodies; case report and review of literature.

Platelets 2018 Oct 29:1-8. Epub 2018 Oct 29.

a Rheumatology & Clinical Immunology Unit, Internal Medicine Department, Faculty of Medicine , Cairo University , Cairo , Egypt.

Avascular necrosis (AVN) is a devastating condition that is rarely reported in patients with immune thrombocytopenia (ITP). Treatment with steroids remains a major risk factor for developing AVN. However, the incidence of AVN in patients with ITP requiring corticosteroid therapy is much less than that observed with other clinical conditions requiring corticosteroids. Read More

View Article
October 2018
1 Read

Small GTPases in platelet membrane trafficking.

Platelets 2018 Oct 26:1-10. Epub 2018 Oct 26.

a From the School of Physiology, Pharmacology and Neuroscience, Biomedical Sciences Building , University of Bristol , Bristol , UK.

Our understanding of fundamental biological processes within platelets is continually evolving. A critical feature of platelet biology relates to the intricate uptake, packaging and release of bioactive cargo from storage vesicles, essential in mediating a range of classical (haemostasis/thrombosis) and non-classical (regeneration/inflammation/metastasis) roles platelets assume. Pivotal to the molecular control of these vesicle trafficking events are the small GTPases of the Ras superfamily, which function as spatially distinct, molecular switches controlling essential cellular processes. Read More

View Article
October 2018
4 Reads

Evaluation of the Total Thrombus-Formation System (T-TAS): application to human and mouse blood analysis.

Platelets 2018 Oct 26:1-8. Epub 2018 Oct 26.

a Institute of Inflammation and Ageing , University of Birmingham , Birmingham , UK.

The Total Thrombus-formation Analyser System (T-TAS) is a whole blood flow chamber system for the measurement of in vitro thrombus formation under variable shear stress conditions. Our current study sought to evaluate the potential utility of the T-TAS for the measurement of thrombus formation within human and mouse whole blood. T-TAS microchips (collagen, PL chip; collagen/tissue thromboplastin, AR chip) were used to analyze platelet (PL) or fibrin-rich thrombus formation, respectively. Read More

View Article
October 2018
5 Reads

An interview with Professor Gus Born.

Platelets 2018 Oct 26:1-5. Epub 2018 Oct 26.

b Faculté de pharmacie , Université de Montréal , Montréal , Québec , Canada.

This article is taken from an interview with Professor Gustav Victor Rudolf Born (known as Gus), and focuses on his personal reflections and his distinguished career. Professor Born's innovative research led to the development of a pioneering device, the aggregometer, which opened up the field of platelet research. In this article, Professor Born gives his modest insight into the early stages of his career and the impact Hiroshima had on his decision to work on thrombosis and hemostasis. Read More

View Article
October 2018

Platelet function after cardiac surgery and its association with severe postoperative bleeding: the PLATFORM study.

Platelets 2018 Oct 26:1-7. Epub 2018 Oct 26.

a Department of Cardiothoracic, Vascular Anaesthesia and Intensive Care , IRCCS Policlinico San Donato , Milan , Italy.

Platelet dysfunction after cardiac surgery is a determinant of postoperative bleeding. The existing guidelines suggest the use of desmopressin and/or platelet concentrate transfusions in case of platelet dysfunction in bleeding patients, but no cut-off values for platelet activity exist in the literature. The Platelet Function in the Operating Room (PLATFORM) study aims to identify the relationship between platelet function after cardiopulmonary bypass and severe bleeding, finding adequate predictive values of platelet function for severe bleeding. Read More

View Article
October 2018
3 Reads

Agreement between blood draw techniques for assessing platelet activation by flow cytometry.

Platelets 2018 Oct 26:1-5. Epub 2018 Oct 26.

a Respiratory Research Group, Hull York Medical School, Castle Hill Hospital , Cottingham , UK.

It is widely believed that assays of platelet activation are susceptible to preanalytical variables related to blood draw technique. We assessed platelet activation by whole blood flow cytometry and investigated the effects of: (1) drawing blood into vacuum tubes or manually aspirated syringes, and (2) discarding the first drawn blood sample (discard tube). Platelet P-selectin expression and platelet-monocyte complexes were measured by flow cytometry under both basal conditions and following stimulation with 0. Read More

View Article
October 2018
4 Reads

Influence of concentration and preparation of platelet rich fibrin on human bone marrow mononuclear cells (in vitro).

