1,740 results match your criteria Plant biotechnology journal[Journal]


Genome-wide association analyses reveal the genetic basis of combining ability in rice.

Plant Biotechnol J 2019 Apr 20. Epub 2019 Apr 20.

National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.

Combining ability is a measure for selecting elite parents and predicting hybrid performance in plant breeding. However, the genetic basis of combining ability remains unclear and a global view of combining ability from diverse mating designs is lacking. We developed a North Carolina II (NCII) population of 96 O. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13134
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http://dx.doi.org/10.1111/pbi.13134DOI Listing
April 2019
5 Reads

Genome sequencing and CRISPR/Cas9 gene editing of an early flowering mini citrus (Fortunella hindsii).

Plant Biotechnol J 2019 Apr 20. Epub 2019 Apr 20.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, P.R. China.

Hongkong kumquat (Fortunella hindsii) is a wild citrus species characterized by dwarf plant height and early flowering. Here, we identified the monoembryonic F. hindsii (designated as "Mini-Citrus") for the first time and constructed its selfing lines. Read More

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http://dx.doi.org/10.1111/pbi.13132DOI Listing

Identification of anthocyanin biosynthesis genes in rice pericarp using PCAMP.

Plant Biotechnol J 2019 Apr 20. Epub 2019 Apr 20.

Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.

Anthocyanins are a kind of biologically active flavonoids, which have strong anti-oxidation and anti-mutation functions as phytonutrients and have important effects on human health. The anthocyanin metabolic pathway has been extensively studied in Arabidopsis thaliana, Petunia hybrida and Zea mays etc., which involves many structural genes and regulatory genes. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13133
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http://dx.doi.org/10.1111/pbi.13133DOI Listing
April 2019
5 Reads

CRISPR/Cas9-mediated functional recovery of the recessive rc allele to develop red rice.

Plant Biotechnol J 2019 Apr 19. Epub 2019 Apr 19.

Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China.

Red rice contains high levels of proanthocyanidins and anthocyanins, which have been recognized as health-promoting nutrients. The red colouration of rice grains is controlled by two complementary genes, Rc and Rd. The RcRd genotype produces red pericarp in wild species Oryza rufipogon, whereas most cultivated rice varieties produce white grains resulted from a 14-bp frame-shift deletion in the seventh exon of the Rc gene. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13125
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http://dx.doi.org/10.1111/pbi.13125DOI Listing
April 2019
13 Reads

A gamma-thionin protein from apple, MdD1, is required for defense against S-RNase-induced inhibition of pollen tube prior to self / non-self recognition.

Plant Biotechnol J 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.

Apple exhibits S-RNase-mediated self-incompatibility. Although the cytotoxic effect of S-RNase inside the self-pollen tube has been studied extensively, the underlying defense mechanism in pollen tube in Rosaceae remains unclear. On exposure to stylar S-RNase plant defense responses are activated in the pollen tube, however how these are regulated is currently poorly understood. Read More

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http://dx.doi.org/10.1111/pbi.13131DOI Listing

PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2.

Plant Biotechnol J 2019 Apr 12. Epub 2019 Apr 12.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

In the present study, PeSTZ1, a cysteine-2/histidine-2-type zinc finger transcription factor, was isolated from the desert poplar, Populus euphratica, which serves as a model stress adaptation system for trees. PeSTZ1 was preferentially expressed in the young stems and was significantly up-regulated during chilling and freezing treatments. PeSTZ1 was localized to the nucleus and bound specifically to the PeAPX2 promoter. Read More

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http://dx.doi.org/10.1111/pbi.13130DOI Listing
April 2019
1 Read

Drought-responsive genes, late embryogenesis abundant group3 (LEA3) and vicinal oxygen chelate (VOC), function in lipid accumulation in Brassica napus and Arabidopsis mainly via enhancing photosynthetic efficiency and reducing ROS.

Plant Biotechnol J 2019 Apr 10. Epub 2019 Apr 10.

Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China, 430074.

