40,321 results match your criteria Pigment Cell & Melanoma Research[Journal]


Cyanidin-3-glucoside induces mesenchymal to epithelial transition via activating Sirt1 expression in triple negative breast cancer cells.

Biochimie 2019 Mar 12. Epub 2019 Mar 12.

Department of Clinical Nutrition, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China. Electronic address:

Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis. Cyanidin-3-glucoside (C3G), the most abundant anthocyanin pigment enriched in fresh fruits and vegetables, showed ideal anti-oxidant property. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.004DOI Listing

ERBB2-modulated ATG4B and autophagic cell death in human ARPE19 during oxidative stress.

PLoS One 2019 14;14(3):e0213932. Epub 2019 Mar 14.

School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan.

Age-related macular degeneration (AMD) is an ocular disease with retinal degeneration. Retinal pigment epithelium (RPE) degeneration is mainly caused by long-term oxidative stress. Kinase activity could be either protective or detrimental to cells during oxidative stress; however, few reports have described the role of kinases in oxidative stress. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0213932PLOS
March 2019
2 Reads

Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4 improves diabetic retinopathy.

Biosci Biotechnol Biochem 2019 Mar 14:1-5. Epub 2019 Mar 14.

a Department of Endocrinology , The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , Hubei , P. R. China.

Diabetes induced a serious of complications including diabetic retinopathy. Our study aimed to investigate the role of Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4 in diabetic retinopathy. A mice model of diabetic retinopathy was established, and expression of SDF-1 and CXCR4 in retina was examined by Real-time quantitative PCR (qRT-PCR). Read More

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http://dx.doi.org/10.1080/09168451.2019.1588095DOI Listing

In vivo expansion and regeneration of full-thickness functional skin with an autologous homologous skin construct: Clinical proof of concept for chronic wound healing.

Int Wound J 2019 Mar 13. Epub 2019 Mar 13.

Department of Research and Development, PolarityTE, Inc., Salt Lake City, Utah.

A new cell-tissue technology uses a patient's skin to create an in vivo expanding and self-organising full-thickness skin autograft derived from potent cutaneous appendages. This autologous homologous skin construct (AHSC) is manufactured from a small full-thickness skin harvest obtained from an uninjured area of the patient. All the harvested tissue is incorporated into the AHSC including the endogenous regenerative cellular populations responsible for skin maintenance and repair, which are activated during the manufacturing process. Read More

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http://dx.doi.org/10.1111/iwj.13109DOI Listing

Abnormal mTORC1 signaling leads to retinal pigment epithelium degeneration.

Theranostics 2019 30;9(4):1170-1180. Epub 2019 Jan 30.

Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Retinal pigment epithelial (RPE) degeneration is potentially involved in the pathogenesis of several retinal degenerative diseases. mTORC1 signaling is shown as a crucial regulator of many biological processes and disease progression. In this study, we aimed at investigating the role of mTORC1 signaling in RPE degeneration. Read More

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http://dx.doi.org/10.7150/thno.26281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401408PMC
January 2019

Biological detoxification of Monascus purpureus pigments by heat treated Saccharomyces cerevisiae.

J Sci Food Agric 2019 Mar 13. Epub 2019 Mar 13.

Department of Chemistry, Payame Noor University, Mashhad, Iran.

Background: Today, there is an increasing concern about the consumption of synthetic colorants in food because of their possible health hazards. Monascus purpureus have attracted a great deal of attention as they produce various colored pigments with high chemical stability but they produces citrinin, a secondary toxic metabolite, along with the pigments. This study aims to investigate the amount of pigment and citrinin reduction by different treatments of Saccharomyces cerevisiae such as heat treatment and suspension concentration. Read More

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http://doi.wiley.com/10.1002/jsfa.9680
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http://dx.doi.org/10.1002/jsfa.9680DOI Listing
March 2019
1 Read

Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine.

PLoS One 2019 13;14(3):e0212369. Epub 2019 Mar 13.

Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, Japan.

Regenerative medicine has received a lot of attention as a novel strategy for injuries and diseases that are difficult to cure using current techniques. Cell production, which is vital for regenerative medicine, has undergone remarkable progress via breakthroughs in developmental biology and tissue engineering; currently, cell production requires numerous experimental operators performing manual, small-scale cell cultures. Other major obstacles for cell production and regenerative medicine include the variable quality of products based on the experimental procedure, the skills of operators, the level of labor required for production, and costs. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212369PLOS

Impaired ABCA1/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration.