Platelets 2018 Oct 25:1-10. Epub 2018 Oct 25.

b Clinic for Maxillofacial and Plastic Surgery , FORM, Frankfurt Oral Regenerative Medicine, Johann Wolfgang Goethe University , Frankfurt Am Main , Germany.

Large bone defects have always been a big challenge. The use of bone marrow mononuclear cells (BMCs) combined with an osteoconductive scaffold has been proved a good alternative for the treatment of large bone defects. Another autologous source for tissue engineering is platelet rich fibrin (PRF). Read More

View Article
October 2018

MicroRNA function in megakaryocytes.

Platelets 2018 Oct 25:1-8. Epub 2018 Oct 25.

a Stem Cells and Haematological Disorders Laboratory, Department of Biochemistry , School of Life Sciences, University of Hyderabad , Hyderabad , TS , India.

Megakaryocytes (MKs), the largest cells in the bone marrow, are generated from hematopoietic stem cells (HSCs) in a sequential process called megakaryocytopoiesis in which HSCs undergo MK-progenitor (MP) commitment and maturation to terminally differentiated MK. Megakaryocytopoiesis is controlled by a complex network of bone marrow niche factors. Traditionally, the studies on megakaryocytopoiesis were focused on different cytokines, growth factors and transcription factors as the regulators of megakaryocytopoiesis. Read More

View Article
October 2018
4 Reads
2.630 Impact Factor

High-intensity intermittent exercise increases adenosine hydrolysis in platelets and lymphocytes and promotes platelet aggregation in futsal athletes.

Platelets 2018 Oct 22:1-8. Epub 2018 Oct 22.

d Academic Coordination, Medicine, Campus Chapecó , Federal University of Fronteira Sul , Chapecó , SC , Brazil.

Acute bouts of high-intensity intermittent exercise (HIIE) or sports are associated with changes in lymphocytes and platelet functions and we hypothesized that the purinergic system is involved with these alterations. We investigated the activity of ectonucleotidases in platelets and lymphocytes as well as the platelet aggregation of futsal players in response to an acute protocol of HIIE. Thus, 19 male semi-professional futsal players were submitted to 40 min of HIIE on a treadmill. Read More

View Article
October 2018
2 Reads

Methods papers in Platelets Journal.

Platelets 2018 Oct 22. Epub 2018 Oct 22.

a John Curtin School of Medical Research , The Australian National University , Canberra , ACT , Australia.

The utility of a methods section of a research paper is often tempered by the brevity demanded by manuscript word limitations. Whilst word limits help streamline a paper, a Methods section often bears the brunt of the editorial scalpel, resulting in only brief sketches of experimental protocols and consignment of methodology to online supplementary information files. To retain a place for important detailed methodology, and to encapsulate and highlight new and existing important techniques for platelet and megakaryocyte biology, the Platelets Journal Editorial board now accept Methods manuscripts. Read More

View Article
October 2018

Clinical importance of thrombocytopenia in patients with acute coronary syndromes: a systematic review and meta-analysis.

Platelets 2018 Oct 22:1-11. Epub 2018 Oct 22.

b Faculté de pharmacie , Research Center, Montreal Heart Institute , Montreal , Quebec , Canada.

Thrombocytopenia (TP) is common in hospitalized patients. In the context of acute coronary syndromes (ACS), TP has been linked to adverse clinical outcomes. We present a systematic review and meta-analysis of the evidence on the clinical importance of preexisting and in-hospital acquired TP in the context of ACS. Read More

View Article
October 2018
5 Reads

Aspirin intake in the morning is associated with suboptimal platelet inhibition, as measured by serum Thromboxane B during infarct-prone early-morning hours.

Platelets 2018 Oct 22:1-7. Epub 2018 Oct 22.

a Department of Internal Medicine , Amsterdam University Medical Center, VU University , Amsterdam , The Netherlands.

Aspirin is traditionally taken once daily in the morning and considered to be effective throughout the 24h interval. Cardiovascular events occur most frequently in the early morning, suggesting that these hours are critical in terms of adequate platelet inhibition. This study therefore assed platelet function in the early morning-8. Read More

View Article
October 2018
5 Reads

Blood collection, platelet isolation and measurement of platelet count and size in mice-a practical guide.