Drought is an abiotic stress that affects plant growth, and lipids are the main economic factor in the agricultural production of oil crops. However, the molecular mechanisms of drought response function in lipid metabolism remain little known. In this study, overexpression (OE) of different copies of the drought response genes LEA3 and VOC enhanced both drought tolerance and oil content in Brassica napus and Arabidopsis. Read More

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http://dx.doi.org/10.1111/pbi.13127DOI Listing

Efficient genetic transformation and CRISPR/Cas9-mediated genome editing in Lemna aequinoctialis.

Plant Biotechnol J 2019 Apr 10. Epub 2019 Apr 10.

Key Laboratory of Biofuels, Qingdao Engineering Research Center of Biomass Resources and Environment, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of SciencesQingdao, China.

The fast growth, ease of metabolic labeling and potential for feedstock and biofuels production make duckweeds not only an attractive model system for understanding plant biology, but also a potential future crop. However, current duckweed research is constrained by the lack of efficient genetic manipulation tools. Here, we report a case study on genome-editing in a duckweed species, Lemna aequinoctialis, using a fast and efficient transformation and CRISPR/Cas9 tool. Read More

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http://dx.doi.org/10.1111/pbi.13128DOI Listing

Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust.

Plant Biotechnol J 2019 Apr 10. Epub 2019 Apr 10.

Synergetic Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.

Southern corn rust (SCR), which is a destructive disease caused by Puccinia polysora Underw. (P. polysora), commonly occurs in warm-temperate and tropical regions. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13129
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http://dx.doi.org/10.1111/pbi.13129DOI Listing
April 2019
8 Reads
5.752 Impact Factor

BBX16, a B-box protein, positively regulates light-induced anthocyanin accumulation by activating MYB10 in red pear.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Department of Horticulture, Zhejiang University, Hangzhou, China.

The red coloration of pear (Pyrus pyrifolia) results from anthocyanin accumulation in the fruit peel. Light is required for anthocyanin biosynthesis in pear. A pear homolog of Arabidopsis thaliana BBX22, PpBBX16, was differentially expressed after fruits were removed from bags and may be involved in anthocyanin biosynthesis. Read More

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http://dx.doi.org/10.1111/pbi.13114DOI Listing
April 2019
2 Reads

Optimizing Base Editors for Improved Efficiency and Expanded Editing Scope in Rice.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.

Base editors, presently including cytidine base editors (CBEs) and adenine base editors (ABEs), enable precise base alterations in the genome without inducing DNA double-stranded breaks (DSBs). Base editors are valuable tools for precision plant molecular breeding since many agronomic traits are controlled by variations in one or few DNA bases. The early developed CBE and ABE systems, consisting of the rat cytidine deaminase APOBEC1 (rAPOBEC1) or activation-induced cytidine deaminase (AID) PmCDA1, and the evolved tRNA adenine deaminase TadA, respectively, have been applied to many plant species. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13124
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http://dx.doi.org/10.1111/pbi.13124DOI Listing
April 2019
8 Reads

Validation of leaf enzymes in the detergent and textile industries: launching of a new platform technology.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Chemical catalysts are being replaced by biocatalysts in almost all industrial applications due to environmental concerns, thereby increasing their demand. Enzymes in current industries are produced in microbial systems or plant seeds. We report here five newly launched leaf-enzyme products and their validation with 15 commercial microbial-enzyme products, for detergent or textile industries. Read More

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http://dx.doi.org/10.1111/pbi.13122DOI Listing
April 2019
1 Read
5.752 Impact Factor

Genome-wide association study of six quality traits reveals the association of the TaRPP13L1 gene with flour colour in Chinese bread wheat.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops/Agronomy College, Henan Agricultural University, Zhengzhou, China.