Elife 2019 Mar 13;8. Epub 2019 Mar 13.

Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland.

Age-related macular degeneration (AMD) is a progressive disease of the retinal pigment epithelium (RPE) and the retina leading to loss of central vision. Polymorphisms in genes involved in lipid metabolism, including the ATP-binding cassette transporter A1 (), have been associated with AMD risk. However, the significance of retinal lipid handling for AMD pathogenesis remains elusive. Read More

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https://elifesciences.org/articles/45100
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http://dx.doi.org/10.7554/eLife.45100DOI Listing
March 2019
2 Reads

PEDF decreases cardiomyocyte edema during oxygen‑glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1.

Int J Mol Med 2019 Mar 12. Epub 2019 Mar 12.

Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China.

Myocardial edema is divided into cellular edema and interstitial edema; however, the dynamic change of cardiomyocyte edema has not been described in detail. Pigment epithelium‑derived factor (PEDF) is known for its protective effects on ischemic cardiomyocytes; however, the association between PEDF and cardiomyocyte edema remains to be fully elucidated. In the present study, rat neonatal left ventricular cardiomyocytes were isolated and treated with oxygen‑glucose deprivation (OGD) and recovery. Read More

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http://dx.doi.org/10.3892/ijmm.2019.4132DOI Listing

Transcriptome analysis of the curry tree (Bergera koenigii L., Rutaceae) during leaf development.

Sci Rep 2019 Mar 12;9(1):4230. Epub 2019 Mar 12.

Department of Botany and Laboratories of Analytical Biology, National Museum of Natural History, Smithsonian Institution, PO Box 37012, Washington, DC, 20013-7012, USA.

The curry tree (Bergera koenigii L.) is a widely cultivated plant used in South Asian cooking. Next-generation sequencing was used to generate the transcriptome of the curry leaf to detect changes in gene expression during leaf development, such as those genes involved in the production of oils which lend the leaf its characteristic taste, aroma, and medicinal properties. Read More

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http://dx.doi.org/10.1038/s41598-019-40227-zDOI Listing

Homozygous frameshift mutations in FAT1 cause a syndrome characterized by colobomatous-microphthalmia, ptosis, nephropathy and syndactyly.

Nat Commun 2019 Mar 12;10(1):1180. Epub 2019 Mar 12.

Centre de Recherche en Génomique des Pathologies Humaines (GENOPATH), Faculté de Médecine et de Pharmacie, Mohammed V University of Rabat, 10100, Rabat, Morocco.

A failure in optic fissure fusion during development can lead to blinding malformations of the eye. Here, we report a syndrome characterized by facial dysmorphism, colobomatous microphthalmia, ptosis and syndactyly with or without nephropathy, associated with homozygous frameshift mutations in FAT1. We show that Fat1 knockout mice and zebrafish embryos homozygous for truncating fat1a mutations exhibit completely penetrant coloboma, recapitulating the most consistent developmental defect observed in affected individuals. Read More

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http://dx.doi.org/10.1038/s41467-019-08547-wDOI Listing

Amphiregulin and ocular axial length.

Acta Ophthalmol 2019 Mar 12. Epub 2019 Mar 12.

Department of Ophthalmology, Medical Faculty Mannheim of the Ruprecht-Karls-University Heidelberg, Mannheim, Germany.

Purpose: To assess the potential role of amphiregulin as messenger molecule in ocular axial elongation.

Methods: The experimental study included guinea pigs (total n = 78) (age: 3-4 weeks) which underwent bilateral lens-induced myopization and received 15 days later three intraocular injections in weekly intervals of amphiregulin antibody (doses:5 μg, 10 μg, 20 μg) into their right eyes, and three phosphate-buffered saline injections into their left eyes; and guinea pigs without lens-induced myopization and which received three unilateral intraocular injections of amphiregulin antibody (dose: 20 μg) or amphiregulin (doses: 1 ng; 10 ng; 20 ng) into their right eyes, and three phosphate-buffered saline injections into their left eyes. Seven days later, the animals were sacrificed. Read More

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http://doi.wiley.com/10.1111/aos.14080
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http://dx.doi.org/10.1111/aos.14080DOI Listing
March 2019
7 Reads

Assessment of Rhodopirellula rubra as a supplementary and nutritional food source to the microcrustacean Daphnia magna.

Antonie Van Leeuwenhoek 2019 Mar 11. Epub 2019 Mar 11.

Departamento de Biologia da Faculdade de Ciências da Universidade do Porto (FCUP), Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.

