1,702 results match your criteria Plant biotechnology journal[Journal]


Establishing CRISPR/Cas13a immune system conferring RNA virus resistance in both dicot and monocot plants.

Plant Biotechnol J 2019 Feb 20. Epub 2019 Feb 20.

Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.

Besides its powerful capability for genome editing, the clustered, regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) systems, has been exploited to combat virus infection in eukaryotic organisms (Zaidi et al., 2016). By harnessing CRISPR/Cas system, its compelling inhibiting activities against DNA viruses (Ali et al. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13095DOI Listing
February 2019

Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses.

Plant Biotechnol J 2019 Feb 19. Epub 2019 Feb 19.

GAFL, INRA, Montfavet, France.

In many crop species, natural variation in eIF4E proteins confers resistance to potyviruses. Gene editing offers new opportunities to transfer genetic resistance to crops that seem to lack natural eIF4E alleles. However, because eIF4E are physiologically important proteins, any introduced modification for virus resistance must not bring adverse phenotype effects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13096DOI Listing
February 2019

A single nucleotide mutation in GID1c disrupts its interaction with DELLA1 and causes a GA-insensitive dwarf phenotype in peach.

Plant Biotechnol J 2019 Feb 18. Epub 2019 Feb 18.

College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.

Plant stature is one important factor that affects the productivity of peach orchards. However, little is known about the molecular mechanism(s) underlying the dwarf phenotype of peach tree. Here, we report a dwarfing mechanism in the peach cv. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13094DOI Listing
February 2019

Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice.

Plant Biotechnol J 2019 Feb 16. Epub 2019 Feb 16.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081, China.

Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)-like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defense responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35), and cloning of the causal gene by TAIL-PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H O , up-regulated expression of defense-related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13093DOI Listing
February 2019
1 Read

Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilising inhibitor SlCYS8 in Nicotiana benthamiana.

Plant Biotechnol J 2019 Feb 11. Epub 2019 Feb 11.

Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, Oxford, United Kingdom.

Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed Activity-based Protein Profiling (ABPP) to identify proteases that are inhibited by SlCYS8 in agroinfiltrated Nicotiana benthamiana. We discovered that SlCYS8 selectively suppresses papain-like cysteine protease (PLCP) activity in both apoplastic fluids and total leaf extracts, while not affecting vacuolar processing enzyme and serine hydrolase activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13092DOI Listing
February 2019

The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa).

Plant Biotechnol J 2019 Feb 7. Epub 2019 Feb 7.

Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.

Multi-functional microRNAs (miRNAs) are emerging as key modulators of plant-pathogen interactions. Although the involvement of some miRNAs in plant-insect interactions has been revealed, the underlying mechanisms are still elusive. The brown planthopper (BPH) is the most notorious rice (Oryza sativa)-specific insect that causes severe yield losses each year and requires urgent biological control. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13091DOI Listing
February 2019

A Novel Anti-HIV-1 Bispecific bNAb-Lectin Fusion Protein Engineered in a Plant-based Transient Expression System.

Plant Biotechnol J 2019 Feb 6. Epub 2019 Feb 6.

James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.

The discovery of broadly neutralizing antibodies (bNAbs) has been a major step towards better prophylactic and therapeutic agents against human immunodeficiency virus type 1 (HIV-1). However, effective therapy will likely require a combination of anti-HIV agents to avoid viral evasion. One possible solution to this problem is the creation of bispecific molecules that can concurrently target two vulnerable sites providing synergistic inhibitory effects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13090DOI Listing
February 2019
2 Reads

High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.

Plant Biotechnol J 2019 Feb 1. Epub 2019 Feb 1.

Corteva AgriscienceTM, Agricultural Division of Dow DuPont, 8305 NW 62nd Avenue, Johnston, IA, 50131.

An efficient Agrobacterium-mediated site-specific integration (SSI) technology using the FLP/FRT system in elite maize inbred lines is described. The system allows precise integration of a single copy of a donor DNA flanked by heterologous FRT sites into a predefined recombinant target line (RTL) containing the corresponding heterologous FRT sites. A promoter-trap system consisting of a pre-integrated promoter followed by an FRT site enables efficient selection of events. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13089DOI Listing
February 2019

Development of an Agrobacterium-delivered CRISPR/Cas9 system for wheat genome editing.