Flour colour, kernel hardness, grain protein content and wet gluten content are important quality properties that determine end use in bread wheat. Here, a wheat 90K genotyping assay was used for a genome-wide association study (GWAS) of the six quality-related traits in Chinese wheat cultivars in eight environments over four years. A total of 846 significant single nucleotide polymorphisms (SNPs) were identified, explaining approximately 30% of the phenotypic variation on average, and 103 multienvironment-significant SNPs were detected in more than four environments. Read More

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http://dx.doi.org/10.1111/pbi.13126DOI Listing
April 2019
1 Read

FtMYB16 interacts with Ftimportin-α1 to regulate rutin biosynthesis in tartary buckwheat.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Fagopyrum tataricum is an ancient dicotyledonous crop and is well known to enrich in flavonoids, such as rutin. The Subgroup 4 of R2R3-MYB transcription factors, FtMYB11, FtMYB13, FtMYB14, FtMYB15 and FtMYB16 are key repressors of rutin biosynthesis in buckwheat. However, the regulatory mechanism of FtMYB16 on rutin biosynthesis is still unclear. Read More

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http://dx.doi.org/10.1111/pbi.13121DOI Listing
April 2019
2 Reads

Validation of leaf and microbial pectinases: commercial launching of a new platform technology.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Almost all current genetically modified plant commercial products are derived from seeds. The first protein product made in leaves is made for commercial use is reported here. Leaf-pectinases are validated here with eight liquid commercial microbial-enzyme products for textile or juice industry applications. Read More

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http://dx.doi.org/10.1111/pbi.13119DOI Listing

Cas9-PF, an early flowering and visual selection marker system, enhances the frequency of editing event occurrence and expedites the isolation of genome-edited and transgene-free plants.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, 650021, China.

Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-mediated gene editing technology has been successfully and widely used in plants due to its ability to provide expedient and efficient mutagenesis with unprecedented precision (Ma et al., 2016). Although several cases have shown that mutant plants can be generated via transient transformation or DNA-free editing (Liang et al. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13118
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http://dx.doi.org/10.1111/pbi.13118DOI Listing
April 2019
5 Reads

An adenine base editor with expanded targeting scope using SpCas9-NGv1 in rice.

Plant Biotechnol J 2019 Apr 8. Epub 2019 Apr 8.

Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.

The CRISPR/Cas9 genome editing system induces mainly small insertions or deletions at the target site. In contrast, base editors are precise genome editing tools that enable base conversion at a target site without inducing a DNA double-strand break. Two types of base editors have now been developed: cytosine base editors (CBEs)-cytidine deaminase fused with catalytically impaired Cas9-can efficiently convert a C-G base pair to a T-A pair, while adenine base editors (ABEs)-Cas9 nickase (nCas9) plus engineered Escherichia coli adenosine deaminase (TadA)-enable A-T to G-C conversions (Gaudelli et al. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13120
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http://dx.doi.org/10.1111/pbi.13120DOI Listing
April 2019
8 Reads

RrMYB5- and RrMYB10-regulated flavonoid biosynthesis plays a pivotal role in feedback loop responding to wounding and oxidation in Rosa rugosa.

Plant Biotechnol J 2019 Apr 5. Epub 2019 Apr 5.

Key laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Flavonoids play critical roles in plant responses to various stresses. Few studies have been reported on what the mechanism of activating flavonoid biosynthesis in plant responses to wounding and oxidation is. In this study, flavonoid metabolites and many MYB transcript factors from Rosa rugosa were verified to be induced by wounding and oxidation. Read More

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http://dx.doi.org/10.1111/pbi.13123DOI Listing
April 2019
5.752 Impact Factor

A directed learning strategy integrating multiple omic data improves genomic prediction.

Plant Biotechnol J 2019 Apr 5. Epub 2019 Apr 5.

Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.

Genomic prediction (GP) aims to construct a statistical model for predicting phenotypes using genome-wide markers and is a promising strategy for accelerating molecular plant breeding. However, current progress of phenotype prediction using genomic data alone has reached a bottleneck, and previous studies on transcriptomic and metabolomic predictions ignored genomic information. Here, we designed a novel strategy of GP called multilayered least absolute shrinkage and selection operator (MLLASSO) by integrating multiple omic data into a single model that iteratively learns three layers of genetic features (GFs) supervised by observed transcriptome and metabolome. Read More

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http://dx.doi.org/10.1111/pbi.13117DOI Listing

Comparative population genomics identified genomic regions and candidate genes associated with fruit domestication traits in peach.