The daily use of the planctomycete Rhodopirellula rubra as an alternative or supplementary food source for Daphnia magna and its feasibility in the nutrition of transgenerational populations were studied. The life history parameters, fatty acids (saturated, mono- and polyunsaturated; SFAs, MUFAs and PUFAs), glycogen and protein contents of organisms during feeding assays and of the first generation were analysed. An increase in the yields of D. Read More

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http://dx.doi.org/10.1007/s10482-019-01255-xDOI Listing

Amine derivatives of furocoumarin induce melanogenesis by activating Akt/GSK-3β/β-catenin signal pathway.

Drug Des Devel Ther 2019 12;13:623-632. Epub 2019 Feb 12.

Key Laboratory of Plant Resources and Chemistry in Arid Regions, State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China,

Background: Melanogenesis, or the biosynthesis of melanin, plays a critical role in the pigmentation of skin, hair, and eyes. Reduced melanogenesis may lead to depigmentation conditions such as vitiligo. Psoralen, a furocoumarin derivative, is closely associated with melanogenesis, and its derivative 8-methoxypsoralen is used in psoralen and ultraviolet A therapy for pigmentation disorders. Read More

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http://dx.doi.org/10.2147/DDDT.S180960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387609PMC
February 2019

Volatile Evolution of Long Non-Coding RNA Repertoire in Retinal Pigment Epithelium: Insights from Comparison of Bovine and Human RNA Expression Profiles.

Genes (Basel) 2019 Mar 8;10(3). Epub 2019 Mar 8.

Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Currently, several long non-coding RNAs (lncRNAs) (TUG1, MALAT1, MEG3 and others) have been discovered to regulate normal visual function and may potentially contribute to dysfunction of the retina. We decided to extend these analyses of lncRNA genes to the retinal pigment epithelium (RPE) to determine whether there is conservation of RPE-expressed lncRNA between human and bovine genomes. We reconstructed bovine RPE lncRNAs based on genome-guided assembly. Read More

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http://dx.doi.org/10.3390/genes10030205DOI Listing

Tyrosinase inhibition and anti-melanin generation effect of cinnamamide analogues.

Bioorg Chem 2019 Mar 4;87:43-55. Epub 2019 Mar 4.

Laboratory of Medicinal Chemistry, College of Pharmacy, Pusan National University, Busan 46241, South Korea. Electronic address:

Abnormal melanogenesis results in excessive production of melanin, leading to pigmentation disorders. As a key and rate-limiting enzyme for melanogenesis, tyrosinase has been considered an important target for developing therapeutic agents of pigment disorders. Despite having an (E)-β-phenyl-α,β-unsaturated carbonyl scaffold, which plays an important role in the potent inhibition of tyrosinase activity, cinnamic acids have not attracted attention as potential tyrosinase inhibitors, due to their low tyrosinase inhibitory activity and relatively high hydrophilicity. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.001DOI Listing
March 2019
9 Reads

Biomarkers, measured during therapy, for response of melanoma patients to immune checkpoint inhibitors: a systematic review.

Melanoma Res 2019 Mar 7. Epub 2019 Mar 7.

Department of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam Infection and Immunity Institute, Cancer Center Amsterdam.

Immune checkpoint inhibitors (ICIs), which target CTLA-4 or PD-(L)1 molecules, have shown impressive therapeutic results. Durable responses, however, are only observed in a segment of the patient population and must be offset against severe off-target immune toxicity and high costs. This calls for biomarkers that predict response during ICI treatment. Read More

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http://dx.doi.org/10.1097/CMR.0000000000000589DOI Listing

Porphyrin Excretion Resulting From Mutation of a Gene Encoding a Class I Fructose 1,6-Bisphosphate Aldolase in .

Front Microbiol 2019 22;10:301. Epub 2019 Feb 22.

Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, Canada.

This paper describes a mutant (called SB1707) of the wild type strain SB1003 in which a transposon-disrupted gene resulted in a ∼25-fold increase in the accumulation of coproporphyrin III in the medium of phototrophic (anaerobic) cultures grown in a yeast extract/peptone medium. There was little or no stimulation of pigment accumulation in aerobic cultures. Therefore, this effect of mutation appears to be specific for the anaerobic coproporphyrinogen III oxidase HemN as opposed to the aerobic enzyme HemF. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395792PMC
February 2019

Oral Levodopa rescues retinal morphology and visual function in a murine model of human albinism.

Pigment Cell Melanoma Res 2019 Mar 9. Epub 2019 Mar 9.

Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Sir Henry Wellcome Laboratories, South Block Mail point 806, Level D, Southampton University Hospital, Southampton, SO16 6YD, UK.

Albinism is a group of disorders characterised by pigment deficiency and abnormal retinal development. Despite being a common cause for visual impairment world-wide, there is a paucity of treatments and patients typically suffer life-long visual disability. Residual plasticity of the developing retina in young children with albinism has been demonstrated, suggesting a post-natal window for therapeutic rescue. Read More

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http://dx.doi.org/10.1111/pcmr.12782DOI Listing
March 2019
1 Read

Fine particulate matter (PM2.5) inhibits ciliogenesis by increasing SPRR3 expression via c-Jun activation in RPE cells and skin keratinocytes.

Sci Rep 2019 Mar 8;9(1):3994. Epub 2019 Mar 8.

School of Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.

Exposure to fine particulate matter (PM) with diameter <2.5 µm (PM2.5) causes epithelium injury and endothelial dysfunction. Read More

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http://dx.doi.org/10.1038/s41598-019-40670-yDOI Listing
March 2019
1 Read

Effects of concentration of amyloid β (Aβ) on viability of cultured retinal pigment epithelial cells.

BMC Ophthalmol 2019 Mar 8;19(1):70. Epub 2019 Mar 8.

Department of Ophthalmology, Nara Medical University, 840 Shijo-cho, Kashihara, 634-8522, Japan.

Background: Amyloid beta (Aβ) is a constituent of drusen that is a common sign of age-related macular degeneration (AMD). The purpose of this study was to investigate the effect of Aβ on human retinal pigment epithelial (RPE) cells in culture.

Methods: Cells from a human RPE cell line (ARPE-19) were exposed to 0 to 25 μM of Aβ 1-40 for 48 h, and the number of living cells was determined by WST-8 cleavage. Read More

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http://dx.doi.org/10.1186/s12886-019-1076-3DOI Listing

Oxidative stress potential of the herbicides bifenox and metribuzin in the microalgae Chlamydomonas reinhardtii.

Aquat Toxicol 2019 Feb 27;210:117-128. Epub 2019 Feb 27.

Norwegian Institute for Water Research, Gaustadalléen 21, 0349 Oslo, Norway; Centre for Environmental Radioactivity, Norwegian University of Life Sciences (NMBU), Post box 5003, N-1432 Ås, Norway.

The widespread presence of herbicides in the aquatic environment has raised awareness about the need to develop further in depth ecotoxicological risk assessments, more specifically on potential effects on photosynthetic organisms as microalgae. The majority of the information available regarding the toxicity of herbicides towards microalgae is related to traditional toxicological and regulatory-relevant endpoints such as growth inhibition, leaving a significant gap on knowledge regarding underlying interactions and damage to biological targets. In this context, this study aimed to supplement the general toxicity information of bifenox and metribuzin in the microalgae Chlamydomonas reinhardtii using a battery of selected high-throughput methods. Read More

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http://dx.doi.org/10.1016/j.aquatox.2019.02.021DOI Listing
February 2019

The 308-nm excimer laser treatment does not increase the risk of skin cancer in patients with vitiligo: A population-based retrospective cohort study.

Pigment Cell Melanoma Res 2019 Mar 8. Epub 2019 Mar 8.

Department of Dermatology, Chungnam National University Hospital, Daejeon, Korea.

A 308-nm excimer laser (EL) has been widely used to treat patients with localized vitiligo. However, data are rare on the influence of EL treatment on the risks of skin cancer. To evaluate the skin cancer risks after long-term EL treatment, we performed a nationwide population-based retrospective cohort study using the Korean National Health Insurance Claims Database. Read More

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http://dx.doi.org/10.1111/pcmr.12781DOI Listing
March 2019
1 Read

Dyspigmented Hypertrophic Scars: beyond Skin Color.

Pigment Cell Melanoma Res 2019 Mar 8. Epub 2019 Mar 8.

Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, 110 Irving Street, NW, Washington, DC, 20010, USA.

Although pigment synthesis is well understood, relevant mechanisms of psychologically debilitating dyspigmentation in nascent tissue after cutaneous injuries are still unknown. Here, differences in genomic transcription of hyper- and hypopigmented tissue relative to uninjured skin were investigated using a red Duroc swine scar model. Transcription profiles differed based on pigmentation phenotypes with a trend of more upregulation or downregulation in hyper- or hypopigmented scars, respectively. Read More

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http://dx.doi.org/10.1111/pcmr.12780DOI Listing
March 2019
2 Reads

Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803.