Plant Biotechnol J 2019 Feb 1. Epub 2019 Feb 1.

Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA.

CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA delivery methods. In wheat, the biolistics based transformation is the most used method for delivery of the CRISPR/Cas9 complex. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13088
Publisher Site
http://dx.doi.org/10.1111/pbi.13088DOI Listing
February 2019
4 Reads

Haplotype analysis of key genes governing grain yield and quality traits across 3K RG panel reveals scope for the development of tailor-made rice with enhanced genetic gains.

Plant Biotechnol J 2019 Jan 30. Epub 2019 Jan 30.

International Rice Research Institute, South-Asia Hub, ICRISAT Campus, Hyderabad, India.

Though several genes governing various major traits have been reported in rice, their superior haplotype combinations for developing ideal variety remains elusive. In this study, haplotype analysis of 120 previously functionally characterized genes, influencing grain yield (87 genes) and grain quality (33 genes) revealed significant variations in the 3K rice genome (RG) panel. For selected genes, meta-expression analysis using already available datasets along with co-expression network provided insights at systems level. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13087DOI Listing
January 2019
1 Read

Development of an integrated 200K SNP genotyping array and application for genetic mapping, genome assembly improvement and genome wide association studies in pear (Pyrus).

Plant Biotechnol J 2019 Jan 28. Epub 2019 Jan 28.

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

Pear (Pyrus; 2n = 34), the third most important temperate fruit crop, has great nutritional and economic value. Despite the availability of many genomic resources in pear, it is challenging to genotype novel germplasm resources and breeding progeny in a timely and cost-effective manner. Genotyping arrays can provide fast, efficient and high-throughput genetic characterization of diverse germplasm, genetic mapping and breeding populations. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13085
Publisher Site
http://dx.doi.org/10.1111/pbi.13085DOI Listing
January 2019
13 Reads

Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode.

Plant Biotechnol J 2019 Jan 28. Epub 2019 Jan 28.

Division of Plant Sciences, University of Missouri, Columbia, MO, USA.

Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13086DOI Listing
January 2019

FON4 prevents the multi-floret spikelet in rice.

Plant Biotechnol J 2019 Jan 24. Epub 2019 Jan 24.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13083DOI Listing
January 2019
1 Read

Harnessed viruses in the age of metagenomics and synthetic biology: An update on infectious clone assembly and biotechnologies of plant viruses.

Plant Biotechnol J 2019 Jan 24. Epub 2019 Jan 24.

Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), 46022, Valencia, Spain.

Recent metagenomic studies have provided an unprecedented wealth of data, which is revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses as active players in the phytobiome, and surveys have uncovered their positive roles in environmental stress tolerance of plants. Viral infectious clones are key tools for functional characterization of known and newly identified viruses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13084DOI Listing
January 2019
1 Read

Resistance against Sclerotinia sclerotiorum in soybean involves a reprogramming of the phenylpropanoid pathway and up-regulation of antifungal activity targeting ergosterol biosynthesis.

Plant Biotechnol J 2019 Jan 22. Epub 2019 Jan 22.

Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, USA.

Sclerotinia sclerotiorum, a predominately necrotrophic fungal pathogen with a broad host range, causes a significant yield-limiting disease of soybean called Sclerotinia stem rot. Resistance mechanisms against this pathogen in soybean are poorly understood, thus hindering the commercial deployment of resistant varieties. We used a multiomic approach utilizing RNA-sequencing, gas chromatography-mass spectrometry-based metabolomics and chemical genomics in yeast to decipher the molecular mechanisms governing resistance to S. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13082DOI Listing
January 2019
3 Reads

Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering.

Plant Biotechnol J 2019 Jan 22. Epub 2019 Jan 22.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany.

Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid and versatile expression system which involves the infusion of Agrobacterium tumefaciens into three-dimensional, porous plant cell aggregates deprived of cultivation medium, which we have termed plant cell packs (PCPs). This approach is compatible with different plant species such as Nicotiana tabacum BY2, Nicotiana benthamiana or Daucus carota and 10-times more effective than transient expression in liquid plant cell culture. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13081DOI Listing
January 2019
4 Reads

Natural alleles of GLA for grain length and awn development were differently domesticated in rice subspecies japonica and indica.