Plant Biotechnol J 2019 Apr 5. Epub 2019 Apr 5.

The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Fruit Tree Breeding Technology), Ministry of Agriculture, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Crop evolution is a long-term process involving selection by natural evolutionary forces and anthropogenic influences; however, the genetic mechanisms underlying the domestication and improvement of fruit crops have not been well studied to date. Here, we performed a population structure analysis in peach (Prunus persica) based on the genome-wide resequencing of 418 accessions and confirmed the presence of an obvious domestication event during evolution. We identified 132 and 106 selective sweeps associated with domestication and improvement, respectively. Read More

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http://dx.doi.org/10.1111/pbi.13112DOI Listing
April 2019
1 Read

Assessment of Cas12a-mediated gene editing efficiency in plants.

Plant Biotechnol J 2019 Apr 5. Epub 2019 Apr 5.

Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Valencia, Spain.

The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering. To obtain a comparative assessment of RNA-guided endonuclease (RGEN) types in plants, we adapted the CRISPR/Cas12a system to the GoldenBraid (GB) modular cloning platform and compared the efficiency of Acidaminococcus (As) and Lachnospiraceae (Lb) Cas12a variants with the previously described GB-assembled Streptococcus pyogenes Cas9 (SpCas9) constructs in eight Nicotiana benthamiana loci using transient expression. All three nucleases showed drastic target-dependent differences in efficiency, with LbCas12 producing higher mutagenesis rates in five of the eight loci assayed, as estimated with the T7E1 endonuclease assay. Read More

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http://dx.doi.org/10.1111/pbi.13113DOI Listing
April 2019
1 Read

MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants.

Plant Biotechnol J 2019 Apr 4. Epub 2019 Apr 4.

Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.

MicroRNAs (miRNAs) modulate the abundance and spatial-temporal accumulation of target mRNAs and indirectly regulate several plant processes. Transcriptional regulation of the genes encoding miRNAs (MIR genes) can be activated by numerous transcription factors, which themselves are regulated by other miRNAs. Fine-tuning of MIR genes or miRNAs is a powerful biotechnological strategy to improve tolerance to abiotic or biotic stresses in crops of economic importance. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13116
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http://dx.doi.org/10.1111/pbi.13116DOI Listing
April 2019
13 Reads

Genome-wide selection footprints and deleterious variations in young Asian allotetraploid rapeseed.

Plant Biotechnol J 2019 Apr 4. Epub 2019 Apr 4.

Institute of Crop Sciences & Institute of Bioinformatics, Zhejiang University, Hangzhou, China.

Brassica napus (AACC, 2n = 38) is an important oilseed crop grown worldwide. However, little is known about the population evolution of this species, the genomic difference between its major genetic groups, such as European and Asian rapeseed, and the impacts of historical large-scale introgression events on this young tetraploid. In this study, we reported the de novo assembly of the genome sequences of an Asian rapeseed (B. Read More

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http://dx.doi.org/10.1111/pbi.13115DOI Listing

Tea Plant Information Archive: a comprehensive genomics and bioinformatics platform for tea plant.

Plant Biotechnol J 2019 Mar 26. Epub 2019 Mar 26.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, China.

Tea is the world's widely consumed nonalcohol beverage with essential economic and health benefits. Confronted with the increasing large-scale omics-data set particularly the genome sequence released in tea plant, the construction of a comprehensive knowledgebase is urgently needed to facilitate the utilization of these data sets towards molecular breeding. We hereby present the first integrative and specially designed web-accessible database, Tea Plant Information Archive (TPIA; http://tpia. Read More

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http://dx.doi.org/10.1111/pbi.13111DOI Listing
March 2019
5.752 Impact Factor

CRISPR-LbCas12a-mediated modification of citrus.