Plant Cell Physiol 2019 Mar 7. Epub 2019 Mar 7.

Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku, FI-20014, Finland, Finland, phone: +358 50 430 4639, fax: +358 (0)29 450 (all authors: T.H., D.M.-P., E-M.A.).

NdbA, one of the three type 2 NAD(P)H dehydrogenases (NDH-2) in Synechocystis sp. PCC 6803 (hereafter Synechocystis) was here localized to the thylakoid membrane, unique for the three NDH-2s, and investigated with respect to photosynthetic and cellular redox metabolism. For this purpose, a deletion mutant (ΔndbA) and a complementation strain overexpressing NdbA (ΔndbA::ndbA) were constructed. Read More

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http://dx.doi.org/10.1093/pcp/pcz044DOI Listing

Evolution and Diversification of Genes in Solanaceae.

Front Plant Sci 2019 21;10:43. Epub 2019 Feb 21.

Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, United States.

Ecologically and economically important fleshy edible fruits have evolved from dry fruit numerous times during angiosperm diversification. However, the molecular mechanisms that underlie these shifts are unknown. In the Solanaceae there has been a major shift to fleshy fruits in the subfamily Solanoideae. Read More

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https://www.frontiersin.org/article/10.3389/fpls.2019.00043/
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http://dx.doi.org/10.3389/fpls.2019.00043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394111PMC
February 2019
4 Reads

The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.

Sci Rep 2019 Mar 7;9(1):3860. Epub 2019 Mar 7.

Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. Read More

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http://dx.doi.org/10.1038/s41598-019-40262-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405859PMC

Induced pluripotent stem cells-derived melanocyte precursor cells undergoing differentiation into melanocytes.

Pigment Cell Melanoma Res 2019 Mar 6. Epub 2019 Mar 6.

Division of Dermatology, Department of Internal Related, Graduate School of Medicine, Kobe University, Kobe, Japan.

Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in either lacking the melanosomal structure, or the melanocyte-specific marker genes MITF, TYR and SOX10. Read More

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http://dx.doi.org/10.1111/pcmr.12779DOI Listing

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis.

Sci Rep 2019 Mar 6;9(1):3752. Epub 2019 Mar 6.

Institute for Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Heraklion, Greece.

Development requires the implementation of a plethora of molecular mechanisms, involving a large set of genes to ensure proper cell differentiation, morphogenesis of tissues and organs as well as the growth of the organism. Genome duplication and resulting paralogs are considered to provide the raw genetic materials important for new adaptation opportunities and boosting evolutionary innovation. The present study investigated paralogous genes, involved in three-spined stickleback (Gasterosteus aculeatus) development. Read More

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http://dx.doi.org/10.1038/s41598-019-40127-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403355PMC

Characterization of CDNF-Secreting ARPE-19 Cell Clones for Encapsulated Cell Therapy.

Cell Transplant 2019 Mar 6:963689719827943. Epub 2019 Mar 6.

1 Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.

Cerebral Dopamine Neurotrophic Factor (CDNF) shows beneficial effects in rodent models of Parkinson's and Alzheimer's disease. The brain is a challenging target for protein therapy due to its exclusive blood-brain barrier. Hence, the therapeutic protein should be delivered directly to the brain parenchyma. Read More

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http://dx.doi.org/10.1177/0963689719827943DOI Listing

High-glucose induces retinal pigment epithelium mitochondrial pathways of apoptosis and inhibits mitophagy by regulating ROS/PINK1/Parkin signal pathway.

Biomed Pharmacother 2019 Mar 15;111:1315-1325. Epub 2019 Jan 15.

Department of Ophthalmology, The 2nd Affiliated Hospital of Kunming Medical University, Dianmian Road, Kunming Yunnan, China. Electronic address:

Diabetic retinopathy (DR) seriously endangers human beings' health, uncovering the underlying mechanism might help to cure DR. In this study, we found that the effects of glucose on retinal pigment epithelium (RPE) varies in a dose dependent manner, high-glucose (50mM) promotes reactive oxygen species (ROS) generation and cell apoptosis, inhibits cell mitophagy as well as proliferative abilities, while low-glucose (15mM) induces ROS production and cell mitophagy, but has little impacts on cell apoptosis and proliferation. Of note, the toxic effects of high-glucose (50mM) on RPE are alleviated by ROS scavengers and aggravated by autophagy inhibitor 3-methyladenine (3-MA) or mitophagy inhibitor cyclosporin A (CsA). Read More

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http://dx.doi.org/10.1016/j.biopha.2019.01.034DOI Listing

Drosophila CrebB is a Substrate of the Nonsense-Mediated mRNA Decay Pathway that Sustains Circadian Behaviors.