Plant Biotechnol J 2019 Jan 21. Epub 2019 Jan 21.

Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China.

Rice (Oryza sativa L.) cultivars harbor morphological and physiological traits different from those of wild rice (O. rufipogon Griff. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13080DOI Listing
January 2019
2 Reads

PBJ is ranked higher, publishes more original articles and offers free global access.

Authors:
Henry Daniell

Plant Biotechnol J 2019 Jan;17(1):3-4

Director of Translational Research, University of Pennsylvania, Philadelphia, PA, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330532PMC
January 2019
1 Read

Glycolate from microalgae: an efficient carbon source for biotechnological applications.

Plant Biotechnol J 2019 Jan 13. Epub 2019 Jan 13.

Department of Plant Physiology, Institute of Biology, University of Leipzig, Leipzig, Germany.

Glycolate is produced in autotrophic cells under high temperatures and C -limitation via oxygenation of ribulose-1,5-bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C cycle by consuming reductants, ATP and CO emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13078
Publisher Site
http://dx.doi.org/10.1111/pbi.13078DOI Listing
January 2019
5 Reads

Creating highly efficient resistance against wheat dwarf virus in barley by employing CRISPR/Cas9 system.

Plant Biotechnol J 2019 Jan 11. Epub 2019 Jan 11.

National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, Gödöllő, Hungary.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13077
Publisher Site
http://dx.doi.org/10.1111/pbi.13077DOI Listing
January 2019
17 Reads

Plant ISOform sequencing database (PISO): a comprehensive repertory of full-length transcripts in plants.

Plant Biotechnol J 2019 Jan 11. Epub 2019 Jan 11.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13076DOI Listing
January 2019
1 Read

The basic helix-loop-helix transcription factor, OsPIL15, regulates grain size via directly targeting a purine permease gene OsPUP7 in rice.

Plant Biotechnol J 2019 Jan 9. Epub 2019 Jan 9.

Henan Key Laboratory of Rice Biology, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.

As members of the basic helix-loop-helix transcription factor families, phytochrome-interacting factors regulate an array of developmental responses ranging from seed germination to plant growth. However, little is known about their roles in modulating grain development. Here, we firstly analyzed the expression pattern of rice OsPIL genes in grains and found that OsPIL15 may play an important role in grain development. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13075DOI Listing
January 2019
2 Reads

Metabolic engineering of bread wheat improves grain iron concentration and bioavailability.

Plant Biotechnol J 2019 Jan 8. Epub 2019 Jan 8.

School of BioSciences, The University of Melbourne, Melbourne, VIC, Australia.

Bread wheat (Triticum aestivum L.) is cultivated on more land than any other crop and produces a fifth of the calories consumed by humans. Wheat endosperm is rich in starch yet contains low concentrations of dietary iron (Fe) and zinc (Zn). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13074DOI Listing
January 2019
2 Reads

Overexpression of Loose Plant Architecture 1 increases planting density and resistance to sheath blight disease via activation of PIN-FORMED 1a in rice.

Plant Biotechnol J 2019 Jan 8. Epub 2019 Jan 8.

College of Plant Protection, Shenyang Agricultural University, Shenyang, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13072DOI Listing
January 2019
1 Read

IDD16 negatively regulates stomatal initiation via trans-repression of SPCH in Arabidopsis.

Plant Biotechnol J 2019 Jan 8. Epub 2019 Jan 8.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

In Arabidopsis, the initiation and proliferation of stomatal lineage cells is controlled by SPEECHLESS (SPCH). Phosphorylation of SPCH at the post-translational level has been reported to regulate stomatal development. Here we report that IDD16 acts as a negative regulator for stomatal initiation by directly regulating SPCH transcription. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13070DOI Listing
January 2019
1 Read

The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over-ripening properties.

Plant Biotechnol J 2019 Jan 8. Epub 2019 Jan 8.

School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.

Tomato fruit are an important nutritional component of the human diet and offer potential to act as a cell factory for speciality chemicals, which are often produced by chemical synthesis. In the present study our goal was to produce competitive levels of the high value ketocarotenoid, astaxanthin, in tomato fruit. The initial stage in this process was achieved by expressing the 4, 4' carotenoid oxygenase (crtW) and 3, 3' hydroxylase (crtZ) from marine bacteria in tomato under constitutive control. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13073
Publisher Site
http://dx.doi.org/10.1111/pbi.13073DOI Listing
January 2019
2 Reads

A strong early-acting wound-inducible promoter, RbPCD1pro, activates cryIAc expression within minutes of wounding to impart efficient protection against insects.