Plant Biotechnol J 2019 Mar 25. Epub 2019 Mar 25.

Department of Microbiology and Cell Science, Citrus Research and Education Center, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, USA.

Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully in Duncan grapefruit via Xcc-facilitated agroinfiltration. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13109
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http://dx.doi.org/10.1111/pbi.13109DOI Listing
March 2019
12 Reads

The potential of plant systems to break the HIV-TB link.

Plant Biotechnol J 2019 Mar 25. Epub 2019 Mar 25.

Embrapa Genetic Resources and Biotechnology, PqEB-Final W5 Norte - CP 02372, Brasília-DF, Brazil.

Tuberculosis (TB) and human immunodeficiency virus (HIV) can place a major burden on healthcare systems and constitute the main challenges of diagnostic and therapeutic programs. Infection with HIV is the most common cause of Mycobacterium tuberculosis (Mtb), which can accelerate the risk of latent TB reactivation by 20-fold. Similarly, TB is considered the most relevant factor predisposing individuals to HIV infection. Read More

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http://dx.doi.org/10.1111/pbi.13110DOI Listing
March 2019
1 Read

FLOURY ENDOSPERM16 encoding a NAD-dependent cytosolic malate dehydrogenase plays an important role in starch synthesis and seed development in rice.

Plant Biotechnol J 2019 Mar 12. Epub 2019 Mar 12.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.

Starch is the most important form of energy storage in cereal crops. Many key enzymes involved in starch biosynthesis have been identified. However, the molecular mechanisms underlying the regulation of starch biosynthesis are largely unknown. Read More

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http://dx.doi.org/10.1111/pbi.13108DOI Listing

SELTP assembled battery drives totipotency of somatic plant cell.

Plant Biotechnol J 2019 Mar 7. Epub 2019 Mar 7.

State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, P. R. China.

Plants display a remarkable capacity for somatic cell totipotency, as demonstrated by single plant cells that can develop into embryos and complete plants. How does a single somatic cell become a whole plant? This represents the forefront of the most compelling puzzles since Haberlandt's prediction of totipotency (1902) and is one of the top 25 big questions that face scientific inquiry today (Vogel, 2005). Elucidation of somatic cell totipotency is of great fundamental importance. Read More

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http://dx.doi.org/10.1111/pbi.13107DOI Listing

CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production.

Plant Biotechnol J 2019 Mar 6. Epub 2019 Mar 6.

School of Agriculture, Food & Wine, The University of Adelaide, Urrbrae, South Australia, Australia.

The development and adoption of hybrid seed technology have led to dramatic increases in agricultural productivity. However, it has been a challenge to develop a commercially viable platform for the production of hybrid wheat (Triticum aestivum) seed due to wheat's strong inbreeding habit. Recently, a novel platform for commercial hybrid seed production was described. Read More

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http://doi.wiley.com/10.1111/pbi.13106
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http://dx.doi.org/10.1111/pbi.13106DOI Listing
March 2019
3 Reads

A wheat NAC interacts with an orphan protein and enhances resistance to Fusarium Head Blight disease.

Plant Biotechnol J 2019 Mar 1. Epub 2019 Mar 1.

UCD, School of Biology and Environmental Science and Earth Institute, College of Science, University College Dublin, Belfield, Dublin 4, Ireland.

Taxonomically-restricted orphan genes play an important role in environmental adaptation, as recently demonstrated by the fact that the Pooideae-specific orphan TaFROG (Triticum aestivum Fusarium Resistance Orphan Gene) enhanced wheat resistance to the economically devastating Fusarium head blight (FHB) disease. Like most orphan genes, little is known about the cellular function of the encoded protein TaFROG, other than it interacts with the central stress regulator TaSnRK1α. Here, we functionally characterized a wheat (Triticum aestivum) NAC-like transcription factor TaNACL-D1 that interacts with TaFROG and investigated its' role in FHB using studies to assess motif analyses, yeast transactivation, protein-protein interaction, gene expression and the disease response of wheat lines overexpressing TaNACL-D1. Read More

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http://dx.doi.org/10.1111/pbi.13105DOI Listing

Reducing expression of a nitrate-responsive bZIP transcription factor increases grain yield and N use in wheat.