Mol Cells 2019 Mar 28. Epub 2019 Mar 28.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea.

Post-transcriptional regulation underlies the circadian control of gene expression and animal behaviors. However, the role of mRNA surveillance via the nonsense-mediated mRNA decay (NMD) pathway in circadian rhythms remains elusive. Here, we report that Drosophila NMD pathway acts in a subset of circadian pacemaker neurons to maintain robust 24h rhythms of free-running locomotor activity. Read More

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http://dx.doi.org/10.14348/molcells.2018.2451DOI Listing

Dicer1 is required for pigment cell and craniofacial development in zebrafish.

Biochim Biophys Acta Gene Regul Mech 2019 Mar 3;1862(4):472-485. Epub 2019 Mar 3.

Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, S2000EZP Rosario, Argentina. Electronic address:

The multidomain RNase III endoribonuclease DICER is required for the generation of most functional microRNAs (miRNAs). Loss of Dicer affects developmental processes at different levels. Here, we characterized the zebrafish Dicer1 mutant, dicer1, which has a single point mutation induced by N-ethyl-N-nitrosourea mutagenesis. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2019.02.005DOI Listing
March 2019
1 Read

Distribution and Genetic Diversity of Genes Involved in Quorum Sensing and Prodigiosin Biosynthesis in the Complete Genome Sequences of Serratia marcescens.

Genome Biol Evol 2019 Mar 6. Epub 2019 Mar 6.

Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University, Japan.

Quorum sensing is a cell density-dependent regulation of gene expression. N-Acyl-l-homoserine lactone (AHL) is a major quorum-sensing signaling molecule in gram-negative bacteria and synthesized by the LuxI family protein. The genus Serratia is known as a producer of the red pigment, prodigiosin, whose biosynthesis is dependent on the pig gene cluster. Read More

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http://dx.doi.org/10.1093/gbe/evz046DOI Listing

SOX10 regulates multiple genes to direct eumelanin vs. pheomelanin production in domestic rock pigeon.

Pigment Cell Melanoma Res 2019 Mar 6. Epub 2019 Mar 6.

Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, 84602, USA.

The domesticated rock pigeon (Columba livia) has been bred for hundreds of years to display an immense variety of ornamental attributes such as feather color and color patterns. Color is influenced by multiple loci that impact the type and amount of melanin deposited on the feathers. Pigeons homozygous for the "recessive red" mutation, which causes downregulation of Sox10, display brilliant red feathers instead of blue/black feathers. Read More

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http://dx.doi.org/10.1111/pcmr.12778DOI Listing

Countershading in zebrafish results from an Asip1 controlled dorsoventral gradient of pigment cell differentiation.

Sci Rep 2019 Mar 5;9(1):3449. Epub 2019 Mar 5.

Deparment of Biotechnology and Aquaculture. Instituto de Investigaciones Marinas, IIM-CSIC, Vigo, 36208, Spain.

Dorso-ventral (DV) countershading is a highly-conserved pigmentary adaptation in vertebrates. In mammals, spatially regulated expression of agouti-signaling protein (ASIP) generates the difference in shading by driving a switch between the production of chemically-distinct melanins in melanocytes in dorsal and ventral regions. In contrast, fish countershading seemed to result from a patterned DV distribution of differently-coloured cell-types (chromatophores). Read More

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http://www.nature.com/articles/s41598-019-40251-z
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http://dx.doi.org/10.1038/s41598-019-40251-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401153PMC
March 2019
3 Reads

Retinal dystrophy associated with Danon disease and pathogenic mechanism through LAMP2-mutated retinal pigment epithelium.

Eur J Ophthalmol 2019 Mar 5:1120672119832183. Epub 2019 Mar 5.

Department of Ophthalmology, The University of Tokyo Hospital, Tokyo, Japan.

Introduction:: Lysosome-associated membrane protein 2 plays an important role in autophagy and lysosomal function and its mutation is responsible for pathogenesis of Danon disease, which can cause retinopathy, though its pathophysiological contribution to retinal dysfunction remains unclear. The purpose of our research is to report the first case of Japanese Danon disease retinopathy and to understand how LAMP2 dysfunction contributes to pathogenesis of retinopathy.