Platelets 2018 Oct 22:1-10. Epub 2018 Oct 22.

a Institute of Experimental Biomedicine I, University Hospital and Rudolf Virchow Center , University of Würzburg , Würzburg , Germany.

Inherited or acquired disorders of platelet production and function can result in thrombocytopenia and bleeding. Mouse models have proven useful for investigating the mechanisms that underlie these defects in humans. Precise methods for blood withdrawal, platelet isolation and measurement of platelet parameters are key for the generation of reproducible and conclusive data. Read More

View Article
October 2018
3 Reads

In celebration of Professor Gus Born's life, 29 July 1921 - 16 April 2018.

Platelets 2018 Oct 22. Epub 2018 Oct 22.

c Institute of Biomedical Research , University of Birmingham , Birmingham , UK.

View Article
October 2018

Association of thrombocytopenia with in-hospital outcome in patients with acute ST-segment elevated myocardial infarction.

Platelets 2018 Oct 22:1-10. Epub 2018 Oct 22.

a From Department of Cardiology , Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College , Beijing , China.

This study investigated the association of thrombocytopenia (TP) with in-hospital medication and outcome of patients with acute ST-segment elevated myocardial infarction (STEMI). A total of 16,678 consecutive patients with STEMI from multiple centers that participated in the China Acute Myocardial Infarction registry was included. In-hospital adverse event rates were compared between patients with TP and those with a normal platelet count. Read More

View Article
October 2018
4 Reads

High dose dexamethasone as an alternative rescue therapy for active bleeding in children with chronic ITP: clinical and immunological effects.

Platelets 2018 Oct 22:1-7. Epub 2018 Oct 22.

a Assiut Children Hospital, Faculty of Medicine , Assiut University , Assiut , Egypt.

High-dose dexamethasone (HD-DXM) is debated as a second-line therapy for chronic Immune thrombocytopenia (ITP) in children. The aim of this study is to evaluate the efficacy and safety of HD-DXM as an emergency therapy in uncontrolled bleeding in children with chronic ITP and to assess its immunological effect on dendritic cells (DCs) percentage and their co-stimulatory markers CD86 and CD83. Totally, 20 children previously diagnosed as chronic ITP were enrolled in this study and all admitted to hospital with uncontrolled bleeding. Read More

View Article
October 2018
1 Read

Sushi Domain Containing 2 (SUSD2) inhibits platelet activation and binding to high-grade serous ovarian carcinoma cells.

Platelets 2018 Oct 18:1-4. Epub 2018 Oct 18.

a Department of Biology , Augustana University , Sioux Falls , SD , USA.

Platelets play a central role in primary hemostasis affecting tumor survival and metastases. Tumors induce platelets to aggregate and bind to the cancer cells, resulting in protection from immune surveillance and often leading to thrombocytosis. In ovarian cancer (OvCa), one-third of patients present with thrombocytosis, a diagnosis that correlates with shorter survival. Read More

View Article
October 2018

A novel germline mutation in GP1BA gene N-terminal domain in monoallelic Bernard-Soulier syndrome.

Platelets 2018 Oct 17:1-7. Epub 2018 Oct 17.

a Department of Internal Medicine- Hematology and Oncology, University Hospital Brno and Faculty of Medicine , Masaryk University , Brno , Czech Republic.

Mutations in the GP1BA gene have been associated with platelet-type von Willebrand disease and Bernard-Soulier syndrome. Here, we report a novel GP1BA mutation in a family with autosomal dominant macrothrombocytopenia and mild bleeding. We performed analyses of seven family members. Read More

View Article
October 2018
4 Reads

Regulation of differentiation of MEG01 to megakaryocytes and platelet-like particles by Valproic acid through Notch3 mediated actin polymerization.

Platelets 2018 Oct 17:1-16. Epub 2018 Oct 17.

a National Centre for Cell Science, NCCS Complex , Savitribai Phule Pune University Campus , Pune , India.

Valproic acid (VPA) is one of the HDAC inhibitors used for the treatment of neurological disorders and hematological malignancies. Its role in self-renewal and proliferation of hematopoietic stem cells (HSCs) is well studied, but little is known about its involvement in regulating megakaryopoiesis and thrombopoiesis. In this study, we evaluated the role of VPA in megakaryopoiesis by using MEG-01, a megakaryoblast cell line. Read More

View Article
October 2018
2 Reads

Assessment of light transmission aggregometry on the routine coagulation analyzer Sysmex CS-2500 using CE-marked agonists from Hyphen Biomed.