Plant Biotechnol J 2019 Feb 27. Epub 2019 Feb 27.

The State Key Laboratory for Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Nitrogen (N) plays critical role in plant growth; manipulating N assimilation could be a target to increase grain yield and N use. Here, we show that ABRE-binding factor (ABF)-like leucine zipper transcription factor TabZIP60 mediates N use and growth in wheat. The expression of TabZIP60 is repressed when the N-deprived wheat plants is exposed to nitrate. Read More

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http://dx.doi.org/10.1111/pbi.13103DOI Listing
February 2019
3 Reads

The bZIP73 transcription factor controls rice cold tolerance at the reproductive stage.

Plant Biotechnol J 2019 Feb 27. Epub 2019 Feb 27.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Cold temperature during the reproductive stage often causes great yield loss of grain crops in subtropical and temperate regions. Previously we showed that the rice transcription factor bZIP73 plays an important role in cold adaptation at the seedling stage. Here we further demonstrate that bZIP73 also confers cold stress tolerance at the reproductive stage. Read More

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http://dx.doi.org/10.1111/pbi.13104DOI Listing
February 2019
5.752 Impact Factor

Development of an efficient root transgenic system for pigeon pea and its application to other important economically plants.

Plant Biotechnol J 2019 Feb 25. Epub 2019 Feb 25.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.

For non-model plants, functional characterization of genes is still hampered by lack of efficient stable transformation procedures. Here, we report a simple, fast and efficient transformation technique with Agrobacterium rhizogenes for generating stable transgenic roots in living plants to facilitate functional studies in vivo. We showed that injection of A. Read More

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http://dx.doi.org/10.1111/pbi.13101DOI Listing
February 2019
5 Reads

Generation of virus-resistant potato plants by RNA genome targeting.

Plant Biotechnol J 2019 Feb 25. Epub 2019 Feb 25.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA-targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y (PVY). Read More

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http://doi.wiley.com/10.1111/pbi.13102
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http://dx.doi.org/10.1111/pbi.13102DOI Listing
February 2019
5 Reads

Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants.

Plant Biotechnol J 2019 Feb 22. Epub 2019 Feb 22.

Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium.

Plant expression systems have proven to be exceptional in producing high-value complex polymeric proteins such as secretory IgAs (SIgAs). However, polymeric protein production requires the expression of multiple genes, which can be transformed as single or multiple T-DNA units to generate stable transgenic plant lines. Here, we evaluated four strategies to stably transform multiple genes and to obtain high expression of all components. Read More

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http://dx.doi.org/10.1111/pbi.13098DOI Listing
February 2019

The genetic basis of drought tolerance in the high oil crop Sesamum indicum.

Plant Biotechnol J 2019 Feb 22. Epub 2019 Feb 22.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, Hubei, China.

Unlike most of the important food crops, sesame can survive drought but severe and repeated drought episodes, especially occurring during the reproductive stage, significantly curtail the productivity of this high oil crop. Genome-wide association study was conducted for traits related to drought tolerance using 400 diverse sesame accessions, including landraces and modern cultivars. Ten stable QTLs explaining more than 40% of the phenotypic variation and located on four linkage groups were significantly associated with drought tolerance related traits. Read More

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http://doi.wiley.com/10.1111/pbi.13100
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http://dx.doi.org/10.1111/pbi.13100DOI Listing
February 2019
8 Reads

A WRKY transcription factor PbrWRKY53 from Pyrus betulaefolia is involved in drought tolerance and AsA accumulation.

Plant Biotechnol J 2019 Feb 22. Epub 2019 Feb 22.