Plant Biotechnol J 2019 Jan 8. Epub 2019 Jan 8.

Plant Gene Expression Lab, and 4Genomics and Molecular Biology Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India.

The expression of insecticidal proteins under constitutive promoters in transgenic plants is fraught with problems like developmental abnormalities, yield drag, expression in unwanted tissues and seasonal changes in expression. RbPCD1pro, a rapid, early-acting wound-inducible promoter from rose that is activated within 5 minutes of wounding, was isolated and characterized. Wounding increased transcript levels up to 150 and 500 folds within 5 and 20 minutes coupled with high translation as seen by histochemical GUS enzyme activity within 5-20 minutes. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13071
Publisher Site
http://dx.doi.org/10.1111/pbi.13071DOI Listing
January 2019
7 Reads
5.752 Impact Factor

OsACL-A2 negatively regulates cell death and disease resistance in rice.

Plant Biotechnol J 2018 Dec 24. Epub 2018 Dec 24.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang, China.

ATP-citrate lyases (ACL) play critical roles in tumour cell propagation, foetal development and growth, and histone acetylation in human and animals. Here, we report a novel function of ACL in cell death-mediated pathogen defence responses in rice. Using ethyl methanesulphonate (EMS) mutagenesis and map-based cloning, we identified an Oryza sativa ACL-A2 mutant allele, termed spotted leaf 30-1 (spl30-1), in which an A-to-T transversion converts an Asn at position 343 to a Tyr (N343Y), causing a recessive mutation that led to a lesion mimic phenotype. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13058
Publisher Site
http://dx.doi.org/10.1111/pbi.13058DOI Listing
December 2018
18 Reads

Silencing an essential gene involved in infestation and digestion in grain aphid through plant-mediated RNA interference generates aphid-resistant wheat plants.

Plant Biotechnol J 2018 Dec 24. Epub 2018 Dec 24.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13067DOI Listing
December 2018
1 Read

Enhancing carotenoid biosynthesis in rice endosperm by metabolic engineering.

Plant Biotechnol J 2018 Dec 24. Epub 2018 Dec 24.

Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13059DOI Listing
December 2018
8 Reads

Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing.

Plant Biotechnol J 2018 Dec 24. Epub 2018 Dec 24.

Department of Biotechnology, Center for Informational Biology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, China.

CRISPR-Cas9 and Cas12a are two powerful genome editing systems. Expression of CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas protein is expressed by a Pol II promoter and a guide RNA is expressed by a species-specific Pol III promoter such as U6 or U3. To achieve coordinated expression and compact vector packaging, it is desirable to express both CRISPR components under a single Pol II promoter. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13068DOI Listing
December 2018
3 Reads

Phytochrome A signal transduction 1 and CONSTANS-LIKE 13 coordinately orchestrate shoot branching and flowering in leafy Brassica juncea.

Plant Biotechnol J 2018 Dec 22. Epub 2018 Dec 22.

Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Sciences, Zhejiang University, Hangzhou, China.

Branching is a major determinant of crop yield, and enables vigorous shoot growth and the production of a dense canopy. Phytochrome A signal transduction 1 (PAT1) positively regulates phytochrome A signal transduction in response to light, but its effects on branching remain unknown. In this study, we mapped PAT1, and revealed a previously unknown role related to branching and flowering in leafy Brassica juncea. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13057
Publisher Site
http://dx.doi.org/10.1111/pbi.13057DOI Listing
December 2018
12 Reads

Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.

Plant Biotechnol J 2018 Dec 22. Epub 2018 Dec 22.

NIBIO - Norwegian Institute of Bioeconomy Research, Division of Biotechnology and Plant Health, Ås, Norway.

Dengue fever is a mosquito (Aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. There are four serotypes of the dengue virus (DENV 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. To date, the first vaccine, Dengvaxia (CYD-TDV), is available after many decades' efforts, but only has moderate efficacy. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13065
Publisher Site
http://dx.doi.org/10.1111/pbi.13065DOI Listing
December 2018
8 Reads

Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata.