Platelets 2018 Oct 12:1-3. Epub 2018 Oct 12.

a Laboratory of Hematology , University Hospital of Bordeaux , Pessac , France.

Light transmission aggregometry (LTA) is still considered as the "gold standard" for platelet function assessment but, as acompletely manual technology, it is labour intensive. This challenge can be overcome by performing platelet aggregometry in anautomated method on a routine coagulation analyzer. We aimed to compare and correlate results obtained from a traditional manual LTA solution realized in our Reference Center with an optimized automated system using CE-marked agonist reagents. Read More

View Article
October 2018
2 Reads

Multirefractory primary immune thrombocytopenia; targeting the decreased sialic acid content.

Platelets 2018 Oct 8:1-9. Epub 2018 Oct 8.

a Centro Regional de Hemodonacion. Servicio de Hematologia y Oncologia Medica , Hospital Universitario Morales Meseguer. IMIB-Arrixaca. Universidad de Murcia , Murcia , Spain.

Patients with multirefractory immune thrombocytopenia (ITP) have limited treatment options. Recent data suggest that specific anti-platelet antibodies may cause destruction of platelets by favoring platelet loss of sialic acid. In this multicenter study 35 patients with ITP, including 16 with multirefractory disease, were analyzed for antiplatelet-antibodies, thrombopoietin (TPO) levels, and platelet desialylation. Read More

View Article
October 2018
5 Reads

Flow cytometry for pediatric platelets.

Platelets 2018 Oct 4:1-10. Epub 2018 Oct 4.

a Cellular Hemostasis and Thrombosis Lab , National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Russian Ministry of Healthcare , Moscow , Russian Federation.

The ability of platelets to carry out their hemostatic function can be impaired in a wide range of inherited and acquired conditions: trauma, surgery, inflammation, pre-term birth, sepsis, hematological malignancies, solid tumors, chemotherapy, autoimmune disorders, and many others. Evaluation of this impairment is vitally important for research and clinical purposes. This problem is particularly pronounced in pediatric patients, where these conditions occur frequently, while blood volume and the choice of blood collection methods could be limited. Read More

View Article
October 2018
4 Reads

The effects of aging, diabetes mellitus, and antiplatelet drugs on growth factors and anti-aging proteins in platelet-rich plasma.

Platelets 2018 Sep 25:1-7. Epub 2018 Sep 25.

a Guangzhou School of Clinical Medicine , Southern Medical University (Guangzhou General Hospital of Guangzhou Military Region) , Guangzhou , Guangdong , China.

As the aged population continues to markedly increase worldwide, the incidences of diabetes mellitus (DM) and cardiovascular disease (CVD) are increasing. In this study, we investigated the effects of aging, DM, and antiplatelet drugs on growth factors and anti-aging proteins in platelet-rich plasma (PRP). The study participants were classified into the following four groups: Group A, healthy individuals aged ≤45 years; Group B, healthy individuals aged >45 years; Group C, DM patients aged >45 years; and Group D, CVD patients aged >45 years taking antiplatelet drugs. Read More

View Article
September 2018
2 Reads

A new aspect of in vitro antimicrobial leukocyte- and platelet-rich plasma activity based on flow cytometry assessment.

Platelets 2018 Sep 25:1-9. Epub 2018 Sep 25.

e Department of Orthopaedics , Medical University of Silesia, Trauma Center , Sosnowiec , Poland.

The current literature suggests that the antibacterial effect of leukocyte- and platelet-rich plasma (L-PRP) is directly related to platelet and leukocyte concentrations. The aim of this study was twofold: first, to evaluate the antimicrobial effect of L-PRP against selected bacterial strains in vitro, and second, to correlate this effect with leukocyte and platelet content in the final concentration. Blood was collected from 20 healthy males, and L-PRP, acellular plasma (AP), and autologous thrombin were consecutively prepared. Read More

View Article
September 2018

Cobimetinib and trametinib inhibit platelet MEK but do not cause platelet dysfunction.

Platelets 2018 Sep 25:1-11. Epub 2018 Sep 25.

a Institute for Cardiovascular and Metabolic Research , University of Reading, School of Biological Sciences , Reading , UK.