Methods:: One case underwent ophthalmic examination including slit-lamp exam, fundus imaging, visual field testing, and electroretinogram. Read More

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http://dx.doi.org/10.1177/1120672119832183DOI Listing

Oxidative Stress and Dysfunctional Intracellular Traffic Linked to an Unhealthy Diet Results in Impaired Cargo Transport in the Retinal Pigment Epithelium (RPE).

Mol Nutr Food Res 2019 Mar 5:e1800951. Epub 2019 Mar 5.

E. Keeling, A. J. Lotery, J. A. Ratnayaka, Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, MP806, Tremona Road, SO16 6YD, UK.

Scope: Oxidative stress and dysregulated intracellular trafficking are associated with an unhealthy diet which underlies pathology. Here, we sought to study these effects on photoreceptor outer segment (POS) trafficking in the Retinal Pigment Epithelium (RPE), a major pathway of disease underlying irreversible sight-loss.

Methods And Results: POS trafficking was studied in ARPE-19 cells using an algorithm-based quantification of confocal-immunofluorescence data supported by ultrastructural studies. Read More

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http://dx.doi.org/10.1002/mnfr.201800951DOI Listing

Intravitreal Ets1 siRNA alleviates choroidal neovascularization in a mouse model of age-related macular degeneration.

Cell Tissue Res 2019 Mar 1. Epub 2019 Mar 1.

Department of Ophthalmology, Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu, China.

Choroidal neovascularization (CNV) is the basic feature of neovascular age-related macular degeneration (AMD), the leading cause of blindness in elders. Macrophages and microglia promote CNV via producing pro-angiogenic factors and inflammatory cytokines. Transcription factor E26 transformation specific-1 (Ets1) plays a pro-angiogenic role via its pro-inflammatory function. Read More

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http://dx.doi.org/10.1007/s00441-019-03001-1DOI Listing

Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells.

Iran J Basic Med Sci 2019 Feb;22(2):128-133

Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Objectives: It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Read More

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http://ijbms.mums.ac.ir/article_12011.html
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http://dx.doi.org/10.22038/ijbms.2018.25023.6214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396987PMC
February 2019
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AAV -regulatory sequences are correlated with ocular toxicity.

Proc Natl Acad Sci U S A 2019 Mar 4. Epub 2019 Mar 4.

Department of Genetics, Harvard Medical School, Boston, MA 02115;

Adeno-associated viral vectors (AAVs) have become popular for gene therapy, given their many advantages, including their reduced inflammatory profile compared with that of other viruses. However, even in areas of immune privilege such as the eye, AAV vectors are capable of eliciting host-cell responses. To investigate the effects of such responses on several ocular cell types, we tested multiple AAV genome structures and capsid types using subretinal injections in mice. Read More

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http://dx.doi.org/10.1073/pnas.1821000116DOI Listing

MroQ is a Novel Abi-domain Protein That Influences Virulence Gene Expression in via Modulation of Agr Activity.

Infect Immun 2019 Mar 4. Epub 2019 Mar 4.

Department of Cell Biology, Microbiology & Molecular Biology, University of South Florida, Tampa, Florida, USA

Numerous factors have to date been identified as playing a role in the regulation of Agr activity in , including transcription factors, antisense RNAs, and host elements. Herein we investigate the product of SAUSA300_1984 (termed MroQ), a transmembrane Abi-domain/M79 protease-family protein, as a novel effector of this system. Using a USA300 mutant we observed a drastic reduction in proteolysis, hemolysis and pigmentation that was fully complementable. Read More

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http://dx.doi.org/10.1128/IAI.00002-19DOI Listing

Early warning method for cyanobacteria toxin, taste and odor problems by the evaluation of fluorescence signals.

Sci Total Environ 2019 Feb 20;667:681-690. Epub 2019 Feb 20.

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany.

Permanganate and ozone are often used in drinking water treatment plants for the oxidation of taste and odor compounds, toxins, and algae as well as the reduction of mussel activity. The disadvantage of an overuse of such oxidants is the potential lysis of cyanobacterial cells. Cell lysis causes taste and odor components as well as toxins to be released into the water, which results in the need for even more treatment to remove these compounds completely. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.02.271DOI Listing
February 2019

Asymptomatic malignant melanoma of the gallbladder with multiple brain metastases diagnosed with endoscopic ultrasound-guided fine-needle aspiration cytology.

Clin J Gastroenterol 2019 Mar 4. Epub 2019 Mar 4.