College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

WRKY comprises a large family of transcription factors in plants, but most WRKY members are still poorly understood. In this study, we report the identification and functional characterization of PbrWRKY53 isolated from Pyrus betulaefolia. PbrWRKY53 was greatly up-regulated by drought and abscisic acid, but slightly induced by salt and cold. Read More

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http://dx.doi.org/10.1111/pbi.13099DOI Listing
February 2019

Immunogenicity of plant-produced porcine circovirus-like particles in mice.

Plant Biotechnol J 2019 Feb 21. Epub 2019 Feb 21.

Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa.

Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expression systems are increasingly regarded as a viable technology to produce recombinant proteins for use as pharmaceutical agents and vaccines. Read More

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http://dx.doi.org/10.1111/pbi.13097DOI Listing
February 2019
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Establishing CRISPR/Cas13a immune system conferring RNA virus resistance in both dicot and monocot plants.

Plant Biotechnol J 2019 Feb 20. Epub 2019 Feb 20.

Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.

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http://doi.wiley.com/10.1111/pbi.13095
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http://dx.doi.org/10.1111/pbi.13095DOI Listing
February 2019
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Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses.

Plant Biotechnol J 2019 Feb 19. Epub 2019 Feb 19.

GAFL, INRA, Montfavet, France.

In many crop species, natural variation in eIF4E proteins confers resistance to potyviruses. Gene editing offers new opportunities to transfer genetic resistance to crops that seem to lack natural eIF4E alleles. However, because eIF4E are physiologically important proteins, any introduced modification for virus resistance must not bring adverse phenotype effects. Read More

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http://dx.doi.org/10.1111/pbi.13096DOI Listing
February 2019
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A single nucleotide mutation in GID1c disrupts its interaction with DELLA1 and causes a GA-insensitive dwarf phenotype in peach.

Plant Biotechnol J 2019 Feb 18. Epub 2019 Feb 18.

College of Horticulture, Henan Agricultural University, Zhengzhou, China.

Plant stature is one important factor that affects the productivity of peach orchards. However, little is known about the molecular mechanism(s) underlying the dwarf phenotype of peach tree. Here, we report a dwarfing mechanism in the peach cv. Read More

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http://dx.doi.org/10.1111/pbi.13094DOI Listing
February 2019

Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice.

Plant Biotechnol J 2019 Feb 16. Epub 2019 Feb 16.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, China.

Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)-like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35), and cloning of the causal gene by TAIL-PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H O , up-regulated expression of defence-related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. Read More

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http://dx.doi.org/10.1111/pbi.13093DOI Listing
February 2019
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Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana.

Plant Biotechnol J 2019 Feb 11. Epub 2019 Feb 11.

Department of Plant Sciences, Plant Chemetics Laboratory, University of Oxford, Oxford, UK.

Co-expression of protease inhibitors like the tomato cystatin SlCYS8 is useful to increase recombinant protein production in plants, but key proteases involved in protein proteolysis are still unknown. Here, we performed activity-based protein profiling to identify proteases that are inhibited by SlCYS8 in agroinfiltrated Nicotiana benthamiana. We discovered that SlCYS8 selectively suppresses papain-like cysteine protease (PLCP) activity in both apoplastic fluids and total leaf extracts, while not affecting vacuolar-processing enzyme and serine hydrolase activity. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13092
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http://dx.doi.org/10.1111/pbi.13092DOI Listing
February 2019
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The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa).

Plant Biotechnol J 2019 Feb 7. Epub 2019 Feb 7.

Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Multi-functional microRNAs (miRNAs) are emerging as key modulators of plant-pathogen interactions. Although the involvement of some miRNAs in plant-insect interactions has been revealed, the underlying mechanisms are still elusive. The brown planthopper (BPH) is the most notorious rice (Oryza sativa)-specific insect that causes severe yield losses each year and requires urgent biological control. Read More

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http://doi.wiley.com/10.1111/pbi.13091
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http://dx.doi.org/10.1111/pbi.13091DOI Listing
February 2019
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A novel anti-HIV-1 bispecific bNAb-lectin fusion protein engineered in a plant-based transient expression system.

Plant Biotechnol J 2019 Feb 6. Epub 2019 Feb 6.