Plant Biotechnol J 2018 Dec 22. Epub 2018 Dec 22.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Tetraploids have been reported to exhibit increased stress tolerance, but the underlying molecular and physiological mechanisms remain poorly understood. In this study, autotetraploid plants were identified by screening natural seedlings of trifoliate orange (Poncirus trifoliata). The tetraploids exhibited different morphology and displayed significantly enhanced drought and dehydration tolerance in comparison with the diploid progenitor. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13064
Publisher Site
http://dx.doi.org/10.1111/pbi.13064DOI Listing
December 2018
12 Reads

Isoform sequencing provides insight into natural genetic diversity in maize.

Plant Biotechnol J 2018 Dec 22. Epub 2018 Dec 22.

Department of Plant Sciences, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China.

W64A, as a member of non-stiff stalk maize, has been used to develop current corn in plant breeding, and serving as one of broadest parent line for the commercial hybrid seed production (Huffman, 1984). The inbred had the characteristics of early flowering, average plant and ear height at its maturity, very strong roots and good stalks (Runge, 2004). In addition, W64A serves as an invaluable germplasm to study gene functions especially in the field of corn nutrition and endosperm texture given its nearly complete vitreousness and hardness (Figure 1a). Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13063
Publisher Site
http://dx.doi.org/10.1111/pbi.13063DOI Listing
December 2018
2 Reads
5.752 Impact Factor

Cold chain and virus-free oral polio booster vaccine made in lettuce chloroplasts confers protection against all three poliovirus serotypes.

Plant Biotechnol J 2018 Dec 21. Epub 2018 Dec 21.

Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

To prevent vaccine-associated paralytic poliomyelitis, WHO recommended withdrawal of Oral Polio Vaccine (Serotype-2) and a single dose of Inactivated Poliovirus Vaccine (IPV). IPV however is expensive, requires cold chain, injections and offers limited intestinal mucosal immunity, essential to prevent polio reinfection in countries with open sewer system. To date, there is no virus-free and cold chain-free polio vaccine capable of inducing robust mucosal immunity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13060DOI Listing
December 2018
1 Read

A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat.

Plant Biotechnol J 2018 Dec 21. Epub 2018 Dec 21.

Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.

The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with an international elite durum wheat variety (Asassa). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13062DOI Listing
December 2018
1 Read

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed.

Plant Biotechnol J 2018 Dec 21. Epub 2018 Dec 21.

Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.

Soybean (Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13061DOI Listing
December 2018
2 Reads

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process.

Plant Biotechnol J 2018 Dec 21. Epub 2018 Dec 21.

Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Ethylene-responsive factors (ERFs) have been revealed to play essential roles in a variety of physiological and biological processes in higher plants. However, functions and regulatory pathways of most ERFs in cold stress remain largely unclear. Here, we identified PtrERF109 of trifoliate orange (Poncirus trifoliata (L. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13056
Publisher Site
http://dx.doi.org/10.1111/pbi.13056DOI Listing
December 2018
18 Reads

Overexpression of SrUGT76G1 in Stevia alters major steviol glycosides composition towards improved quality.

Plant Biotechnol J 2018 Dec 19. Epub 2018 Dec 19.

Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.

Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its sweeter and more pleasant taste compared to stevioside. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/pbi.13035
Publisher Site
http://dx.doi.org/10.1111/pbi.13035DOI Listing
December 2018
8 Reads

Allantoin accumulation through overexpression of ureide permease1 improves rice growth under limited nitrogen conditions.

Plant Biotechnol J 2018 Dec 19. Epub 2018 Dec 19.

Graduate School of International Agricultural Technology and Crop Biotechnology, Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.

In legumes, nitrogen (N) can be stored as ureide allantoin and transported by ureide permease (UPS) from nodules to leaves where it is catabolized to release ammonium and assimilation to amino acids. In non-leguminous plants especially rice, information on its roles in N metabolism is scarce. Here, we show that OsUPS1 is localized in plasma membranes and are highly expressed in vascular tissues of rice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13054DOI Listing
December 2018
2 Reads

Development of Beet necrotic yellow vein virus-based vectors for multiple-gene expression and guide RNA delivery in plant genome editing.

Plant Biotechnol J 2018 Dec 19. Epub 2018 Dec 19.