Department of Surgery, Fujimoto General Hospital, 17-1, Hayasuzu, Miyakonojo, Miyazaki, 885-0055, Japan.

Malignant melanoma of the gallbladder (MMG) is extremely rare and its early stage diagnosis is difficult. Most reports of MMG describe metastatic tumors. We herein report a rare case of presumed primary MMG diagnosed by endoscopic ultrasonography-guided fine-needle aspiration (EUS-FNA) cytology without surgical resection. Read More

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http://dx.doi.org/10.1007/s12328-019-00957-zDOI Listing
March 2019
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From neural crest cells to melanocytes: cellular plasticity during development and beyond.

Cell Mol Life Sci 2019 Mar 4. Epub 2019 Mar 4.

Molecular and Cellular Oncology Laboratory, Department of Biomedical Molecular Biology, Ghent University, Technologiepark-Zwijnaarde 71, 9052, Ghent, Belgium.

Here, we review melanocyte development and how the embryonic melanoblast, although specified to become a melanocyte, is prone to cellular plasticity and is not fully committed to the melanocyte lineage. Even fully differentiated and pigment-producing melanocytes do not always have a stable phenotype. The gradual lineage restriction of neural crest cells toward the melanocyte lineage is determined by both cell-intrinsic and extracellular signals in which differentiation and pathfinding ability reciprocally influence each other. Read More

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http://dx.doi.org/10.1007/s00018-019-03049-wDOI Listing

Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy.

Mol Ther Nucleic Acids 2019 Feb 2;16:38-50. Epub 2019 Feb 2.

Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark; Department of Ophthalmology, Aarhus University Hospital, 8000 Aarhus C, Denmark. Electronic address:

Vascular endothelial growth factor A (VEGFA) is involved in the pathogenesis of vasoproliferative retinal diseases, such as exudative age-related macular degeneration (AMD). The objective of this study was to investigate whether dual-acting therapy based on the simultaneous expression of anti-VEGFA microRNAs (miRNAs) and the secreted, antiangiogenic protein pigment endothelial-derived factor (PEDF) delivered by adeno-associated virus (AAV) vectors provides improved protection against choroidal neovascularization (CNV). To investigate this, a multigenic AAV vector allowing retina pigment epithelium (RPE)-specific expression of anti-VEGFA miRNAs and PEDF was engineered. Read More

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http://dx.doi.org/10.1016/j.omtn.2019.01.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393707PMC
February 2019

Acute (gangrenous) esophageal necrosis (black esophagus).: A rare form of injury with specific histologic features and diverse clinical associations with a common pathogenesis.

Hum Pathol 2019 Feb 27. Epub 2019 Feb 27.

Unidad de Patología, Hospital General de Mexico, Mexico City, Mexico.

Acute esophageal necrosis (AEN) also known as black esophagus is a rare form of injury to the esophageal mucosa that complicates a variety of clinical conditions. It is characterized by circumpherential black discoloration of the mucosa. There is little information relating to the histopathologic features and pathogenesis of this condition. Read More

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http://dx.doi.org/10.1016/j.humpath.2019.02.003DOI Listing
February 2019

Regulation of conidiation, dimorphic transition, and microsclerotia formation by MrSwi6 transcription factor in dimorphic fungus Metarhizium rileyi.

World J Microbiol Biotechnol 2019 Mar 1;35(3):46. Epub 2019 Mar 1.

School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, People's Republic of China.

Microsclerotia (MS) produced in the liquid culture of the dimorphic insect pathogen Metarhizium rileyi can be used as a mycoinsecticide. Bioinformatics analysis demonstrated that the cell cycle signaling pathway was involved in regulating MS formation. To investigate the mechanisms by which the signaling pathway is regulated, a cell cycle box binding transcription factor MrSwi6 of M. Read More

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http://dx.doi.org/10.1007/s11274-019-2619-8DOI Listing

Dynamic pigmentary and structural coloration within cephalopod chromatophore organs.

Nat Commun 2019 Mar 1;10(1):1004. Epub 2019 Mar 1.

Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, 02115, USA.

Chromatophore organs in cephalopod skin are known to produce ultra-fast changes in appearance for camouflage and communication. Light-scattering pigment granules within chromatocytes have been presumed to be the sole source of coloration in these complex organs. We report the discovery of structural coloration emanating in precise register with expanded pigmented chromatocytes. Read More

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http://dx.doi.org/10.1038/s41467-019-08891-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397165PMC
March 2019
1 Read