The MEK inhibitors cobimetinib and trametinib are used in combination with BRAF inhibitors to treat metastatic melanoma but increase rates of hemorrhage relative to BRAF inhibitors alone. Platelets express several members of the MAPK signalling cascade including MEK1 and MEK2 and ERK1 and ERK2 but their role in platelet function and haemostasis is ambiguous as previous reports have been contradictory. It is therefore unclear if MEK inhibitors might be causing platelet dysfunction and contributing to increased hemorrhage. Read More

View Article
September 2018

The platelet shape change: biophysical basis and physiological consequences.

Platelets 2018 Sep 25:1-6. Epub 2018 Sep 25.

a Laboratory of Optics and Dynamics of Biological Systems , Novosibirsk State University , Novosibirsk , Russia.

The well-known platelet shape change is the universal hallmark of activation. This review uncovers the biophysics underlying this rapid and dramatic transformation. We aim to give a broad vision of the interplay between different cytoskeletal subsystems, which is based on physical considerations and recent advances in mathematics and computational biology. Read More

View Article
September 2018

Effects of different doses of ticagrelor on platelet aggregation and endothelial function in diabetic patients with stable coronary artery disease.

Platelets 2018 Sep 25:1-10. Epub 2018 Sep 25.

a Cardiovascular Department , the First Affiliated Hospital of Harbin Medical University , Harbin , Heilongjiang , P.R. China.

We performed this study to observe the effects of different doses of ticagrelor and standard-dose clopidogrel on platelet reactivity and endothelial function in diabetic patients with stable coronary artery disease (CAD). Sixty type 2 diabetic patients were assigned to one-quarter standard-dose ticagrelor, half standard-dose ticagrelor, standard-dose ticagrelor and standard-dose clopidogrel groups. Light transmission aggregometry (LTA) and VerifyNow assay were used to measure platelet function. Read More

View Article
September 2018
8 Reads

Assays for phenotypic and functional characterization of cryopreserved platelets.

Platelets 2018 Sep 25:1-8. Epub 2018 Sep 25.

a Research and Development , Australian Red Cross Blood Service , Sydney , NSW , Australia.

Platelet concentrates for transfusion have a limited shelf-life, and cryopreservation offers a means of extending platelet shelf-life for at least 2 years. Cryopreservation, however, has some disadvantages, as platelets can be damaged during the freezing and thawing process. Consequently the phenotype of cryopreserved platelets is very different to that of freshly collected, liquid stored platelets. Read More

View Article
September 2018
10 Reads

Dynamic platelet function is markedly different in patients with cancer compared to healthy donors.

Platelets 2018 Sep 25:1-6. Epub 2018 Sep 25.

a Molecular and Cellular Therapeutics , The Royal College of Surgeons in Ireland , Dublin , Ireland.

Despite a fivefold increased risk of thromboembolism in patients with cancer, the mechanism of arterial thromboembolism is poorly understood. To address this, we investigated platelet function in cancer patients and healthy controls using an assay that mimics the arterial vasculature. Blood samples from cancer patients (n = 36) and healthy controls (n = 22) were perfused through custom-made parallel-plate flow chambers coated with von Willebrand factor (VWF) under arterial shear (1,500 s). Read More

View Article
September 2018

Pharmacodynamic safety of clopidogrel monotherapy in patients under oral anticoagulation with a vitamin K antagonist undergoing coronary stent implantation.

Platelets 2018 Sep 13:1-6. Epub 2018 Sep 13.

a Department of Cardiology and Angiology II , University Heart Center Freiburg Bad Krozingen , Bad Krozingen , Germany.

Current guidelines recommend as treatment option in patients on oral anticoagulation (OAC) undergoing percutaneous coronary intervention (PCI) an antiplatelet monotherapy with clopidogrel if there is an increased risk for bleeding. However, retrospective data suggested a potential interaction of clopidogrel and the vitamin K antagonist (VKA) phenprocoumon leading to a diminished antiplatelet effect. This would increase the ischemic risk of patients treated with this combination. Read More

View Article
September 2018
5 Reads

Platelets and cancer: pathology and drug targets.

Platelets 2018 Sep 11:1-2. Epub 2018 Sep 11.

a Clinical and Laboratory Medicine, Faculty of Medicine , University of Yamanashi , Chuo , Yamanashi , Japan.

View Article
September 2018
1 Read