State Key Laboratory for Agro-biotechnology and Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.

Many plant viruses with monopartite or bipartite genomes have been developed as efficient expression vectors of foreign recombinant proteins. Nonetheless, due to lack of multiple insertion sites in these plant viruses, it is still a big challenge to simultaneously express multiple foreign proteins in single cells. The genome of Beet necrotic yellow vein virus (BNYVV) offers an attractive system for expression of multiple foreign proteins owning to a multipartite genome composed of five positive-stranded RNAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13055DOI Listing
December 2018
1 Read
5.752 Impact Factor

NF-YB1-YC12-bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.).

Plant Biotechnol J 2018 Dec 15. Epub 2018 Dec 15.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, China.

Identification of seed development regulatory genes is the key for the genetic improvement in rice grain quality. NF-Ys are the important transcription factors, but their roles in rice grain quality control and the underlying molecular mechanism remain largely unknown. Here, we report the functional characterization a rice NF-Y heterotrimer complex NF-YB1-YC12-bHLH144, which is formed by the binding of NF-YB1 to NF-YC12 and then bHLH144 in a sequential order. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13048DOI Listing
December 2018
3 Reads
5.752 Impact Factor

xCas9 expands the scope of genome editing with reduced efficiency in rice.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13053
Publisher Site
http://dx.doi.org/10.1111/pbi.13053DOI Listing
December 2018
3 Reads

Elucidating the genetic basis of biomass accumulation and radiation use efficiency in spring wheat and its role in yield potential.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

Global Wheat Program, International Maize and Wheat Improvement Centre (CIMMYT), Texcoco, Mexico.

One of the major challenges for plant scientists is increasing wheat (Triticum aestivum) yield potential (YP). A significant bottleneck for increasing YP is achieving increased biomass through optimization of radiation use efficiency (RUE) along the crop cycle. Exotic material such as landraces and synthetic wheat has been incorporated into breeding programmes in an attempt to alleviate this; however, their contribution to YP is still unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13052DOI Listing
December 2018
2 Reads

Integrating a genome-wide association study with a large-scale transcriptome analysis to predict genetic regions influencing the glycaemic index and texture in rice.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

International Rice Research Institute, Los Baños, Philippines.

Reliably generating rice varieties with low glycaemic index (GI) is an important nutritional intervention given the high rates of Type II diabetes incidences in Asia where rice is staple diet. We integrated a genome-wide association study (GWAS) with a transcriptome-wide association study (TWAS) to determine the genetic basis of the GI in rice. GWAS utilized 305 re-sequenced diverse indica panel comprising ~2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13051DOI Listing
December 2018
2 Reads
5.752 Impact Factor

Discovery of genomic regions and candidate genes controlling shelling percentage using QTL-seq approach in cultivated peanut (Arachis hypogaea L.).

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, China.

Cultivated peanut (Arachis hypogaea L.) is an important grain legume providing high-quality cooking oil, rich proteins and other nutrients. Shelling percentage (SP) is the 2nd most important agronomic trait after pod yield and this trait significantly affects the economic value of peanut in the market. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13050
Publisher Site
http://dx.doi.org/10.1111/pbi.13050DOI Listing
December 2018
3 Reads

Impact of differential DNA methylation on transgene expression in cotton (Gossypium hirsutum L.) events generated by targeted sequence insertion.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

BASF Agricultural Solutions Belgium NV, Gent, Belgium.

Targeted Genome Optimization (TGO) using site-specific nucleases to introduce a DNA double-strand break (DSB) at a specific target locus has broadened the options available to breeders for generation and combination of multiple traits. The use of targeted DNA cleavage in combination with homologous recombination (HR)-mediated repair, enabled the precise targeted insertion of additional trait genes (2mepsps, hppd, axmi115) at a pre-existing transgenic locus in cotton. Here we describe the expression and epigenome analyses of cotton Targeted Sequence Insertion (TSI) events over generations. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13049
Publisher Site
http://dx.doi.org/10.1111/pbi.13049DOI Listing
December 2018
2 Reads

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO.

Plant Biotechnol J 2018 Dec 7. Epub 2018 Dec 7.

Department of Plant and Environmental Sciences, Faculty of Science, Copenhagen University, Frederiksberg, Denmark.

Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13046DOI Listing
December 2018
3 Reads