James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.

The discovery of broadly neutralizing antibodies (bNAbs) has been a major step towards better prophylactic and therapeutic agents against human immunodeficiency virus type 1 (HIV-1). However, effective therapy will likely require a combination of anti-HIV agents to avoid viral evasion. One possible solution to this problem is the creation of bispecific molecules that can concurrently target two vulnerable sites providing synergistic inhibitory effects. Read More

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http://dx.doi.org/10.1111/pbi.13090DOI Listing
February 2019
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High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.

Plant Biotechnol J 2019 Feb 1. Epub 2019 Feb 1.

Agricultural Division of Dow DuPont, Corteva Agriscience™, Johnston, IA, USA.

An efficient Agrobacterium-mediated site-specific integration (SSI) technology using the flipase/flipase recognition target (FLP/FRT) system in elite maize inbred lines is described. The system allows precise integration of a single copy of a donor DNA flanked by heterologous FRT sites into a predefined recombinant target line (RTL) containing the corresponding heterologous FRT sites. A promoter-trap system consisting of a pre-integrated promoter followed by an FRT site enables efficient selection of events. Read More

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http://doi.wiley.com/10.1111/pbi.13089
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http://dx.doi.org/10.1111/pbi.13089DOI Listing
February 2019
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Development of an Agrobacterium-delivered CRISPR/Cas9 system for wheat genome editing.

Plant Biotechnol J 2019 Feb 1. Epub 2019 Feb 1.

Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA.

CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA delivery methods. In wheat, the biolistics based transformation is the most used method for delivery of the CRISPR/Cas9 complex. Read More

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http://doi.wiley.com/10.1111/pbi.13088
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http://dx.doi.org/10.1111/pbi.13088DOI Listing
February 2019
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Haplotype analysis of key genes governing grain yield and quality traits across 3K RG panel reveals scope for the development of tailor-made rice with enhanced genetic gains.

Plant Biotechnol J 2019 Jan 30. Epub 2019 Jan 30.

International Rice Research Institute, South-Asia Hub, ICRISAT Campus, Hyderabad, India.

Though several genes governing various major traits have been reported in rice, their superior haplotype combinations for developing ideal variety remains elusive. In this study, haplotype analysis of 120 previously functionally characterized genes, influencing grain yield (87 genes) and grain quality (33 genes) revealed significant variations in the 3K rice genome (RG) panel. For selected genes, meta-expression analysis using already available datasets along with co-expression network provided insights at systems level. Read More

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http://dx.doi.org/10.1111/pbi.13087DOI Listing
January 2019
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Development of an integrated 200K SNP genotyping array and application for genetic mapping, genome assembly improvement and genome wide association studies in pear (Pyrus).

Plant Biotechnol J 2019 Jan 28. Epub 2019 Jan 28.

Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

Pear (Pyrus; 2n = 34), the third most important temperate fruit crop, has great nutritional and economic value. Despite the availability of many genomic resources in pear, it is challenging to genotype novel germplasm resources and breeding progeny in a timely and cost-effective manner. Genotyping arrays can provide fast, efficient and high-throughput genetic characterization of diverse germplasm, genetic mapping and breeding populations. Read More

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http://doi.wiley.com/10.1111/pbi.13085
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http://dx.doi.org/10.1111/pbi.13085DOI Listing
January 2019
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Whole-genome re-sequencing reveals the impact of the interaction of copy number variants of the rhg1 and Rhg4 genes on broad-based resistance to soybean cyst nematode.

Plant Biotechnol J 2019 Jan 28. Epub 2019 Jan 28.

Division of Plant Sciences, University of Missouri, Columbia, MO, USA.

Soybean cyst nematode (SCN) is the most devastating plant-parasitic nematode. Most commercial soybean varieties with SCN resistance are derived from PI88788. Resistance derived from PI88788 is breaking down due to narrow genetic background and SCN population shift. Read More

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http://dx.doi.org/10.1111/pbi.13086DOI Listing
January 2019
1 Read